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Conserved domains on  [gi|166007380|pdb|3BUU|B]
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Chain B, Uncharacterized LolA superfamily protein NE2245

Protein Classification

outer membrane lipoprotein-sorting protein( domain architecture ID 12181914)

outer membrane lipoprotein-sorting protein similar to periplasmic molecular chaperone LolA that accepts outer membrane (OM)-specific lipoproteins that are released from the inner membrane by the LolCDE complex and transfers them to the OM receptor LolB

Gene Ontology:  GO:0009279|GO:0042953
PubMed:  15221203
SCOP:  3001964

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
LolA_like pfam17131
Outer membrane lipoprotein-sorting protein; This is likely to be a family of outer-membrane ...
 52-227 3.16e-58

Outer membrane lipoprotein-sorting protein; This is likely to be a family of outer-membrane lipoprotein-sorting proteins.


:

Pssm-ID: 435738 [Multi-domain]  Cd Length: 184  Bit Score: 181.99  E-value: 3.16e-58
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
3BUU_B         52 GNENSIV*ITEPASERGQAIL----*KGRDLWVF*PSVSQPIRLS---LSQRLTGQV-ANGDIARANFTGDYHPQLLRNE 123
Cdd:pfam17131   1 GDDKSLIVFLSPADVKGTAFLtldyGGDDDQWLYLPALKRVRRISssdKSQSFMGSDfSYEDLSRSRLVDDYTHELLGEE 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
3BUU_B        124 SIDDEDYYVLELTGIDRSVTYQKVLLWVNQSNFRPYKAEFYSVSGRLLKTSRYENFDNILGE*RPTRII*EDaLKSGEVS 203
Cdd:pfam17131  81 TVGGKDCYVLELTPKDKDVSYSKRVLWVDKETYLPLKAEFYDKSGKLLKTLTVSDIEKIDGRWTPTKMEMED-LQTGHKT 159

                         170       180
                  ....*....|....*....|....*
3BUU_B        204 VLDYSD*KLRD-LPDKIFTKDYLKR 227
Cdd:pfam17131 160 VLEISDIEFDTgLPDKLFTKRYLER 184
 
Name Accession Description Interval E-value
LolA_like pfam17131
Outer membrane lipoprotein-sorting protein; This is likely to be a family of outer-membrane ...
 52-227 3.16e-58

Outer membrane lipoprotein-sorting protein; This is likely to be a family of outer-membrane lipoprotein-sorting proteins.


Pssm-ID: 435738 [Multi-domain]  Cd Length: 184  Bit Score: 181.99  E-value: 3.16e-58
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
3BUU_B         52 GNENSIV*ITEPASERGQAIL----*KGRDLWVF*PSVSQPIRLS---LSQRLTGQV-ANGDIARANFTGDYHPQLLRNE 123
Cdd:pfam17131   1 GDDKSLIVFLSPADVKGTAFLtldyGGDDDQWLYLPALKRVRRISssdKSQSFMGSDfSYEDLSRSRLVDDYTHELLGEE 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
3BUU_B        124 SIDDEDYYVLELTGIDRSVTYQKVLLWVNQSNFRPYKAEFYSVSGRLLKTSRYENFDNILGE*RPTRII*EDaLKSGEVS 203
Cdd:pfam17131  81 TVGGKDCYVLELTPKDKDVSYSKRVLWVDKETYLPLKAEFYDKSGKLLKTLTVSDIEKIDGRWTPTKMEMED-LQTGHKT 159

                         170       180
                  ....*....|....*....|....*
3BUU_B        204 VLDYSD*KLRD-LPDKIFTKDYLKR 227
Cdd:pfam17131 160 VLEISDIEFDTgLPDKLFTKRYLER 184
LolA_like cd16329
proteins similar to periplasmic molecular chaperone LolA, the outer membrane lipoprotein ...
 8-221 1.09e-49

proteins similar to periplasmic molecular chaperone LolA, the outer membrane lipoprotein receptor LolB and the periplasmic protein RseB; This family contains uncharacterized proteins similar to the periplasmic molecular chaperone LolA, the outer membrane lipoprotein receptor LolB and the periplasmic protein RseB, all of which have similar unclosed beta-barrel structures that resemble a baseball glove-like scaffold consisting of an 11-stranded antiparallel sheet. There are five Lol proteins (LolA, LolB, LolC, LolD, and LolE) involved in the sorting and membrane localization of lipoprotein and are highly conserved in Gram-negative bacteria. LolA accepts outer membrane (OM)-specific lipoproteins that are released from the inner membrane by the LolCDE complex and transfers them to the OM receptor LolB. It is proposed that the LolA/LolB complex forms a tunnel-like structure, where the hydrophobic insides of LolA and LolB are connected, which enables lipoproteins to transfer from LolA to LolB. RseB exerts a crucial role in modulating the stability of RseA, the transmembrane anti-sigma-factor that is degraded during sigma-E-dependent transcription caused by bacterial envelope stress. Its structural similarity to LolA and LolB suggests that RseA may act as a sensor of periplasmic stress with a dual functionality, detecting mislocalized lipoproteins as well as propagating the signal to induce the sigma-E-response.


