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Conserved domains on  [gi|193788219|dbj|BAG53113|]
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unnamed protein product [Homo sapiens]

Protein Classification

MFS transporter( domain architecture ID 999995)

major facilitator superfamily (MFS) transporter facilitates the transport across cytoplasmic or internal membranes of one or more from a variety of substrates including ions, sugar phosphates, drugs, neurotransmitters, nucleosides, amino acids, and peptides

CATH:  1.20.1250.20
Gene Ontology:  GO:0022857|GO:0055085
PubMed:  26758938|26098515
SCOP:  3000310
TCDB:  2.A.1

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
MFS super family cl28910
Major Facilitator Superfamily; The Major Facilitator Superfamily (MFS) is a large and diverse ...
 1-219 8.75e-128

Major Facilitator Superfamily; The Major Facilitator Superfamily (MFS) is a large and diverse group of secondary transporters that includes uniporters, symporters, and antiporters. MFS proteins facilitate the transport across cytoplasmic or internal membranes of a variety of substrates including ions, sugar phosphates, drugs, neurotransmitters, nucleosides, amino acids, and peptides. They do so using the electrochemical potential of the transported substrates. Uniporters transport a single substrate, while symporters and antiporters transport two substrates in the same or in opposite directions, respectively, across membranes. MFS proteins are typically 400 to 600 amino acids in length, and the majority contain 12 transmembrane alpha helices (TMs) connected by hydrophilic loops. The N- and C-terminal halves of these proteins display weak similarity and may be the result of a gene duplication/fusion event. Based on kinetic studies and the structures of a few bacterial superfamily members, GlpT (glycerol-3-phosphate transporter), LacY (lactose permease), and EmrD (multidrug transporter), MFS proteins are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement. Bacterial members function primarily for nutrient uptake, and as drug-efflux pumps to confer antibiotic resistance. Some MFS proteins have medical significance in humans such as the glucose transporter Glut4, which is impaired in type II diabetes, and glucose-6-phosphate transporter (G6PT), which causes glycogen storage disease when mutated.


The actual alignment was detected with superfamily member cd17348:

Pssm-ID: 475125 [Multi-domain]  Cd Length: 435  Bit Score: 368.35  E-value: 8.75e-128
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 193788219   1 MQTTYVLQSLHLRIPEI-----SNITTTPHTLPAAWLTMFDAVLILLLIPLKDKLVDPILRRHGLLPSSLKRIAVGMFFV 75
Cdd:cd17348  211 MQTTYVLQSLHLKIPNIfisniAGITNSSHTFPAAWLTMFDAVLILILIPLKDKLVDPFLKRRGLLPSSLKRIAVGMFFV 290

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 193788219  76 MCSAFAAGILESKRLNLVKEKTINQT-IGNVVYHAADLSLWWQVPQYLLIGISEIFASIAGLEFAYSAAPKSMQSAIMGL 154
Cdd:cd17348  291 MCSAIAAGILESKRLEIVKHNTTIQQqIGNVTYYAADLPIWWQIPQYLLIGISEIFASIAGLEFAYSAAPKSMQSAIMGL 370

                        170       180       190       200       210       220
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 193788219 155 FFFFSGVGSFVGSGLLALVSIKAIGWMSSHTDFGNINGCYLNYYFFLLAAIQGATLLLFLIISVK 219
Cdd:cd17348  371 FFFFSGVGSFVGSGLLALVSIKEIGWMSSHKDFGNINGCRLNYYFFLLAGIQGVTLLLFLIISVK 435
 
Name Accession Description Interval E-value
MFS_SLC15A3_4 cd17348
Solute Carrier family 15 members 3 and 4 of the Major Facilitator Superfamily of transporters; ...
 1-219 8.75e-128

Solute Carrier family 15 members 3 and 4 of the Major Facilitator Superfamily of transporters; Solute carrier family 15 members 3 (SLC15A3) and 4 (SLC15A4) are members of the proton-coupled oligopeptide transporter (POT) family of integral membrane proteins that mediate the cellular uptake of di/tripeptides and peptide-like drugs. They are peptide/histidine transporters (PHTs) that transport free histidine in addition to di/tripeptides. SLC15A4, also called peptide transporter 4 or peptide/histidine transporter 1 (PHT1), is expressed in the human brain, retina, placenta, and immune cells. It is required for Toll-like receptor 7 (TLR7)- and TLR9-mediated type I interferon production in plasmacytoid dendritic cells (pDCs) and is involved in the pathogenesis of lupus-like autoimmunity. SLC15A3, also called osteoclast transporter, peptide transporter 3, or peptide/histidine transporter 2 (PHT2), is expressed in immune tissues including the spleen and thymus. The SLC15A3/SLC15A4 group belongs to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 340906 [Multi-domain]  Cd Length: 435  Bit Score: 368.35  E-value: 8.75e-128
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 193788219   1 MQTTYVLQSLHLRIPEI-----SNITTTPHTLPAAWLTMFDAVLILLLIPLKDKLVDPILRRHGLLPSSLKRIAVGMFFV 75
Cdd:cd17348  211 MQTTYVLQSLHLKIPNIfisniAGITNSSHTFPAAWLTMFDAVLILILIPLKDKLVDPFLKRRGLLPSSLKRIAVGMFFV 290

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 193788219  76 MCSAFAAGILESKRLNLVKEKTINQT-IGNVVYHAADLSLWWQVPQYLLIGISEIFASIAGLEFAYSAAPKSMQSAIMGL 154
Cdd:cd17348  291 MCSAIAAGILESKRLEIVKHNTTIQQqIGNVTYYAADLPIWWQIPQYLLIGISEIFASIAGLEFAYSAAPKSMQSAIMGL 370

                        170       180       190       200       210       220
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 193788219 155 FFFFSGVGSFVGSGLLALVSIKAIGWMSSHTDFGNINGCYLNYYFFLLAAIQGATLLLFLIISVK 219
Cdd:cd17348  371 FFFFSGVGSFVGSGLLALVSIKEIGWMSSHKDFGNINGCRLNYYFFLLAGIQGVTLLLFLIISVK 435
PTR2 pfam00854
POT family; The POT (proton-dependent oligopeptide transport) family all appear to be proton ...
 1-181 1.63e-39

POT family; The POT (proton-dependent oligopeptide transport) family all appear to be proton dependent transporters.


