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Conserved domains on  [gi|226694210|sp|B6I5D7|]
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RecName: Full=Holo-[acyl-carrier-protein] synthase; Short=Holo-ACP synthase; AltName: Full=4'-phosphopantetheinyl transferase AcpS

Protein Classification

holo-ACP synthase( domain architecture ID 10011191)

holo-[acyl-carrier-protein] synthase transfers the 4'-phosphopantetheine moiety from coenzyme A to a serine of acyl-carrier-protein (ACP)

CATH:  3.90.470.20
EC:  2.7.8.7
Gene Ontology:  GO:0008897|GO:0006633|GO:0000287|GO:0016740
PubMed:  9211277
SCOP:  4003652

Graphical summary

 Zoom to residue level

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List of domain hits

 Name Accession Description Interval E-value
acpS PRK00070
4'-phosphopantetheinyl transferase; Provisional
 2-126 4.56e-63

4'-phosphopantetheinyl transferase; Provisional


:

Pssm-ID: 234610 [Multi-domain]  Cd Length: 126  Bit Score: 188.03  E-value: 4.56e-63
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 226694210   2 AILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHHQPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFN 81
Cdd:PRK00070   1 MIVGIGIDIVEIERIEKALERTGDRFAERVLTPKERAKFKSGKRPAEFLAGRFAAKEAFSKALGTGIGKGVSFRDIEVLN 80

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*
gi 226694210  82 DELGKPRLRLWGEALKLAEKLGVVNMHVTLADERHYACATVIIES 126
Cdd:PRK00070  81 DELGKPIVRLSGEAAERLEKLGGARIHLSISHDGDYAVAFVILES 125
 
Name Accession Description Interval E-value
acpS PRK00070
4'-phosphopantetheinyl transferase; Provisional
 2-126 4.56e-63

4'-phosphopantetheinyl transferase; Provisional


Pssm-ID: 234610 [Multi-domain]  Cd Length: 126  Bit Score: 188.03  E-value: 4.56e-63
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 226694210   2 AILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHHQPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFN 81
Cdd:PRK00070   1 MIVGIGIDIVEIERIEKALERTGDRFAERVLTPKERAKFKSGKRPAEFLAGRFAAKEAFSKALGTGIGKGVSFRDIEVLN 80

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*
gi 226694210  82 DELGKPRLRLWGEALKLAEKLGVVNMHVTLADERHYACATVIIES 126
Cdd:PRK00070  81 DELGKPIVRLSGEAAERLEKLGGARIHLSISHDGDYAVAFVILES 125
AcpS COG0736
Phosphopantetheinyl transferase (holo-ACP synthase) [Lipid transport and metabolism];
5-126 2.27e-57

Phosphopantetheinyl transferase (holo-ACP synthase) [Lipid transport and metabolism];


Pssm-ID: 440499 [Multi-domain]  Cd Length: 122  Bit Score: 173.39  E-value: 2.27e-57
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 226694210   5 GLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHHQPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDEL 84
Cdd:COG0736    1 GIGIDIVEIARIERALERHGERFLERVFTPAERAYCQSRKRPAEFLAGRFAAKEAVSKALGTGIGKGVSWRDIEVLNDPS 80

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|..
gi 226694210  85 GKPRLRLWGEALKLAEKLGVVNMHVTLADERHYACATVIIES 126
Cdd:COG0736   81 GKPTVRLSGRAAELAAELGITRIHLSISHERDYAVAFVILEA 122
pantethn_trn TIGR00556
phosphopantetheine--protein transferase domain; This model models a domain active in ...
 2-126 4.85e-49

phosphopantetheine--protein transferase domain; This model models a domain active in transferring the phophopantetheine prosthetic group to its attachment site on enzymes and carrier proteins. Many members of this family are small proteins that act on the acyl carrier protein involved in fatty acid biosynthesis. Some members are domains of larger proteins involved specialized pathways for the synthesis of unusual molecules including polyketides, atypical fatty acids, and antibiotics. [Protein fate, Protein modification and repair]


Pssm-ID: 273136 [Multi-domain]  Cd Length: 128  Bit Score: 152.98  E-value: 4.85e-49
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 226694210    2 AILGLGTDIVEIARIEAVIARSGeRLARRVLSDNEWAIWKTHHQPVR--FLAKRFAVKEAAAKAFGTGIRNG-LAFNQFE 78
Cdd:TIGR00556   1 DIVGIGIDIVEIKRIAEQIERSG-TFAERFFTPSEIEDYCKLSPKSQteSLAGRWAAKEAFIKALGKGISLGeLLFTDIE 79

