calponin homology (CH) domain superfamily; CH domains are actin filament (F-actin) binding motifs, which may be present as a single copy or in tandem repeats (which increase binding affinity). They either function as autonomous actin binding motifs or serve a regulatory function. CH domains are found in cytoskeletal and signal transduction proteins, including actin-binding proteins like spectrin, alpha-actinin, dystrophin, utrophin, and fimbrin, as well as proteins essential for regulation of cell shape (cortexillins), and signaling proteins (Vav).

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 674578327  21 RYDANAEQEVVQWISQIVGETVPLGReNVQRSLKDGKILVRLIDAvyertshLPPAaqSVRrplKPNTMSAPFKQMENIQ 100
Cdd:cd21207    1 KRDPALEAEALDWIEAVTGEKLDDGK-DYEDVLKDGVILCKLINI-------LKPG--SVK---KINTSKMAFKLMENIE 67

                         90       100       110
                 ....*....|....*....|....*....|....*....
gi 674578327 101 IFLDATEAYGVPKTSLFQTVDLYESRNMSQVINTLMQLG 139
Cdd:cd21207   68 NFLTACKGYGVPKTDLFQTVDLYEKKNIPQVTNCLFALG 106

Calponin family repeat;

                          10        20
                  ....*....|....*....|....
gi 674578327  216 SLQMGTNKFASQKGMS-FGSVRHV 238
Cdd:pfam00402   2 GLQMGTNKGASQSGMTaFGAVRHI 25

Calponin family repeat;

                          10        20
                  ....*....|....*....|....*..
gi 674578327  333 LGLQMGpgAKQVATQSGMTSFGAIRHI 359
Cdd:pfam00402   1 IGLQMG--TNKGASQSGMTAFGAVRHI 25

calponin homology (CH) domain found in Drosophila melanogaster muscle-specific protein 20 (dMP20) and similar domains; This subfamily contains Drosophila melanogaster muscle-specific protein 20 (dMP20), Echinococcus granulosus myophilin, Dictyostelium discoideum Rac guanine nucleotide exchange factor B (also called Trix), and similar proteins. dMP20 is present only in the synchronous muscles of D. melanogaster. It may be involved in the system linking the nerve impulse with the contraction or the relaxation process. Trix is involved in the regulation of the late steps of the endocytic pathway. dMP20 contains a single copy of the CH domain, while Trix (triple CH-domain array exchange factor) contains three, two type 3 CH domains which are included in this model, and one type 1 CH domain that is not included in this subfamily, but is part of the superfamily. CH domains are actin filament (F-actin) binding motifs.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 674578327  21 RYDANAEQEVVQWISQIVGETVPLGReNVQRSLKDGKILVRLIDAvyertshLPPAaqSVRrplKPNTMSAPFKQMENIQ 100
Cdd:cd21207    1 KRDPALEAEALDWIEAVTGEKLDDGK-DYEDVLKDGVILCKLINI-------LKPG--SVK---KINTSKMAFKLMENIE 67

                         90       100       110
                 ....*....|....*....|....*....|....*....
gi 674578327 101 IFLDATEAYGVPKTSLFQTVDLYESRNMSQVINTLMQLG 139
Cdd:cd21207   68 NFLTACKGYGVPKTDLFQTVDLYEKKNIPQVTNCLFALG 106

Calponin homology domain; Actin binding domains present in duplicate at the N-termini of spectrin-like proteins (including dystrophin, alpha-actinin). These domains cross-link actin filaments into bundles and networks. A calponin homology domain is predicted in yeasst Cdc24p.

                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 674578327    29 EVVQWISQIVGETVPLGRENVQRSLKDGKILVRLIDAVYertshlpPAAQSVRrplKPNTMSAPFKQMENIQIFLDATEA 108
Cdd:smart00033   2 TLLRWVNSLLAEYDKPPVTNFSSDLKDGVALCALLNSLS-------PGLVDKK---KVAASLSRFKKIENINLALSFAEK 71

                           90       100       110
                   ....*....|....*....|....*....|.
gi 674578327   109 YGvPKTSLFQTVDLYESRNM-SQVINTLMQL 138
Cdd:smart00033  72 LG-GKVVLFEPEDLVEGPKLiLGVIWTLISL 101

Calponin family repeat;

                          10        20
                  ....*....|....*....|....*
gi 674578327  293 ITLQYGTNKFATQSGMTSIGAVRHI 317
Cdd:pfam00402   1 IGLQMGTNKGASQSGMTAFGAVRHI 25

