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ERX2579723: Illumina MiSeq paired end sequencing; Whole genome sequencing of isolate R0006_KLMC4
1 ILLUMINA (Illumina MiSeq) run: 257,544 spots, 91M bases, 55.6Mb downloads

Design: Isolate subcultured onto TCBS agar and incubated at 37°C for 24 hours. Colonies from TCBS were used for Genomic DNA extraction. Genomic DNA was extracted using MasterPureTM Complete DNA and RNA Purification Kit (Epicentre, Illumina Inc.). The quality and quantity of genomic DNA were accessed using Qubit 2.0 fluorometer (Invitrogen, Life Technologies). DNA library preparation was done using Nextera XT DNA preparation kit (Illumina Inc.).
Submitted by: Kilimanjaro Clinical Research Institute
Study: Aeromonas caviae mimicking Vibrio cholerae infectious enteropathy in a cholera-endemic region
show Abstracthide Abstract
We report two cases with clinically mild cholera-like symptoms, at a time when a cholera outbreak was unfolding in other regions of the country (Tanzania). Initial microbiological testing determined colonies isolated from stool to be Vibrio cholerae, and warranted the conclusion "presumptive cholera". Whole genome sequencing established the presence of Aeromonas caviae rather than V. cholerae. The co-existence of Aeromonas species with V. cholerae in cholera-endemic regions suggests the possibility that a proportion of suspected cholera cases may be Aeromonas infections.
Sample: Aeromonas caviae strain KLMC4
SAMEA4644084 • ERS2464732 • All experiments • All runs
Library:
Instrument: Illumina MiSeq
Strategy: WGS
Source: GENOMIC
Selection: RANDOM
Layout: PAIRED
Construction protocol: Nextera XT
Experiment attributes:
library preparation date: 2015-09-11
Runs: 1 run, 257,544 spots, 91M bases, 55.6Mb
Run# of Spots# of BasesSizePublished
ERR2561379257,54491M55.6Mb2018-05-06

ID:
5522614

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