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Conserved domains on  [gi|285810779|tpg|DAA07563|]
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TPA: aminophospholipid translocase regulatory protein CDC50 [Saccharomyces cerevisiae S288C]

Protein Classification

CDC50/LEM3 family protein( domain architecture ID 10009045)

CDC50/LEM3 family protein similar to Saccharomyces cerevisiae cell division control protein 50, Alkylphosphocholine resistance protein LEM3 and phospholipid translocase non-catalytic subunit CRF1

Graphical summary

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List of domain hits

Name Accession Description Interval E-value
CDC50 COG5035
Cell cycle control protein [Cell division and chromosome partitioning / Transcription / Signal ...
1-369 0e+00

Cell cycle control protein [Cell division and chromosome partitioning / Transcription / Signal transduction mechanisms];


:

Pssm-ID: 227368  Cd Length: 372  Bit Score: 638.84  E-value: 0e+00
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 285810779   1 MVSLFKRGKAPPLTKEGPTSKKPPNTAFRQQRLKAWQPILSPQSVLPLLIFVACIFTPIGIGLIVSATKVQDLTIDYSHC 80
Cdd:COG5035    1 EVFRRKDKKAIVSKPSLLKSRRPPNTAFRQQRLKAWQPILSPRTVLPLLFLIGIVFAPLGGGLLVASSSVQELTIDYTDC 80
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 285810779  81 DTKASTtAFEDIPKKYIKYHFKSKVENKPQWR-LTENENGEQSCELQFEIPNDIKKSIFIYYKITNFYQNHRRYVQSFDT 159
Cdd:COG5035   81 MTLASD-EFSDIPSEYIQFHFKKKVNVLPQWRfSTDEEDDFQKCQIRFTVPSDMKKPVFIYYRLTNFYQNHRRYVKSFDE 159
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 285810779 160 KQILGEPIKKDDLDTSCSPIRSREDKIIYPCGLIANSMFNDTFSQVLSGIDDTEDYNLTNKHISWSIDRHRFKTTKYNAS 239
Cdd:COG5035  160 DQLRGEALKSDDLKSNCKPLSYNEDKIIYPCGLIANSMFNDTFSSLLTGIEDTSNYNLTTKGIAWESDRHRYKKTKYNAS 239
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 285810779 240 DIVPPPNWMKKYPDGYTDENLPDIHTWEEFQVWMRTAAFPKFYKLTLKNESASLPKGKYQMNIELNYPISLFGGTKSFVL 319
Cdd:COG5035  240 DIVPPPNWAKMYPDGYTDDNIPDLSTWEEFQNWMRTAAFPKFSKLAMRNVNDVLPPGTYQLNITLNFPVLEFNGTKSIVL 319
                        330       340       350       360       370
                 ....*....|....*....|....*....|....*....|....*....|
gi 285810779 320 TTNGAIGGRNMSLGVLYLIVAGLCALFGIIFLVKLIFQPRAMGDHTYLNF 369
Cdd:COG5035  320 TTNSVIGGKNYFLGIVYLIVGGICALLGLIFLIKWLFKPRKMADHSYLNW 369
 
Name Accession Description Interval E-value
CDC50 COG5035
Cell cycle control protein [Cell division and chromosome partitioning / Transcription / Signal ...
1-369 0e+00

Cell cycle control protein [Cell division and chromosome partitioning / Transcription / Signal transduction mechanisms];


Pssm-ID: 227368  Cd Length: 372  Bit Score: 638.84  E-value: 0e+00
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 285810779   1 MVSLFKRGKAPPLTKEGPTSKKPPNTAFRQQRLKAWQPILSPQSVLPLLIFVACIFTPIGIGLIVSATKVQDLTIDYSHC 80
Cdd:COG5035    1 EVFRRKDKKAIVSKPSLLKSRRPPNTAFRQQRLKAWQPILSPRTVLPLLFLIGIVFAPLGGGLLVASSSVQELTIDYTDC 80
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 285810779  81 DTKASTtAFEDIPKKYIKYHFKSKVENKPQWR-LTENENGEQSCELQFEIPNDIKKSIFIYYKITNFYQNHRRYVQSFDT 159
Cdd:COG5035   81 MTLASD-EFSDIPSEYIQFHFKKKVNVLPQWRfSTDEEDDFQKCQIRFTVPSDMKKPVFIYYRLTNFYQNHRRYVKSFDE 159
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 285810779 160 KQILGEPIKKDDLDTSCSPIRSREDKIIYPCGLIANSMFNDTFSQVLSGIDDTEDYNLTNKHISWSIDRHRFKTTKYNAS 239
Cdd:COG5035  160 DQLRGEALKSDDLKSNCKPLSYNEDKIIYPCGLIANSMFNDTFSSLLTGIEDTSNYNLTTKGIAWESDRHRYKKTKYNAS 239
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 285810779 240 DIVPPPNWMKKYPDGYTDENLPDIHTWEEFQVWMRTAAFPKFYKLTLKNESASLPKGKYQMNIELNYPISLFGGTKSFVL 319
Cdd:COG5035  240 DIVPPPNWAKMYPDGYTDDNIPDLSTWEEFQNWMRTAAFPKFSKLAMRNVNDVLPPGTYQLNITLNFPVLEFNGTKSIVL 319
                        330       340       350       360       370
                 ....*....|....*....|....*....|....*....|....*....|
gi 285810779 320 TTNGAIGGRNMSLGVLYLIVAGLCALFGIIFLVKLIFQPRAMGDHTYLNF 369
Cdd:COG5035  320 TTNSVIGGKNYFLGIVYLIVGGICALLGLIFLIKWLFKPRKMADHSYLNW 369
CDC50 pfam03381
LEM3 (ligand-effect modulator 3) family / CDC50 family; Members of this family have been ...
63-359 1.80e-141

