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Conserved domains on  [gi|149043899|gb|EDL97350|]
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sirtuin 1 ((silent mating type information regulation 2, homolog) 1 (S. cerevisiae) (predicted), isoform CRA_a [Rattus norvegicus]

Protein Classification

SIR2 family protein( domain architecture ID 1222)

SIR2 family protein similar to NAD-dependent deacetylase that catalyzes NAD+-dependent protein/histone deacetylation, where the acetyl group from the lysine epsilon-amino group is transferred to the ADP-ribose moiety of NAD+, producing nicotinamide and the novel metabolite O-acetyl-ADP-ribose

PubMed:  7498786

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
SIR2 super family cl00195
SIR2 superfamily of proteins includes silent information regulator 2 (Sir2) enzymes which ...
 62-122 1.08e-32

SIR2 superfamily of proteins includes silent information regulator 2 (Sir2) enzymes which catalyze NAD+-dependent protein/histone deacetylation, where the acetyl group from the lysine epsilon-amino group is transferred to the ADP-ribose moiety of NAD+, producing nicotinamide and the novel metabolite O-acetyl-ADP-ribose. Sir2 proteins, also known as sirtuins, are found in all eukaryotes and many archaea and prokaryotes and have been shown to regulate gene silencing, DNA repair, metabolic enzymes, and life span. The most-studied function, gene silencing, involves the inactivation of chromosome domains containing key regulatory genes by packaging them into a specialized chromatin structure that is inaccessible to DNA-binding proteins. The oligomerization state of Sir2 appears to be organism-dependent, sometimes occurring as a monomer and sometimes as a multimer. Also included in this superfamily is a group of uncharacterized Sir2-like proteins which lack certain key catalytic residues and conserved zinc binding cysteines.


The actual alignment was detected with superfamily member cd01408:

Pssm-ID: 444738  Cd Length: 235  Bit Score: 114.65  E-value: 1.08e-32
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 149043899  62 KKIIVLTGAGVSVSCGIPDFRSRD-GIYARLAVDFpdLPDPQAMFDIEYFRKDPRPFFKFAK 122
Cdd:cd01408    1 KKIVVLVGAGISTSAGIPDFRSPGtGLYANLARYN--LPYPEAMFDISYFRKNPRPFYALAK 60
 
Name Accession Description Interval E-value
SIRT1 cd01408
SIRT1: Eukaryotic group (class1) which includes human sirtuins SIRT1-3 and yeast Hst1-4; and ...
 62-122 1.08e-32

SIRT1: Eukaryotic group (class1) which includes human sirtuins SIRT1-3 and yeast Hst1-4; and are members of the SIR2 family of proteins, silent information regulator 2 (Sir2) enzymes which catalyze NAD+-dependent protein/histone deacetylation. Sir2 proteins have been shown to regulate gene silencing, DNA repair, and life span. The most-studied function, gene silencing, involves the inactivation of chromosome domains containing key regulatory genes by packaging them into a specialized chromatin structure that is inaccessible to DNA-binding proteins. The nuclear SIRT1 has been shown to target the p53 tumor suppressor protein for deacetylation to suppress DNA damage, and the cytoplasmic SIRT2 homolog has been shown to target alpha-tubulin for deacetylation for the maintenance of cell integrity.


Pssm-ID: 238699  Cd Length: 235  Bit Score: 114.65  E-value: 1.08e-32
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 149043899  62 KKIIVLTGAGVSVSCGIPDFRSRD-GIYARLAVDFpdLPDPQAMFDIEYFRKDPRPFFKFAK 122
Cdd:cd01408    1 KKIVVLVGAGISTSAGIPDFRSPGtGLYANLARYN--LPYPEAMFDISYFRKNPRPFYALAK 60
SIR2 pfam02146
Sir2 family; This region is characteriztic of Silent information regulator 2 (Sir2) proteins, ...
 69-124 2.33e-22

Sir2 family; This region is characteriztic of Silent information regulator 2 (Sir2) proteins, or sirtuins. These are protein deacetylases that depend on nicotine adenine dinucleotide (NAD). They are found in many subcellular locations, including the nucleus, cytoplasm and mitochondria. Eukaryotic forms play in important role in the regulation of transcriptional repression. Moreover, they are involved in microtubule organization and DNA damage repair processes.i


