2-oxoacid:acceptor oxidoreductase, delta subunit, pyruvate/2-ketoisovalerate family; A number of anaerobic and microaerophilic species lack pyruvate dehydrogenase and have instead a four subunit, oxygen-sensitive pyruvate oxidoreductase, with either ferredoxins or flavodoxins used as the acceptor. Several related four-subunit enzymes may exist in the same species. This model describes a subfamily of delta subunits, representing mostly pyruvate, 2-ketoisovalerate, and 2-oxoglutarate specific enzymes. The delta subunit is the smallest and resembles ferredoxins.

                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 316893115   25 AGGTSKQFHTGEWRTRTPILDQDLCRQCMVCSMVCPDSSIPLKDGERVEFDYDHCKGCGICAKACPFDAITM 96
Cdd:TIGR02179   3 EPGSSVANKTGDWRVFKPVVDKEKCIKCKNCWLYCPEGAIQEDEGGFVGIDYDYCKGCGICANVCPVKAIEM 74

pyruvate synthase subunit PorD;

                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 316893115  27 GTSKQFHTGEWRTRTPILDQDLCRQCMVCSMVCPDSSIPLKDGERVEFDYDHCKGCGICAKACPFDAITM 96
Cdd:NF040684  12 GSTRKNKTGSWRTFRPILDKEKCIDCDNCYIFCPEGAIIQEENGMFEIDYDYCKGCGICANECPVKAIEM 81

EFR1 family ferrodoxin; Members of the family have a C-terminal ferrodoxin domain, with eight conserved Cys residues in two CxxCxxCxxxCP motifs, each of which binds a 4Fe-4S cluster. The N-terminal region resembles flavodoxin domains, with some members of the family recognized by Pfam models PF12724 (Flavodoxin_5) or PF00258 (Flavodoxin_1).

                         10        20
                 ....*....|....*....|....
gi 316893115  77 DHCKGCGICAKACPFDAITMKEGR 100
Cdd:NF038196 185 DKCIGCGICAKVCPVNNIEMEDGK 208

formate dehydrogenase FDH3 subunit beta; Members of this family are the beta subunit of the FDH3 type of formate dehydrogenase as found in Methylorubrum (Methylobacterium) extorquens.

                         10        20        30        40
                 ....*....|....*....|....*....|....*....|....*..
gi 316893115  52 CMVCS-----MVCPDSSIPLKDGERVEFDYDHCKGCGICAKACPFDA 93
Cdd:NF038355  54 CMHCTdapcaAVCPVDCFYIRADGIVLHDKDKCIGCGYCLYACPFGA 100

2-oxoacid:acceptor oxidoreductase, delta subunit, pyruvate/2-ketoisovalerate family; A number of anaerobic and microaerophilic species lack pyruvate dehydrogenase and have instead a four subunit, oxygen-sensitive pyruvate oxidoreductase, with either ferredoxins or flavodoxins used as the acceptor. Several related four-subunit enzymes may exist in the same species. This model describes a subfamily of delta subunits, representing mostly pyruvate, 2-ketoisovalerate, and 2-oxoglutarate specific enzymes. The delta subunit is the smallest and resembles ferredoxins.

                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 316893115   25 AGGTSKQFHTGEWRTRTPILDQDLCRQCMVCSMVCPDSSIPLKDGERVEFDYDHCKGCGICAKACPFDAITM 96
Cdd:TIGR02179   3 EPGSSVANKTGDWRVFKPVVDKEKCIKCKNCWLYCPEGAIQEDEGGFVGIDYDYCKGCGICANVCPVKAIEM 74

pyruvate synthase subunit PorD;

                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 316893115  27 GTSKQFHTGEWRTRTPILDQDLCRQCMVCSMVCPDSSIPLKDGERVEFDYDHCKGCGICAKACPFDAITM 96
Cdd:NF040684  12 GSTRKNKTGSWRTFRPILDKEKCIDCDNCYIFCPEGAIIQEENGMFEIDYDYCKGCGICANECPVKAIEM 81

pyruvate flavodoxin oxidoreductase subunit delta; Reviewed

                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 316893115  24 FAGGTSKQFHTGEWRTRTPILDQDLCRQCMVCSMVCPDSSIPLKDGERVEFDYDHCKGCGICAKACP 90
Cdd:PRK09625  36 YTEQSSFTTSVAHWRVEKPVHNNEICINCFNCWVYCPDAAILSRDKKLKGVDYSHCKGCGVCVEVCP 102

