NCBI Home Page NCBI Site Search page NCBI Guide that lists and describes the NCBI resources
Conserved domains on  [gi|1159374967|pdb|5US1|J]
View 

Chain J, Aminoglycoside 2'-N-acetyltransferase

Protein Classification

GNAT family N-acetyltransferase( domain architecture ID 10006981)

GNAT family N-acetyltransferase catalyzes the transfer of an acetyl group from acetyl-CoA to a substrate

CATH:  3.40.630.30
EC:  2.3.-.-
Gene Ontology:  GO:0016746|GO:0008080
PubMed:  27367672|26818562|15581578|10940244
SCOP:  3000403

Graphical summary

 Zoom to residue level

show extra options »

Show site features     Horizontal zoom: ×

List of domain hits

 Name Accession Description Interval E-value
yhbS COG3153
Predicted N-acetyltransferase YhbS [General function prediction only];
23-140 7.76e-11

Predicted N-acetyltransferase YhbS [General function prediction only];


:

Pssm-ID: 442387 [Multi-domain]  Cd Length: 142  Bit Score: 57.40  E-value: 7.76e-11
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
5US1_J       23 QLTLSEKEALYDLLIEGF----EGDFSHDDFAHTLGGMHVMAFDQQKLVGHVAIIQRHMALDntpISVGYVEAMVVEQSY 98
Cdd:COG3153   3 PATPEDAEAIAALLRAAFgpgrEAELVDRLREDPAAGLSLVAEDDGEIVGHVALSPVDIDGE---GPALLLGPLAVDPEY 79

                        90       100       110       120
                ....*....|....*....|....*....|....*....|....*.
5US1_J       99 RRQGIGRQLMlqtNKIIASCYQLG----LLSASDDGQKLYHSVGWQ 140
Cdd:COG3153  80 RGQGIGRALM---RAALEAARERGaravVLLGDPSLLPFYERFGFR 122
 
Name Accession Description Interval E-value
yhbS COG3153
Predicted N-acetyltransferase YhbS [General function prediction only];
23-140 7.76e-11

Predicted N-acetyltransferase YhbS [General function prediction only];


Pssm-ID: 442387 [Multi-domain]  Cd Length: 142  Bit Score: 57.40  E-value: 7.76e-11
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
5US1_J       23 QLTLSEKEALYDLLIEGF----EGDFSHDDFAHTLGGMHVMAFDQQKLVGHVAIIQRHMALDntpISVGYVEAMVVEQSY 98
Cdd:COG3153   3 PATPEDAEAIAALLRAAFgpgrEAELVDRLREDPAAGLSLVAEDDGEIVGHVALSPVDIDGE---GPALLLGPLAVDPEY 79

                        90       100       110       120
                ....*....|....*....|....*....|....*....|....*.
5US1_J       99 RRQGIGRQLMlqtNKIIASCYQLG----LLSASDDGQKLYHSVGWQ 140
Cdd:COG3153  80 RGQGIGRALM---RAALEAARERGaravVLLGDPSLLPFYERFGFR 122
Acetyltransf_1 pfam00583
Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase ...
 30-123 2.55e-08

Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


Pssm-ID: 395465 [Multi-domain]  Cd Length: 116  Bit Score: 49.82  E-value: 2.55e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
5US1_J         30 EALYDLLIEGFEGDFSHD------DFAHTLGGMHVMAFDQQKLVGHVAIIQrhmalDNTPISVGYVEAMVVEQSYRRQGI 103
Cdd:pfam00583   2 EALYELLSEEFPEPWPDEpldlleDWDEDASEGFFVAEEDGELVGFASLSI-----IDDEPPVGEIEGLAVAPEYRGKGI 76

                          90       100
                  ....*....|....*....|
5US1_J        104 GRQLMlqtNKIIASCYQLGL 123
Cdd:pfam00583  77 GTALL---QALLEWARERGC 93
NAT_SF cd04301
N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer ...
 60-108 3.84e-05

