 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Nov 28, 2024 |
| Title |
Widespread termination of mammalian RNA polymerase II at T-rich DNA sequences (POINT-Seq) |
| Organism |
Homo sapiens |
| Experiment type |
Other
|
| Summary |
The best-studied mechanism of eukaryotic RNA polymerase II (RNAPII) transcriptional termination is at protein-coding genes and involves endonucleolytic cleavage of the nascent RNA at the polyadenylation site. The RNAPII-associated cleavage product is then degraded 5'→3’ by XRN2 to elicit termination. In contrast, prokaryotic RNAP and eukaryotic RNAPIII often terminate directly over T-tracts in the non-template DNA strand. Here, we demonstrate a similar capability for mammalian RNAPII. This mechanism terminates snRNA transcription, which we unexpectedly show to be Integrator-independent. It is more generally employed where RNAPII elongation competence is low, especially in promoter-proximal regions and downstream of some protein-coding genes. In contrast, RNAPII within gene bodies does not terminate at T-tracts. Finally, XRN2-dependent, and T-tract termination are usually independent: the former acts following polyadenylation site cleavage, whereas the latter is employed where XRN2 cannot be engaged. Overall, we propose that RNAP’s retain the potential to terminate over T-rich sequences throughout evolution
|
| |
|
| Overall design |
Comparative meta and gene expression analysis of POINT-seq technology for HCT116 INTS1-AID degron cell lines (with or without KD)
|
| |
|
| Contributor(s) |
Davidson L, Rouvière JO, Sousa-Luís R, Nojima T, Proudfoot N, Heick Jensen T, West S |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Apr 23, 2024 |
| Last update date |
Nov 28, 2024 |
| Contact name |
Steven West |
| E-mail(s) |
[email protected]
|
| Phone |
0044(0)1392727458
|
| Organization name |
University of Exeter
|
| Department |
Living Systems Institute
|
| Street address |
Stocker Rd
|
| City |
Exeter |
| State/province |
Devon |
| ZIP/Postal code |
EX4 4QD |
| Country |
United Kingdom |
| |
|
| Platforms (1) |
| GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
|
| Samples (6)
|
|
| Relations |
| BioProject |
PRJNA1103842 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE264717_F_POINTseq_Control.bw |
1.1 Gb |
(ftp)(http) |
BW |
| GSE264717_F_POINTseq_INTS1KD.bw |
1.2 Gb |
(ftp)(http) |
BW |
| GSE264717_R_POINTseq_Control.bw |
1.1 Gb |
(ftp)(http) |
BW |
| GSE264717_R_POINTseq_INTS1KD.bw |
1.1 Gb |
(ftp)(http) |
BW |
SRA Run Selector |
| Raw data are available in SRA |
|
|
|
|
 |
Less...
More...