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Conserved domains on  [gi|1747300|dbj|BAA09052|]
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SDF4 [Mus musculus]

Protein Classification

CREC-EF hand family protein; EF-hand domain-containing protein( domain architecture ID 11610931)

CREC (Cab45/reticulocalbin/ERC45/calumenin)-EF hand family protein; the family consists of a group of six EF-hand, low-affinity Ca2+-binding proteins, including reticulocalbin (RCN-1), ER Ca2+-binding protein of 55, reticulocalbin-3 (RCN-3), cab45 Ca2+-binding protein, and calumenin (also known as crocalbin or CBP-50)| EF-hand (EFh) domain-containing protein may be involved in binding intracellular calcium and in calcium signal transduction

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
EFh_CREC_cab45 cd16225
EF-hand, calcium binding motif, found in 45 kDa calcium-binding protein (Cab45); Cab45, also ...
 66-346 1.66e-146

EF-hand, calcium binding motif, found in 45 kDa calcium-binding protein (Cab45); Cab45, also termed stromal cell-derived factor 4 (SDF-4), is a soluble, lumenal Golgi resident low-affinity Ca2+-binding protein that contains six copies of the EF-hand Ca2+-binding motif. It is required for secretory pathway calcium ATPase1 (SPCA1)-dependent Ca2+ import into the trans-Golgi network (TGN) and plays an essential role in Ca2+-dependent secretory cargo sorting at the TGN.


:

Pssm-ID: 320023 [Multi-domain]  Cd Length: 278  Bit Score: 415.16  E-value: 1.66e-146
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1747300   66 LEMDGHLNKDFHQEVFLGKDMDGFDEDSEPRRsRRKLMVIFSKVDVNTDRRISAKEMQHWIMEKTAEHFQEAVKENKLHF 145
Cdd:cd16225   1 LERDGHLNKEFHKEVFLGNEKEEFEEDSEPKK-RKKLKEIFKKVDVNTDGFLSAEELEDWIMEKTQEHFQEAVEENEQIF 79

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1747300  146 RAVDPDGDGHVSWDEYKVKFLASKGHNEREIAEAIKNHEELKVDEETQEVLGNLRDRWYQADnPPADLLLTEDEFLSFLH 225
Cdd:cd16225  80 KAVDTDKDGNVSWEEYRVHFLLSKGYSEEEAEEKIKNNEELKLDEDDKEVLDRYKDRWSQAD-EPEDGLLDVEEFLSFRH 158

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1747300  226 PEHSRGMLKFMVKEIFRDLDQDGDKQLSLPEFISLPVGTVENQQGQDiDDNWVKDRKKEFEELIDSNHDGIVTMEELENY 305
Cdd:cd16225 159 PEHSRGMLKNMVKEILHDLDQDGDEKLTLDEFVSLPPGTVEEQQAED-DDEWKKERKKEFEEVIDLNHDGKVTKEELEEY 237

                       250       260       270       280
                ....*....|....*....|....*....|....*....|.
gi 1747300  306 MDPMNEYNALNEAKQMIAIADENQNHHLEPEEILKYSEFFT 346
Cdd:cd16225 238 MDPRNERHALNEAKQLIAVADENKDGKLSLEEILKNSDLFT 278
 
Name Accession Description Interval E-value
EFh_CREC_cab45 cd16225
EF-hand, calcium binding motif, found in 45 kDa calcium-binding protein (Cab45); Cab45, also ...
 66-346 1.66e-146

EF-hand, calcium binding motif, found in 45 kDa calcium-binding protein (Cab45); Cab45, also termed stromal cell-derived factor 4 (SDF-4), is a soluble, lumenal Golgi resident low-affinity Ca2+-binding protein that contains six copies of the EF-hand Ca2+-binding motif. It is required for secretory pathway calcium ATPase1 (SPCA1)-dependent Ca2+ import into the trans-Golgi network (TGN) and plays an essential role in Ca2+-dependent secretory cargo sorting at the TGN.


Pssm-ID: 320023 [Multi-domain]  Cd Length: 278  Bit Score: 415.16  E-value: 1.66e-146
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1747300   66 LEMDGHLNKDFHQEVFLGKDMDGFDEDSEPRRsRRKLMVIFSKVDVNTDRRISAKEMQHWIMEKTAEHFQEAVKENKLHF 145
Cdd:cd16225   1 LERDGHLNKEFHKEVFLGNEKEEFEEDSEPKK-RKKLKEIFKKVDVNTDGFLSAEELEDWIMEKTQEHFQEAVEENEQIF 79

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1747300  146 RAVDPDGDGHVSWDEYKVKFLASKGHNEREIAEAIKNHEELKVDEETQEVLGNLRDRWYQADnPPADLLLTEDEFLSFLH 225
Cdd:cd16225  80 KAVDTDKDGNVSWEEYRVHFLLSKGYSEEEAEEKIKNNEELKLDEDDKEVLDRYKDRWSQAD-EPEDGLLDVEEFLSFRH 158

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1747300  226 PEHSRGMLKFMVKEIFRDLDQDGDKQLSLPEFISLPVGTVENQQGQDiDDNWVKDRKKEFEELIDSNHDGIVTMEELENY 305
Cdd:cd16225 159 PEHSRGMLKNMVKEILHDLDQDGDEKLTLDEFVSLPPGTVEEQQAED-DDEWKKERKKEFEEVIDLNHDGKVTKEELEEY 237

