NCBI Home Page NCBI Site Search page NCBI Guide that lists and describes the NCBI resources
Conserved domains on  [gi|62132962|gb|AAH92149|]
View 

Pik3r4 protein, partial [Mus musculus]

Protein Classification

WD40 repeat domain-containing protein( domain architecture ID 1903264)

WD40 repeat domain-containing protein folds into a beta-propeller structure and functions as a scaffold, providing a platform for the interaction and assembly of several proteins into a signalosome; similar to a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly

CATH:  2.130.10.10
Gene Ontology:  GO:0005515
PubMed:  30069656|29026209
SCOP:  4002744

Graphical summary

 Zoom to residue level

show extra options »

Show site features     Horizontal zoom: ×

List of domain hits

 Name Accession Description Interval E-value
WD40 super family cl43672
WD40 repeat [General function prediction only];
16-155 6.45e-08

WD40 repeat [General function prediction only];


The actual alignment was detected with superfamily member COG2319:

Pssm-ID: 441893 [Multi-domain]  Cd Length: 403  Bit Score: 50.29  E-value: 6.45e-08
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 62132962  16 GDRRLTLW-ASSAPPLSELQPSPHSVHGIYCSPaDGNpILLTAGSDMKIRFWDLVSPERSYVVAGSTGSP-SVSYYKKii 93
Cdd:COG2319 266 ADGTVRLWdLATGELLRTLTGHSGGVNSVAFSP-DGK-LLASGSDDGTVRLWDLATGKLLRTLTGHTGAVrSVAFSPD-- 341

                        90       100       110       120       130       140
                ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 62132962  94 eGTEVVqeiqnkqkVGPSDDTPR----RGPESLPV--GHHDIITDIAtFQTTQGFIVTASRDGIVKVW 155
Cdd:COG2319 342 -GKTLA--------SGSDDGTVRlwdlATGELLRTltGHTGAVTSVA-FSPDGRTLASGSADGTVRLW 399
 
Name Accession Description Interval E-value
WD40 COG2319
WD40 repeat [General function prediction only];
16-155 6.45e-08

WD40 repeat [General function prediction only];


Pssm-ID: 441893 [Multi-domain]  Cd Length: 403  Bit Score: 50.29  E-value: 6.45e-08
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 62132962  16 GDRRLTLW-ASSAPPLSELQPSPHSVHGIYCSPaDGNpILLTAGSDMKIRFWDLVSPERSYVVAGSTGSP-SVSYYKKii 93
Cdd:COG2319 266 ADGTVRLWdLATGELLRTLTGHSGGVNSVAFSP-DGK-LLASGSDDGTVRLWDLATGKLLRTLTGHTGAVrSVAFSPD-- 341

                        90       100       110       120       130       140
                ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 62132962  94 eGTEVVqeiqnkqkVGPSDDTPR----RGPESLPV--GHHDIITDIAtFQTTQGFIVTASRDGIVKVW 155
Cdd:COG2319 342 -GKTLA--------SGSDDGTVRlwdlATGELLRTltGHTGAVTSVA-FSPDGRTLASGSADGTVRLW 399
WD40 cd00200
WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions ...
 4-155 4.49e-07

WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from its N-terminus and the WD dipeptide at its C-terminus and is 40 residues long, hence the name WD40; between GH and WD lies a conserved core; serves as a stable propeller-like platform to which proteins can bind either stably or reversibly; forms a propeller-like structure with several blades where each blade is composed of a four-stranded anti-parallel b-sheet; instances with few detectable copies are hypothesized to form larger structures by dimerization; each WD40 sequence repeat forms the first three strands of one blade and the last strand in the next blade; the last C-terminal WD40 repeat completes the blade structure of the first WD40 repeat to create the closed ring propeller-structure; residues on the top and bottom surface of the propeller are proposed to coordinate interactions with other proteins and/or small ligands; 7 copies of the repeat are present in this alignment.


Pssm-ID: 238121 [Multi-domain]  Cd Length: 289  Bit Score: 47.71  E-value: 4.49e-07
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 62132962   4 GNNEVSMWDMETGDRRLTLWASSapplselqpspHSVHGIYCSpADGNPiLLTAGSDMKIRFWDLVSPERSYVVAGSTG- 82
Cdd:cd00200  29 GDGTIKVWDLETGELLRTLKGHT-----------GPVRDVAAS-ADGTY-LASGSSDKTIRLWDLETGECVRTLTGHTSy 95

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 62132962  83 --SPSVSYYKKIIEGtevvqeiqnkqkvGPSD------DTPRRGPESLPVGHHDIITDIAtFQTTQGFIVTASRDGIVKV 154
Cdd:cd00200  96 vsSVAFSPDGRILSS-------------SSRDktikvwDVETGKCLTTLRGHTDWVNSVA-FSPDGTFVASSSQDGTIKL 161


                .
gi 62132962 155 W 155
Cdd:cd00200 162 W 162
WD40 smart00320
WD40 repeats; Note that these repeats are permuted with respect to the structural repeats ...
 125-156 1.09e-03

WD40 repeats; Note that these repeats are permuted with respect to the structural repeats (blades) of the beta propeller domain.


