|
Name |
Accession |
Description |
Interval |
E-value |
| PRK07059 |
PRK07059 |
Long-chain-fatty-acid--CoA ligase; Validated |
28-581 |
0e+00 |
|
Long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 235923 [Multi-domain] Cd Length: 557 Bit Score: 1147.84 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 28 VEKIWLKSYPAGVPHDINLSAATSISDMISASCRQFANSPAFTCMGKDISYKELDDHSRALAAWLQSRGLVKGDRVAIMM 107
Cdd:PRK07059 1 MEKIWLKSYPPGVPAEIDASQYPSLADLLEESFRQYADRPAFICMGKAITYGELDELSRALAAWLQSRGLAKGARVAIMM 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 108 PNILQYPITFTAVLRAGFVVVNVNPLYTPRELEHQLNDAGAKALVVLENFATTVEKALPSINVPNILVASMGDMLGFKGH 187
Cdd:PRK07059 81 PNVLQYPVAIAAVLRAGYVVVNVNPLYTPRELEHQLKDSGAEAIVVLENFATTVQQVLAKTAVKHVVVASMGDLLGFKGH 160
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 188 IVNLVVRRVKKMVPAWNIPGHVRFKDALAQGRAKSFNPVPVQGCDIAFLQYTGGTTGISKGAMLTHSNILANVEQMNLWM 267
Cdd:PRK07059 161 IVNFVVRRVKKMVPAWSLPGHVRFNDALAEGARQTFKPVKLGPDDVAFLQYTGGTTGVSKGATLLHRNIVANVLQMEAWL 240
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 268 DVAFRNKGKPKALNFVCALPLYHIFALTVNAMIGMKLGARNILIPNPRDIPSFVKELKKYPVHIFPGLNTLFNGLMNNPD 347
Cdd:PRK07059 241 QPAFEKKPRPDQLNFVCALPLYHIFALTVCGLLGMRTGGRNILIPNPRDIPGFIKELKKYQVHIFPAVNTLYNALLNNPD 320
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 348 FQTLDFKPLILTLGGGMAVQRPVAERWQHMTGCHITEGYGLSETSPVACANALDATEFTGTIGLPMPSTDVVIRDDDGSD 427
Cdd:PRK07059 321 FDKLDFSKLIVANGGGMAVQRPVAERWLEMTGCPITEGYGLSETSPVATCNPVDATEFSGTIGLPLPSTEVSIRDDDGND 400
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 428 LLLGKVGEICVRGPQVMKGYWNRPEETARAIMADGFFRTGDMGFMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVAAEH 507
Cdd:PRK07059 401 LPLGEPGEICIRGPQVMAGYWNRPDETAKVMTADGFFRTGDVGVMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVVASH 480
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 479816261 508 PGVLESAAVGIPNEHSGEVVKLYVVRRDPELTEDEVKAFCAERLTNYKRPREVEFRESLPKSNVGKILRRELRD 581
Cdd:PRK07059 481 PGVLEVAAVGVPDEHSGEAVKLFVVKKDPALTEEDVKAFCKERLTNYKRPKFVEFRTELPKTNVGKILRRELRD 554
|
|
| PRK08974 |
PRK08974 |
long-chain-fatty-acid--CoA ligase FadD; |
29-581 |
0e+00 |
|
long-chain-fatty-acid--CoA ligase FadD;
Pssm-ID: 236359 [Multi-domain] Cd Length: 560 Bit Score: 833.93 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 29 EKIWLKSYPAGVPHDINLSAATSISDMISASCRQFANSPAFTCMGKDISYKELDDHSRALAAWLQSR-GLVKGDRVAIMM 107
Cdd:PRK08974 2 EKVWLNRYPADVPAEINPDRYQSLVDMFEQAVARYADQPAFINMGEVMTFRKLEERSRAFAAYLQNGlGLKKGDRVALMM 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 108 PNILQYPITFTAVLRAGFVVVNVNPLYTPRELEHQLNDAGAKALVVLENFATTVEKALPSINVPNILVASMGDMLGF-KG 186
Cdd:PRK08974 82 PNLLQYPIALFGILRAGMIVVNVNPLYTPRELEHQLNDSGAKAIVIVSNFAHTLEKVVFKTPVKHVILTRMGDQLSTaKG 161
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 187 HIVNLVVRRVKKMVPAWNIPGHVRFKDALAQGRAKSFNPVPVQGCDIAFLQYTGGTTGISKGAMLTHSNILANVEQMNlW 266
Cdd:PRK08974 162 TLVNFVVKYIKRLVPKYHLPDAISFRSALHKGRRMQYVKPELVPEDLAFLQYTGGTTGVAKGAMLTHRNMLANLEQAK-A 240
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 267 MDVAFRNKGKPKAlnfVCALPLYHIFALTVNAMIGMKLGARNILIPNPRDIPSFVKELKKYPVHIFPGLNTLFNGLMNNP 346
Cdd:PRK08974 241 AYGPLLHPGKELV---VTALPLYHIFALTVNCLLFIELGGQNLLITNPRDIPGFVKELKKYPFTAITGVNTLFNALLNNE 317
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 347 DFQTLDFKPLILTLGGGMAVQRPVAERWQHMTGCHITEGYGLSETSPVACANALDATEFTGTIGLPMPSTDVVIRDDDGS 426
Cdd:PRK08974 318 EFQELDFSSLKLSVGGGMAVQQAVAERWVKLTGQYLLEGYGLTECSPLVSVNPYDLDYYSGSIGLPVPSTEIKLVDDDGN 397
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 427 DLLLGKVGEICVRGPQVMKGYWNRPEETARaIMADGFFRTGDMGFMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVAAE 506
Cdd:PRK08974 398 EVPPGEPGELWVKGPQVMLGYWQRPEATDE-VIKDGWLATGDIAVMDEEGFLRIVDRKKDMILVSGFNVYPNEIEDVVML 476
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 479816261 507 HPGVLESAAVGIPNEHSGEVVKLYVVRRDPELTEDEVKAFCAERLTNYKRPREVEFRESLPKSNVGKILRRELRD 581
Cdd:PRK08974 477 HPKVLEVAAVGVPSEVSGEAVKIFVVKKDPSLTEEELITHCRRHLTGYKVPKLVEFRDELPKSNVGKILRRELRD 551
|
|
| PRK05677 |
PRK05677 |
long-chain-fatty-acid--CoA ligase; Validated |
28-581 |
0e+00 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 168170 [Multi-domain] Cd Length: 562 Bit Score: 736.57 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 28 VEKIWLKSYPAGVPHDINLSAATSISDMISASCRQFANSPAFTCMGKDISYKELDDHSRALAAWLQSR-GLVKGDRVAIM 106
Cdd:PRK05677 2 IENFWKDKYPAGIAAEINPDEYPNIQAVLKQSCQRFADKPAFSNLGKTLTYGELYKLSGAFAAWLQQHtDLKPGDRIAVQ 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 107 MPNILQYPITFTAVLRAGFVVVNVNPLYTPRELEHQLNDAGAKALVVLENFATTVEKALPSINVPNILVASMGDMLG-FK 185
Cdd:PRK05677 82 LPNVLQYPVAVFGAMRAGLIVVNTNPLYTAREMEHQFNDSGAKALVCLANMAHLAEKVLPKTGVKHVIVTEVADMLPpLK 161
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 186 GHIVNLVVRRVKKMVPAWNIPGHVRFKDALAQGRAKSFNPVPVQGCDIAFLQYTGGTTGISKGAMLTHSNILANVEQMNL 265
Cdd:PRK05677 162 RLLINAVVKHVKKMVPAYHLPQAVKFNDALAKGAGQPVTEANPQADDVAVLQYTGGTTGVAKGAMLTHRNLVANMLQCRA 241
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 266 WMDVAFrNKGKPkalNFVCALPLYHIFALTVNAMIGMKLGARNILIPNPRDIPSFVKELKKYPVHIFPGLNTLFNGLMNN 345
Cdd:PRK05677 242 LMGSNL-NEGCE---ILIAPLPLYHIYAFTFHCMAMMLIGNHNILISNPRDLPAMVKELGKWKFSGFVGLNTLFVALCNN 317
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 346 PDFQTLDFKPLILTLGGGMAVQRPVAERWQHMTGCHITEGYGLSETSPVACANALDATEFtGTIGLPMPSTDVVIRDDDG 425
Cdd:PRK05677 318 EAFRKLDFSALKLTLSGGMALQLATAERWKEVTGCAICEGYGMTETSPVVSVNPSQAIQV-GTIGIPVPSTLCKVIDDDG 396
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 426 SDLLLGKVGEICVRGPQVMKGYWNRPEETARAIMADGFFRTGDMGFMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVAA 505
Cdd:PRK05677 397 NELPLGEVGELCVKGPQVMKGYWQRPEATDEILDSDGWLKTGDIALIQEDGYMRIVDRKKDMILVSGFNVYPNELEDVLA 476
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 479816261 506 EHPGVLESAAVGIPNEHSGEVVKLYVVRR-DPELTEDEVKAFCAERLTNYKRPREVEFRESLPKSNVGKILRRELRD 581
Cdd:PRK05677 477 ALPGVLQCAAIGVPDEKSGEAIKVFVVVKpGETLTKEQVMEHMRANLTGYKVPKAVEFRDELPTTNVGKILRRELRD 553
|
|
| PRK08751 |
PRK08751 |
long-chain fatty acid--CoA ligase; |
23-581 |
0e+00 |
|
long-chain fatty acid--CoA ligase;
Pssm-ID: 181546 [Multi-domain] Cd Length: 560 Bit Score: 733.99 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 23 EARPavekiWLKSYPAGVPHDINLSAATSISDMISASCRQFANSPAFTCMGKDISYKELDDHSRALAAWLQSR-GLVKGD 101
Cdd:PRK08751 3 QARP-----WLQSYPAGVAAEIDLEQFRTVAEVFATSVAKFADRPAYHSFGKTITYREADQLVEQFAAYLLGElQLKKGD 77
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 102 RVAIMMPNILQYPITFTAVLRAGFVVVNVNPLYTPRELEHQLNDAGAKALVVLENFATTVEKALPSINVPNILVASMGDM 181
Cdd:PRK08751 78 RVALMMPNCLQYPIATFGVLRAGLTVVNVNPLYTPRELKHQLIDSGASVLVVIDNFGTTVQQVIADTPVKQVITTGLGDM 157
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 182 LGF-KGHIVNLVVRRVKKMVPAWNIPGHVRFKDALAQGRAKSFNPVPVQGCDIAFLQYTGGTTGISKGAMLTHSNILANV 260
Cdd:PRK08751 158 LGFpKAALVNFVVKYVKKLVPEYRINGAIRFREALALGRKHSMPTLQIEPDDIAFLQYTGGTTGVAKGAMLTHRNLVANM 237
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 261 EQMNLWMDVAfrNKGKPKALNFVCALPLYHIFALTVNAMIGMKLGARNILIPNPRDIPSFVKELKKYPVHIFPGLNTLFN 340
Cdd:PRK08751 238 QQAHQWLAGT--GKLEEGCEVVITALPLYHIFALTANGLVFMKIGGCNHLISNPRDMPGFVKELKKTRFTAFTGVNTLFN 315
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 341 GLMNNPDFQTLDFKPLILTLGGGMAVQRPVAERWQHMTGCHITEGYGLSETSPVACANALDATEFTGTIGLPMPSTDVVI 420
Cdd:PRK08751 316 GLLNTPGFDQIDFSSLKMTLGGGMAVQRSVAERWKQVTGLTLVEAYGLTETSPAACINPLTLKEYNGSIGLPIPSTDACI 395
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 421 RDDDGSDLLLGKVGEICVRGPQVMKGYWNRPEETARAIMADGFFRTGDMGFMDERGYTKIVDRKKDMILVSGFNVYPNEI 500
Cdd:PRK08751 396 KDDAGTVLAIGEIGELCIKGPQVMKGYWKRPEETAKVMDADGWLHTGDIARMDEQGFVYIVDRKKDMILVSGFNVYPNEI 475
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 501 EEVAAEHPGVLESAAVGIPNEHSGEVVKLYVVRRDPELTEDEVKAFCAERLTNYKRPREVEFRESLPKSNVGKILRRELR 580
Cdd:PRK08751 476 EDVIAMMPGVLEVAAVGVPDEKSGEIVKVVIVKKDPALTAEDVKAHARANLTGYKQPRIIEFRKELPKTNVGKILRRELR 555
|
.
