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SRX6867974: DNA-Seq of S. warneri: Buffalo milk
1 PACBIO_SMRT (PacBio RS II) run: 142,571 spots, 1.5G bases, 982.4Mb downloads

Design: The construction of SMRTbell libraries suitable for generating high accuracy long reads on the Sequel Systems requires 15 g of high molecular weight genomic DNA (gDNA). The DNA is sheared to a mode of 15 kb using Diagenodes Megaruptor. SMRTbell libraries are prepared from sheared gDNA using PacBios SMRTbell Template Prep Kit 1.0. SMRTbell libraries are then size-fractionated using Sage Sciences SageELF. The fractions suitable for sequencing have insert sizesfrom 10 kb to 15 kb.
Submitted by: Sao Paulo State University (Unesp), School of Agricultural and Veterinarian Sciences, Jaboticabal
Study: Staphylococcus saprophyticus strain:1A Genome sequencing
PRJNA482667 • SRP222367 • All experiments • All runs
show Abstracthide Abstract
Elucidating the virulence and antimicrobial resistance mechanisms of Staphylococcus spp. associated with bubaline mastitis, through "single-molecule, real-time sequencing" and comparative genomics
Sample:
SAMN09714427 • SRS5405407 • All experiments • All runs
Organism: Staphylococcus warneri
Library:
Name: SW16A
Instrument: PacBio RS II
Strategy: WGS
Source: GENOMIC
Selection: RT-PCR
Layout: SINGLE
Runs: 1 run, 142,571 spots, 1.5G bases, 982.4Mb
Run# of Spots# of BasesSizePublished
SRR10140037142,5711.5G982.4Mb2019-09-19

ID:
9038710

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