Data table header descriptions |
ID_REF |
|
VALUE |
Log2 ratio of normalized mean intensity of Cy5(N635_Mean) to normalized mean intensity of Cy3(N532_Mean) |
N635_Mean |
The normalized mean intensity of Cy5 calculated by subtracting the Cy5 mean background intensity taken from a standard area around the spot from the Cy5 emission in the pixels at each spot after laser excitation, and normalized to their overall mean, and then re-scaled to produce an overall geometric mean Cy5/Cy3 ratio of 1 for the array. |
N532_Mean |
The normalized mean intensity of Cy3 calculated by subtracting the Cy3 mean background intensity taken from a standard area around the spot from the Cy3 emission in the pixels at each spot after laser excitation, and normalized to their overall mean, and then re-scaled to produce an overall geometric mean Cy5/Cy3 ratio of 1 for the array. |
F635_Flag |
Flag data from Cy5 channel. The indication of spot quality determined by the image analysis software. Unflaged spot was assigned a value of zero. A negative value was assigned to a spot with intensity near background, uneven spot or background intensity. |
F635_Mean |
Cy5 channel average intensity of the spot. |
B635_Mean |
Cy5 channel background average intensity around the spot. |
F635_Median |
Cy5 channel median intensity of the spot. |
B635_Median |
Cy5 channel background median intensity around the spot. |
F635_SD |
The standard deviation calculated for the Cy5 channel mean intensity of the spot. |
B635_SD |
The standard deviation calculated for the Cy5 channel background average intensity around the spot. |
F532_Flag |
Flag data from Cy3 channel. The indication of spot quality determined by the image analysis software. Unflaged spot was assigned a value of zero. A negative value was assigned to a spot with intensity near background, uneven spot or background intensity. |
F532_Mean |
Cy3 channel average intensity of the spot. |
B532_Mean |
Cy3 channel background average intensity around the spot. |
F532_Median |
Cy3 channel median intensity of the spot. |
B532_Median |
Cy3 channel background median intensity around the spot. |
F532_SD |
The standard deviation calculated for the Cy3 channel mean intensity of the spot. |
B532_SD |
The standard deviation calculated for the Cy3 channel background average intensity around the spot. |