gi 479816261 581 D 581
Cdd:PRK08751 556 D 556
|
|
| PRK12492 |
PRK12492 |
long-chain-fatty-acid--CoA ligase; Provisional |
32-581 |
0e+00 |
|
long-chain-fatty-acid--CoA ligase; Provisional
Pssm-ID: 171539 [Multi-domain] Cd Length: 562 Bit Score: 685.01 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 32 WLKSYPAGVPHDINLSAATSISDMISASCRQFANSPAFTCMGKDISYKELDDHSRALAAWLQSR-GLVKGDRVAIMMPNI 110
Cdd:PRK12492 6 WNDKRPAGVPSTIDLAAYKSVVEVFERSCKKFADRPAFSNLGVTLSYAELERHSAAFAAYLQQHtDLVPGDRIAVQMPNV 85
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 111 LQYPITFTAVLRAGFVVVNVNPLYTPRELEHQLNDAGAKALVVLENFATTVEKALPSINVPNILVASMGDML-GFKGHIV 189
Cdd:PRK12492 86 LQYPIAVFGALRAGLIVVNTNPLYTAREMRHQFKDSGARALVYLNMFGKLVQEVLPDTGIEYLIEAKMGDLLpAAKGWLV 165
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 190 NLVVRRVKKMVPAWNIPGHVRFKDALAQGRAKSFNPVPVQGCDIAFLQYTGGTTGISKGAMLTHSNILANVEQMNLWMDv 269
Cdd:PRK12492 166 NTVVDKVKKMVPAYHLPQAVPFKQALRQGRGLSLKPVPVGLDDIAVLQYTGGTTGLAKGAMLTHGNLVANMLQVRACLS- 244
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 270 AFRNKGKPKALN----FVCALPLYHIFALTVNAMIGMKLGARNILIPNPRDIPSFVKELKKYPVHIFPGLNTLFNGLMNN 345
Cdd:PRK12492 245 QLGPDGQPLMKEgqevMIAPLPLYHIYAFTANCMCMMVSGNHNVLITNPRDIPGFIKELGKWRFSALLGLNTLFVALMDH 324
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 346 PDFQTLDFKPLILTLGGGMAVQRPVAERWQHMTGCHITEGYGLSETSPVACANALDATEFTGTIGLPMPSTDVVIRDDDG 425
Cdd:PRK12492 325 PGFKDLDFSALKLTNSGGTALVKATAERWEQLTGCTIVEGYGLTETSPVASTNPYGELARLGTVGIPVPGTALKVIDDDG 404
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 426 SDLLLGKVGEICVRGPQVMKGYWNRPEETARAIMADGFFRTGDMGFMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVAA 505
Cdd:PRK12492 405 NELPLGERGELCIKGPQVMKGYWQQPEATAEALDAEGWFKTGDIAVIDPDGFVRIVDRKKDLIIVSGFNVYPNEIEDVVM 484
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 479816261 506 EHPGVLESAAVGIPNEHSGEVVKLYVVRRDPELTEDEVKAFCAERLTNYKRPREVEFRESLPKSNVGKILRRELRD 581
Cdd:PRK12492 485 AHPKVANCAAIGVPDERSGEAVKLFVVARDPGLSVEELKAYCKENFTGYKVPKHIVLRDSLPMTPVGKILRRELRD 560
|
|
| FC-FACS_FadD_like |
cd05936 |
Prokaryotic long-chain fatty acid CoA synthetases similar to Escherichia coli FadD; This ... |
52-580 |
0e+00 |
|
Prokaryotic long-chain fatty acid CoA synthetases similar to Escherichia coli FadD; This subfamily of the AMP-forming adenylation family contains Escherichia coli FadD and similar prokaryotic fatty acid CoA synthetases. FadD was characterized as a long-chain fatty acid CoA synthetase. The gene fadD is regulated by the fatty acid regulatory protein FadR. Fatty acid CoA synthetase catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, followed by the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341259 [Multi-domain] Cd Length: 468 Bit Score: 671.97 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 52 ISDMISASCRQFANSPAFTCMGKDISYKELDDHSRALAAWLQSRGLVKGDRVAIMMPNILQYPITFTAVLRAGFVVVNVN 131
Cdd:cd05936 1 LADLLEEAARRFPDKTALIFMGRKLTYRELDALAEAFAAGLQNLGVQPGDRVALMLPNCPQFPIAYFGALKAGAVVVPLN 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 132 PLYTPRELEHQLNDAGAKALVVlenfattvekalpsinvpnilvasmgdmlgfkghivnlvvrrvkkmvpawnipgHVRF 211
Cdd:cd05936 81 PLYTPRELEHILNDSGAKALIV------------------------------------------------------AVSF 106
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 212 KDALAQGRAKSFNPVPVQGcDIAFLQYTGGTTGISKGAMLTHSNILANVEQMNLWMdvafrNKGKPKALNFVCALPLYHI 291
Cdd:cd05936 107 TDLLAAGAPLGERVALTPE-DVAVLQYTSGTTGVPKGAMLTHRNLVANALQIKAWL-----EDLLEGDDVVLAALPLFHV 180
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 292 FALTVNAMIGMKLGARNILIPNPRDIpSFVKELKKYPVHIFPGLNTLFNGLMNNPDFQTLDFKPLILTLGGGMAVQRPVA 371
Cdd:cd05936 181 FGLTVALLLPLALGATIVLIPRFRPI-GVLKEIRKHRVTIFPGVPTMYIALLNAPEFKKRDFSSLRLCISGGAPLPVEVA 259
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 372 ERWQHMTGCHITEGYGLSETSPVACANALDATEFTGTIGLPMPSTDVVIRDDDGSDLLLGKVGEICVRGPQVMKGYWNRP 451
Cdd:cd05936 260 ERFEELTGVPIVEGYGLTETSPVVAVNPLDGPRKPGSIGIPLPGTEVKIVDDDGEELPPGEVGELWVRGPQVMKGYWNRP 339
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 452 EETARAIMaDGFFRTGDMGFMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVAAEHPGVLESAAVGIPNEHSGEVVKLYV 531
Cdd:cd05936 340 EETAEAFV-DGWLRTGDIGYMDEDGYFFIVDRKKDMIIVGGFNVYPREVEEVLYEHPAVAEAAVVGVPDPYSGEAVKAFV 418
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|
gi 479816261 532 VRRDPE-LTEDEVKAFCAERLTNYKRPREVEFRESLPKSNVGKILRRELR 580
Cdd:cd05936 419 VLKEGAsLTEEEIIAFCREQLAGYKVPRQVEFRDELPKSAVGKILRRELR 468
|
|
| MenE/FadK |
COG0318 |
O-succinylbenzoic acid-CoA ligase MenE or related acyl-CoA synthetase (AMP-forming) [Lipid ... |
52-581 |
0e+00 |
|
O-succinylbenzoic acid-CoA ligase MenE or related acyl-CoA synthetase (AMP-forming) [Lipid transport and metabolism]; O-succinylbenzoic acid-CoA ligase MenE or related acyl-CoA synthetase (AMP-forming) is part of the Pathway/BioSystem: Menaquinone biosynthesis
Pssm-ID: 440087 [Multi-domain] Cd Length: 452 Bit Score: 548.26 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 52 ISDMISASCRQFANSPAFTCMGKDISYKELDDHSRALAAWLQSRGLVKGDRVAIMMPNILQYPITFTAVLRAGFVVVNVN 131
Cdd:COG0318 1 LADLLRRAAARHPDRPALVFGGRRLTYAELDARARRLAAALRALGVGPGDRVALLLPNSPEFVVAFLAALRAGAVVVPLN 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 132 PLYTPRELEHQLNDAGAKALVVlenfattvekalpsinvpnilvasmgdmlgfkghivnlvvrrvkkmvpawnipghvrf 211
Cdd:COG0318 81 PRLTAEELAYILEDSGARALVT---------------------------------------------------------- 102
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 212 kdalaqgraksfnpvpvqgcdiAFLQYTGGTTGISKGAMLTHSNILANVEQMNLWMDVafrnkgkPKALNFVCALPLYHI 291
Cdd:COG0318 103 ----------------------ALILYTSGTTGRPKGVMLTHRNLLANAAAIAAALGL-------TPGDVVLVALPLFHV 153
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 292 FALTVNAMIGMKLGARNILIPNpRDIPSFVKELKKYPVHIFPGLNTLFNGLMNNPDFQTLDFKPLILTLGGGMAVQRPVA 371
Cdd:COG0318 154 FGLTVGLLAPLLAGATLVLLPR-FDPERVLELIERERVTVLFGVPTMLARLLRHPEFARYDLSSLRLVVSGGAPLPPELL 232
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 372 ERWQHMTGCHITEGYGLSETSPVACANALDATE-FTGTIGLPMPSTDVVIRDDDGSDLLLGKVGEICVRGPQVMKGYWNR 450
Cdd:COG0318 233 ERFEERFGVRIVEGYGLTETSPVVTVNPEDPGErRPGSVGRPLPGVEVRIVDEDGRELPPGEVGEIVVRGPNVMKGYWND 312
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 451 PEETARAiMADGFFRTGDMGFMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVAAEHPGVLESAAVGIPNEHSGEVVKLY 530
Cdd:COG0318 313 PEATAEA-FRDGWLRTGDLGRLDEDGYLYIVGRKKDMIISGGENVYPAEVEEVLAAHPGVAEAAVVGVPDEKWGERVVAF 391
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|..