                       250       260       270       280
                ....*....|....*....|....*....|....*....|.
gi 1747300  306 MDPMNEYNALNEAKQMIAIADENQNHHLEPEEILKYSEFFT 346
Cdd:cd16225 238 MDPRNERHALNEAKQLIAVADENKDGKLSLEEILKNSDLFT 278
FRQ1 COG5126
Ca2+-binding protein, EF-hand superfamily [Signal transduction mechanisms];
215-340 5.69e-07

Ca2+-binding protein, EF-hand superfamily [Signal transduction mechanisms];


Pssm-ID: 444056 [Multi-domain]  Cd Length: 137  Bit Score: 48.25  E-value: 5.69e-07
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1747300  215 LTEDEFLSFLHpehsrgmlkFMVKEIFRDLDQDGDKQLSLPEFISLPVGTVENQQGQDIDdnwvkdrkKEFeELIDSNHD 294
Cdd:COG5126  22 LERDDFEALFR---------RLWATLFSEADTDGDGRISREEFVAGMESLFEATVEPFAR--------AAF-DLLDTDGD 83

                        90       100       110       120
                ....*....|....*....|....*....|....*....|....*.
gi 1747300  295 GIVTMEELENYMDPMNEYNAlnEAKQMIAIADENQNHHLEPEEILK 340
Cdd:COG5126  84 GKISADEFRRLLTALGVSEE--EADELFARLDTDGDGKISFEEFVA 127
EF-hand_7 pfam13499
EF-hand domain pair;
99-162 2.25e-05

EF-hand domain pair;


Pssm-ID: 463900 [Multi-domain]  Cd Length: 67  Bit Score: 41.86  E-value: 2.25e-05
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 1747300     99 RRKLMVIFSKVDVNTDRRISAKEMQHwIMEKTAEHFQEAVKENKLHFRAVDPDGDGHVSWDEYK 162
Cdd:pfam13499   1 EEKLKEAFKLLDSDGDGYLDVEELKK-LLRKLEEGEPLSDEEVEELFKEFDLDKDGRISFEEFL 63
 
Name Accession Description Interval E-value
EFh_CREC_cab45 cd16225
EF-hand, calcium binding motif, found in 45 kDa calcium-binding protein (Cab45); Cab45, also ...
 66-346 1.66e-146

EF-hand, calcium binding motif, found in 45 kDa calcium-binding protein (Cab45); Cab45, also termed stromal cell-derived factor 4 (SDF-4), is a soluble, lumenal Golgi resident low-affinity Ca2+-binding protein that contains six copies of the EF-hand Ca2+-binding motif. It is required for secretory pathway calcium ATPase1 (SPCA1)-dependent Ca2+ import into the trans-Golgi network (TGN) and plays an essential role in Ca2+-dependent secretory cargo sorting at the TGN.


Pssm-ID: 320023 [Multi-domain]  Cd Length: 278  Bit Score: 415.16  E-value: 1.66e-146
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1747300   66 LEMDGHLNKDFHQEVFLGKDMDGFDEDSEPRRsRRKLMVIFSKVDVNTDRRISAKEMQHWIMEKTAEHFQEAVKENKLHF 145
Cdd:cd16225   1 LERDGHLNKEFHKEVFLGNEKEEFEEDSEPKK-RKKLKEIFKKVDVNTDGFLSAEELEDWIMEKTQEHFQEAVEENEQIF 79

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1747300  146 RAVDPDGDGHVSWDEYKVKFLASKGHNEREIAEAIKNHEELKVDEETQEVLGNLRDRWYQADnPPADLLLTEDEFLSFLH 225
Cdd:cd16225  80 KAVDTDKDGNVSWEEYRVHFLLSKGYSEEEAEEKIKNNEELKLDEDDKEVLDRYKDRWSQAD-EPEDGLLDVEEFLSFRH 158

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1747300  226 PEHSRGMLKFMVKEIFRDLDQDGDKQLSLPEFISLPVGTVENQQGQDiDDNWVKDRKKEFEELIDSNHDGIVTMEELENY 305
Cdd:cd16225 159 PEHSRGMLKNMVKEILHDLDQDGDEKLTLDEFVSLPPGTVEEQQAED-DDEWKKERKKEFEEVIDLNHDGKVTKEELEEY 237

                       250       260       270       280
                ....*....|....*....|....*....|....*....|.
gi 1747300  306 MDPMNEYNALNEAKQMIAIADENQNHHLEPEEILKYSEFFT 346
Cdd:cd16225 238 MDPRNERHALNEAKQLIAVADENKDGKLSLEEILKNSDLFT 278
EFh_CREC cd15899
EF-hand, calcium binding motif, found in CREC-EF hand family; The CREC (Cab45/reticulocalbin ...
 66-346 1.65e-109

EF-hand, calcium binding motif, found in CREC-EF hand family; The CREC (Cab45/reticulocalbin/ERC45/calumenin)-EF hand family contains a group of six EF-hand, low-affinity Ca2+-binding proteins, including reticulocalbin (RCN-1), ER Ca2+-binding protein of 55 kDa (ERC-55, also known as TCBP-49 or E6BP), reticulocalbin-3 (RCN-3), Ca2+-binding protein of 45 kDa (Cab45 and its splice variant Cab45b), and calumenin ( also known as crocalbin or CBP-50). The proteins are not only localized in various parts of the secretory pathway, but also found in the cytosolic compartment and at the cell surface. They interact with different ligands or proteins and have been implicated in the secretory process, chaperone activity, signal transduction as well as in a large variety of disease processes.


