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Conserved domains on  [gi|20531755|gb|AAM27457|]
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IH1 [Mus musculus]

Protein Classification

RWD domain-containing protein( domain architecture ID 13715710)

RWD domain-containing protein similar to human RWD domain-containing protein 1 (RWDD1), also called DRG family-regulatory protein 2 (DFRP2), which protects DRG2 from proteolytic degradation

CATH:  3.10.110.10
Gene Ontology:  GO:0005515|GO:0002181
PubMed:  26276859|25918163

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
RWD_RWDD1 cd23816
RWD domain of RWD domain-containing protein 1 (RWDD1) and related proteins; RWDD1, also called ...
 4-119 1.71e-58

RWD domain of RWD domain-containing protein 1 (RWDD1) and related proteins; RWDD1, also called DRG family-regulatory protein 2 (DFRP2), or PTD013, interacts with DRG2 and protects DRG2 from proteolytic degradation. It is an androgen receptor-interacting protein that functions as a coactivator of androgen-dependent transcription.


:

Pssm-ID: 467652  Cd Length: 118  Bit Score: 180.95  E-value: 1.71e-58
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 20531755   4 YGEEQRNELEALESIYPDSFTVLSESPP-SFTITVTSEAGEN-DETVQTTLKFTYSEKYPDETPLYEIFSQENLEDNDVS 81
Cdd:cd23816   1 YKEEQRNELEALESIYPDEFTVLSEEPPiSFTITVTSEEEENeDETVSVTLKFTYTEKYPDEAPLIEIISHENLEDEDIE 80

                        90       100       110
                ....*....|....*....|....*....|....*...
gi 20531755  82 DILKLLALQAEENLGMVMIFTLVTAVQEKLNEIVDQIK 119
Cdd:cd23816  81 DLLELLEQQAEENLGMVMVFTIVSAVQEKLNEIVDQIK 118
DFRP_C super family cl37909
DRG Family Regulatory Proteins, Tma46; DFRP_C is a family of eukaryotic translation ...
 144-197 3.86e-04

DRG Family Regulatory Proteins, Tma46; DFRP_C is a family of eukaryotic translation machinery-associated protein 46 proteins that are the binding partner for the highly conserved Developmentally Regulated GTP-binding (DRG) GTPases. Thus this family is referred to as DRG Family Regulatory Proteins (DFRP). Binding of this DFRP modulates the function of the GTPase.


The actual alignment was detected with superfamily member pfam16543:

Pssm-ID: 465167  Cd Length: 89  Bit Score: 38.34  E-value: 3.86e-04
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....
gi 20531755   144 TPVTIENFLSWKAKfdaELLEIKKKRMKEEEQAGKNKLSGKQLFETDHNLDTSD 197
Cdd:pfam16543  20 TPVTPETFAKWKKK---RIDKKEAEEEAERKKKKADGLSGRELFEFNPDLFEDD 70
 
Name Accession Description Interval E-value
RWD_RWDD1 cd23816
RWD domain of RWD domain-containing protein 1 (RWDD1) and related proteins; RWDD1, also called ...
 4-119 1.71e-58

RWD domain of RWD domain-containing protein 1 (RWDD1) and related proteins; RWDD1, also called DRG family-regulatory protein 2 (DFRP2), or PTD013, interacts with DRG2 and protects DRG2 from proteolytic degradation. It is an androgen receptor-interacting protein that functions as a coactivator of androgen-dependent transcription.


Pssm-ID: 467652  Cd Length: 118  Bit Score: 180.95  E-value: 1.71e-58
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 20531755   4 YGEEQRNELEALESIYPDSFTVLSESPP-SFTITVTSEAGEN-DETVQTTLKFTYSEKYPDETPLYEIFSQENLEDNDVS 81
Cdd:cd23816   1 YKEEQRNELEALESIYPDEFTVLSEEPPiSFTITVTSEEEENeDETVSVTLKFTYTEKYPDEAPLIEIISHENLEDEDIE 80

                        90       100       110
                ....*....|....*....|....*....|....*...
gi 20531755  82 DILKLLALQAEENLGMVMIFTLVTAVQEKLNEIVDQIK 119
Cdd:cd23816  81 DLLELLEQQAEENLGMVMVFTIVSAVQEKLNEIVDQIK 118
RWD pfam05773
RWD domain; This domain was identified in WD40 repeat proteins and Ring finger domain proteins. ...
 6-111 1.13e-27