Pssm-ID: 426621  Cd Length: 179  Bit Score: 86.54  E-value: 2.33e-22
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*.
gi 149043899   69 GAGVSVSCGIPDFRSRDGIYARLAVDFpdLPDPQAMFDIEYFRKDPRPFFKFAKKK 124
Cdd:pfam02146   1 GAGISTESGIPDFRSDDGLYAKLAPEE--LASPEAFFSNPELVWDPEPFYNIAREL 54
SIR2 COG0846
NAD-dependent protein deacetylase, SIR2 family [Posttranslational modification, protein ...
 49-120 1.19e-21

NAD-dependent protein deacetylase, SIR2 family [Posttranslational modification, protein turnover, chaperones];


Pssm-ID: 440607  Cd Length: 243  Bit Score: 85.98  E-value: 1.19e-21
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 149043899  49 NTIEDAVKLLQECKKIIVLTGAGVSVSCGIPDFRSRDGIYARLavdfpdlpDPQAMFDIEYFRKDPRPFFKF 120
Cdd:COG0846    2 TKIERLAELLREAKRIVVLTGAGISAESGIPDFRGPDGLWEKY--------DPEEVASPEAFRRDPELVWAF 65
PRK00481 PRK00481
NAD-dependent deacetylase; Provisional
 49-126 8.25e-21

NAD-dependent deacetylase; Provisional


Pssm-ID: 234777  Cd Length: 242  Bit Score: 83.69  E-value: 8.25e-21
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 149043899  49 NTIEDAVKLLQECKKIIVLTGAGVSVSCGIPDFRSRDGIYARLavdfpdlpDPQAMFDIEYFRKDPRPFFKFAKKKQH 126
Cdd:PRK00481   1 MRIEELAEILDKAKRIVVLTGAGISAESGIPDFRSANGLWEEH--------RPEDVASPEGFARDPELVWKFYNERRR 70
 
Name Accession Description Interval E-value
SIRT1 cd01408
SIRT1: Eukaryotic group (class1) which includes human sirtuins SIRT1-3 and yeast Hst1-4; and ...
 62-122 1.08e-32

SIRT1: Eukaryotic group (class1) which includes human sirtuins SIRT1-3 and yeast Hst1-4; and are members of the SIR2 family of proteins, silent information regulator 2 (Sir2) enzymes which catalyze NAD+-dependent protein/histone deacetylation. Sir2 proteins have been shown to regulate gene silencing, DNA repair, and life span. The most-studied function, gene silencing, involves the inactivation of chromosome domains containing key regulatory genes by packaging them into a specialized chromatin structure that is inaccessible to DNA-binding proteins. The nuclear SIRT1 has been shown to target the p53 tumor suppressor protein for deacetylation to suppress DNA damage, and the cytoplasmic SIRT2 homolog has been shown to target alpha-tubulin for deacetylation for the maintenance of cell integrity.


Pssm-ID: 238699  Cd Length: 235  Bit Score: 114.65  E-value: 1.08e-32
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 149043899  62 KKIIVLTGAGVSVSCGIPDFRSRD-GIYARLAVDFpdLPDPQAMFDIEYFRKDPRPFFKFAK 122
Cdd:cd01408    1 KKIVVLVGAGISTSAGIPDFRSPGtGLYANLARYN--LPYPEAMFDISYFRKNPRPFYALAK 60
SIR2-fam cd01407
SIR2 family of proteins includes silent information regulator 2 (Sir2) enzymes which catalyze ...
 62-123 1.21e-23

SIR2 family of proteins includes silent information regulator 2 (Sir2) enzymes which catalyze NAD+-dependent protein/histone deacetylation, where the acetyl group from the lysine epsilon-amino group is transferred to the ADP-ribose moiety of NAD+, producing nicotinamide and the novel metabolite O-acetyl-ADP-ribose. Sir2 proteins, also known as sirtuins, are found in all eukaryotes and many archaea and prokaryotes and have been shown to regulate gene silencing, DNA repair, metabolic enzymes, and life span. The most-studied function, gene silencing, involves the inactivation of chromosome domains containing key regulatory genes by packaging them into a specialized chromatin structure that is inaccessible to DNA-binding proteins. The oligomerization state of Sir2 appears to be organism-dependent, sometimes occurring as a monomer and sometimes as a multimer.


