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Conserved domains on  [gi|2623050|gb|AAB86376|]
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biotin acetyl-CoA carboxylase ligase/biotin operon repressor [Methanothermobacter thermautotrophicus str. Delta H]

Protein Classification

lipoate--protein ligase family protein; lipoate--protein ligase( domain architecture ID 11488633)

lipoate--protein ligase family protein, similar to Staphylococcus aureus lipoate--protein ligase 1 and Saccharomyces cerevisiae octanoyltransferase| lipoate--protein ligase catalyzes both the ATP-dependent activation of exogenously supplied lipoate to lipoyl-AMP and the transfer of the activated lipoyl onto the lipoyl domains of lipoate-dependent enzymes; can also use octanoate as substrate

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
birA_ligase TIGR00121
birA, biotin-[acetyl-CoA-carboxylase] ligase region; This model represents the ...
 20-261 4.69e-115

birA, biotin-[acetyl-CoA-carboxylase] ligase region; This model represents the biotin--acetyl-CoA-carboxylase ligase region of biotin--acetyl-CoA-carboxylase ligase. In Escherichia coli and some other species, this enzyme is part of a bifunction protein BirA that includes a small, N-terminal biotin operon repressor domain. Proteins identified by this model should not be called bifunctional unless they are also identified by birA_repr_reg (TIGR00122). The protein name suggests that this enzyme transfers biotin only to acetyl-CoA-carboxylase but it also transfers the biotin moiety to other proteins. The apparent orthologs among the eukaryotes are larger proteins that contain a single copy of this domain. [Protein fate, Protein modification and repair]


:

Pssm-ID: 272917 [Multi-domain]  Cd Length: 237  Bit Score: 329.75  E-value: 4.69e-115
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2623050     20 EIKCFDEVDSTNNVAKRMAEDGATEGTVVIAKTQSRGRGRRGKPWISPQGGIWMSIILRPEVHPSRAPLLTLVAGVAVAR 99
Cdd:TIGR00121   1 EVIVLDVIDSTNQYALELAKEGKLKGDLVVAEYQTAGRGRRGRKWLSPEGGLYFSLILRPDLPKSPAPGLTLVAGIAIAE 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2623050    100 TLRDEcGLDVGIKWPNDILIGDKKVCGILTEAHARFNTLEYVVVGVGIdtNVDISHFPDDLREGATSIKNELKRDIKSSE 179
Cdd:TIGR00121  81 VLKEL-GDQVQVKWPNDILLKDKKLGGILTELTGKENRADYVVIGIGI--NVQNRKPAESLREQAISLSEEAGIDLDRGE 157

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2623050    180 LIARFLRNFEETYNSFKDGKMDEILTEWRKLSKTIGRRVEIRKQLGEIVhGDAVGINSEGALILElDDGTLRKVISGECI 259
Cdd:TIGR00121 158 LIEGFLRNFEENLEWFEQEGIDEILSKWEKLSAHIGREVSLTTGNGEIE-GIARGIDKDGALLLE-DGGGIKKIISGEIS 235


                  ..
gi 2623050    260 HR 261
Cdd:TIGR00121 236 LR 237
 
Name Accession Description Interval E-value
birA_ligase TIGR00121
birA, biotin-[acetyl-CoA-carboxylase] ligase region; This model represents the ...
 20-261 4.69e-115

birA, biotin-[acetyl-CoA-carboxylase] ligase region; This model represents the biotin--acetyl-CoA-carboxylase ligase region of biotin--acetyl-CoA-carboxylase ligase. In Escherichia coli and some other species, this enzyme is part of a bifunction protein BirA that includes a small, N-terminal biotin operon repressor domain. Proteins identified by this model should not be called bifunctional unless they are also identified by birA_repr_reg (TIGR00122). The protein name suggests that this enzyme transfers biotin only to acetyl-CoA-carboxylase but it also transfers the biotin moiety to other proteins. The apparent orthologs among the eukaryotes are larger proteins that contain a single copy of this domain. [Protein fate, Protein modification and repair]


Pssm-ID: 272917 [Multi-domain]  Cd Length: 237  Bit Score: 329.75  E-value: 4.69e-115
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2623050     20 EIKCFDEVDSTNNVAKRMAEDGATEGTVVIAKTQSRGRGRRGKPWISPQGGIWMSIILRPEVHPSRAPLLTLVAGVAVAR 99
Cdd:TIGR00121   1 EVIVLDVIDSTNQYALELAKEGKLKGDLVVAEYQTAGRGRRGRKWLSPEGGLYFSLILRPDLPKSPAPGLTLVAGIAIAE 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2623050    100 TLRDEcGLDVGIKWPNDILIGDKKVCGILTEAHARFNTLEYVVVGVGIdtNVDISHFPDDLREGATSIKNELKRDIKSSE 179
Cdd:TIGR00121  81 VLKEL-GDQVQVKWPNDILLKDKKLGGILTELTGKENRADYVVIGIGI--NVQNRKPAESLREQAISLSEEAGIDLDRGE 157

