WGS of Pseudomonas savastanoi pv. fraxini NCPPB1006 - SRA

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SRX2876149: WGS of Pseudomonas savastanoi pv. fraxini NCPPB1006
1 ILLUMINA (Illumina Genome Analyzer IIx) run: 13.8M spots, 1.4G bases, 1Gb downloads

Design: Genomic DNA from Pseudomonas savastanoi pv. fraxini NCPPB1006 was extracted from bacterial cells grown overnight in LB medium at 28ĄC. DNA was extracted using Genomic DNA Purification JETFLEX kit (Genomed GmbH, Lhne, Germany) following the manufacturerŐs instructions. The DNA sample was further purified by a first extraction with phenol:chloroform:isoamyl alcohol (25:24:1), followed by a second extraction with chloroform:isoamyl alcohol (24:1). DNA was precipitated with 1/10 volume of 3M sodium acetate and two volumes of 100% ethanol and resuspended in MilliQ water. NanoDrop measurements gave a concentration of 375,5 ng/ľL (in total 9,5 ľg of DNA) with A260/A280 of 1.96 and A260/230 of 2.46. The draft genome of this strain was sequenced using Illumina Genome Analyzer IIx at "Centro de Investigacin Biomdica de La RiojaÓ (CIBIR), Logroo, Spain.

Submitted by: Universidad de Malaga

Study: Pseudomonas savastanoi pv. fraxini strain:NCPPB 1006 Genome sequencing and assembly

PRJNA388451 • SRP108400 • All experiments • All runs

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The general purpose of this sequencing project is to perform a comparative analysis of the diverse Pseudomonas savastanoi strains belonging to different pathovars (different host range)

Sample:

SAMN07177411 • SRS2245985 • All experiments • All runs

Organism: Pseudomonas savastanoi pv. fraxini

Library:

Name: Psf_NCPPB1006

Instrument: Illumina Genome Analyzer IIx

Strategy: WGS

Source: GENOMIC

Selection: unspecified

Layout: PAIRED

Runs: 1 run, 13.8M spots, 1.4G bases, 1Gb

Run	# of Spots	# of Bases	Size	Published
SRR5637676	13,780,404	1.4G	1Gb	2017-09-18

ID:: 4117940

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