gi 479816261 531 VVRRD-PELTEDEVKAFCAERLTNYKRPREVEFRESLPKSNVGKILRRELRD 581
Cdd:COG0318 392 VVLRPgAELDAEELRAFLRERLARYKVPRRVEFVDELPRTASGKIDRRALRE 443
|
|
| PRK05605 |
PRK05605 |
long-chain-fatty-acid--CoA ligase; Validated |
19-581 |
5.29e-164 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 235531 [Multi-domain] Cd Length: 573 Bit Score: 479.50 E-value: 5.29e-164
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 19 APTGEARPAVEKIWLKSYPAGVPHDINLSAaTSISDMISASCRQFANSPAFTCMGKDISYKELDDHSRALAAWLQSRGLV 98
Cdd:PRK05605 2 APSQEMSAFADKPWLQSYAPWTPHDLDYGD-TTLVDLYDNAVARFGDRPALDFFGATTTYAELGKQVRRAAAGLRALGVR 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 99 KGDRVAIMMPNILQYPITFTAVLRAGFVVVNVNPLYTPRELEHQLNDAGAKALVVLENFATTVEKALPSINVPNILVASM 178
Cdd:PRK05605 81 PGDRVAIVLPNCPQHIVAFYAVLRLGAVVVEHNPLYTAHELEHPFEDHGARVAIVWDKVAPTVERLRRTTPLETIVSVNM 160
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 179 GD-MLGFKGHIVNLVVRRVKKM-----VPAwniPGHVRFKDALAQGRAKSFNPVP---VQGCDIAFLQYTGGTTGISKGA 249
Cdd:PRK05605 161 IAaMPLLQRLALRLPIPALRKAraaltGPA---PGTVPWETLVDAAIGGDGSDVShprPTPDDVALILYTSGTTGKPKGA 237
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 250 MLTHSNILANVEQMNLWMdvafrNKGKPKALNFVCALPLYHIFALTVNAMIGMKLGARNILIPNPrDIPSFVKELKKYPV 329
Cdd:PRK05605 238 QLTHRNLFANAAQGKAWV-----PGLGDGPERVLAALPMFHAYGLTLCLTLAVSIGGELVLLPAP-DIDLILDAMKKHPP 311
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 330 HIFPGLNTLFNGLMNNPDFQTLDFKPLILTLGGGMAVQRPVAERWQHMTGCHITEGYGLSETSPVACANALDATEFTGTI 409
Cdd:PRK05605 312 TWLPGVPPLYEKIAEAAEERGVDLSGVRNAFSGAMALPVSTVELWEKLTGGLLVEGYGLTETSPIIVGNPMSDDRRPGYV 391
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 410 GLPMPSTDVVI--RDDDGSDLLLGKVGEICVRGPQVMKGYWNRPEETArAIMADGFFRTGDMGFMDERGYTKIVDRKKDM 487
Cdd:PRK05605 392 GVPFPDTEVRIvdPEDPDETMPDGEEGELLVRGPQVFKGYWNRPEETA-KSFLDGWFRTGDVVVMEEDGFIRIVDRIKEL 470
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 488 ILVSGFNVYPNEIEEVAAEHPGVLESAAVGIPNEHSGE-VVKLYVVRRDPELTEDEVKAFCAERLTNYKRPREVEFRESL 566
Cdd:PRK05605 471 IITGGFNVYPAEVEEVLREHPGVEDAAVVGLPREDGSEeVVAAVVLEPGAALDPEGLRAYCREHLTRYKVPRRFYHVDEL 550
|
570
....*....|....*
gi 479816261 567 PKSNVGKILRRELRD 581
Cdd:PRK05605 551 PRDQLGKVRRREVRE 565
|
|
| PRK07656 |
PRK07656 |
long-chain-fatty-acid--CoA ligase; Validated |
47-581 |
2.05e-151 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 236072 [Multi-domain] Cd Length: 513 Bit Score: 445.12 E-value: 2.05e-151
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 47 SAATSISDMISASCRQFANSPAFTCMGKDISYKELDDHSRALAAWLQSRGLVKGDRVAIMMPNILQYPITFTAVLRAGFV 126
Cdd:PRK07656 2 NEWMTLPELLARAARRFGDKEAYVFGDQRLTYAELNARVRRAAAALAALGIGKGDRVAIWAPNSPHWVIAALGALKAGAV 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 127 VVNVNPLYTPRELEHQLNDAGAKALVVLENFATTVEKALPSinVPNIlvasmgdmlgfkGHIVNLVVRRvkkmvPAWNIP 206
Cdd:PRK07656 82 VVPLNTRYTADEAAYILARGDAKALFVLGLFLGVDYSATTR--LPAL------------EHVVICETEE-----DDPHTE 142
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 207 GHVRFKDALAQGrAKSFNPVPVQGCDIAFLQYTGGTTGISKGAMLTHSNILANVEQmnlWMDVAFRNKGKpkalNFVCAL 286
Cdd:PRK07656 143 KMKTFTDFLAAG-DPAERAPEVDPDDVADILFTSGTTGRPKGAMLTHRQLLSNAAD---WAEYLGLTEGD----RYLAAN 214
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 287 PLYHIFALTVNAMIGMKLGArnILIPNPR-DIPSFVKELKKYPVHIFPGLNTLFNGLMNNPDFQTLDFKPLILTLGGGMA 365
Cdd:PRK07656 215 PFFHVFGYKAGVNAPLMRGA--TILPLPVfDPDEVFRLIETERITVLPGPPTMYNSLLQHPDRSAEDLSSLRLAVTGAAS 292
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 366 VqrPVA--ERWQHMTGC-HITEGYGLSETSPVACANALD--ATEFTGTIGLPMPSTDVVIRDDDGSDLLLGKVGEICVRG 440
Cdd:PRK07656 293 M--PVAllERFESELGVdIVLTGYGLSEASGVTTFNRLDddRKTVAGTIGTAIAGVENKIVNELGEEVPVGEVGELLVRG 370
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 441 PQVMKGYWNRPEETARAIMADGFFRTGDMGFMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVAAEHPGVLESAAVGIPN 520
Cdd:PRK07656 371 PNVMKGYYDDPEATAAAIDADGWLHTGDLGRLDEEGYLYIVDRKKDMFIVGGFNVYPAEVEEVLYEHPAVAEAAVIGVPD 450
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|..
gi 479816261 521 EHSGEVVKLYVVRRD-PELTEDEVKAFCAERLTNYKRPREVEFRESLPKSNVGKILRRELRD 581
Cdd:PRK07656 451 ERLGEVGKAYVVLKPgAELTEEELIAYCREHLAKYKVPRSIEFLDELPKNATGKVLKRALRE 512
|
|
| PRK06187 |
PRK06187 |
long-chain-fatty-acid--CoA ligase; Validated |
51-581 |
2.95e-137 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 235730 [Multi-domain] Cd Length: 521 Bit Score: 409.19 E-value: 2.95e-137
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 51 SISDMISASCRQFANSPAFTCMGKDISYKELDDHSRALAAWLQSRGLVKGDRVAIMMPNILQYPITFTAVLRAGFVVVNV 130
Cdd:PRK06187 7 TIGRILRHGARKHPDKEAVYFDGRRTTYAELDERVNRLANALRALGVKKGDRVAVFDWNSHEYLEAYFAVPKIGAVLHPI 86
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 131 NPLYTPRELEHQLNDAGAKALVVLENFATTVEKALPSI-NVPNILVASMGDMLGfkghivnlvvrrvkkmvpawNIPGHV 209
Cdd:PRK06187 87 NIRLKPEEIAYILNDAEDRVVLVDSEFVPLLAAILPQLpTVRTVIVEGDGPAAP--------------------LAPEVG 146
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 210 RFKDALAqGRAKSFNPVPVQGCDIAFLQYTGGTTGISKGAMLTHSNILANVEQMNLWMDVAFRNKGKPkalnfvcALPLY 289
Cdd:PRK06187 147 EYEELLA-AASDTFDFPDIDENDAAAMLYTSGTTGHPKGVVLSHRNLFLHSLAVCAWLKLSRDDVYLV-------IVPMF 218
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 290 HIFALTVnAMIGMKLGARNILIPnpRDIPSFVKEL-KKYPVHIFPGLNTLFNGLMNNPDFQTLDFKPLILTLGGGMAVQR 368
Cdd:PRK06187 219 HVHAWGL-PYLALMAGAKQVIPR--RFDPENLLDLiETERVTFFFAVPTIWQMLLKAPRAYFVDFSSLRLVIYGGAALPP 295
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 369 PVAERWQHMTGCHITEGYGLSETSPVACANALDA-----TEFTGTIGLPMPSTDVVIRDDDGSDL--LLGKVGEICVRGP 441
Cdd:PRK06187 296 ALLREFKEKFGIDLVQGYGMTETSPVVSVLPPEDqlpgqWTKRRSAGRPLPGVEARIVDDDGDELppDGGEVGEIIVRGP 375
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 442 QVMKGYWNRPEETARAImADGFFRTGDMGFMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVAAEHPGVLESAAVGIPNE 521
Cdd:PRK06187 376 WLMQGYWNRPEATAETI-DGGWLHTGDVGYIDEDGYLYITDRIKDVIISGGENIYPRELEDALYGHPAVAEVAVIGVPDE 454
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|.