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2623050    180 LIARFLRNFEETYNSFKDGKMDEILTEWRKLSKTIGRRVEIRKQLGEIVhGDAVGINSEGALILElDDGTLRKVISGECI 259
Cdd:TIGR00121 158 LIEGFLRNFEENLEWFEQEGIDEILSKWEKLSAHIGREVSLTTGNGEIE-GIARGIDKDGALLLE-DGGGIKKIISGEIS 235


                  ..
gi 2623050    260 HR 261
Cdd:TIGR00121 236 LR 237
BirA2 COG0340
Biotin-(acetyl-CoA carboxylase) ligase [Coenzyme transport and metabolism]; Biotin-(acetyl-CoA ...
 20-261 1.37e-107

Biotin-(acetyl-CoA carboxylase) ligase [Coenzyme transport and metabolism]; Biotin-(acetyl-CoA carboxylase) ligase is part of the Pathway/BioSystem: Biotin biosynthesis


Pssm-ID: 440109 [Multi-domain]  Cd Length: 241  Bit Score: 310.95  E-value: 1.37e-107
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2623050   20 EIKCFDEVDSTNNVAKRMAEDGATEGTVVIAKTQSRGRGRRGKPWISPQG-GIWMSIILRPEVHPSRAPLLTLVAGVAVA 98
Cdd:COG0340   1 RIEVFDEVDSTNDEAKELAREGAPEGTVVVAEEQTAGRGRRGRSWVSPPGkGLYFSLLLRPDLPPARLPLLSLAAGLAVA 80

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2623050   99 RTLRDECGLDVGIKWPNDILIGDKKVCGILTEAHARFNTLEYVVVGVGIdtNVDISHF-PDDLREGATSIKNELKRDIKS 177
Cdd:COG0340  81 EALRELTGVDVGLKWPNDILLNGKKLAGILIEASGEGDGIDWVVIGIGI--NVNQPPFdPEELDQPATSLKEETGKEVDR 158

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2623050  178 SELIARFLRNFEETYNSFKDGKMDEILTEWRKLSKTIGRRVEIRKQlGEIVHGDAVGINSEGALILELDDGTLRKVISGE 257
Cdd:COG0340 159 EELLAALLEELEELYDRFLEEGFAPILEEWRARLATLGRRVRVETG-GETLEGIAVGIDEDGALLLETADGEIRAVAAGE 237


                ....
gi 2623050  258 CIHR 261
Cdd:COG0340 238 VSLR 241
BPL cd16442
biotin protein ligase; Biotin protein ligase (EC 6.3.4.15) catalyzes the synthesis of an ...
 21-190 8.01e-77

biotin protein ligase; Biotin protein ligase (EC 6.3.4.15) catalyzes the synthesis of an activated form of biotin, biotinyl-5'-AMP, from substrates biotin and ATP followed by biotinylation of the biotin carboxyl carrier protein subunit of acetyl-CoA carboxylase. Biotin protein ligase (BPL) is the enzyme responsible for attaching biotin to a specific lysine at the active site of biotin enzymes. Biotin attachment is a two step reaction that results in the formation of an amide linkage between the carboxyl group of biotin and the epsilon-amino group of the modified lysine.


Pssm-ID: 319741 [Multi-domain]  Cd Length: 173  Bit Score: 230.23  E-value: 8.01e-77
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2623050   21 IKCFDEVDSTNNVAKRMAEDGATEGTVVIAKTQSRGRGRRGKPWISPQG-GIWMSIILRPEVHPSRAPLLTLVAGVAVAR 99
Cdd:cd16442   2 LIVLDEIDSTNDEAKELARSGAPEGTVVVAEEQTAGRGRRGRKWESPKGkGLYFSLLLRPDVPPAEAPLLTLLAAVAVAE 81

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2623050  100 TLRDECGLDVGIKWPNDILIGDKKVCGILTEAHARFNTLEYVVVGVGIDTNVDISHFPDDLREGATSIKNELKRDIKSSE 179
Cdd:cd16442  82 ALEKLGGIPVQIKWPNDILVNGKKLAGILTEASAEGEGVAAVVIGIGINVNNTPPPEPLPDTSLATSLGKEVDRNELLEE 161

                       170
                ....*....|.
gi 2623050  180 LIARFLRNFEE 190
Cdd:cd16442 162 LLAALENRLEL 172
PRK08330 PRK08330
biotin--protein ligase; Provisional
 17-257 2.50e-69

biotin--protein ligase; Provisional


Pssm-ID: 169384 [Multi-domain]  Cd Length: 236  Bit Score: 213.45  E-value: 2.50e-69
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2623050    17 IGHEIKCFDEVDSTNNVAKRMAEDgATEGTVVIAKTQSRGRGRRGKPWISPQGGIWMSIILRPEVHPSRAPLLTLVAGVA 96
Cdd:PRK08330   1 IGRNIIYFDEVDSTNEYAKRIAPD-EEEGTVIVADRQTAGHGRKGRAWASPEGGLWMSVILKPKVSPEHLPKLVFLGALA 79

