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Conserved domains on  [gi|316989501|gb|ADU76812|]
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high affinity nitrate transporter, partial [uncultured diatom]

Protein Classification

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
PLN00028 super family cl30556
nitrate transmembrane transporter; Provisional
 10-163 1.26e-65

nitrate transmembrane transporter; Provisional


The actual alignment was detected with superfamily member PLN00028:

Pssm-ID: 177665 [Multi-domain]  Cd Length: 476  Bit Score: 207.47  E-value: 1.26e-65
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 316989501  10 VTQIVMgSALFPLFKT-GMSAEAAWRTVAIVPAVFGFATGFTIIRIGDDCPKGNYRDMKEKGIMPEVSATSSFRDGALNI 88
Cdd:PLN00028 174 VTQLLM-PLVFPLIKDaGAPSFTAWRIAFFVPGLLHIIMGILVLTLGQDLPDGNYRELKKSGTMPKDSFSKVLRYGVTNY 252

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 316989501  89 NTWLLFIQYGCCFGVELTMNNASATYFKETFLLSTESAAAIASIFGWMNLFARGLGGFTSDKFNASMGMRGRLCT 163
Cdd:PLN00028 253 RTWILALTYGYSFGVELTMDNIIAEYFYDRFGLSLETAGAIAASFGLMNLFARPAGGYLSDVAARRFGMRGRLWA 327
 
Name Accession Description Interval E-value
PLN00028 PLN00028
nitrate transmembrane transporter; Provisional
 10-163 1.26e-65

nitrate transmembrane transporter; Provisional


Pssm-ID: 177665 [Multi-domain]  Cd Length: 476  Bit Score: 207.47  E-value: 1.26e-65
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 316989501  10 VTQIVMgSALFPLFKT-GMSAEAAWRTVAIVPAVFGFATGFTIIRIGDDCPKGNYRDMKEKGIMPEVSATSSFRDGALNI 88
Cdd:PLN00028 174 VTQLLM-PLVFPLIKDaGAPSFTAWRIAFFVPGLLHIIMGILVLTLGQDLPDGNYRELKKSGTMPKDSFSKVLRYGVTNY 252

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 316989501  89 NTWLLFIQYGCCFGVELTMNNASATYFKETFLLSTESAAAIASIFGWMNLFARGLGGFTSDKFNASMGMRGRLCT 163
Cdd:PLN00028 253 RTWILALTYGYSFGVELTMDNIIAEYFYDRFGLSLETAGAIAASFGLMNLFARPAGGYLSDVAARRFGMRGRLWA 327
2A0108 TIGR00886
nitrite extrusion protein (nitrite facilitator); [Transport and binding proteins, Anions]
 10-154 5.89e-23

nitrite extrusion protein (nitrite facilitator); [Transport and binding proteins, Anions]


Pssm-ID: 273322 [Multi-domain]  Cd Length: 354  Bit Score: 92.75  E-value: 5.89e-23
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 316989501   10 VTQIVMGS-ALFPLFKTGMSAEAAWRT-VAIVPAVFGFATGFTIIRIGDDCPKGNyrdmkekgIMPEVSATSSFRDGALN 87
Cdd:TIGR00886 141 VAQFVMPPiIGSLIFGGGQPAHLAWGWaFVIVPAGILLLPALLIFFVGADTPPGK--------PATGKLSFKEALKVFGN 212

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 316989501   88 INTWLLFIQYGCCFGVELTMNNASATYFKETFLLSTESAAAIASIFGWMNLFARGLGGFTSDKFNAS 154
Cdd:TIGR00886 213 RHTWILALLYSVTFGSFLGVSSIFAMLFKDQFGLSKVQAGAYASLGGFLGSLARPAGGAISDRLGGA 279
MFS_NRT2_like cd17341
Plant Nitrate transporter NRT2 family and Bacterial Nitrate/Nitrite transporters of the Major ...
 32-161 2.57e-16