Pssm-ID: 319986  Cd Length: 225  Bit Score: 161.71  E-value: 1.09e-49
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
3BUU_B        8 TAQSILEKADEIRFPQDSFQVNVAIRTAaPDHAEDLYRYQVLSKG--NENSIV*ITEPASERGQAIL-----*KGRDLWV 80
Cdd:cd16329   1 SAEEILEKVDDRRRGDSSADVSTMTLTD-KNGKERTRELRVYSKDggDDKSLIRFLSPADVKGTAFLtldydDKDDDQWL 79

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
3BUU_B       81 F*PSVSQPIRLSLSQR----LTGQVANGDIARANFTGDYHPQLLRNESIDDEDYYVLELTGID-RSVTYQKVLLWVNQSN 155
Cdd:cd16329  80 YLPALRRVRRISSSDKsqsfMGSDFSYEDLSRSRLLEDYTYKLLGEEEVDGRDCYVLELTPKDgADSGYSKRVLWVDKDT 159

                       170       180       190       200       210       220
                ....*....|....*....|....*....|....*....|....*....|....*....|....*..
3BUU_B      156 FRPYKAEFYSVSGRLLKTSRYENFDNILGE*RPTRII*EDaLKSGEVSVLDYSD*KLR-DLPDKIFT 221
Cdd:cd16329 160 FLPLKAEYYDRSGKLLKTLTFSDIKKIGGYWRPTRMEMKD-LLTGHKTVLEYSDIKFNvGLPDSLFT 225
LolA COG2834
Outer membrane lipoprotein-sorting protein [Cell wall/membrane/envelope biogenesis];
60-182 1.91e-06

Outer membrane lipoprotein-sorting protein [Cell wall/membrane/envelope biogenesis];


Pssm-ID: 442082  Cd Length: 211  Bit Score: 47.00  E-value: 1.91e-06
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
3BUU_B       60 ITEPAserGQAIL*KGRDLWVF*PSVSQPIRLSLSQRLTGQVANGDiaRANFTGDYHPQLLRNEsiDDEDYYVLELTGID 139
Cdd:COG2834  76 YTKPY---EQLIVSDGKTVWIYDPDLNQVTVIPLSDATPLALLLGD--FSDLLKDFTVTLLGEE--TGRKAYVLELTPKD 148

                        90       100       110       120
                ....*....|....*....|....*....|....*....|...
3BUU_B      140 RSVTYQKVLLWVNQSNFrPYKAEFYSVSGrllKTSRYEnFDNI 182
Cdd:COG2834 149 KDSGFGKITLWFDKETL-LRKLEIYDADG---QRTTIT-FSNV 186
 
Name Accession Description Interval E-value
LolA_like pfam17131
Outer membrane lipoprotein-sorting protein; This is likely to be a family of outer-membrane ...
 52-227 3.16e-58

Outer membrane lipoprotein-sorting protein; This is likely to be a family of outer-membrane lipoprotein-sorting proteins.


Pssm-ID: 435738 [Multi-domain]  Cd Length: 184  Bit Score: 181.99  E-value: 3.16e-58
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
3BUU_B         52 GNENSIV*ITEPASERGQAIL----*KGRDLWVF*PSVSQPIRLS---LSQRLTGQV-ANGDIARANFTGDYHPQLLRNE 123
Cdd:pfam17131   1 GDDKSLIVFLSPADVKGTAFLtldyGGDDDQWLYLPALKRVRRISssdKSQSFMGSDfSYEDLSRSRLVDDYTHELLGEE 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
3BUU_B        124 SIDDEDYYVLELTGIDRSVTYQKVLLWVNQSNFRPYKAEFYSVSGRLLKTSRYENFDNILGE*RPTRII*EDaLKSGEVS 203
Cdd:pfam17131  81 TVGGKDCYVLELTPKDKDVSYSKRVLWVDKETYLPLKAEFYDKSGKLLKTLTVSDIEKIDGRWTPTKMEMED-LQTGHKT 159