N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer of an acyl group to a substrate; NAT (N-Acyltransferase) is a large superfamily of enzymes that mostly catalyze the transfer of an acyl group to a substrate and are implicated in a variety of functions, ranging from bacterial antibiotic resistance to circadian rhythms in mammals. Members include GCN5-related N-Acetyltransferases (GNAT) such as Aminoglycoside N-acetyltransferases, Histone N-acetyltransferase (HAT) enzymes, and Serotonin N-acetyltransferase, which catalyze the transfer of an acetyl group to a substrate. The kinetic mechanism of most GNATs involves the ordered formation of a ternary complex: the reaction begins with Acetyl Coenzyme A (AcCoA) binding, followed by binding of substrate, then direct transfer of the acetyl group from AcCoA to the substrate, followed by product and subsequent CoA release. Other family members include Arginine/ornithine N-succinyltransferase, Myristoyl-CoA: protein N-myristoyltransferase, and Acyl-homoserinelactone synthase which have a similar catalytic mechanism but differ in types of acyl groups transferred. Leucyl/phenylalanyl-tRNA-protein transferase and FemXAB nonribosomal peptidyltransferases which catalyze similar peptidyltransferase reactions are also included.


Pssm-ID: 173926 [Multi-domain]  Cd Length: 65  Bit Score: 39.95  E-value: 3.84e-05
                        10        20        30        40
                ....*....|....*....|....*....|....*....|....*....
5US1_J       60 AFDQQKLVGHVAIIQRHMALDntpisVGYVEAMVVEQSYRRQGIGRQLM 108
Cdd:cd04301   4 AEDDGEIVGFASLSPDGSGGD-----TAYIGDLAVLPEYRGKGIGSALL 47
PTZ00330 PTZ00330
acetyltransferase; Provisional
 85-140 3.60e-04

acetyltransferase; Provisional


Pssm-ID: 140351 [Multi-domain]  Cd Length: 147  Bit Score: 39.06  E-value: 3.60e-04
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*...
5US1_J        85 SVGYVEAMVVEQSYRRQGIGRQLMLQTNKIIAS--CYQLgLLSASDDGQKLYHSVGWQ 140
Cdd:PTZ00330  81 CVGHIEDVVVDPSYRGQGLGRALISDLCEIARSsgCYKV-ILDCTEDMVAFYKKLGFR 137
 
Name Accession Description Interval E-value
yhbS COG3153
Predicted N-acetyltransferase YhbS [General function prediction only];
23-140 7.76e-11

Predicted N-acetyltransferase YhbS [General function prediction only];


Pssm-ID: 442387 [Multi-domain]  Cd Length: 142  Bit Score: 57.40  E-value: 7.76e-11
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
5US1_J       23 QLTLSEKEALYDLLIEGF----EGDFSHDDFAHTLGGMHVMAFDQQKLVGHVAIIQRHMALDntpISVGYVEAMVVEQSY 98
Cdd:COG3153   3 PATPEDAEAIAALLRAAFgpgrEAELVDRLREDPAAGLSLVAEDDGEIVGHVALSPVDIDGE---GPALLLGPLAVDPEY 79

                        90       100       110       120
                ....*....|....*....|....*....|....*....|....*.
5US1_J       99 RRQGIGRQLMlqtNKIIASCYQLG----LLSASDDGQKLYHSVGWQ 140
Cdd:COG3153  80 RGQGIGRALM---RAALEAARERGaravVLLGDPSLLPFYERFGFR 122
Eis COG4552
Predicted acetyltransferase [General function prediction only];
23-108 1.41e-08

Predicted acetyltransferase [General function prediction only];


Pssm-ID: 443616 [Multi-domain]  Cd Length: 393  Bit Score: 53.36  E-value: 1.41e-08
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
5US1_J       23 QLTLSEKEALYDLLIEGFEGDFSHDDFAHTL----GGMHVMAFDQQKLVGHVAIIQRHMALDNTPISVGYVeAMV-VEQS 97
Cdd:COG4552   5 PLTEDDLDAFARLLAYAFGPEPDDEELEAYRpllePGRVLGVFDDGELVGTLALYPFTLNVGGARVPMAGI-TGVaVAPE 83

                        90
                ....*....|.
5US1_J       98 YRRQGIGRQLM 108
Cdd:COG4552  84 HRRRGVARALL 94
Acetyltransf_1 pfam00583
Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase ...
 30-123 2.55e-08

Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


Pssm-ID: 395465 [Multi-domain]  Cd Length: 116  Bit Score: 49.82  E-value: 2.55e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
5US1_J         30 EALYDLLIEGFEGDFSHD------DFAHTLGGMHVMAFDQQKLVGHVAIIQrhmalDNTPISVGYVEAMVVEQSYRRQGI 103
Cdd:pfam00583   2 EALYELLSEEFPEPWPDEpldlleDWDEDASEGFFVAEEDGELVGFASLSI-----IDDEPPVGEIEGLAVAPEYRGKGI 76

                          90       100
                  ....*....|....*....|
5US1_J        104 GRQLMlqtNKIIASCYQLGL 123
Cdd:pfam00583  77 GTALL---QALLEWARERGC 93
Acetyltransf_10 pfam13673
Acetyltransferase (GNAT) domain; This family contains proteins with N-acetyltransferase ...
 54-140 4.41e-08

Acetyltransferase (GNAT) domain; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


Pssm-ID: 463953 [Multi-domain]  Cd Length: 128  Bit Score: 49.58  E-value: 4.41e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
5US1_J         54 GGMHVM-AFDQQKLVGHVAIIQRhmaldntpisvGYVEAMVVEQSYRRQGIGRQLMlqtNKIIASCYQLGL------LSA 126
Cdd:pfam13673  29 GEYFFFvAFEGGQIVGVIALRDR-----------GHISLLFVDPDYQGQGIGKALL---EAVEDYAEKDGIklseltVNA 94

                          90
                  ....*....|....
5US1_J        127 SDDGQKLYHSVGWQ 140
Cdd:pfam13673  95 SPYAVPFYEKLGFR 108
ElaA COG2153
Predicted N-acyltransferase, GNAT family [General function prediction only];
57-141 1.79e-07

Predicted N-acyltransferase, GNAT family [General function prediction only];


Pssm-ID: 441756 [Multi-domain]  Cd Length: 134  Bit Score: 47.87  E-value: 1.79e-07
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
5US1_J       57 HVMAFDQQKLVGHVAIIQrhmaldnTPISVGYVEAMVVEQSYRRQGIGRQLMlqtNKIIASCYQLGL----LSASDDGQK 132
Cdd:COG2153  36 HLLAYDDGELVATARLLP-------PGDGEAKIGRVAVLPEYRGQGLGRALM---EAAIEEARERGArrivLSAQAHAVG 105


                ....*....
5US1_J      133 LYHSVGWQI 141
Cdd:COG2153 106 FYEKLGFVP 114
Acetyltransf_9 pfam13527
Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.
 24-139 3.10e-07

Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.


Pssm-ID: 404421 [Multi-domain]  Cd Length: 124  Bit Score: 47.18  E-value: 3.10e-07
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
5US1_J         24 LTLSEKEALYDLLIEGFEGDFSHDD---FAHTLG-GMHVMAFDQQKLVGHVAIIQRHMALDNTPISVGYVEAMVVEQSYR 99
Cdd:pfam13527   4 LTEDEFDEVLRLLEYAFQDEDSPELreyFRPLLEeGRVLGAFDDGELVSTLALYPFELNVPGKTLPAAGITGVATYPEYR 83

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....
5US1_J        100 RQGIGRQLMlqtNKIIASCYQ----LGLLSASDDGqkLYHSVGW 139
Cdd:pfam13527  84 GRGVMSRLL---RRSLEEMRErgvpLSFLYPSSYP--IYRRFGY 122
Acetyltransf_7 pfam13508
Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.
 60-141 1.39e-06

Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.


