show Abstracthide AbstractBlastomycosis has been reported from countries in Africa and the Middle East, but a decades-long debate has persisted regarding whether this is the same disease known in North America and caused by Blastomyces dermatitidis and B. gilchristii and the difference with the disease caused by recently described Blastomyces species, B. percursus and B. emzantsi. We sequenced and characterized isolates causing blastomycosis in Africa and the Middle East.