5MQ0: Structure of a spliceosome remodeled for exon ligation

PDB ID: 5MQ0Download
MMDB ID: 146601
PDB Deposition Date: 2016/12/19
Updated in MMDB: 2019/12
Experimental Method:
electron microscopy
Resolution: 4.17  Å
Source Organism:
Similar Structures:
Biological Unit for 5MQ0: 46-meric; determined by author
Molecular Components in 5MQ0
Label Count Molecule
Proteins (40 molecules)
1
Pre-mrna-splicing Factor 8
(Gene symbol: PRP8)
Molecule annotation
1
Pre-mrna-splicing Factor Snu114
(Gene symbol: SNU114)
Molecule annotation
1
Pre-mrna-splicing Factor Cwc22
(Gene symbol: CWC22)
Molecule annotation
1
Pre-mrna-splicing Factor Prp46
(Gene symbol: PRP46)
Molecule annotation
1
Pre-mrna-processing Protein 45
(Gene symbol: PRP45)
Molecule annotation
1
Pre-mrna-splicing Factor Bud31
(Gene symbol: BUD31)
Molecule annotation
1
Pre-mrna-splicing Factor Cwc2
(Gene symbol: CWC2)
Molecule annotation
1
Pre-mrna-splicing Factor Slt11
(Gene symbol: ECM2)
Molecule annotation
1
Pre-mrna-splicing Factor Cef1
(Gene symbol: CEF1)
Molecule annotation
1
Pre-mrna-splicing Factor Cwc15
(Gene symbol: CWC15)
Molecule annotation
1
Pre-mrna-splicing Factor Cwc21
(Gene symbol: CWC21)
Molecule annotation
1
Pre-mrna-splicing Factor Clf1
(Gene symbol: CLF1)
Molecule annotation
1
Pre-mrna-splicing Factor Syf1,pre-mrna-splicing Factor Syf1
(Gene symbol: SYF1)
Molecule annotation
1
Pre-mrna-splicing Factor 18
(Gene symbol: PRP18)
Molecule annotation
1
Pre-mrna-splicing Factor Slu7
(Gene symbol: SLU7)
Molecule annotation
1
Pre-mrna-processing Factor 17
(Gene symbol: CDC40)
Molecule annotation
1
Unknown Protein
Molecule annotation
1
Pre-mrna-splicing Factor Syf2
(Gene symbol: SYF2)
Molecule annotation
2
Small Nuclear Ribonucleoprotein-associated Protein B
(Gene symbol: SMB1)
Molecule annotation
2
Small Nuclear Ribonucleoprotein SM D3
(Gene symbol: SMD3)
Molecule annotation
2
Small Nuclear Ribonucleoprotein E
(Gene symbol: SME1)
Molecule annotation
2
Small Nuclear Ribonucleoprotein F
(Gene symbol: SMX3)
Molecule annotation
2
Small Nuclear Ribonucleoprotein G
(Gene symbol: SMX2)
Molecule annotation
2
Small Nuclear Ribonucleoprotein SM D1
(Gene symbol: SMD1)
Molecule annotation
2
Small Nuclear Ribonucleoprotein SM D2
(Gene symbol: SMD2)
Molecule annotation
1
Pre-mrna-splicing Factor Atp-dependent RNA Helicase Prp22
(Gene symbol: PRP22)
Molecule annotation
1
U2 Small Nuclear Ribonucleoprotein A'
(Gene symbol: LEA1)
Molecule annotation
1
U2 Small Nuclear Ribonucleoprotein B''
(Gene symbol: MSL1)
Molecule annotation
1
Pre-mrna-splicing Factor Snt309
(Gene symbol: SNT309)
Molecule annotation
4
Pre-mrna-processing Factor 19
(Gene symbol: PRP19)
Molecule annotation
Nucleotides(6 molecules)
1
Yeast Ubc4 Gene for Ubiquitin-conjugating Enzyme
Molecule annotation
1
5'-exon of Ubc4 Pre-mrna
Molecule annotation
1
S.cerevisiae Chromosome II Reading Frame ORF Ybr230c
Molecule annotation
1
Saccharomyces Cerevisiae Strain T.52_2h Chromosome XII Sequence
Molecule annotation
1
Saccharomyces Cerevisiae Strain Wi_c_mbsp_4 Chromosome VII Sequence
Molecule annotation
1
3'-exon of Ubc4 Pre-mrna, Bound by Prp22 Helicase
Molecule annotation
Chemicals and Non-standard biopolymers (13 molecules)
1
3
MAGNESIUM ION (Link to PubChem not yet available)MAGNESIUM ION
2
2
POTASSIUM ION (Link to PubChem not yet available)POTASSIUM ION
3
1
INOSITOL HEXAKISPHOSPHATE (Link to PubChem not yet available)INOSITOL HEXAKISPHOSPHATE
4
1
GUANOSINE-5'-TRIPHOSPHATE (Link to PubChem not yet available)GUANOSINE-5'-TRIPHOSPHATE
5
6
ZINC ION (Link to PubChem not yet available)ZINC ION
* Click molecule labels to explore molecular sequence information.
Citing MMDB
Madej T, Lanczycki CJ, Zhang D, Thiessen PA, Geer RC, Marchler-Bauer A, Bryant SH. " MMDB and VAST+: tracking structural similarities between macromolecular complexes. Nucleic Acids Res. 2014 Jan; 42(Database issue):D297-303