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Conserved domains on  [gi|388429193|gb|AFK30405|]
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delta-endotoxin [Bacillus thuringiensis]

Protein Classification

glycoside hydrolase family 16 protein; glycoside hydrolase family 43 protein( domain architecture ID 10265820)

glycoside hydrolase family 16 protein similar to endo-beta-1,3-glucanase (also called laminarinase), which hydrolyzes 1,3-beta-D-glucosidic linkages in 1,3-beta-D-glucans such as laminarins, curdlans, paramylons, lichenans, and pachymans| glycoside hydrolase family 43 (GH43) protein such as alpha-L-arabinofuranosidase and beta-D-xylosidase, which hydrolyzes monosaccharide residues from the non-reducing termini of substrates

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
Endotoxin_mid pfam09131
Bacillus thuringiensis delta-Endotoxin, middle domain; Members of this family adopt a ...
 267-472 7.38e-143

Bacillus thuringiensis delta-Endotoxin, middle domain; Members of this family adopt a structure consisting of three four-stranded beta-sheets, each with a Greek key fold, with internal pseudo threefold symmetry. Thus they act as a receptor binding beta-prism, binding to insect-specific receptors of gut epithelial cells.


:

Pssm-ID: 117687  Cd Length: 206  Bit Score: 413.28  E-value: 7.38e-143
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 388429193  267 LMVSSGANLYASGSGPQQTQSFTAQNWPFLYSLFQVNSNYILSGISGTRLSITFPNIGGLPGSTTTHSLNSARVNYSGGV 346
Cdd:pfam09131   1 LLVSSGANLYASGSGPQQTQSFTAQNWPFLYSLFQVNSNYVLSGISGARLSITFPNIGGLPGSTTTQSLNSARVNYSGGV 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 388429193  347 SSGLIGATNLNHNFNCSTVLPPLSTPFVRSWLDSGTDREGVATSTNWQTESFQTTLSLRCGAFSARGNSNYFPDYFIRNI 426
Cdd:pfam09131  81 SSGLIGATNLNQNFNCSTVLPPLSTPFVRSWLDSGTDREGVATSTNWQTESFETTLSLRCGAFSARGNSNYFPDYFIRNI 160

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|....*.
gi 388429193  427 SGVPLVIRNEDLTRPLHYNQIRNIESPSGTPGGARAYLVSVHNRKN 472
Cdd:pfam09131 161 SGVPLVIRNEDLTRPLYYNEIRNIESPSGTPGGARAYLVSVHNRKN 206
Endotoxin_N super family cl04339
delta endotoxin, N-terminal domain; This family contains insecticidal toxins produced by ...
 80-262 9.10e-27

delta endotoxin, N-terminal domain; This family contains insecticidal toxins produced by Bacillus species of bacteria. During spore formation the bacteria produce crystals of this protein. When an insect ingests these proteins they are activated by proteolytic cleavage. The N terminus is cleaved in all of the proteins and a C terminal extension is cleaved in some members. Once activated the endotoxin binds to the gut epithelium and causes cell lysis leading to death. This activated region of the delta endotoxin is composed of three structural domains. The N-terminal helical domain is involved in membrane insertion and pore formation. The second and third domains are involved in receptor binding.


The actual alignment was detected with superfamily member pfam03945:

Pssm-ID: 397850  Cd Length: 218  Bit Score: 108.39  E-value: 9.10e-27
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 388429193   80 IIFPSGSTNLMQDILRETEQFL-NQRLNTDTLARVNAELIGLQANIREFNQQVDNF-LNPTQNPvplsITSSVNTMQQLF 157
Cdd:pfam03945  22 LIETDKSTPELFNSYRQMIQDLiNNSLTQYDDNKLKALLQGCSLSLKDFRTHVKNLkADPKNAT----LKSTVNTIYLNL 97

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 388429193  158 LNRLPQFQI-----QGYQLLLLPLFAQAANMHLSFIRDVILNADEWGISAATLRTYRDYLRNYTRDYSNYCINTYQTAFR 232
Cdd:pfam03945  98 ENDIPQKYLkdcrkEGYEAYELPLFVLMATMHLGLLKEGISNGKDWGISDSDVKIFIDKFNKWIVKYAEYCYTAYVKGLA 177

