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Conserved domains on  [gi|14278226|pdb|1IBX|A]
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Chain A, DNA FRAGMENTATION FACTOR 40

Protein Classification

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
CIDE_N super family cl02541
CIDE_N domain, found at the N-terminus of the CIDE (cell death-inducing DFF45-like effector) ...
 4-80 1.84e-43

CIDE_N domain, found at the N-terminus of the CIDE (cell death-inducing DFF45-like effector) proteins, as well as CAD nuclease (caspase-activated DNase/DNA fragmentation factor, DFF40) and its inhibitor, ICAD(DFF45). These proteins are associated with the chromatin condensation and DNA fragmentation events of apoptosis; the CIDE_N domain is thought to regulate the activity of ICAD/DFF45, and the CAD/DFF40 and CIDE nucleases during apoptosis. The CIDE-N domain is also found in the FSP27/CIDE-C protein.


The actual alignment was detected with superfamily member cd06535:

Pssm-ID: 470607  Cd Length: 77  Bit Score: 135.24  E-value: 1.84e-43
                       10        20        30        40        50        60        70
               ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
1IBX_A       4 KPKSVKLRALRSPRKFGVAGRSCQEVLRKGCLRFQLPERGSRLCLYEDGTELTEDYFPSVPDNAELVLLTLGQAWQG 80
Cdd:cd06535  1 KPKCVKIRSLNSAQKYGVAAKNLKELLRKGCRLLQLPCAGSRLCLYEDGTEVTEEYFPTLPDNTELVLLTPGQSWQG 77
 
Name Accession Description Interval E-value
CIDE_N_CAD cd06535
CIDE_N domain of CAD nuclease. The CIDE_N (cell death-inducing DFF45-like effector, N-terminal) ...
 4-80 1.84e-43

CIDE_N domain of CAD nuclease. The CIDE_N (cell death-inducing DFF45-like effector, N-terminal) domain is found at the N-terminus of CAD nuclease (caspase-activated DNase/DNA fragmentation factor, DFF40) and its inhibitor, ICAD(DFF45). These proteins are associated with the chromatin condensation and DNA fragmentation events of apoptosis; the CIDE_N domain is thought to regulate the activity of CAD/DFF40 and ICAD/DFF45 during apoptosis. In normal cells, DFF exists in the nucleus as a heterodimer composed of CAD/DFF40 as a latent nuclease and its chaperone and inhibitor subunit ICAD/DFF45. Apoptotic activation of caspase-3 results in the cleavage of DFF45/ICAD and the release of active DFF40/CAD nuclease.


Pssm-ID: 119368  Cd Length: 77  Bit Score: 135.24  E-value: 1.84e-43
                       10        20        30        40        50        60        70
               ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
1IBX_A       4 KPKSVKLRALRSPRKFGVAGRSCQEVLRKGCLRFQLPERGSRLCLYEDGTELTEDYFPSVPDNAELVLLTLGQAWQG 80
Cdd:cd06535  1 KPKCVKIRSLNSAQKYGVAAKNLKELLRKGCRLLQLPCAGSRLCLYEDGTEVTEEYFPTLPDNTELVLLTPGQSWQG 77
CIDE-N pfam02017
CIDE-N domain; This domain is found in CAD nuclease, and ICAD, the inhibitor of CAD nuclease. ...
 6-79 1.01e-25

CIDE-N domain; This domain is found in CAD nuclease, and ICAD, the inhibitor of CAD nuclease. The two proteins interact through this domain.


Pssm-ID: 460413  Cd Length: 75  Bit Score: 90.25  E-value: 1.01e-25
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
1IBX_A         6 KSVKLRALRSPRKFGVAGRSCQEVLRKGCLRFQLPERGSRLCLYEDGTEL-TEDYFPSVPDNAELVLLTLGQAWQ 79
Cdd:pfam02017  1 KPFKVCNVDRSRKKGVAASSLEELLQKGCEKLLLPSEPVTLVLEEDGTEVdDEEYFQTLPDNTELMLLEKGEKWT 75
CAD smart00266
Domains present in proteins implicated in post-mortem DNA fragmentation;
6-78 1.79e-22

Domains present in proteins implicated in post-mortem DNA fragmentation;


Pssm-ID: 128562  Cd Length: 74  Bit Score: 81.99  E-value: 1.79e-22
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
1IBX_A          6 KSVKLRALRSPRKFGVAGRSCQEVLRKGCLRFQLPERGSRLCLYEDGTEL-TEDYFPSVPDNAELVLLTLGQAW 78
Cdd:smart00266  1 RPFKVRDHDRNVRKGVAASSLEELLSKVCDKLALPDSPVTLVLEEDGTIVdDEEYFQTLPDNTELMALEKGEKW 74
 