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*...
gi 226694210   79 VFNDELGKPRLRLWGEALKLAEKLGVVNMHVTLADERHYACATVIIES 126
Cdd:TIGR00556  80 IVKDLKGAPRVCLIGEAAKDAEKLGVCSVHVSISHDKEYAAAQVILER 127
ACPS pfam01648
4'-phosphopantetheinyl transferase superfamily; Members of this family transfers the 4 ...
 5-112 4.41e-22

4'-phosphopantetheinyl transferase superfamily; Members of this family transfers the 4'-phosphopantetheine (4'-PP) moiety from coenzyme A (CoA) to the invariant serine of pfam00550. This post-translational modification renders holo-ACP capable of acyl group activation via thioesterification of the cysteamine thiol of 4'-PP. This superfamily consists of two subtypes: The ACPS type and the Sfp type. The structure of the Sfp type is known, which shows the active site accommodates a magnesium ion. The most highly conserved regions of the alignment are involved in binding the magnesium ion.


Pssm-ID: 426364 [Multi-domain]  Cd Length: 111  Bit Score: 83.81  E-value: 4.41e-22
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 226694210    5 GLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHHQPVR-FLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDE 83
Cdd:pfam01648   1 GVGIDIEEIARIRRPIERLGERLAERIFTPEERALLASLPAEARrAFARLWTAKEAVFKALGPGLSKLLDFDDIEVLLDP 80

                          90       100
                  ....*....|....*....|....*....
gi 226694210   84 LGKPRLRLWGEALKLAEKLGVVNMHVTLA 112
Cdd:pfam01648  81 DGRPTLRLLGEAADLAWRFEVLAGDYALA 109
 
Name Accession Description Interval E-value
acpS PRK00070
4'-phosphopantetheinyl transferase; Provisional
 2-126 4.56e-63

4'-phosphopantetheinyl transferase; Provisional


Pssm-ID: 234610 [Multi-domain]  Cd Length: 126  Bit Score: 188.03  E-value: 4.56e-63
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 226694210   2 AILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHHQPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFN 81
Cdd:PRK00070   1 MIVGIGIDIVEIERIEKALERTGDRFAERVLTPKERAKFKSGKRPAEFLAGRFAAKEAFSKALGTGIGKGVSFRDIEVLN 80

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*
gi 226694210  82 DELGKPRLRLWGEALKLAEKLGVVNMHVTLADERHYACATVIIES 126
Cdd:PRK00070  81 DELGKPIVRLSGEAAERLEKLGGARIHLSISHDGDYAVAFVILES 125
AcpS COG0736
Phosphopantetheinyl transferase (holo-ACP synthase) [Lipid transport and metabolism];
5-126 2.27e-57

Phosphopantetheinyl transferase (holo-ACP synthase) [Lipid transport and metabolism];


Pssm-ID: 440499 [Multi-domain]  Cd Length: 122  Bit Score: 173.39  E-value: 2.27e-57
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 226694210   5 GLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHHQPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDEL 84
Cdd:COG0736    1 GIGIDIVEIARIERALERHGERFLERVFTPAERAYCQSRKRPAEFLAGRFAAKEAVSKALGTGIGKGVSWRDIEVLNDPS 80

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|..
gi 226694210  85 GKPRLRLWGEALKLAEKLGVVNMHVTLADERHYACATVIIES 126
Cdd:COG0736   81 GKPTVRLSGRAAELAAELGITRIHLSISHERDYAVAFVILEA 122
pantethn_trn TIGR00556
phosphopantetheine--protein transferase domain; This model models a domain active in ...
 2-126 4.85e-49

phosphopantetheine--protein transferase domain; This model models a domain active in transferring the phophopantetheine prosthetic group to its attachment site on enzymes and carrier proteins. Many members of this family are small proteins that act on the acyl carrier protein involved in fatty acid biosynthesis. Some members are domains of larger proteins involved specialized pathways for the synthesis of unusual molecules including polyketides, atypical fatty acids, and antibiotics. [Protein fate, Protein modification and repair]


Pssm-ID: 273136 [Multi-domain]  Cd Length: 128  Bit Score: 152.98  E-value: 4.85e-49
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 226694210    2 AILGLGTDIVEIARIEAVIARSGeRLARRVLSDNEWAIWKTHHQPVR--FLAKRFAVKEAAAKAFGTGIRNG-LAFNQFE 78
Cdd:TIGR00556   1 DIVGIGIDIVEIKRIAEQIERSG-TFAERFFTPSEIEDYCKLSPKSQteSLAGRWAAKEAFIKALGKGISLGeLLFTDIE 79

