|
| Status |
Public on Jun 21, 2005 |
| Title |
Seguin_IDR_BrownNorway_Liver_control3 |
| Sample type |
RNA |
| |
|
| Source name |
Brown Norway Control Liver
|
| Organism |
Rattus norvegicus |
| Characteristics |
Organism: Rattus norvegicus
Strain: Brown Norway
Weight: 150-170 grams
Tissue: liver
Gender: male
Sacrifice Time: 6 hours
|
| Treatment protocol |
Rats were given water (vehicle, n = 4) or 1 mL of D-penicillamine
dissolved in tap water in a single dose of 150 mg/kg by oral
gavage (n = 8). Animals were sacrificed 6 hours after initial dosing.
|
| Growth protocol |
Male Brown Norway (BN) rats weighing 150-
170 g were purchased from Charles River (Montreal, QC). All
animals were housed in plastic cages (two/cage) with wooden
chips as bedding in a 12:12 h light:dark cycle at 22 °C. Rats
were given access to standard rat chow (Agribrands, Purina
Canada, Strathroy, ON) and ad libitum access to tap water for
1 week (acclimatization period) before starting experiments.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from frozen liver
tissues of individual rats using Qiagen RNeasy mini kits as
described by the manufacturer. RNA concentrations and purity
were determined spectrophotometrically. RNA quality was
further assessed by capillary electrophoresis using the Agilent
Bioanalyzer. We used 10 micrograms of total RNA from each individual
animal for microarray analysis.
|
| Label |
Affymetrix
|
| |
|
| Description |
Control sample #3
|
| Data processing |
RMA
|
| |
|
| Submission date |
Jun 18, 2005 |
| Last update date |
Jun 20, 2005 |
| Contact name |
Paul C Boutros |
| E-mail(s) |
[email protected]
|
| Organization name |
Ontario Institute for Cancer Research
|
| Street address |
101 College Street, Suite 800
|
| City |
Toronto |
| State/province |
Ontario |
| ZIP/Postal code |
M5G 0A3 |
| Country |
Canada |
| |
|
| Platform ID |
GPL341 |
| Series (1) |
| GSE2825 |
Profiling of D-PA Induced Idiosyncratic Drug Reactions |
|