Pssm-ID: 320021 [Multi-domain]  Cd Length: 267  Bit Score: 320.93  E-value: 1.65e-109
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1747300   66 LEMDGHLNKDFHQEVFLGKDMDGFDEDSEPRRSRRKLMVIFSKVDVNTDRRISAKEMQHWIMEKTAEHFQEAVKEnklHF 145
Cdd:cd15899   1 HEMDGHLNSDYDHEAFLGKEEAEEFDQLTPEESKRRLGVIVSKMDVDKDGFISAKELHSWILESFKRHAMEESKE---QF 77

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1747300  146 RAVDPDGDGHVSWDEYKVKFLASKGHNEREIAEAIKnheelkVDEETQEVLGNLRDRWYQADnPPADLLLTEDEFLSFLH 225
Cdd:cd15899  78 RAVDPDEDGHVSWDEYKNDTYGSVGDDEENVADNIK------EDEEYKKLLLKDKKRFEAAD-QDGDLILTLEEFLAFLH 150

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1747300  226 PEHSRGMLKFMVKEIFRDLDQDGDKQLSLPEFISLPVGTVENQQGQdiddNWVKDRKKEFEELIDSNHDGIVTMEELENY 305
Cdd:cd15899 151 PEESPYMLDFVIKETLEDLDKNGDGFISLEEFISDPYSADENEEEP----EWVKVEKERFVELRDKDKDGKLDGEELLSW 226

                       250       260       270       280
                ....*....|....*....|....*....|....*....|.
gi 1747300  306 MDPMNEYNALNEAKQMIAIADENQNHHLEPEEILKYSEFFT 346
Cdd:cd15899 227 VDPSNQEIALEEAKHLIAESDENKDGKLSPEEILDNHELFV 267
EFh_CREC_Calumenin_like cd16226
EF-hand, calcium binding motif, found in calumenin, reticulocalbin-1 (RCN-1), reticulocalbin-3 ...
 69-347 1.19e-42

EF-hand, calcium binding motif, found in calumenin, reticulocalbin-1 (RCN-1), reticulocalbin-3 (RCN-3), and similar proteins; The family corresponds to a group of six EF-hand Ca2+-binding proteins, including calumenin (also known as crocalbin or CBP-50), reticulocalbin-1 (RCN-1), reticulocalbin-3 (RCN-3), and similar proteins. Calumenin is an endo/sarcoplasmic reticulum (ER/SR) resident low-affinity Ca2+-binding protein that contains six EF-hand domains and a C-terminal SR retention signal His-Asp-Glu-Phe (HDEF) tetrapeptide. It functions as a novel regulator of SERCA2, and its expressional changes are tightly coupled with Ca2+-cycling of cardiomyocytes. It is also broadly involved in haemostasis and in the pathophysiology of thrombosis. Moreover, the extracellular calumenin acts as a suppressor of cell migration and tumor metastasis. RCN-1 is an endoplasmic reticulum resident Ca2+-binding protein with a carboxyl-terminal His-Asp-Glu-Leu (HDEL) tetrapeptide signal. It acts as a potential negative regulator of B-RAF activation and can negatively modulate cardiomyocyte hypertrophy by inhibition of the mitogen-activated protein kinase signalling cascade. It also plays a key role in the development of doxorubicin-associated resistance. RCN-3 is a putative six EF-hand Ca2+-binding protein that contains five RXXR (X is any amino acid) motifs and a C-terminal ER retrieval signal HDEL tetrapeptide. The RXXR motif represents the target sequence of subtilisin-like proprotein convertases (SPCs). RCN-3 is specifically bound to the paired basic amino-acid-cleaving enzyme-4 (PACE4) precursor protein and plays an important role in the biosynthesis of PACE4.


Pssm-ID: 320024 [Multi-domain]  Cd Length: 264  Bit Score: 148.89  E-value: 1.19e-42
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1747300   69 DGHLNKDFHQEVFLGKDMDG-FDEDSePRRSRRKLMVIFSKVDVNTDRRISAKEMQHWIMEKTAEHFQEAVKEnklHFRA 147
Cdd:cd16226   4 DGEHNPEYDHEAFLGKEEAKeFDQLT-PEESKERLGIIVDKIDKNGDGFVTEEELKDWIKYVQKKYIREDVDR---QWKE 79

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1747300  148 VDPDGDGHVSWDEYKVKFLASKGHNEREIAEAIKNHEELKVDEEtqevlgnlrdRWYQADNPpADLLLTEDEFLSFLHPE 227
Cdd:cd16226  80 YDPNKDGKLSWEEYKKATYGFLDDEEEDDDLHESYKKMIRRDER----------RWKAADQD-GDGKLTKEEFTAFLHPE 148

