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Conserved domains on  [gi|1017634338|emb|CZY64870|]
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type I restriction-modification system%2C M subunit [Enterobacter hormaechei]

Protein Classification

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
N6_Mtase super family cl37510
N-6 DNA Methylase; Restriction-modification (R-M) systems protect a bacterial cell against ...
 163-460 1.59e-98

N-6 DNA Methylase; Restriction-modification (R-M) systems protect a bacterial cell against invasion of foreign DNA by endonucleolytic cleavage of DNA that lacks a site specific modification. The R-M system is a complex containing three polypeptides: M (this family), S (pfam01420), and R. This family consists of N-6 adenine-specific DNA methylase EC:2.1.1.72 from Type I and Type IC restriction systems. These methylases have the same sequence specificity as their corresponding restriction enzymes.


The actual alignment was detected with superfamily member pfam02384:

Pssm-ID: 426749 [Multi-domain]  Cd Length: 310  Bit Score: 299.23  E-value: 1.59e-98
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1017634338 163 YLFEKIASDSAMRGGDFYTPEGISLLFAELLQPKDGDSICDPTCGSGSLLITLGEKIKK-SFKSNNYTLFGQEANRSSWA 241
Cdd:pfam02384  11 YLLRKFAPNAGKSGGEFFTPREVSKLIVELLDPKPGESIYDPACGSGGFLIQAEKFVKEhDGDTNDLSIYGQEKNPTTYR 90

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1017634338 242 LAKMNMIIHNeIN---SRIEWGDVISNPQLldtdNRLIQFDVVASNPPFNIIGWNHDDLIHNDYGRFNLGFPPQSKGDYA 318
Cdd:pfam02384  91 LARMNMILHG-IEyddFHIRHGDTLTSPKF----EDDKKFDVVVANPPFSDKWDANDTLENDPRFRPAYGVAPKSNADLA 165

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1017634338 319 FILHMISTLKsATGRMAILVSHGVLFRGGQEESIRKNLVSEGLLDAVIGLPDRLLLGTGISCAILIFRRDKKHSN--VVF 396
Cdd:pfam02384 166 FLQHIIYYLA-PGGRAAVVLPNGVLFRGGAEGKIRKALVDKDLVETVIALPPNLFYNTSIPTCILFLTKNKAERKgkVLF 244

                         250       260       270       280       290       300
                  ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 1017634338 397 IDASDLAKPVKGRNLITNEIIEKVSECYFERSSISNFSHVASFDEIQENDFNLNISRYVKKHEE 460
Cdd:pfam02384 245 IDASNEFKKEGKLNILTDEHIEKIIDTFGEFKDVDGFSKSATLEEIAANDYNLNVGRYVGTEEE 308
HsdM_N super family cl13579
HsdM N-terminal domain; This domain is found at the N-terminus of the methylase subunit of ...
 14-140 4.20e-09

HsdM N-terminal domain; This domain is found at the N-terminus of the methylase subunit of Type I DNA methyltransferases. This domain family is found in bacteria and archaea, and is typically between 123 and 138 amino acids in length. The family is found in association with pfam02384. Mutations in this region of EcoKI methyltransferase abolish the normally strong preference of this system for methylating hemimethylated substrate. The structure of this domain has been shown to be all alpha-helical.


The actual alignment was detected with superfamily member pfam12161:

Pssm-ID: 463478  Cd Length: 123  Bit Score: 54.61  E-value: 4.20e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1017634338  14 ACDLFRGKVDSSSYKDYIFSMLLLKYISDVKADY--EYDLINEEYSGLLEIVsrVPEVASFYKIYHEakNHGDYIGERID 91
Cdd:pfam12161   8 AADILRGDVDASEYKEYILPLLFLKRLDDVLEEReeEVLELIEPLDSGFGFY--IPSELRWSKLANN--LDNDELGENLN 83

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*....
gi 1017634338  92 DSLKLIdqaidsvcQSRGGYLFESIqFATVNFGARSARDRMLVDLLAIF 140
Cdd:pfam12161  84 DAFPGL--------EELNPDLRGVF-MKDARGIITLKSPDLLKKVIQKF 123
 
Name Accession Description Interval E-value
N6_Mtase pfam02384
N-6 DNA Methylase; Restriction-modification (R-M) systems protect a bacterial cell against ...
 163-460 1.59e-98

N-6 DNA Methylase; Restriction-modification (R-M) systems protect a bacterial cell against invasion of foreign DNA by endonucleolytic cleavage of DNA that lacks a site specific modification. The R-M system is a complex containing three polypeptides: M (this family), S (pfam01420), and R. This family consists of N-6 adenine-specific DNA methylase EC:2.1.1.72 from Type I and Type IC restriction systems. These methylases have the same sequence specificity as their corresponding restriction enzymes.