Calponin family repeat;

                          10        20
                  ....*....|....*....|....
gi 674578327  254 ITLQMGTNQCASQKGMS-MGGVRH 276
Cdd:pfam00402   1 IGLQMGTNKGASQSGMTaFGAVRH 24

Calponin family repeat;

                          10        20
                  ....*....|....*....|..
gi 674578327  178 LQSGTNKFASQKGMT-FGGVRH 198
Cdd:pfam00402   3 LQMGTNKGASQSGMTaFGAVRH 24

calponin homology (CH) domain found in Drosophila melanogaster muscle-specific protein 20 (dMP20) and similar domains; This subfamily contains Drosophila melanogaster muscle-specific protein 20 (dMP20), Echinococcus granulosus myophilin, Dictyostelium discoideum Rac guanine nucleotide exchange factor B (also called Trix), and similar proteins. dMP20 is present only in the synchronous muscles of D. melanogaster. It may be involved in the system linking the nerve impulse with the contraction or the relaxation process. Trix is involved in the regulation of the late steps of the endocytic pathway. dMP20 contains a single copy of the CH domain, while Trix (triple CH-domain array exchange factor) contains three, two type 3 CH domains which are included in this model, and one type 1 CH domain that is not included in this subfamily, but is part of the superfamily. CH domains are actin filament (F-actin) binding motifs.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 674578327  21 RYDANAEQEVVQWISQIVGETVPLGReNVQRSLKDGKILVRLIDAvyertshLPPAaqSVRrplKPNTMSAPFKQMENIQ 100
Cdd:cd21207    1 KRDPALEAEALDWIEAVTGEKLDDGK-DYEDVLKDGVILCKLINI-------LKPG--SVK---KINTSKMAFKLMENIE 67

                         90       100       110
                 ....*....|....*....|....*....|....*....
gi 674578327 101 IFLDATEAYGVPKTSLFQTVDLYESRNMSQVINTLMQLG 139
Cdd:cd21207   68 NFLTACKGYGVPKTDLFQTVDLYEKKNIPQVTNCLFALG 106

calponin homology (CH) domain found in transgelin-3; Transgelin-3, also called neuronal protein 22 (NP22), or neuronal protein NP25, may have a role in alcohol-related adaptations and may mediate regulatory signal transduction pathways in neurons. It contains a single copy of the CH domain. CH domains are actin filament (F-actin) binding motifs.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 674578327  23 DANAEQEVVQWISQIVGETV---PLGRENVQRSLKDGKILVRLIDAVYertshlPPAAQSVrrPLKPNTMSApFKQMENI 99
Cdd:cd21281    1 DPDLESRLVDWIIIQCGGDIerpQPGRQNFQKWLMDGTILCRLINSLY------PPGKEPI--KKISETKMA-FKQMEKI 71

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|..
gi 674578327 100 QIFLDATEAYGVPKTSLFQTVDLYESRNMSQVINTLMQLGTE 141
Cdd:cd21281   72 SQFLQAAEAYGVITTDIFQTVDLWEGKDMAAVQRTLMALGSV 113

calponin homology (CH) domain found in transgelin; Transgelin, also called 22 kDa actin-binding protein, protein WS3-10, or smooth muscle protein 22-alpha (SM22-alpha), acts as an actin cross-linking/gelling protein that may be involved in calcium interactions and in regulating contractile properties of the cell. It may also contribute to replicative senescence. Transgelin contains a single copy of the CH domain. CH domains are actin filament (F-actin) binding motifs.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 674578327  23 DANAEQEVVQWISQIVGETV---PLGRENVQRSLKDGKILVRLIDAVYertshlPPAAQSVRRPLKPNTMSapFKQMENI 99
Cdd:cd21279    1 DEELEERLVEWIVVQCGPDVgrpDRGRLGFQVWLKNGVVLSKLVNSLY------PDGSKPVKIPDNPPSMV--FKQMEQV 72