Pssm-ID: 197651 [Multi-domain]  Cd Length: 40  Bit Score: 34.98  E-value: 1.09e-03
                           10        20        30
                   ....*....|....*....|....*....|..
gi 62132962    125 GHHDIITDIAtFQTTQGFIVTASRDGIVKVWK 156
Cdd:smart00320  10 GHTGPVTSVA-FSPDGKYLASGSDDGTIKLWD 40
WD40 pfam00400
WD domain, G-beta repeat;
125-155 2.63e-03

WD domain, G-beta repeat;


Pssm-ID: 459801 [Multi-domain]  Cd Length: 39  Bit Score: 33.86  E-value: 2.63e-03
                          10        20        30
                  ....*....|....*....|....*....|.
gi 62132962   125 GHHDIITDIAtFQTTQGFIVTASRDGIVKVW 155
Cdd:pfam00400   9 GHTGSVTSLA-FSPDGKLLASGSDDGTVKVW 38
 
Name Accession Description Interval E-value
WD40 COG2319
WD40 repeat [General function prediction only];
16-155 6.45e-08

WD40 repeat [General function prediction only];


Pssm-ID: 441893 [Multi-domain]  Cd Length: 403  Bit Score: 50.29  E-value: 6.45e-08
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 62132962  16 GDRRLTLW-ASSAPPLSELQPSPHSVHGIYCSPaDGNpILLTAGSDMKIRFWDLVSPERSYVVAGSTGSP-SVSYYKKii 93
Cdd:COG2319 266 ADGTVRLWdLATGELLRTLTGHSGGVNSVAFSP-DGK-LLASGSDDGTVRLWDLATGKLLRTLTGHTGAVrSVAFSPD-- 341

                        90       100       110       120       130       140
                ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 62132962  94 eGTEVVqeiqnkqkVGPSDDTPR----RGPESLPV--GHHDIITDIAtFQTTQGFIVTASRDGIVKVW 155
Cdd:COG2319 342 -GKTLA--------SGSDDGTVRlwdlATGELLRTltGHTGAVTSVA-FSPDGRTLASGSADGTVRLW 399
WD40 COG2319
WD40 repeat [General function prediction only];
16-155 3.25e-07

WD40 repeat [General function prediction only];


Pssm-ID: 441893 [Multi-domain]  Cd Length: 403  Bit Score: 48.37  E-value: 3.25e-07
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 62132962  16 GDRRLTLW-ASSAPPLSELQPSPHSVHGIYCSPaDGNpILLTAGSDMKIRFWDLVSPERSYVVAGSTG-------SP--- 84
Cdd:COG2319 140 ADGTVRLWdLATGKLLRTLTGHSGAVTSVAFSP-DGK-LLASGSDDGTVRLWDLATGKLLRTLTGHTGavrsvafSPdgk 217

                        90       100       110       120       130       140       150
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 62132962  85 ---SVSYYKKI----IEGTEVVQEIQnkqkvgpsddtprrgpeslpvGHHDIITDIAtFQTTQGFIVTASRDGIVKVW 155
Cdd:COG2319 218 llaSGSADGTVrlwdLATGKLLRTLT---------------------GHSGSVRSVA-FSPDGRLLASGSADGTVRLW 273
WD40 cd00200
WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions ...
 4-155 4.49e-07

WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from its N-terminus and the WD dipeptide at its C-terminus and is 40 residues long, hence the name WD40; between GH and WD lies a conserved core; serves as a stable propeller-like platform to which proteins can bind either stably or reversibly; forms a propeller-like structure with several blades where each blade is composed of a four-stranded anti-parallel b-sheet; instances with few detectable copies are hypothesized to form larger structures by dimerization; each WD40 sequence repeat forms the first three strands of one blade and the last strand in the next blade; the last C-terminal WD40 repeat completes the blade structure of the first WD40 repeat to create the closed ring propeller-structure; residues on the top and bottom surface of the propeller are proposed to coordinate interactions with other proteins and/or small ligands; 7 copies of the repeat are present in this alignment.