Pssm-ID: 426749 [Multi-domain]  Cd Length: 310  Bit Score: 299.23  E-value: 1.59e-98
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1017634338 163 YLFEKIASDSAMRGGDFYTPEGISLLFAELLQPKDGDSICDPTCGSGSLLITLGEKIKK-SFKSNNYTLFGQEANRSSWA 241
Cdd:pfam02384  11 YLLRKFAPNAGKSGGEFFTPREVSKLIVELLDPKPGESIYDPACGSGGFLIQAEKFVKEhDGDTNDLSIYGQEKNPTTYR 90

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1017634338 242 LAKMNMIIHNeIN---SRIEWGDVISNPQLldtdNRLIQFDVVASNPPFNIIGWNHDDLIHNDYGRFNLGFPPQSKGDYA 318
Cdd:pfam02384  91 LARMNMILHG-IEyddFHIRHGDTLTSPKF----EDDKKFDVVVANPPFSDKWDANDTLENDPRFRPAYGVAPKSNADLA 165

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1017634338 319 FILHMISTLKsATGRMAILVSHGVLFRGGQEESIRKNLVSEGLLDAVIGLPDRLLLGTGISCAILIFRRDKKHSN--VVF 396
Cdd:pfam02384 166 FLQHIIYYLA-PGGRAAVVLPNGVLFRGGAEGKIRKALVDKDLVETVIALPPNLFYNTSIPTCILFLTKNKAERKgkVLF 244

                         250       260       270       280       290       300
                  ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 1017634338 397 IDASDLAKPVKGRNLITNEIIEKVSECYFERSSISNFSHVASFDEIQENDFNLNISRYVKKHEE 460
Cdd:pfam02384 245 IDASNEFKKEGKLNILTDEHIEKIIDTFGEFKDVDGFSKSATLEEIAANDYNLNVGRYVGTEEE 308
HsdM COG0286
Type I restriction-modification system, DNA methylase subunit [Defense mechanisms];
163-401 1.37e-87

Type I restriction-modification system, DNA methylase subunit [Defense mechanisms];


Pssm-ID: 440055 [Multi-domain]  Cd Length: 243  Bit Score: 268.59  E-value: 1.37e-87
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1017634338 163 YLFEKIASDSAMRGGDFYTPEGISLLFAELLQPKDGDSICDPTCGSGSLLITLGEKIKKSFKS--NNYTLFGQEANRSSW 240
Cdd:COG0286     9 YLLRKFAEESGKKAGEFYTPREVVRLMVELLDPKPGETVYDPACGSGGFLVEAAEYLKEHGGDerKKLSLYGQEINPTTY 88

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1017634338 241 ALAKMNMIIHNEINSRIEWGDVisnpqLLDTDNRLIQFDVVASNPPFNIIgWNHDDLIHNDYGRFNLGFPPQSKGDYAFI 320
Cdd:COG0286    89 RLAKMNLLLHGIGDPNIELGDT-----LSNDGDELEKFDVVLANPPFGGK-WKKEELKDDLLGRFGYGLPPKSNADLLFL 162

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1017634338 321 LHMISTLKSaTGRMAILVSHGVLFRGGqEESIRKNLVSEGLLDAVIGLPDRLLLGTGISCAILIFRRDKKH--SNVVFID 398
Cdd:COG0286   163 QHILSLLKP-GGRAAVVLPDGVLFRGA-EKEIRKKLLENDLLEAIIGLPSNLFYNTGIPTCILFLTKGKPErtGKVLFID 240


                  ...
gi 1017634338 399 ASD 401
Cdd:COG0286   241 ASK 243
hsdM TIGR00497
type I restriction system adenine methylase (hsdM); Function: methylation of specific adenine ...
 7-477 1.09e-62

type I restriction system adenine methylase (hsdM); Function: methylation of specific adenine residues; required for both restriction and modification activities. The ECOR124/3 I enzyme recognizes 5'GAA(N7)RTCG. for E.coli see (J. Mol. Biol. 257: 960-969 (1996)). [DNA metabolism, Restriction/modification]