Heterodisulfide reductase, subunit A (polyferredoxin) [Energy production and conversion];

                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*..
gi 316893115  41 TPILDQDLCRQCMVCSMVCPDSSIPLKDGERVEFDYDHCKGCGICAKACPFDAITMK 97
Cdd:COG1148  490 VAEVDPEKCTGCGRCVEVCPYGAISIDEKGVAEVNPALCKGCGTCAAACPSGAISLK 546

archaeoflavoprotein, MJ0208 family; This model describes one of two paralogous families of archaealflavoprotein. The other, described by TIGR02699 and typified by the partially characterized AF1518 of Archaeoglobus fulgidus, is a homodimeric FMN-containing flavoprotein that accepts electrons from ferredoxin and can transfer them to various oxidoreductases. The function of this protein family is unknown. [Unknown function, General]

                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 316893115   35 GEWRTRTP-ILDQDLCRQCMVCSMVCPDSSIPLKDGeRVEFDYDHCKGCGICAKACPFDAI 94
Cdd:TIGR02700 135 GIIRTITPyMIDRKRCKGCGICVDACPRSAIDMVDG-KAFIRLLKCVGCGKCKEACPYNAI 194

Uncharacterized Fe-S cluster protein [Function unknown];

                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*...
gi 316893115  42 PILDQDLCRQCMVCSMVCPDSSIPLKDGeRVEFDYDHCKGCGICAKACPFDAITMKEG 99
Cdd:COG2768    6 PYVDEEKCIGCGACVKVCPVGAISIEDG-KAVIDPEKCIGCGACIEVCPVGAIKIEWE 62

Ferredoxin [Energy production and conversion];

                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*
gi 316893115  45 DQDLCRQCMVCSMVCPDSSIPLKDGER-VEFDYDHCKGCGICAKACPFDAITMKE 98
Cdd:COG1145  180 DAEKCIGCGLCVKVCPTGAIRLKDGKPqIVVDPDKCIGCGACVKVCPVGAISLEP 234

Uncharacterized polyferredoxin-like protein; This family contains uncharacterized polyferredoxin protein similar to Methanobacterium thermoautotrophicum MvhB. The mvhB is a gene of the methylviologen-reducing hydrogenase operon. It is predicted to contain 12 [4Fe-4S] clusters, and was therefore suggested to be a polyferredoxin. As a subfamily of the beta subunit of the DMSO Reductase (DMSOR) family, it is predicted to function as electron carrier in the reducing reaction.

                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 316893115  42 PILDQDLCRQCMVCSMVCPDSSIPLKDGE----RVEFDYDHCKGCGICAKACPFDAITMKE 98
Cdd:cd10549    1 LKYDPEKCIGCGICVKACPTDAIELGPNGaiarGPEIDEDKCVFCGACVEVCPTGAIELTP 61

TPP-dependent indolepyruvate ferredoxin oxidoreductase, alpha subunit [Energy production and conversion];

                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*
gi 316893115  45 DQDLCRQCMVCSMVCPDSSIPLKDGeRVEFDYDHCKGCGICAKACPFDAITMKEG 99
Cdd:COG4231   20 DEDKCTGCGACVKVCPADAIEEGDG-KAVIDPDLCIGCGSCVQVCPVDAIKLEKR 73

NAD-dependent dihydropyrimidine dehydrogenase, PreA subunit [Nucleotide transport and metabolism];

                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*....
gi 316893115  42 PILDQDLCRQCMVCSMVCPDSSI-PLKDGER-VEFDYDHCKGCGICAKACPFDAITMKE 98
Cdd:COG1146    3 PVIDTDKCIGCGACVEVCPVDVLeLDEEGKKaLVINPEECIGCGACELVCPVGAITVED 61

Iron only hydrogenase large subunit, C-terminal domain [Energy production and conversion];

