GIY-YIG domain of hypothetical proteins from Metazoa; Members of this family are functionally uncharacterized hypothetical proteins from Metazoa. They have bacterial homologs that display sequence homology with the catalytic GIY-YIG domain of bacterial UvrC DNA repair proteins. However, unlike their bacterial relatives, these Metazoan proteins contain an N-terminal extension that includes the region of approximately 3-4 ankyrin repeats, unique motifs mediating protein-protein interactions. Some of them do have an additional LEM domain located between ankyrin repeats region and GIY-YIG domain. The LEM domain, found in inner nuclear membrane proteins, may be involved in protein- or DNA-binding. The different domains composition suggests members in this subfamily might participate in interactions with multiple partners and imply some important cellular functions.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 148877758  88 SFTYLLLDPRLTKDLPARASSLTLAECLQCFVRAIFYVGKGTRARPDAHLWEALGYHDQPR-KQVCPKVRRILDIWASGR 166
Cdd:cd10454    1 SFNYLLLDPRVTRNLPSRARGLTPIETFQTFVSSIFYVGKGKRSRPYAHFYEALKQHNDKDkKKGSRKLRRILDIWNSGL 80

                         90       100       110
                 ....*....|....*....|....*....|....
gi 148877758 167 GIISLHCFQHVVAMEAYTREACLLDALGLQTLTN 200
Cdd:cd10454   81 GVVSLHCFQNVIPVEAYTREAAMIEALGLSNLTN 114

GIY-YIG domain of hypothetical proteins from Metazoa; Members of this family are functionally uncharacterized hypothetical proteins from Metazoa. They have bacterial homologs that display sequence homology with the catalytic GIY-YIG domain of bacterial UvrC DNA repair proteins. However, unlike their bacterial relatives, these Metazoan proteins contain an N-terminal extension that includes the region of approximately 3-4 ankyrin repeats, unique motifs mediating protein-protein interactions. Some of them do have an additional LEM domain located between ankyrin repeats region and GIY-YIG domain. The LEM domain, found in inner nuclear membrane proteins, may be involved in protein- or DNA-binding. The different domains composition suggests members in this subfamily might participate in interactions with multiple partners and imply some important cellular functions.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 148877758  88 SFTYLLLDPRLTKDLPARASSLTLAECLQCFVRAIFYVGKGTRARPDAHLWEALGYHDQPR-KQVCPKVRRILDIWASGR 166
Cdd:cd10454    1 SFNYLLLDPRVTRNLPSRARGLTPIETFQTFVSSIFYVGKGKRSRPYAHFYEALKQHNDKDkKKGSRKLRRILDIWNSGL 80

                         90       100       110
                 ....*....|....*....|....*....|....
gi 148877758 167 GIISLHCFQHVVAMEAYTREACLLDALGLQTLTN 200
Cdd:cd10454   81 GVVSLHCFQNVIPVEAYTREAAMIEALGLSNLTN 114

LEM (Lap2/Emerin/Man1) domain found in ankyrin repeat and LEM domain-containing protein 1 (ANKL1); The family includes ANKL1, also termed ankyrin repeat domain-containing protein 41 (ANKRD41), or LEM-domain containing protein 3 (LEM3), and similar proteins. Although their biological roles remain unclear, the family members contain an N-terminal ankyrin repeat region, LEM domain and C-terminal GIY-YIG nuclease domain. The ankyrin repeats are unique motifs mediating protein-protein interactions. The LEM domain, mainly found in inner nuclear membrane proteins, may be involved in protein- or DNA-binding.

