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8GZU: Cryo-EM structure of Tetrahymena thermophila respiratory Megacomplex MC (IV2+I+III2+II)2

PDB ID:	8GZU Download
MMDB ID:	231719
PDB Deposition Date:	2022/9/27
Updated in MMDB:	2024/10
Experimental Method:	electron microscopy
Resolution:	4.18 Å
Source Organism:	Tetrahymena thermophila SB210
Similar Structures:

Biological Unit for 8GZU: 436-meric; determined by author

Molecular Components in 8GZU
Label	Count	Molecule
Proteins (436 molecules)
00 55 a A	4	Transmembrane Protein, Putative
01 56 b B	4	Protein Phosphatase 2C, Putative
02 57 c C	4	Coxtt3
03 58 d D	4	Surf1-like Protein
04 59 e E	4	Trab Family Protein
05 60 f F	4	Transmembrane Protein, Putative
06 61 g G	4	Nadh Dehydrogenase [ubiquinone] 1 Alpha Subcomplex Subunit 8, Mitochondrial
07 62 h H	4	Surf1-like Protein
08 63 i I	4	Coxtt9
09 64 j J	4	Coxtt10
0A 45 6t 6T	4	Annexin
0B 46 bp BP	4	Chromosome Condensation Regulator Rcc1 Repeat Protein
0C 47 fs FS	4	Iron-binding Zinc Finger Cdgsh Type Protein
0D 48 4A 4a	4	Phage Protein
0E 49 y7 Y7	4	Ymf67
0F 50 y5 Y5	4	Ymf75
0G 51 y0 Y0	4	Ymf70
0H 52 z1 Z1	4	Coxtt28
0I 53 u1 U1	4	Unknown Peptide
0J 54 qM qm QM Qm u2 U2	8	Unknown Peptide
10 65 k K	4	39S Ribosomal Protein L9, Mitochondrial
11 66 l L	4	Ubiquinol-cytochrome C Reductase Complex Ubiquinone-binding Protein Qp-c
12 67 m M	4	Transmembrane Protein, Putative
13 68 n N	4	Transmembrane Protein, Putative
14 69 o O	4	Mobilization Protein
15 70 p P	4	Yflt Domain-containing Protein
16 71 q Q	4	Transmembrane Protein, Putative
17 72 r R	4	Transmembrane Protein
18 73 s S	4	Complex III Subunit VII
19 74 t T	4	Transmembrane Protein, Putative
1b 1B	2	Nadh Dehydrogenase Subunit 1
20 75 u U	4	Transmembrane Protein, Putative
21 76 v V	4	Coxtt22
22 77 w W	4	Transmembrane Protein, Putative
23 78 x X	4	Transmembrane Protein, Putative
24 79 y Y	4	Lysozyme
25 80 z Z	4	ABC Transporter
26 81 c1 C1	4	Cytochrome C Oxidase Subunit 1
27 82 c2 C2	4	Cytochrome C Oxidase Subunit 2
28 83 c3 C3	4	Ymf68
29 84 vb VB	4	Cytochrome C Oxidase Subunit VB Protein
2b 2B	2	Nadh Dehydrogenase Subunit 2
2e 2E	2	Nmra Domain-containing Protein
2f 2F	2	Transmembrane Protein, Putative
2g 2G	2	Sdhtt3
2h 2H	2	Diphthamide Synthesis Protein
2i 2I	2	Duf4885 Domain-containing Protein
2j 2J	2	Transmembrane Protein, Putative
2k 2K	2	Transmembrane Protein, Putative
2l 2L	2	Transposase
2m 2M	2	Transmembrane Protein, Putative
2n 2N	2	Transmembrane Protein, Putative
2o 2O	2	Sdhtt11
30 85 6a 6A	4	Transmembrane Protein, Putative
31 86 6b 6B	4	Structural Protein
32 87 6c 6C	4	Transmembrane Protein, Putative
33 88 6l 6L	4	Decapping Nuclease
34 89 7a 7A	4	Transmembrane Protein, Putative
35 90 7c 7C	4	Nadh Dehydrogenase [ubiquinone] 1 Beta Subcomplex Subunit 8, Mitochondrial
36 91 7l 7L	4	Ctf/nf-i Domain-containing Protein
37 92 m1 M1	4	Oxoglutarate/malate Translocator Protein, Putative
38 93 m2 M2	4	2-oxoglutarate/malate Carrier Protein
39 94 m3 M3	4	Carrier Protein
40 95 1t 1T	4	Tim10/ddp Family Zinc Finger Protein
41 96 2T 2t	4	Zf-tim10_ddp Domain-containing Protein
42 97 3T 3t	4	Zf-tim10_ddp Domain-containing Protein
43 98 4T 4t	4	Transposase
44 99 5t 5T	4	Cullin Domain-containing Protein
4l 4L	2	Ymf58
5b 5B	2	Ymf57
a1 A1	2	Transmembrane Protein, Putative
a2 A2	2	Ribosomal Protein L51/s25/ci-b8 Domain Protein
a3 A3	2	Transmembrane Protein, Putative
a5 A5	2	ETC Complex I Subunit Motif Protein
a6 A6	2	Nadh Dehydrogenase, Putative