gi 479816261 522 HSGEVVKLYVV-RRDPELTEDEVKAFCAERLTNYKRPREVEFRESLPKSNVGKILRRELRD 581
Cdd:PRK06187 455 KWGERPVAVVVlKPGATLDAKELRAFLRGRLAKFKLPKRIAFVDELPRTSVGKILKRVLRE 515
|
|
| FACL_FadD13-like |
cd17631 |
fatty acyl-CoA synthetase, including FadD13; This family contains fatty acyl-CoA synthetases, ... |
59-576 |
7.29e-136 |
|
fatty acyl-CoA synthetase, including FadD13; This family contains fatty acyl-CoA synthetases, including Mycobacterium tuberculosis acid-induced operon MymA encoding the fatty acyl-CoA synthetase FadD13 which is essential for virulence and intracellular growth of the pathogen. The fatty acyl-CoA synthetase activates lipids before entering into the metabolic pathways and is also involved in transmembrane lipid transport. However, unlike soluble fatty acyl-CoA synthetases, but like the mammalian integral-membrane very-long-chain acyl-CoA synthetases, FadD13 accepts lipid substrates up to the maximum length of C26, and this is facilitated by an extensive hydrophobic tunnel from the active site to a positively charged patch. Also included is feruloyl-CoA synthetase (Fcs) in Rhodococcus strains where it is involved in biotechnological vanillin production from eugenol and ferulic acid via a non-beta-oxidative pathway.
Pssm-ID: 341286 [Multi-domain] Cd Length: 435 Bit Score: 402.37 E-value: 7.29e-136
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 59 SCRQFANSPAFTCMGKDISYKELDDHSRALAAWLQSRGLVKGDRVAIMMPNILQYPITFTAVLRAGFVVVNVNPLYTPRE 138
Cdd:cd17631 4 RARRHPDRTALVFGGRSLTYAELDERVNRLAHALRALGVAKGDRVAVLSKNSPEFLELLFAAARLGAVFVPLNFRLTPPE 83
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 139 LEHQLNDAGAKalVVLEnfattvekalpsinvpnilvasmgdmlgfkghivnlvvrrvkkmvpawnipghvrfkdalaqg 218
Cdd:cd17631 84 VAYILADSGAK--VLFD--------------------------------------------------------------- 98
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 219 raksfnpvpvqgcDIAFLQYTGGTTGISKGAMLTHSNILANVeqMNLWMDVAFRNKGKpkalnFVCALPLYHIFALTVNA 298
Cdd:cd17631 99 -------------DLALLMYTSGTTGRPKGAMLTHRNLLWNA--VNALAALDLGPDDV-----LLVVAPLFHIGGLGVFT 158
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 299 MIGMKLGARNILIPNPrDIPSFVKELKKYPVHIFPGLNTLFNGLMNNPDFQTLDFKPLILTLGGGMAVQRPVAERWQhMT 378
Cdd:cd17631 159 LPTLLRGGTVVILRKF-DPETVLDLIERHRVTSFFLVPTMIQALLQHPRFATTDLSSLRAVIYGGAPMPERLLRALQ-AR 236
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 379 GCHITEGYGLSETSPVACAN-ALDATEFTGTIGLPMPSTDVVIRDDDGSDLLLGKVGEICVRGPQVMKGYWNRPEETARA 457
Cdd:cd17631 237 GVKFVQGYGMTETSPGVTFLsPEDHRRKLGSAGRPVFFVEVRIVDPDGREVPPGEVGEIVVRGPHVMAGYWNRPEATAAA 316
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 458 ImADGFFRTGDMGFMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVAAEHPGVLESAAVGIPNEHSGEVVKLYVVRRDP- 536
Cdd:cd17631 317 F-RDGWFHTGDLGRLDEDGYLYIVDRKKDMIISGGENVYPAEVEDVLYEHPAVAEVAVIGVPDEKWGEAVVAVVVPRPGa 395
|
490 500 510 520
....*....|....*....|....*....|....*....|
gi 479816261 537 ELTEDEVKAFCAERLTNYKRPREVEFRESLPKSNVGKILR 576
Cdd:cd17631 396 ELDEDELIAHCRERLARYKIPKSVEFVDALPRNATGKILK 435
|
|
| PRK06710 |
PRK06710 |
long-chain-fatty-acid--CoA ligase; Validated |
28-579 |
4.36e-135 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 180666 [Multi-domain] Cd Length: 563 Bit Score: 405.19 E-value: 4.36e-135
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 28 VEKIWLKSYPAGVPHDINLSAaTSISDMISASCRQFANSPAFTCMGKDISYKELDDHSRALAAWLQSRGLVKGDRVAIMM 107
Cdd:PRK06710 3 VEKPWLKSYPEEIPSTISYDI-QPLHKYVEQMASRYPEKKALHFLGKDITFSVFHDKVKRFANYLQKLGVEKGDRVAIML 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 108 PNILQYPITFTAVLRAGFVVVNVNPLYTPRELEHQLNDAGAKALVVLENFATTVEKALPSINVPNILVASMGDMLGFKGH 187
Cdd:PRK06710 82 PNCPQAVIGYYGTLLAGGIVVQTNPLYTERELEYQLHDSGAKVILCLDLVFPRVTNVQSATKIEHVIVTRIADFLPFPKN 161
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 188 IV---------NLVVR-RVKKMVPAWNipghvrfkdALAQGRAKSFNPVPVQGCDIAFLQYTGGTTGISKGAMLTHSNIL 257
Cdd:PRK06710 162 LLypfvqkkqsNLVVKvSESETIHLWN---------SVEKEVNTGVEVPCDPENDLALLQYTGGTTGFPKGVMLTHKNLV 232
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 258 ANVEQMNLWMDVAfrNKGKPKALNfvcALPLYHIFALTVNAMIGMKLGARNILIPNpRDIPSFVKELKKYPVHIFPGLNT 337
Cdd:PRK06710 233 SNTLMGVQWLYNC--KEGEEVVLG---VLPFFHVYGMTAVMNLSIMQGYKMVLIPK-FDMKMVFEAIKKHKVTLFPGAPT 306
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 338 LFNGLMNNPDFQTLDFKPLILTLGGGMAVQRPVAERWQHMTGCHITEGYGLSETSPVACANALDATEFTGTIGLPMPSTD 417
Cdd:PRK06710 307 IYIALLNSPLLKEYDISSIRACISGSAPLPVEVQEKFETVTGGKLVEGYGLTESSPVTHSNFLWEKRVPGSIGVPWPDTE 386
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 418 VVIRD-DDGSDLLLGKVGEICVRGPQVMKGYWNRPEETArAIMADGFFRTGDMGFMDERGYTKIVDRKKDMILVSGFNVY 496
Cdd:PRK06710 387 AMIMSlETGEALPPGEIGEIVVKGPQIMKGYWNKPEETA-AVLQDGWLHTGDVGYMDEDGFFYVKDRKKDMIVASGFNVY 465
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 497 PNEIEEVAAEHPGVLESAAVGIPNEHSGEVVKLYVV-RRDPELTEDEVKAFCAERLTNYKRPREVEFRESLPKSNVGKIL 575
Cdd:PRK06710 466 PREVEEVLYEHEKVQEVVTIGVPDPYRGETVKAFVVlKEGTECSEEELNQFARKYLAAYKVPKVYEFRDELPKTTVGKIL 545
|
....
gi 479816261 576 RREL 579
Cdd:PRK06710 546 RRVL 549
|
|
| Firefly_Luc_like |
cd05911 |
Firefly luciferase of light emitting insects and 4-Coumarate-CoA Ligase (4CL); This family ... |
73-575 |
7.82e-130 |
|
Firefly luciferase of light emitting insects and 4-Coumarate-CoA Ligase (4CL); This family contains insect firefly luciferases that share significant sequence similarity to plant 4-coumarate:coenzyme A ligases, despite their functional diversity. Luciferase catalyzes the production of light in the presence of MgATP, molecular oxygen, and luciferin. In the first step, luciferin is activated by acylation of its carboxylate group with ATP, resulting in an enzyme-bound luciferyl adenylate. In the second step, luciferyl adenylate reacts with molecular oxygen, producing an enzyme-bound excited state product (Luc=O*) and releasing AMP. This excited-state product then decays to the ground state (Luc=O), emitting a quantum of visible light.