RWD domain; This domain was identified in WD40 repeat proteins and Ring finger domain proteins. The function of this domain is unknown. GCN2 is the alpha-subunit of the only translation initiation factor (eIF2 alpha) kinase that appears in all eukaryotes. Its function requires an interaction with GCN1 via the domain at its N-terminus, which is termed the RWD domain after three major RWD-containing proteins: RING finger-containing proteins, WD-repeat-containing proteins, and yeast DEAD (DEXD)-like helicases. The structure forms an alpha + beta sandwich fold consisting of two layers: a four-stranded antiparallel beta-sheet, and three side-by-side alpha-helices.


Pssm-ID: 399058  Cd Length: 111  Bit Score: 102.02  E-value: 1.13e-27
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 20531755     6 EEQRNELEALESIYPDSFTVLSESPP-----SFTITVTSEAGENDETVQTTLKFTYSEKYPDETPLYEIFSQENLEDNDV 80
Cdd:pfam05773   1 EEQEEELEALESIYPDEFEVISDSPYesleiEIKLSLDSDESDSSHLPPLVLKFTLPEDYPDEPPKISLSSPWNLSDEQV 80

                          90       100       110
                  ....*....|....*....|....*....|.
gi 20531755    81 SDILKLLALQAEENLGMVMIFTLVTAVQEKL 111
Cdd:pfam05773  81 LSLLEELEELAEENLGEVMIFELIEWLQENL 111
RWD smart00591
domain in RING finger and WD repeat containing proteins and DEXDc-like helicases subfamily ...
 11-114 1.04e-23

domain in RING finger and WD repeat containing proteins and DEXDc-like helicases subfamily related to the UBCc domain;


Pssm-ID: 214735  Cd Length: 107  Bit Score: 91.65  E-value: 1.04e-23
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 20531755     11 ELEALESIYPDSFTVLSESPPSFTITVT---SEAGENDETVQTTLKFTYSEKYPDETPLYEIFSQENLEDNDVSDILKLL 87
Cdd:smart00591   1 ELEALESIYPEDFEVIDEDARIPEITIKlspSSDEGEDQYVSLTLQVKLPENYPDEAPPISLLNSEGLSDEQLAELLKKL 80

                           90       100
                   ....*....|....*....|....*..
gi 20531755     88 ALQAEENLGMVMIFTLVTAVQEKLNEI 114
Cdd:smart00591  81 EEIAEENLGEVMIFELVEKLQEFLSEF 107
DFRP_C pfam16543
DRG Family Regulatory Proteins, Tma46; DFRP_C is a family of eukaryotic translation ...
 144-197 3.86e-04

DRG Family Regulatory Proteins, Tma46; DFRP_C is a family of eukaryotic translation machinery-associated protein 46 proteins that are the binding partner for the highly conserved Developmentally Regulated GTP-binding (DRG) GTPases. Thus this family is referred to as DRG Family Regulatory Proteins (DFRP). Binding of this DFRP modulates the function of the GTPase.


Pssm-ID: 465167  Cd Length: 89  Bit Score: 38.34  E-value: 3.86e-04
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....
gi 20531755   144 TPVTIENFLSWKAKfdaELLEIKKKRMKEEEQAGKNKLSGKQLFETDHNLDTSD 197
Cdd:pfam16543  20 TPVTPETFAKWKKK---RIDKKEAEEEAERKKKKADGLSGRELFEFNPDLFEDD 70
COG5252 COG5252
Uncharacterized conserved protein, contains CCCH-type Zn-finger protein [General function ...
 144-235 9.35e-04

Uncharacterized conserved protein, contains CCCH-type Zn-finger protein [General function prediction only];


Pssm-ID: 227577 [Multi-domain]  Cd Length: 299  Bit Score: 39.69  E-value: 9.35e-04
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 20531755 144 TPVTIENFLSWK-AKFDAELLEIKKKRMKEEEQAGKNKLSGKQLFETDHNLdtsdiqFLEDAGNNVEVDESLFQemDDLE 222
Cdd:COG5252 212 TPLTEENFKEWKdGRRLRILKQKEEKESARKVKGRATGTKGVELFETRRDL------FKDDVEAGEEWDYTERC--YSES 283