Pssm-ID: 463905 [Multi-domain]  Cd Length: 84  Bit Score: 44.37  E-value: 1.39e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
5US1_J         60 AFDQQKLVGHVAIIQRHmaldntPISVGYVEAMVVEQSYRRQGIGRQLMLQTNKII-ASCYQLGLLSASDDGQKLYHSVG 138
Cdd:pfam13508   8 AEDDGKIVGFAALLPLD------DEGALAELRLAVHPEYRGQGIGRALLEAAEAAAkEGGIKLLELETTNRAAAFYEKLG 81


                  ...
5US1_J        139 WQI 141
Cdd:pfam13508  82 FEE 84
ArgA COG1246
N-acetylglutamate synthase or related acetyltransferase, GNAT family [Amino acid transport and ...
 25-140 7.81e-06

N-acetylglutamate synthase or related acetyltransferase, GNAT family [Amino acid transport and metabolism]; N-acetylglutamate synthase or related acetyltransferase, GNAT family is part of the Pathway/BioSystem: Arginine biosynthesis


Pssm-ID: 440859 [Multi-domain]  Cd Length: 132  Bit Score: 43.44  E-value: 7.81e-06
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
5US1_J       25 TLSEKEALYDLlIEGFEGDFSHDDFahtlggmhVMAFDQQKLVGHVAIIQRhmaldntPISVGYVEAMVVEQSYRRQGIG 104
Cdd:COG1246   7 TPDDVPAILEL-IRPYALEEEIGEF--------WVAEEDGEIVGCAALHPL-------DEDLAELRSLAVHPDYRGRGIG 70

                        90       100       110       120
                ....*....|....*....|....*....|....*....|
5US1_J      105 RQLMlqtNKIIASCYQLGL----LSASDDGQKLYHSVGWQ 140
Cdd:COG1246  71 RRLL---EALLAEARELGLkrlfLLTTSAAIHFYEKLGFE 107
PhnO COG0454
N-acetyltransferase, GNAT superfamily (includes histone acetyltransferase HPA2) [Transcription, ...
 43-141 2.12e-05

N-acetyltransferase, GNAT superfamily (includes histone acetyltransferase HPA2) [Transcription, General function prediction only];


Pssm-ID: 440222 [Multi-domain]  Cd Length: 136  Bit Score: 42.35  E-value: 2.12e-05
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
5US1_J       43 DFSHDDFAHTLGGMHVMAF----DQQKLVGHVAIiqRHMALDntpisVGYVEAMVVEQSYRRQGIGRQLM-----LQTNK 113
Cdd:COG0454  18 EALDAELKAMEGSLAGAEFiavdDKGEPIGFAGL--RRLDDK-----VLELKRLYVLPEYRGKGIGKALLealleWARER 90

                        90       100
                ....*....|....*....|....*...
5US1_J      114 IIASCYqLGLLSASDDGQKLYHSVGWQI 141
Cdd:COG0454  91 GCTALE-LDTLDGNPAAIRFYERLGFKE 117
NAT_SF cd04301
N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer ...
 60-108 3.84e-05

N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer of an acyl group to a substrate; NAT (N-Acyltransferase) is a large superfamily of enzymes that mostly catalyze the transfer of an acyl group to a substrate and are implicated in a variety of functions, ranging from bacterial antibiotic resistance to circadian rhythms in mammals. Members include GCN5-related N-Acetyltransferases (GNAT) such as Aminoglycoside N-acetyltransferases, Histone N-acetyltransferase (HAT) enzymes, and Serotonin N-acetyltransferase, which catalyze the transfer of an acetyl group to a substrate. The kinetic mechanism of most GNATs involves the ordered formation of a ternary complex: the reaction begins with Acetyl Coenzyme A (AcCoA) binding, followed by binding of substrate, then direct transfer of the acetyl group from AcCoA to the substrate, followed by product and subsequent CoA release. Other family members include Arginine/ornithine N-succinyltransferase, Myristoyl-CoA: protein N-myristoyltransferase, and Acyl-homoserinelactone synthase which have a similar catalytic mechanism but differ in types of acyl groups transferred. Leucyl/phenylalanyl-tRNA-protein transferase and FemXAB nonribosomal peptidyltransferases which catalyze similar peptidyltransferase reactions are also included.


