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Conserved domains on  [gi|1039033372|gb|ANM77567|]
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protein TolA [Serratia marcescens]

Protein Classification

energy transducer TonB( domain architecture ID 2264)

energy transducer TonB interacts with outer membrane receptor proteins that carry out high-affinity binding and energy dependent uptake into the periplasmic space of specific substrates such as cobalamin, and various iron compounds (such as iron dicitrate, enterochelin, aerobactin, etc.)

Gene Ontology:  GO:0031992

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
TonB_C super family cl10048
Gram-negative bacterial TonB protein C-terminal; The TonB_C domain is the well-characterized ...
 57-144 6.21e-30

Gram-negative bacterial TonB protein C-terminal; The TonB_C domain is the well-characterized C-terminal region of the TonB receptor molecule. This protein is bound to an inner membrane-bound protein ExbB via a globular domain and has a flexible middle region that is likely to help in positioning the C-terminal domain into the iron-transporter barrel in the outer membrane. TonB_C interacts with the N-terminal TonB box of the outer membrane transporter that binds the Fe3+-siderophore complex. The barrel of the transporter, consisting of 22 beta-sheets and an inside plug, binds the iron complex in the barrel entrance.


The actual alignment was detected with superfamily member pfam06519:

Pssm-ID: 471968 [Multi-domain]  Cd Length: 94  Bit Score: 105.20  E-value: 6.21e-30
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039033372  57 APGKEKEHYIAQLQAAIQSHLKDAEAYSGKRCTLSIKLAPDGMLISIRAEQGEPELCQAAIKAIAN-ARLPKPPTAAVYE 135
Cdd:pfam06519   6 ASGAEVDRYAGQIKQAIQSRFLKDDSFAGKTCRVRIKLAPDGTLISVKVEGGDPALCQAAKAAVAKtAKIPKPPSQDVYE 85


                  ....*....
gi 1039033372 136 AVKSSTLEF 144
Cdd:pfam06519  86 KFKNINLDF 94
 
Name Accession Description Interval E-value
TolA pfam06519
TolA C-terminal; This family consists of several bacterial TolA proteins as well as two ...
 57-144 6.21e-30

TolA C-terminal; This family consists of several bacterial TolA proteins as well as two eukaryotic proteins of unknown function. Tol proteins are involved in the translocation of group A colicins. Colicins are bacterial protein toxins, which are active against Escherichia coli and other related species (See pfam01024). TolA is anchored to the cytoplasmic membrane by a single membrane spanning segment near the N-terminus, leaving most of the protein exposed to the periplasm.


Pssm-ID: 399492 [Multi-domain]  Cd Length: 94  Bit Score: 105.20  E-value: 6.21e-30
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039033372  57 APGKEKEHYIAQLQAAIQSHLKDAEAYSGKRCTLSIKLAPDGMLISIRAEQGEPELCQAAIKAIAN-ARLPKPPTAAVYE 135
Cdd:pfam06519   6 ASGAEVDRYAGQIKQAIQSRFLKDDSFAGKTCRVRIKLAPDGTLISVKVEGGDPALCQAAKAAVAKtAKIPKPPSQDVYE 85


                  ....*....
gi 1039033372 136 AVKSSTLEF 144
Cdd:pfam06519  86 KFKNINLDF 94
tolA PRK09510
cell envelope integrity inner membrane protein TolA; Provisional
 57-147 1.01e-28

cell envelope integrity inner membrane protein TolA; Provisional


Pssm-ID: 236545 [Multi-domain]  Cd Length: 387  Bit Score: 109.13  E-value: 1.01e-28
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039033372  57 APGKEKEHYIAQLQAAIQSHLKDAEAYSGKRCTLSIKLAPDGMLISIRAEQGEPELCQAAIKAIANARLPKPPTAAVYEA 136
Cdd:PRK09510  297 ASGADIDQYAGQIKSAIQSKFYDASSYAGKTCTLRIKLAPDGTLLDIKKEGGDPALCQAALAAAKTAKIPKPPSQEVYEK 376

