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Conserved domains on  [gi|119567903|gb|EAW47518|]
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ribonuclease T2, isoform CRA_e [Homo sapiens]

Protein Classification

T2 family ribonuclease( domain architecture ID 10099427)

T2 family ribonuclease catalyzes a two-stage endonucleolytic cleavage of RNA to form 3'-nucleotides; similar to Homo sapiens ribonuclease T2, which cleaves preferentially single-stranded RNA molecules between purine and uridine residues, which critically contributes to the supply of catabolic uridine and the generation of purine-2',3'-cyclophosphate-terminated oligoribonucleotides

CATH:  3.90.730.10
EC:  4.6.1.19
Gene Ontology:  GO:0003723|GO:0033897
PubMed:  12109772

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
RNase_T2_euk cd01061
Ribonuclease T2 (RNase T2) is a widespread family of secreted RNases found in every organism ...
 34-169 1.42e-55

Ribonuclease T2 (RNase T2) is a widespread family of secreted RNases found in every organism examined thus far. This family includes RNase Rh, RNase MC1, RNase LE, and self-incompatibility RNases (S-RNases). Plant T2 RNases are expressed during leaf senescence in order to scavenge phosphate from ribonucleotides. They are also expressed in response to wounding or pathogen invasion. S-RNases are thought to prevent self-fertilization by acting as selective cytotoxins of "self" pollen. Generally, RNases have two distinct binding sites: the primary site (B1 site) and the subsite (B2 site), for nucleotides located at the 5'- and 3'- terminal ends of the sessil bond, respectively. This CD includes the eukaryotic RNase T2 family members.


:

Pssm-ID: 238512 [Multi-domain]  Cd Length: 195  Bit Score: 173.29  E-value: 1.42e-55
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 119567903  34 WKKLIMVQHWPETVCEKIQNDCR-DPPDYWTIHGLWPDKSEG-----CNRSWPFNLEEIKDLLPEMRAYWPDVIHSFPNr 107
Cdd:cd01061    1 FDYLQLVLQWPDTYCSTGPCCCRpPPPDSFTIHGLWPDNCSGtypqfCDSSSNFDSILISDLLNELNKYWPDLTGPKNN- 79

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 119567903 108 SRFWKHEWEKHGTCAAqvDALNSQKKYFGRSLELYRELDLNSVLLKLGIKPSINYY--QELKAA 169
Cdd:cd01061   80 QSFWEHEWNKHGTCSS--TLLYNQYDYFDTALKLKDKLDLLKILAKAGIVPSTQTYtlSDIQNA 141
 
Name Accession Description Interval E-value
RNase_T2_euk cd01061
Ribonuclease T2 (RNase T2) is a widespread family of secreted RNases found in every organism ...
 34-169 1.42e-55

Ribonuclease T2 (RNase T2) is a widespread family of secreted RNases found in every organism examined thus far. This family includes RNase Rh, RNase MC1, RNase LE, and self-incompatibility RNases (S-RNases). Plant T2 RNases are expressed during leaf senescence in order to scavenge phosphate from ribonucleotides. They are also expressed in response to wounding or pathogen invasion. S-RNases are thought to prevent self-fertilization by acting as selective cytotoxins of "self" pollen. Generally, RNases have two distinct binding sites: the primary site (B1 site) and the subsite (B2 site), for nucleotides located at the 5'- and 3'- terminal ends of the sessil bond, respectively. This CD includes the eukaryotic RNase T2 family members.


Pssm-ID: 238512 [Multi-domain]  Cd Length: 195  Bit Score: 173.29  E-value: 1.42e-55
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 119567903  34 WKKLIMVQHWPETVCEKIQNDCR-DPPDYWTIHGLWPDKSEG-----CNRSWPFNLEEIKDLLPEMRAYWPDVIHSFPNr 107
Cdd:cd01061    1 FDYLQLVLQWPDTYCSTGPCCCRpPPPDSFTIHGLWPDNCSGtypqfCDSSSNFDSILISDLLNELNKYWPDLTGPKNN- 79

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 119567903 108 SRFWKHEWEKHGTCAAqvDALNSQKKYFGRSLELYRELDLNSVLLKLGIKPSINYY--QELKAA 169
Cdd:cd01061   80 QSFWEHEWNKHGTCSS--TLLYNQYDYFDTALKLKDKLDLLKILAKAGIVPSTQTYtlSDIQNA 141
Ribonuclease_T2 pfam00445
Ribonuclease T2 family;
34-169 3.40e-55

Ribonuclease T2 family;