Pssm-ID: 211578 [Multi-domain]  Cd Length: 501  Bit Score: 212.12  E-value: 1.09e-62
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1017634338   7 LHESLLLACDLFRGKVDSSSYKDYIFSMLLLKYISDVKADY------------EYDLINEEYSGLLE-----IVSR---V 66
Cdd:TIGR00497   5 LEKKIWEIANKLRGSVDGWDFKQYVLGGLFYRFISENLCKYindserrndpsfSYANLTDDYEAIDAlkdaaIASKgffI 84

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1017634338  67 PEVASFYKIYHEAKNHGDY------IGERIDDSlKLIDQAIDSVcqsRGgyLFESIQFATVNFGAR-SARDRMLVDLLAI 139
Cdd:TIGR00497  85 KPSQLFQNVVKSIRENEDLnttlrdIFDDIEKS-ELGDGSKESF---KG--LFKDFNVSEVKLGSTlTIRTEKLKELLTS 158

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1017634338 140 FARPEMNfGYIQGGNEKIKVACRYLFEKIASDSAMRGGDFYTPEGISLLFAEL-LQPKDG-DSICDPTCGSGSLLITLGE 217
Cdd:TIGR00497 159 IDTMELD-EFEKNSIDAFGDAYEFLISMYAQNAGKSGGEFFTPQDISELLARIaIGKKDTvDDVYDMACGSGSLLLQVIK 237

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1017634338 218 KIKKSFKSNNYtlFGQEANRSSWALAKMNMIIHN--EINSRIEWGDVISNPQLLDTDNrliqFDVVASNPPFNI--IGWN 293
Cdd:TIGR00497 238 VLGEKTSLVSY--YGQEINHTTYNLCRMNMILHNidYANFNIINADTLTTKEWENENG----FEVVVSNPPYSIswAGDK 311

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1017634338 294 HDDLIHNDygRFNLG--FPPQSKGDYAFILHMISTLkSATGRMAILVSHGVLFRGGQEESIRKNLVSEGLLDAVIGLPDR 371
Cdd:TIGR00497 312 KSNLVSDV--RFKDAgtLAPNSKADLAFVLHALYVL-GQEGTAAIVCFPGILYREGKEQTIRKYLVDQNFVDAVIQLPSN 388

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1017634338 372 LLLGTGISCAILIFRRDKKHSNVVFIDASDLAKPVKGRNLITNEIIEKVSECYFERSSISNFSHVASFDEIQENDFNLNI 451
Cdd:TIGR00497 389 LFSTTSIATSILVLKKNRKKDPIFFIDGSNEFVREKKNNRLSPKNIEKIVDCFNSKKEEANFAKSVERDKIRESNYDLTV 468

                         490       500
                  ....*....|....*....|....*.
gi 1017634338 452 SRYVKKHEEQAFVDLESLRQQREELL 477
Cdd:TIGR00497 469 GKYVNSEAEKEELDIKVLNHSIDEIV 494
HsdM_N pfam12161
HsdM N-terminal domain; This domain is found at the N-terminus of the methylase subunit of ...
 14-140 4.20e-09

HsdM N-terminal domain; This domain is found at the N-terminus of the methylase subunit of Type I DNA methyltransferases. This domain family is found in bacteria and archaea, and is typically between 123 and 138 amino acids in length. The family is found in association with pfam02384. Mutations in this region of EcoKI methyltransferase abolish the normally strong preference of this system for methylating hemimethylated substrate. The structure of this domain has been shown to be all alpha-helical.