Name Accession Description Interval E-value
CIDE_N_CAD cd06535
CIDE_N domain of CAD nuclease. The CIDE_N (cell death-inducing DFF45-like effector, N-terminal) ...
 4-80 1.84e-43

CIDE_N domain of CAD nuclease. The CIDE_N (cell death-inducing DFF45-like effector, N-terminal) domain is found at the N-terminus of CAD nuclease (caspase-activated DNase/DNA fragmentation factor, DFF40) and its inhibitor, ICAD(DFF45). These proteins are associated with the chromatin condensation and DNA fragmentation events of apoptosis; the CIDE_N domain is thought to regulate the activity of CAD/DFF40 and ICAD/DFF45 during apoptosis. In normal cells, DFF exists in the nucleus as a heterodimer composed of CAD/DFF40 as a latent nuclease and its chaperone and inhibitor subunit ICAD/DFF45. Apoptotic activation of caspase-3 results in the cleavage of DFF45/ICAD and the release of active DFF40/CAD nuclease.


Pssm-ID: 119368  Cd Length: 77  Bit Score: 135.24  E-value: 1.84e-43
                       10        20        30        40        50        60        70
               ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
1IBX_A       4 KPKSVKLRALRSPRKFGVAGRSCQEVLRKGCLRFQLPERGSRLCLYEDGTELTEDYFPSVPDNAELVLLTLGQAWQG 80
Cdd:cd06535  1 KPKCVKIRSLNSAQKYGVAAKNLKELLRKGCRLLQLPCAGSRLCLYEDGTEVTEEYFPTLPDNTELVLLTPGQSWQG 77
CIDE_N cd01615
CIDE_N domain, found at the N-terminus of the CIDE (cell death-inducing DFF45-like effector) ...
 4-80 1.03e-28

CIDE_N domain, found at the N-terminus of the CIDE (cell death-inducing DFF45-like effector) proteins, as well as CAD nuclease (caspase-activated DNase/DNA fragmentation factor, DFF40) and its inhibitor, ICAD(DFF45). These proteins are associated with the chromatin condensation and DNA fragmentation events of apoptosis; the CIDE_N domain is thought to regulate the activity of ICAD/DFF45, and the CAD/DFF40 and CIDE nucleases during apoptosis. The CIDE-N domain is also found in the FSP27/CIDE-C protein.


Pssm-ID: 119367  Cd Length: 78  Bit Score: 97.99  E-value: 1.03e-28
                       10        20        30        40        50        60        70
               ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
1IBX_A       4 KPKSVKLRALRSPRKFGVAGRSCQEVLRKGCLRFQLPERGSRLCLYEDGTEL-TEDYFPSVPDNAELVLLTLGQAWQG 80
Cdd:cd01615  1 PLRPFKVCDSDRSRKKGVAASSLEELLSKACEKLKLPSAPVTLVLEEDGTEVdDEEYFQTLPDNTVLMLLEPGQKWTP 78
CIDE-N pfam02017
CIDE-N domain; This domain is found in CAD nuclease, and ICAD, the inhibitor of CAD nuclease. ...
 6-79 1.01e-25

CIDE-N domain; This domain is found in CAD nuclease, and ICAD, the inhibitor of CAD nuclease. The two proteins interact through this domain.


Pssm-ID: 460413  Cd Length: 75  Bit Score: 90.25  E-value: 1.01e-25
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
1IBX_A         6 KSVKLRALRSPRKFGVAGRSCQEVLRKGCLRFQLPERGSRLCLYEDGTEL-TEDYFPSVPDNAELVLLTLGQAWQ 79
Cdd:pfam02017  1 KPFKVCNVDRSRKKGVAASSLEELLQKGCEKLLLPSEPVTLVLEEDGTEVdDEEYFQTLPDNTELMLLEKGEKWT 75
CAD smart00266
Domains present in proteins implicated in post-mortem DNA fragmentation;
6-78 1.79e-22

Domains present in proteins implicated in post-mortem DNA fragmentation;


Pssm-ID: 128562  Cd Length: 74  Bit Score: 81.99  E-value: 1.79e-22
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
1IBX_A          6 KSVKLRALRSPRKFGVAGRSCQEVLRKGCLRFQLPERGSRLCLYEDGTEL-TEDYFPSVPDNAELVLLTLGQAW 78
Cdd:smart00266  1 RPFKVRDHDRNVRKGVAASSLEELLSKVCDKLALPDSPVTLVLEEDGTIVdDEEYFQTLPDNTELMALEKGEKW 74
CIDE_N_A cd06539
CIDE_N domain of CIDE-A proteins. The CIDE_N (cell death-inducing DFF45-like effector, ...
 14-78 3.25e-08

