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SRX3424227: GSM2865707: STM815 wt nitrogen replete_1; Paraburkholderia phymatum STM815; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2500) run: 47M spots, 4.8G bases, 2.4Gb downloads

Submitted by: NCBI (GEO)
Study: In-nodule transcriptome analysis of Paraburkholderia phymatum during symbiosis with Phaseolus vulgaris
show Abstracthide Abstract
Paraburkholderia phymatum belongs to the ß-subclass of proteobacteria. It has recently been shown to be able to nodulate and fix nitrogen in symbiosis with several mimosoid and papillionoid legumes. In contrast to symbiosis of legumes with a-proteobacteria, very little is known about the molecular determinants underlying the successful establishment of this mutualistic relationship with ß-proteobacteria. In this study, we analyzed RNA-seq data of free-living P. phymatum growing under nitrogen replete and limited conditions, the latter partially mimicking the situation in nitrogen deprived soils. Among the genes up-regulated under nitrogen limitation, we found genes involved in exopolysaccharide production and motility, two traits relevant for plant root infection. Next, RNA-seq data of P. phymatum grown under free-living conditions and from symbiotic root nodules of Phaseolus vulgaris (common bean) were generated and compared. Among the genes highly up-regulated during symbiosis, we identified an operon encoding a potential cytochrome o ubiquinol oxidase (Bphy_3646-49). Bean root nodules induced by a cyoB mutant strain showed reduced nitrogenase and nitrogen fixation abilities suggesting an important role of the cytochrome for respiration inside the nodule. Analysis of mutant strains for RNA polymerase transcription factor rpoN (s54) and its activator NifA indicated that – similar to the situation in a-rhizobia – P. phymatum RpoN and NifA are key regulators during symbiosis with P. vulgaris. Overall design: Unraveling the molecular basis of the nitrogen-fixing symbiosis between P. vulgaris and P. phymatum.
Sample: STM815 wt nitrogen replete_1
SAMN08102244 • SRS2715858 • All experiments • All runs
Library:
Instrument: Illumina HiSeq 2500
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: SINGLE
Construction protocol: Cells were flash frozen on dry ice, and RNA was harvested using hot phenol protocol (Pessi et al 2007) . For RNA extracted from nodules, first nodules were disrupted with TissueLyzer and then rRNA of plants was removed using Ribo-zero kit (Epicentre). The cDNA was produced and nugen libraries obtained. RNA libraries were prepared using Encore Complete Prokaryotic RNA-Seq DR Muliplex System
Experiment attributes:
GEO Accession: GSM2865707
Links:
Runs: 1 run, 47M spots, 4.8G bases, 2.4Gb
Run# of Spots# of BasesSizePublished
SRR632447747,048,3624.8G2.4Gb2018-02-25

ID:
4768310

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