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| Status |
Public on Jun 16, 2011 |
| Title |
Aspergillus flavus transcriptome under different temperature conditions |
| Organism |
Aspergillus flavus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
To better understand the effect of temperature on mycotoxin biosynthesis, RNA-Seq technology was used to profile the Aspergillus flavus transcriptome under different temperature conditions. This approachallowed us to quantify transcript abundance for over 80% of fungal genes including 1,153 genes that were differentially expressed at 30°C and 37°C. Wleven of the 55 secondary metabolite clusters were up-regulated at the lower temperature, including aflatoxin biosynthesis genes, which were among the most highly up-expressed genes. On average, transcript abundance for the 30 aflatoxin biosynthesis genes was 3,300 times greater at 30°C as compared to 37°C. The results are consistent with the view that high temperature negatively affects aflatoxin production by turning down transcription of the two key transcriptional regulators, aflR and aflS. Subtle changes in the expression levels of aflS to aflR appear to control transcription activation of the aflatoxin cluster.
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| Overall design |
2 samples examined: from the fungus grown at 30°C and 37°C
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| Contributor(s) |
Yu J, Fedorova ND, Montalbano BG, Bhatnagar D, Cleveland TE, Bennett JW, Nierman WC |
| Citation(s) |
21707733 |
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| Submission date |
Jun 16, 2011 |
| Last update date |
May 15, 2019 |
| Contact name |
Ravi Sanka |
| E-mail(s) |
[email protected]
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| Organization name |
JCVI
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| Street address |
9704 Medical Center Drive
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| City |
Rockville |
| State/province |
Maryland |
| ZIP/Postal code |
20850 |
| Country |
USA |
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| Platforms (1) |
| GPL13733 |
Illumina Genome Analyzer II (Aspergillus flavus) |
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| Samples (2) |
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| Relations |
| SRA |
SRP007212 |
| BioProject |
PRJNA144055 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE30031_normalized_data.txt.gz |
321.3 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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