Pssm-ID: 425909 [Multi-domain]  Cd Length: 392  Bit Score: 140.53  E-value: 1.63e-39
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 193788219    1 MQTTYVLQSLHLripeisNITTTPHTLPAAWLTMFDAVLILLLIPLKDKLVDPILRRHGLLPSSLKRIAVGMFFVMCSAF 80
Cdd:pfam00854 221 MGTSWVLQARTM------NRKIGSFEIPPAQFQAFNAILVLIFIPIFDRLVYPLGRKCTGGLTPLQKMAVGLFLASLAFA 294

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 193788219   81 AAGILESKRLNLVKEKTINQTignvvyhaADLSLWWQVPQYLLIGISEIFASIAGLEFAYSAAPKSMQSAIMGLFFFFSG 160
Cdd:pfam00854 295 IAAIVQLKRNDTGPEFPIAQE--------VPISVLWLIPQYLLIGFGELFISIGGLEFFYKLAPKSMTSLITGLFLLTNA 366

                         170       180
                  ....*....|....*....|....*.
gi 193788219  161 VGSFVGSGLLALV-----SIKAIGWM 181
Cdd:pfam00854 367 LGNFLSGVIATLTakptnVLGAYGWL 392
PTR2 COG3104
Dipeptide/tripeptide permease [Amino acid transport and metabolism];
26-215 5.09e-11

Dipeptide/tripeptide permease [Amino acid transport and metabolism];


Pssm-ID: 442338 [Multi-domain]  Cd Length: 479  Bit Score: 61.75  E-value: 5.09e-11
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 193788219  26 TLPAAWLTMFDAVLILLLIPLKDKLVDPiLRRHGLLPSSLKRIAVGMFFVMCS--AFAAGILESKRLNLVkektinqtig 103
Cdd:COG3104  312 EIPPSWFQSLNPLFIIILAPVFAALWTK-LGKRGKEPSTPTKFALGLLLLGLGflVMALAALLAGAGGKV---------- 380

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 193788219 104 nvvyhaadlSLWWQVPQYLLIGISEIFASIAGLEFAYSAAPKSMQSAIMGLFFFFSGvgsfvgsgllalVSIKAIGWMSS 183
Cdd:COG3104  381 ---------SPLWLVLAYLLLTIGELCLSPVGLSMITKLAPARFAGQMMGLWFLATA------------LGNLLAGLLAG 439

                        170       180       190
                 ....*....|....*....|....*....|..
gi 193788219 184 HTDFGNingcYLNYYFFLLAAIQGATLLLFLI 215
Cdd:COG3104  440 LYAVPG----YTAYFLLLGGIALVAGVVLLLL 467
2A1704 TIGR00926
Peptide:H+ symporter (also transports b-lactam antibiotics, the antitumor agent, bestatin, and ...
 112-151 5.04e-07

Peptide:H+ symporter (also transports b-lactam antibiotics, the antitumor agent, bestatin, and various protease inhibitors); [Transport and binding proteins, Amino acids, peptides and amines]


Pssm-ID: 273343 [Multi-domain]  Cd Length: 654  Bit Score: 50.14  E-value: 5.04e-07
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|
gi 193788219  112 LSLWWQVPQYLLIGISEIFASIAGLEFAYSAAPKSMQSAI 151
Cdd:TIGR00926 563 VSILWQIPQYVILTAGEVLFSVTGLEFSYSQAPPNMKSVL 602
 
Name Accession Description Interval E-value
MFS_SLC15A3_4 cd17348
Solute Carrier family 15 members 3 and 4 of the Major Facilitator Superfamily of transporters; ...
 1-219 8.75e-128

Solute Carrier family 15 members 3 and 4 of the Major Facilitator Superfamily of transporters; Solute carrier family 15 members 3 (SLC15A3) and 4 (SLC15A4) are members of the proton-coupled oligopeptide transporter (POT) family of integral membrane proteins that mediate the cellular uptake of di/tripeptides and peptide-like drugs. They are peptide/histidine transporters (PHTs) that transport free histidine in addition to di/tripeptides. SLC15A4, also called peptide transporter 4 or peptide/histidine transporter 1 (PHT1), is expressed in the human brain, retina, placenta, and immune cells. It is required for Toll-like receptor 7 (TLR7)- and TLR9-mediated type I interferon production in plasmacytoid dendritic cells (pDCs) and is involved in the pathogenesis of lupus-like autoimmunity. SLC15A3, also called osteoclast transporter, peptide transporter 3, or peptide/histidine transporter 2 (PHT2), is expressed in immune tissues including the spleen and thymus. The SLC15A3/SLC15A4 group belongs to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 340906 [Multi-domain]  Cd Length: 435  Bit Score: 368.35  E-value: 8.75e-128
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 193788219   1 MQTTYVLQSLHLRIPEI-----SNITTTPHTLPAAWLTMFDAVLILLLIPLKDKLVDPILRRHGLLPSSLKRIAVGMFFV 75
Cdd:cd17348  211 MQTTYVLQSLHLKIPNIfisniAGITNSSHTFPAAWLTMFDAVLILILIPLKDKLVDPFLKRRGLLPSSLKRIAVGMFFV 290

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 193788219  76 MCSAFAAGILESKRLNLVKEKTINQT-IGNVVYHAADLSLWWQVPQYLLIGISEIFASIAGLEFAYSAAPKSMQSAIMGL 154
Cdd:cd17348  291 MCSAIAAGILESKRLEIVKHNTTIQQqIGNVTYYAADLPIWWQIPQYLLIGISEIFASIAGLEFAYSAAPKSMQSAIMGL 370

                        170       180       190       200       210       220
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 193788219 155 FFFFSGVGSFVGSGLLALVSIKAIGWMSSHTDFGNINGCYLNYYFFLLAAIQGATLLLFLIISVK 219
Cdd:cd17348  371 FFFFSGVGSFVGSGLLALVSIKEIGWMSSHKDFGNINGCRLNYYFFLLAGIQGVTLLLFLIISVK 435
MFS_POT cd06175
Proton-coupled oligopeptide transporter (POT) family of the Major Facilitator Superfamily of ...
 1-219 2.72e-70

