SALKO - SRA

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SRX25927579: SALKO
1 ILLUMINA (Illumina NovaSeq 6000) run: 7.7M spots, 2.3G bases, 706Mb downloads

Design: Total RNA was extracted from Streptomyces strains SALKO using the TRIzol reagent (Invitrogen) according to the manufacturer's protocol. The quality and integrity of the RNA samples were evaluated using a NanoDrop spectrophotometer and agarose gel electrophoresis. Ribosomal RNA was removed using the Ribo-Zero rRNA Removal Kit (Illumina) to enrich for mRNA. Sequencing libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina (New England Biolabs), following the recommended protocol. In brief, the enriched mRNA was fragmented and reverse transcribed into cDNA. The cDNA was then end-repaired, A-tailed, and ligated to Illumina sequencing adapters. The libraries were amplified by PCR and purified with AMPure XP beads (Beckman Coulter). Library quality was assessed using an Agilent 2100 Bioanalyzer, and the final libraries were quantified using a Qubit Fluorometer (Thermo Fisher Scientific). Paired-end sequencing (150 bp) was performed on an Illumina NovaSeq 6000 platform.

Submitted by: zhejiang univercity

Study: Daunorubicin heterologous host comparison

PRJNA1155031 • SRP529889 • All experiments • All runs

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This study investigates the transcriptomic profiles of two Streptomyces strains, ZD11-M4 and ZD11-SALKO, to understand the molecular mechanisms underlying daunorubicin production and the associated stress responses. Strain M4 is a high-yield daunorubicin-producing mutant derived from the parental strain ZD11-SALKO, while SALKO is a non-producing control.

Sample:

SAMN43440503 • SRS22514991 • All experiments • All runs

Organism: Streptomyces albus

Library:

Name: SALKO

Instrument: Illumina NovaSeq 6000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Runs: 1 run, 7.7M spots, 2.3G bases, 706Mb

Run	# of Spots	# of Bases	Size	Published
SRR30503778	7,740,373	2.3G	706Mb	2024-09-01

ID:: 35001233

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