                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|...
gi 316893115  45 DQDLCRQCMVCSMVCPDSSIPLKDGeRVEFDYDHCKGCGICAKACPFDAITMK 97
Cdd:COG4624   89 DKEKCKNCYPCVRACPVKAIKVDDG-KAEIDEEKCISCGQCVAVCPFGAITEK 140

HycB, HydN and similar proteins; This family includes HycB, the FeS subunit of a membrane-associated formate hydrogenlyase system (FHL-1) in Escherichia coli that breaks down formate, produced during anaerobic fermentation, to H2 and CO2. FHL-1 consists of formate dehydrogenase H (FDH-H) and the hydrogenase 3 complex (Hyd-3). HycB is thought to code for the [4Fe-4S] ferredoxin subunit of hydrogenase 3, which functions as an intermediate electron carrier protein between hydrogenase 3 and formate dehydrogenase. HydN codes for the [4Fe-4S] ferredoxin subunit of FDH-H; a hydN in-frame deletion mutation causes only weak reduction in hydrogenase activity, but loss of more than 60% of FDH-H activity. This pathway is only active at low pH and high formate concentrations, and is thought to provide a detoxification/de-acidification system countering the buildup of formate during fermentation.

                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|
gi 316893115  49 CRQC--MVCSMVCPDSSIPLKDGeRVEFDYDHCKGCGICAKACPFDAITM 96
Cdd:cd10554   56 CRQCedAPCANVCPVGAISQEDG-VVQVDEERCIGCKLCVLACPFGAIEM 104

Polyferredoxin NapH [Energy production and conversion];

                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|...
gi 316893115  45 DQDLCRQCMVCSMVCPdSSIPLKDGErveFDYDHCKGCGICAKACPFDAITMK 97
Cdd:COG0348  208 DRGDCIDCGLCVKVCP-MGIDIRKGE---INQSECINCGRCIDACPKDAIRFS 256

Beta subunit of the DMSO Reductase (DMSOR) family; This family consists of the small beta iron-sulfur (FeS) subunit of the DMSO Reductase (DMSOR) family. Members of this family also contain a large, periplasmic molybdenum-containing alpha subunit and may have a small gamma subunit as well. Examples of heterodimeric members with alpha and beta subunits include arsenite oxidase, and tungsten-containing formate dehydrogenase (FDH-T) while heterotrimeric members containing alpha, beta, and gamma subunits include formate dehydrogenase-N (FDH-N), and nitrate reductase (NarGHI). The beta subunit contains four Fe4/S4 and/or Fe3/S4 clusters which transfer the electrons from the alpha subunit to a hydrophobic integral membrane protein, presumably a cytochrome containing two b-type heme groups. The reducing equivalents are then transferred to menaquinone, which finally reduces the electron-accepting enzyme system.

                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|
gi 316893115  49 CRQCM--VCSMVCPDSSIPLKDGERVEFDYDHCKGCGICAKACPFDAITM 96
Cdd:cd04410   50 CMHCEdpPCVKACPTGAIYKDEDGIVLIDEDKCIGCGSCVEACPYGAIVF 99

uncharacterized subfamily of DMSO Reductase beta subunit family; This family consists of the small beta iron-sulfur (FeS) subunit of the DMSO Reductase (DMSOR) family. Members of this family also contain a large, periplasmic molybdenum-containing alpha subunit and may have a small gamma subunit as well. Examples of heterodimeric members with alpha and beta subunits include arsenite oxidase, and tungsten-containing formate dehydrogenase (FDH-T) while heterotrimeric members containing alpha, beta, and gamma subunits include formate dehydrogenase-N (FDH-N), and nitrate reductase (NarGHI). The beta subunit contains four Fe4/S4 and/or Fe3/S4 clusters which transfer the electrons from the alpha subunit to a hydrophobic integral membrane protein, presumably a cytochrome containing two b-type heme groups. The reducing equivalents are then transferred to menaquinone, which finally reduces the electron-accepting enzyme system.