Pssm-ID: 238698  Cd Length: 218  Bit Score: 90.71  E-value: 1.21e-23
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 149043899  62 KKIIVLTGAGVSVSCGIPDFRSRDGIYARLAVDFPdlpdpqaMFDIEYFRKDPRPFFKFAKK 123
Cdd:cd01407    1 KRIVVLTGAGISTESGIPDFRSPGGLWARLDPEEL-------AFSPEAFRRDPELFWGFYRE 55
SIR2 pfam02146
Sir2 family; This region is characteriztic of Silent information regulator 2 (Sir2) proteins, ...
 69-124 2.33e-22

Sir2 family; This region is characteriztic of Silent information regulator 2 (Sir2) proteins, or sirtuins. These are protein deacetylases that depend on nicotine adenine dinucleotide (NAD). They are found in many subcellular locations, including the nucleus, cytoplasm and mitochondria. Eukaryotic forms play in important role in the regulation of transcriptional repression. Moreover, they are involved in microtubule organization and DNA damage repair processes.i


Pssm-ID: 426621  Cd Length: 179  Bit Score: 86.54  E-value: 2.33e-22
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*.
gi 149043899   69 GAGVSVSCGIPDFRSRDGIYARLAVDFpdLPDPQAMFDIEYFRKDPRPFFKFAKKK 124
Cdd:pfam02146   1 GAGISTESGIPDFRSDDGLYAKLAPEE--LASPEAFFSNPELVWDPEPFYNIAREL 54
SIR2 COG0846
NAD-dependent protein deacetylase, SIR2 family [Posttranslational modification, protein ...
 49-120 1.19e-21

NAD-dependent protein deacetylase, SIR2 family [Posttranslational modification, protein turnover, chaperones];


Pssm-ID: 440607  Cd Length: 243  Bit Score: 85.98  E-value: 1.19e-21
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 149043899  49 NTIEDAVKLLQECKKIIVLTGAGVSVSCGIPDFRSRDGIYARLavdfpdlpDPQAMFDIEYFRKDPRPFFKF 120
Cdd:COG0846    2 TKIERLAELLREAKRIVVLTGAGISAESGIPDFRGPDGLWEKY--------DPEEVASPEAFRRDPELVWAF 65
PRK00481 PRK00481
NAD-dependent deacetylase; Provisional
 49-126 8.25e-21

NAD-dependent deacetylase; Provisional


Pssm-ID: 234777  Cd Length: 242  Bit Score: 83.69  E-value: 8.25e-21
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 149043899  49 NTIEDAVKLLQECKKIIVLTGAGVSVSCGIPDFRSRDGIYARLavdfpdlpDPQAMFDIEYFRKDPRPFFKFAKKKQH 126
Cdd:PRK00481   1 MRIEELAEILDKAKRIVVLTGAGISAESGIPDFRSANGLWEEH--------RPEDVASPEGFARDPELVWKFYNERRR 70
SIR2_Af2 cd01413
SIR2_Af2: Archaeal and prokaryotic group which includes Archaeoglobus fulgidus Sir2-Af2, ...
 58-123 8.27e-20

SIR2_Af2: Archaeal and prokaryotic group which includes Archaeoglobus fulgidus Sir2-Af2, Sulfolobus solfataricus ssSir2, and several bacterial homologs; and are members of the SIR2 family of proteins, silent information regulator 2 (Sir2) enzymes which catalyze NAD+-dependent protein/histone deacetylation. Sir2 proteins have been shown to regulate gene silencing, DNA repair, metabolic enzymes, and life span. The Sir2 homolog from the archaea Sulfolobus solftaricus deacetylates the non-specific DNA protein Alba to mediate transcription repression.


Pssm-ID: 238704  Cd Length: 222  Bit Score: 80.88  E-value: 8.27e-20
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 149043899  58 LQECKKIIVLTGAGVSVSCGIPDFRSRDGIYARlavdfpdlPDPQAMFDIEYFRKDPRPFFKFAKK 123
Cdd:cd01413    1 LTKSRKTVVLTGAGISTESGIPDFRSPDGLWKK--------YDPEEVASIDYFYRNPEEFWRFYKE 58
SIR2 cd00296
SIR2 superfamily of proteins includes silent information regulator 2 (Sir2) enzymes which ...
 62-124 6.84e-19