                        90
                ....*....|...
gi 20531755 223 LEDGEDDPDYNPV 235
Cdd:COG5252 284 EDEEGHDKATAPI 296
 
Name Accession Description Interval E-value
RWD_RWDD1 cd23816
RWD domain of RWD domain-containing protein 1 (RWDD1) and related proteins; RWDD1, also called ...
 4-119 1.71e-58

RWD domain of RWD domain-containing protein 1 (RWDD1) and related proteins; RWDD1, also called DRG family-regulatory protein 2 (DFRP2), or PTD013, interacts with DRG2 and protects DRG2 from proteolytic degradation. It is an androgen receptor-interacting protein that functions as a coactivator of androgen-dependent transcription.


Pssm-ID: 467652  Cd Length: 118  Bit Score: 180.95  E-value: 1.71e-58
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 20531755   4 YGEEQRNELEALESIYPDSFTVLSESPP-SFTITVTSEAGEN-DETVQTTLKFTYSEKYPDETPLYEIFSQENLEDNDVS 81
Cdd:cd23816   1 YKEEQRNELEALESIYPDEFTVLSEEPPiSFTITVTSEEEENeDETVSVTLKFTYTEKYPDEAPLIEIISHENLEDEDIE 80

                        90       100       110
                ....*....|....*....|....*....|....*...
gi 20531755  82 DILKLLALQAEENLGMVMIFTLVTAVQEKLNEIVDQIK 119
Cdd:cd23816  81 DLLELLEQQAEENLGMVMVFTIVSAVQEKLNEIVDQIK 118
RWD_GCN2 cd23823
RWD domain of eIF-2-alpha kinase GCN2 and related proteins; GCN2 (EC 2.7.11.1), also called ...
 6-114 4.52e-31

RWD domain of eIF-2-alpha kinase GCN2 and related proteins; GCN2 (EC 2.7.11.1), also called eukaryotic translation initiation factor 2-alpha kinase 4 (EIF2AK4), acts as a metabolic-stress sensing protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (EIF2S1/eIF-2-alpha) in response to low amino acid availability. It also plays a role in modulating the adaptive immune response to yellow fever virus infection and promotes dendritic cells to initiate autophagy and antigene presentation to both CD4(+) and CD8(+) T-cells under amino acid starvation.


Pssm-ID: 467659  Cd Length: 117  Bit Score: 110.77  E-value: 4.52e-31
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 20531755   6 EEQRNELEALESIYPDSFTVLSE-----SPPSFTITVTSEAGENDE-TVQTTLKFTYSEKYPDETPLYEIFSQENLEDND 79
Cdd:cd23823   2 EEQEEELEALQSIYGDDFEDLSSkkavwSPPEFRIRLRPQEGESEEnHVSVDLHVKFPPTYPDVPPEIELENVKGLSDEQ 81

                        90       100       110
                ....*....|....*....|....*....|....*
gi 20531755  80 VSDILKLLALQAEENLGMVMIFTLVTAVQEKLNEI 114
Cdd:cd23823  82 LEELLKELEELAKELLGEEMIFELAEAVQEFLEEH 116
RWD pfam05773
RWD domain; This domain was identified in WD40 repeat proteins and Ring finger domain proteins. ...
 6-111 1.13e-27

RWD domain; This domain was identified in WD40 repeat proteins and Ring finger domain proteins. The function of this domain is unknown. GCN2 is the alpha-subunit of the only translation initiation factor (eIF2 alpha) kinase that appears in all eukaryotes. Its function requires an interaction with GCN1 via the domain at its N-terminus, which is termed the RWD domain after three major RWD-containing proteins: RING finger-containing proteins, WD-repeat-containing proteins, and yeast DEAD (DEXD)-like helicases. The structure forms an alpha + beta sandwich fold consisting of two layers: a four-stranded antiparallel beta-sheet, and three side-by-side alpha-helices.