                          90
                  ....*....|.
gi 1039033372 137 VKSSTLEFRPQ 147
Cdd:PRK09510  377 FKNAPLDFKPQ 387
tolA_full TIGR02794
TolA protein; TolA couples the inner membrane complex of itself with TolQ and TolR to the ...
 51-146 7.40e-20

TolA protein; TolA couples the inner membrane complex of itself with TolQ and TolR to the outer membrane complex of TolB and OprL (also called Pal). Most of the length of the protein consists of low-complexity sequence that may differ in both length and composition from one species to another, complicating efforts to discriminate TolA (the most divergent gene in the tol-pal system) from paralogs such as TonB. Selection of members of the seed alignment and criteria for setting scoring cutoffs are based largely conserved operon struction. //The Tol-Pal complex is required for maintaining outer membrane integrity. Also involved in transport (uptake) of colicins and filamentous DNA, and implicated in pathogenesis. Transport is energized by the proton motive force. TolA is an inner membrane protein that interacts with periplasmic TolB and with outer membrane porins ompC, phoE and lamB. [Transport and binding proteins, Other, Cellular processes, Pathogenesis]


Pssm-ID: 274303 [Multi-domain]  Cd Length: 346  Bit Score: 84.51  E-value: 7.40e-20
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039033372  51 GDPKKPAPGKEKEHYIAQLQAAIQSHLKDAEAYSGKRCTLSIKLAPDGMLISIRAEQGEPELCQAAIKAIANAR-LPKPP 129
Cdd:TIGR02794 250 GGARGAAAGSEVDKYAAIIQQAIQQNLYDDPSFRGKTCRLRIRLAPDGTLLSVTKSSGDPALCQAALAAVAKAAkLPMPP 329

                          90
                  ....*....|....*..
gi 1039033372 130 TAAVYEAVKSSTLEFRP 146
Cdd:TIGR02794 330 SDPVYEEFKNINLTFKP 346
TonB COG0810
Periplasmic protein TonB, links inner and outer membranes [Cell wall/membrane/envelope ...
 87-145 6.09e-04

Periplasmic protein TonB, links inner and outer membranes [Cell wall/membrane/envelope biogenesis];


Pssm-ID: 440572 [Multi-domain]  Cd Length: 70  Bit Score: 36.79  E-value: 6.09e-04
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 1039033372  87 RCTLSIKLAPDGMLISIRAEQ--GEPELCQAAIKAIANARlPKPPTAAvYEAVKSSTLEFR 145
Cdd:COG0810    12 TVTVRFTIDADGRVTDVEVVKssGHPLLDEAALRAVRRWR-FKPAKQN-GKPVRVKTVPIR 70
 
Name Accession Description Interval E-value
TolA pfam06519
TolA C-terminal; This family consists of several bacterial TolA proteins as well as two ...
 57-144 6.21e-30

TolA C-terminal; This family consists of several bacterial TolA proteins as well as two eukaryotic proteins of unknown function. Tol proteins are involved in the translocation of group A colicins. Colicins are bacterial protein toxins, which are active against Escherichia coli and other related species (See pfam01024). TolA is anchored to the cytoplasmic membrane by a single membrane spanning segment near the N-terminus, leaving most of the protein exposed to the periplasm.


Pssm-ID: 399492 [Multi-domain]  Cd Length: 94  Bit Score: 105.20  E-value: 6.21e-30
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039033372  57 APGKEKEHYIAQLQAAIQSHLKDAEAYSGKRCTLSIKLAPDGMLISIRAEQGEPELCQAAIKAIAN-ARLPKPPTAAVYE 135
Cdd:pfam06519   6 ASGAEVDRYAGQIKQAIQSRFLKDDSFAGKTCRVRIKLAPDGTLISVKVEGGDPALCQAAKAAVAKtAKIPKPPSQDVYE 85