Proton-coupled oligopeptide transporter (POT) family of the Major Facilitator Superfamily of transporters; The Proton-coupled oligopeptide transporter (POT) family is present across all major kingdoms of life and is known by a variety of names. It is referred to as the Nitrate transporter/Peptide transporter (NRT1/PTR) family (NPF) in plants, and in addition to POT, it is also known as the Peptide transporter (PepT/PTR) or Solute Carrier 15 (SLC15) family in animals. Members of this family are proton-driven symporters involved in nitrogen acquisition in the form of di- and tripeptides. Plant members transport other nitrogenous ligands including nitrate, the plant hormone auxin, and glucosinolate compounds that are important for seed defense. POT proteins exhibit substrate multispecificity, with one transporter able to recognize as many as 8,400 types of di/tripeptides and certain peptide-like drugs. The POT family belongs to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 340865 [Multi-domain]  Cd Length: 422  Bit Score: 221.16  E-value: 2.72e-70
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 193788219   1 MQTTYVLQSLHLRIPeisnitTTPHTLPAAWLTMFDAVLILLLIPLKDKLVDPILRRHGLLPSSLKRIAVGMFFVMCSAF 80
Cdd:cd06175  221 MTTSYVLQALTLVRS------TGLFTIPPASMQAFNPILLLLLIPVLDKVVYPLIRRYGVGLSPLTKIGLGFFFGSLSMF 294

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 193788219  81 AAGILESKRLNLVKEKtinqtigNVVYHAADLSLWWQVPQYLLIGISEIFASIAGLEFAYSAAPKSMQSAIMGLFFFFSG 160
Cdd:cd06175  295 VAGFLELKRLNTAHAH-------GPVVTTSPLSFWWQIPQYLLLGIGEILVSITGLEFAYREAPKSMKGFIMGLLLLTSG 367

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|....*....
gi 193788219 161 VGSFVGSGLLALVSIKAIGWMsshtDFGNINGCYLNYYFFLLAAIQGATLLLFLIISVK 219
Cdd:cd06175  368 FGSFFGSVLVTLVSKPTDGAH----IADDLNKGVLTNFFFLLAVLAALNFLIFLLVSRK 422
PTR2 pfam00854
POT family; The POT (proton-dependent oligopeptide transport) family all appear to be proton ...
 1-181 1.63e-39

POT family; The POT (proton-dependent oligopeptide transport) family all appear to be proton dependent transporters.


Pssm-ID: 425909 [Multi-domain]  Cd Length: 392  Bit Score: 140.53  E-value: 1.63e-39
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 193788219    1 MQTTYVLQSLHLripeisNITTTPHTLPAAWLTMFDAVLILLLIPLKDKLVDPILRRHGLLPSSLKRIAVGMFFVMCSAF 80
Cdd:pfam00854 221 MGTSWVLQARTM------NRKIGSFEIPPAQFQAFNAILVLIFIPIFDRLVYPLGRKCTGGLTPLQKMAVGLFLASLAFA 294

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 193788219   81 AAGILESKRLNLVKEKTINQTignvvyhaADLSLWWQVPQYLLIGISEIFASIAGLEFAYSAAPKSMQSAIMGLFFFFSG 160
Cdd:pfam00854 295 IAAIVQLKRNDTGPEFPIAQE--------VPISVLWLIPQYLLIGFGELFISIGGLEFFYKLAPKSMTSLITGLFLLTNA 366

                         170       180
                  ....*....|....*....|....*.
gi 193788219  161 VGSFVGSGLLALV-----SIKAIGWM 181
Cdd:pfam00854 367 LGNFLSGVIATLTakptnVLGAYGWL 392
MFS_NPF cd17351
Plant NRT1/PTR family (NPF) of the Major Facilitator Superfamily of transporters; The plant ...
 1-217 4.34e-36

Plant NRT1/PTR family (NPF) of the Major Facilitator Superfamily of transporters; The plant Nitrate transporter/Peptide transporter (NRT1/PTR) family (NPF) is related to the POT (proton-coupled oligopeptide transporter), Peptide transporter (PepT/PTR), or Solute Carrier 15 (SLC15) family in animals. In contrast to related animal and bacterial counterparts, the plant proteins transport a wide variety of substrates including nitrate, peptides, amino acids, dicarboxylates, glucosinolates, as well as the plant hormones indole-3-acetic acid (IAA) and abscisic acid (ABA). A recent study identified eight subfamilies within this family, named NPF1-NPF8. NPF belongs to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 340909 [Multi-domain]  Cd Length: 445  Bit Score: 132.36  E-value: 4.34e-36
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 193788219   1 MQTTYVLQSLHLripeisNITTTPH-TLPAAWLTMFDAVLILLLIPLKDKLVDPILRR-----HGLlpSSLKRIAVGMFF 74
Cdd:cd17351  233 MQTFFVQQGLTM------DRRLGGSfEIPAASLSVFSTLFVLLFIPLYDRVFVPFARRytgnpRGI--TPLQRIGIGLVL 304

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 193788219  75 VMCSAFAAGILESKRLNLVKEKTINQTIGNVVyhaaDLSLWWQVPQYLLIGISEIFASIAGLEFAYSAAPKSMQSAIMGL 154
Cdd:cd17351  305 SILAMAVAALVEVKRLRVARAHGLLDSPTGVV----PMSVFWLLPQYFLLGLAEVFTYIGQLEFFYDEAPDGMRSLGTAL 380

                        170       180       190       200       210       220
                 ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 193788219 155 FFFFSGVGSFVGSGLLALVSIKAIGWMSSHTDFGNINGCYLNYYFFLLAAIQGATLLLFLIIS 217
Cdd:cd17351  381 FLLTIALGNFLSSALVTIVNKITGKGGGPGWLPNNLNKGRLDYFYWLLAVLSAVNFLVFLVCA 443
MFS_NPF8 cd17418
NRT1/PTR family (NPF), subfamily 8 of the Major Facilitator Superfamily of transporters; The ...
 1-220 1.55e-29

