Genome sequencing of Streptomyces kebangsaanensis: an endophyte isola... - SRA

SRX2771637: Genome sequencing of Streptomyces kebangsaanensis: an endophyte isolated from Portulaca oleracea
1 ILLUMINA (Illumina HiSeq 2000) run: 13.1M spots, 2.6G bases, 1.8Gb downloads

Design: Genomic DNA was fragmented using Covaris S220 (Covaris Inc, USA) to a targeted size of 400 600 bp. The fragmented DNA was then end-repaired, ligated to Illumina TruSeq adapters, and PCR-enriched using TruSeq DNA Sample Preparation Kit (Illumina, USA) according to manufacturers protocol. The final sequencing library was quantified using KAPA kit (KAPA Biosystem, USA) on Agilent Stratagene Mx-3005p quantitative PCR machine (Agilent, USA) and library size was confirmed using Agilent Bioanalyzer High Sensitivity DNA Chip (Agilent, USA). The resulting library was sequenced using an Illumina flow cell, and 202 cycles on the Illumina HiSeq 2000 platform (Illumina, USA). The sequencing run generated more than a total of 2.6 GB of raw data.

Submitted by: The National University of Malaysia

Study: Streptomyces kebangsaanensis strain:SUK12 Genome sequencing and assembly

PRJNA269542 • SRP105770 • All experiments • All runs

show Abstracthide Abstract

S.kebangsaanensis showed to have very good antibacterial and antifungal activity and also produce phenazine-1-carboxylic acid. Thus, the availability of the genome sequence of S.kebangsaanensis would provide an entry point to study the biosynthesis of phenazine and other antibiotics.

Sample:

SAMN03254380 • SRS2155050 • All experiments • All runs

Organism: Streptomyces kebangsaanensis

Library:

Name: CGATGT

Instrument: Illumina HiSeq 2000

Strategy: WGS

Source: GENOMIC

Selection: PCR

Layout: PAIRED

Runs: 1 run, 13.1M spots, 2.6G bases, 1.8Gb

Run	# of Spots	# of Bases	Size	Published
SRR5488792	13,112,394	2.6G	1.8Gb	2017-05-03

ID:: 3998991

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Sequence Read Archive

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