Pssm-ID: 399058  Cd Length: 111  Bit Score: 102.02  E-value: 1.13e-27
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 20531755     6 EEQRNELEALESIYPDSFTVLSESPP-----SFTITVTSEAGENDETVQTTLKFTYSEKYPDETPLYEIFSQENLEDNDV 80
Cdd:pfam05773   1 EEQEEELEALESIYPDEFEVISDSPYesleiEIKLSLDSDESDSSHLPPLVLKFTLPEDYPDEPPKISLSSPWNLSDEQV 80

                          90       100       110
                  ....*....|....*....|....*....|.
gi 20531755    81 SDILKLLALQAEENLGMVMIFTLVTAVQEKL 111
Cdd:pfam05773  81 LSLLEELEELAEENLGEVMIFELIEWLQENL 111
RWD smart00591
domain in RING finger and WD repeat containing proteins and DEXDc-like helicases subfamily ...
 11-114 1.04e-23

domain in RING finger and WD repeat containing proteins and DEXDc-like helicases subfamily related to the UBCc domain;


Pssm-ID: 214735  Cd Length: 107  Bit Score: 91.65  E-value: 1.04e-23
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 20531755     11 ELEALESIYPDSFTVLSESPPSFTITVT---SEAGENDETVQTTLKFTYSEKYPDETPLYEIFSQENLEDNDVSDILKLL 87
Cdd:smart00591   1 ELEALESIYPEDFEVIDEDARIPEITIKlspSSDEGEDQYVSLTLQVKLPENYPDEAPPISLLNSEGLSDEQLAELLKKL 80

                           90       100
                   ....*....|....*....|....*..
gi 20531755     88 ALQAEENLGMVMIFTLVTAVQEKLNEI 114
Cdd:smart00591  81 EEIAEENLGEVMIFELVEKLQEFLSEF 107
RWD_DRWD_ELF-like cd11605
RWD, DRWD, and ELF domain family; The family includes the RWD, double-RWD (DRWD), and ELF ...
 14-107 1.20e-19

RWD, DRWD, and ELF domain family; The family includes the RWD, double-RWD (DRWD), and ELF domains. They belong to the ubiquitin-conjugating (UBC) superfamily that represents a structural domain with an alpha-beta(4)-alpha(3) core fold. The RWD domain (named after three major RWD-containing proteins: RING finger, WD-repeat-containing proteins and DEXD-like helicases) mediates protein-protein interactions in a variety of pathways in eukaryotes. The DRWD domain is responsible for substrate binding. It is involved in interactions with other kinetochore proteins. The ELF (N-terminal E2-like fold) domain is found in all Fanconi anemia group L protein (FANCL) homologs. It is required to promote efficient DNA damage-induced FANCD2 (Fanconi anemia group D2 protein) monoubiquitination in vertebrate cells.


Pssm-ID: 467641  Cd Length: 94  Bit Score: 80.69  E-value: 1.20e-19
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 20531755  14 ALESIYPDSFTVLSES-PPSFTITVTSEAGENDETVqtTLKFTYSEKY-PDETPLYEIFSQENLEDNDVSDILKLLALQA 91
Cdd:cd11605   1 ALESIYGDELEVLSDDsPLRFSIRLSPEEEEDDPPL--ELEFTLPPGYpPEEPPLITLRSPKLSSAERLSLLKLELEEAA 78

                        90
                ....*....|....*.
gi 20531755  92 EENLGMVMIFTLVTAV 107
Cdd:cd11605  79 EENLGEPMLFDLVEAL 94
RWD_RNF25 cd23818
RWD domain of RING finger protein 25 (RNF25) and related proteins; RNF25 (EC 2.3.2.27), also ...
 11-111 1.27e-18

RWD domain of RING finger protein 25 (RNF25) and related proteins; RNF25 (EC 2.3.2.27), also known as AO7, is a putative E3 ubiquitin-protein ligase that was initially identified as an interacting protein of the E2 ubiquitin-conjugating enzyme, Ubc5B. It is ubiquitously expressed in various tissues and is predominantly localized in the nucleus. RNF25 activates nuclear factor (NF)-kappaB-dependent gene expression upon stimulation with interleukin-1 beta (IL-1beta), or tumor necrosis factor (TNF), or overexpression of NF-kappaB-inducing kinase. It interacts with the p65 transactivation domain (TAD) and modulates its transcriptional activity.