NRT1/PTR family (NPF), subfamily 8 of the Major Facilitator Superfamily of transporters; The plant Nitrate transporter/Peptide transporter (NRT1/PTR) family (NPF) is related to the POT (proton-coupled oligopeptide transporter), Peptide transporter (PepT/PTR), or Solute Carrier 15 (SLC15) family in animals. In contrast to related animal and bacterial counterparts, the plant proteins transport a wide variety of substrates including nitrate, peptides, amino acids, dicarboxylates, glucosinolates, as well as the plant hormones indole-3-acetic acid (IAA) and abscisic acid (ABA). A recent study identified eight subfamilies within this family, named NPF1-NPF8. NPF8 contains the Arabidopsis dipeptide transporters AtNPF8.1 (PTR1), AtNPF8.2 (PTR5), and AtNPF8.3 (PTR2), as well as tonoplast-localized transporters AtNPF8.4 (PTR4) and AtNPF8.5 (PTR6). Oryza sativa NRT1 (now called OsNPF8.9) is a low-affinity nitrate transporter. NPF8 belongs to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 340976 [Multi-domain]  Cd Length: 447  Bit Score: 114.49  E-value: 1.55e-29
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 193788219   1 MQTTYVLQSLHLripeisNITTTPHTLPAAWLTMFDAVLILLLIPLKDKLVDPILRRHGLLP---SSLKRIAVGMFFVMC 77
Cdd:cd17418  235 MSTMFVQQGRTM------DRMGGNFEIPPASLSLFDTLSVLIWVPIYDRFIVPLVRKFTGHErgfTQLQRMGIGLFISVL 308

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 193788219  78 SAFAAGILESKRLNLVKEKTINQTIGNVvyhaaDLSLWWQVPQYLLIGISEIFASIAGLEFAYSAAPKSMQSAIMGLFFF 157
Cdd:cd17418  309 SMVAAAIVEIVRLQLARTHNLVESGPPV-----PMSIFWQIPQYFLIGAAEVFTFIGQLEFFYDQSPDAMRSLCSALSLL 383

                        170       180       190       200       210       220
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 193788219 158 FSGVGSFVGSGLLALV-----SIKAIGWMSShtdfgNINGCYLNYYFFLLAAIQGATLLLFLIISVKY 220
Cdd:cd17418  384 TTALGNYLSSLIVTLVtyvttKGGKPGWIPD-----NLNNGHLDYFFWLLASLSFLNILVYLWIAKRY 446
MFS_NPF5 cd17417
NRT1/PTR family (NPF), subfamily 5 of the Major Facilitator Superfamily of transporters; The ...
 26-220 3.99e-28

NRT1/PTR family (NPF), subfamily 5 of the Major Facilitator Superfamily of transporters; The plant Nitrate transporter/Peptide transporter (NRT1/PTR) family (NPF) is related to the POT (proton-coupled oligopeptide transporter), Peptide transporter (PepT/PTR), or Solute Carrier 15 (SLC15) family in animals. In contrast to related animal and bacterial counterparts, the plant proteins transport a wide variety of substrates including nitrate, peptides, amino acids, dicarboxylates, glucosinolates, as well as the plant hormones indole-3-acetic acid (IAA) and abscisic acid (ABA). A recent study identified eight subfamilies within this family, named NPF1-NPF8. NPF5 includes Arabidopsis thaliana PTR3 (AtPTR3, now named AtNPF5.2), which is a wound-induced peptide transporter that is necessary for defense against virulent bacterial pathogens. NPF5 belongs to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 340975 [Multi-domain]  Cd Length: 452  Bit Score: 110.82  E-value: 3.99e-28
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 193788219  26 TLPAAWLTMFDAVLILLLIPLKDKLVDPILRR-----HGLlpSSLKRIAVGMFFVMCSAFAAGILESKRLNLVKEKTINQ 100
Cdd:cd17417  258 KIPPASLQSFITLSILISIPIYDRVFVPIARKitgnpRGI--TLLQRIGIGMFLSILAMVIAALVEMKRLKVAKEHGLIS 335

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 193788219 101 TIGNVvyhaaDLSLWWQVPQYLLIGISEIFASIAGLEFAYSAAPKSMQSAIMGLFFFFSGVGSFVGSGLLALV-----SI 175
Cdd:cd17417  336 PKATV-----PMSIFWLLPQYILLGIADVFTMVGMQEFFYDQVPDEMRSLGTALYLSVFGVGSFLSSFLISIVdkitsSS 410

                        170       180       190       200
                 ....*....|....*....|....*....|....*....|....*
gi 193788219 176 KAIGWMSShtdfgNINGCYLNYYFFLLAAIQGATLLLFLIISVKY 220
Cdd:cd17417  411 GGQSWFNN-----NLNRAHLDYFYWLLAVLSAVNFVLFLFVAKSY 450
MFS_NPF7 cd17419
NRT1/PTR family (NPF), subfamily 7 of the Major Facilitator Superfamily of transporters; The ...
 1-218 1.67e-26

NRT1/PTR family (NPF), subfamily 7 of the Major Facilitator Superfamily of transporters; The plant Nitrate transporter/Peptide transporter (NRT1/PTR) family (NPF) is related to the POT (proton-coupled oligopeptide transporter), Peptide transporter (PepT/PTR), or Solute Carrier 15 (SLC15) family in animals. In contrast to related animal and bacterial counterparts, the plant proteins transport a wide variety of substrates including nitrate, peptides, amino acids, dicarboxylates, glucosinolates, as well as the plant hormones indole-3-acetic acid (IAA) and abscisic acid (ABA). A recent study identified eight subfamilies within this family, named NPF1-NPF8. NPF7 includes the nitrate transporters AtNPF7.2 and AtNPF7.3, as well as the dipeptide transporter OsNPF7.3. AtNPF7.3 is a bidirectional transporter involved in nitrate influx and efflux. NPF7 belongs to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 340977 [Multi-domain]  Cd Length: 447  Bit Score: 106.30  E-value: 1.67e-26
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 193788219   1 MQTTYVLQSlhlripEISNITTTPHTLPAAWLTMFDAVLILLLIPLKDKLVDPILRR---HGLLPSSLKRIAVGMFFVMC 77
Cdd:cd17419  237 MATLFVEQG------AAMDTSFGNFKIPPASMSVFEILSVAVGTVLYRKFLVPLLRKvtgHPKGITELQRMGIGLVIGIL 310

