|
| Status |
Public on Nov 28, 2024 |
| Title |
1-Drd2 |
| Sample type |
SRA |
| |
|
| Source name |
Mus brain
|
| Organism |
Mus musculus |
| Characteristics |
Sex: male
tissue: Mus brain
cell type: neuron
genotype: Drd2-positive
|
| Treatment protocol |
The MM slices were prepared and digested in buffer that contained 10 mM Tris-Cl (pH 7.6), 50 mM NaF, 1 mM Na3VO4, 1 mM edetic acid, 1 mM benzamidine, 1 mM PMSF, 1 mg/10 ml papain, and a mixture of aprotinin, leupeptin, and pepstatin A (10 mg/ml each) for 30 min.Aspiration of fluorescent cells with glass electrodes
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was harvested using Rneasy mini plus kit (Qiagen). 1.3 ug of total RNA was used for the construction of sequencing libraries.
RNA libraries for RNA-seq were prepared using SMARTER mRNA-Seq Library Prep Kit following manufacturer's protocols.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Data processing |
Paired-end sequencing was performed on cDNA library using HiSeq 6000 instrument (Illumina).
The qualified reads were mapped to human reference genome hg38 (GRCh38) using STAR split mapping algorithm.
The expression level of genes was determined based on the value of FPKM
Assembly: mm10
Supplementary files format and content: tab-delimited text file include FPKM values for each Sample
|
| |
|
| Submission date |
Nov 28, 2023 |
| Last update date |
Nov 28, 2024 |
| Contact name |
Youming Lu |
| E-mail(s) |
[email protected]
|
| Organization name |
Huazhong University of Science and Technology
|
| Department |
School of Basic Medicine and Tongji Medical College
|
| Street address |
No. 13, Hangkong Road, Qiaokou District
|
| City |
Wuhan |
| State/province |
Hu Bei |
| ZIP/Postal code |
430000 |
| Country |
China |
| |
|
| Platform ID |
GPL24247 |
| Series (1) |
| GSE248796 |
Molecularly defined and functionally distinct subcircuits of the medial mammillary nucleus |
|
| Relations |
| BioSample |
SAMN38481080 |
| SRA |
SRX22671689 |