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1747300  228 HSRGMLKFMVKEIFRDLDQDGDKQLSLPEFIslpvGTVENQQGQDIDDNWVKDRKKEFEELIDSNHDGIVTMEELENYMD 307
Cdd:cd16226 149 EFPHMRDIVVQETLEDIDKNKDGFISLEEYI----GDMYRDDDEEEDPDWVKSEREQFKEFRDKNKDGKMDREEVKDWIL 224

                       250       260       270       280
                ....*....|....*....|....*....|....*....|
gi 1747300  308 PMNEYNALNEAKQMIAIADENQNHHLEPEEILKYSEFFTG 347
Cdd:cd16226 225 PEDYDHAEAEAKHLIYEADDDKDGKLTKEEILDKYDLFVG 264
EFh_CREC_RCN2_like cd16227
EF-hand, calcium binding motif, found in reticulocalbin-2 (RCN2) mainly from protostomes; This ...
 69-347 3.66e-29

EF-hand, calcium binding motif, found in reticulocalbin-2 (RCN2) mainly from protostomes; This family corresponds to a group of uncharacterized RCN2-like proteins, which are mainly found in protostomes. Although their biological function remains unclear, they show high sequence similarity with RCN2 (also known as E6BP or TCBP-49), which is an endoplasmic reticulum resident low-affinity Ca2+-binding protein that has been implicated in immunity, redox homeostasis, cell cycle regulation and coagulation. Members in this family contain six copies of the EF-hand Ca2+-binding motif, but may lack a C-terminal His-Asp-Glu-Leu (HDEL) tetrapeptide that is required for retention of RCN2 in the endoplasmic reticulum (ER).


Pssm-ID: 320025 [Multi-domain]  Cd Length: 263  Bit Score: 113.18  E-value: 3.66e-29
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1747300   69 DGHLNKDFHQEVFLG--KDMDGFDEDSePRRSRRKLMVIFSKVDVNTDRRISAKEMQHWIMEKTAEHFQEAVKENklhFR 146
Cdd:cd16227   4 DGEHNPEFDHEAVLGsrKEAEEFDELP-PEEAKRRLAVLAKKMDLNDDGFIDRKELKAWILRSFKMLDEEEANER---FE 79

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1747300  147 AVDPDGDGHVSWDEYKVKflaSKGHNEREIAEAIKNHeelkvDEETQEVLGNLRDRWYQADNPpADLLLTEDEFLSFLHP 226
Cdd:cd16227  80 EADEDGDGKVTWEEYLAD---SFGYDDEDNEEMIKDS-----TEDDLKLLEDDKEMFEAADLN-KDGKLDKTEFSAFQHP 150

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1747300  227 EHSRGMLKFMVKEIFRDLDQDGDKQLSLPEFISlpvgtvenQQGQDIDDNWVKDRKKEFEELIDSNHDGIVTMEELENYM 306
Cdd:cd16227 151 EEYPHMHPVLIEQTLRDKDKDNDGFISFQEFLG--------DRAGHEDKEWLLVEKDRFDEDYDKDGDGKLDGEEILSWL 222

                       250       260       270       280
                ....*....|....*....|....*....|....*....|.
gi 1747300  307 DPMNEYNALNEAKQMIAIADENQNHHLEPEEILKYSEFFTG 347
Cdd:cd16227 223 VPDNEEIAEEEVDHLFASADDDHDDRLSFDEILDHHEIFVG 263
EFh_CREC_Calumenin cd16228
EF-hand, calcium binding motif, found in calumenin; Calumenin, also termed crocalbin, or IEF ...
 69-347 3.57e-28

EF-hand, calcium binding motif, found in calumenin; Calumenin, also termed crocalbin, or IEF SSP 9302, is an endo/sarcoplasmic reticulum (ER/SR) resident low-affinity Ca2+-binding protein that contains six EF-hand domains and a C-terminal SR retention signal His-Asp-Glu-Phe (HDEF) tetrapeptide. It is highly expressed in various brain regions. Thus it plays an important role in migration and differentiation of neurons, and/or in Ca2+ signaling between glial cells and neurons. Calumenin is involved in Ca2+ homeostasis through interacting with ryanodine receptor RyR2 and SERCA2. It acts as a novel regulator of SERCA2, and its expressional changes are tightly coupled with Ca2+-cycling of cardiomyocytes. Calumenin also forms a Ca2+-dependent complex with thrombospondin-1, which is broadly involved in haemostasis and thrombosis. Moreover, calumenin is a molecular chaperone that endogenously regulates the vitamin K-dependent gamma-carboxylation of several proteins, including blood coagulation factors (such as FII, FVII, FIX, FX, and proteins C, S and Z), cell survival factors (Gas6) and bone metabolism proteins (such as matrix Gla protein or MGP, osteocalcin and periostin), through targeting the gamma-glutamyl carboxylase. It also functions as a charged F508del-cystic fibrosis transmembrane regulator (CFTR) folding modulator, as well as a G551D-CFTR associated protein. Furthermore, the extracellular calumenin acts as a suppressor of cell migration and tumor metastasis. It binds to and stabilizes fibulin-1, and further inactivates extracellular signal-regulated kinases 1 and 2 (ERK1/2) signaling.