Pssm-ID: 467654  Cd Length: 109  Bit Score: 78.35  E-value: 1.27e-18
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 20531755  11 ELEALESIYPDSFTVLSE--SPPSFTITVTSEAGENDET--VQTTLKFTYSEKYPDETPLYEIFSQENLEDNDVSDILKL 86
Cdd:cd23818   4 ELEALEAIYPDELKVVSEdgAPTELSITLHPATADDESEqyVRLTLVITLPPGYPEEPPKISLRNPRGLSDARLARLLSL 83

                        90       100
                ....*....|....*....|....*
gi 20531755  87 LALQAEENLGMVMIFTLVTAVQEKL 111
Cdd:cd23818  84 LKELAEERAGEPMLFELIELAKEFL 108
RWD_IMPACT cd23821
RWD domain of protein IMPACT and related proteins; IMPACT, also imprinted and ancient gene ...
 9-111 1.72e-14

RWD domain of protein IMPACT and related proteins; IMPACT, also imprinted and ancient gene protein homolog, acts as a translational regulator that ensures constant high levels of translation upon a variety of stress conditions, such as amino acid starvation, UV-C irradiation, proteasome inhibitor treatment, and glucose deprivation. It plays a role as a negative regulator of EIF2AK4/GCN2 kinase activity. It impairs GCN1-mediated EIF2AK4/GCN2 activation, and hence EIF2AK4/GCN2-mediated eIF-2-alpha phosphorylation and subsequent down-regulation of protein synthesis. IMPACT may be required to regulate translation in specific neuronal cells under amino acid starvation conditions by preventing GCN2 activation and therefore ATF4 synthesis. Through its inhibitory action on EIF2AK4/GCN2, IMPACT plays a role in differentiation of neuronal cells by stimulating neurite outgrowth.


Pssm-ID: 467657  Cd Length: 101  Bit Score: 67.26  E-value: 1.72e-14
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 20531755   9 RNELEALESIYPDSFTVLSESPPSFTITVTSeagENDETVQTTLKFTYSEKYP-DETPLYEIfSQENLEDNDVSDILKLL 87
Cdd:cd23821   1 AEEIEALEAIYGEDFVVIDESARSFVIRIEL---DGPHLPPLVLRVHLPPDYPsHSPPIFEL-SAPWLSGEERSELCAEL 76

                        90       100
                ....*....|....*....|....
gi 20531755  88 ALQAEENLGMVMIFTLVTAVQEKL 111
Cdd:cd23821  77 DEIWEENAGEPVLFQWVEWLREYL 100
RWD_RWDD3 cd23819
RWD domain of RWD domain-containing protein 3 (RWDD3) and related proteins; RWDD3, also called ...
 11-112 4.52e-13

RWD domain of RWD domain-containing protein 3 (RWDD3) and related proteins; RWDD3, also called RWD domain-containing sumoylation enhancer (RSUME), acts as an enhancer of SUMO conjugation and has no effect on ubiquitination. It increases protein sumoylation (a dynamic ubiquitin-like post translational modification) of several proteins including HIF1alpha and I-kappa-B, through direct interaction with UBC9. Its RWD domain is required for the sumoylation enhancement activity.


Pssm-ID: 467655  Cd Length: 106  Bit Score: 63.50  E-value: 4.52e-13
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 20531755  11 ELEALESIY--PDSFTVLSESPPSFTITVT---SEAGENDETVqTTLKFTYSEKYPDETPLYEIfSQENLEDNDVSDILK 85
Cdd:cd23819   2 ELSVLQAIFcgPGEFEVLSSSETSDGVSFKiqiSVEGFDEDIV-LKLTFHLSPNYPSSLPDISV-SSEQLTRAQCNDLQD 79

                        90       100
                ....*....|....*....|....*..
gi 20531755  86 LLALQAEENLGMVMIFTLVTAVQEKLN 112
Cdd:cd23819  80 SLLEYANSLLGEPMVLELVLWLQENLL 106
RWD_YLR419W-like cd23827
RWD domain of Saccharomyces cerevisiae putative ATP-dependent RNA helicase YLR419W and related ...
 9-115 4.07e-12

RWD domain of Saccharomyces cerevisiae putative ATP-dependent RNA helicase YLR419W and related proteins; YLR419W (EC 3.6.4.13) may act as an ATP-binding RNA helicase. The RWD domain may mediate protein-protein interactions.