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 193788219  78 SAFAAGILESKRLNLVKEKTINQTIGNVvyhaaDLSLWWQVPQYLLIGISEIFASIAGLEFAYSAAPKSMQSAIMGLFFF 157
Cdd:cd17419  311 AMVVAALVEIQRLKVARPSNLDDPTSPS-----PLSIFWQIPQYVLVGASEVFTYVGQLEFFYDQAPDGLRSLGSALCMA 385

                        170       180       190       200       210       220
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 193788219 158 FSGVGSFVGSGLLALV-----SIKAIGWMSShtdfgNINGCYLNYYFFLLAAIQGATLLLFLIISV 218
Cdd:cd17419  386 SVALGNYLSSLLVTIVnkvttKDGSPGWIPD-----NLNKGHLDRFYWLLAALSGIDFVVFLICAK 446
MFS_SLC15A1_2_like cd17347
Solute carrier family 15 members 1 and 2, and similar Major Facilitator Superfamily ...
 26-220 1.90e-26

Solute carrier family 15 members 1 and 2, and similar Major Facilitator Superfamily transporters; Solute carrier family 15 member 1 (SLC15A1) and SLC15A2 are members of the proton-coupled oligopeptide transporter (POT) family of integral membrane proteins that mediate the cellular uptake of di/tripeptides and peptide-like drugs. They mediate the proton-coupled active transport of a broad range of dipeptides and tripeptides, including zwitterionic, anionic and cationic peptides, as well as a variety of peptide-like drugs such as cefadroxil, enalapril, and valacyclovir. SLC15A1, or peptide transporter 1 (PepT1), is primarily expressed in the brush border membranes of enterocytes of the small intestine and is also known as the intestinal isoform. SLC15A2, or peptide transporter 2 (PepT2), is abundantly expressed in the apical membrane of kidney proximal tubules and is also referred to as the renal isoform. Both proteins transport di/tripeptides, but not tetrapeptides or free amino acids, using the energy generated by an inwardly directed transmembrane proton gradient. The SLC15A1/SLC15A2-like group belongs to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 340905 [Multi-domain]  Cd Length: 427  Bit Score: 105.78  E-value: 1.90e-26
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 193788219  26 TLPAAWLTMFDAVLILLLIPLKDKLVDPILRRHGLLPSSLKRIAVGMFFVMCSAFAAGILESK-RLNLVKEKTINQTIGN 104
Cdd:cd17347  243 TILPDQMQALNPILILILIPLFDYVIYPLLERCGIPLTPLRKMGVGMILAALAFVIAGFLQLRiDGGLPGQPDPGEVTTF 322

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 193788219 105 VVYHAADLSLWWQVPQYLLIGISEIFASIAGLEFAYSAAPKSMQSAIMGLffffsgvgsfvgsgllalvsikaigWMSSh 184
Cdd:cd17347  323 EDIEPNSVSILWQLPQYVVLTAGEVLFSITGLEFAYTQAPKSMKSVVQAA-------------------------WLLT- 376

                        170       180       190       200
                 ....*....|....*....|....*....|....*....|....*
gi 193788219 185 TDFGNINGCYLNY---------YFFLLAAIQGATLLLFLIISVKY 220
Cdd:cd17347  377 VALGNLIVVIVAEliqfssqaaEYFFFAGLMLVVAVVFALLARFY 421
MFS_NPF1_2 cd17416
NRT1/PTR family (NPF), subfamily 1 and 2 of the Major Facilitator Superfamily of transporters; ...
 1-220 1.03e-22

NRT1/PTR family (NPF), subfamily 1 and 2 of the Major Facilitator Superfamily of transporters; The plant Nitrate transporter/Peptide transporter (NRT1/PTR) family (NPF) is related to the POT (proton-coupled oligopeptide transporter), Peptide transporter (PepT/PTR), or Solute Carrier 15 (SLC15) family in animals. In contrast to related animal and bacterial counterparts, the plant proteins transport a wide variety of substrates including nitrate, peptides, amino acids, dicarboxylates, glucosinolates, as well as the plant hormones indole-3-acetic acid (IAA) and abscisic acid (ABA). A recent study identified eight subfamilies within this family, named NPF1-NPF8. NPF1 includes Medicago truncatula LATD/NIP, now named MtNPF1.7, which is a high-affinity nitrate transporter and is involved in nodulation and root architecture. NPF2 members are well-established nitrate and glucosinolate transporters, including Arabidopsis nitrate influx and efflux transporters with varied tissue and developmental specificity. Examples are AtNPF2.7, which is expressed in the cortex of mature roots, and AtNPF2.9, which is expressed in root companion cells where it is involved in phloem loading. NPF1/2 belongs to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 340974 [Multi-domain]  Cd Length: 444  Bit Score: 95.41  E-value: 1.03e-22
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 193788219   1 MQTTY-VLQSLHLRIpeisniTTTPH-TLPAAWLTMFDAVLILLLIPLKDKLVDPILRR---HGLLPSSLKRIAVGMFFV 75
Cdd:cd17416  235 QQMSLtVLQALVMDR------HLGPSfQIPAGSITVFLLITLTIFIPLYDRVLVPLLRKitgRGRGLTLLQRVGIGIVFS 308

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 193788219  76 MCSAFAAGILESKRLNLVkektINQTIGNVvyhaaDLSLWWQVPQYLLIGISEIFASIAGLEFAYSAAPKSMQSAIMGLF 155
Cdd:cd17416  309 ILSMAVAAIVERKRRKTA----LTQGNGTS-----PMSVLWLVPQLVLMGIAEAFHFIGQIEFFYQEFPESMRSTATSLF 379

                        170       180       190       200       210       220       230
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 193788219 156 FFFSGVGSFVGSGLLALV-----SIKAIGWMSShtdfgNINGCYLNYYFFLLAAIQGATLLLFLIISVKY 220
Cdd:cd17416  380 SLGMGVASYLSSALISAVhrttgRGGGGSWLPD-----DINKGRLDYVYWLLAGLGALNLGYFLVCSWRY 444
MFS_NPF6 cd17413
NRT1/PTR family (NPF), subfamily 6 of the Major Facilitator Superfamily of transporters; The ...
 26-217 9.76e-22

