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Conserved domains on  [gi|18490905|gb|AAH22487|]
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GCLC protein [Homo sapiens]

Protein Classification

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
GCS super family cl03873
Glutamate-cysteine ligase; This family represents the catalytic subunit of glutamate-cysteine ...
 236-252 2.48e-09

Glutamate-cysteine ligase; This family represents the catalytic subunit of glutamate-cysteine ligase (E.C. 6.3.2.2), also known as gamma-glutamylcysteine synthetase (GCS). This enzyme catalyzes the rate limiting step in the biosynthesis of glutathione. The eukaryotic enzyme is a dimer of a heavy chain and a light chain with all the catalytic activity exhibited by the heavy chain (this family).


The actual alignment was detected with superfamily member pfam03074:

Pssm-ID: 460796  Cd Length: 369  Bit Score: 56.77  E-value: 2.48e-09
                          10
                  ....*....|....*..
gi 18490905   236 IYMDAMGFGMGNCCLQV 252
Cdd:pfam03074   1 IYMDAMGFGMGCCCLQV 17
PRK00087 super family cl35061
bifunctional 4-hydroxy-3-methylbut-2-enyl diphosphate reductase/30S ribosomal protein S1;
22-76 9.98e-04

bifunctional 4-hydroxy-3-methylbut-2-enyl diphosphate reductase/30S ribosomal protein S1;


The actual alignment was detected with superfamily member PRK00087:

Pssm-ID: 234623 [Multi-domain]  Cd Length: 647  Bit Score: 39.93  E-value: 9.98e-04
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*....
gi 18490905   22 VRRHGILQFLHI----YHAVKdRHKDVLKWGDEVEYMLVSFDHENKKVRlvLSGEKVLE 76
Cdd:PRK00087 495 VDIGGVDGLLHVseisWGRVE-KPSDVLKVGDEIKVYILDIDKENKKLS--LSLKKLLP 550
 
Name Accession Description Interval E-value
GCS pfam03074
Glutamate-cysteine ligase; This family represents the catalytic subunit of glutamate-cysteine ...
 236-252 2.48e-09

Glutamate-cysteine ligase; This family represents the catalytic subunit of glutamate-cysteine ligase (E.C. 6.3.2.2), also known as gamma-glutamylcysteine synthetase (GCS). This enzyme catalyzes the rate limiting step in the biosynthesis of glutathione. The eukaryotic enzyme is a dimer of a heavy chain and a light chain with all the catalytic activity exhibited by the heavy chain (this family).


Pssm-ID: 460796  Cd Length: 369  Bit Score: 56.77  E-value: 2.48e-09
                          10
                  ....*....|....*..
gi 18490905   236 IYMDAMGFGMGNCCLQV 252
Cdd:pfam03074   1 IYMDAMGFGMGCCCLQV 17
PRK00087 PRK00087
bifunctional 4-hydroxy-3-methylbut-2-enyl diphosphate reductase/30S ribosomal protein S1;
22-76 9.98e-04

bifunctional 4-hydroxy-3-methylbut-2-enyl diphosphate reductase/30S ribosomal protein S1;


Pssm-ID: 234623 [Multi-domain]  Cd Length: 647  Bit Score: 39.93  E-value: 9.98e-04
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*....
gi 18490905   22 VRRHGILQFLHI----YHAVKdRHKDVLKWGDEVEYMLVSFDHENKKVRlvLSGEKVLE 76
Cdd:PRK00087 495 VDIGGVDGLLHVseisWGRVE-KPSDVLKVGDEIKVYILDIDKENKKLS--LSLKKLLP 550
S1_RPS1_repeat_ec3 cd05688
S1_RPS1_repeat_ec3: Ribosomal protein S1 (RPS1) domain. RPS1 is a component of the small ...
 25-67 7.84e-03

S1_RPS1_repeat_ec3: Ribosomal protein S1 (RPS1) domain. RPS1 is a component of the small ribosomal subunit thought to be involved in the recognition and binding of mRNA's during translation initiation. The bacterial RPS1 domain architecture consists of 4-6 tandem S1 domains. In some bacteria, the tandem S1 array is located C-terminal to a 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (HMBPP reductase) domain. While RPS1 is found primarily in bacteria, proteins with tandem RPS1-like domains have been identified in plants and humans, however these lack the N-terminal HMBPP reductase domain. This CD includes S1 repeat 3 (ec3) of the Escherichia coli RPS1. Autoantibodies to double-stranded DNA from patients with systemic lupus erythematosus cross-react with the human RPS1 homolog.