Pssm-ID: 341237 [Multi-domain] Cd Length: 486 Bit Score: 388.88 E-value: 7.82e-130
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 73 GKDISYKELDDHSRALAAWLQSRGLVKGDRVAIMMPNILQYPITFTAVLRAGFVVVNVNPLYTPRELEHQLNDAGAKALV 152
Cdd:cd05911 8 GKELTYAQLRTLSRRLAAGLRKLGLKKGDVVGIISPNSTYYPPVFLGCLFAGGIFSAANPIYTADELAHQLKISKPKVIF 87
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 153 VLENFATTVEKALPS-INVPNILVasMGDMLGFKGHIVNLVVrrvkkmvPAWNIPGHVRFKDalaQGRAKSfnpvpvqgc 231
Cdd:cd05911 88 TDPDGLEKVKEAAKElGPKDKIIV--LDDKPDGVLSIEDLLS-------PTLGEEDEDLPPP---LKDGKD--------- 146
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 232 DIAFLQYTGGTTGISKGAMLTHSNILANVEQMNLWMDVAFRNKGKpkalnFVCALPLYHIFALTvNAMIGMKLGARNILI 311
Cdd:cd05911 147 DTAAILYSSGTTGLPKGVCLSHRNLIANLSQVQTFLYGNDGSNDV-----ILGFLPLYHIYGLF-TTLASLLNGATVIIM 220
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 312 PNPrDIPSFVKELKKYPV---HIFPglnTLFNGLMNNPDFQTLDFKPLILTLGGGMAVQRPVAERWQHM-TGCHITEGYG 387
Cdd:cd05911 221 PKF-DSELFLDLIEKYKItflYLVP---PIAAALAKSPLLDKYDLSSLRVILSGGAPLSKELQELLAKRfPNATIKQGYG 296
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 388 LSETSPVACANaLDATEFTGTIGLPMPSTDVVIRDDDGSDLL-LGKVGEICVRGPQVMKGYWNRPEETARAIMADGFFRT 466
Cdd:cd05911 297 MTETGGILTVN-PDGDDKPGSVGRLLPNVEAKIVDDDGKDSLgPNEPGEICVRGPQVMKGYYNNPEATKETFDEDGWLHT 375
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 467 GDMGFMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVAAEHPGVLESAAVGIPNEHSGEVVKLYVVRRD-PELTEDEVKA 545
Cdd:cd05911 376 GDIGYFDEDGYLYIVDRKKELIKYKGFQVAPAELEAVLLEHPGVADAAVIGIPDEVSGELPRAYVVRKPgEKLTEKEVKD 455
|
490 500 510
....*....|....*....|....*....|.
gi 479816261 546 FCAERLTNYKRPR-EVEFRESLPKSNVGKIL 575
Cdd:cd05911 456 YVAKKVASYKQLRgGVVFVDEIPKSASGKIL 486
|
|
| PRK08314 |
PRK08314 |
long-chain-fatty-acid--CoA ligase; Validated |
40-581 |
2.37e-127 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 236235 [Multi-domain] Cd Length: 546 Bit Score: 384.70 E-value: 2.37e-127
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 40 VPHDINLSAaTSISDMISASCRQFANSPAFTCMGKDISYKELDDHSRALAAWLQSR-GLVKGDRVAIMMPNILQYPITFT 118
Cdd:PRK08314 1 LPKSLTLPE-TSLFHNLEVSARRYPDKTAIVFYGRAISYRELLEEAERLAGYLQQEcGVRKGDRVLLYMQNSPQFVIAYY 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 119 AVLRAGFVVVNVNPLYTPRELEHQLNDAGAKALVVLENFATTVEKALPSINVPNILVASMGDMLGFKGHIVNLVVRRVKK 198
Cdd:PRK08314 80 AILRANAVVVPVNPMNREEELAHYVTDSGARVAIVGSELAPKVAPAVGNLRLRHVIVAQYSDYLPAEPEIAVPAWLRAEP 159
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 199 MVPAWNIPGHVRFKDALAQGRAksfnPVPVQGC--DIAFLQYTGGTTGISKGAMLTHSNILANVEQMNLWMDVAFRNkgk 276
Cdd:PRK08314 160 PLQALAPGGVVAWKEALAAGLA----PPPHTAGpdDLAVLPYTSGTTGVPKGCMHTHRTVMANAVGSVLWSNSTPES--- 232
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 277 pkalNFVCALPLYHI--FALTVNAMIGMklGARNILIPN-PRDIpsfVKEL-KKYPVHIFPGLNTLFNGLMNNPDFQTLD 352
Cdd:PRK08314 233 ----VVLAVLPLFHVtgMVHSMNAPIYA--GATVVLMPRwDREA---AARLiERYRVTHWTNIPTMVVDFLASPGLAERD 303
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 353 FKPLILTLGGGMAVQRPVAERWQHMTGCHITEGYGLSETSPVACANALDATEfTGTIGLPMPSTDVVIRD-DDGSDLLLG 431
Cdd:PRK08314 304 LSSLRYIGGGGAAMPEAVAERLKELTGLDYVEGYGLTETMAQTHSNPPDRPK-LQCLGIPTFGVDARVIDpETLEELPPG 382
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 432 KVGEICVRGPQVMKGYWNRPEETARAIMA-DG--FFRTGDMGFMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVAAEHP 508
Cdd:PRK08314 383 EVGEIVVHGPQVFKGYWNRPEATAEAFIEiDGkrFFRTGDLGRMDEEGYFFITDRLKRMINASGFKVWPAEVENLLYKHP 462
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 479816261 509 GVLESAAVGIPNEHSGEVVKLYVVRRDP---ELTEDEVKAFCAERLTNYKRPREVEFRESLPKSNVGKILRRELRD 581
Cdd:PRK08314 463 AIQEACVIATPDPRRGETVKAVVVLRPEargKTTEEEIIAWAREHMAAYKYPRIVEFVDSLPKSGSGKILWRQLQE 538
|
|
| AMP-binding |
pfam00501 |
AMP-binding enzyme; |
58-491 |
3.26e-125 |
|
AMP-binding enzyme;
Pssm-ID: 459834 [Multi-domain] Cd Length: 417 Bit Score: 374.34 E-value: 3.26e-125
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 58 ASCRQFANSPAFTCM-GKDISYKELDDHSRALAAWLQSRGLVKGDRVAIMMPNILQYPITFTAVLRAGFVVVNVNPLYTP 136
Cdd:pfam00501 3 RQAARTPDKTALEVGeGRRLTYRELDERANRLAAGLRALGVGKGDRVAILLPNSPEWVVAFLACLKAGAVYVPLNPRLPA 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 137 RELEHQLNDAGAKALVVLENF-ATTVEKALPSINVPNILVASMGDMLGFKGHIVNLVVRRVKKMVPawnipghvrfkdal 215
Cdd:pfam00501 83 EELAYILEDSGAKVLITDDALkLEELLEALGKLEVVKLVLVLDRDPVLKEEPLPEEAKPADVPPPP-------------- 148
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 216 aqgraksfnPVPVQGCDIAFLQYTGGTTGISKGAMLTHSNILANVEQMNLWMDVAFRNKGKPKalnFVCALPLYHIFALT 295
Cdd:pfam00501 149 ---------PPPPDPDDLAYIIYTSGTTGKPKGVMLTHRNLVANVLSIKRVRPRGFGLGPDDR---VLSTLPLFHDFGLS 216
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 296 VNAMIGMKLGARNILIP--NPRDIPSFVKELKKYPVHIFPGLNTLFNGLMNNPDFQTLDFKPLILTLGGGMAVQRPVAER 373
Cdd:pfam00501 217 LGLLGPLLAGATVVLPPgfPALDPAALLELIERYKVTVLYGVPTLLNMLLEAGAPKRALLSSLRLVLSGGAPLPPELARR 296
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 374 WQHMTGCHITEGYGLSETSPVACANALDATEFT--GTIGLPMPSTDVVIRDDDGSDLL-LGKVGEICVRGPQVMKGYWNR 450
Cdd:pfam00501 297 FRELFGGALVNGYGLTETTGVVTTPLPLDEDLRslGSVGRPLPGTEVKIVDDETGEPVpPGEPGELCVRGPGVMKGYLND 376
|
410 420 430 440
....*....|....*....|....*....|....*....|.
gi 479816261 451 PEETARAIMADGFFRTGDMGFMDERGYTKIVDRKKDMILVS 491
Cdd:pfam00501 377 PELTAEAFDEDGWYRTGDLGRRDEDGYLEIVGRKKDQIKLG 417
|
|
| 4CL |
cd05904 |
4-Coumarate-CoA Ligase (4CL); 4-Coumarate:coenzyme A ligase is a key enzyme in the ... |
73-579 |
1.36e-117 |
|
4-Coumarate-CoA Ligase (4CL); 4-Coumarate:coenzyme A ligase is a key enzyme in the phenylpropanoid metabolic pathway for monolignol and flavonoid biosynthesis. It catalyzes the synthesis of hydroxycinnamate-CoA thioesters in a two-step reaction, involving the formation of hydroxycinnamate-AMP anhydride and the nucleophilic substitution of AMP by CoA. The phenylpropanoid pathway is one of the most important secondary metabolism pathways in plants and hydroxycinnamate-CoA thioesters are the precursors of lignin and other important phenylpropanoids.