Pssm-ID: 238121 [Multi-domain]  Cd Length: 289  Bit Score: 47.71  E-value: 4.49e-07
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 62132962   4 GNNEVSMWDMETGDRRLTLWASSapplselqpspHSVHGIYCSpADGNPiLLTAGSDMKIRFWDLVSPERSYVVAGSTG- 82
Cdd:cd00200  29 GDGTIKVWDLETGELLRTLKGHT-----------GPVRDVAAS-ADGTY-LASGSSDKTIRLWDLETGECVRTLTGHTSy 95

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 62132962  83 --SPSVSYYKKIIEGtevvqeiqnkqkvGPSD------DTPRRGPESLPVGHHDIITDIAtFQTTQGFIVTASRDGIVKV 154
Cdd:cd00200  96 vsSVAFSPDGRILSS-------------SSRDktikvwDVETGKCLTTLRGHTDWVNSVA-FSPDGTFVASSSQDGTIKL 161


                .
gi 62132962 155 W 155
Cdd:cd00200 162 W 162
WD40 COG2319
WD40 repeat [General function prediction only];
16-155 5.81e-07

WD40 repeat [General function prediction only];


Pssm-ID: 441893 [Multi-domain]  Cd Length: 403  Bit Score: 47.60  E-value: 5.81e-07
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 62132962  16 GDRRLTLW-ASSAPPLSELQPSPHSVHGIYCSPaDGNpILLTAGSDMKIRFWDLVSPERSYVVAGSTGSP-SVSYYKkii 93
Cdd:COG2319 224 ADGTVRLWdLATGKLLRTLTGHSGSVRSVAFSP-DGR-LLASGSADGTVRLWDLATGELLRTLTGHSGGVnSVAFSP--- 298

                        90       100       110       120       130       140
                ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 62132962  94 EGTEVVqeiqnkqkVGPSDDTPR----RGPESLPV--GHHDIITDIAtFQTTQGFIVTASRDGIVKVW 155
Cdd:COG2319 299 DGKLLA--------SGSDDGTVRlwdlATGKLLRTltGHTGAVRSVA-FSPDGKTLASGSDDGTVRLW 357
WD40 COG2319
WD40 repeat [General function prediction only];
16-155 4.51e-05

WD40 repeat [General function prediction only];


Pssm-ID: 441893 [Multi-domain]  Cd Length: 403  Bit Score: 42.21  E-value: 4.51e-05
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 62132962  16 GDRRLTLW-ASSAPPLSELQPSPHSVHGIYCSPaDGNpILLTAGSDMKIRFWDLVSPERSYVVAGSTG-------SP--- 84
Cdd:COG2319  98 ADGTVRLWdLATGLLLRTLTGHTGAVRSVAFSP-DGK-TLASGSADGTVRLWDLATGKLLRTLTGHSGavtsvafSPdgk 175

                        90       100       110       120       130       140       150
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 62132962  85 ---SVSYYKKI----IEGTEVVQEIQnkqkvgpsddtprrgpeslpvGHHDIITDIAtFQTTQGFIVTASRDGIVKVW 155
Cdd:COG2319 176 llaSGSDDGTVrlwdLATGKLLRTLT---------------------GHTGAVRSVA-FSPDGKLLASGSADGTVRLW 231
WD40 cd00200
WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions ...
 5-156 1.08e-04

WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from its N-terminus and the WD dipeptide at its C-terminus and is 40 residues long, hence the name WD40; between GH and WD lies a conserved core; serves as a stable propeller-like platform to which proteins can bind either stably or reversibly; forms a propeller-like structure with several blades where each blade is composed of a four-stranded anti-parallel b-sheet; instances with few detectable copies are hypothesized to form larger structures by dimerization; each WD40 sequence repeat forms the first three strands of one blade and the last strand in the next blade; the last C-terminal WD40 repeat completes the blade structure of the first WD40 repeat to create the closed ring propeller-structure; residues on the top and bottom surface of the propeller are proposed to coordinate interactions with other proteins and/or small ligands; 7 copies of the repeat are present in this alignment.


Pssm-ID: 238121 [Multi-domain]  Cd Length: 289  Bit Score: 40.78  E-value: 1.08e-04
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 62132962   5 NNEVSMWDMETGDRRLTLwassapplselqpSPH--SVHGIYCSPaDGNPiLLTAGSDMKIRFWDL-------------- 68
Cdd:cd00200 156 DGTIKLWDLRTGKCVATL-------------TGHtgEVNSVAFSP-DGEK-LLSSSSDGTIKLWDLstgkclgtlrghen 220

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 62132962  69 ------VSPERSYVVAGSTGSpSVSYYKkiIEGTEVVQEIQnkqkvgpsddtprrgpeslpvGHHDIITDIAtFQTTQGF 142
Cdd:cd00200 221 gvnsvaFSPDGYLLASGSEDG-TIRVWD--LRTGECVQTLS---------------------GHTNSVTSLA-WSPDGKR 275

                       170
                ....*....|....
gi 62132962 143 IVTASRDGIVKVWK 156
Cdd:cd00200 276 LASGSADGTIRIWD 289
WD40 smart00320
WD40 repeats; Note that these repeats are permuted with respect to the structural repeats ...
 125-156 1.09e-03

WD40 repeats; Note that these repeats are permuted with respect to the structural repeats (blades) of the beta propeller domain.