a7 A7	2	37S Ribosomal Protein S25, Mitochondrial
a8 A8	2	Cx9c Domain-containing Protein
a9 A9	2	Nad-dependent Epimerase/dehydratase Family Protein
ab AB	2	Acyl Carrier Protein
ac AC	2	Acyl Carrier Protein
al AL	2	Nadh Dehydrogenase [ubiquinone] 1 Alpha Subcomplex Subunit 12
am AM	2	Ndua13
an AN	2	Transmembrane Protein, Putative
b2 B2	2	Ndub2
b3 B3	2	Transmembrane Protein, Putative
b4 B4	2	Ndub4
b6 B6	2	Ndub6
b7 B7	2	Chch Domain-containing Protein
b8 B8	2	Ndub8
b9 B9	2	Nadh Dehydrogenase [ubiquinone] 1 Alpha Subcomplex Subunit 10, Mitochondrial
bl BL	2	Ndub10
bm BM	2	Transmembrane Protein, Putative
c4 C4	2	Complex I-mnll
fx FX	2	2 Iron, 2 Sulfur Cluster-binding Protein
g1 G1	2	Gamma-carbonic Anhydrase
g2 G2	2	Gamma-carbonic Anhydrase
g3 G3	2	Transcription Factor Apfi Protein, Putative
j1 J1	2	Dnaj Domain Protein
n1 N1	2	Nadh-ubiquinone Oxidoreductase Chain 1
n2 N2	2	Ymf65
n3 N3	2	Nadh-ubiquinone Oxidoreductase Chain 3
n4 N4	2	Nadh-ubiquinone Oxidoreductase Chain 4
n5 N5	2	Nadh Dehydrogenase Subunit 5
n6 N6	2	Ymf62
p1 P1	2	Transmembrane Protein, Putative
p2 P2	2	Nduph2
qA qa QA Qa	4	M16 Family Peptidase, Putative
qB qb QB Qb	4	Peptidase M16 Inactive Domain Protein
qC qc QC Qc	4	Apocytochrome B
qD qd QD Qd	4	Cytochrome Protein C1
qE qe QE Qe	4	Rieske Iron-sulfur Protein, Ubiquinol-cytochrome C Reductase Iron-sulfur Subunit
qF qf QF Qf	4	Ubiquinol-cytochrome C Reductase Hinge Protein
qG qg QG Qg	4	Sulphotransf Domain-containing Protein
qH qh QH Qh	4	Transmembrane Protein, Putative
qI qi QI Qi	4	Transmembrane Protein, Putative
qJ qj QJ Qj	4	Transmembrane Protein, Putative
qL ql QL Ql	4	Uqcrtt2
s1 S1	2	Nadh-ubiquinone Oxidoreductase 75 KDA Subunit
s2 S2	2	Nadh Dehydrogenase Subunit 7
s3 S3	2	Nadh Dehydrogenase Subunit 9
s4 S4	2	Nadh Dehydrogenase [ubiquinone] Iron-sulfur Protein 4, Mitochondrial
s5 S5	2	Gram Domain Protein
s6 S6	2	Zinc-finger Protein
s7 S7	2	Nadh Dehydrogenase Subunit 10
s8 S8	2	Nadh-ubiquinone Oxidoreductase 1, Chain, Putative
sa SA	2	Succinate Dehydrogenase [ubiquinone] Flavoprotein Subunit, Mitochondrial
sb SB	2	Succinate Dehydrogenase (Quinone)
sc SC	2	Cytochrome B-c1 Complex Subunit 8
sd SD	2	Sdhd
t1 T1	2	Lipid-a-disaccharide Synthase
t2 T2	2	Acyl-coa Synthetase (Amp-forming)/amp-acid Ligase II
t3 T3	2	Rnase III Domain-containing Protein
t4 T4	2	Transmembrane Protein
t5 T5	2	Transmembrane Protein, Putative
t6 T6	2	COX Assembly Mitochondrial Protein
t7 T7	2	Transmembrane Protein, Putative
t8 T8	2	PH Domain-containing Protein
t9 T9	2	Nadh Dehydrogenase [ubiquinone] 1 Alpha Subcomplex Subunit 8
ta TA	2	Nadh Dehydrogenase [ubiquinone] 1 Beta Subcomplex Subunit 4
tb TB	2	Transmembrane Protein, Putative
tc TC	2	ATP Synthase Subunit E, Mitochondrial
td TD	2	Transmembrane Protein, Putative
te TE	2	Transmembrane Protein, Putative
tf TF	2	Ndutt15
tg TG	2	Ndutt16
th TH	2	Ndutt17
tx TX	2	Thioredoxin
v1 V1	2	Nadh Dehydrogenase [ubiquinone] Flavoprotein 1, Mitochondrial
v2 V2	2	Nadh-ubiquinone Oxidoreductase 24 KDA Subunit
x1 X1	2	Nadh-ubiquinone Oxidoreductase Complex I, 21 KDA Subunit
Chemicals and Non-standard biopolymers (98 molecules)
1	20	FE2/S2 (INORGANIC) CLUSTER
2	12	PROTOPORPHYRIN IX CONTAINING FE
3	8	HEME-A
4	12	COPPER (II) ION
5	4	MAGNESIUM ION
6	10	ZINC ION
7	6	HEME C
8	2	NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE
9	2	ADENOSINE-5'-DIPHOSPHATE
10	2	UBIQUINONE-10
11	14	IRON/SULFUR CLUSTER
12	2	FLAVIN-ADENINE DINUCLEOTIDE
13	2	FE3-S4 CLUSTER
14	2	FLAVIN MONONUCLEOTIDE