Pssm-ID: 341230 [Multi-domain] Cd Length: 505 Bit Score: 358.09 E-value: 1.36e-117
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 73 GKDISYKELDDHSRALAAWLQSRGLVKGDRVAIMMPNILQYPITFTAVLRAGFVVVNVNPLYTPRELEHQLNDAGAKALV 152
Cdd:cd05904 30 GRALTYAELERRVRRLAAGLAKRGGRKGDVVLLLSPNSIEFPVAFLAVLSLGAVVTTANPLSTPAEIAKQVKDSGAKLAF 109
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 153 VLenfATTVEKaLPSINVPNILVASMgdmlgfkgHIVNLVVRRVKKMVPAWNIPghvrfkdalaqgraksfnPVPVQGCD 232
Cdd:cd05904 110 TT---AELAEK-LASLALPVVLLDSA--------EFDSLSFSDLLFEADEAEPP------------------VVVIKQDD 159
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 233 IAFLQYTGGTTGISKGAMLTHSNILANVEQMnlwmdVAFRNKGKPKALNFVCALPLYHIFALTVNAMIGMKLGARNILIP 312
Cdd:cd05904 160 VAALLYSSGTTGRSKGVMLTHRNLIAMVAQF-----VAGEGSNSDSEDVFLCVLPMFHIYGLSSFALGLLRLGATVVVMP 234
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 313 NpRDIPSFVKELKKYPVHIFPGLNTLFNGLMNNPDFQTLDFKPLILTLGGGMAVQRPVAERW-QHMTGCHITEGYGLSET 391
Cdd:cd05904 235 R-FDLEELLAAIERYKVTHLPVVPPIVLALVKSPIVDKYDLSSLRQIMSGAAPLGKELIEAFrAKFPNVDLGQGYGMTES 313
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 392 SPVA--CANALDATEFTGTIGLPMPSTDVVIRD-DDGSDLLLGKVGEICVRGPQVMKGYWNRPEETARAIMADGFFRTGD 468
Cdd:cd05904 314 TGVVamCFAPEKDRAKYGSVGRLVPNVEAKIVDpETGESLPPNQTGELWIRGPSIMKGYLNNPEATAATIDKEGWLHTGD 393
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 469 MGFMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVAAEHPGVLESAAVGIPNEHSGEVVKLYVVRR-DPELTEDEVKAFC 547
Cdd:cd05904 394 LCYIDEDGYLFIVDRLKELIKYKGFQVAPAELEALLLSHPEILDAAVIPYPDEEAGEVPMAFVVRKpGSSLTEDEIMDFV 473
|
490 500 510
....*....|....*....|....*....|..
gi 479816261 548 AERLTNYKRPREVEFRESLPKSNVGKILRREL 579
Cdd:cd05904 474 AKQVAPYKKVRKVAFVDAIPKSPSGKILRKEL 505
|
|
| AFD_class_I |
cd04433 |
Adenylate forming domain, Class I, also known as the ANL superfamily; This family is known as ... |
232-575 |
1.18e-113 |
|
Adenylate forming domain, Class I, also known as the ANL superfamily; This family is known as the ANL (acyl-CoA synthetases, the NRPS adenylation domains, and the Luciferase enzymes) superfamily. It includes acyl- and aryl-CoA ligases, as well as the adenylation domain of nonribosomal peptide synthetases and firefly luciferases.The adenylate-forming enzymes catalyze an ATP-dependent two-step reaction to first activate a carboxylate substrate as an adenylate and then transfer the carboxylate to the pantetheine group of either coenzyme A or an acyl-carrier protein. The active site of the domain is located at the interface of a large N-terminal subdomain and a smaller C-terminal subdomain.
Pssm-ID: 341228 [Multi-domain] Cd Length: 336 Bit Score: 341.96 E-value: 1.18e-113
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 232 DIAFLQYTGGTTGISKGAMLTHSNILANVEQMNLWMDVAFRNKgkpkalnFVCALPLYHIFALTVnAMIGMKLGARNILI 311
Cdd:cd04433 1 DPALILYTSGTTGKPKGVVLSHRNLLAAAAALAASGGLTEGDV-------FLSTLPLFHIGGLFG-LLGALLAGGTVVLL 72
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 312 PnPRDIPSFVKELKKYPVHIFPGLNTLFNGLMNNPDFQTLDFKPLILTLGGGMAVQRPVAERWQHMTGCHITEGYGLSET 391
Cdd:cd04433 73 P-KFDPEAALELIEREKVTILLGVPTLLARLLKAPESAGYDLSSLRALVSGGAPLPPELLERFEEAPGIKLVNGYGLTET 151
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 392 S-PVACANALDATEFTGTIGLPMPSTDVVIRDDDGSDLLLGKVGEICVRGPQVMKGYWNRPEETArAIMADGFFRTGDMG 470
Cdd:cd04433 152 GgTVATGPPDDDARKPGSVGRPVPGVEVRIVDPDGGELPPGEIGELVVRGPSVMKGYWNNPEATA-AVDEDGWYRTGDLG 230
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 471 FMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVAAEHPGVLESAAVGIPNEHSGEVVKLYVVRRDPE-LTEDEVKAFCAE 549
Cdd:cd04433 231 RLDEDGYLYIVGRLKDMIKSGGENVYPAEVEAVLLGHPGVAEAAVVGVPDPEWGERVVAVVVLRPGAdLDAEELRAHVRE 310
|
330 340
....*....|....*....|....*.
gi 479816261 550 RLTNYKRPREVEFRESLPKSNVGKIL 575
Cdd:cd04433 311 RLAPYKVPRRVVFVDALPRTASGKID 336
|
|
| LC_FACS_like |
cd05935 |
Putative long-chain fatty acid CoA ligase; The members of this family are putative long-chain ... |
76-579 |
2.14e-108 |
|
Putative long-chain fatty acid CoA ligase; The members of this family are putative long-chain fatty acyl-CoA synthetases, which catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. Fatty acyl-CoA synthetases are responsible for fatty acid degradation as well as physiological regulation of cellular functions via the production of fatty acyl-CoA esters.
Pssm-ID: 341258 [Multi-domain] Cd Length: 430 Bit Score: 331.75 E-value: 2.14e-108
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 76 ISYKELDDHSRALAAWLQSRGLVKGDRVAIMMPNILQYPITFTAVLRAGFVVVNVNPLYTPRELEHQLNDAGAKALVVLE 155
Cdd:cd05935 2 LTYLELLEVVKKLASFLSNKGVRKGDRVGICLQNSPQYVIAYFAIWRANAVVVPINPMLKERELEYILNDSGAKVAVVGS 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 156 NFAttvekalpsinvpnilvasmgdmlgfkghivnlvvrrvkkmvpawnipghvrfkdalaqgraksfnpvpvqgcDIAF 235
Cdd:cd05935 82 ELD-------------------------------------------------------------------------DLAL 88
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 236 LQYTGGTTGISKGAMLTHSNILANVEQMNLWmdvafrNKGKPKALnFVCALPLYHIFALTVNAMIGMKLGARnILIPNPR 315
Cdd:cd05935 89 IPYTSGTTGLPKGCMHTHFSAAANALQSAVW------TGLTPSDV-ILACLPLFHVTGFVGSLNTAVYVGGT-YVLMARW 160
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 316 DIPSFVKELKKYPVHIFPGLNTLFNGLMNNPDFQTLDFKPLILTLGGGMAVQRPVAERWQHMTGCHITEGYGLSETSPVA 395
Cdd:cd05935 161 DRETALELIEKYKVTFWTNIPTMLVDLLATPEFKTRDLSSLKVLTGGGAPMPPAVAEKLLKLTGLRFVEGYGLTETMSQT 240
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 396 CANALDATEFTgTIGLPMPSTDVVIRD-DDGSDLLLGKVGEICVRGPQVMKGYWNRPEETARAIMADG---FFRTGDMGF 471
Cdd:cd05935 241 HTNPPLRPKLQ-CLGIP*FGVDARVIDiETGRELPPNEVGEIVVRGPQIFKGYWNRPEETEESFIEIKgrrFFRTGDLGY 319
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 472 MDERGYTKIVDRKKDMILVSGFNVYPNEIEEVAAEHPGVLESAAVGIPNEHSGEVVKLYVVRRdPEL----TEDEVKAFC 547
Cdd:cd05935 320 MDEEGYFFFVDRVKRMINVSGFKVWPAEVEAKLYKHPAI*EVCVISVPDERVGEEVKAFIVLR-PEYrgkvTEEDIIEWA 398
|
490 500 510
....*....|....*....|....*....|..
gi 479816261 548 AERLTNYKRPREVEFRESLPKSNVGKILRREL 579
Cdd:cd05935 399 REQMAAYKYPREVEFVDELPRSASGKILWRLL 430
|
|
| PRK08316 |
PRK08316 |
acyl-CoA synthetase; Validated |
41-581 |
8.03e-104 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 181381 [Multi-domain] Cd Length: 523 Bit Score: 323.04 E-value: 8.03e-104
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 41 PHDINLSAATSISDMISASCRQFANSPAFTCMGKDISYKELDDHSRALAAWLQSRGLVKGDRVAIMMPNILQYPITFTAV 120
Cdd:PRK08316 2 MERSTRARRQTIGDILRRSARRYPDKTALVFGDRSWTYAELDAAVNRVAAALLDLGLKKGDRVAALGHNSDAYALLWLAC 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 121 LRAGFVVVNVNPLYTPRELEHQLNDAGAKALVVLENFATTVEKALPSINVpnilvasmgdmlgfkghiVNLVVRRVKKMV 200
Cdd:PRK08316 82 ARAGAVHVPVNFMLTGEELAYILDHSGARAFLVDPALAPTAEAALALLPV------------------DTLILSLVLGGR 143
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 201 PAwniPGHVRFKDALAQGRAKSFNPVPVQGCDIAFLQYTGGTTGISKGAMLTHSNILAnvEQMNLWMDVAFRNKGKPkal 280
Cdd:PRK08316 144 EA---PGGWLDFADWAEAGSVAEPDVELADDDLAQILYTSGTESLPKGAMLTHRALIA--EYVSCIVAGDMSADDIP--- 215
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 281 nfVCALPLYHIFALTVNAMIGMKLGARNILIPNPrDIPSFVKELKKYPVHIFPGLNTLFNGLMNNPDFQTLDFKPLILTL 360
Cdd:PRK08316 216 --LHALPLYHCAQLDVFLGPYLYVGATNVILDAP-DPELILRTIEAERITSFFAPPTVWISLLRHPDFDTRDLSSLRKGY 292
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 361 GGGMAVQRPVAERWQH-MTGCHITEGYGLSETSPVACA-NALDATEFTGTIGLPMPSTDVVIRDDDGSDLLLGKVGEICV 438
Cdd:PRK08316 293 YGASIMPVEVLKELRErLPGLRFYNCYGQTEIAPLATVlGPEEHLRRPGSAGRPVLNVETRVVDDDGNDVAPGEVGEIVH 372
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 439 RGPQVMKGYWNRPEETARAiMADGFFRTGDMGFMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVAAEHPGVLESAAVGI 518
Cdd:PRK08316 373 RSPQLMLGYWDDPEKTAEA-FRGGWFHSGDLGVMDEEGYITVVDRKKDMIKTGGENVASREVEEALYTHPAVAEVAVIGL 451
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....