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*...
gi 226694210   79 VFNDELGKPRLRLWGEALKLAEKLGVVNMHVTLADERHYACATVIIES 126
Cdd:TIGR00556  80 IVKDLKGAPRVCLIGEAAKDAEKLGVCSVHVSISHDKEYAAAQVILER 127
acpS PRK14657
holo-[acyl-carrier-protein] synthase;
3-126 1.70e-35

holo-[acyl-carrier-protein] synthase;


Pssm-ID: 173120 [Multi-domain]  Cd Length: 123  Bit Score: 118.34  E-value: 1.70e-35
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 226694210   3 ILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKThhQPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFND 82
Cdd:PRK14657   2 IVGLGIDITELDRIAKALERFGDRFARRILHPAELAAMPA--APVAFLAGRFAAKEAAVKALGTGFSQGIGPRDIEVGVL 79

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....
gi 226694210  83 ELGKPRLRLWGEALKLAEKLGVVNMHVTLADERHYACATVIIES 126
Cdd:PRK14657  80 PAGAPQLVLHGKALARAEALGATSTHVSLTHGRDTAAAVVVLEG 123
acpS PRK14656
holo-[acyl-carrier-protein] synthase;
3-126 8.28e-29

holo-[acyl-carrier-protein] synthase;


Pssm-ID: 237779 [Multi-domain]  Cd Length: 126  Bit Score: 101.38  E-value: 8.28e-29
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 226694210   3 ILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHHQPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFND 82
Cdd:PRK14656   2 IFGTGVDIVDISRFERFVDEGNVALLERIFTPHEQEYCAGKKHSAQHYALRFAAKEAFLKALGTGLRDGISWHDMEVVND 81

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....
gi 226694210  83 ELGKPRLRLWGEALKLAEKLGVVNMHVTLADERHYACATVIIES 126
Cdd:PRK14656  82 QLGKPELRLYGRALELFAQAGLSKTFLSLSHDGGCAVAMVVLER 125
acpS PRK14660
holo-[acyl-carrier-protein] synthase;
3-125 4.90e-28

holo-[acyl-carrier-protein] synthase;


Pssm-ID: 173123  Cd Length: 125  Bit Score: 99.57  E-value: 4.90e-28
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 226694210   3 ILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHHQPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFND 82
Cdd:PRK14660   2 ILGTGVDIVEVERIARSIERHGDRFLRRIYTPGEIAYCTSKANRAERLAARFAAKEAVMKAIGTGLREGVRWTDFEVCRD 81

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|...
gi 226694210  83 ELGKPRLRLWGEALKLAEKLGVVNMHVTLADERHYACATVIIE 125
Cdd:PRK14660  82 ERGRPTVRLHGRAAEIAAALGATRIHLSLSHTQEYAVAQVILE 124
acpS PRK14659
holo-[acyl-carrier-protein] synthase;
3-124 7.26e-25

holo-[acyl-carrier-protein] synthase;


Pssm-ID: 237780  Cd Length: 122  Bit Score: 91.36  E-value: 7.26e-25
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 226694210   3 ILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAI---WKTHHQPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEV 79
Cdd:PRK14659   2 IVGIGTDIVYIPRILNLLKKFGNKFLNRVFSEKEIEDslkYTSQEARARHFAKRFAAKEAYVKALGTGFGRGIKMKDISV 81

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*
gi 226694210  80 FNDELGKPRLRLWGEALKLaeklgvvNMHVTLADERHYACATVII 124
Cdd:PRK14659  82 YNDLYGKPQITVSKSNIDH-------KIELSLSDDGDYAIAFVVL 119
ACPS pfam01648
4'-phosphopantetheinyl transferase superfamily; Members of this family transfers the 4 ...
 5-112 4.41e-22

4'-phosphopantetheinyl transferase superfamily; Members of this family transfers the 4'-phosphopantetheine (4'-PP) moiety from coenzyme A (CoA) to the invariant serine of pfam00550. This post-translational modification renders holo-ACP capable of acyl group activation via thioesterification of the cysteamine thiol of 4'-PP. This superfamily consists of two subtypes: The ACPS type and the Sfp type. The structure of the Sfp type is known, which shows the active site accommodates a magnesium ion. The most highly conserved regions of the alignment are involved in binding the magnesium ion.