Plant Nitrate transporter NRT2 family and Bacterial Nitrate/Nitrite transporters of the Major Facilitator Superfamily; This family is composed of plant NRT2 family high-affinity nitrate transporters as well as nitrate and nitrite transporters from bacteria including Bacillus subtilis nitrate transporter NasA and nitrite extrusion protein NarK, Staphylococcus aureus NarT, Synechococcus sp. nitrate permease NapA, Mycobacterium tuberculosis NarK2 and nitrite extrusion protein NarU. NRT2 family proteins are involved in the uptake of nitrate by plant roots from the soil through the high-affinity transport system (HATS). There are seven Arabidopsis thaliana NRT2 proteins, called AtNRT2:1 to AtNRT2:7. The NRT2-like family belongs to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 340899 [Multi-domain]  Cd Length: 384  Bit Score: 74.60  E-value: 2.57e-16
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 316989501  32 AWRTVAIVPAVFGFATGFTIIRIGDDCPKGNYrdmkekgimPEVSATSSFRDGALNINTWLLFIQYGCCFGVELTMNNAS 111
Cdd:cd17341  158 GWRNAFFVPGILLAIAAVLVLLFGDDLPTGKA---------SALEFLKVQLAVLKNYRTWILSLLYGVTFGSFVGFSNVL 228

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|
gi 316989501 112 ATYFKETFLLSTESAAAIASIFGWMNLFARGLGGFTSDKFnasmGMRGRL 161
Cdd:cd17341  229 PTYFKTRFGLPLVTAGILAALFPLAGALARPAGGWLSDRF----GGRRRL 274
NarK COG2223
Nitrate/nitrite transporter NarK [Inorganic ion transport and metabolism];
15-151 8.31e-14

Nitrate/nitrite transporter NarK [Inorganic ion transport and metabolism];


Pssm-ID: 441825 [Multi-domain]  Cd Length: 392  Bit Score: 67.60  E-value: 8.31e-14
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 316989501  15 MGSALFPLFKTGMSAEAAWRTVAIVPAVFGFATGFTIIRIGDDCPKGNYRDMKekgimpevSATSSFRDGALNINTWLLF 94
Cdd:COG2223  140 LGAAVAAFLAPLLIAAFGWRNAFLILGILLLVVAVLAWLFLRDPPAGAAAAAK--------ASLRDQLEALRDPRFWLLS 211

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....*..
gi 316989501  95 IQYGCCFGVELTMNNASATYFKETFLLSTESAAAIASIFGWMNLFARGLGGFTSDKF 151
Cdd:COG2223  212 LLYFGTFGSFIGFSSWLPPYLVDQFGLSAATAGLLAALFALLGALGRPLGGWLSDRI 268
 
Name Accession Description Interval E-value
PLN00028 PLN00028
nitrate transmembrane transporter; Provisional
 10-163 1.26e-65

nitrate transmembrane transporter; Provisional


Pssm-ID: 177665 [Multi-domain]  Cd Length: 476  Bit Score: 207.47  E-value: 1.26e-65
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 316989501  10 VTQIVMgSALFPLFKT-GMSAEAAWRTVAIVPAVFGFATGFTIIRIGDDCPKGNYRDMKEKGIMPEVSATSSFRDGALNI 88
Cdd:PLN00028 174 VTQLLM-PLVFPLIKDaGAPSFTAWRIAFFVPGLLHIIMGILVLTLGQDLPDGNYRELKKSGTMPKDSFSKVLRYGVTNY 252

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 316989501  89 NTWLLFIQYGCCFGVELTMNNASATYFKETFLLSTESAAAIASIFGWMNLFARGLGGFTSDKFNASMGMRGRLCT 163
Cdd:PLN00028 253 RTWILALTYGYSFGVELTMDNIIAEYFYDRFGLSLETAGAIAASFGLMNLFARPAGGYLSDVAARRFGMRGRLWA 327
2A0108 TIGR00886
nitrite extrusion protein (nitrite facilitator); [Transport and binding proteins, Anions]
 10-154 5.89e-23

nitrite extrusion protein (nitrite facilitator); [Transport and binding proteins, Anions]