                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....
gi 316893115  49 CRQC--MVCSMVCPDSSIPLKDGERVEFDYDHCKGCGICAKACPFDAITMKEGR 100
Cdd:cd16374   43 CRHCedAPCMEVCPTGAIYRDEDGAVLVDPDKCIGCGMCAMACPFGVPRFDPSL 96

NAD-dependent dihydropyrimidine dehydrogenase subunit PreA;

                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 316893115  42 PILDQDLCRQCMVCSMVCPDSS---IPL-KDGERV-EFDYDHCKGCGICAKACPFD-AITMKE 98
Cdd:PRK08318 337 ARIDQDKCIGCGRCYIACEDTShqaIEWdEDGTRTpEVIEEECVGCNLCAHVCPVEgCITMGE 399

uncharacterized subfamily of beta subunit of formate dehydrogenase; This subfamily includes the beta-subunit of formate dehydrogenases that are as yet uncharacterized. Members of the DMSO reductase family include formate dehydrogenase N and O (FDH-N, FDH-O) and tungsten-containing formate dehydrogenase (W-FDH) and other similar proteins. FDH-N, a major component of nitrate respiration of Escherichia coli, is involved in the major anaerobic respiratory pathway in the presence of nitrate, catalyzing the oxidation of formate to carbon dioxide at the expense of nitrate reduction to nitrite. It forms a heterotrimer; the alpha-subunit (FDH-G) is the catalytic site of formate oxidation and membrane-associated, incorporating a selenocysteine (SeCys) residue and a [4Fe/4S] cluster in addition to two bis-MGD cofactors, the beta subunit (FDH-H) contains four [4Fe/4S] clusters which transfer the electrons from the alpha subunit to the gamma-subunit (FDH-I), a hydrophobic integral membrane protein, presumably a cytochrome containing two b-type heme groups. W-FDH contains a tungsten instead of molybdenum at the catalytic center. This enzyme seems to be exclusively found in organisms such as hyperthermophilic archaea that live in extreme environments. It is a heterodimer of a large and a small subunit; the large subunit harbors the W site and one [4Fe-4S] center and the small subunit, containing three [4Fe-4S] clusters, functions to transfer electrons.

                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|
gi 316893115  50 RQCM-----VCSMVCPDSSIPLKDGERVEFDYDHCKGCGICAKACPFDAI 94
Cdd:cd10562   68 RQCMhctdaACVKVCPTGALYKTENGAVVVDEDKCIGCGYCVAACPFDVP 117

uncharacterized subfamily of DMSO Reductase beta subunit family; This family consists of the small beta iron-sulfur (FeS) subunit of the DMSO Reductase (DMSOR) family. Members of this family also contain a large, periplasmic molybdenum-containing alpha subunit and may have a small gamma subunit as well. Examples of heterodimeric members with alpha and beta subunits include arsenite oxidase, and tungsten-containing formate dehydrogenase (FDH-T) while heterotrimeric members containing alpha, beta, and gamma subunits include formate dehydrogenase-N (FDH-N), and nitrate reductase (NarGHI). The beta subunit contains four Fe4/S4 and/or Fe3/S4 clusters which transfer the electrons from the alpha subunit to a hydrophobic integral membrane protein, presumably a cytochrome containing two b-type heme groups. The reducing equivalents are then transferred to menaquinone, which finally reduces the electron-accepting enzyme system.

                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 316893115  40 RTPILDQDL--CRQCM-VCSMVCPDSSI-PLKDGER------VEFDYDHC------KGCGICAKACPFDAITMKE 98
Cdd:cd16373   44 RTPYLDPREgpCDLCCdACVEVCPTGALrPLDLEEQkvkmgvAVIDKDRClawqggTDCGVCVEACPTEAIAIVL 118

EFR1 family ferrodoxin; Members of the family have a C-terminal ferrodoxin domain, with eight conserved Cys residues in two CxxCxxCxxxCP motifs, each of which binds a 4Fe-4S cluster. The N-terminal region resembles flavodoxin domains, with some members of the family recognized by Pfam models PF12724 (Flavodoxin_5) or PF00258 (Flavodoxin_1).