CIDE_N domain of CIDE-A proteins. The CIDE_N (cell death-inducing DFF45-like effector, N-terminal) domain is found at the N-terminus of the CIDE (cell death-inducing DFF45-like effector) proteins. These proteins are associated with the chromatin condensation and DNA fragmentation events of apoptosis; the CIDE_N domain is thought to regulate the activity of the CAD/DFF40, ICAD/DFF45, and CIDE nucleases during apoptosis. The CIDE protein family includes 3 members: CIDE-A, CIDE-B, and FSP27(CIDE-C). Based on sequence similarity with DFF40 and DFF45, the CIDE proteins were initially characterized as mitochondrial activators of apoptosis. However, strong metabolic phenotypes of mice lacking CIDE-A and CIDE-B indicated that this family may play critical roles in energy balance.


Pssm-ID: 119372  Cd Length: 78  Bit Score: 46.09  E-value: 3.25e-08
                       10        20        30        40        50        60
               ....*....|....*....|....*....|....*....|....*....|....*....|....*.
1IBX_A      14 RSPRKfGVAGRSCQEVLRKGCLRFQLPERGSRLCLYEDGTEL-TEDYFPSVPDNAELVLLTLGQAW 78
Cdd:cd06539 12 RSSRR-GVMASSLQELISKTLDALVITSGLVTLVLEEDGTVVdTEEFFQTLGDNTHFMVLEKGQKW 76
CIDE_N_ICAD cd06536
CIDE_N domain of ICAD. The CIDE_N (cell death-inducing DFF45-like effector, N-terminal) ...
 6-80 2.60e-07

CIDE_N domain of ICAD. The CIDE_N (cell death-inducing DFF45-like effector, N-terminal) domain is found at the N-terminus of the CAD nuclease (caspase-activated DNase/DNA fragmentation factor, DFF40) and its inhibitor, ICAD (DFF45). These proteins are associated with the chromatin condensation and DNA fragmentation events of apoptosis; the CIDE_N domain is thought to regulate the activity of the CAD/DFF40 and ICAD/DFF45 during apoptosis. In normal cells, DFF exists in the nucleus as a heterodimer composed of CAD/DFF40 as a latent nuclease and its chaperone and inhibitor subunit ICAD/DFF45. Apoptotic activation of caspase-3 results in the cleavage of DFF45/ICAD and release of active DFF40/CAD nuclease.


Pssm-ID: 119369  Cd Length: 80  Bit Score: 43.63  E-value: 2.60e-07
                       10        20        30        40        50        60        70
               ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
1IBX_A       6 KSVKLRALRSPRKFGVAGRSCQEVLRKGC--LRFQLPERGSRLCLYEDGTELT-EDYFPSVPDNAELVLLTLGQAWQG 80
Cdd:cd06536  3 KPCVVCNVSRQKQHGVAASSLEELRIKACesLGFDSSSAPITLVLAEDGTIVEdEDYFLCLPPNTKFVLLAENEKWAP 80
CIDE_N_FSP27 cd06538
CIDE_N domain of FSP27 proteins. The CIDE-N (cell death-inducing DFF45-like effector, ...
 14-79 3.22e-06

CIDE_N domain of FSP27 proteins. The CIDE-N (cell death-inducing DFF45-like effector, N-terminal) domain is found in the FSP27/CIDE-C protein, which has been identified as a n adipocyte lipid droplet protein that negatively regulates lipolysis and promotes triglyceride accumulation. The CIDE protein family includes 3 members: CIDE-A, CIDE-B, and FSP27(CIDE-C). Based on sequence similarity with DFF40 and DFF45, CIDE proteins were initially characterized as mitochondrial activators of apoptosis. The CIDE-N domain of FSP27 is sufficient to increase apoptosis in vitro when overexpressed.


Pssm-ID: 119371  Cd Length: 79  Bit Score: 40.95  E-value: 3.22e-06
                       10        20        30        40        50        60
               ....*....|....*....|....*....|....*....|....*....|....*....|....*..
1IBX_A      14 RSPRKfGVAGRSCQEVLRKGCLRFQLPErGSRLCLYEDGTEL-TEDYFPSVPDNAELVLLTLGQAWQ 79
Cdd:cd06538 12 RSLRK-GIMADSLEDLLNKVLDALLLDC-ISSLVLDEDGTGVdTEEFFQALADNTVFMVLGKGQKWK 76
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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