Instrument: Illumina HiSeq 2000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: SINGLE
Construction protocol: Lyophilized mycelia were ground in liquid nitrogen and total RNA was extracted using the "RNeasy Plant Mini Kit" (Qiagen, Courtaboeuf, France). PEG 8000 (20 mg.ml-1) and β-mercaptoethanol (10 µl.ml-1) were added in the RLC buffer (Qiagen). Samples were subsequently purified using the "Total RNA Purification, NucleoBond® RNA/DNA 80" kit (Macherey-Nagel, Hoerdt, France) by following the manufacturer’s recommendations. cDNA libraries were prepared for sequencing using standard Illumina protocols by the Joint Genome Institue (JGI)