Pssm-ID: 463478  Cd Length: 123  Bit Score: 54.61  E-value: 4.20e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1017634338  14 ACDLFRGKVDSSSYKDYIFSMLLLKYISDVKADY--EYDLINEEYSGLLEIVsrVPEVASFYKIYHEakNHGDYIGERID 91
Cdd:pfam12161   8 AADILRGDVDASEYKEYILPLLFLKRLDDVLEEReeEVLELIEPLDSGFGFY--IPSELRWSKLANN--LDNDELGENLN 83

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*....
gi 1017634338  92 DSLKLIdqaidsvcQSRGGYLFESIqFATVNFGARSARDRMLVDLLAIF 140
Cdd:pfam12161  84 DAFPGL--------EELNPDLRGVF-MKDARGIITLKSPDLLKKVIQKF 123
 
Name Accession Description Interval E-value
N6_Mtase pfam02384
N-6 DNA Methylase; Restriction-modification (R-M) systems protect a bacterial cell against ...
 163-460 1.59e-98

N-6 DNA Methylase; Restriction-modification (R-M) systems protect a bacterial cell against invasion of foreign DNA by endonucleolytic cleavage of DNA that lacks a site specific modification. The R-M system is a complex containing three polypeptides: M (this family), S (pfam01420), and R. This family consists of N-6 adenine-specific DNA methylase EC:2.1.1.72 from Type I and Type IC restriction systems. These methylases have the same sequence specificity as their corresponding restriction enzymes.


Pssm-ID: 426749 [Multi-domain]  Cd Length: 310  Bit Score: 299.23  E-value: 1.59e-98
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1017634338 163 YLFEKIASDSAMRGGDFYTPEGISLLFAELLQPKDGDSICDPTCGSGSLLITLGEKIKK-SFKSNNYTLFGQEANRSSWA 241
Cdd:pfam02384  11 YLLRKFAPNAGKSGGEFFTPREVSKLIVELLDPKPGESIYDPACGSGGFLIQAEKFVKEhDGDTNDLSIYGQEKNPTTYR 90

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1017634338 242 LAKMNMIIHNeIN---SRIEWGDVISNPQLldtdNRLIQFDVVASNPPFNIIGWNHDDLIHNDYGRFNLGFPPQSKGDYA 318
Cdd:pfam02384  91 LARMNMILHG-IEyddFHIRHGDTLTSPKF----EDDKKFDVVVANPPFSDKWDANDTLENDPRFRPAYGVAPKSNADLA 165

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1017634338 319 FILHMISTLKsATGRMAILVSHGVLFRGGQEESIRKNLVSEGLLDAVIGLPDRLLLGTGISCAILIFRRDKKHSN--VVF 396
Cdd:pfam02384 166 FLQHIIYYLA-PGGRAAVVLPNGVLFRGGAEGKIRKALVDKDLVETVIALPPNLFYNTSIPTCILFLTKNKAERKgkVLF 244

                         250       260       270       280       290       300
                  ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 1017634338 397 IDASDLAKPVKGRNLITNEIIEKVSECYFERSSISNFSHVASFDEIQENDFNLNISRYVKKHEE 460
Cdd:pfam02384 245 IDASNEFKKEGKLNILTDEHIEKIIDTFGEFKDVDGFSKSATLEEIAANDYNLNVGRYVGTEEE 308
HsdM COG0286
Type I restriction-modification system, DNA methylase subunit [Defense mechanisms];
163-401 1.37e-87

Type I restriction-modification system, DNA methylase subunit [Defense mechanisms];


Pssm-ID: 440055 [Multi-domain]  Cd Length: 243  Bit Score: 268.59  E-value: 1.37e-87
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1017634338 163 YLFEKIASDSAMRGGDFYTPEGISLLFAELLQPKDGDSICDPTCGSGSLLITLGEKIKKSFKS--NNYTLFGQEANRSSW 240
Cdd:COG0286     9 YLLRKFAEESGKKAGEFYTPREVVRLMVELLDPKPGETVYDPACGSGGFLVEAAEYLKEHGGDerKKLSLYGQEINPTTY 88

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1017634338 241 ALAKMNMIIHNEINSRIEWGDVisnpqLLDTDNRLIQFDVVASNPPFNIIgWNHDDLIHNDYGRFNLGFPPQSKGDYAFI 320
Cdd:COG0286    89 RLAKMNLLLHGIGDPNIELGDT-----LSNDGDELEKFDVVLANPPFGGK-WKKEELKDDLLGRFGYGLPPKSNADLLFL 162

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1017634338 321 LHMISTLKSaTGRMAILVSHGVLFRGGqEESIRKNLVSEGLLDAVIGLPDRLLLGTGISCAILIFRRDKKH--SNVVFID 398
Cdd:COG0286   163 QHILSLLKP-GGRAAVVLPDGVLFRGA-EKEIRKKLLENDLLEAIIGLPSNLFYNTGIPTCILFLTKGKPErtGKVLFID 240