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|
gi 388429193  233 GLN----------TRLHDMLEFRTYMFLNVFEYVSIWSLF 262
Cdd:pfam03945 178 KIQkesanikdpvKQWNDLNDFRNFMILYVFDFVNLWWAF 217
Endotoxin_C pfam03944
delta endotoxin; This family contains insecticidal toxins produced by Bacillus species of ...
 494-628 1.27e-13

delta endotoxin; This family contains insecticidal toxins produced by Bacillus species of bacteria. During spore formation the bacteria produce crystals of this protein. When an insect ingests these proteins they are activated by proteolytic cleavage. The N terminus is cleaved in all of the proteins and a C terminal extension is cleaved in some members. Once activated the endotoxin binds to the gut epithelium and causes cell lysis leading to death. This activated region of the delta endotoxin is composed of three structural domains. The N-terminal helical domain is involved in membrane insertion and pore formation. The second and third domains are involved in receptor binding.


:

Pssm-ID: 397849  Cd Length: 142  Bit Score: 68.48  E-value: 1.27e-13
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 388429193  494 FTISPIHATQVNNQTRTFISEKFGNQGDSLRFEQSNTT-ARYTLRGNGNS---YNLYLRVSSIGNSTIRVTINGRVYTVS 569
Cdd:pfam03944   1 ITQIPAVKAYNLGSGASVVKGPGFTGGDLLKLRNSGGLlGRIRVTVNAPLsqrYRIRIRYASTTDSQLSVNIGGSGGNQI 80

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 388429193  570 NVNTTTNN-DGVNDNGARFSDINIG-NIVASDNTNVTLDINVT-LNSGTPFDLMNIMFVPTN 628
Cdd:pfam03944  81 NFPSTMSGgDNLTLNYGSFQYVEFStPFTFSENPEITLTLSITnFSSNGELYIDRIEFIPVN 142
 
Name Accession Description Interval E-value
Endotoxin_mid pfam09131
Bacillus thuringiensis delta-Endotoxin, middle domain; Members of this family adopt a ...
 267-472 7.38e-143

Bacillus thuringiensis delta-Endotoxin, middle domain; Members of this family adopt a structure consisting of three four-stranded beta-sheets, each with a Greek key fold, with internal pseudo threefold symmetry. Thus they act as a receptor binding beta-prism, binding to insect-specific receptors of gut epithelial cells.


Pssm-ID: 117687  Cd Length: 206  Bit Score: 413.28  E-value: 7.38e-143
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 388429193  267 LMVSSGANLYASGSGPQQTQSFTAQNWPFLYSLFQVNSNYILSGISGTRLSITFPNIGGLPGSTTTHSLNSARVNYSGGV 346
Cdd:pfam09131   1 LLVSSGANLYASGSGPQQTQSFTAQNWPFLYSLFQVNSNYVLSGISGARLSITFPNIGGLPGSTTTQSLNSARVNYSGGV 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 388429193  347 SSGLIGATNLNHNFNCSTVLPPLSTPFVRSWLDSGTDREGVATSTNWQTESFQTTLSLRCGAFSARGNSNYFPDYFIRNI 426
Cdd:pfam09131  81 SSGLIGATNLNQNFNCSTVLPPLSTPFVRSWLDSGTDREGVATSTNWQTESFETTLSLRCGAFSARGNSNYFPDYFIRNI 160

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|....*.
gi 388429193  427 SGVPLVIRNEDLTRPLHYNQIRNIESPSGTPGGARAYLVSVHNRKN 472
Cdd:pfam09131 161 SGVPLVIRNEDLTRPLYYNEIRNIESPSGTPGGARAYLVSVHNRKN 206
Endotoxin_N pfam03945
delta endotoxin, N-terminal domain; This family contains insecticidal toxins produced by ...
 80-262 9.10e-27

delta endotoxin, N-terminal domain; This family contains insecticidal toxins produced by Bacillus species of bacteria. During spore formation the bacteria produce crystals of this protein. When an insect ingests these proteins they are activated by proteolytic cleavage. The N terminus is cleaved in all of the proteins and a C terminal extension is cleaved in some members. Once activated the endotoxin binds to the gut epithelium and causes cell lysis leading to death. This activated region of the delta endotoxin is composed of three structural domains. The N-terminal helical domain is involved in membrane insertion and pore formation. The second and third domains are involved in receptor binding.


