Miseq of Teleogryllus oceanicus gut bacteria: lab male fed artificial... - SRA

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SRX2786048: Miseq of Teleogryllus oceanicus gut bacteria: lab male fed artificial diet sample 2
1 ILLUMINA (Illumina MiSeq) run: 15,932 spots, 4M bases, 1.7Mb downloads

Design: 16S rRNA amplicons were generated in a single amplification step using primers 515F (5- GTGCCAGCMGCCGCGGTAA -3) and R806 (5- GGACTACHVGGGTWTCTAAT -3). The amplicon library was prepared by pooling PCR products into equimolar ratios based on qPCR and quantification using a Labchip GX Touch HT (Perkin Elmer). Amplicons in the library were size-selected using a Pippin Prep (Sage Science) and purified using the Qiaquick PCR Purification Kit (Qiagen). The volume of purified library added for sequencing was determined using a Labchip GX Touch HT (Perkin Elmer), and sequenced (uni-directionally) using a 300 cycle MiSeq v2 Reagent Kit and standard flow cell on an Illumina MiSeq platform.

Submitted by: University of Western Australia

Study: Teleogryllus oceanicus Raw sequence reads

PRJNA384097 • SRP106500 • All experiments • All runs

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Examination of gut bacterial communities of wild caught field crickets and lab-reared crickets fed either cat chow or chemically defined artificial diets

Sample: Artificial_diet_male_sample_2

SAMN06887807 • SRS2168077 • All experiments • All runs

Organism: Teleogryllus oceanicus

Library:

Name: ADM13

Instrument: Illumina MiSeq

Strategy: AMPLICON

Source: GENOMIC

Selection: PCR

Layout: SINGLE

Runs: 1 run, 15,932 spots, 4M bases, 1.7Mb

Run	# of Spots	# of Bases	Size	Published
SRR5512075	15,932	4M	1.7Mb	2017-10-18

ID:: 4016385

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