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Conserved domains on  [gi|157833592|pdb|1PYC|A]
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Chain A, CYP1

Protein Classification

GAL4-like domain-containing transcription factor( domain architecture ID 10637819)

GAL4-like domain-containing transcription factor with an an N-terminal GAL4-like C6 zinc binuclear cluster DNA-binding domain

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
GAL4 smart00066
GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain; Gal4 is a positive ...
 4-44 1.64e-06

GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain; Gal4 is a positive regulator for the gene expression of the galactose- induced genes of S. cerevisiae. Is present only in fungi.


:

Pssm-ID: 214501 [Multi-domain]  Cd Length: 43  Bit Score: 40.42  E-value: 1.64e-06
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|.
1PYC_A          4 RIPLSCTICRKRKVKCDKLRPHCQQCTKTGVahLCHYMEQT 44
Cdd:smart00066  2 RVSKACDRCRKRKIKCDGKKPPCSNCIKLGL--ECTYSDSK 40
ZIP_Gal4p-like super family cl22421
Leucine zipper Dimerization domain of Gal4p-like transcription factors; The Gal4p family of ...
 44-71 8.27e-03

Leucine zipper Dimerization domain of Gal4p-like transcription factors; The Gal4p family of transcriptional activators contain an N-terminal DNA-binding domain with a Zn2Cys6 binuclear cluster that interact with CCG triplets and a leucine zipper-like heptad repeat that dimerizes. Dimerization allows binding of targets which contain two CCG motifs oriented in an inverted (CGG-CCG), direct (CCG-CCG), or everted (CCG-CGG) manner. Included in this family are Saccharomyces cerevisiae Gal4p, Hap1p, Put3p, Ppr1p and Sip4p, Neurospora crassa acu-15, and Colletotrichum acutatum Nir1, among others. Gal4p functions in the induction of GAL genes in the presence of galactose; GAL proteins are responsible for the transport of galactose into the cell and for its metabolism through the glycolytic pathway. Hap1p promotes transcription of genes required for respiration and controlling oxidative damage in response to heme. Put3p activates the transcription of the PUT1 and PUT2 genes in the presence of proline, allowing yeast cells to use proline as a nitrogen source. Ppr1p activates transcription of the URA1, URA3, and URA4 genes, which encode enzymes involved in the regulation of pyrimidine levels. Sip4p activates target genes under conditions of glucose deprivation. Acu-15 is involved in regulating acetate utilization while Nir1 plays a role during nitrogen-starvation conditions.


The actual alignment was detected with superfamily member cd14655:

Pssm-ID: 277561  Cd Length: 32  Bit Score: 30.80  E-value: 8.27e-03
                       10        20
               ....*....|....*....|....*...
1PYC_A      44 TWAEEAEKELLKDNELKKLRERVKSLEK 71
Cdd:cd14655  1 PWIEPVMKELAKGEEIKSLREEVKSLQK 28
 
Name Accession Description Interval E-value
GAL4 smart00066
GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain; Gal4 is a positive ...
 4-44 1.64e-06

GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain; Gal4 is a positive regulator for the gene expression of the galactose- induced genes of S. cerevisiae. Is present only in fungi.


Pssm-ID: 214501 [Multi-domain]  Cd Length: 43  Bit Score: 40.42  E-value: 1.64e-06
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|.
1PYC_A          4 RIPLSCTICRKRKVKCDKLRPHCQQCTKTGVahLCHYMEQT 44
Cdd:smart00066  2 RVSKACDRCRKRKIKCDGKKPPCSNCIKLGL--ECTYSDSK 40
GAL4 cd00067
GAL4-like Zn2Cys6 binuclear cluster DNA-binding domain; found in transcription regulators like ...
 4-40 4.91e-06

GAL4-like Zn2Cys6 binuclear cluster DNA-binding domain; found in transcription regulators like GAL4. Domain consists of two helices organized around a Zn(2)Cys(6 )motif; Binds to sequences containing 2 DNA half sites comprised of 3-5 C/G combinations


Pssm-ID: 238023 [Multi-domain]  Cd Length: 36  Bit Score: 38.80  E-value: 4.91e-06
                       10        20        30
               ....*....|....*....|....*....|....*..
1PYC_A       4 RIPLSCTICRKRKVKCDKLRPHCQQCTKTGvaHLCHY 40
Cdd:cd00067  1 RVRRACDRCRRRKIKCDGEKPPCSNCIKLG--LECTY 35
Zn_clus pfam00172
Fungal Zn(2)-Cys(6) binuclear cluster domain;
8-34 1.25e-04

Fungal Zn(2)-Cys(6) binuclear cluster domain;


Pssm-ID: 395120 [Multi-domain]  Cd Length: 39  Bit Score: 35.35  E-value: 1.25e-04
                         10        20
                 ....*....|....*....|....*..
1PYC_A         8 SCTICRKRKVKCDKLRPHCQQCTKTGV 34
Cdd:pfam00172  2 ACDNCRKRKVKCDGKKPACSRCIKNGL 28
ZIP_Hap1 cd14655
Leucine zipper Dimerization domain of transcription factor Hap1 and similar fungal proteins; ...
 44-71 8.27e-03