NRT1/PTR family (NPF), subfamily 6 of the Major Facilitator Superfamily of transporters; The plant Nitrate transporter 1/Peptide transporter (NRT1/PTR) family (NPF) is related to the POT (proton-coupled oligopeptide transporter), Peptide transporter (PepT/PTR), or Solute Carrier 15 (SLC15) family in animals. In contrast to related animal and bacterial counterparts, the plant proteins transport a wide variety of substrates including nitrate, peptides, amino acids, dicarboxylates, glucosinolates, as well as the plant hormones indole-3-acetic acid (IAA) and abscisic acid (ABA). A recent study identified eight subfamilies within this family, named NPF1-NPF8. NPF6 includes the first identified member of the NRT1/PTR family: Arabidopsis thaliana NRT1.1, now called AtNPF6.3. It is a dual affinity nitrate influx transporter and a nitrate sensor. It also transports auxin and has nitrate efflux activity. NPF6 belongs to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 340971 [Multi-domain]  Cd Length: 457  Bit Score: 92.90  E-value: 9.76e-22
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 193788219  26 TLPAAWLTMFDAVLILLLIPLKDKLVDPILRR-----HGLlpSSLKRIAVGMFFVMCSAFAAGILESKRLNLVKEKTINQ 100
Cdd:cd17413  268 EIPPASMAVFLVATILLTTAVYDRVIVPLCRKltghpQGL--TTLQRIGVGLVFSCLAMAVAALVELKRLRIAHAHGKPV 345

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 193788219 101 TIgnvvyhaADLSLWWQVPQYLLIGISEIFASIAGLEFAYSAAPKSMQSAIMGLFFFFSGVGSFVGSGLLALV-----SI 175
Cdd:cd17413  346 KT-------VPMSVFWLVPQYFLVGAGEAFIYTGQLDFFIRESPKGMKSMSTGLFLSTLSLGFFVSSFLVSIVnrvtgKS 418

                        170       180       190       200
                 ....*....|....*....|....*....|....*....|..
gi 193788219 176 KAIGWMSShtdfgNINGCYLNYYFFLLAAIQGATLLLFLIIS 217
Cdd:cd17413  419 HPHGWLAD-----NLNKGRLDYFYWLLAVLSALNFVIFLVFA 455
MFS_NPF3 cd17415
NRT1/PTR family (NPF), subfamily 3 of the Major Facilitator Superfamily of transporters; The ...
 27-215 1.23e-20

NRT1/PTR family (NPF), subfamily 3 of the Major Facilitator Superfamily of transporters; The plant Nitrate transporter/Peptide transporter (NRT1/PTR) family (NPF) is related to the POT (proton-coupled oligopeptide transporter), Peptide transporter (PepT/PTR), or Solute Carrier 15 (SLC15) family in animals. In contrast to related animal and bacterial counterparts, the plant proteins transport a wide variety of substrates including nitrate, peptides, amino acids, dicarboxylates, glucosinolates, as well as the plant hormones indole-3-acetic acid (IAA) and abscisic acid (ABA). A recent study identified eight subfamilies within this family, named NPF1-NPF8. NPF3 is the smallest NPF subfamily and it includes Cucumis sativus nitrite transporter (CsNitr1), now named CsNPF3.2. It functions as a chloroplast nitrite uptake transporter to remove toxic nitrite from the cytosol. NPF3 belongs to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 340973 [Multi-domain]  Cd Length: 448  Bit Score: 89.81  E-value: 1.23e-20
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 193788219  27 LPAAWLTMFDAVLILLLIPLKDKLVDPILRRHGLLPSS---LKRIAVGMFFVMCSAFAAGILESKRlnlvKEKTINQTIG 103
Cdd:cd17415  258 IPPASMSVFGILSLLTTVPLYDRVLMPVARKFTGNERGitfLQRMGIGLFISILATVVAAFVEVKR----KSVAANHGLL 333

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 193788219 104 NVVYHAADLSLWWQVPQYLLIGISEIFASIAGLEFAYSAAPKSMQSAIMGLFFFFSGVGSFVGSGLLALVSiKAIGWMSS 183
Cdd:cd17415  334 DRPHATVPISVFWLVPQYILHGIAEAFMSIGHLEFFYDQSPESMRSTATALFWSTISLGSYLSTLLVTLVH-KETSRGGR 412

                        170       180       190
                 ....*....|....*....|....*....|..
gi 193788219 184 HTDFGNINGCYLNYYFFLLAAIQGATLLLFLI 215
Cdd:cd17415  413 WWLPNNLNRGRLDYFYWLVAVLQAVNLVYFLI 444
MFS_NPF4 cd17414
NRT1/PTR family (NPF), subfamily 4 of the Major Facilitator Superfamily of transporters; The ...
 1-214 1.18e-18

NRT1/PTR family (NPF), subfamily 4 of the Major Facilitator Superfamily of transporters; The plant Nitrate transporter/Peptide transporter (NRT1/PTR) family (NPF) is related to the POT (proton-coupled oligopeptide transporter), Peptide transporter (PepT/PTR), or Solute Carrier 15 (SLC15) family in animals. In contrast to related animal and bacterial counterparts, the plant proteins transport a wide variety of substrates including nitrate, peptides, amino acids, dicarboxylates, glucosinolates, as well as the plant hormones indole-3-acetic acid (IAA) and abscisic acid (ABA). A recent study identified eight subfamilies within this family, named NPF1-NPF8. Members of the NPF4 subfamily have been shown to transport ABA. NPF4 belongs to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 340972 [Multi-domain]  Cd Length: 456  Bit Score: 84.00  E-value: 1.18e-18
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 193788219   1 MQTTYVLQSLHLRIPEISNITTTPHTLPAawltmFDAVLILLLIPLKDKLVDPILRR---HGLLPSSLKRIAVGMFFVMC 77
Cdd:cd17414  244 LQTFSVQQGSTMNRTKIGSFNIPPASLQA-----IPVIFMLILVPLYDHLLVPFARKitgHESGITHLQRIGIGLVLSTL 318

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 193788219  78 SAFAAGILESKRLNLVKEKTINQTIGNVVyhaadLSLWWQVPQYLLIGISEIFASIAGLEFAYSAAPKSMQSAIMGLFFF 157
Cdd:cd17414  319 SMVVAALVETKRKKVAVENGLLNSKENLP-----ISIFWIALQFLILGISDMFTAVGLLEFFYTESPSSMRSLGTALTWC 393

