GEO DataSets

(Submitter supplied) Gene expression analysis of A. parasiticus grown under conditions conducive and nonconductive for aflatoxin production was evaluated using glass slide microarrays containing 765 ESTs. Aflatoxins (AF) produced by Aspergillus flavus and A. parasiticus are responsible for approximately $270M in losses annually in the corn, peanut, cotton, and tree nut industries of the United States. In an attempt to develop effective control procedures, research has focused on elucidating the pathway of aflatoxin biosynthesis with the goal of identifying potential targets for intervention. more...

Organism:

Aspergillus flavus; Aspergillus parasiticus

Type:

Expression profiling by array

Platforms:

GPL1393 GPL1394

16 Samples

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(Submitter supplied) The carcinogen aflatoxin is synthesized by a cluster of genes that are regulated by the transcriptional factor, AflR. Most, but not all of these genes, have a consensus binding site for AflR in their 5’ untranslated region. Because aflR resides within the biosynthetic cluster, is has been suggested that it regulates only genes within the cluster. The objective of this study was to identify those genes transcriptionally regulated by AflR and to determine if any of those genes reside outside the aflatoxin biosynthetic cluster. more...

Organism:

Aspergillus parasiticus; Aspergillus flavus

Type:

Expression profiling by array

Platform:

GPL2122

12 Samples

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(Submitter supplied) Aflatoxins are toxic and carcinogenic polyketide metabolites produced by fungal species, including Aspergillus flavus and A. parasiticus. The biosynthesis of aflatoxins is modulated by many environmental factors, including the availability of a carbon source. The gene expression profile of A. parasiticus was evaluated during a shift from a medium with low concentration of simple sugars, Yeast Extract (YE), to a similar medium with sucrose, Yeast Extract Sucrose (YES). more...

Organism:

Aspergillus parasiticus; Aspergillus flavus

Type:

Expression profiling by array

Platform:

GPL2122

22 Samples

Download data: TXT

(Submitter supplied) Aflatoxins are carcinogenic fungal secondary metabolites. Levels of aflatoxins in agricultural commodities are stringently regulated by many countries. A cluster of genes is responsible for aflatoxin biosynthesis by Aspergillus flavus and other closely related species. Expression of the clustered aflatoxin genes is governed by a complex network of regulatory mechanisms. To better understand the molecular events that are associated with aflatoxin production, transcription profiling by microarray analyses which compared three independent aflatoxigenic A. more...

Organism:

Aspergillus flavus

Type:

Expression profiling by array

Platform:

GPL2122

12 Samples

Download data: TXT

(Submitter supplied) Aflatoxins are toxic and carcinogenic secondary metabolites produced by the fungi Aspergillus flavus and A. parasiticus. In order to better understand the molecular mechanisms that regulate aflatoxin production, the biosynthesis of the toxin in A. flavus and A. parasticus grown in yeast extract sucrose media supplemented with 50 mM tryptophan (Trp) were examined. A. flavus grown in the presence of 50 mM tryptophan was found to have significantly reduced aflatoxin B1 and B2 biosynthesis, while A. more...

Organism:

Aspergillus parasiticus; Aspergillus flavus; Aspergillus flavus NRRL3357

Type:

Expression profiling by array

Platform:

GPL2122

12 Samples

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(Submitter supplied) Gene expression analysis of A. parasiticus grown under conditions conducive and nonconductive for aflatoxin production was evaluated using glass slide microarrays containing the 753 ESTs. A complex regulatory network governs the biosynthesis of aflatoxin. While several genes involved in aflatoxin production are known, their action alone cannot account for its regulation. Arrays of clones from an Aspergillus flavus cDNA library and glass slide microarrays of ESTs were screened to identify additional genes. more...

Organism:

Aspergillus flavus; Aspergillus parasiticus

Type:

Expression profiling by array

Platform:

GPL225

4 Samples

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(Submitter supplied) To better understand the effect of temperature on mycotoxin biosynthesis, RNA-Seq technology was used to profile the Aspergillus flavus transcriptome under different temperature conditions. This approachallowed us to quantify transcript abundance for over 80% of fungal genes including 1,153 genes that were differentially expressed at 30°C and 37°C. Wleven of the 55 secondary metabolite clusters were up-regulated at the lower temperature, including aflatoxin biosynthesis genes, which were among the most highly up-expressed genes. more...

Organism:

Aspergillus flavus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL13733

2 Samples

Download data: TXT

(Submitter supplied) We used cDNA microarray technology to compare the genome-wide expression profiles of a wild type strain (BY4700) (E02, E04, E06) or the isogenic strain deleted of GLN3 and GAT1 genes (E01, E03, E05) grown in YNB medium with glutamine as nitrogen source (M.Gln) against the wild type strain grown in M.Gln after addition of rapamacyn (20 min) (E01, E02), or M.proline (M.Pro) (E03, E04) or after a two hours shift from M.Gln to M.Pro (E05, E06), all growth conditions known to modify the expression of genes involved in nitrogen utilization. more...

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array

Platforms:

GPL2602 GPL2600 GPL2603

20 Samples

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(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Moniliophthora perniciosa

Type:

Expression profiling by array

Platforms:

GPL6149 GPL6148

16 Samples

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(Submitter supplied) Moniliophthora perniciosa is the causal agent of cacao Witche´s broom disease. This disease has been causing extensive damages to Brazilian cacao plantation, especially in Southern Bahia. Using glass slides microarrays, we analyzed the expression profile of 3872 whole genome shotgun reads from M. perniciosa genome, comparing two stages of development (Biotrophic-like mycelia and saprotrophic mycelia). more...

Organism:

Moniliophthora perniciosa

Type:

Expression profiling by array

Platform:

GPL6149

8 Samples

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(Submitter supplied) Moniliophthora perniciosa is the causal agent of cacao Witche´s broom disease. This disease has been causing extensive damages to Brazilian cacao plantation, especially in Southern Bahia. Using glass slides microarrays, we analyzed the expression profile of 3872 whole genome shotgun reads from M. perniciosa genome, comparing two stages of development (Biotrophic-like mycelia and saprotrophic mycelia). more...

Organism:

Moniliophthora perniciosa

Type:

Expression profiling by array

Platform:

GPL6148

8 Samples

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(Submitter supplied) This experiment was designed to analyze transcriptional changes that occur when the fungal plant pathogen M. grisea is shifted from a complete media to a nitrogen-starved media. Comparisons were made in the following way between fungal mycelial grown in minimal media supplemented with a nitrogen source (MM+N) and minimal media without a nitrogen source (NS) Keywords: parallel sample

Organism:

Pyricularia grisea

Type:

Expression profiling by array

Platform:

GPL889

6 Samples

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