8TO1: Escherichia coli RNA polymerase unwinding intermediate (I1a) at the lambda PR promoter

PDB ID: 8TO1Download
MMDB ID: 249245
PDB Deposition Date: 2023/8/2
Updated in MMDB: 2024/07
Experimental Method:
electron microscopy
Resolution: 2.8  Å
Source Organism:
Escherichia phage Lambda▼
Similar Structures:
Biological Unit for 8TO1: nonameric; determined by author and by software
Molecular Components in 8TO1
Label Count Molecule
Proteins (7 molecules)
3
DNA-directed RNA Polymerase Subunit Alpha
Molecule annotation
1
DNA-directed RNA Polymerase Subunit Beta
Molecule annotation
1
DNA-directed RNA Polymerase Subunit Beta'
Molecule annotation
1
DNA-directed RNA Polymerase Subunit Omega
Molecule annotation
1
RNA Polymerase Sigma Factor Rpod
Molecule annotation
Nucleotides(2 molecules)
1
Nontemplate Strand of Lamdba PR Promoter DNA
Molecule annotation
1
Template Strand of Lamdba PR Promoter DNA
Molecule annotation
Chemicals and Non-standard biopolymers (7 molecules)
1
4
(3R,5S,7R,8R,9S,10S,12S,13R,14S,17R)-10,13-dimethyl-17-[(2R)-pentan-2-yl]-2,3,4,5,6,7,8,9,11,12,14,15,16,17-tetradecahydro-1H-cyclopenta[a]phenanthrene-3,7,12-triol(3R,5S,7R,8R,9S,10S,12S,13R,14S,17R)-10,13-dimethyl-17-[(2R)-pentan-2-yl]-2,3,4,5,6,7,8,9,11,12,14,15,16,17-tetradecahydro-1H-cyclopenta[a]phenanthrene-3,7,12-triol
2
1
MAGNESIUM IONMAGNESIUM ION
3
2
ZINC IONZINC ION
* Click molecule labels to explore molecular sequence information.
Citing MMDB
Madej T, Lanczycki CJ, Zhang D, Thiessen PA, Geer RC, Marchler-Bauer A, Bryant SH. " MMDB and VAST+: tracking structural similarities between macromolecular complexes. Nucleic Acids Res. 2014 Jan; 42(Database issue):D297-303