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SRX26801417: Full-length 16S rRNA Seq
1 OXFORD_NANOPORE (MinION) run: 33,657 spots, 44.6M bases, 38.7Mb downloads

Design: null specific
Submitted by: KU Leuven
Study: Ambr 8 - Exploration of perturbations on 3-species synthetic human gut community
show Abstracthide Abstract
Here we explored the effect of two perturbations on the stability of a synthetic human gut bacterial community grown in microbioreactors in emulated chemostat mode. The (type)strains used were: Roseburia intestinalis (DSM 14610), Blautia hydrogenotrophica (DSM 10507), Bacteroides thetaiotaomicron (DSM 2079). We first applied a pH perturbation by decreasing the pH of the feed (WC with pH 6.4 to pH 3.38) between t97 and t109 (24 hours total). Subsequently, we applied a perturbation in the dilution rate by stopping the feed for 12 hours between t251 and t263 (periodic removal of liquid continued), after which an additional 5 mL fresh medium (50% of total volume) was added to the vessels.
Sample:
SAMN44855960 • SRS23287142 • All experiments • All runs
Library:
Name: Ambr8_P3_92
Instrument: MinION
Strategy: AMPLICON
Source: SYNTHETIC
Selection: PCR
Layout: SINGLE
Runs: 1 run, 33,657 spots, 44.6M bases, 38.7Mb
Run# of Spots# of BasesSizePublished
SRR3143258433,65744.6M38.7Mb2024-12-20

ID:
36213120

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