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|.
gi 674578327 100 QIFLDATEAYGVPKTSLFQTVDLYESRNMSQVINTLMQLGT 140
Cdd:cd21279   73 AQFLKAAEDYGVVKTDMFQTVDLYEGKDMAAVQRTLVALGS 113

calponin homology (CH) domain found in Saccharomyces cerevisiae transgelin (SCP1) and similar proteins; The family includes transgelins from Saccharomyces cerevisiae and Schizosaccharomyces pombe, which are also called SCP1 and STG1, respectively. Transgelin, also called calponin homolog 1, has actin-binding and actin-bundling activity. It stabilizes actin filaments against disassembly. Transgelin contains a single copy of the CH domain. CH domains are actin filament (F-actin) binding motifs.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 674578327  26 AEQEVVQWISQIVGETVPLGreNVQRSLKDGKILVRLIDAVYertshlppaAQSVRrplKPNTMSAPFKQMENIQIFLDA 105
Cdd:cd21210    1 AEQEAREWIEEVLGEKLAQG--DLLDALKDGVVLCKLANRIL---------PADIR---KYKESKMPFVQMENISAFLNA 66

                         90       100       110
                 ....*....|....*....|....*....|....
gi 674578327 106 TEAYGVPKTSLFQTVDLYESRNMSQVINTLMQLG 139
Cdd:cd21210   67 ARKLGVPENDLFQTVDLFERKNPAQVLQCLHALS 100

calponin homology (CH) domain found in calponin-3; Calponin-3 (CNN3), also called acidic isoform calponin, is an F-actin-binding protein that is expressed in the brain and has been shown to control dendritic spine morphology, density, and plasticity by regulating actin cytoskeletal reorganization and dynamics. It contains a single copy of the CH domain. CH domains are actin filament (F-actin) binding motifs.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 674578327  21 RYDANAEQEVVQWISQIVGetVPLGrENVQRSLKDGKILVRLIDavyertsHLPPAaqSVRrplKPNTMSAPFKQMENIQ 100
Cdd:cd21284    1 KYDPQKEEELRNWIEEVTG--MSIG-ENFQKGLKDGVILCELIN-------KLQPG--SIR---KINESKLNWHQLENIG 65

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*.
gi 674578327 101 IFLDATEAYGVPKTSLFQTVDLYESRNMSQVINTLMQLGTECQRNG 146
Cdd:cd21284   66 NFIKAIQAYGMKPHDIFEANDLFENGNMTQVQTTLLALAGLAKTKG 111

calponin homology (CH) domain found in the calponin family; Calponin is an actin filament-associated regulatory protein expressed in smooth muscle and many types of non-muscle cells. There are three calponin isoforms, calponin-1, -2, -3. All of them are actin-binding proteins with functions in inhibiting actin-activated myosin ATPase and stabilizing the actin cytoskeleton. Calponin-1 is specifically expressed in smooth muscle cells and plays a role in fine-tuning smooth muscle contractility. Calponin-2 is expressed in both smooth muscle and non-muscle cells and regulates multiple actin cytoskeleton-based functions. Calponin-3 is expressed in the brain and participates in actin cytoskeleton-based activities in embryonic development and myogenesis. Members of this family contain a single copy of the CH domain. CH domains are actin filament (F-actin) binding motifs.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 674578327  23 DANAEQEVVQWISQIVGETVPlgrENVQRSLKDGKILVRLIDAvyertshLPPAaqSVRrplKPNTMSAPFKQMENIQIF 102
Cdd:cd21211    1 DHQKEAELRTWIEGVTGLSIG---PNFQKGLKDGIILCELINK-------LQPG--SVK---KINESMQNWHQLENIGNF 65

                         90       100       110
                 ....*....|....*....|....*....|....*.
gi 674578327 103 LDATEAYGVPKTSLFQTVDLYESRNMSQVINTLMQL 138
Cdd:cd21211   66 IKAIVSYGMKPHDIFEANDLFENGNMTQVQVTLLAL 101

calponin homology (CH) domain found in calponin-1 and similar proteins; Calponin-1 (CNN1), also called basic calponin, or smooth muscle calponin H1, is a thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C, and tropomyosin. Calponin-1 contains a single copy of the CH domain. CH domains are actin filament (F-actin) binding motifs.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 674578327  23 DANAEQEVVQWISQIVGETVPlgrENVQRSLKDGKILVRLIDavyertsHLPPAaqSVRrplKPNTMSAPFKQMENIQIF 102
Cdd:cd21282    1 DPQTEEELRVWIEGVTGRRIG---DNFMDGLKDGVILCELIN-------KLQPG--SVR---KINESTQNWHKLENIGNF 65

