|
| Status |
Public on Oct 23, 2024 |
| Title |
Loc1p IP (2) |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Loc1 tot. RNA (extract)
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
cell type: yeast cell
|
| Biomaterial provider |
Andre Gerber, Brown/Botstein labs, Stanford University School of Medicine
|
| Extracted molecule |
total RNA |
| Extraction protocol |
annotation not provided
|
| Label |
Cy3
|
| Label protocol |
N9/oligo dT primed, amino-allyl labeled.
|
| |
|
| Channel 2 |
| Source name |
Loc1 RNA-IP
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
cell type: yeast cell
|
| Biomaterial provider |
Andre Gerber, Brown/Botstein labs, Stanford University School of Medicine
|
| Extracted molecule |
total RNA |
| Extraction protocol |
annotation not provided
|
| Label |
Cy5
|
| Label protocol |
N9/oligo dT primed, amino-allyl labeled.
|
| |
|
| |
| Hybridization protocol |
annotation not provided
|
| Scan protocol |
annotation not provided
|
| Description |
ProtA Loc1 tagged cells were grown to OD=0.6. Loc1-zz immunoprecipitation and TEV elution. N9/oligo-dT and aminoallyl labeled.
|
| Data processing |
annotation not provided
|
| |
|
| Submission date |
Jul 29, 2024 |
| Last update date |
Oct 23, 2024 |
| Contact name |
André P. Gerber |
| E-mail(s) |
[email protected]
|
| Phone |
+44 (0)1483-688673
|
| Organization name |
University of Surrey
|
| Department |
Microbial Sciences
|
| Street address |
Stag Hill Campus
|
| City |
Guildford |
| State/province |
Surrey |
| ZIP/Postal code |
GU2 7XH |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL34753 |
| Series (1) |
|
| Data table header descriptions |
| ID_REF |
|
| VALUE |
Log (base 2) of the ratio of the mean of Channel 2 (usually 635 nm) to Channel 1 (usually 532 nm) [log (base 2) (RAT2N_MEAN)]. |
| RAT2N_MEAN |
Channel 2/Channel 1 ratio normalized, RAT2_MEAN/Normalization factor or Red/Green mean ratio normalized. |
| CH1D_MEAN |
Mean Channel 1 (usually 532 nm) intensity with the median-background subtracted (CH1I_MEAN - CH1B_MEDIAN) |
| CH2DN_MEAN |
Normalized value of mean Channel 2 (usually 635 nm) intensity with normalized background subtracted (CH2IN_MEAN - CH2BN_MEDIAN). |
| CH1I_MEAN |
Mean spot pixel intensity at Channel 1 (usually 532 nm). |
| CH1B_MEDIAN |
Median spot background intensity in Channel 1 (usually 532 nm). |
| CH2IN_MEAN |
Normalized value of mean Channel 2 (usually 635 nm) intensity (CH2I_MEAN/Normalization factor). |
| CH2BN_MEDIAN |
Normalized value of median Channel 2 (usually 635 nm) background (CH2B_MEDIAN/Normalization factor). |
| CORR |
Sqrt(Coefficient of determination for the current regression value) with sign inferred from sign of REGR column. Value of '-1' indicates an error in this field. |
| FLAG |
Type of flag associated with a spot. |