Pssm-ID: 273322 [Multi-domain]  Cd Length: 354  Bit Score: 92.75  E-value: 5.89e-23
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 316989501   10 VTQIVMGS-ALFPLFKTGMSAEAAWRT-VAIVPAVFGFATGFTIIRIGDDCPKGNyrdmkekgIMPEVSATSSFRDGALN 87
Cdd:TIGR00886 141 VAQFVMPPiIGSLIFGGGQPAHLAWGWaFVIVPAGILLLPALLIFFVGADTPPGK--------PATGKLSFKEALKVFGN 212

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 316989501   88 INTWLLFIQYGCCFGVELTMNNASATYFKETFLLSTESAAAIASIFGWMNLFARGLGGFTSDKFNAS 154
Cdd:TIGR00886 213 RHTWILALLYSVTFGSFLGVSSIFAMLFKDQFGLSKVQAGAYASLGGFLGSLARPAGGAISDRLGGA 279
MFS_NRT2_like cd17341
Plant Nitrate transporter NRT2 family and Bacterial Nitrate/Nitrite transporters of the Major ...
 32-161 2.57e-16

Plant Nitrate transporter NRT2 family and Bacterial Nitrate/Nitrite transporters of the Major Facilitator Superfamily; This family is composed of plant NRT2 family high-affinity nitrate transporters as well as nitrate and nitrite transporters from bacteria including Bacillus subtilis nitrate transporter NasA and nitrite extrusion protein NarK, Staphylococcus aureus NarT, Synechococcus sp. nitrate permease NapA, Mycobacterium tuberculosis NarK2 and nitrite extrusion protein NarU. NRT2 family proteins are involved in the uptake of nitrate by plant roots from the soil through the high-affinity transport system (HATS). There are seven Arabidopsis thaliana NRT2 proteins, called AtNRT2:1 to AtNRT2:7. The NRT2-like family belongs to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 340899 [Multi-domain]  Cd Length: 384  Bit Score: 74.60  E-value: 2.57e-16
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 316989501  32 AWRTVAIVPAVFGFATGFTIIRIGDDCPKGNYrdmkekgimPEVSATSSFRDGALNINTWLLFIQYGCCFGVELTMNNAS 111
Cdd:cd17341  158 GWRNAFFVPGILLAIAAVLVLLFGDDLPTGKA---------SALEFLKVQLAVLKNYRTWILSLLYGVTFGSFVGFSNVL 228

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|
gi 316989501 112 ATYFKETFLLSTESAAAIASIFGWMNLFARGLGGFTSDKFnasmGMRGRL 161
Cdd:cd17341  229 PTYFKTRFGLPLVTAGILAALFPLAGALARPAGGWLSDRF----GGRRRL 274
NarK COG2223
Nitrate/nitrite transporter NarK [Inorganic ion transport and metabolism];
15-151 8.31e-14

Nitrate/nitrite transporter NarK [Inorganic ion transport and metabolism];


Pssm-ID: 441825 [Multi-domain]  Cd Length: 392  Bit Score: 67.60  E-value: 8.31e-14
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 316989501  15 MGSALFPLFKTGMSAEAAWRTVAIVPAVFGFATGFTIIRIGDDCPKGNYRDMKekgimpevSATSSFRDGALNINTWLLF 94
Cdd:COG2223  140 LGAAVAAFLAPLLIAAFGWRNAFLILGILLLVVAVLAWLFLRDPPAGAAAAAK--------ASLRDQLEALRDPRFWLLS 211

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....*..
gi 316989501  95 IQYGCCFGVELTMNNASATYFKETFLLSTESAAAIASIFGWMNLFARGLGGFTSDKF 151
Cdd:COG2223  212 LLYFGTFGSFIGFSSWLPPYLVDQFGLSAATAGLLAALFALLGALGRPLGGWLSDRI 268
FucP COG0738
Fucose permease [Carbohydrate transport and metabolism];
15-153 1.14e-03

