Isolate from Bifidobacterium breve DSM 20213 = JCM 1195 - SRA

Links from BioSample

SRX2033247: Isolate from Bifidobacterium breve DSM 20213 = JCM 1195
1 ION_TORRENT (Ion Torrent PGM) run: 302,134 spots, 72.6M bases, 38.9Mb downloads

Design: Genomic library preparation was done using the Ion PGM Sequencing 400 kit (Life Technologies). Genomic DNA of the strains were fragmented by sonication. The overall fragment length was checked by an Agilent 2100 Bioanalyzer with the High Sensitivity DNA Kit (Agilent Technologies, CA, USA). Fragments of ~400 bp were selected with E-GelŽ SizeSelect 2% agarose gels (Thermo Fisher). The selected libraries were amplified for eight cycles with the reagents supplied in Ion Plus Fragment Kit (Thermo Fisher) and were then purified by using the Agencourt AMPure XP DNA purification beads (Beckman Coulter Genomics, Germany) in order to remove primer dimers. From the concentration and the average size of each amplicon, the amount of DNA fragments per microliter was calculated and libraries created by using the Ion Plus Fragment Library kit (Life Technologies, Italy).

Submitted by: The Chinese University of Hong Kong

Study: Bifidobacterium breve strain:JCM1192 Genome sequencing

PRJNA339211 • SRP082348 • All experiments • All runs

show Abstracthide Abstract

To elucidate the utilization mechanism of insoluble yeast glucan by B. breve mutant studies

Sample: Isolate from Bifidobacterium breve DSM 20213 = JCM 1192

SAMN05590116 • SRS1627233 • All experiments • All runs

Organism: Bifidobacterium breve DSM 20213 = JCM 1192

Library:

Name: BBR7

Instrument: Ion Torrent PGM

Strategy: WGS

Source: GENOMIC

Selection: RANDOM

Layout: SINGLE

Runs: 1 run, 302,134 spots, 72.6M bases, 38.9Mb

Run	# of Spots	# of Bases	Size	Published
SRR4042125	302,134	72.6M	38.9Mb	2017-02-04

ID:: 2937185

SRA

Sequence Read Archive

Result Filters

Send to:

Links from BioSample

Supplemental Content

Related information

Recent activity