LEM3 (ligand-effect modulator 3) family / CDC50 family; Members of this family have been predicted to contain transmembrane helices. The family member LEM3 is a ligand-effect modulator, mutation of which increases glucocorticoid receptor activity in response to dexamethasone and also confers increased activity on other intracellular receptors including the progesterone, oestrogen and mineralocorticoid receptors. LEM3 is thought to affect a downstream step in the glucocorticoid receptor pathway. Factors that modulate ligand responsiveness are likely to contribute to the context-specific actions of the glucocorticoid receptor in mammalian cells. The products of genes YNR048w, YNL323w and YCR094w (CDC50) show redundancy of function and are involved in regulation of transcription via CDC39. CDC39 (also known as NOT1) is normally a negative regulator of transcription either by affecting the general RNA polymerase II machinery or by altering chromatin structure. One function of CDC39 is to block activation of the mating response pathway in the absence of pheromone, and mutation causes arrest in G1 by activation of the pathway. It may be that the cold-sensitive arrest in G1 noticed in CDC50 mutants may be due to inactivation of CDC39. The effects of LEM3 on glucocorticoid receptor activity may also be due to effects on transcription via CDC39.


Pssm-ID: 460906  Cd Length: 292  Bit Score: 403.90  E-value: 1.80e-141
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 285810779   63 LIVSATKVQDLTIDYSH-CDTKASTTAFEDIPKkyikyhfkskvenkpqwrlTENENGEQSCELQFEIPNDIKKSIFIYY 141
Cdd:pfam03381   1 LLYASNNVQEIVIDYTDdCATLAPTDNFNPIDY-------------------IQNSNETKTCTIQFTIPKDMKGPVYLYY 61
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 285810779  142 KITNFYQNHRRYVQSFDTKQILGEPIKKDDLDTSCSPIRSR----EDKIIYPCGLIANSMFNDTF-----SQVLSGIDDT 212
Cdd:pfam03381  62 KLTNFYQNHRRYVKSRDDDQLKGKAVSALLSGSSCKPLRYNgardDGKPIYPCGLIANSMFNDTFtlynnSSPLLLNVGT 141
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 285810779  213 EDYNLTNKHISWSIDR-HRFK-TTKYNASDIVPPPNWMKKYPDGYTDENLPDIHTWEEFQVWMRTAAFPKFYKLTLK-NE 289
Cdd:pfam03381 142 STYPLTKKGIAWPSDKeHKFKnPTKYNASDIVPPPNWQKRYPNGYTEDNIPNLSENEDFIVWMRTAALPTFRKLYRRiND 221
                         250       260       270       280       290       300       310
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 285810779  290 SASLPKGKYQMNIELNYPISLFGGTKSFVLTTNGAIGGRNMSLGVLYLIVAGLCALFGIIFLVKLIFQPR 359
Cdd:pfam03381 222 TDGLPAGTYTIDIGYNYPVTSFGGTKSIVLSTTSWIGGKNPFLGIAYLVVGGICLLLGIVFLIIHLIKPR 291
 
Name Accession Description Interval E-value
CDC50 COG5035
Cell cycle control protein [Cell division and chromosome partitioning / Transcription / Signal ...
1-369 0e+00

Cell cycle control protein [Cell division and chromosome partitioning / Transcription / Signal transduction mechanisms];