gi 479816261 519 PNEHSGEVVKLYVVRRD-PELTEDEVKAFCAERLTNYKRPREVEFRESLPKSNVGKILRRELRD 581
Cdd:PRK08316 452 PDPKWIEAVTAVVVPKAgATVTEDELIAHCRARLAGFKVPKRVIFVDELPRNPSGKILKRELRE 515
|
|
| Acs |
COG0365 |
Acyl-coenzyme A synthetase/AMP-(fatty) acid ligase [Lipid transport and metabolism]; |
74-581 |
2.32e-101 |
|
Acyl-coenzyme A synthetase/AMP-(fatty) acid ligase [Lipid transport and metabolism];
Pssm-ID: 440134 [Multi-domain] Cd Length: 565 Bit Score: 317.82 E-value: 2.32e-101
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 74 KDISYKELDDHSRALAAWLQSRGLVKGDRVAIMMPNILQYPITFTAVLRAGFVVVNVNPLYTPRELEHQLNDAGAKALVV 153
Cdd:COG0365 38 RTLTYAELRREVNRFANALRALGVKKGDRVAIYLPNIPEAVIAMLACARIGAVHSPVFPGFGAEALADRIEDAEAKVLIT 117
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 154 ----LENFATTVEKAlpsiNVPNILvasmgDMLGFKGHIVnlVVRRVKKMVPawnIPGHVRFKDALAQGrAKSFNPVPVQ 229
Cdd:COG0365 118 adggLRGGKVIDLKE----KVDEAL-----EELPSLEHVI--VVGRTGADVP---MEGDLDWDELLAAA-SAEFEPEPTD 182
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 230 GCDIAFLQYTGGTTGISKGAMLTHSNILANVE-QMNLWMDVafrnkgKPKALnFVCALPLYHIFALTvNAMIG-MKLGAR 307
Cdd:COG0365 183 ADDPLFILYTSGTTGKPKGVVHTHGGYLVHAAtTAKYVLDL------KPGDV-FWCTADIGWATGHS-YIVYGpLLNGAT 254
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 308 NIL---IPNPRDIPSFVKELKKYPVHIF---PglnTLFNGLMNNPDF--QTLDFKPLILTLGGGMAVQRPVAERWQHMTG 379
Cdd:COG0365 255 VVLyegRPDFPDPGRLWELIEKYGVTVFftaP---TAIRALMKAGDEplKKYDLSSLRLLGSAGEPLNPEVWEWWYEAVG 331
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 380 CHITEGYGLSET-SPVACANALDATEFtGTIGLPMPSTDVVIRDDDGSDLLLGKVGEICVRGPQ--VMKGYWNRPEETAR 456
Cdd:COG0365 332 VPIVDGWGQTETgGIFISNLPGLPVKP-GSMGKPVPGYDVAVVDEDGNPVPPGEEGELVIKGPWpgMFRGYWNDPERYRE 410
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 457 AI--MADGFFRTGDMGFMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVAAEHPGVLESAAVGIPNEHSGEVVKLYVVRR 534
Cdd:COG0365 411 TYfgRFPGWYRTGDGARRDEDGYFWILGRSDDVINVSGHRIGTAEIESALVSHPAVAEAAVVGVPDEIRGQVVKAFVVLK 490
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|..
gi 479816261 535 -----DPELtEDEVKAFCAERLTNYKRPREVEFRESLPKSNVGKILRRELRD 581
Cdd:COG0365 491 pgvepSDEL-AKELQAHVREELGPYAYPREIEFVDELPKTRSGKIMRRLLRK 541
|
|
| PRK06178 |
PRK06178 |
acyl-CoA synthetase; Validated |
28-579 |
2.45e-100 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 235724 [Multi-domain] Cd Length: 567 Bit Score: 315.44 E-value: 2.45e-100
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 28 VEKIWLKSYPAGVPHDIN-LSAATSISDMISASCRQFANSPAFTCMGKDISYKELDDHSRALAAWLQSRGLVKGDRVAIM 106
Cdd:PRK06178 10 LRALQQAAWPAGIPREPEyPHGERPLTEYLRAWARERPQRPAIIFYGHVITYAELDELSDRFAALLRQRGVGAGDRVAVF 89
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 107 MPNILQYPITFTAVLRAGFVVVNVNPLYTPRELEHQLNDAGAKALVVLENFATTVEKALPSINVPNILVASMGDMLGFKG 186
Cdd:PRK06178 90 LPNCPQFHIVFFGILKLGAVHVPVSPLFREHELSYELNDAGAEVLLALDQLAPVVEQVRAETSLRHVIVTSLADVLPAEP 169
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 187 HIVnlvvrrVKKMV--PAWNIPGHVRFKDALAQGRAKSFNPVPVQGcDIAFLQYTGGTTGISKGAMLTHSNIlanVEQMN 264
Cdd:PRK06178 170 TLP------LPDSLraPRLAAAGAIDLLPALRACTAPVPLPPPALD-ALAALNYTGGTTGMPKGCEHTQRDM---VYTAA 239
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 265 LWMDVAFRNKGKPKALNFvcaLPLYHIFALTVNAMIGMKLGARNILIpNPRDIPSFVKELKKYPVHIFPGLNTLFNGLMN 344
Cdd:PRK06178 240 AAYAVAVVGGEDSVFLSF---LPEFWIAGENFGLLFPLFSGATLVLL-ARWDAVAFMAAVERYRVTRTVMLVDNAVELMD 315
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 345 NPDFQTLDFKPLILTlgggMAVQ------RPVAERWQHMTGCHITEG-YGLSET-------------------SPVACan 398
Cdd:PRK06178 316 HPRFAEYDLSSLRQV----RVVSfvkklnPDYRQRWRALTGSVLAEAaWGMTEThtcdtftagfqdddfdllsQPVFV-- 389
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 399 aldateftgtiGLPMPSTDVVIRDDDGSDLL-LGKVGEICVRGPQVMKGYWNRPEETARAIMaDGFFRTGDMGFMDERGY 477
Cdd:PRK06178 390 -----------GLPVPGTEFKICDFETGELLpLGAEGEIVVRTPSLLKGYWNKPEATAEALR-DGWLHTGDIGKIDEQGF 457
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 478 TKIVDRKKDMILVSGFNVYPNEIEEVAAEHPGVLESAAVGIPNEHSGEVVKLYVV-RRDPELTEDEVKAFCAERLTNYKR 556
Cdd:PRK06178 458 LHYLGRRKEMLKVNGMSVFPSEVEALLGQHPAVLGSAVVGRPDPDKGQVPVAFVQlKPGADLTAAALQAWCRENMAVYKV 537
|
570 580
....*....|....*....|...
gi 479816261 557 PrEVEFRESLPKSNVGKILRREL 579
Cdd:PRK06178 538 P-EIRIVDALPMTATGKVRKQDL 559
|
|
| PRK08315 |
PRK08315 |
AMP-binding domain protein; Validated |
33-581 |
7.94e-98 |
|
AMP-binding domain protein; Validated
Pssm-ID: 236236 [Multi-domain] Cd Length: 559 Bit Score: 308.66 E-value: 7.94e-98
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 33 LKSYPAGvPHDINLSAATsISDMISASCRQFANSPAFTCMGKDI--SYKELDDHSRALAAWLQSRGLVKGDRVAIMMPNI 110
Cdd:PRK08315 1 GLSYVRG-PTDVPLLEQT-IGQLLDRTAARYPDREALVYRDQGLrwTYREFNEEVDALAKGLLALGIEKGDRVGIWAPNV 78
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 111 LQYPITFTAVLRAGFVVVNVNPLYTPRELEHQLNDAGAKALVVLENF----------------ATTVEKALPSINVPNI- 173
Cdd:PRK08315 79 PEWVLTQFATAKIGAILVTINPAYRLSELEYALNQSGCKALIAADGFkdsdyvamlyelapelATCEPGQLQSARLPELr 158
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 174 LVASMGD-----MLGFkghivnlvvrrvkkmvPAWNIPGHVRFKDALAQgRAKSFNPVpvqgcDIAFLQYTGGTTGISKG 248
Cdd:PRK08315 159 RVIFLGDekhpgMLNF----------------DELLALGRAVDDAELAA-RQATLDPD-----DPINIQYTSGTTGFPKG 216
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 249 AMLTHSNILAN----VEQMNlwmdvaFRNKGKpkalnfVC-ALPLYHIFaltvnamiGMKLGARNIL-------IPNPrd 316
Cdd:PRK08315 217 ATLTHRNILNNgyfiGEAMK------LTEEDR------LCiPVPLYHCF--------GMVLGNLACVthgatmvYPGE-- 274
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 317 ipSF--------VKELKKYPVHifpGLNTLFNGLMNNPDFQTLDFKplilTLGGG-MA-------VQRPVAERwQHMTGc 380
Cdd:PRK08315 275 --GFdplatlaaVEEERCTALY---GVPTMFIAELDHPDFARFDLS----SLRTGiMAgspcpieVMKRVIDK-MHMSE- 343
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 381 hITEGYGLSETSPVACANALDaTEF---TGTIGLPMPSTDVVIRD-DDGSDLLLGKVGEICVRGPQVMKGYWNRPEETAR 456
Cdd:PRK08315 344 -VTIAYGMTETSPVSTQTRTD-DPLekrVTTVGRALPHLEVKIVDpETGETVPRGEQGELCTRGYSVMKGYWNDPEKTAE 421
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 457 AIMADGFFRTGDMGFMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVAAEHPGVLESAAVGIPNEHSGEVVKLYVVRRDP 536
Cdd:PRK08315 422 AIDADGWMHTGDLAVMDEEGYVNIVGRIKDMIIRGGENIYPREIEEFLYTHPKIQDVQVVGVPDEKYGEEVCAWIILRPG 501
|
570 580 590 600
....*....|....*....|....*....|....*....|....*.
gi 479816261 537 E-LTEDEVKAFCAERLTNYKRPREVEFRESLPKSNVGKILRRELRD 581
Cdd:PRK08315 502 AtLTEEDVRDFCRGKIAHYKIPRYIRFVDEFPMTVTGKIQKFKMRE 547
|
|
| FACL_fum10p_like |
cd05926 |
Subfamily of fatty acid CoA ligase (FACL) similar to Fum10p of Gibberella moniliformis; FACL ... |
64-581 |
2.10e-95 |
|
Subfamily of fatty acid CoA ligase (FACL) similar to Fum10p of Gibberella moniliformis; FACL catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, followed by the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Fum10p is a fatty acid CoA ligase involved in the synthesis of fumonisin, a polyketide mycotoxin, in Gibberella moniliformis.