SIR2 superfamily of proteins includes silent information regulator 2 (Sir2) enzymes which catalyze NAD+-dependent protein/histone deacetylation, where the acetyl group from the lysine epsilon-amino group is transferred to the ADP-ribose moiety of NAD+, producing nicotinamide and the novel metabolite O-acetyl-ADP-ribose. Sir2 proteins, also known as sirtuins, are found in all eukaryotes and many archaea and prokaryotes and have been shown to regulate gene silencing, DNA repair, metabolic enzymes, and life span. The most-studied function, gene silencing, involves the inactivation of chromosome domains containing key regulatory genes by packaging them into a specialized chromatin structure that is inaccessible to DNA-binding proteins. The oligomerization state of Sir2 appears to be organism-dependent, sometimes occurring as a monomer and sometimes as a multimer. Also included in this superfamily is a group of uncharacterized Sir2-like proteins which lack certain key catalytic residues and conserved zinc binding cysteines.


Pssm-ID: 238184 [Multi-domain]  Cd Length: 222  Bit Score: 78.54  E-value: 6.84e-19
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 149043899  62 KKIIVLTGAGVSVSCGIPDFRSRD-GIYARLAVDFPdlpdpqaMFDIEYFRKDPRPFFKFAKKK 124
Cdd:cd00296    1 KRVVVFTGAGISTESGIPDFRGLGtGLWTRLDPEEL-------AFSPEAFRRDPELFWLFYKER 57
PRK14138 PRK14138
NAD-dependent deacetylase; Provisional
 51-126 3.30e-15

NAD-dependent deacetylase; Provisional


Pssm-ID: 172627 [Multi-domain]  Cd Length: 244  Bit Score: 69.08  E-value: 3.30e-15
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 149043899  51 IEDAVKLLQECKKIIVLTGAGVSVSCGIPDFRSRDGIYARLavdfpdlpdPQAMFDIEYFRKDPRPFFKFAKKKQH 126
Cdd:PRK14138   1 MKEFLELLNESRLTVTLTGAGISTPSGIPDFRGPQGIYKKY---------PQNVFDIDFFYSHPEEFYRFAKEGIF 67
SIR2H cd01411
SIR2H: Uncharacterized prokaryotic Sir2 homologs from several gram positive bacterial species ...
 54-124 2.48e-14

SIR2H: Uncharacterized prokaryotic Sir2 homologs from several gram positive bacterial species and Fusobacteria; and are members of the SIR2 family of proteins, silent information regulator 2 (Sir2) enzymes which catalyze NAD+-dependent protein/histone deacetylation. Sir2 proteins have been shown to regulate gene silencing, DNA repair, metabolic enzymes, and life span.


Pssm-ID: 238702  Cd Length: 225  Bit Score: 66.24  E-value: 2.48e-14
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 149043899  54 AVKLLQECKKIIVLTGAGVSVSCGIPDFRSRDGIYARLAVDfpdlpDPQAMFDIEYFRKDPRPFFKFAKKK 124
Cdd:cd01411    1 LQHILKNAKRIVFFTGAGVSTASGIPDYRSKNGLYNEIYKY-----SPEYLLSHDFLEREPEKFYQFVKEN 66
PTZ00410 PTZ00410
NAD-dependent SIR2; Provisional
 62-122 1.65e-10

NAD-dependent SIR2; Provisional


Pssm-ID: 185600  Cd Length: 349  Bit Score: 56.80  E-value: 1.65e-10
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 149043899  62 KKIIVLTGAGVSVSCGIPDFRSRD-GIYARLAVdfPDLPDPQAMFDIEYFRKDPRPFFKFAK 122
Cdd:PTZ00410  30 TKILVMVGAGISVAAGIPDFRSPHtGIYAKLGK--YNLNSPTDAFSLTLLREKPEVFYSIAR 89
SIRT7 cd01410
SIRT7: Eukaryotic and prokaryotic group (class4) which includes human sirtuin SIRT6, SIRT7, ...
 62-94 3.75e-09

SIRT7: Eukaryotic and prokaryotic group (class4) which includes human sirtuin SIRT6, SIRT7, and several bacterial homologs; and are members of the SIR2 family of proteins, silent information regulator 2 (Sir2) enzymes which catalyze NAD+-dependent protein/histone deacetylation. Sir2 proteins have been shown to regulate gene silencing, DNA repair, metabolic enzymes, and life span.