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2623050    97 VARTLRdECGLDVGIKWPNDILIGDKKVCGILTEAHArfntlEYVVVGVGIDTNVDIshfPDDLREGATSIKNELKRDIK 176
Cdd:PRK08330  80 VVDTLR-EFGIEGKIKWPNDVLVNYKKIAGVLVEGKG-----DFVVLGIGLNVNNEI---PDELRETATSMKEVLGREVP 150

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2623050   177 SSELIARFLRNFEETYNSFKDGKMdEILTEWRKLSKTIGRRVEIRKQLGEIVHGDAVGINSEGALILELDDGTLRKVISG 256
Cdd:PRK08330 151 LIEVFKRLVENLDRWYKLFLEGPG-EILEEVKGRSMILGKRVKIIGDGEILVEGIAEDIDEFGALILRLDDGTVKKVLYG 229


                 .
gi 2623050   257 E 257
Cdd:PRK08330 230 D 230
BPL_LplA_LipB pfam03099
Biotin/lipoate A/B protein ligase family; This family includes biotin protein ligase, ...
 27-148 3.62e-22

Biotin/lipoate A/B protein ligase family; This family includes biotin protein ligase, lipoate-protein ligase A and B. Biotin is covalently attached at the active site of certain enzymes that transfer carbon dioxide from bicarbonate to organic acids to form cellular metabolites. Biotin protein ligase (BPL) is the enzyme responsible for attaching biotin to a specific lysine at the active site of biotin enzymes. Each organizm probably has only one BPL. Biotin attachment is a two step reaction that results in the formation of an amide linkage between the carboxyl group of biotin and the epsilon-amino group of the modified lysine. Lipoate-protein ligase A (LPLA) catalyzes the formation of an amide linkage between lipoic acid and a specific lysine residue in lipoate dependent enzymes. The unusual biosynthesis pathway of lipoic acid is mechanistically intertwined with attachment of the cofactor.


Pssm-ID: 427135  Cd Length: 132  Bit Score: 88.65  E-value: 3.62e-22
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2623050     27 VDSTNNVAKRMAEDGATEGTVVIAKTQSRGRGRRGKPWISPQGGIWMSIILRPE---VHPSRAPLLTLVAGVAVARTLRD 103
Cdd:pfam03099   5 IKSTNTYLEELNSSELESGGVVVVRRQTGGRGRGGNVWHSPKGCLTYSLLLSKEhpnVDPSVLEFYVLELVLAVLEALGL 84

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*....
gi 2623050    104 ----ECGLDVGIKWPNDILIGDKKVCGILTEAHARfNTLEYVVVGVGID 148
Cdd:pfam03099  85 ykpgISGIPCFVKWPNDLYVNGRKLAGILQRSTRG-GTLHHGVIGLGVN 132
 
Name Accession Description Interval E-value
birA_ligase TIGR00121
birA, biotin-[acetyl-CoA-carboxylase] ligase region; This model represents the ...
 20-261 4.69e-115

birA, biotin-[acetyl-CoA-carboxylase] ligase region; This model represents the biotin--acetyl-CoA-carboxylase ligase region of biotin--acetyl-CoA-carboxylase ligase. In Escherichia coli and some other species, this enzyme is part of a bifunction protein BirA that includes a small, N-terminal biotin operon repressor domain. Proteins identified by this model should not be called bifunctional unless they are also identified by birA_repr_reg (TIGR00122). The protein name suggests that this enzyme transfers biotin only to acetyl-CoA-carboxylase but it also transfers the biotin moiety to other proteins. The apparent orthologs among the eukaryotes are larger proteins that contain a single copy of this domain. [Protein fate, Protein modification and repair]


Pssm-ID: 272917 [Multi-domain]  Cd Length: 237  Bit Score: 329.75  E-value: 4.69e-115
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2623050     20 EIKCFDEVDSTNNVAKRMAEDGATEGTVVIAKTQSRGRGRRGKPWISPQGGIWMSIILRPEVHPSRAPLLTLVAGVAVAR 99
Cdd:TIGR00121   1 EVIVLDVIDSTNQYALELAKEGKLKGDLVVAEYQTAGRGRRGRKWLSPEGGLYFSLILRPDLPKSPAPGLTLVAGIAIAE 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2623050    100 TLRDEcGLDVGIKWPNDILIGDKKVCGILTEAHARFNTLEYVVVGVGIdtNVDISHFPDDLREGATSIKNELKRDIKSSE 179
Cdd:TIGR00121  81 VLKEL-GDQVQVKWPNDILLKDKKLGGILTELTGKENRADYVVIGIGI--NVQNRKPAESLREQAISLSEEAGIDLDRGE 157

