Isolate from Bifidobacterium breve DSM 20213 = JCM 1193 - SRA

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SRX2033245: Isolate from Bifidobacterium breve DSM 20213 = JCM 1193
1 ION_TORRENT (Ion Torrent PGM) run: 123,729 spots, 31.1M bases, 18.7Mb downloads

Design: Genomic library preparation was done using the Ion PGM Sequencing 400 kit (Life Technologies). Genomic DNA of the strains were fragmented by sonication. The overall fragment length was checked by an Agilent 2100 Bioanalyzer with the High Sensitivity DNA Kit (Agilent Technologies, CA, USA). Fragments of ~400 bp were selected with E-GelŽ SizeSelect 2% agarose gels (Thermo Fisher). The selected libraries were amplified for eight cycles with the reagents supplied in Ion Plus Fragment Kit (Thermo Fisher) and were then purified by using the Agencourt AMPure XP DNA purification beads (Beckman Coulter Genomics, Germany) in order to remove primer dimers. From the concentration and the average size of each amplicon, the amount of DNA fragments per microliter was calculated and libraries created by using the Ion Plus Fragment Library kit (Life Technologies, Italy).

Submitted by: The Chinese University of Hong Kong

Study: Bifidobacterium breve strain:JCM1192 Genome sequencing

PRJNA339211 • SRP082348 • All experiments • All runs

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To elucidate the utilization mechanism of insoluble yeast glucan by B. breve mutant studies

Sample: Isolate from Bifidobacterium breve DSM 20213 = JCM 1192

SAMN05590114 • SRS1627231 • All experiments • All runs

Organism: Bifidobacterium breve DSM 20213 = JCM 1192

Library:

Name: BBM2

Instrument: Ion Torrent PGM

Strategy: WGS

Source: GENOMIC

Selection: RANDOM

Layout: SINGLE

Runs: 1 run, 123,729 spots, 31.1M bases, 18.7Mb

Run	# of Spots	# of Bases	Size	Published
SRR4042123	123,729	31.1M	18.7Mb	2017-02-04

ID:: 2937183

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