Whole genome sequencing of Shiga toxin-producing Escherichia coli str... - SRA

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SRX3895580: Whole genome sequencing of Shiga toxin-producing Escherichia coli strains isolated from different sources
1 ILLUMINA (Illumina MiSeq) run: 2.3M spots, 1.1G bases, 601.6Mb downloads

Design: DNA was extracted from a single-separated colony using the DNeasy Blood & Tissue Kit (QIAgen) in a QIAcube instrument (QIAgen). Fragmentation was performed using microTUBE-15 AFA Beads Screw-Cap (Covaris) and a M220 Focused-ultrasonicator (Covaris) with default settings to achieve a 350 bp peak. The sheared DNA was used for library construction using the Accel-NGS 2S Plus DNA Library Kit (Swift Bioscience) and paired-end sequenced using an Illumina MiSeq platform with MiSeq Reagent Kit v2 (2_250).

Submitted by: USDA

Study: Shiga toxin-producing Escherichia coli genome sequencing

PRJNA383591 • SRP115261 • All experiments • All runs

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Whole genome sequencing of Shiga toxin-producing Escherichia coli strains isolated from different sources to understand how pathogens respond to food-related stresses and cause disease, and to identify biomarkers.

Sample:

SAMN08810301 • SRS3133030 • All experiments • All runs

Organism: Escherichia coli

Library:

Name: MFS_26

Instrument: Illumina MiSeq

Strategy: WGS

Source: GENOMIC

Selection: RANDOM

Layout: PAIRED

Runs: 1 run, 2.3M spots, 1.1G bases, 601.6Mb

Run	# of Spots	# of Bases	Size	Published
SRR6952386	2,260,490	1.1G	601.6Mb	2019-04-04

ID:: 5349308

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