                         10        20
                 ....*....|....*....|....
gi 316893115  77 DHCKGCGICAKACPFDAITMKEGR 100
Cdd:NF038196 185 DKCIGCGICAKVCPVNNIEMEDGK 208

Fe-S cluster domain-containing protein;

                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 316893115  42 PILDQDLCRQCMVCSMVCPDSSIPL-KDGERVefdYDHCK-----------------GCGICAKACPFDAITMKEGR 100
Cdd:PRK07118 163 PVVDEDKCTGCGACVKACPRNVIELiPKSARV---FVACNskdkgkavkkvcevgciGCGKCVKACPAGAITMENNL 236

indolepyruvate ferredoxin oxidoreductase, alpha subunit; Indolepyruvate ferredoxin oxidoreductase (IOR) is an alpha 2/beta 2 tetramer related to ketoacid oxidoreductases for pyruvate (1.2.7.1, POR), 2-ketoglutarate (1.2.7.3, KOR), and 2-oxoisovalerate (1.2.7.7, VOR). These multi-subunit enzymes typically are found in anaerobes and are inactiviated by oxygen. IOR in Pyrococcus acts in fermentation of all three aromatic amino acids, following removal of the amino group by transamination. In Methanococcus maripaludis, by contrast, IOR acts in the opposite direction, in pathways of amino acid biosynthesis from phenylacetate, indoleacetate, and p-hydroxyphenylacetate. In M. maripaludis and many other species, iorA and iorB are found next to an apparent phenylacetate-CoA ligase.

                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....
gi 316893115   43 ILDQDLCRQCMVC--SMVCPdsSIPLKDGErVEFDyDHCKGCGICAKACPFDAI 94
Cdd:TIGR03336 546 KVDQDKCIGCKKCikELGCP--AIEPEDKE-AVID-PLCTGCGVCAQICPFDAI 595

4Fe-4S dicluster domain; Superfamily includes proteins containing domains which bind to iron-sulfur clusters. Members include bacterial ferredoxins, various dehydrogenases, and various reductases. Structure of the domain is an alpha-antiparallel beta sandwich. Domain contains two 4Fe4S clusters.

                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*.
gi 316893115   45 DQDLCRQCMVCSMVCPDSSIP--LKDGERV-EFDYDHCKGCGICAKACP-FDAITM 96
Cdd:pfam14697   4 DEDTCIGCGKCYIACPDTSHQaiVGDGKRHhTVIEDECTGCNLCVSVCPvDDCITM 59

hypothetical protein; Provisional

                         10        20        30        40
                 ....*....|....*....|....*....|....*....|....
gi 316893115  48 LCRQCMVCSMVCPDSSIPLKDG-ERVEFDYDHCKGCGICAKACP 90
Cdd:PRK13795 582 ECVGCGVCVGACPTGAIRIEEGkRKISVDEEKCIHCGKCTEVCP 625

Fe-S cluster domain-containing protein;

                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|
gi 316893115  49 CRQCMVCSMVCPDSSIPLKDGeRVEFDYDHCKGCGICAKACPFDAITMKE 98
Cdd:PRK07118 215 CIGCGKCVKACPAGAITMENN-LAVIDQEKCTSCGKCVEKCPTKAIRILN 263

Ferredoxin [Energy production and conversion];

                         10        20        30
                 ....*....|....*....|....*....|....*..
gi 316893115  64 IPLKDGERVEFDYDHCKGCGICAKACPFDAITMKEGR 100
Cdd:COG1145  169 KIAIKKAKAVIDAEKCIGCGLCVKVCPTGAIRLKDGK 205

NapF, iron-sulfur subunit of periplasmic nitrate reductase; This family contains NapF protein, the iron-sulfur subunit of periplasmic nitrate reductase. The periplasmic nitrate reductase NapABC of Escherichia coli likely functions during anaerobic growth in low-nitrate environments; napF operon expression is activated by cyclic AMP receptor protein (Crp). NapF is a subfamily of the beta subunit of DMSO reductase (DMSOR) family. DMSOR family members have a large, periplasmic molybdenum-containing alpha subunit as well as a small beta FeS subunit, and may also have a small gamma subunit. The beta subunit contains four Fe4/S4 and/or Fe3/S4 clusters which transfer the electrons from the alpha subunit to a hydrophobic integral membrane protein, presumably a cytochrome containing two b-type heme groups. The reducing equivalents are then transferred to menaquinone, which finally reduces the electron-accepting enzyme system.