Pssm-ID: 397850  Cd Length: 218  Bit Score: 108.39  E-value: 9.10e-27
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 388429193   80 IIFPSGSTNLMQDILRETEQFL-NQRLNTDTLARVNAELIGLQANIREFNQQVDNF-LNPTQNPvplsITSSVNTMQQLF 157
Cdd:pfam03945  22 LIETDKSTPELFNSYRQMIQDLiNNSLTQYDDNKLKALLQGCSLSLKDFRTHVKNLkADPKNAT----LKSTVNTIYLNL 97

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 388429193  158 LNRLPQFQI-----QGYQLLLLPLFAQAANMHLSFIRDVILNADEWGISAATLRTYRDYLRNYTRDYSNYCINTYQTAFR 232
Cdd:pfam03945  98 ENDIPQKYLkdcrkEGYEAYELPLFVLMATMHLGLLKEGISNGKDWGISDSDVKIFIDKFNKWIVKYAEYCYTAYVKGLA 177

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|
gi 388429193  233 GLN----------TRLHDMLEFRTYMFLNVFEYVSIWSLF 262
Cdd:pfam03945 178 KIQkesanikdpvKQWNDLNDFRNFMILYVFDFVNLWWAF 217
Endotoxin_C pfam03944
delta endotoxin; This family contains insecticidal toxins produced by Bacillus species of ...
 494-628 1.27e-13

delta endotoxin; This family contains insecticidal toxins produced by Bacillus species of bacteria. During spore formation the bacteria produce crystals of this protein. When an insect ingests these proteins they are activated by proteolytic cleavage. The N terminus is cleaved in all of the proteins and a C terminal extension is cleaved in some members. Once activated the endotoxin binds to the gut epithelium and causes cell lysis leading to death. This activated region of the delta endotoxin is composed of three structural domains. The N-terminal helical domain is involved in membrane insertion and pore formation. The second and third domains are involved in receptor binding.


Pssm-ID: 397849  Cd Length: 142  Bit Score: 68.48  E-value: 1.27e-13
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 388429193  494 FTISPIHATQVNNQTRTFISEKFGNQGDSLRFEQSNTT-ARYTLRGNGNS---YNLYLRVSSIGNSTIRVTINGRVYTVS 569
Cdd:pfam03944   1 ITQIPAVKAYNLGSGASVVKGPGFTGGDLLKLRNSGGLlGRIRVTVNAPLsqrYRIRIRYASTTDSQLSVNIGGSGGNQI 80

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 388429193  570 NVNTTTNN-DGVNDNGARFSDINIG-NIVASDNTNVTLDINVT-LNSGTPFDLMNIMFVPTN 628
Cdd:pfam03944  81 NFPSTMSGgDNLTLNYGSFQYVEFStPFTFSENPEITLTLSITnFSSNGELYIDRIEFIPVN 142
 
Name Accession Description Interval E-value
Endotoxin_mid pfam09131
Bacillus thuringiensis delta-Endotoxin, middle domain; Members of this family adopt a ...
 267-472 7.38e-143

Bacillus thuringiensis delta-Endotoxin, middle domain; Members of this family adopt a structure consisting of three four-stranded beta-sheets, each with a Greek key fold, with internal pseudo threefold symmetry. Thus they act as a receptor binding beta-prism, binding to insect-specific receptors of gut epithelial cells.


Pssm-ID: 117687  Cd Length: 206  Bit Score: 413.28  E-value: 7.38e-143
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 388429193  267 LMVSSGANLYASGSGPQQTQSFTAQNWPFLYSLFQVNSNYILSGISGTRLSITFPNIGGLPGSTTTHSLNSARVNYSGGV 346
Cdd:pfam09131   1 LLVSSGANLYASGSGPQQTQSFTAQNWPFLYSLFQVNSNYVLSGISGARLSITFPNIGGLPGSTTTQSLNSARVNYSGGV 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 388429193  347 SSGLIGATNLNHNFNCSTVLPPLSTPFVRSWLDSGTDREGVATSTNWQTESFQTTLSLRCGAFSARGNSNYFPDYFIRNI 426
Cdd:pfam09131  81 SSGLIGATNLNQNFNCSTVLPPLSTPFVRSWLDSGTDREGVATSTNWQTESFETTLSLRCGAFSARGNSNYFPDYFIRNI 160