Pssm-ID: 426364 [Multi-domain]  Cd Length: 111  Bit Score: 83.81  E-value: 4.41e-22
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 226694210    5 GLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHHQPVR-FLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDE 83
Cdd:pfam01648   1 GVGIDIEEIARIRRPIERLGERLAERIFTPEERALLASLPAEARrAFARLWTAKEAVFKALGPGLSKLLDFDDIEVLLDP 80

                          90       100
                  ....*....|....*....|....*....
gi 226694210   84 LGKPRLRLWGEALKLAEKLGVVNMHVTLA 112
Cdd:pfam01648  81 DGRPTLRLLGEAADLAWRFEVLAGDYALA 109
acpS TIGR00516
holo-[acyl-carrier-protein] synthase; Formerly dpj. This enzyme adds the prosthetic group, ...
 5-126 3.96e-21

holo-[acyl-carrier-protein] synthase; Formerly dpj. This enzyme adds the prosthetic group, phosphopantethiene, to the acyl carrier protein (ACP) apo-enzyme to generate the holo-enzyme. Related phosphopantethiene--protein transferases also exist. There is an orthologous domain in eukaryotic proteins. [Fatty acid and phospholipid metabolism, Biosynthesis]


Pssm-ID: 273114 [Multi-domain]  Cd Length: 121  Bit Score: 81.66  E-value: 3.96e-21
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 226694210    5 GLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWK--THHQPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFND 82
Cdd:TIGR00516   1 GIGIDITEIARIAKCAGRFKKKFAERFLSPSEIDLCKdkSEKRKNEFIAGFFAAKEACSKAFGTGIGKELSFLDIEIRKD 80

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....
gi 226694210   83 ELGKPRLRLWGEALklaEKLGVVNMHVTLADERHYACATVIIES 126
Cdd:TIGR00516  81 PKGAPLITLSKEIC---DKFNIAALHASISHDAEFAAAQVVIER 121
acpS PRK14663
holo-[acyl-carrier-protein] synthase;
9-125 1.56e-17

holo-[acyl-carrier-protein] synthase;


Pssm-ID: 237781 [Multi-domain]  Cd Length: 116  Bit Score: 72.17  E-value: 1.56e-17
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 226694210   9 DIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHHQPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPR 88
Cdd:PRK14663   2 DIVDLERIEKAYNRYGVKFLEKILTPEEIELCLQKPQPVASIAGRFAAKEAVVKALGTGFSQGVHWKSFAILNDAAGRPF 81

                         90       100       110
                 ....*....|....*....|....*....|....*..
gi 226694210  89 LRLWGEALKLAEklGVVNmhVTLADERHYACATVIIE 125
Cdd:PRK14663  82 VKVIDDGCLPPG--CVIK--ISISHDRHSAVATALIE 114
acpS PRK14661
holo-[acyl-carrier-protein] synthase;
3-125 4.90e-14

holo-[acyl-carrier-protein] synthase;


Pssm-ID: 184782  Cd Length: 169  Bit Score: 64.56  E-value: 4.90e-14
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 226694210   3 ILGLGTDIVEIARIEaviarsgERLARRVLSDNEWAIWKTHHQPVRFLAKRFAVKEAAAKAFGTGIrNGLAFNQFEvFND 82
Cdd:PRK14661   2 IVGVGIDVLEVERVP-------EKFAERILGESEKRLFLTRKRRREFIAGRFALKEAFFKALGTGL-NGHSFTDVE-FLE 72

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....
gi 226694210  83 ELGKPrlrlwgeALKLAEKLGVVN-MHVTLADERhYACATVIIE 125
Cdd:PRK14661  73 SNGKP-------VLCVHKDFGFFNyAHVSLSHDR-FAVALVVLE 108
acpS PRK14662
4'-phosphopantetheinyl transferase; Provisional
 3-125 2.33e-11

4'-phosphopantetheinyl transferase; Provisional


Pssm-ID: 184783  Cd Length: 120  Bit Score: 56.34  E-value: 2.33e-11
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 226694210   3 ILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHHQPVRFLAKRFAVKEAAAKAFGTgirnglAFNQFEVFND 82
Cdd:PRK14662   2 IVAIGHDLVEIARIRRVLERHGERALERLFHPEELAYCLAKADPAPSLAARFAAKEAFQKCWPE------SHGWREVWVE 75

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*..
gi 226694210  83 -ELGKPRLRLwgeALKLAEKL---GVVnMHVTLADERHYACATVIIE 125
Cdd:PRK14662  76 rEGARPVLGF---APKIAARMeeeGWV-AHLSLSHEKEHALAVVVLE 118
Sfp COG2091
Phosphopantetheinyl transferase [Coenzyme transport and metabolism];
16-72 1.51e-05

Phosphopantetheinyl transferase [Coenzyme transport and metabolism];


Pssm-ID: 441694  Cd Length: 177  Bit Score: 41.87  E-value: 1.51e-05
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*....
gi 226694210  16 IEAVIARSGERLARRVLSDNEWAIWKTH--HQPVRFLAKRFAVKEAAAKAFGTGIRNGL 72
Cdd:COG2091  113 IERIRPRIDLALARRFFSPEERAWLAALpqDDRLEAFTRLWTLKEALLKATGTGLSLPL 171
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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