                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|.
gi 316893115  52 CMVCSMVCPDSSI---PLKDG-ERVEFDYDHCKGCGICAKACPFDAITMKE 98
Cdd:cd10564   88 CRSCQDACPTQAIrfrPRLGGiALPELDADACTGCGACVSVCPVGAITLTP 138

putative oxidoreductase; Provisional

                         10        20
                 ....*....|....*....|....
gi 316893115  75 DYDHCKGCGICAKACPFDAITMKE 98
Cdd:PRK13984  43 DWEKCIGCGTCSKICPTDAITMVE 66

Iron only hydrogenase large subunit, C-terminal domain [Energy production and conversion];

                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|..
gi 316893115  49 CRQCMVCSMVCPDSSIPLKDGERVEFDYDHCKGCGICAKACPFDAITMKEGR 100
Cdd:COG4624   63 RCCVAISCIQVRGIIIIDKRGPSIIRDKEKCKNCYPCVRACPVKAIKVDDGK 114

uncharacterized subfamily of DMSO Reductase beta subunit family; This family consists of the small beta iron-sulfur (FeS) subunit of the DMSO Reductase (DMSOR) family. Members of this family also contain a large, periplasmic molybdenum-containing alpha subunit and may have a small gamma subunit as well. Examples of heterodimeric members with alpha and beta subunits include arsenite oxidase, and tungsten-containing formate dehydrogenase (FDH-T) while heterotrimeric members containing alpha, beta, and gamma subunits include formate dehydrogenase-N (FDH-N), and nitrate reductase (NarGHI). The beta subunit contains four Fe4/S4 and/or Fe3/S4 clusters which transfer the electrons from the alpha subunit to a hydrophobic integral membrane protein, presumably a cytochrome containing two b-type heme groups. The reducing equivalents are then transferred to menaquinone, which finally reduces the electron-accepting enzyme system.

                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|..
gi 316893115  49 CRQCMVCSMVCPDSSIPLKDGERVEFDYDHCKGCGICAKACPFDAITMKEGR 100
Cdd:cd16372   49 CNQCGECIDVCPTGAITRDANGVVMINKKLCVGCLMCVGFCPEGAMFKHEDY 100

beta FeS subunits of formate dehydrogenase N (FDH-N) and similar proteins; This family contains beta FeS subunits of several dehydrogenases in the DMSO reductase superfamily, including formate dehydrogenase N (FDH-N), tungsten-containing formate dehydrogenase (W-FDH) and other similar proteins. FDH-N is a major component of nitrate respiration of Escherichia coli; it catalyzes the oxidation of formate to carbon dioxide, donating the electrons to a second substrate to a cytochrome. W-FDH contains a tungsten instead of molybdenum at the catalytic center and seems to be exclusively found in organisms such as hyperthermophilic archaea that live in extreme environments. It catalyzes the oxidation of formate to carbon dioxide, donating the electrons to a second substrate.

                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|
gi 316893115  50 RQCMVCS-----MVCPDSSIPLKDGERVEFDYDHCKGCGICAKACPFDAI 94
Cdd:cd16366   68 DQCMHCTdagclAACPTGAIIRTETGTVVVDPETCIGCGYCVNACPFDIP 117

hypothetical protein; Provisional

                         10        20
                 ....*....|....*....|..
gi 316893115  79 CKGCGICAKACPFDAITMKEGR 100
Cdd:PRK13795 583 CVGCGVCVGACPTGAIRIEEGK 604

uncharacterized subfamily of DMSO Reductase beta subunit family; This family consists of the small beta iron-sulfur (FeS) subunit of the DMSO Reductase (DMSOR) family. Members of this family also contain a large, periplasmic molybdenum-containing alpha subunit and may have a small gamma subunit as well. Examples of heterodimeric members with alpha and beta subunits include arsenite oxidase, and tungsten-containing formate dehydrogenase (FDH-T) while heterotrimeric members containing alpha, beta, and gamma subunits include formate dehydrogenase-N (FDH-N), and nitrate reductase (NarGHI). The beta subunit contains four Fe4/S4 and/or Fe3/S4 clusters which transfer the electrons from the alpha subunit to a hydrophobic integral membrane protein, presumably a cytochrome containing two b-type heme groups. The reducing equivalents are then transferred to menaquinone, which finally reduces the electron-accepting enzyme system.