Leucine zipper Dimerization domain of transcription factor Hap1 and similar fungal proteins; Hap1p mediates oxygen sensing and heme signaling in yeast. In response to heme, it promotes transcription of genes required for respiration and controlling oxidative damage. It is a member of the Gal4/Gal4p family of transcriptional activators which contain an N-terminal DNA binding domain with a Zn2Cys6 binuclear cluster that interact with CCG triplets and a leucine zipper-like heptad repeat that dimerizes. Dimerization allows binding of targets which contain two CCG motifs oriented in an inverted (CGG-CCG), direct (CCG-CCG), or everted (CCG-CGG) manner. Hap1p binds to DNA containing a direct repeat of two CGG triplets. It is a large protein that contains repression modules (RPMs) and heme-responsive motifs (HRMs) in addition to the DNA-binding and dimerization domains.


Pssm-ID: 271239  Cd Length: 32  Bit Score: 30.80  E-value: 8.27e-03
                       10        20
               ....*....|....*....|....*...
1PYC_A      44 TWAEEAEKELLKDNELKKLRERVKSLEK 71
Cdd:cd14655  1 PWIEPVMKELAKGEEIKSLREEVKSLQK 28
 
Name Accession Description Interval E-value
GAL4 smart00066
GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain; Gal4 is a positive ...
 4-44 1.64e-06

GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain; Gal4 is a positive regulator for the gene expression of the galactose- induced genes of S. cerevisiae. Is present only in fungi.


Pssm-ID: 214501 [Multi-domain]  Cd Length: 43  Bit Score: 40.42  E-value: 1.64e-06
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|.
1PYC_A          4 RIPLSCTICRKRKVKCDKLRPHCQQCTKTGVahLCHYMEQT 44
Cdd:smart00066  2 RVSKACDRCRKRKIKCDGKKPPCSNCIKLGL--ECTYSDSK 40
GAL4 cd00067
GAL4-like Zn2Cys6 binuclear cluster DNA-binding domain; found in transcription regulators like ...
 4-40 4.91e-06

GAL4-like Zn2Cys6 binuclear cluster DNA-binding domain; found in transcription regulators like GAL4. Domain consists of two helices organized around a Zn(2)Cys(6 )motif; Binds to sequences containing 2 DNA half sites comprised of 3-5 C/G combinations


Pssm-ID: 238023 [Multi-domain]  Cd Length: 36  Bit Score: 38.80  E-value: 4.91e-06
                       10        20        30
               ....*....|....*....|....*....|....*..
1PYC_A       4 RIPLSCTICRKRKVKCDKLRPHCQQCTKTGvaHLCHY 40
Cdd:cd00067  1 RVRRACDRCRRRKIKCDGEKPPCSNCIKLG--LECTY 35
Zn_clus pfam00172
Fungal Zn(2)-Cys(6) binuclear cluster domain;
8-34 1.25e-04

Fungal Zn(2)-Cys(6) binuclear cluster domain;


Pssm-ID: 395120 [Multi-domain]  Cd Length: 39  Bit Score: 35.35  E-value: 1.25e-04
                         10        20
                 ....*....|....*....|....*..
1PYC_A         8 SCTICRKRKVKCDKLRPHCQQCTKTGV 34
Cdd:pfam00172  2 ACDNCRKRKVKCDGKKPACSRCIKNGL 28
ZIP_Hap1 cd14655
Leucine zipper Dimerization domain of transcription factor Hap1 and similar fungal proteins; ...
 44-71 8.27e-03

Leucine zipper Dimerization domain of transcription factor Hap1 and similar fungal proteins; Hap1p mediates oxygen sensing and heme signaling in yeast. In response to heme, it promotes transcription of genes required for respiration and controlling oxidative damage. It is a member of the Gal4/Gal4p family of transcriptional activators which contain an N-terminal DNA binding domain with a Zn2Cys6 binuclear cluster that interact with CCG triplets and a leucine zipper-like heptad repeat that dimerizes. Dimerization allows binding of targets which contain two CCG motifs oriented in an inverted (CGG-CCG), direct (CCG-CCG), or everted (CCG-CGG) manner. Hap1p binds to DNA containing a direct repeat of two CGG triplets. It is a large protein that contains repression modules (RPMs) and heme-responsive motifs (HRMs) in addition to the DNA-binding and dimerization domains.


Pssm-ID: 271239  Cd Length: 32  Bit Score: 30.80  E-value: 8.27e-03
                       10        20
               ....*....|....*....|....*...
1PYC_A      44 TWAEEAEKELLKDNELKKLRERVKSLEK 71
Cdd:cd14655  1 PWIEPVMKELAKGEEIKSLREEVKSLQK 28
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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