Pssm-ID: 197651 [Multi-domain]  Cd Length: 40  Bit Score: 34.98  E-value: 1.09e-03
                           10        20        30
                   ....*....|....*....|....*....|..
gi 62132962    125 GHHDIITDIAtFQTTQGFIVTASRDGIVKVWK 156
Cdd:smart00320  10 GHTGPVTSVA-FSPDGKYLASGSDDGTIKLWD 40
WD40 cd00200
WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions ...
 125-156 2.58e-03

WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from its N-terminus and the WD dipeptide at its C-terminus and is 40 residues long, hence the name WD40; between GH and WD lies a conserved core; serves as a stable propeller-like platform to which proteins can bind either stably or reversibly; forms a propeller-like structure with several blades where each blade is composed of a four-stranded anti-parallel b-sheet; instances with few detectable copies are hypothesized to form larger structures by dimerization; each WD40 sequence repeat forms the first three strands of one blade and the last strand in the next blade; the last C-terminal WD40 repeat completes the blade structure of the first WD40 repeat to create the closed ring propeller-structure; residues on the top and bottom surface of the propeller are proposed to coordinate interactions with other proteins and/or small ligands; 7 copies of the repeat are present in this alignment.


Pssm-ID: 238121 [Multi-domain]  Cd Length: 289  Bit Score: 36.93  E-value: 2.58e-03
                        10        20        30
                ....*....|....*....|....*....|..
gi 62132962 125 GHHDIITDIAtFQTTQGFIVTASRDGIVKVWK 156
Cdd:cd00200   7 GHTGGVTCVA-FSPDGKLLATGSGDGTIKVWD 37
WD40 pfam00400
WD domain, G-beta repeat;
125-155 2.63e-03

WD domain, G-beta repeat;


Pssm-ID: 459801 [Multi-domain]  Cd Length: 39  Bit Score: 33.86  E-value: 2.63e-03
                          10        20        30
                  ....*....|....*....|....*....|.
gi 62132962   125 GHHDIITDIAtFQTTQGFIVTASRDGIVKVW 155
Cdd:pfam00400   9 GHTGSVTSLA-FSPDGKLLASGSDDGTVKVW 38
WD40 cd00200
WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions ...
 5-155 8.74e-03

WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from its N-terminus and the WD dipeptide at its C-terminus and is 40 residues long, hence the name WD40; between GH and WD lies a conserved core; serves as a stable propeller-like platform to which proteins can bind either stably or reversibly; forms a propeller-like structure with several blades where each blade is composed of a four-stranded anti-parallel b-sheet; instances with few detectable copies are hypothesized to form larger structures by dimerization; each WD40 sequence repeat forms the first three strands of one blade and the last strand in the next blade; the last C-terminal WD40 repeat completes the blade structure of the first WD40 repeat to create the closed ring propeller-structure; residues on the top and bottom surface of the propeller are proposed to coordinate interactions with other proteins and/or small ligands; 7 copies of the repeat are present in this alignment.


Pssm-ID: 238121 [Multi-domain]  Cd Length: 289  Bit Score: 35.39  E-value: 8.74e-03
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 62132962   5 NNEVSMWDMETGDRRLTLwassapplselqpSPHSvHGIYCSPADGNPILLTAGS-DMKIRFWDLVSPERSYVVAGSTGS 83
Cdd:cd00200 114 DKTIKVWDVETGKCLTTL-------------RGHT-DWVNSVAFSPDGTFVASSSqDGTIKLWDLRTGKCVATLTGHTGE 179

                        90       100       110       120       130       140       150
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 62132962  84 -PSVSYYKKiieGTEVVqeiqnkqkVGPSDDT-----PRRGPES-LPVGHHDIITDIAtFQTTQGFIVTASRDGIVKVW 155
Cdd:cd00200 180 vNSVAFSPD---GEKLL--------SSSSDGTiklwdLSTGKCLgTLRGHENGVNSVA-FSPDGYLLASGSEDGTIRVW 246
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
Help | Disclaimer | Write to the Help Desk
NCBI | NLM | NIH