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2623050    180 LIARFLRNFEETYNSFKDGKMDEILTEWRKLSKTIGRRVEIRKQLGEIVhGDAVGINSEGALILElDDGTLRKVISGECI 259
Cdd:TIGR00121 158 LIEGFLRNFEENLEWFEQEGIDEILSKWEKLSAHIGREVSLTTGNGEIE-GIARGIDKDGALLLE-DGGGIKKIISGEIS 235


                  ..
gi 2623050    260 HR 261
Cdd:TIGR00121 236 LR 237
BirA2 COG0340
Biotin-(acetyl-CoA carboxylase) ligase [Coenzyme transport and metabolism]; Biotin-(acetyl-CoA ...
 20-261 1.37e-107

Biotin-(acetyl-CoA carboxylase) ligase [Coenzyme transport and metabolism]; Biotin-(acetyl-CoA carboxylase) ligase is part of the Pathway/BioSystem: Biotin biosynthesis


Pssm-ID: 440109 [Multi-domain]  Cd Length: 241  Bit Score: 310.95  E-value: 1.37e-107
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2623050   20 EIKCFDEVDSTNNVAKRMAEDGATEGTVVIAKTQSRGRGRRGKPWISPQG-GIWMSIILRPEVHPSRAPLLTLVAGVAVA 98
Cdd:COG0340   1 RIEVFDEVDSTNDEAKELAREGAPEGTVVVAEEQTAGRGRRGRSWVSPPGkGLYFSLLLRPDLPPARLPLLSLAAGLAVA 80

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2623050   99 RTLRDECGLDVGIKWPNDILIGDKKVCGILTEAHARFNTLEYVVVGVGIdtNVDISHF-PDDLREGATSIKNELKRDIKS 177
Cdd:COG0340  81 EALRELTGVDVGLKWPNDILLNGKKLAGILIEASGEGDGIDWVVIGIGI--NVNQPPFdPEELDQPATSLKEETGKEVDR 158

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2623050  178 SELIARFLRNFEETYNSFKDGKMDEILTEWRKLSKTIGRRVEIRKQlGEIVHGDAVGINSEGALILELDDGTLRKVISGE 257
Cdd:COG0340 159 EELLAALLEELEELYDRFLEEGFAPILEEWRARLATLGRRVRVETG-GETLEGIAVGIDEDGALLLETADGEIRAVAAGE 237


                ....
gi 2623050  258 CIHR 261
Cdd:COG0340 238 VSLR 241
BPL cd16442
biotin protein ligase; Biotin protein ligase (EC 6.3.4.15) catalyzes the synthesis of an ...
 21-190 8.01e-77

biotin protein ligase; Biotin protein ligase (EC 6.3.4.15) catalyzes the synthesis of an activated form of biotin, biotinyl-5'-AMP, from substrates biotin and ATP followed by biotinylation of the biotin carboxyl carrier protein subunit of acetyl-CoA carboxylase. Biotin protein ligase (BPL) is the enzyme responsible for attaching biotin to a specific lysine at the active site of biotin enzymes. Biotin attachment is a two step reaction that results in the formation of an amide linkage between the carboxyl group of biotin and the epsilon-amino group of the modified lysine.


Pssm-ID: 319741 [Multi-domain]  Cd Length: 173  Bit Score: 230.23  E-value: 8.01e-77
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2623050   21 IKCFDEVDSTNNVAKRMAEDGATEGTVVIAKTQSRGRGRRGKPWISPQG-GIWMSIILRPEVHPSRAPLLTLVAGVAVAR 99
Cdd:cd16442   2 LIVLDEIDSTNDEAKELARSGAPEGTVVVAEEQTAGRGRRGRKWESPKGkGLYFSLLLRPDVPPAEAPLLTLLAAVAVAE 81

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2623050  100 TLRDECGLDVGIKWPNDILIGDKKVCGILTEAHARFNTLEYVVVGVGIDTNVDISHFPDDLREGATSIKNELKRDIKSSE 179
Cdd:cd16442  82 ALEKLGGIPVQIKWPNDILVNGKKLAGILTEASAEGEGVAAVVIGIGINVNNTPPPEPLPDTSLATSLGKEVDRNELLEE 161

                       170
                ....*....|.
gi 2623050  180 LIARFLRNFEE 190
Cdd:cd16442 162 LLAALENRLEL 172
PRK08330 PRK08330
biotin--protein ligase; Provisional
 17-257 2.50e-69

biotin--protein ligase; Provisional


Pssm-ID: 169384 [Multi-domain]  Cd Length: 236  Bit Score: 213.45  E-value: 2.50e-69
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2623050    17 IGHEIKCFDEVDSTNNVAKRMAEDgATEGTVVIAKTQSRGRGRRGKPWISPQGGIWMSIILRPEVHPSRAPLLTLVAGVA 96
Cdd:PRK08330   1 IGRNIIYFDEVDSTNEYAKRIAPD-EEEGTVIVADRQTAGHGRKGRAWASPEGGLWMSVILKPKVSPEHLPKLVFLGALA 79