                         90       100       110
                 ....*....|....*....|....*....|....*...
gi 674578327 103 LDATEAYGVPKTSLFQTVDLYESRNMSQVINTLMQLGT 140
Cdd:cd21282   66 IKAIMHYGVKPHDIFEANDLFENTNHTQVQSTLIALAS 103

calponin homology (CH) domain found in LIM domain only protein 7 and similar proteins; This family includes LIM domain only protein 7 (LMO-7) and LIM and calponin homology domains-containing protein 1 (LIMCH1), and similar proteins. LMO-7, also called F-box only protein 20, or LOMP, is a transcription regulator for expression of many Emery-Dreifuss muscular dystrophy (EDMD)-relevant genes. It binds to alpha-actinin and AF6/afadin at adherens junctions for epithelial cell-cell adhesion. LIMCH1 acts as an actin stress fiber-associated protein that activates the non-muscle myosin IIa complex by promoting the phosphorylation of its regulatory subunit MRLC/MYL9. It positively regulates actin stress fiber assembly and stabilizes focal adhesions, and therefore negatively regulates cell spreading and cell migration. Members of this family contain a single copy of the CH domain at the N-terminus. CH domains are actin filament (F-actin) binding motifs.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 674578327  26 AEQEVVQWISQIVGETvpLGRENVQRSLKDGKILVRLIDAVYertshlpPaaQSVRrplKPNTMSAPFKQMENIQIFLDA 105
Cdd:cd21208    1 ALKEARTWIEAVTGKK--FPSDDFRESLEDGILLCELINAIK-------P--GSIK---KINRLPTPIAGLDNLNLFLKA 66

                         90       100       110
                 ....*....|....*....|....*....|....*....
gi 674578327 106 TEAYGVPKTSLFQTVDLYESRNmsQVINTLmQLGTECQR 144
Cdd:cd21208   67 CEDLGLKDSQLFDPTDLQDLSN--RRIATH-VRKKEDER 102

calponin homology (CH) domain found in the growth arrest-specific protein 2 family; The growth arrest-specific protein 2 (GAS-2) family includes GAS-2, and GAS-2 like proteins, GAS2L1-3. GAS-2 may play a role in apoptosis by acting as a cell death substrate for caspases. GAS2L1 (also called GAS2-related protein on chromosome 22 or growth arrest-specific protein 2-like 1) and GAS2L2 (also called GAS2-related protein on chromosome 17 or growth arrest-specific protein 2-like 2) may be involved in the cross-linking of microtubules and microfilaments. GAS2L3, also called GAS2-like protein 3, is a cytoskeletal linker protein that may promote and stabilize the formation of the actin and microtubule network. Members of this family contain a single copy of the CH domain at the N-terminal region. CH domains are actin filament (F-actin) binding motifs.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 674578327  28 QEVVQWISQIVGETVplGRENVQRSLKDGKILVRLIDAVYERTSHLPPA------AQSVRRPLKPNTMSAPFKQMENIQI 101
Cdd:cd21204    9 EDLAEWLNDLLGDDL--TPDNFLDELRNGVVLCQLAQKIQEAAEKAREAgkkngpPPSYKLKCNENAKPGSFFARDNVAN 86

                         90       100       110
                 ....*....|....*....|....*....|....*...
gi 674578327 102 FLDATEAYGVPKTSLFQTVDLYESRNMSQVINTLMQLG 139
Cdd:cd21204   87 FLRWCRKLGVDEVLLFESEDLVLHKNPRQVLLCLLELA 124

Calponin family repeat;

                          10        20
                  ....*....|....*....|....*
gi 674578327  293 ITLQYGTNKFATQSGMTSIGAVRHI 317
Cdd:pfam00402   1 IGLQMGTNKGASQSGMTAFGAVRHI 25

calponin homology (CH) domain found in the leucine-rich repeat and calponin homology domain-containing protein family; The leucine-rich repeat and calponin homology domain-containing protein (LRCH) family includes LRCH1-4. LRCH1, also called calponin homology domain-containing protein 1, or neuronal protein 81 (NP81), acts as a negative regulator of GTPase Cdc42 by sequestering Cdc42-guanine exchange factor DOCK8. LRCH2 may play a role in the organization of the cytoskeleton. LRCH3 is part of the DISP complex and may regulate the association of septins with actin and thereby regulate the actin cytoskeleton. LRCH4, also called leucine-rich repeat neuronal protein 4, or leucine-rich neuronal protein, acts as a novel Toll-like receptor (TLR) accessory protein that regulates the innate immune response. Members of this family contain a single copy of the CH domain at the C-terminus. CH domains are actin filament (F-actin) binding motifs.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 674578327  42 VPLgRENVQRSLKDGKILVRLIDAVYERT---SHLPPAAQSVrrplkpntmSAPFKQMENIQIFLDATEAYGVPKTSLFQ 118
Cdd:cd21205   16 VTL-PDDLGEALMDGVVLCHLANHVRPRSvpsIHVPSPAVPK---------LSMAKCRRNVENFLEACRKLGVPEERLCS 85