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|....*.
gi 388429193  427 SGVPLVIRNEDLTRPLHYNQIRNIESPSGTPGGARAYLVSVHNRKN 472
Cdd:pfam09131 161 SGVPLVIRNEDLTRPLYYNEIRNIESPSGTPGGARAYLVSVHNRKN 206
Endotoxin_N pfam03945
delta endotoxin, N-terminal domain; This family contains insecticidal toxins produced by ...
 80-262 9.10e-27

delta endotoxin, N-terminal domain; This family contains insecticidal toxins produced by Bacillus species of bacteria. During spore formation the bacteria produce crystals of this protein. When an insect ingests these proteins they are activated by proteolytic cleavage. The N terminus is cleaved in all of the proteins and a C terminal extension is cleaved in some members. Once activated the endotoxin binds to the gut epithelium and causes cell lysis leading to death. This activated region of the delta endotoxin is composed of three structural domains. The N-terminal helical domain is involved in membrane insertion and pore formation. The second and third domains are involved in receptor binding.


Pssm-ID: 397850  Cd Length: 218  Bit Score: 108.39  E-value: 9.10e-27
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 388429193   80 IIFPSGSTNLMQDILRETEQFL-NQRLNTDTLARVNAELIGLQANIREFNQQVDNF-LNPTQNPvplsITSSVNTMQQLF 157
Cdd:pfam03945  22 LIETDKSTPELFNSYRQMIQDLiNNSLTQYDDNKLKALLQGCSLSLKDFRTHVKNLkADPKNAT----LKSTVNTIYLNL 97

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 388429193  158 LNRLPQFQI-----QGYQLLLLPLFAQAANMHLSFIRDVILNADEWGISAATLRTYRDYLRNYTRDYSNYCINTYQTAFR 232
Cdd:pfam03945  98 ENDIPQKYLkdcrkEGYEAYELPLFVLMATMHLGLLKEGISNGKDWGISDSDVKIFIDKFNKWIVKYAEYCYTAYVKGLA 177

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|
gi 388429193  233 GLN----------TRLHDMLEFRTYMFLNVFEYVSIWSLF 262
Cdd:pfam03945 178 KIQkesanikdpvKQWNDLNDFRNFMILYVFDFVNLWWAF 217
Endotoxin_C pfam03944
delta endotoxin; This family contains insecticidal toxins produced by Bacillus species of ...
 494-628 1.27e-13

delta endotoxin; This family contains insecticidal toxins produced by Bacillus species of bacteria. During spore formation the bacteria produce crystals of this protein. When an insect ingests these proteins they are activated by proteolytic cleavage. The N terminus is cleaved in all of the proteins and a C terminal extension is cleaved in some members. Once activated the endotoxin binds to the gut epithelium and causes cell lysis leading to death. This activated region of the delta endotoxin is composed of three structural domains. The N-terminal helical domain is involved in membrane insertion and pore formation. The second and third domains are involved in receptor binding.


Pssm-ID: 397849  Cd Length: 142  Bit Score: 68.48  E-value: 1.27e-13
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 388429193  494 FTISPIHATQVNNQTRTFISEKFGNQGDSLRFEQSNTT-ARYTLRGNGNS---YNLYLRVSSIGNSTIRVTINGRVYTVS 569
Cdd:pfam03944   1 ITQIPAVKAYNLGSGASVVKGPGFTGGDLLKLRNSGGLlGRIRVTVNAPLsqrYRIRIRYASTTDSQLSVNIGGSGGNQI 80

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 388429193  570 NVNTTTNN-DGVNDNGARFSDINIG-NIVASDNTNVTLDINVT-LNSGTPFDLMNIMFVPTN 628
Cdd:pfam03944  81 NFPSTMSGgDNLTLNYGSFQYVEFStPFTFSENPEITLTLSITnFSSNGELYIDRIEFIPVN 142
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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