SRX3686944: RNA-seq of Nothoprocta perdicaria: adult
1 ILLUMINA (NextSeq 500) run: 41.9M spots, 6.3G bases, 2.7Gb downloads
Design: Flash frozen tissues stored in RNAlater at -80 C were homogenized with a Tissumizer (Tekmar). Total RNA was extracted from each specimen using the RNeasy mini kit (Qiagen). RNA samples were processed with a PrepX PolyA kit (Wafergen Biosystems) on the Apollo 324 (IntegenX) to enrich for polyadenylated transcripts. Stranded RNA-seq libraries were generated from these samples using the PrepX mRNA 48 kit (Wafergen Biosystems) on the Apollo 324.
Submitted by: Harvard University
Study:
Transcriptome sequencing for genome annotation in palaeognathsshow Abstracthide AbstractTranscriptome sequencing from multiple tissues from emu (Dromaius novaehollandiae) and Chilean tinamou (Nothoprocta perdicaria) to aid in genome annotation.
Sample:
NOTE: sample mislabeled as emu, determined to be Nothoprocta perdicaria by sequence identity, but unknown sex and tissueLibrary:
Name: emu(ctin)_UNK
Instrument: NextSeq 500
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: PolyA
Layout: PAIRED
Runs:
1 run, 41.9M spots, 6.3G bases, 2.7Gb