                  ....*....
gi 1039033372 136 AVKSSTLEF 144
Cdd:pfam06519  86 KFKNINLDF 94
tolA PRK09510
cell envelope integrity inner membrane protein TolA; Provisional
 57-147 1.01e-28

cell envelope integrity inner membrane protein TolA; Provisional


Pssm-ID: 236545 [Multi-domain]  Cd Length: 387  Bit Score: 109.13  E-value: 1.01e-28
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039033372  57 APGKEKEHYIAQLQAAIQSHLKDAEAYSGKRCTLSIKLAPDGMLISIRAEQGEPELCQAAIKAIANARLPKPPTAAVYEA 136
Cdd:PRK09510  297 ASGADIDQYAGQIKSAIQSKFYDASSYAGKTCTLRIKLAPDGTLLDIKKEGGDPALCQAALAAAKTAKIPKPPSQEVYEK 376

                          90
                  ....*....|.
gi 1039033372 137 VKSSTLEFRPQ 147
Cdd:PRK09510  377 FKNAPLDFKPQ 387
tolA_full TIGR02794
TolA protein; TolA couples the inner membrane complex of itself with TolQ and TolR to the ...
 51-146 7.40e-20

TolA protein; TolA couples the inner membrane complex of itself with TolQ and TolR to the outer membrane complex of TolB and OprL (also called Pal). Most of the length of the protein consists of low-complexity sequence that may differ in both length and composition from one species to another, complicating efforts to discriminate TolA (the most divergent gene in the tol-pal system) from paralogs such as TonB. Selection of members of the seed alignment and criteria for setting scoring cutoffs are based largely conserved operon struction. //The Tol-Pal complex is required for maintaining outer membrane integrity. Also involved in transport (uptake) of colicins and filamentous DNA, and implicated in pathogenesis. Transport is energized by the proton motive force. TolA is an inner membrane protein that interacts with periplasmic TolB and with outer membrane porins ompC, phoE and lamB. [Transport and binding proteins, Other, Cellular processes, Pathogenesis]


Pssm-ID: 274303 [Multi-domain]  Cd Length: 346  Bit Score: 84.51  E-value: 7.40e-20
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039033372  51 GDPKKPAPGKEKEHYIAQLQAAIQSHLKDAEAYSGKRCTLSIKLAPDGMLISIRAEQGEPELCQAAIKAIANAR-LPKPP 129
Cdd:TIGR02794 250 GGARGAAAGSEVDKYAAIIQQAIQQNLYDDPSFRGKTCRLRIRLAPDGTLLSVTKSSGDPALCQAALAAVAKAAkLPMPP 329

                          90
                  ....*....|....*..
gi 1039033372 130 TAAVYEAVKSSTLEFRP 146
Cdd:TIGR02794 330 SDPVYEEFKNINLTFKP 346
TonB_2 pfam13103
TonB C terminal; This family contains TonB members that are not captured by pfam03544.
 65-130 9.33e-08

TonB C terminal; This family contains TonB members that are not captured by pfam03544.


Pssm-ID: 463788 [Multi-domain]  Cd Length: 85  Bit Score: 47.33  E-value: 9.33e-08
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039033372  65 YIAQLQAAIQSHLKDAEAYSGK-RCTLSIKLAPDGMLISIRAEQ--GEPELCQAAIKAIANAR-LPKPPT 130
Cdd:pfam13103   4 YAAKVRAIIRENWAPPSGISGNlEAVVEITIDPDGTIVSVRLVKssGNEAFDKAVLRALEKSSpLPPPPE 73
TonB COG0810
Periplasmic protein TonB, links inner and outer membranes [Cell wall/membrane/envelope ...
 87-145 6.09e-04

Periplasmic protein TonB, links inner and outer membranes [Cell wall/membrane/envelope biogenesis];


Pssm-ID: 440572 [Multi-domain]  Cd Length: 70  Bit Score: 36.79  E-value: 6.09e-04
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 1039033372  87 RCTLSIKLAPDGMLISIRAEQ--GEPELCQAAIKAIANARlPKPPTAAvYEAVKSSTLEFR 145
Cdd:COG0810    12 TVTVRFTIDADGRVTDVEVVKssGHPLLDEAALRAVRRWR-FKPAKQN-GKPVRVKTVPIR 70
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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