Pssm-ID: 173926 [Multi-domain]  Cd Length: 65  Bit Score: 39.95  E-value: 3.84e-05
                        10        20        30        40
                ....*....|....*....|....*....|....*....|....*....
5US1_J       60 AFDQQKLVGHVAIIQRHMALDntpisVGYVEAMVVEQSYRRQGIGRQLM 108
Cdd:cd04301   4 AEDDGEIVGFASLSPDGSGGD-----TAYIGDLAVLPEYRGKGIGSALL 47
RimI COG0456
Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal ...
 86-141 6.32e-05

Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal structure and biogenesis];


Pssm-ID: 440224 [Multi-domain]  Cd Length: 92  Bit Score: 40.02  E-value: 6.32e-05
                        10        20        30        40        50        60
                ....*....|....*....|....*....|....*....|....*....|....*....|...
5US1_J       86 VGYVEAMVVEQSYRRQGIGRQLMlqtNKIIASCYQLGL-------LSASDDGQKLYHSVGWQI 141
Cdd:COG0456  13 EAEIEDLAVDPEYRGRGIGRALL---EAALERARERGArrlrlevREDNEAAIALYEKLGFEE 72
PTZ00330 PTZ00330
acetyltransferase; Provisional
 85-140 3.60e-04

acetyltransferase; Provisional


Pssm-ID: 140351 [Multi-domain]  Cd Length: 147  Bit Score: 39.06  E-value: 3.60e-04
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*...
5US1_J        85 SVGYVEAMVVEQSYRRQGIGRQLMLQTNKIIAS--CYQLgLLSASDDGQKLYHSVGWQ 140
Cdd:PTZ00330  81 CVGHIEDVVVDPSYRGQGLGRALISDLCEIARSsgCYKV-ILDCTEDMVAFYKKLGFR 137
COG3393 COG3393
Predicted acetyltransferase, GNAT family [General function prediction only];
85-140 1.21e-03

Predicted acetyltransferase, GNAT family [General function prediction only];


Pssm-ID: 442620 [Multi-domain]  Cd Length: 86  Bit Score: 36.43  E-value: 1.21e-03
                        10        20        30        40        50        60
                ....*....|....*....|....*....|....*....|....*....|....*....|...
5US1_J       85 SVGYVEAMVVEQSYRRQGIGRQLM-------LQTNKIIAScyqLGLLSASDDGQKLYHSVGWQ 140
Cdd:COG3393  14 GVAEISGVYTHPEYRGRGLASALVaalareaLARGARTPF---LYVDADNPAARRLYERLGFR 73
PRK10314 PRK10314
GNAT family N-acetyltransferase;
14-139 2.38e-03

GNAT family N-acetyltransferase;


Pssm-ID: 182373 [Multi-domain]  Cd Length: 153  Bit Score: 36.75  E-value: 2.38e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
5US1_J        14 IEYRSLHTSQLTLSEKEALYDLLIEGF-------EGDFSHDDFahTLGGMHVMAFDQQKLVGHVAIIQRHMALDntPISV 86
Cdd:PRK10314   2 IEWQDLHHSELSVSQLYALLQLRCAVFvveqncpYQDIDGDDL--TGDNRHILGWKNDELVAYARILKSDDDLE--PVVI 77

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....*...
5US1_J        87 GYVeamVVEQSYRRQGIGRQLMlqtNKIIASCYQLG-----LLSASDDGQKLYHSVGW 139
Cdd:PRK10314  78 GRV---IVSEALRGEKVGQQLM---SKTLESCTRHWpdkpvYLGAQAHLQNFYQSFGF 129
MnaT COG1247
L-amino acid N-acyltransferase MnaT [Amino acid transport and metabolism];
88-142 4.38e-03

L-amino acid N-acyltransferase MnaT [Amino acid transport and metabolism];


Pssm-ID: 440860 [Multi-domain]  Cd Length: 163  Bit Score: 36.13  E-value: 4.38e-03
                        10        20        30        40        50        60
                ....*....|....*....|....*....|....*....|....*....|....*....|..
5US1_J       88 YVEAMVVEQSYRRQGIGRQLMlqtNKIIASCYQLGL-------LSASDDGQKLYHSVGWQIW 142
Cdd:COG1247  82 AEESIYVDPDARGRGIGRALL---EALIERARARGYrrlvavvLADNEASIALYEKLGFEEV 140
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
Help | Disclaimer | Write to the Help Desk
NCBI | NLM | NIH