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2623050    97 VARTLRdECGLDVGIKWPNDILIGDKKVCGILTEAHArfntlEYVVVGVGIDTNVDIshfPDDLREGATSIKNELKRDIK 176
Cdd:PRK08330  80 VVDTLR-EFGIEGKIKWPNDVLVNYKKIAGVLVEGKG-----DFVVLGIGLNVNNEI---PDELRETATSMKEVLGREVP 150

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2623050   177 SSELIARFLRNFEETYNSFKDGKMdEILTEWRKLSKTIGRRVEIRKQLGEIVHGDAVGINSEGALILELDDGTLRKVISG 256
Cdd:PRK08330 151 LIEVFKRLVENLDRWYKLFLEGPG-EILEEVKGRSMILGKRVKIIGDGEILVEGIAEDIDEFGALILRLDDGTVKKVLYG 229


                 .
gi 2623050   257 E 257
Cdd:PRK08330 230 D 230
PRK11886 PRK11886
bifunctional biotin--[acetyl-CoA-carboxylase] ligase/biotin operon repressor BirA;
24-257 1.08e-56

bifunctional biotin--[acetyl-CoA-carboxylase] ligase/biotin operon repressor BirA;


Pssm-ID: 237010 [Multi-domain]  Cd Length: 319  Bit Score: 183.84  E-value: 1.08e-56
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2623050    24 FDEVDSTNNVAK-RMAEDGAteGTVVIAKTQSRGRGRRGKPWISPQGG-IWMSIILRPEVHPSRAPLLTLVAGVAVARTL 101
Cdd:PRK11886  83 LPVIDSTNQYLLdRIAELKS--GDLCLAEYQTAGRGRRGRQWFSPFGGnLYLSLYWRLNQGPAQAMGLSLVVGIAIAEAL 160

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2623050   102 RDECGLDVGIKWPNDILIGDKKVCGILTEAHARFNTLEYVVVGVGIdtNVDISHFPDDLREGATSIKNELKRDIKSSELI 181
Cdd:PRK11886 161 RRLGAIDVGLKWPNDIYLNDRKLAGILVELSGETGDAAHVVIGIGI--NVAMPDFPEELIDQPWSDLQEAGPTIDRNQLA 238

                        170       180       190       200       210       220       230
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 2623050   182 ARFLRNFEETYNSFKDGKMDEILTEWRKLSKTIGRRVEIrkQLGE-IVHGDAVGINSEGALILElDDGTLRKVISGE 257
Cdd:PRK11886 239 AELIKQLRAALELFEQEGLAPFLERWKKLDLFLGREVKL--IIGDkEISGIARGIDEQGALLLE-DDGVEKPFNGGE 312
BirA COG1654
Biotin operon repressor [Transcription];
13-257 6.89e-43

Biotin operon repressor [Transcription];


Pssm-ID: 441260 [Multi-domain]  Cd Length: 324  Bit Score: 148.21  E-value: 6.89e-43
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2623050   13 DTEYIGHEIKCFDEVDSTNNVAKRMAEDGATEGTVVIAKTQSRGRGRRGKPWISPQGG-IWMSIILRPEVHPSRAPLLTL 91
Cdd:COG1654  76 STKRLGREILYVISSTSTNLLALELAAQGGDAGTVVAAEQQRGGRGRRRRSWSSPGGGgLLYSLLLRPPIAPALLSLLLL 155

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2623050   92 VAGVAVARTLRDECGLDVGIKWPNDILIGDKKVCGILTEAHARFNTLEYVVVGVGIDTNVDISHFPDDLREGATSIKNEL 171
Cdd:COG1654 156 AAAVAVAAALAEGGGLVKWKKWPNDLLKKGKKILGILEEEGGDADGVVIVVGGGGNNNNSNPEEEPQELAELATSLLLIL 235

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2623050  172 KRDIKSSELIARFLRNFEETYNSFKDGKMDEILTEWRKLSKTIGRRVEIRKQLGEIVhGDAVGINSEGALILELDDGTLR 251
Cdd:COG1654 236 RLRLLRLLLLLLLLLLELLELLGFLEFFFLWERLDWELLRVLKLVVVVVEIGGGGGG-GGALGGGLLGLLLLGGGGGGGE 314


                ....*.
gi 2623050  252 KVISGE 257
Cdd:COG1654 315 GSLSAV 320
PTZ00276 PTZ00276
biotin/lipoate protein ligase; Provisional
 21-250 7.35e-23

biotin/lipoate protein ligase; Provisional


Pssm-ID: 140302 [Multi-domain]  Cd Length: 245  Bit Score: 93.78  E-value: 7.35e-23
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2623050    21 IKCFDEVDSTNNVAKRMAEDGATEGTVVIAKTQSRGRGRRGKPWISPQGGIWMSIILrPE--VHPSRAPLLTLVAGVAVA 98
Cdd:PTZ00276   9 IHFVGEVTSTMDVARTMLAAAGGKPFAVLAESQTAGRGTGGRTWTSPKGNMYFTLCI-PQkgVPPELVPVLPLITGLACR 87