                         90       100
                 ....*....|....*....|..
gi 674578327 119 TVDLYESRNMSQV---INTLMQ 137
Cdd:cd21205   86 PGDILEEKGLVRVavtVQALLQ 107

calponin homology (CH) domain found in alpha-Pak Interactive eXchange factor; Alpha-Pak Interactive eXchange factor (alpha-PIX), also called PAK-interacting exchange factor alpha, Rho guanine nucleotide exchange factor 6 (ARHGEF6), Rac/Cdc42 guanine nucleotide exchange factor 6, or Cool (Cloned out of Library)-2, activates small GTPases by exchanging bound GDP for free GTP. It acts as a GEF for both Cdc42 and Rac1, and is localized in dendritic spines where it regulates spine morphogenesis. It controls dendritic length and spine density in the hippocampus. Mutations in the ARHGEF6 gene cause X-linked intellectual disability in humans. Alpha-PIX contains a single copy of the CH domain at its N-terminus. CH domains are actin filament (F-actin) binding motifs.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 674578327  25 NAEQEVVQW-ISQIVGE----TVPLGRENVQRSLKDGKILVRLIDAVyertshLPPAAQSVrrPLKPNTMSAPfkqMENI 99
Cdd:cd21265    2 NPEEQTVTWlISLGVLNspkkTISDPEEFLKSSLKDGVVLCKLIERL------LPGSVEKY--CLEPKTEADC---IGNI 70

                         90       100       110
                 ....*....|....*....|....*....|....*.
gi 674578327 100 QIFLDATEAYGVpktSLFQTVDLYESRNMSQVINTL 135
Cdd:cd21265   71 KEFLKGCAALKV---ETFEPDDLYTGENFSKVLSTL 103

Calponin family repeat;

                          10        20
                  ....*....|....*....|....
gi 674578327  254 ITLQMGTNQCASQKGMS-MGGVRH 276
Cdd:pfam00402   1 IGLQMGTNKGASQSGMTaFGAVRH 24

calponin homology (CH) domain found in VAV1 protein; VAV1 is expressed predominantly in the hematopoietic system and it plays an important role in the development and activation of B and T cells. It is activated by tyrosine phosphorylation to function as a guanine nucleotide exchange factor (GEF) for Rho GTPases following cell surface receptor activation, triggering various effects such as cytoskeletal reorganization, transcription regulation, cell cycle progression, and calcium mobilization. It also serves as a scaffold protein and has been shown to interact with Ku70, Socs1, Janus kinase 2, SIAH2, S100B, Abl gene, ZAP-70, SLP76, and Syk, among others. VAV proteins contain several domains that enable their function: N-terminal calponin homology (CH), acidic, RhoGEF (also called Dbl-homologous or DH), Pleckstrin Homology (PH), C1 (zinc finger), SH2, and two SH3 domains. This model corresponds to the CH domain, an actin-binding domain which is present as a single copy in VAV1 protein.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 674578327  51 RSLKDGKILVRLIDavyertsHLPPAAQSVRR-PLKPNtMSApFKQMENIQIFLDA-TEAYGVPKTSLFQTVDLYESRNM 128
Cdd:cd21262   34 QALRDGVLLCQLLN-------NLLPHAVNLREiNLRPQ-MSQ-FLCLKNIRTFLSTcCEKFGLRKSELFEAFDLFDVRDF 104

                         90
                 ....*....|
gi 674578327 129 SQVINTLMQL 138
Cdd:cd21262  105 GKVIDTLSIL 114

calponin homology (CH) domain found in LIM domain only protein 7; LIM domain only protein 7 (LMO-7), also called F-box only protein 20, or LOMP, is a transcription regulator for expression of many Emery-Dreifuss muscular dystrophy (EDMD)-relevant genes. It binds to alpha-actinin and AF6/afadin at adherens junctions for epithelial cell-cell adhesion. It contains a single copy of the CH domain at the N-terminus. CH domains are actin filament (F-actin) binding motifs.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 674578327  26 AEQEVVQWISQIVGETvpLGRENVQRSLKDGKILVRLIDAVYERTSHlppaaqsvrrplKPNTMSAPFKQMENIQIFLDA 105
Cdd:cd21277    1 AFSEAQRWIEAVTGKN--FGNKDFRSALENGVLLCDLINKIKPGIIK------------KINRLSTPIAGLDNINVFLKA 66