                  ...
gi 1017634338 399 ASD 401
Cdd:COG0286   241 ASK 243
hsdM TIGR00497
type I restriction system adenine methylase (hsdM); Function: methylation of specific adenine ...
 7-477 1.09e-62

type I restriction system adenine methylase (hsdM); Function: methylation of specific adenine residues; required for both restriction and modification activities. The ECOR124/3 I enzyme recognizes 5'GAA(N7)RTCG. for E.coli see (J. Mol. Biol. 257: 960-969 (1996)). [DNA metabolism, Restriction/modification]


Pssm-ID: 211578 [Multi-domain]  Cd Length: 501  Bit Score: 212.12  E-value: 1.09e-62
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1017634338   7 LHESLLLACDLFRGKVDSSSYKDYIFSMLLLKYISDVKADY------------EYDLINEEYSGLLE-----IVSR---V 66
Cdd:TIGR00497   5 LEKKIWEIANKLRGSVDGWDFKQYVLGGLFYRFISENLCKYindserrndpsfSYANLTDDYEAIDAlkdaaIASKgffI 84

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1017634338  67 PEVASFYKIYHEAKNHGDY------IGERIDDSlKLIDQAIDSVcqsRGgyLFESIQFATVNFGAR-SARDRMLVDLLAI 139
Cdd:TIGR00497  85 KPSQLFQNVVKSIRENEDLnttlrdIFDDIEKS-ELGDGSKESF---KG--LFKDFNVSEVKLGSTlTIRTEKLKELLTS 158

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1017634338 140 FARPEMNfGYIQGGNEKIKVACRYLFEKIASDSAMRGGDFYTPEGISLLFAEL-LQPKDG-DSICDPTCGSGSLLITLGE 217
Cdd:TIGR00497 159 IDTMELD-EFEKNSIDAFGDAYEFLISMYAQNAGKSGGEFFTPQDISELLARIaIGKKDTvDDVYDMACGSGSLLLQVIK 237

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1017634338 218 KIKKSFKSNNYtlFGQEANRSSWALAKMNMIIHN--EINSRIEWGDVISNPQLLDTDNrliqFDVVASNPPFNI--IGWN 293
Cdd:TIGR00497 238 VLGEKTSLVSY--YGQEINHTTYNLCRMNMILHNidYANFNIINADTLTTKEWENENG----FEVVVSNPPYSIswAGDK 311

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1017634338 294 HDDLIHNDygRFNLG--FPPQSKGDYAFILHMISTLkSATGRMAILVSHGVLFRGGQEESIRKNLVSEGLLDAVIGLPDR 371
Cdd:TIGR00497 312 KSNLVSDV--RFKDAgtLAPNSKADLAFVLHALYVL-GQEGTAAIVCFPGILYREGKEQTIRKYLVDQNFVDAVIQLPSN 388

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1017634338 372 LLLGTGISCAILIFRRDKKHSNVVFIDASDLAKPVKGRNLITNEIIEKVSECYFERSSISNFSHVASFDEIQENDFNLNI 451
Cdd:TIGR00497 389 LFSTTSIATSILVLKKNRKKDPIFFIDGSNEFVREKKNNRLSPKNIEKIVDCFNSKKEEANFAKSVERDKIRESNYDLTV 468

                         490       500
                  ....*....|....*....|....*.
gi 1017634338 452 SRYVKKHEEQAFVDLESLRQQREELL 477
Cdd:TIGR00497 469 GKYVNSEAEKEELDIKVLNHSIDEIV 494
HsdM_N pfam12161
HsdM N-terminal domain; This domain is found at the N-terminus of the methylase subunit of ...
 14-140 4.20e-09

HsdM N-terminal domain; This domain is found at the N-terminus of the methylase subunit of Type I DNA methyltransferases. This domain family is found in bacteria and archaea, and is typically between 123 and 138 amino acids in length. The family is found in association with pfam02384. Mutations in this region of EcoKI methyltransferase abolish the normally strong preference of this system for methylating hemimethylated substrate. The structure of this domain has been shown to be all alpha-helical.