Pssm-ID: 240193 [Multi-domain]  Cd Length: 68  Bit Score: 34.14  E-value: 7.84e-03
                        10        20        30        40
                ....*....|....*....|....*....|....*....|....*..
gi 18490905  25 HGILQFLHI----YHAVKdRHKDVLKWGDEVEYMLVSFDHENKKVRL 67
Cdd:cd05688  22 GGVDGLLHIsdmsWGRVK-HPSEVVNVGDEVEVKVLKIDKERKRISL 67
 
Name Accession Description Interval E-value
GCS pfam03074
Glutamate-cysteine ligase; This family represents the catalytic subunit of glutamate-cysteine ...
 236-252 2.48e-09

Glutamate-cysteine ligase; This family represents the catalytic subunit of glutamate-cysteine ligase (E.C. 6.3.2.2), also known as gamma-glutamylcysteine synthetase (GCS). This enzyme catalyzes the rate limiting step in the biosynthesis of glutathione. The eukaryotic enzyme is a dimer of a heavy chain and a light chain with all the catalytic activity exhibited by the heavy chain (this family).


Pssm-ID: 460796  Cd Length: 369  Bit Score: 56.77  E-value: 2.48e-09
                          10
                  ....*....|....*..
gi 18490905   236 IYMDAMGFGMGNCCLQV 252
Cdd:pfam03074   1 IYMDAMGFGMGCCCLQV 17
PRK00087 PRK00087
bifunctional 4-hydroxy-3-methylbut-2-enyl diphosphate reductase/30S ribosomal protein S1;
22-76 9.98e-04

bifunctional 4-hydroxy-3-methylbut-2-enyl diphosphate reductase/30S ribosomal protein S1;


Pssm-ID: 234623 [Multi-domain]  Cd Length: 647  Bit Score: 39.93  E-value: 9.98e-04
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*....
gi 18490905   22 VRRHGILQFLHI----YHAVKdRHKDVLKWGDEVEYMLVSFDHENKKVRlvLSGEKVLE 76
Cdd:PRK00087 495 VDIGGVDGLLHVseisWGRVE-KPSDVLKVGDEIKVYILDIDKENKKLS--LSLKKLLP 550
rpsA PRK06299
30S ribosomal protein S1; Reviewed
 26-67 1.47e-03

30S ribosomal protein S1; Reviewed


Pssm-ID: 235775 [Multi-domain]  Cd Length: 565  Bit Score: 39.38  E-value: 1.47e-03
                         10        20        30        40
                 ....*....|....*....|....*....|....*....|....*
gi 18490905   26 GILQFLHIYHAVKDRHKD---VLKWGDEVEYMLVSFDHENKKVRL 67
Cdd:PRK06299 483 GVEGLIRASELSRDRVEDateVLKVGDEVEAKVINIDRKNRRISL 527
S1_RPS1_repeat_ec3 cd05688
S1_RPS1_repeat_ec3: Ribosomal protein S1 (RPS1) domain. RPS1 is a component of the small ...
 25-67 7.84e-03

S1_RPS1_repeat_ec3: Ribosomal protein S1 (RPS1) domain. RPS1 is a component of the small ribosomal subunit thought to be involved in the recognition and binding of mRNA's during translation initiation. The bacterial RPS1 domain architecture consists of 4-6 tandem S1 domains. In some bacteria, the tandem S1 array is located C-terminal to a 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (HMBPP reductase) domain. While RPS1 is found primarily in bacteria, proteins with tandem RPS1-like domains have been identified in plants and humans, however these lack the N-terminal HMBPP reductase domain. This CD includes S1 repeat 3 (ec3) of the Escherichia coli RPS1. Autoantibodies to double-stranded DNA from patients with systemic lupus erythematosus cross-react with the human RPS1 homolog.


Pssm-ID: 240193 [Multi-domain]  Cd Length: 68  Bit Score: 34.14  E-value: 7.84e-03
                        10        20        30        40
                ....*....|....*....|....*....|....*....|....*..
gi 18490905  25 HGILQFLHI----YHAVKdRHKDVLKWGDEVEYMLVSFDHENKKVRL 67
Cdd:cd05688  22 GGVDGLLHIsdmsWGRVK-HPSEVVNVGDEVEVKVLKIDKERKRISL 67
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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