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|....*...
gi 193788219 158 FSGVGSFVGSGLLALV-SIKAIGWMSSHTDFGNINGCYLNYYFFLLAAIQGATLLLFL 214
Cdd:cd17414  394 SYSFGFYLSSVLVSVVnKVTGSGGHGGWLLGENLNRDRLDLFYWLLCVLSTINFLNYL 451
MFS_PTR2 cd17350
Peptide transporter PTR2 of the Major Facilitator Superfamily of transporters; Fungal peptide ...
 25-154 2.51e-18

Peptide transporter PTR2 of the Major Facilitator Superfamily of transporters; Fungal peptide transporter or permease PTR2 is a member of the proton-coupled oligopeptide transporter (POT) family of integral membrane proteins that mediate the cellular uptake of di/tripeptides and peptide-like drugs. It is a 12-transmembrane domain (TMD) integral membrane protein that translocates di-/tripeptides. As with other POT family proteins, it displays characteristic substrate multispecificity. PTR2 belongs to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 340908 [Multi-domain]  Cd Length: 438  Bit Score: 82.90  E-value: 2.51e-18
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 193788219  25 HTLPAAWLTMFDAVLILLLIPLKDKLVDPILRRHGLLPSSLKRIAVGMFFVMCSAFAAGILESK--RLNLVKEKTINQTI 102
Cdd:cd17350  265 HGVPNDLFQAFDSIALIIFIPILENIIYPLLRKYNIPFKPITRITLGFMFASFSMIYAAVLQAKiyQAGPCYANPTGTCS 344

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|..
gi 193788219 103 GNvvyhaaDLSLWWQVPQYLLIGISEIFASIAGLEFAYSAAPKSMQSAIMGL 154
Cdd:cd17350  345 PN------DIHVWIQIPAYVLIAFSEIFASITGLEYAYTKAPPSMKSFITAL 390
MFS_SLC15A1 cd17412
Solute Carrier family 15 member 1 of the Major Facilitator Superfamily of transporters; Solute ...
 32-151 7.84e-13

Solute Carrier family 15 member 1 of the Major Facilitator Superfamily of transporters; Solute carrier family 15 member 1 (SLC15A1), also called peptide transporter 1 (PepT1), is a member of the proton-coupled oligopeptide transporter (POT) family of integral membrane proteins that mediate the cellular uptake of di/tripeptides and peptide-like drugs. SLC15A1, as well as SLC15A2, mediate the proton-coupled active transport of a broad range of dipeptides and tripeptides, including zwitterionic, anionic and cationic peptides, as well as a variety of peptide-like drugs such as cefadroxil, enalapril, and valacyclovir. SLC15A1 is primarily expressed in the brush border membranes of enterocytes of the small intestine and is also known as the intestinal isoform. It is a high-capacity/low-affinity transporter that drives the transport of di-and tripeptides for metabolic purposes. It's expression is upregulated in the colon during chronic inflammation associated with inflammatory bowel disease. SLC15A1/PepT1 belongs to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 340970 [Multi-domain]  Cd Length: 415  Bit Score: 67.18  E-value: 7.84e-13
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 193788219  32 LTMFDAVLILLLIPLKDKLVDPILRRHGLLPSSLKRIAVGMFFVMCSAFAAGILEskrlnlvkektinqtignvvyhaad 111
Cdd:cd17412  262 MQTVNPILIIIMVPIFDAVIYPLIKKCKINFTPLRKMTVGMFLAALAFVAAALLQ------------------------- 316

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|
gi 193788219 112 LSLWWQVPQYLLIGISEIFASIAGLEFAYSAAPKSMQSAI 151
Cdd:cd17412  317 IQMAWQIPQYFLLTCGEVVFSVTGLEFSYSQAPSNMKSVL 356
PTR2 COG3104
Dipeptide/tripeptide permease [Amino acid transport and metabolism];
26-215 5.09e-11

Dipeptide/tripeptide permease [Amino acid transport and metabolism];


Pssm-ID: 442338 [Multi-domain]  Cd Length: 479  Bit Score: 61.75  E-value: 5.09e-11
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 193788219  26 TLPAAWLTMFDAVLILLLIPLKDKLVDPiLRRHGLLPSSLKRIAVGMFFVMCS--AFAAGILESKRLNLVkektinqtig 103
Cdd:COG3104  312 EIPPSWFQSLNPLFIIILAPVFAALWTK-LGKRGKEPSTPTKFALGLLLLGLGflVMALAALLAGAGGKV---------- 380

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 193788219 104 nvvyhaadlSLWWQVPQYLLIGISEIFASIAGLEFAYSAAPKSMQSAIMGLFFFFSGvgsfvgsgllalVSIKAIGWMSS 183
Cdd:COG3104  381 ---------SPLWLVLAYLLLTIGELCLSPVGLSMITKLAPARFAGQMMGLWFLATA------------LGNLLAGLLAG 439

                        170       180       190
                 ....*....|....*....|....*....|..
gi 193788219 184 HTDFGNingcYLNYYFFLLAAIQGATLLLFLI 215
Cdd:COG3104  440 LYAVPG----YTAYFLLLGGIALVAGVVLLLL 467
MFS_SLC15A2 cd17411
Solute carrier family 15 member 2 of the Major Facilitator Superfamily of transporters; Solute ...
 32-151 1.18e-10

Solute carrier family 15 member 2 of the Major Facilitator Superfamily of transporters; Solute carrier family 15 member 2 (SLC15A2), also called peptide transporter 2 (PepT2), is a member of the proton-coupled oligopeptide transporter (POT) family of integral membrane proteins that mediate the cellular uptake of di/tripeptides and peptide-like drugs. SLC15A2, as well as SLC15A1, mediate the proton-coupled active transport of a broad range of dipeptides and tripeptides, including zwitterionic, anionic and cationic peptides, as well as a variety of peptide-like drugs such as cefadroxil, enalapril, and valacyclovir. SLC15A2 is a high-affinity transporter and is abundantly expressed in the apical membrane of kidney proximal tubules and choroid plexus epithelial cells. It is the major transporter involved in the reclamation of peptide-bound amino acids and peptide-like drugs in the kidney, and is also called the renal isoform. In choroid plexus and the brain, it acts as an efflux transporter and plays a role in regulating peptide/neuropeptide homeostasis. SLC15A2/PepT2 belongs to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 340969 [Multi-domain]  Cd Length: 403  Bit Score: 60.61  E-value: 1.18e-10
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 193788219  32 LTMFDAVLILLLIPLKDKLVDPILRRHGLLPSSLKRIAVGMFFVmCSAFAAGILESkrlnlvkektinqtignvvyhaad 111
Cdd:cd17411  251 MQTLNPLLILVFIPIFDLGIYPLVSKCKINFTSLRKMATGMILA-ALAFAVAAAVE------------------------ 305