Pssm-ID: 467662  Cd Length: 104  Bit Score: 60.72  E-value: 4.07e-12
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 20531755   9 RNELEALESIYPDSFTVLSESppSFTITVTSEAGENDetvQTTLKFTYSEKYPDETPLYEIFSQENLEDNDVSDILKLLA 88
Cdd:cd23827   2 DEEIEALEAIYGEKFEVISDD--SCEITLNSPTKTKP---SLKLKFYKSSSYPNSLPGIFISSSDKLPAYIKLAIIRQLL 76

                        90       100
                ....*....|....*....|....*...
gi 20531755  89 LQAEEN-LGMVMIFTLVTAVQEKLNEIV 115
Cdd:cd23827  77 QYARDNlLGDPMIFSIVEWLEENIEEII 104
RWD-RWDD4 cd23817
RWD domain of RWD domain-containing protein 4 (RWDD4) and related proteins; RWDD4, also called ...
 11-113 2.11e-09

RWD domain of RWD domain-containing protein 4 (RWDD4) and related proteins; RWDD4, also called protein FAM28A, is a target of the tumor suppressor MicroRNA (MiR)-506 in bladder cancer cells. Downregulation of RWDD4 suppresses bladder cancer cell proliferation, migration and invasion. RWDD4 has also been identified as a modifier of metastasis in human prostate cancer.


Pssm-ID: 467653  Cd Length: 104  Bit Score: 53.29  E-value: 2.11e-09
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 20531755  11 ELEALESIYP--DSFTVLSESPPSFTItvtseaGENDETVQTTLKFTYSEKYPDETPLY--EIFSQENLEDNDVSDILKL 86
Cdd:cd23817   3 ELEVLLSIYEgdENFKQISDTTFQYKY------GEDGDPKSFLLEISWPENYPEEPPIInlDAFYNKHISSSVKEKIVSK 76

                        90       100
                ....*....|....*....|....*..
gi 20531755  87 LALQAEENLGMVMIFTLVTAVQEKLNE 113
Cdd:cd23817  77 LNEEAEQNLGSAMTYTLFEWAKENAEE 103
RWD_ScGIR2-like cd23824
RWD domain of Saccharomyces cerevisiae genetically interacts with ribosomal genes protein 2 ...
 4-82 3.14e-09

RWD domain of Saccharomyces cerevisiae genetically interacts with ribosomal genes protein 2 (GIR2) and related proteins; GIR2, also called DRG family-regulatory protein 2, acts as a negative regulator of GCN2 kinase activity by interacting with GCN1 and disrupting the GCN1-GCN2 interaction in amino acid-starved cells. It also interacts with RBG1. The RBG1-GIR2 complex is responsible for efficient cell growth under conditions of amino acid depletion and binds the activator GCN1. Critical mutations in the RWD domain of GIR2 abolishes its interaction with GCN1. GIR2 may interact with ribosomes through GCN1, which binds directly to ribosomes.


Pssm-ID: 467660  Cd Length: 159  Bit Score: 54.25  E-value: 3.14e-09
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 20531755   4 YGEEQRNELEALESIYPDSFTVLSESPPS--FTITVTSEAGENDETVQTTLK-------FTYSEKYPDETPLYEIFSQE- 73
Cdd:cd23824   1 YKEEQEQELEVLESIYPDELTVLNDEYPNiqFEVDLKLELDPLDDTADSLSKehtlvvdFQLPENYPDEAPIISIEPQEv 80


                ....*....
gi 20531755  74 NLEDNDVSD 82
Cdd:cd23824  81 SLKDEDEEE 89
RWD_RNF14 cd23820
RWD domain of RING finger protein 14 (RNF14) and related proteins; RNF14, also called androgen ...
 8-111 1.41e-08

RWD domain of RING finger protein 14 (RNF14) and related proteins; RNF14, also called androgen receptor (AR)-associated protein 54 (ARA54), HFB30, or Triad2 protein, is an RBR-type E3 ubiquitin-protein ligase (EC 2.3.2.31) that is highly expressed in the testis and interacts with class III E2s (UBE2E2, UbcH6, and UBE2E3). Its differential localization may play an important role in testicular development and spermatogenesis in humans. RNF14 functions as a transcriptional regulator of mitochondrial and immune function in muscles. It is a ligand-dependent AR co-activator that enhances AR-dependent transcriptional activation. It also may participate in enhancing cell cycle progression and cell proliferation via induction of cyclin D1. Moreover, RNF14 is crucial for colon cancer cell survival. It acts as a new enhancer of Wnt-dependent transcriptional outputs that act at the level of the T-cell factor/lymphoid enhancer factor (TCF/LEF)-beta-catenin complex.