                         10        20        30
                 ....*....|....*....|....*....|....*...
gi 148877758   1 MSDLQLLQALRALGYSPGPVTPFTRGHYLRRLQEAQAS 38
Cdd:cd12943    1 LSDLDLLRGLRALGESPGPITPFTRRVYLRRLEELQRA 38

GIY-YIG domain of hypothetical proteins from Metazoa; Members of this family are functionally uncharacterized hypothetical proteins from Metazoa. They have bacterial homologs that display sequence homology with the catalytic GIY-YIG domain of bacterial UvrC DNA repair proteins. However, unlike their bacterial relatives, these Metazoan proteins contain an N-terminal extension that includes the region of approximately 3-4 ankyrin repeats, unique motifs mediating protein-protein interactions. Some of them do have an additional LEM domain located between ankyrin repeats region and GIY-YIG domain. The LEM domain, found in inner nuclear membrane proteins, may be involved in protein- or DNA-binding. The different domains composition suggests members in this subfamily might participate in interactions with multiple partners and imply some important cellular functions.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 148877758  88 SFTYLLLDPRLTKDLPARASSLTLAECLQCFVRAIFYVGKGTRARPDAHLWEALGYHDQPR-KQVCPKVRRILDIWASGR 166
Cdd:cd10454    1 SFNYLLLDPRVTRNLPSRARGLTPIETFQTFVSSIFYVGKGKRSRPYAHFYEALKQHNDKDkKKGSRKLRRILDIWNSGL 80

                         90       100       110
                 ....*....|....*....|....*....|....
gi 148877758 167 GIISLHCFQHVVAMEAYTREACLLDALGLQTLTN 200
Cdd:cd10454   81 GVVSLHCFQNVIPVEAYTREAAMIEALGLSNLTN 114

Uncharacterized protein, contains GIY-YIG domain [Function unknown];

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 148877758  91 YLLLDPRltkdlparassltlaeclqcfVRAIFYVGKGTRARPDAHLWEALGYHDQPRkqvcPKVRRILDIWASGRGIIS 170
Cdd:COG3680   18 YALIDPR---------------------DNKPFYIGKGKGNRVFAHLREAIASNESES----AKLERIREIKKAGLDVEH 72

                         90       100       110
                 ....*....|....*....|....*....|....*...
gi 148877758 171 LHCFQHVVAMEAYTREACLLDALGL--QTLTNQKQGHY 206
Cdd:COG3680   73 YILRHGLDEKTAFEVEAALIDLLGIveAKLTNIVRGHG 110

LEM (Lap2/Emerin/Man1) domain found in emerin, lamina-associated polypeptide 2 (LAP2), inner nuclear membrane protein Man1 and similar proteins; The family corresponds to a group of inner nuclear membrane proteins containing LEM domain. Emerin occurs in four phosphorylated forms and plays a role in cell cycle-dependent events. It is absent from the inner nuclear membrane in most patients with X-linked muscular dystrophy. Emerin interacts with A-type and B-type lamins. Man1, also termed LEM domain-containing protein 3 (LEMD3) is an integral protein of the inner nuclear membrane that binds to nuclear lamins and emerin, thus playing a role in nuclear organization. LAP2, also termed thymopoietin (TP), or thymopoietin-related peptide (TPRP), is composed of isoform alpha and isoforms beta/gamma and may be involved in chromatin organization and post-mitotic reassembly. Some LAP2 isoforms are inner nuclear membrane proteins that can bind to nuclear lamins and chromatin, while others are non-membrane nuclear polypeptides. This family also contains LEM domain-containing protein LEMP-1 and LEM2. LEMP-1, also termed cancer/testis antigen 50 (CT50), is encoded by LEMD1, a novel testis-specific gene expressed in colorectal cancers. LEMP-1 may function as a cancer-testis antigen for immunotherapy of colorectal carcinoma (CRC). LEM2, also termed LEMD2, is a novel Man1-related ubiquitously expressed inner nuclear membrane protein required for normal nuclear envelope morphology. Association with lamin A is required for its proper nuclear envelope localization while its binding to lamin C plays an important role in the organization of lamin A/C complexes. Some uncharacterized LEM domain-containing proteins are also included in this family. Unlike other family members, these harbor an ankyrin repeat region that may mediate protein-protein interactions.

                         10        20
                 ....*....|....*....|..
gi 148877758  14 GYSPGPVTPFTRGHYLRRLQEA 35
Cdd:cd12934   14 GEPPGPITDTTRKVYLKKLAKL 35