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2623050    99 RTLRDEC-GLDVGIKWPNDILIGDKKVCGILTEAHArfntlEYVVVGVGIdtNVDIS-HFPDDLREgATSIkNELKRDIK 176
Cdd:PTZ00276  88 AAIMEVLhGAAVHTKWPNDIIYAGKKIGGSLIESEG-----EYLIIGIGM--NIEVApPVTDAGRE-STMV-NEIAEDLG 158

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2623050   177 SSELIARFL-----RNF-----------EETYNSFkDGKMDEILTEWRKLSKtiGRRVEirkqlgEIVhgdAVGINSEGA 240
Cdd:PTZ00276 159 VKSVTPQDLaeavwKHFfdicsdpeltrEILIESF-DAAMDKSLKLHKRTPT--GRDPE------ELT---ALSLNEWGH 226

                        250
                 ....*....|
gi 2623050   241 LILELDDGTL 250
Cdd:PTZ00276 227 LIVRRPDGTE 236
BPL_LplA_LipB pfam03099
Biotin/lipoate A/B protein ligase family; This family includes biotin protein ligase, ...
 27-148 3.62e-22

Biotin/lipoate A/B protein ligase family; This family includes biotin protein ligase, lipoate-protein ligase A and B. Biotin is covalently attached at the active site of certain enzymes that transfer carbon dioxide from bicarbonate to organic acids to form cellular metabolites. Biotin protein ligase (BPL) is the enzyme responsible for attaching biotin to a specific lysine at the active site of biotin enzymes. Each organizm probably has only one BPL. Biotin attachment is a two step reaction that results in the formation of an amide linkage between the carboxyl group of biotin and the epsilon-amino group of the modified lysine. Lipoate-protein ligase A (LPLA) catalyzes the formation of an amide linkage between lipoic acid and a specific lysine residue in lipoate dependent enzymes. The unusual biosynthesis pathway of lipoic acid is mechanistically intertwined with attachment of the cofactor.


Pssm-ID: 427135  Cd Length: 132  Bit Score: 88.65  E-value: 3.62e-22
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2623050     27 VDSTNNVAKRMAEDGATEGTVVIAKTQSRGRGRRGKPWISPQGGIWMSIILRPE---VHPSRAPLLTLVAGVAVARTLRD 103
Cdd:pfam03099   5 IKSTNTYLEELNSSELESGGVVVVRRQTGGRGRGGNVWHSPKGCLTYSLLLSKEhpnVDPSVLEFYVLELVLAVLEALGL 84

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*....
gi 2623050    104 ----ECGLDVGIKWPNDILIGDKKVCGILTEAHARfNTLEYVVVGVGID 148
Cdd:pfam03099  85 ykpgISGIPCFVKWPNDLYVNGRKLAGILQRSTRG-GTLHHGVIGLGVN 132
PRK06955 PRK06955
biotin--[acetyl-CoA-carboxylase] ligase;
25-257 6.45e-19

biotin--[acetyl-CoA-carboxylase] ligase;


Pssm-ID: 235896 [Multi-domain]  Cd Length: 300  Bit Score: 84.06  E-value: 6.45e-19
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2623050    25 DEVDSTNnvAKRMA-------EDGATEGTVV-IAKTQSRGRGRRGKPWISpQGGIWMS-----IILRPevhPSRAPLLTL 91
Cdd:PRK06955  39 EETGSTN--ADLMArlkalprSADALPAPIVrVAYEQTAGRGRQGRPWFA-QPGNALLfsvacVLPRP---VAALAGLSL 112

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2623050    92 VAGVAVARTLRD---ECGLDVGIKWPNDILIGDKKVCGILTEahARFNTLEYVVVGVGIDTNV----DISHFPDDLREGA 164
Cdd:PRK06955 113 AVGVALAEALAAlpaALGQRIALKWPNDLLIAGRKLAGILIE--TVWATPDATAVVIGIGLNVrradAVAAEVDALRARE 190

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2623050   165 TSIKNEL-----KRDIKSSEL---IARFLRNFEETYNSFKDGKMDEILTEWRKLSKTIGRRVEIRKQLGEIVHGDAVGIN 236
Cdd:PRK06955 191 AALARGLppvalAAACAGANLtdtLAAALNALAPALQAFGADGLAPFAARWHALHAYAGREVVLLEDGAELARGVAHGID 270

                        250       260
                 ....*....|....*....|.
gi 2623050   237 SEGALILELDDGtLRKVISGE 257
Cdd:PRK06955 271 ETGQLLLDTPAG-RQAIAAGD 290
PRK08477 PRK08477
biotin--[acetyl-CoA-carboxylase] ligase;
20-190 4.51e-16

biotin--[acetyl-CoA-carboxylase] ligase;


