SRX6480123: WGS of Staphylococcus aureus
1 PACBIO_SMRT (PacBio RS II) run: 157,985 spots, 1.2G bases, 280.1Mb downloads
Design: Isolate grown overnight under nonselective conditions. Cells underwent high molecular weight DNA extraction using the Qiagen DNeasy Blood & Tissue Kit (Qiagen, 69504) according to the manufacturer's instructions, with modified lysis conditions. Bacterial cells were lysed by suspending cells in 3 L of 100 mg/ml RNase A (Ambion, AM2286) and ten L of 100 mg/ml lysozyme (Sigma, L1667-1G) for 30 minutes at 37C, followed by incubation with Proteinase K for one hour at 56C and two rounds of bead beating of one min each using 01mm silica beads (MP Bio).
Submitted by: Icahn School of Medicine
Study:
The Mount Sinai Pathogen Surveillance Programshow Abstracthide AbstractPrecision Pathogen Surveillance by Whole-Genome Sequencing.
Library:
Name: ER01518
Instrument: PacBio RS II
Strategy: WGS
Source: GENOMIC
Selection: RANDOM
Layout: SINGLE
Runs:
1 run, 157,985 spots, 1.2G bases, 280.1Mb