Pssm-ID: 463478  Cd Length: 123  Bit Score: 54.61  E-value: 4.20e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1017634338  14 ACDLFRGKVDSSSYKDYIFSMLLLKYISDVKADY--EYDLINEEYSGLLEIVsrVPEVASFYKIYHEakNHGDYIGERID 91
Cdd:pfam12161   8 AADILRGDVDASEYKEYILPLLFLKRLDDVLEEReeEVLELIEPLDSGFGFY--IPSELRWSKLANN--LDNDELGENLN 83

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*....
gi 1017634338  92 DSLKLIdqaidsvcQSRGGYLFESIqFATVNFGARSARDRMLVDLLAIF 140
Cdd:pfam12161  84 DAFPGL--------EELNPDLRGVF-MKDARGIITLKSPDLLKKVIQKF 123
YtxK COG0827
Adenine-specific DNA N6-methylase [Replication, recombination and repair];
180-370 1.16e-07

Adenine-specific DNA N6-methylase [Replication, recombination and repair];


Pssm-ID: 440589 [Multi-domain]  Cd Length: 327  Bit Score: 53.41  E-value: 1.16e-07
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1017634338 180 YTPEGISLLFAELLQ---PKDGDSICDPTCGSGSLLITLGEKIKKsfksnNYTLFGQEANRSSWALAKMNM-IIHNEINs 255
Cdd:COG0827    95 MTPDAIGLLIGYLVEkftKKEGLRILDPAVGTGNLLTTVLNQLKK-----KVNAYGVEVDDLLIRLAAVLAnLQGHPVE- 168

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1017634338 256 rIEWGDVIsNPQLLDtdnrliQFDVVASNPPfniIGWNHDDLIHNdygRFNLGFPPQSKgdYA---FILHMISTLKSAtG 332
Cdd:COG0827   169 -LFHQDAL-QPLLID------PVDVVISDLP---VGYYPNDERAK---RFKLKADEGHS--YAhhlFIEQSLNYLKPG-G 231

                         170       180       190
                  ....*....|....*....|....*....|....*...
gi 1017634338 333 RMAILVSHGvLFRGGQEESIRKNLVSEGLLDAVIGLPD 370
Cdd:COG0827   232 YLFFLVPSN-LFESDQAAQLREFLKEKAHIQGLIQLPE 268
UPF0020 pfam01170
Putative RNA methylase family UPF0020; This domain is probably a methylase. It is associated ...
 195-289 5.94e-07

Putative RNA methylase family UPF0020; This domain is probably a methylase. It is associated with the THUMP domain that also occurs with RNA modification domains.


Pssm-ID: 395932 [Multi-domain]  Cd Length: 184  Bit Score: 49.66  E-value: 5.94e-07
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1017634338 195 PKDGDSICDPTCGSGSLLI---TLGEKIKKSFKSNNYT--LFGQEANRSSWALAKMNMiihneinSRIEWGDVISNPQLL 269
Cdd:pfam01170  26 WKPGDPLLDPMCGSGTILIeaaLMGANIAPGKFDARVRapLYGSDIDRRMVQGARLNA-------ENAGVGDLIEFVQAD 98

                          90       100
                  ....*....|....*....|..
gi 1017634338 270 DTDNRLI--QFDVVASNPPFNI 289
Cdd:pfam01170  99 AADLPLLegSVDVIVTNPPYGI 120
RsmC COG2813
16S rRNA G1207 methylase RsmC [Translation, ribosomal structure and biogenesis]; 16S rRNA ...
 186-299 3.04e-04

16S rRNA G1207 methylase RsmC [Translation, ribosomal structure and biogenesis]; 16S rRNA G1207 methylase RsmC is part of the Pathway/BioSystem: 16S rRNA modification


Pssm-ID: 442062 [Multi-domain]  Cd Length: 191  Bit Score: 41.71  E-value: 3.04e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1017634338 186 SLLFAELLQPKDGDSICDPTCGSGSLLITLgekiKKSFKSNNYTLFgqEANRSSWALAKMNMIIHNEINSRIEWGDVISN 265
Cdd:COG2813    38 TRLLLEHLPEPLGGRVLDLGCGYGVIGLAL----AKRNPEARVTLV--DVNARAVELARANAAANGLENVEVLWSDGLSG 111