Pssm-ID: 320026 [Multi-domain]  Cd Length: 263  Bit Score: 110.80  E-value: 3.57e-28
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1747300   69 DGHLNKDFHQEVFLG-KDMDGFDEDSePRRSRRKLMVIFSKVDVNTDRRISAKEMQHWIMEKTAEHFQEAVKEnklHFRA 147
Cdd:cd16228   4 DDAQNFDYDHDAFLGaEEAKTFDQLT-PEESKERLGKIVGKIDEDKDGFVTEDELKAWIKFAQKRWIYEDVER---QWKG 79

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1747300  148 VDPDGDGHVSWDEYKVkflASKGHN-EREIAEAIKNHEELKVDEEtqevlgnlrdRWYQADNPPADLLLTEDEFLSFLHP 226
Cdd:cd16228  80 HDLNEDGLVSWEEYKN---ATYGYIlDDPDPDDGFNYKQMMVRDE----------RRFKMADKDGDLRATKEEFTAFLHP 146

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1747300  227 EHSRGMLKFMVKEIFRDLDQDGDKQLSLPEFIslpvGTVENQQGQDIDDNWVKDRKKEFEELIDSNHDGIVTMEELENYM 306
Cdd:cd16228 147 EEYDYMKDIVVLETMEDIDKNGDGFIDLEEYI----GDMYSQDGDADEPEWVKTEREQFTEFRDKNKDGKMDKEETKDWI 222

                       250       260       270       280
                ....*....|....*....|....*....|....*....|.
gi 1747300  307 DPMNEYNALNEAKQMIAIADENQNHHLEPEEILKYSEFFTG 347
Cdd:cd16228 223 LPSDYDHAEAEARHLVYESDQNKDGKLTKEEIVDKYDLFVG 263
EFh_CREC_RCN2 cd16224
EF-hand, calcium binding motif, found in reticulocalbin-2 (RCN2); RCN2, also termed ...
 68-345 2.16e-27

EF-hand, calcium binding motif, found in reticulocalbin-2 (RCN2); RCN2, also termed calcium-binding protein ERC-55, or E6-binding protein (E6BP), or TCBP-49, is an endoplasmic reticulum resident low-affinity Ca2+-binding protein that has been implicated in immunity, redox homeostasis, cell cycle regulation and coagulation. It is associated with tumorigenesis, in particular with transformation of cells of the cervix induced by human papillomavirus (HPV), through binding to human papillomavirus (HPV) E6 oncogenic protein. It specifically interacts with vitamin D receptor among nuclear receptors. RCN2 contains an N-terminal signal sequence followed by six copies of the EF-hand Ca2+-binding motif, and a C-terminal His-Asp-Glu-Leu (HDEL) tetrapeptide that is required for retention of RCN2 in the endoplasmic reticulum (ER).


Pssm-ID: 320022 [Multi-domain]  Cd Length: 268  Bit Score: 108.67  E-value: 2.16e-27
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1747300   68 MDGHLNKDFHQEVFLG--KDMDGFDEDSePRRSRRKLMVIFSKVDVNTDRRISAKEMQHWImEKTAEHFqeAVKENKLHF 145
Cdd:cd16224   3 PNGEHNAEYDKEAFLGgeEDADEFAKLS-PEEQQKRLKSIIKKIDTDSDGFLTEEELSSWI-QQSFRHY--ALEDAKQQF 78

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1747300  146 RAVDPDGDGHVSWDEYKVkflaskghnerEIAEAIKNHEELKVDEETQEV---LGNLRD-RWYQADNPPADLLLTEDEFL 221
Cdd:cd16224  79 PEYDKDGDGAVTWDEYNM-----------QMYDRVIDYDEDTVLDDEEEEsfrQLHLKDkKRFDKANTDGGPGLNLTEFI 147

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1747300  222 SFLHPEHSRGMLKFMVKEIFRDLDQDGDKQLSLPEFIslpvGTVENQQGQDIDDNWVKDRKKEFEELIDSNHDGIVTMEE 301
Cdd:cd16224 148 AFEHPEEVDYMTEFVIQEALEEHDKDGDGFISLEEFL----GDYRKDPTANEDPEWIIVEKDRFVNDYDKDNDGKLDPQE 223

                       250       260       270       280
                ....*....|....*....|....*....|....*....|....
gi 1747300  302 LENYMDPMNEYNALNEAKQMIAIADENQNHHLEPEEILKYSEFF 345
Cdd:cd16224 224 LLPWVVPNNYGIAQEEALHLIDEMDLNGDGRLSEEEILENQDLF 267
EFh_CREC_RCN3 cd16230
EF-hand, calcium binding motif, found in reticulocalbin-3 (RCN-3); RCN-3, also termed EF-hand ...
 69-347 6.17e-24

EF-hand, calcium binding motif, found in reticulocalbin-3 (RCN-3); RCN-3, also termed EF-hand calcium-binding protein RLP49, is a putative six EF-hand Ca2+-binding protein that contains five RXXR (X is any amino acid) motifs and a C-terminal ER retrieval signal His-Asp-Glu-Leu (HDEL) tetrapeptide. The RXXR motif represents the target sequence of subtilisin-like proprotein convertases (SPCs). RCN-3 is specifically bound to the paired basic amino-acid-cleaving enzyme-4 (PACE4) precursor protein and plays an important role in the biosynthesis of PACE4.