Pssm-ID: 227368  Cd Length: 372  Bit Score: 638.84  E-value: 0e+00
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 285810779   1 MVSLFKRGKAPPLTKEGPTSKKPPNTAFRQQRLKAWQPILSPQSVLPLLIFVACIFTPIGIGLIVSATKVQDLTIDYSHC 80
Cdd:COG5035    1 EVFRRKDKKAIVSKPSLLKSRRPPNTAFRQQRLKAWQPILSPRTVLPLLFLIGIVFAPLGGGLLVASSSVQELTIDYTDC 80
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 285810779  81 DTKASTtAFEDIPKKYIKYHFKSKVENKPQWR-LTENENGEQSCELQFEIPNDIKKSIFIYYKITNFYQNHRRYVQSFDT 159
Cdd:COG5035   81 MTLASD-EFSDIPSEYIQFHFKKKVNVLPQWRfSTDEEDDFQKCQIRFTVPSDMKKPVFIYYRLTNFYQNHRRYVKSFDE 159
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 285810779 160 KQILGEPIKKDDLDTSCSPIRSREDKIIYPCGLIANSMFNDTFSQVLSGIDDTEDYNLTNKHISWSIDRHRFKTTKYNAS 239
Cdd:COG5035  160 DQLRGEALKSDDLKSNCKPLSYNEDKIIYPCGLIANSMFNDTFSSLLTGIEDTSNYNLTTKGIAWESDRHRYKKTKYNAS 239
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 285810779 240 DIVPPPNWMKKYPDGYTDENLPDIHTWEEFQVWMRTAAFPKFYKLTLKNESASLPKGKYQMNIELNYPISLFGGTKSFVL 319
Cdd:COG5035  240 DIVPPPNWAKMYPDGYTDDNIPDLSTWEEFQNWMRTAAFPKFSKLAMRNVNDVLPPGTYQLNITLNFPVLEFNGTKSIVL 319
                        330       340       350       360       370
                 ....*....|....*....|....*....|....*....|....*....|
gi 285810779 320 TTNGAIGGRNMSLGVLYLIVAGLCALFGIIFLVKLIFQPRAMGDHTYLNF 369
Cdd:COG5035  320 TTNSVIGGKNYFLGIVYLIVGGICALLGLIFLIKWLFKPRKMADHSYLNW 369
CDC50 pfam03381
LEM3 (ligand-effect modulator 3) family / CDC50 family; Members of this family have been ...
63-359 1.80e-141

LEM3 (ligand-effect modulator 3) family / CDC50 family; Members of this family have been predicted to contain transmembrane helices. The family member LEM3 is a ligand-effect modulator, mutation of which increases glucocorticoid receptor activity in response to dexamethasone and also confers increased activity on other intracellular receptors including the progesterone, oestrogen and mineralocorticoid receptors. LEM3 is thought to affect a downstream step in the glucocorticoid receptor pathway. Factors that modulate ligand responsiveness are likely to contribute to the context-specific actions of the glucocorticoid receptor in mammalian cells. The products of genes YNR048w, YNL323w and YCR094w (CDC50) show redundancy of function and are involved in regulation of transcription via CDC39. CDC39 (also known as NOT1) is normally a negative regulator of transcription either by affecting the general RNA polymerase II machinery or by altering chromatin structure. One function of CDC39 is to block activation of the mating response pathway in the absence of pheromone, and mutation causes arrest in G1 by activation of the pathway. It may be that the cold-sensitive arrest in G1 noticed in CDC50 mutants may be due to inactivation of CDC39. The effects of LEM3 on glucocorticoid receptor activity may also be due to effects on transcription via CDC39.


Pssm-ID: 460906  Cd Length: 292  Bit Score: 403.90  E-value: 1.80e-141
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 285810779   63 LIVSATKVQDLTIDYSH-CDTKASTTAFEDIPKkyikyhfkskvenkpqwrlTENENGEQSCELQFEIPNDIKKSIFIYY 141
Cdd:pfam03381   1 LLYASNNVQEIVIDYTDdCATLAPTDNFNPIDY-------------------IQNSNETKTCTIQFTIPKDMKGPVYLYY 61
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 285810779  142 KITNFYQNHRRYVQSFDTKQILGEPIKKDDLDTSCSPIRSR----EDKIIYPCGLIANSMFNDTF-----SQVLSGIDDT 212
Cdd:pfam03381  62 KLTNFYQNHRRYVKSRDDDQLKGKAVSALLSGSSCKPLRYNgardDGKPIYPCGLIANSMFNDTFtlynnSSPLLLNVGT 141
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 285810779  213 EDYNLTNKHISWSIDR-HRFK-TTKYNASDIVPPPNWMKKYPDGYTDENLPDIHTWEEFQVWMRTAAFPKFYKLTLK-NE 289
Cdd:pfam03381 142 STYPLTKKGIAWPSDKeHKFKnPTKYNASDIVPPPNWQKRYPNGYTEDNIPNLSENEDFIVWMRTAALPTFRKLYRRiND 221
                         250       260       270       280       290       300       310
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 285810779  290 SASLPKGKYQMNIELNYPISLFGGTKSFVLTTNGAIGGRNMSLGVLYLIVAGLCALFGIIFLVKLIFQPR 359
Cdd:pfam03381 222 TDGLPAGTYTIDIGYNYPVTSFGGTKSIVLSTTSWIGGKNPFLGIAYLVVGGICLLLGIVFLIIHLIKPR 291
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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