Shotgun genome sequencing of Togninia minima (Phaeoacremonium aleophi... - SRA

SRX220948: Shotgun genome sequencing of Togninia minima (Phaeoacremonium aleophilum)
1 ILLUMINA (Illumina HiSeq 2000) run: 27.9M spots, 5.6G bases, 3.7Gb downloads

Design: Genomic DNA was extracted mycelia growing on PDA plate. A single DNA library was prepared from 5 ug of total genomic DNA that was randomly fragmented using the Bioruptor sonicator (Diagenode). The library was prepared with the NEB Next DNA Sample Prep Master Mix according to manufacturer's instructions (New England Biolabs, MA USA). Fragments were purified using the Nucleotrap kit (Clontech) and eluted in 30 ul elution buffer and PCR-enriched using 14 PCR cycles. Paired end adapters and PCR primers were obtained from Illumina. Library quality and quantity were confirmed before sequencing using a Bioanalyzer (Agilent).

Submitted by: UC Davis

Study: Phaeoacremonium minimum UCRPA7 Genome sequencing and assembly

PRJNA188116 • SRP018340 • All experiments • All runs

show Abstracthide Abstract

Togninia minima (Phaeoacremonium aleophilum) is a fungal pathogen that causes esca and grapevine decline in California.

Sample: Togninia minima (Phaeoacremonium aleophilum) - isolate PA7

SAMN01908518 • SRS388652 • All experiments • All runs

Organism: Togninia minima UCRPA7

Library:

Instrument: Illumina HiSeq 2000

Strategy: WGS

Source: GENOMIC

Selection: unspecified

Layout: PAIRED

Spot descriptor:

1 forward101 reverse

Runs: 1 run, 27.9M spots, 5.6G bases, 3.7Gb

Run	# of Spots	# of Bases	Size	Published
SRR654175	27,947,048	5.6G	3.7Gb	2013-05-16

ID:: 308694

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