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SRX26871407: Genome sequences for Pseudomonas syringae strains with characterized LPS
1 ILLUMINA (NextSeq 2000) run: 847,614 spots, 251.5M bases, 87.6Mb downloads

Design: A single colony of the strain was picked to Kb media and grown overnight at 27 degrees C without antibiotics. Genomic DNA was extracted from this culture using a Promega Wizard Kit (Madison, WI), without RNAse addition. Genomic DNA was sent to SeqCoast for standard Illumina sequencing.Samples were prepared for whole genome sequencing using an Illumina DNA Prep tagmentation kit and unique dual indexes.Sequencing was performed on the Illumina NextSeq2000 platform using a 300 cycle flow cell kit to produce 2x150bp paired reads. 1-2% PhiX control was spiked into the run to support optimal base calling.Read demultiplexing, read trimming, and run analytics were performed using DRAGEN v3.10.12, an on-board analysis software on the NextSeq2000. We include fastqc metrics as a best practice and for examination in the case of unexpected outputs.
Submitted by: University of Arizona
Study: Genome sequences for Pseudomonas syringae strains with characterized LPS
PRJNA1128096 • SRP547493 • All experiments • All runs
show Abstracthide Abstract
A variety of strains of Pseudomonas syringae, each with previously characterized lipopolysaccharide chains, were obtained from multiple stock centers for genome sequencing. This study will help to link LPS diversity with underlying genetic pathways.
Sample: NCPPB79-draft
SAMN42036865 • SRS23353046 • All experiments • All runs
Organism: Pseudomonas ficuserectae
Library:
Name: NCPPB79
Instrument: NextSeq 2000
Strategy: WGS
Source: GENOMIC
Selection: RANDOM
Layout: PAIRED
Runs: 1 run, 847,614 spots, 251.5M bases, 87.6Mb
Run# of Spots# of BasesSizePublished
SRR31503468847,614251.5M87.6Mb2024-11-25

ID:
36296917

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