Pssm-ID: 320028 [Multi-domain]  Cd Length: 268  Bit Score: 99.28  E-value: 6.17e-24
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1747300   69 DGHLNKDFHQEVFLGKDM-DGFDEDSePRRSRRKLMVIFSKVDV--NTDRRISAKEMQHWIMEKTAEHFQEAVKENklhF 145
Cdd:cd16230   4 DAHGNFQYDHEAFLGREVaKEFDQLS-PEESQARLGRIVDRMDRagDGDGWVSLAELRAWIAHTQQRHIRDSVSAA---W 79

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1747300  146 RAVDPDGDGHVSWDEYKVkflASKGHNEREiaEAIKNHEelkvDEETQEVLGNLRDRWYQADNPPADLLLTEDEFLSFLH 225
Cdd:cd16230  80 QTYDTDRDGRVGWEELRN---ATYGHYEPG--EEFHDVE----DAETYKKMLARDERRFRVADQDGDSMATREELTAFLH 150

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1747300  226 PEHSRGMLKFMVKEIFRDLDQDGDKQLSLPEFIS-LPVGTVENQQGQdiddnWVKDRKKEFEELIDSNHDGIVTMEELEN 304
Cdd:cd16230 151 PEEFPHMRDIVVAETLEDLDKNKDGYVQVEEYIAdLYSGEPGEEEPA-----WVQTERQQFRQFRDLNKDGRLDGSEVGH 225

                       250       260       270       280
                ....*....|....*....|....*....|....*....|...
gi 1747300  305 YMDPMNEYNALNEAKQMIAIADENQNHHLEPEEILKYSEFFTG 347
Cdd:cd16230 226 WVLPPSQDQPLVEANHLLHESDTDKDGRLSKAEILGNWNMFVG 268
EFh_CREC_RCN1 cd16229
EF-hand, calcium binding motif, found in reticulocalbin-1 (RCN-1); RCN-1 is an endoplasmic ...
 73-347 1.21e-23

EF-hand, calcium binding motif, found in reticulocalbin-1 (RCN-1); RCN-1 is an endoplasmic reticulum resident low-affinity Ca2+-binding protein with six EF-hand motifs and a carboxyl-terminal His-Asp-Glu-Leu (HDEL) tetrapeptide signal. It is expressed at the cell surface. RCN-1 acts as a potential negative regulator of B-RAF activation and can negatively modulate cardiomyocyte hypertrophy by inhibition of the mitogen-activated protein kinase signaling cascade. It also plays a key role in the development of doxorubicin-associated resistance.


Pssm-ID: 320027 [Multi-domain]  Cd Length: 267  Bit Score: 98.41  E-value: 1.21e-23
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1747300   73 NKDFHQ--EVFLGKDMDGFDEDSEPRRSRRKLMVIFSKVDVNTDRRISAKEMQHWIMEKTAEHFQEAVKENklhFRAVDP 150
Cdd:cd16229   6 NQSFQYdhEAFLGKEEAKTFDQLTPEESKERLGKIVDRIDDDKDGFVTTEELKAWIKRVQKRYIYENVAKV---WKDYDL 82

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1747300  151 DGDGHVSWDEYKVkflASKGHNEREIAEAIKNHEELKVdeetQEVLGNLRDRWYQADNPpADLLLTEDEFLSFLHPEHSR 230
Cdd:cd16229  83 NKDNKISWEEYKQ---ATYGYYLGNPEEFQDATDQFSF----KKMLPRDERRFKAADLD-GDLAATREEFTAFLHPEEFE 154

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1747300  231 GMLKFMVKEIFRDLDQDGDKQLSLPEFISLPVGTVENQQGQDiddnWVKDRKKEFEELIDSNHDGIVTMEELENYMDPMN 310
Cdd:cd16229 155 HMKDIVVLETLEDIDKNGDGFVDEDEYIADMFSHEEGGPEPD----WVKTEREQFSDFRDLNKDGKMDKEEIRHWILPQD 230

                       250       260       270
                ....*....|....*....|....*....|....*..
gi 1747300  311 EYNALNEAKQMIAIADENQNHHLEPEEILKYSEFFTG 347
Cdd:cd16229 231 YDHAQAEARHLVYESDKDKDQKLTKEEILDNWNMFVG 267
FRQ1 COG5126
Ca2+-binding protein, EF-hand superfamily [Signal transduction mechanisms];
215-340 5.69e-07

Ca2+-binding protein, EF-hand superfamily [Signal transduction mechanisms];


Pssm-ID: 444056 [Multi-domain]  Cd Length: 137  Bit Score: 48.25  E-value: 5.69e-07
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1747300  215 LTEDEFLSFLHpehsrgmlkFMVKEIFRDLDQDGDKQLSLPEFISLPVGTVENQQGQDIDdnwvkdrkKEFeELIDSNHD 294
Cdd:COG5126  22 LERDDFEALFR---------RLWATLFSEADTDGDGRISREEFVAGMESLFEATVEPFAR--------AAF-DLLDTDGD 83

                        90       100       110       120
                ....*....|....*....|....*....|....*....|....*.
gi 1747300  295 GIVTMEELENYMDPMNEYNAlnEAKQMIAIADENQNHHLEPEEILK 340
Cdd:COG5126  84 GKISADEFRRLLTALGVSEE--EADELFARLDTDGDGKISFEEFVA 127
FRQ1 COG5126
Ca2+-binding protein, EF-hand superfamily [Signal transduction mechanisms];
89-160 4.13e-06