Pssm-ID: 459812  Cd Length: 181  Bit Score: 172.15  E-value: 3.40e-55
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 119567903   34 WKKLIMVQHWPETVCEKIQNDCR-DPPDYWTIHGLWPDKSEG------CNRSWPFNLEEIKDLLPEMRAYWPDVIHSfpN 106
Cdd:pfam00445   1 FDFLLLTQQWPGTYCDTKPSCCGpDSGADFTIHGLWPDNDGGggypqfCDRSRPFDPSEISDLLNDLNKYWPSLKSG--N 78

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 119567903  107 RSRFWKHEWEKHGTCAAQVdaLNSQKKYFGRSLELYRELDLNSVLLKLGIKPSINYY---QELKAA 169
Cdd:pfam00445  79 GESFWKHEWEKHGTCASTS--LDDEHDYFNAALKLRKKLNLLSALASAGIVPSDTKTytlSDIKDA 142
RnaI COG3719
Ribonuclease I [Translation, ribosomal structure and biogenesis];
55-169 2.48e-11

Ribonuclease I [Translation, ribosomal structure and biogenesis];


Pssm-ID: 442933  Cd Length: 222  Bit Score: 59.60  E-value: 2.48e-11
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 119567903  55 CRDPPDY-WTIHGLWPdkseGCNRSWPFN--LEEIkDLLPEMRAYWPDVihsFPNRSRFWkHEWEKHGTCAAQvdalnSQ 131
Cdd:COG3719   61 CRAGRAYgFVLHGLWP----QYERGWPSYcgTPEP-ALSRATRAALADV---MPSAGLAR-HEWKKHGTCSGL-----SP 126

                         90       100       110
                 ....*....|....*....|....*....|....*...
gi 119567903 132 KKYFGRSLELYRELDLNSVLLKLgikpsiNYYQELKAA 169
Cdd:COG3719  127 DDYFALARRLREAVNIPAVGRAL------NIGKTVTAA 158
 
Name Accession Description Interval E-value
RNase_T2_euk cd01061
Ribonuclease T2 (RNase T2) is a widespread family of secreted RNases found in every organism ...
 34-169 1.42e-55

Ribonuclease T2 (RNase T2) is a widespread family of secreted RNases found in every organism examined thus far. This family includes RNase Rh, RNase MC1, RNase LE, and self-incompatibility RNases (S-RNases). Plant T2 RNases are expressed during leaf senescence in order to scavenge phosphate from ribonucleotides. They are also expressed in response to wounding or pathogen invasion. S-RNases are thought to prevent self-fertilization by acting as selective cytotoxins of "self" pollen. Generally, RNases have two distinct binding sites: the primary site (B1 site) and the subsite (B2 site), for nucleotides located at the 5'- and 3'- terminal ends of the sessil bond, respectively. This CD includes the eukaryotic RNase T2 family members.


Pssm-ID: 238512 [Multi-domain]  Cd Length: 195  Bit Score: 173.29  E-value: 1.42e-55
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 119567903  34 WKKLIMVQHWPETVCEKIQNDCR-DPPDYWTIHGLWPDKSEG-----CNRSWPFNLEEIKDLLPEMRAYWPDVIHSFPNr 107
Cdd:cd01061    1 FDYLQLVLQWPDTYCSTGPCCCRpPPPDSFTIHGLWPDNCSGtypqfCDSSSNFDSILISDLLNELNKYWPDLTGPKNN- 79

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 119567903 108 SRFWKHEWEKHGTCAAqvDALNSQKKYFGRSLELYRELDLNSVLLKLGIKPSINYY--QELKAA 169
Cdd:cd01061   80 QSFWEHEWNKHGTCSS--TLLYNQYDYFDTALKLKDKLDLLKILAKAGIVPSTQTYtlSDIQNA 141
Ribonuclease_T2 pfam00445
Ribonuclease T2 family;
34-169 3.40e-55

Ribonuclease T2 family;


Pssm-ID: 459812  Cd Length: 181  Bit Score: 172.15  E-value: 3.40e-55
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 119567903   34 WKKLIMVQHWPETVCEKIQNDCR-DPPDYWTIHGLWPDKSEG------CNRSWPFNLEEIKDLLPEMRAYWPDVIHSfpN 106
Cdd:pfam00445   1 FDFLLLTQQWPGTYCDTKPSCCGpDSGADFTIHGLWPDNDGGggypqfCDRSRPFDPSEISDLLNDLNKYWPSLKSG--N 78