Pssm-ID: 467656 [Multi-domain]  Cd Length: 125  Bit Score: 51.59  E-value: 1.41e-08
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 20531755   8 QRNELEALESIYPDSFTV------------LSESPPSFTITVTSEAGENDETVQT--------TLKFTYSEKYPDETPLy 67
Cdd:cd23820   1 QEDELEALEAIYPDDLVVdsdsssgrgsleIPVELEPPLSVVLSSDGSDEGERTLkvshlppiTLRFSLPPGYPSTSPP- 79

                        90       100       110       120
                ....*....|....*....|....*....|....*....|....*.
gi 20531755  68 eIFSQEN--LEDNDVSDILKLLALQAEENLGMVMIFTLVTAVQEKL 111
Cdd:cd23820  80 -EFTLECswLSPEQLSALCERLDELWEENGGDVVLFSWIDFLQDEA 124
RWD_ScYIH1-like cd23822
RWD domain of Saccharomyces cerevisiae protein YIH1 and related proteins; YIH1, also called ...
 9-111 3.16e-04

RWD domain of Saccharomyces cerevisiae protein YIH1 and related proteins; YIH1, also called protein IMPACT homolog, is an actin-binding protein that acts as a translational regulator which ensures constant high levels of translation under amino acid starvation. It plays a role as a negative regulator of GCN2 kinase activity. It impairs GCN1-mediated GCN2 activation, and hence GCN2-mediated eIF-2-alpha phosphorylation in amino acid-starved cells and subsequent down-regulation of protein synthesis. In normal conditions, it resides in an actin complex and has no activity.


Pssm-ID: 467658  Cd Length: 97  Bit Score: 38.78  E-value: 3.16e-04
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 20531755   9 RNELEALESIYPDSFTVLSESppSFTITVTseageNDETVQTTLKFtySEKYPDETP--LYEIFSQENLEDNDVSDILKL 86
Cdd:cd23822   2 ADEIEAINAIYPDCLVRLSPS--IYTLKIP-----DHEDVSIQLSF--PSDYPDEPPhvLGVSSSGARGDGKYLKDLLED 72

                        90       100
                ....*....|....*....|....*
gi 20531755  87 LaLQAEENLGMVMIFTLVTAVQEKL 111
Cdd:cd23822  73 I-LASVFVPGEVCLFDFIEELREVL 96
DFRP_C pfam16543
DRG Family Regulatory Proteins, Tma46; DFRP_C is a family of eukaryotic translation ...
 144-197 3.86e-04

DRG Family Regulatory Proteins, Tma46; DFRP_C is a family of eukaryotic translation machinery-associated protein 46 proteins that are the binding partner for the highly conserved Developmentally Regulated GTP-binding (DRG) GTPases. Thus this family is referred to as DRG Family Regulatory Proteins (DFRP). Binding of this DFRP modulates the function of the GTPase.


Pssm-ID: 465167  Cd Length: 89  Bit Score: 38.34  E-value: 3.86e-04
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....
gi 20531755   144 TPVTIENFLSWKAKfdaELLEIKKKRMKEEEQAGKNKLSGKQLFETDHNLDTSD 197
Cdd:pfam16543  20 TPVTPETFAKWKKK---RIDKKEAEEEAERKKKKADGLSGRELFEFNPDLFEDD 70
COG5252 COG5252
Uncharacterized conserved protein, contains CCCH-type Zn-finger protein [General function ...
 144-235 9.35e-04

Uncharacterized conserved protein, contains CCCH-type Zn-finger protein [General function prediction only];


Pssm-ID: 227577 [Multi-domain]  Cd Length: 299  Bit Score: 39.69  E-value: 9.35e-04
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 20531755 144 TPVTIENFLSWK-AKFDAELLEIKKKRMKEEEQAGKNKLSGKQLFETDHNLdtsdiqFLEDAGNNVEVDESLFQemDDLE 222
Cdd:COG5252 212 TPLTEENFKEWKdGRRLRILKQKEEKESARKVKGRATGTKGVELFETRRDL------FKDDVEAGEEWDYTERC--YSES 283

                        90
                ....*....|...
gi 20531755 223 LEDGEDDPDYNPV 235
Cdd:COG5252 284 EDEEGHDKATAPI 296
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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