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|
gi 193788219 112 LSLWWQVPQYLLIGISEIFASIAGLEFAYSAAPKSMQSAI 151
Cdd:cd17411  306 IKIAWQLPQYVLLSAGEVMFSITGLEFSYSQAPASMKSVL 345
2A1704 TIGR00926
Peptide:H+ symporter (also transports b-lactam antibiotics, the antitumor agent, bestatin, and ...
 112-151 5.04e-07

Peptide:H+ symporter (also transports b-lactam antibiotics, the antitumor agent, bestatin, and various protease inhibitors); [Transport and binding proteins, Amino acids, peptides and amines]


Pssm-ID: 273343 [Multi-domain]  Cd Length: 654  Bit Score: 50.14  E-value: 5.04e-07
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|
gi 193788219  112 LSLWWQVPQYLLIGISEIFASIAGLEFAYSAAPKSMQSAI 151
Cdd:TIGR00926 563 VSILWQIPQYVILTAGEVLFSVTGLEFSYSQAPPNMKSVL 602
MFS_SLC15A5 cd17349
Solute Carrier family 15 member 5 of the Major Facilitator Superfamily of transporters; Solute ...
 5-217 5.08e-05

Solute Carrier family 15 member 5 of the Major Facilitator Superfamily of transporters; Solute carrier family 15 member 5 (SLC15A5) is a member of the proton-coupled oligopeptide transporter (POT) family of integral membrane proteins that mediate the cellular uptake of di/tripeptides and peptide-like drugs. The specific function of SLC15A5 is unknown. SLC15A5 belongs to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 340907 [Multi-domain]  Cd Length: 437  Bit Score: 43.59  E-value: 5.08e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 193788219   5 YVLQSLHlripeiSNITTTPHTLPAAWLTMFDAVLILLLIPLKDKLVDPIL--RRHGLLPSSLkrIAVGMFFVMCSAFAA 82
Cdd:cd17349  236 YYLQTMN------SNLNLGGFLLPIAAMNVISIIPLLILAPFLEYFSSCLFssKRYGPFLSAC--IIAGHLCAALSVMVA 307

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 193788219  83 GILESKRLNLVKektINQTIGNVVYHAADLSLWWQVPQYLLIGISEIFASIAGLEFAYSAAPKSMQSAIMGLFFFFSGVG 162
Cdd:cd17349  308 GFSEIHRKRFPL---VEQTLSGKVLLVSSMPCFQLAPQYVLLGVAEALVTPACSLITFRLVPSSIRGISMHFLTLFNGAG 384

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|....*.
gi 193788219 163 SFVGSGLLALVSIKAIG-WMSSHTDFGNingcyLNYYFFLLAAIQGATLLLFLIIS 217
Cdd:cd17349  385 CFMGALLIQLVYLISEGnWFPNTLNKGN-----LERFFFFLASLMLLNALGFWRIS 435
MFS_DtpA_like cd17346
Dipeptide and tripeptide permease A (DtpA)-like subfamily of the Major Facilitator Superfamily ...
 26-219 1.11e-04

Dipeptide and tripeptide permease A (DtpA)-like subfamily of the Major Facilitator Superfamily of transporters; The DtpA-like subfamily includes four Escherichia coli proteins: dipeptide and tripeptide permeases A (DtpA, TppB or YdgR), B (DtpB or YhiP), C (DtpC or YjdL), and D (DtpD or YbgH). They are proton-dependent permeases that transport di- and tripeptides. DtpA and DtpB display a preference for di- and tripeptides composed of L-amino acids. DtpC shows higher specificity for dipeptides compared to tripeptides, and prefers dipeptides containing a C-terminal lysine residue. The DtpA-like subfamily belongs to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 340904 [Multi-domain]  Cd Length: 399  Bit Score: 42.56  E-value: 1.11e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 193788219  26 TLPAAWLTMFDAVLILLLIPLKDKLVdPILRRHGLLPSSLKRIAVGMFFVMCS----AFAAGILESKRLNlvkektinqt 101
Cdd:cd17346  233 TIPPSWFQSLNPLFIILLAPILAALW-TKLGKRGKDPSIPTKFALGLLLLGLSfllmVLAASFAGGSGKV---------- 301

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 193788219 102 ignvvyhaadlSLWWQVPQYLLIGISEIFASIAGLEFAYSAAPKSMQSAIMGLffffsgvgsfvgsGLLAL-VSIKAIGW 180
Cdd:cd17346  302 -----------SALWLVLSYFLQTLGELCLSPVGLSMITKLAPAKFAGFMMGL-------------WFLASaLGNILAGY 357

                        170       180       190       200
                 ....*....|....*....|....*....|....*....|..
gi 193788219 181 MSSHTDFGNINGCYLN---YYFFLLAAIQGATLLLFLIISVK 219
Cdd:cd17346  358 LAGFYAQPKDPLASALvytDYFGLIGVIALVAGIILLLLAKK 399
2A1704 TIGR00926
Peptide:H+ symporter (also transports b-lactam antibiotics, the antitumor agent, bestatin, and ...
 37-88 8.95e-03

Peptide:H+ symporter (also transports b-lactam antibiotics, the antitumor agent, bestatin, and various protease inhibitors); [Transport and binding proteins, Amino acids, peptides and amines]


Pssm-ID: 273343 [Multi-domain]  Cd Length: 654  Bit Score: 37.05  E-value: 8.95e-03
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|..
gi 193788219   37 AVLILLLIPLKDKLVDPILRRHGLLPSSLKRIAVGMFFVMCSAFAAGILESK 88
Cdd:TIGR00926 290 PLLILIFVPIFDYIVYPAIAKCGTRFTSLRKMAVGGLLAALSFFVAALVQLK 341
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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