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 119567903  107 RSRFWKHEWEKHGTCAAQVdaLNSQKKYFGRSLELYRELDLNSVLLKLGIKPSINYY---QELKAA 169
Cdd:pfam00445  79 GESFWKHEWEKHGTCASTS--LDDEHDYFNAALKLRKKLNLLSALASAGIVPSDTKTytlSDIKDA 142
RNase_T2 cd00374
Ribonuclease T2 (RNase T2) is a widespread family of secreted RNases found in every organism ...
 34-159 3.85e-47

Ribonuclease T2 (RNase T2) is a widespread family of secreted RNases found in every organism examined thus far. This family includes RNase Rh, RNase MC1, RNase LE, and self-incompatibility RNases (S-RNases). Plant T2 RNases are expressed during leaf senescence in order to scavenge phosphate from ribonucleotides. They are also expressed in response to wounding or pathogen invasion. S-RNases are thought to prevent self-fertilization by acting as selective cytotoxins of "self" pollen.


Pssm-ID: 238220  Cd Length: 195  Bit Score: 151.84  E-value: 3.85e-47
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 119567903  34 WKKLIMVQHWPETVCEKIQNDC--RDPPDYWTIHGLWPDKSEG-----CNRSWPFNLEEIKDLLPEMRAYWPDVIHsfPN 106
Cdd:cd00374    1 FDYYVLVLQWPPTFCATGPCKCcgTPPPDSFTIHGLWPDNCDGtypqfCDSSSFFDKSKDSDLLDELNKYWPDLMP--GK 78

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|...
gi 119567903 107 RSRFWKHEWEKHGTCAAQvdaLNSQKKYFGRSLELYRELDLNSVLLKLGIKPS 159
Cdd:cd00374   79 DSSFWKHEWNKHGTCSGT---LLDQDDYFRTALKLLDKLDLLSILAKAGIKPS 128
RnaI COG3719
Ribonuclease I [Translation, ribosomal structure and biogenesis];
55-169 2.48e-11

Ribonuclease I [Translation, ribosomal structure and biogenesis];


Pssm-ID: 442933  Cd Length: 222  Bit Score: 59.60  E-value: 2.48e-11
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 119567903  55 CRDPPDY-WTIHGLWPdkseGCNRSWPFN--LEEIkDLLPEMRAYWPDVihsFPNRSRFWkHEWEKHGTCAAQvdalnSQ 131
Cdd:COG3719   61 CRAGRAYgFVLHGLWP----QYERGWPSYcgTPEP-ALSRATRAALADV---MPSAGLAR-HEWKKHGTCSGL-----SP 126

                         90       100       110
                 ....*....|....*....|....*....|....*...
gi 119567903 132 KKYFGRSLELYRELDLNSVLLKLgikpsiNYYQELKAA 169
Cdd:COG3719  127 DDYFALARRLREAVNIPAVGRAL------NIGKTVTAA 158
RNase_T2_prok cd01062
Ribonuclease T2 (RNase T2) is a widespread family of secreted RNases found in every organism ...
 48-151 1.36e-10

Ribonuclease T2 (RNase T2) is a widespread family of secreted RNases found in every organism examined thus far. This family includes RNase Rh, RNase MC1, RNase LE, and self-incompatibility RNases (S-RNases). Plant T2 RNases are expressed during leaf senescence in order to scavenge phosphate from ribonucleotides. They are also expressed in response to wounding or pathogen invasion. S-RNases are thought to prevent self-fertilization by acting as selective cytotoxins of "self" pollen. Generally, RNases have two distinct binding sites: the primary site (B1 site) and the subsite (B2 site), for nucleotides located at the 5'- and 3'- terminal ends of the sessil bond, respectively. This CD includes the prokaryotic RNase T2 family members.


Pssm-ID: 238513  Cd Length: 184  Bit Score: 57.00  E-value: 1.36e-10
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 119567903  48 CEKIQNDCRDPPDYWTIHGLWPDK-----SEGCNRSWPFNL--EEIKDLLPEMRAywPDVIhsfpnrsrfwKHEWEKHGT 120
Cdd:cd01062   21 RPECATCGTLDAYGFTLHGLWPQKpkggwPEYCGVTSEPPLseETRSRLLDVMPA--SGLI----------RHEWRKHGT 88

                         90       100       110
                 ....*....|....*....|....*....|.
gi 119567903 121 CAAQvdalnSQKKYFGRSLELYRELDLNSVL 151
Cdd:cd01062   89 CSGL-----DPDAYFAKARNLREALKIPPEL 114
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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