                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*.
gi 316893115  43 ILDQDLCRQCMVCSMVCPDSSIPLKDGERVEFDydhCKGCGICAKACPFDAITMKE 98
Cdd:cd16372   73 MINKKLCVGCLMCVGFCPEGAMFKHEDYPEPFK---CIACGICVKACPTGALELVE 125

formate hydrogenlyase complex iron-sulfur subunit; Provisional

                         10        20
                 ....*....|....*....|....*
gi 316893115  73 EFDYDHCKGCGICAKACPFDAITMK 97
Cdd:PRK12387  34 EYNPQQCIGCAACVNACPSNALTVE 58

Na+-translocating ferredoxin:NAD+ oxidoreductase RNF, RnfB subunit [Energy production and conversion]; Na+-translocating ferredoxin:NAD+ oxidoreductase RNF, RnfB subunit is part of the Pathway/BioSystem: Na+-translocating Fd:NADH oxidoreductase

                         10        20
                 ....*....|....*....|...
gi 316893115  75 DYDHCKGCGICAKACPFDAITMK 97
Cdd:COG2878  165 DEDKCTGCGLCVEACPVDCIEMV 187

Acetyl-CoA synthase (ACS), also known as acetyl-CoA decarbonylase, is found in acetogenic and methanogenic organisms and is responsible for the synthesis and breakdown of acetyl-CoA. ACS forms a heterotetramer with carbon monoxide dehydrogenase (CODH) consisting of two ACS and two CODH subunits. CODH reduces carbon dioxide to carbon monoxide and ACS then synthesizes acetyl-CoA from carbon monoxide, CoA, and a methyl group donated by another protein (CoFeSP). ACS has three structural domains, an N-terminal rossman fold domain with a helical region at its N-terminus which interacts with CODH, and two alpha + beta fold domains. A Ni-Fe-S center referred to as the A-cluster is located in the C-terminal domain. A large cavity exists between the three domains which may bind CoA.

                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|.
gi 316893115  49 CRQCMVCSMVCPDSsIPLKDG---------ERVEFDYDHCKGCGICAKACP 90
Cdd:cd01916  367 CTDCGWCTRACPNS-LRIKEAmeaakegdfSGLADLFDQCVGCGRCEQECP 416

4Fe-4S dicluster domain;

                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....*....
gi 316893115   49 CRQCMVCSMVCPDSSIPLKDGE---RVEFDYDHCKGCGICAKACPFDAI 94
Cdd:pfam13187   2 CTGCGACVAACPAGAIVPDLVGqtiRGDIAGLACIGCGACVDACPRGAI 50

Fe-S cluster domain-containing protein;

                         10        20
                 ....*....|....*....|.
gi 316893115  79 CKGCGICAKACPFDAITMKEG 99
Cdd:PRK07118 141 CLGLGSCVAACPFDAIHIENG 161

Formate hydrogenlyase subunit 6/NADH:ubiquinone oxidoreductase 23 kD subunit (chain I) [Energy production and conversion]; Formate hydrogenlyase subunit 6/NADH:ubiquinone oxidoreductase 23 kD subunit (chain I) is part of the Pathway/BioSystem: NADH dehydrogenase

                         10        20
                 ....*....|....*....|....
gi 316893115  77 DHCKGCGICAKACPFDAITMKEGR 100
Cdd:COG1143    2 DKCIGCGLCVRVCPVDAITIEDGE 25

formate dehydrogenase FDH3 subunit beta; Members of this family are the beta subunit of the FDH3 type of formate dehydrogenase as found in Methylorubrum (Methylobacterium) extorquens.

                         10        20        30        40
                 ....*....|....*....|....*....|....*....|....*..
gi 316893115  52 CMVCS-----MVCPDSSIPLKDGERVEFDYDHCKGCGICAKACPFDA 93
Cdd:NF038355  54 CMHCTdapcaAVCPVDCFYIRADGIVLHDKDKCIGCGYCLYACPFGA 100

ferredoxin-like protein;

                         10        20        30        40
                 ....*....|....*....|....*....|....*....|....*...
gi 316893115  47 DLCRQCMV--CSMVCPDSSIPLKDGER-VEFDYDHCKGCGICAKACPF 91
Cdd:PRK09898 121 DTCRQCKEpqCMNVCPIGAITWQQKEGcITVDHKRCIGCSACTTACPW 168