Pssm-ID: 236273 [Multi-domain]  Cd Length: 211  Bit Score: 74.61  E-value: 4.51e-16
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2623050    20 EIKCFDEVDSTNNVAK-RMAEDGATEGTVVIAKTQSRGRGRRGKPWISPQGGIWMSIILRPEVHPSRAPL--LTLVAGVA 96
Cdd:PRK08477   2 EIRVFESLDSTQTYLIeKIKNGELKAPFAIVAKEQTAGIGSRGNSWEGKKGNLFFSFALKESDLPKDLPLqsSSIYFGFL 81

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2623050    97 VARTLRdECGLDVGIKWPNDILIGDKKVCGILTEAharfnTLEYVVVGVGIDTNvdishfpdDLREGATSIKNELKRDik 176
Cdd:PRK08477  82 LKEVLK-ELGSKVWLKWPNDLYLDDKKIGGVITNK-----IKNFIVCGIGLNLK--------FSPKNFACLDIEISDD-- 145

                        170
                 ....*....|....
gi 2623050   177 ssELIARFLRNFEE 190
Cdd:PRK08477 146 --LLLEGFLQKIEK 157
PRK05935 PRK05935
biotin--protein ligase; Provisional
 17-183 3.73e-13

biotin--protein ligase; Provisional


Pssm-ID: 235649 [Multi-domain]  Cd Length: 190  Bit Score: 65.99  E-value: 3.73e-13
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2623050    17 IGHEIKcfdEVDSTNNVAKR-MAEDGATEGTVVIAKTQSRGRGRRGKPWISPQGGIWMSIILRPEVHPSRAPLLTLVAGV 95
Cdd:PRK05935   4 IYYEIA---ETPSTNTTAKEgMHLWDPYALTVISTREQTAGKGKFGKSWHSSDQDLLASFCFFITVLNIDVSLLFRLGTE 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2623050    96 AVARTLRDECGLDVGIKWPNDILIGDKKVCGILTEAhARFNTLEYVVVGVGIDTNVDISHFpDDLREGATSIKNELKRDI 175
Cdd:PRK05935  81 AVMRLGEDLGITEAVIKWPNDVLVHGEKLCGVLCET-IPVKGGLGVILGIGVNGNTTKDEL-LGIDQPATSLQELLGHPI 158


                 ....*...
gi 2623050   176 KSSELIAR 183
Cdd:PRK05935 159 DLEEQRER 166
PRK13325 PRK13325
bifunctional biotin--[acetyl-CoA-carboxylase] ligase/type III pantothenate kinase;
36-257 3.74e-13

bifunctional biotin--[acetyl-CoA-carboxylase] ligase/type III pantothenate kinase;


Pssm-ID: 183976 [Multi-domain]  Cd Length: 592  Bit Score: 68.58  E-value: 3.74e-13
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2623050    36 RMAEDGATEgTVVIAKTQSRGRGRRGKPWISPQGGIWMS----IILRPEVHPSRaplLTLVAGVAVARTLrdEC-GLDVG 110
Cdd:PRK13325 102 RIAPDKAHK-TICVTHLQSKGRGRQGRKWSHRLGECLMFsfgwVFDRPQYELGS---LSPVAAVACRRAL--SRlGLKTQ 175

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2623050   111 IKWPNDILIGDKKVCGILTEAhARFNTLEYVVVGVGIDTNVdishfPDDLrEGATSIKNELKR-----DIKSSELIARFL 185
Cdd:PRK13325 176 IKWPNDLVVGRDKLGGILIET-VRTGGKTVAVVGIGINFVL-----PKEV-ENAASVQSLFQTasrrgNADAAVLLETLL 248

                        170       180       190       200       210       220       230
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 2623050   186 RNFEETYNSFKDGKMDEILTEWRKLSKTIGRRVEIRKQLGEIVHGDAVGINSEGALILELDDGTlRKVISGE 257
Cdd:PRK13325 249 AELDAVLLQYARDGFAPFVAEYQAANRDHGKAVLLLRDGETVFEGTVKGVDGQGVLHLETAEGK-QTVVSGE 319
PTZ00275 PTZ00275
biotin-acetyl-CoA-carboxylase ligase; Provisional
 24-256 5.43e-13

biotin-acetyl-CoA-carboxylase ligase; Provisional


Pssm-ID: 185536 [Multi-domain]  Cd Length: 285  Bit Score: 67.15  E-value: 5.43e-13
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2623050    24 FDEVDSTNNVAKR-MA---EDGA--TEGTVVIA-KTQSRGRGRRG------KPWISPQGGIWMSIIL---RPEVhpSRAP 87
Cdd:PTZ00275  22 FDVLDSTQLYCKRnMKrfiQNGKlqDDNMIIVScNEQTNGIGTRDtkknqdRIWLSEKGNLFTTFVFlwnRNDI--EKVK 99