                          90       100       110
                  ....*....|....*....|....*....|....
gi 1017634338 266 PQlldtDNRliqFDVVASNPPFNIIGWNHDDLIH 299
Cdd:COG2813   112 VP----DGS---FDLILSNPPFHAGRAVDKEVAH 138
Trm11 COG1041
tRNA G10 N-methylase Trm11 [Translation, ribosomal structure and biogenesis]; tRNA G10 ...
 179-290 3.62e-04

tRNA G10 N-methylase Trm11 [Translation, ribosomal structure and biogenesis]; tRNA G10 N-methylase Trm11 is part of the Pathway/BioSystem: tRNA modification


Pssm-ID: 440663 [Multi-domain]  Cd Length: 172  Bit Score: 41.47  E-value: 3.62e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1017634338 179 FYTPEGISLLFAELL----QPKDGDSICDPTCGSGSLLI---TLGekikksfksnnYTLFGQEANRSSWALAKMNMIIHN 251
Cdd:COG1041     4 FFYPGSLDPRLARALvnlaGAKEGDTVLDPFCGTGTILIeagLLG-----------RRVIGSDIDPKMVEGARENLEHYG 72

                          90       100       110
                  ....*....|....*....|....*....|....*....
gi 1017634338 252 EINSRIEWGDvISNPQLLDTdnrliQFDVVASNPPFNII 290
Cdd:COG1041    73 YEDADVIRGD-ARDLPLADE-----SVDAIVTDPPYGRS 105
MTS pfam05175
Methyltransferase small domain; This domain is found in ribosomal RNA small subunit ...
 186-287 2.04e-03

Methyltransferase small domain; This domain is found in ribosomal RNA small subunit methyltransferase C as well as other methyltransferases.


Pssm-ID: 428349 [Multi-domain]  Cd Length: 170  Bit Score: 39.11  E-value: 2.04e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1017634338 186 SLLFAELLQPKDGDSICDPTCGSGSLLITLgekiKKSFKSNNYTLFgqEANRSSWALAKMNMIIHNEINSRIEWGDVISN 265
Cdd:pfam05175  20 SRLLLEHLPKDLSGKVLDLGCGAGVLGAAL----AKESPDAELTMV--DINARALESARENLAANGLENGEVVASDVYSG 93

                          90       100
                  ....*....|....*....|..
gi 1017634338 266 PQLLdtdnrliQFDVVASNPPF 287
Cdd:pfam05175  94 VEDG-------KFDLIISNPPF 108
TrmN6 COG4123
tRNA1(Val) A37 N6-methylase TrmN6 [Translation, ribosomal structure and biogenesis]; tRNA1(Val) ...
 186-287 3.06e-03

tRNA1(Val) A37 N6-methylase TrmN6 [Translation, ribosomal structure and biogenesis]; tRNA1(Val) A37 N6-methylase TrmN6 is part of the Pathway/BioSystem: tRNA modification


Pssm-ID: 443299 [Multi-domain]  Cd Length: 238  Bit Score: 39.36  E-value: 3.06e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1017634338 186 SLLFAELLQPKDGDSICDPTCGSGSLLITLgekikkSFKSNNYTLFGQEANRSSWALAKMNmIIHNEINSRIE--WGDVI 263
Cdd:COG4123    26 AVLLAAFAPVKKGGRVLDLGTGTGVIALML------AQRSPGARITGVEIQPEAAELARRN-VALNGLEDRITviHGDLK 98

                          90       100
                  ....*....|....*....|....
gi 1017634338 264 SNPQLLDTDnrliQFDVVASNPPF 287
Cdd:COG4123    99 EFAAELPPG----SFDLVVSNPPY 118
COG2263 COG2263
Predicted RNA methylase [General function prediction only];
179-287 7.42e-03

Predicted RNA methylase [General function prediction only];


Pssm-ID: 441864 [Multi-domain]  Cd Length: 199  Bit Score: 37.58  E-value: 7.42e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1017634338 179 FYTPEGIS---LLFAELLQPKDGDSICDPTCGSGSLLI---TLGEKikksfksnnyTLFGQEANRSSWALAKMNmiiHNE 252
Cdd:COG2263    24 YPTPAELAaelLHLAYLRGDIEGKTVLDLGCGTGMLAIgaaLLGAK----------KVVGVDIDPEALEIAREN---AER 90

                          90       100       110
                  ....*....|....*....|....*....|....*...
gi 1017634338 253 INSRIEW--GDVisnpqlldTDNRLI-QFDVVASNPPF 287
Cdd:COG2263    91 LGVRVDFirADV--------TRIPLGgSVDTVVMNPPF 120
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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