Ca2+-binding protein, EF-hand superfamily [Signal transduction mechanisms];


Pssm-ID: 444056 [Multi-domain]  Cd Length: 137  Bit Score: 45.94  E-value: 4.13e-06
                        10        20        30        40        50        60        70
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 1747300   89 FDEDSEPRRSRRKLMVIFSKVDVNTDRRISAKEMQHWI--MEKTAEHFQEAvkenklhFRAVDPDGDGHVSWDE 160
Cdd:COG5126  58 GMESLFEATVEPFARAAFDLLDTDGDGKISADEFRRLLtaLGVSEEEADEL-------FARLDTDGDGKISFEE 124
FRQ1 COG5126
Ca2+-binding protein, EF-hand superfamily [Signal transduction mechanisms];
239-341 8.75e-06

Ca2+-binding protein, EF-hand superfamily [Signal transduction mechanisms];


Pssm-ID: 444056 [Multi-domain]  Cd Length: 137  Bit Score: 44.78  E-value: 8.75e-06
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1747300  239 EIFRDLDQDGDKQLSLPEFISLPVGTVENQQGQdiddnwvkdrkkefeelIDSNHDGIVTMEELENYMDPMNEYNALNEA 318
Cdd:COG5126   9 RRFDLLDADGDGVLERDDFEALFRRLWATLFSE-----------------ADTDGDGRISREEFVAGMESLFEATVEPFA 71

                        90       100
                ....*....|....*....|...
gi 1747300  319 KQMIAIADENQNHHLEPEEILKY 341
Cdd:COG5126  72 RAAFDLLDTDGDGKISADEFRRL 94
FRQ1 COG5126
Ca2+-binding protein, EF-hand superfamily [Signal transduction mechanisms];
99-260 2.14e-05

Ca2+-binding protein, EF-hand superfamily [Signal transduction mechanisms];


Pssm-ID: 444056 [Multi-domain]  Cd Length: 137  Bit Score: 43.63  E-value: 2.14e-05
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1747300   99 RRKLMVIFSKVDVNTDRRISAKEMqhwimEKTAEHFQEAVkenklhFRAVDPDGDGHVSWDEYKVKFLASKGHNEREIAE 178
Cdd:COG5126   4 RRKLDRRFDLLDADGDGVLERDDF-----EALFRRLWATL------FSEADTDGDGRISREEFVAGMESLFEATVEPFAR 72

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1747300  179 AIKNheelKVDeetqevlgnlRDRwyqadnppaDLLLTEDEFLSFLHpehSRGMLKFMVKEIFRDLDQDGDKQLSLPEFI 258
Cdd:COG5126  73 AAFD----LLD----------TDG---------DGKISADEFRRLLT---ALGVSEEEADELFARLDTDGDGKISFEEFV 126


                ..
gi 1747300  259 SL 260
Cdd:COG5126 127 AA 128
EF-hand_7 pfam13499
EF-hand domain pair;
99-162 2.25e-05

EF-hand domain pair;


Pssm-ID: 463900 [Multi-domain]  Cd Length: 67  Bit Score: 41.86  E-value: 2.25e-05
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 1747300     99 RRKLMVIFSKVDVNTDRRISAKEMQHwIMEKTAEHFQEAVKENKLHFRAVDPDGDGHVSWDEYK 162
Cdd:pfam13499   1 EEKLKEAFKLLDSDGDGYLDVEELKK-LLRKLEEGEPLSDEEVEELFKEFDLDKDGRISFEEFL 63
EFh cd00051
EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal ...
 101-160 2.21e-04

EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal modulators; most examples in this alignment model have 2 active canonical EF hands. Ca2+ binding induces a conformational change in the EF-hand motif, leading to the activation or inactivation of target proteins. EF-hands tend to occur in pairs or higher copy numbers.


Pssm-ID: 238008 [Multi-domain]  Cd Length: 63  Bit Score: 39.07  E-value: 2.21e-04
                        10        20        30        40        50        60
                ....*....|....*....|....*....|....*....|....*....|....*....|
gi 1747300  101 KLMVIFSKVDVNTDRRISAKEMQHWIMEKTAEHFQEAVKEnklHFRAVDPDGDGHVSWDE 160
Cdd:cd00051   1 ELREAFRLFDKDGDGTISADELKAALKSLGEGLSEEEIDE---MIREVDKDGDGKIDFEE 57
EFh cd00051
EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal ...
 287-340 8.09e-04

EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal modulators; most examples in this alignment model have 2 active canonical EF hands. Ca2+ binding induces a conformational change in the EF-hand motif, leading to the activation or inactivation of target proteins. EF-hands tend to occur in pairs or higher copy numbers.