Fucose permease [Carbohydrate transport and metabolism];


Pssm-ID: 440501 [Multi-domain]  Cd Length: 391  Bit Score: 38.30  E-value: 1.14e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 316989501  15 MGSALFPLFKTGM---SAEAAWRTVAIVPAVFGFATGFTIIRIgddcPKGNYRDMKEKGIMPEVSATSSFRDGALnintW 91
Cdd:COG0738  142 LGALLGPLLGGLLillGLSLSWHLPYLILAVLLLLLALLFLRS----KLPEIEEEEEEAAGSAASLKSLLKNPRL----L 213

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 316989501  92 LLFIQYGCCFGVELTMNNASATYFKETFLLSTESAAAIASIFGWMNLFARGLGGFTSDKFNA 153
Cdd:COG0738  214 LGGLAIFLYVGAEGAIGDWLPLYLKDVLGLSEATAALGLSLFWGGMTIGRFLGGFLLKRFGP 275
UhpC COG2271
Sugar phosphate permease [Carbohydrate transport and metabolism];
88-151 1.45e-03

Sugar phosphate permease [Carbohydrate transport and metabolism];


Pssm-ID: 441872 [Multi-domain]  Cd Length: 363  Bit Score: 37.93  E-value: 1.45e-03
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 316989501  88 INTWLLFIQYGCCFGVELTMNNASATYFKETFLLSTESAAAIASIFGWMNLFARGLGGFTSDKF 151
Cdd:COG2271  180 LRFWLLALAYFLVYFALYGFLTWLPTYLVEVRGLSLAQAGLLLSLPFLAGIVGSLLGGWLSDRL 243
MFS cd06174
Major Facilitator Superfamily; The Major Facilitator Superfamily (MFS) is a large and diverse ...
 11-151 4.79e-03

Major Facilitator Superfamily; The Major Facilitator Superfamily (MFS) is a large and diverse group of secondary transporters that includes uniporters, symporters, and antiporters. MFS proteins facilitate the transport across cytoplasmic or internal membranes of a variety of substrates including ions, sugar phosphates, drugs, neurotransmitters, nucleosides, amino acids, and peptides. They do so using the electrochemical potential of the transported substrates. Uniporters transport a single substrate, while symporters and antiporters transport two substrates in the same or in opposite directions, respectively, across membranes. MFS proteins are typically 400 to 600 amino acids in length, and the majority contain 12 transmembrane alpha helices (TMs) connected by hydrophilic loops. The N- and C-terminal halves of these proteins display weak similarity and may be the result of a gene duplication/fusion event. Based on kinetic studies and the structures of a few bacterial superfamily members, GlpT (glycerol-3-phosphate transporter), LacY (lactose permease), and EmrD (multidrug transporter), MFS proteins are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement. Bacterial members function primarily for nutrient uptake, and as drug-efflux pumps to confer antibiotic resistance. Some MFS proteins have medical significance in humans such as the glucose transporter Glut4, which is impaired in type II diabetes, and glucose-6-phosphate transporter (G6PT), which causes glycogen storage disease when mutated.


Pssm-ID: 349949 [Multi-domain]  Cd Length: 378  Bit Score: 36.25  E-value: 4.79e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 316989501  11 TQIVMGSALFPLF--KTGMSAEAAWRTVAIVPAVFGFATGFTIIRIGDDCPKGNYRDMKEKgimPEVSATSSFRDGALNI 88
Cdd:cd06174  127 AFGSVGGILGPLLggILASSLGFGWRAVFLIAAALALLAAILLLLVVPDPPESARAKNEEA---SSKSVLKLLKRVLKNP 203

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 316989501  89 NTWLLFIQYGCCFGVELTMNNASATYFKETFLLSTESAAAIASIFGWMNLFARGLGGFTSDKF 151
Cdd:cd06174  204 GLWLLLLAIFLVNLAYYSFSTLLPLFLLDLGGLSVAVAGLLLSLFGLAGALGSLLLGLLSDRL 266
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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