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SRX1984829: GSM2254467: mESC_stranded_RNASeq; Mus musculus; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2000) run: 42M spots, 8.5G bases, 5.1Gb downloads

Submitted by: NCBI (GEO)
Study: Co-assembly of stranded and unstranded RNA-seq data improves coding and noncoding transcriptome maps
show Abstracthide Abstract
We want to develop transcriptome assembly pipeline that significantly improves the quality of the assemblies constructed using stranded and/or unstranded RNA-seq data. Overall design: Transcriptome of mouse embryonic stem cells (mESC) were assembled using stranded and unstranded library generated by Illumina HiSeq 2000
Sample: mESC_stranded_RNASeq
SAMN05452818 • SRS1590090 • All experiments • All runs
Organism: Mus musculus
Library:
Instrument: Illumina HiSeq 2000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: RNA from mES cells was extracted using an RNeasy kit (Qiagen, USA). Polyadenylated RNA was isolated using Oligo dT beads (Invitrogen, USA). Illumina Truseq stranded and unstranded mRNA library prep kits (Illuminca, USA) were used for deep sequencing library preparation from 6ug of total RNA according to the manufacturer's protocol.
Experiment attributes:
GEO Accession: GSM2254467
Links:
Runs: 1 run, 42M spots, 8.5G bases, 5.1Gb
Run# of Spots# of BasesSizePublished
SRR397437842,008,6138.5G5.1Gb2016-08-01

ID:
2856623

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