Pssm-ID: 238008 [Multi-domain]  Cd Length: 63  Bit Score: 37.14  E-value: 8.09e-04
                        10        20        30        40        50
                ....*....|....*....|....*....|....*....|....*....|....
gi 1747300  287 ELIDSNHDGIVTMEELENYMDPMNEYNALNEAKQMIAIADENQNHHLEPEEILK 340
Cdd:cd00051   7 RLFDKDGDGTISADELKAALKSLGEGLSEEEIDEMIREVDKDGDGKIDFEEFLE 60
EFh_HEF cd15902
EF-hand, calcium binding motif, found in the hexa-EF hand proteins family; The hexa-EF hand ...
 239-308 1.20e-03

EF-hand, calcium binding motif, found in the hexa-EF hand proteins family; The hexa-EF hand proteins family, also named the calbindin sub-family, contains a group of six EF-hand Ca2+-binding proteins, including calretinin (CR, also termed 29 kDa calbindin), calbindin D28K (CB, also termed vitamin D-dependent calcium-binding protein, avian-type), and secretagogin (SCGN). CR is a cytosolic hexa-EF-hand calcium-binding protein predominantly expressed in a variety of normal and tumorigenic t-specific neurons of the central and peripheral nervous system. It is a multifunctional protein implicated in many biological processes, including cell proliferation, differentiation, and cell death. CB is highly expressed in brain tissue. It is a strong calcium-binding and buffering protein responsible for preventing a neuronal death as well as maintaining and controlling calcium homeostasis. SCGN is a six EF-hand calcium-binding protein expressed in neuroendocrine, pancreatic endocrine and retinal cells. It plays a crucial role in cell apoptosis, receptor signaling and differentiation. It is also involved in vesicle secretion through binding to various proteins, including interacts with SNAP25, SNAP23, DOC2alpha, ARFGAP2, rootletin, KIF5B, beta-tubulin, DDAH-2, ATP-synthase and myeloid leukemia factor 2. SCGN functions as a Ca2+ sensor/coincidence detector modulating vesicular exocytosis of neurotransmitters, neuropeptides or hormones. Although the family members share a significant amount of secondary sequence homology, they display altered structural and biochemical characteristics, and operate in distinct fashions. CB contains six EF-hand motifs in a single globular domain, where EF-hands 1, 3, 4, 5 bind four calcium ions. CR contains six EF-hand motifs within two independent domains, CR I-II and CR III-VI. They harbor two and four EF-hand motifs, respectively. The first 5 EF-hand motifs are capable of binding calcium ions, while the EF-hand 6 is inactive. SCGN consists of the three globular domains each of which contains a pair of EF-hand motifs. Human SCGN simultaneously binds four calcium ions through its EF-hands 3, 4, 5 and 6 in one high affinity and three low affinity calcium-binding sites. In contrast, SCGNs in other lower eukaryotes, such as D. rerio, X. laevis, M. domestica, G. gallus, O. anatinus, are fully competent in terms of six calcium-binding.


Pssm-ID: 320075 [Multi-domain]  Cd Length: 254  Bit Score: 40.03  E-value: 1.20e-03
                        10        20        30        40        50        60        70
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1747300  239 EIFRDLDQDGDKQLSLPEFISLPVGTVENQQGQDIDDNWVKDRKKEFEELIDSNHDGIVTMEELENYMDP 308
Cdd:cd15902   3 EVWMHFDADGNGYIEGKELDSFLRELLKALNGKDKTDDEVAEKKKEFMEKYDENEDGKIEIRELANILPT 72
EFh cd00051
EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal ...
 236-302 2.32e-03

EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal modulators; most examples in this alignment model have 2 active canonical EF hands. Ca2+ binding induces a conformational change in the EF-hand motif, leading to the activation or inactivation of target proteins. EF-hands tend to occur in pairs or higher copy numbers.


Pssm-ID: 238008 [Multi-domain]  Cd Length: 63  Bit Score: 35.99  E-value: 2.32e-03
                        10        20        30        40        50        60
                ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 1747300  236 MVKEIFRDLDQDGDKQLSLPEFISLPvgtvenqqgQDIDDNWVKDRKKEFEELIDSNHDGIVTMEEL 302
Cdd:cd00051   1 ELREAFRLFDKDGDGTISADELKAAL---------KSLGEGLSEEEIDEMIREVDKDGDGKIDFEEF 58
EFh cd00051
EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal ...
 215-260 2.51e-03

EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal modulators; most examples in this alignment model have 2 active canonical EF hands. Ca2+ binding induces a conformational change in the EF-hand motif, leading to the activation or inactivation of target proteins. EF-hands tend to occur in pairs or higher copy numbers.


Pssm-ID: 238008 [Multi-domain]  Cd Length: 63  Bit Score: 35.99  E-value: 2.51e-03
                        10        20        30        40
                ....*....|....*....|....*....|....*....|....*.
gi 1747300  215 LTEDEFLSFLhPEHSRGMLKFMVKEIFRDLDQDGDKQLSLPEFISL 260
Cdd:cd00051  17 ISADELKAAL-KSLGEGLSEEEIDEMIREVDKDGDGKIDFEEFLEL 61
EF-hand_7 pfam13499
EF-hand domain pair;
212-260 3.28e-03

EF-hand domain pair;


Pssm-ID: 463900 [Multi-domain]  Cd Length: 67  Bit Score: 35.69  E-value: 3.28e-03
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|
gi 1747300    212 DLLLTEDEFLSFLHP-EHSRGMLKFMVKEIFRDLDQDGDKQLSLPEFISL 260
Cdd:pfam13499  16 DGYLDVEELKKLLRKlEEGEPLSDEEVEELFKEFDLDKDGRISFEEFLEL 65
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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