                         170       180
                  ....*....|....*....|....*
3BUU_B        204 VLDYSD*KLRD-LPDKIFTKDYLKR 227
Cdd:pfam17131 160 VLEISDIEFDTgLPDKLFTKRYLER 184
LolA_like cd16329
proteins similar to periplasmic molecular chaperone LolA, the outer membrane lipoprotein ...
 8-221 1.09e-49

proteins similar to periplasmic molecular chaperone LolA, the outer membrane lipoprotein receptor LolB and the periplasmic protein RseB; This family contains uncharacterized proteins similar to the periplasmic molecular chaperone LolA, the outer membrane lipoprotein receptor LolB and the periplasmic protein RseB, all of which have similar unclosed beta-barrel structures that resemble a baseball glove-like scaffold consisting of an 11-stranded antiparallel sheet. There are five Lol proteins (LolA, LolB, LolC, LolD, and LolE) involved in the sorting and membrane localization of lipoprotein and are highly conserved in Gram-negative bacteria. LolA accepts outer membrane (OM)-specific lipoproteins that are released from the inner membrane by the LolCDE complex and transfers them to the OM receptor LolB. It is proposed that the LolA/LolB complex forms a tunnel-like structure, where the hydrophobic insides of LolA and LolB are connected, which enables lipoproteins to transfer from LolA to LolB. RseB exerts a crucial role in modulating the stability of RseA, the transmembrane anti-sigma-factor that is degraded during sigma-E-dependent transcription caused by bacterial envelope stress. Its structural similarity to LolA and LolB suggests that RseA may act as a sensor of periplasmic stress with a dual functionality, detecting mislocalized lipoproteins as well as propagating the signal to induce the sigma-E-response.


Pssm-ID: 319986  Cd Length: 225  Bit Score: 161.71  E-value: 1.09e-49
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
3BUU_B        8 TAQSILEKADEIRFPQDSFQVNVAIRTAaPDHAEDLYRYQVLSKG--NENSIV*ITEPASERGQAIL-----*KGRDLWV 80
Cdd:cd16329   1 SAEEILEKVDDRRRGDSSADVSTMTLTD-KNGKERTRELRVYSKDggDDKSLIRFLSPADVKGTAFLtldydDKDDDQWL 79

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
3BUU_B       81 F*PSVSQPIRLSLSQR----LTGQVANGDIARANFTGDYHPQLLRNESIDDEDYYVLELTGID-RSVTYQKVLLWVNQSN 155
Cdd:cd16329  80 YLPALRRVRRISSSDKsqsfMGSDFSYEDLSRSRLLEDYTYKLLGEEEVDGRDCYVLELTPKDgADSGYSKRVLWVDKDT 159

                       170       180       190       200       210       220
                ....*....|....*....|....*....|....*....|....*....|....*....|....*..
3BUU_B      156 FRPYKAEFYSVSGRLLKTSRYENFDNILGE*RPTRII*EDaLKSGEVSVLDYSD*KLR-DLPDKIFT 221
Cdd:cd16329 160 FLPLKAEYYDRSGKLLKTLTFSDIKKIGGYWRPTRMEMKD-LLTGHKTVLEYSDIKFNvGLPDSLFT 225
LolA COG2834
Outer membrane lipoprotein-sorting protein [Cell wall/membrane/envelope biogenesis];
60-182 1.91e-06

Outer membrane lipoprotein-sorting protein [Cell wall/membrane/envelope biogenesis];


Pssm-ID: 442082  Cd Length: 211  Bit Score: 47.00  E-value: 1.91e-06
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
3BUU_B       60 ITEPAserGQAIL*KGRDLWVF*PSVSQPIRLSLSQRLTGQVANGDiaRANFTGDYHPQLLRNEsiDDEDYYVLELTGID 139
Cdd:COG2834  76 YTKPY---EQLIVSDGKTVWIYDPDLNQVTVIPLSDATPLALLLGD--FSDLLKDFTVTLLGEE--TGRKAYVLELTPKD 148

                        90       100       110       120
                ....*....|....*....|....*....|....*....|...
3BUU_B      140 RSVTYQKVLLWVNQSNFrPYKAEFYSVSGrllKTSRYEnFDNI 182
Cdd:COG2834 149 KDSGFGKITLWFDKETL-LRKLEIYDADG---QRTTIT-FSNV 186
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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