Pssm-ID: 238701  Cd Length: 206  Bit Score: 52.30  E-value: 3.75e-09
                         10        20        30
                 ....*....|....*....|....*....|...
gi 149043899  62 KKIIVLTGAGVSVSCGIPDFRSRDGIYARLAVD 94
Cdd:cd01410    1 KHLVVFTGAGISTSAGIPDFRGPNGVWTLLPED 33
SIRT5_Af1_CobB cd01412
SIRT5_Af1_CobB: Eukaryotic, archaeal and prokaryotic group (class3) which includes human ...
 62-120 3.76e-09

SIRT5_Af1_CobB: Eukaryotic, archaeal and prokaryotic group (class3) which includes human sirtuin SIRT5, Archaeoglobus fulgidus Sir2-Af1, and E. coli CobB; and are members of the SIR2 family of proteins, silent information regulator 2 (Sir2) enzymes which catalyze NAD+-dependent protein/histone deacetylation. Sir2 proteins have been shown to regulate gene silencing, DNA repair, metabolic enzymes, and life span. CobB is a bacterial sirtuin that deacetylates acetyl-CoA synthetase at an active site lysine to stimulate its enzymatic activity.


Pssm-ID: 238703  Cd Length: 224  Bit Score: 52.21  E-value: 3.76e-09
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*....
gi 149043899  62 KKIIVLTGAGVSVSCGIPDFRSRDGIYARlaVDFPDLPDPQAmfdieyFRKDPRPFFKF 120
Cdd:cd01412    1 RRVVVLTGAGISAESGIPTFRDADGLWAR--FDPEELATPEA------FARDPELVWEF 51
SIRT4 cd01409
SIRT4: Eukaryotic and prokaryotic group (class2) which includes human sirtuin SIRT4 and ...
 55-90 5.24e-09

SIRT4: Eukaryotic and prokaryotic group (class2) which includes human sirtuin SIRT4 and several bacterial homologs; and are members of the SIR2 family of proteins, silent information regulator 2 (Sir2) enzymes which catalyze NAD+-dependent protein/histone deacetylation. Sir2 proteins have been shown to regulate gene silencing, DNA repair, metabolic enzymes, and life span.


Pssm-ID: 238700  Cd Length: 260  Bit Score: 52.30  E-value: 5.24e-09
                         10        20        30
                 ....*....|....*....|....*....|....*.
gi 149043899  55 VKLLQECKKIIVLTGAGVSVSCGIPDFRSRDGIYAR 90
Cdd:cd01409    2 QDFVARSRRLLVLTGAGISTESGIPDYRSEGGLYSR 37
PTZ00409 PTZ00409
Sir2 (Silent Information Regulator) protein; Provisional
 41-114 4.74e-07

Sir2 (Silent Information Regulator) protein; Provisional


Pssm-ID: 173599 [Multi-domain]  Cd Length: 271  Bit Score: 46.84  E-value: 4.74e-07
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 149043899  41 KRKKRKDInTIEDAVKLLQECKKIIVLTGAGVSVSCGIPDFR-SRDGIYARLavdfpdlpDPQAMFDIEYFRKDP 114
Cdd:PTZ00409   9 QKKVTKSI-TLEDLADMIRKCKYVVALTGSGTSAESNIPSFRgPSSSIWSKY--------DPKIYGTIWGFWKYP 74
PRK05333 PRK05333
NAD-dependent protein deacetylase;
63-90 6.18e-05

NAD-dependent protein deacetylase;


Pssm-ID: 235415  Cd Length: 285  Bit Score: 40.82  E-value: 6.18e-05
                         10        20
                 ....*....|....*....|....*...
gi 149043899  63 KIIVLTGAGVSVSCGIPDFRSRDGIYAR 90
Cdd:PRK05333  21 RLFVLTGAGISTDSGIPDYRDRNGQWKR 48
PTZ00408 PTZ00408
NAD-dependent deacetylase; Provisional
 61-100 6.61e-05

NAD-dependent deacetylase; Provisional


Pssm-ID: 240405  Cd Length: 242  Bit Score: 40.58  E-value: 6.61e-05
                         10        20        30        40
                 ....*....|....*....|....*....|....*....|
gi 149043899  61 CKKIIVLTGAGVSVSCGIPDFRSRDGIYARLAVDFPDLPD 100
Cdd:PTZ00408   4 CRCITILTGAGISAESGISTFRDGNGLWENHRVEDVATPD 43
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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