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|
gi 674578327 106 TEAYGVPKTSLFQTVDLYE------------SRNMSQVINTLMQLGTECQ 143
Cdd:cd21277   67 CEKLGLKEAQLFHPGDLQDlstrvtvkqeetDRRLKNVLITLYWLGRKAQ 116

calponin homology (CH) domain found in LIM and calponin homology domains-containing protein 1; LIM and calponin homology domains-containing protein 1 (LIMCH1) acts as an actin stress fiber-associated protein that activates the non-muscle myosin IIa complex by promoting the phosphorylation of its regulatory subunit MRLC/MYL9. It positively regulates actin stress fiber assembly and stabilizes focal adhesions, and therefore negatively regulates cell spreading and cell migration. LIMCH1 contains a single copy of the CH domain at the N-terminus. CH domains are actin filament (F-actin) binding motifs.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 674578327  26 AEQEVVQWISQIVGETvpLGRENVQRSLKDGKILVRLIDAVYertshlPPAAQsvrrplKPNTMSAPFKQMENIQIFLDA 105
Cdd:cd21278    1 AFTEAQKWIEQVTGRS--FGDKDFRSGLENGILLCELLNAIK------PGLVK------KINRLPTPIAGLDNITLFLRG 66

                         90       100       110
                 ....*....|....*....|....*....|....*....
gi 674578327 106 TEAYGVPKTSLFQTVDLYESRNMSQVINtlmqlgTECQR 144
Cdd:cd21278   67 CKELGLKESQLFDPGDLQDTSNRVTIKS------SDCSR 99

calponin homology (CH) domain found in Ras GTPase-activating-like protein IQGAP1; IQ motif containing GTPase activating protein 1 (IQGAP1), also called p195, is a homodimeric protein that is widely expressed among vertebrate cell types from early embryogenesis. It plays a crucial role in regulating the dynamics and assembly of the actin cytoskeleton. It belongs to the IQGAP family, which consists of multi-domain proteins having a calponin-homology (CH) domain which binds F-actin, IQGAP-specific repeats, a single WW domain, four IQ motifs that mediate interactions with calmodulin, and a RasGAP related domain that binds active Rho family GTPases. IQGAP1 negatively regulates Ras family GTPases by stimulating their intrinsic GTPase activity. It lacks GAP activity. Both, IQGAP1 and IQGAP2, specifically bind to Cdc42 and Rac1, but not to RhoA. Despite similarities to part of the sequence of RasGAP, neither IQGAP1 nor IQGAP2 interacts with Ras. IQGAP1 contains a single copy of the CH domain at the N-terminus.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 674578327  28 QEVVQWISQIVGETVPLGREnVQRSLKDGKILVRLIDAVYERTSHLPPAAQsvRRPLKPNTMSAPFKQMENIQIFLDATE 107
Cdd:cd21274   12 EEAKRWMEACLGEDLPPTTE-LEEGLRNGVYLAKLGNFFSPKVVSLKKIYD--REQTRYKATGLHFRHTDNVIQWLNAMD 88

                         90       100
                 ....*....|....*....|....*
gi 674578327 108 AYGVPKTSLFQTVDLYESRNMSQVI 132
Cdd:cd21274   89 EIGLPKIFYPETTDIYDRKNMPRCI 113

calponin homology (CH) domain found in growth arrest-specific protein 2; Growth arrest-specific protein 2 (GAS-2) may play a role in apoptosis by acting as a cell death substrate for caspases. It contains a single copy of the CH domain at the N-terminal region. CH domains are actin filament (F-actin) binding motifs.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 674578327  27 EQEVVQWISQIVGETVPlgRENVQRSLKDGKILVRLIDAVYER----TSHLPPAAQSV---RRPLKPNTMSAPFKQMENI 99
Cdd:cd21267    9 KEDLALWLTNLLGKEIT--AESFMEKLDNGALLCQLAETLQEKfkenSADANKPGKSLpvrKIPCRANAPSGSFFARDNT 86

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*....
gi 674578327 100 QIFLDATEAYGVPKTSLFQTVDLYESRNMSQVINTLMQLGTECQRNGFQ 148
Cdd:cd21267   87 ANFLSWCRDVGVGDTCLFESEGLVLHKQPREVCLCLLELGRIAARFGVE 135