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2623050    88 LLTLVAGVAVARTLrDECGLDVGIKWPNDILIGDKKVCGILTEAH--ARFNTL--EYVVVGVGIDTNVDISHFPDDLREG 163
Cdd:PTZ00275 100 YLAQTCTVAISKTL-EYFHLVTQIKWINDVLVNYKKIAGCLVHLYylDDFPNLnsRYVCVMVGIGINVTLEDKHNLLNNN 178

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2623050   164 ATSIKNELKRDIKSSELIA-------RFLRNFEETYNSFKDGKMDEIL--TEWRKLSKTigRRVEIrKQLGEIVHGDAVG 234
Cdd:PTZ00275 179 YTSIKKELQRDFNTPKSIPsveqvteKLIINLKAVINKLRKEGFSSFLdyITPRLLYKD--KKVLI-DQDNELIVGYLQG 255

                        250       260
                 ....*....|....*....|..
gi 2623050   235 INSEGALILELDDGTLRKVISG 256
Cdd:PTZ00275 256 LLHDGSLLLLREKNKLVRVNTG 277
BPL_C pfam02237
Biotin protein ligase C terminal domain; The function of this structural domain is unknown. It ...
 213-261 1.43e-10

Biotin protein ligase C terminal domain; The function of this structural domain is unknown. It is found to the C terminus of the biotin protein ligase catalytic domain pfam01317.


Pssm-ID: 426672 [Multi-domain]  Cd Length: 48  Bit Score: 55.16  E-value: 1.43e-10
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....*....
gi 2623050    213 TIGRRVEIRKQlGEIVHGDAVGINSEGALILELDDGTLRKVISGECIHR 261
Cdd:pfam02237   1 TLGREVRVLLG-DGIVEGIAVGIDDDGALLLETDDGTIRDINSGEVSLR 48
BPL_LplA_LipB cd16435
biotin-lipoate ligase family; This family includes biotin protein ligase (BPL), ...
 57-188 2.70e-09

biotin-lipoate ligase family; This family includes biotin protein ligase (BPL), lipoate-protein ligase A (LplA) and octanoyl-[acyl carrier protein]-protein acyltransferase (LipB). Biotin is covalently attached at the active site of certain enzymes that transfer carbon dioxide from bicarbonate to organic acids to form cellular metabolites. Biotin protein ligase (BPL) is the enzyme responsible for attaching biotin to a specific lysine at the active site of biotin enzymes. Biotin attachment is a two step reaction that results in the formation of an amide linkage between the carboxyl group of biotin and the epsilon-amino group of the modified lysine. Lipoate-protein ligase A (LplA) catalyses the formation of an amide linkage between lipoic acid and a specific lysine residue in lipoate dependent enzymes.


Pssm-ID: 319740  Cd Length: 198  Bit Score: 55.62  E-value: 2.70e-09
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2623050   57 RGRRGKPWISPQGGIWMSIILRPEVHPSRAPLLTLVAgVAVARTLRDeCGLDVGIKW-PNDILIGDKKVCGILTEAharf 135
Cdd:cd16435  68 RNRGGRAVSHDPGQLVFSPVIGPNVEFMISKFNLIIE-EGIRDAIAD-FGQSAEVKWgRNDLWIDNRKVCGIAVRV---- 141

                        90       100       110       120       130
                ....*....|....*....|....*....|....*....|....*....|....*...
gi 2623050  136 nTLEYVVVGVGIDTNVDISHF----PDDLR-EGATSIKNELKRDIKSSELIARFLRNF 188
Cdd:cd16435 142 -VKEAIFHGIALNLNQDLENFteiiPCGYKpERVTSLSLELGRKVTVEQVLERVLAAF 198
LplA COG0095
Lipoate-protein ligase A [Coenzyme transport and metabolism]; Lipoate-protein ligase A is part ...
 96-213 3.82e-08

Lipoate-protein ligase A [Coenzyme transport and metabolism]; Lipoate-protein ligase A is part of the Pathway/BioSystem: Lipoate biosynthesis


Pssm-ID: 439865  Cd Length: 246  Bit Score: 52.54  E-value: 3.82e-08
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2623050   96 AVARTLRDeCGLDVGIKWPNDILIGDKKVCGIlteAHARFNtlEYVVVGVGIDTNVDI---------------SHFPDDL 160
Cdd:COG0095 108 PILEALRK-LGVDAEFSGRNDIVVDGRKISGN---AQRRRK--GAVLHHGTLLVDGDLeklakvlrvpyeklrDKGIKSV 181

                        90       100       110       120       130
                ....*....|....*....|....*....|....*....|....*....|....
gi 2623050  161 REGATSIKNELKRDIKSSELIARFLRNFEETYNSFKDGKM-DEILTEWRKLSKT 213
Cdd:COG0095 182 RSRVTNLSELLGTDITREEVKEALLEAFAEVLGVLEPGELtDEELEAAEELAEE 235
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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