Pssm-ID: 341249 [Multi-domain] Cd Length: 493 Bit Score: 300.38 E-value: 2.10e-95
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 64 ANSPAF--TCMGKDISYKELDDHSRALAAWLQSRGLVKGDRVAIMMPNILQYPITFTAVLRAGFVVVNVNPLYTPRELEH 141
Cdd:cd05926 1 PDAPALvvPGSTPALTYADLAELVDDLARQLAALGIKKGDRVAIALPNGLEFVVAFLAAARAGAVVAPLNPAYKKAEFEF 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 142 QLNDAGAKALVVLENFATTVEKALPSinvpnilvasmgdmlgFKGHIVNLVVR--RVKKMVPAWNIPGHvrfkdaLAQGR 219
Cdd:cd05926 81 YLADLGSKLVLTPKGELGPASRAASK----------------LGLAILELALDvgVLIRAPSAESLSNL------LADKK 138
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 220 AKSFNPVPvQGCDIAFLQYTGGTTGISKGAMLTHSNILANVeqmnlwmdvafRNKGKPKALNF----VCALPLYHIFALt 295
Cdd:cd05926 139 NAKSEGVP-LPDDLALILHTSGTTGRPKGVPLTHRNLAASA-----------TNITNTYKLTPddrtLVVMPLFHVHGL- 205
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 296 VNAMIGMKLGARNILIPNPRDIPSFVKELKKYPVHIFPGLNTLFNGLMNNPDFQTLDFKPL---ILTLGGGMAVQrpVAE 372
Cdd:cd05926 206 VASLLSTLAAGGSVVLPPRFSASTFWPDVRDYNATWYTAVPTIHQILLNRPEPNPESPPPKlrfIRSCSASLPPA--VLE 283
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 373 RWQHMTGCHITEGYGLSETSPVACANALDATEF-TGTIGLPMpSTDVVIRDDDGSDLLLGKVGEICVRGPQVMKGYWNRP 451
Cdd:cd05926 284 ALEATFGAPVLEAYGMTEAAHQMTSNPLPPGPRkPGSVGKPV-GVEVRILDEDGEILPPGVVGEICLRGPNVTRGYLNNP 362
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 452 EETARAIMADGFFRTGDMGFMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVAAEHPGVLESAAVGIPNEHSGEVVKLYV 531
Cdd:cd05926 363 EANAEAAFKDGWFRTGDLGYLDADGYLFLTGRIKELINRGGEKISPLEVDGVLLSHPAVLEAVAFGVPDEKYGEEVAAAV 442
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|.
gi 479816261 532 VRR-DPELTEDEVKAFCAERLTNYKRPREVEFRESLPKSNVGKILRRELRD 581
Cdd:cd05926 443 VLReGASVTEEELRAFCRKHLAAFKVPKKVYFVDELPKTATGKIQRRKVAE 493
|
|
| FACL_DitJ_like |
cd05934 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
73-580 |
2.24e-92 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Members of this family include DitJ from Pseudomonas and similar proteins.
Pssm-ID: 341257 [Multi-domain] Cd Length: 422 Bit Score: 289.96 E-value: 2.24e-92
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 73 GKDISYKELDDHSRALAAWLQSRGLVKGDRVAIMMPNILQYPITFTAVLRAGFVVVNVNPLYTPRELEHQLNDAGAKALV 152
Cdd:cd05934 1 GRRWTYAELLRESARIAAALAALGIRPGDRVALMLDNCPEFLFAWFALAKLGAVLVPINTALRGDELAYIIDHSGAQLVV 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 153 vlenfattvekalpsinvpnilvasmgdmlgfkghivnlvvrrvkkmvpawnipghvrfkdalaqgraksfnpvpvqgCD 232
Cdd:cd05934 81 ------------------------------------------------------------------------------VD 82
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 233 IAFLQYTGGTTGISKGAMLTHSNILANVEQMNLWM-----DVAFrnkgkpkalnfvCALPLYHIFALTVNAMIGMKLGAR 307
Cdd:cd05934 83 PASILYTSGTTGPPKGVVITHANLTFAGYYSARRFglgedDVYL------------TVLPLFHINAQAVSVLAALSVGAT 150
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 308 NILIP--NPRdipSFVKELKKYPVHIFPGLNTLFNGLMNNPDFQTLDFKPLILTLGGGMAVQ--RPVAERWqhmtGCHIT 383
Cdd:cd05934 151 LVLLPrfSAS---RFWSDVRRYGATVTNYLGAMLSYLLAQPPSPDDRAHRLRAAYGAPNPPElhEEFEERF----GVRLL 223
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 384 EGYGLSETSpVACANALDATEFTGTIGLPMPSTDVVIRDDDGSDLLLGKVGEICVR---GPQVMKGYWNRPEETARAiMA 460
Cdd:cd05934 224 EGYGMTETI-VGVIGPRDEPRRPGSIGRPAPGYEVRIVDDDGQELPAGEPGELVIRglrGWGFFKGYYNMPEATAEA-MR 301
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 461 DGFFRTGDMGFMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVAAEHPGVLESAAVGIPNEHSGEVVKLYVVRRDPE-LT 539
Cdd:cd05934 302 NGWFHTGDLGYRDADGFFYFVDRKKDMIRRRGENISSAEVERAILRHPAVREAAVVAVPDEVGEDEVKAVVVLRPGEtLD 381
|
490 500 510 520
....*....|....*....|....*....|....*....|.
gi 479816261 540 EDEVKAFCAERLTNYKRPREVEFRESLPKSNVGKILRRELR 580
Cdd:cd05934 382 PEELFAFCEGQLAYFKVPRYIRFVDDLPKTPTEKVAKAQLR 422
|
|
| BCL_4HBCL |
cd05959 |
Benzoate CoA ligase (BCL) and 4-Hydroxybenzoate-Coenzyme A Ligase (4-HBA-CoA ligase); Benzoate ... |
64-580 |
1.41e-90 |
|
Benzoate CoA ligase (BCL) and 4-Hydroxybenzoate-Coenzyme A Ligase (4-HBA-CoA ligase); Benzoate CoA ligase and 4-hydroxybenzoate-coenzyme A ligase catalyze the first activating step for benzoate and 4-hydroxybenzoate catabolic pathways, respectively. Although these two enzymes share very high sequence homology, they have their own substrate preference. The reaction proceeds via a two-step process; the first ATP-dependent step forms the substrate-AMP intermediate, while the second step forms the acyl-CoA ester, releasing the AMP. Aromatic compounds represent the second most abundant class of organic carbon compounds after carbohydrates. Some bacteria can use benzoic acid or benzenoid compounds as the sole source of carbon and energy through degradation. Benzoate CoA ligase and 4-hydroxybenzoate-Coenzyme A ligase are key enzymes of this process.
Pssm-ID: 341269 [Multi-domain] Cd Length: 508 Bit Score: 288.11 E-value: 1.41e-90
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 64 ANSPAFTCMGKDISYKELDDHSRALAAWLQSRGLVKGDRVAIMMPNILQYPITFTAVLRAGFVVVNVNPLYTPRELEHQL 143
Cdd:cd05959 18 GDKTAFIDDAGSLTYAELEAEARRVAGALRALGVKREERVLLIMLDTVDFPTAFLGAIRAGIVPVPVNTLLTPDDYAYYL 97
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 144 NDAGAKALVVLENFATTVEKALPSINVpnilvasmgdmlgfkgHIVNLVVRRvkkmvPAWNIPGHVRFKDALAqGRAKSF 223
Cdd:cd05959 98 EDSRARVVVVSGELAPVLAAALTKSEH----------------TLVVLIVSG-----GAGPEAGALLLAELVA-AEAEQL 155
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 224 NPVPVQGCDIAFLQYTGGTTGISKGAMLTHSNILanveqmnlWMDVAF-RNKGKPKAlNFVC--ALPLYHIFALTvNAMI 300
Cdd:cd05959 156 KPAATHADDPAFWLYSSGSTGRPKGVVHLHADIY--------WTAELYaRNVLGIRE-DDVCfsAAKLFFAYGLG-NSLT 225
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 301 -GMKLGARNILIPNpRDIP-SFVKELKKYPVHIFPGLNTLFNGLMNNPDFQTLDFKPLILTLGGGMAVQRPVAERWQHMT 378
Cdd:cd05959 226 fPLSVGATTVLMPE-RPTPaAVFKRIRRYRPTVFFGVPTLYAAMLAAPNLPSRDLSSLRLCVSAGEALPAEVGERWKARF 304
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 379 GCHITEGYGLSETSPVACANALDATEFtGTIGLPMPSTDVVIRDDDGSDLLLGKVGEICVRGPQVMKGYWNRPEETaRAI 458
Cdd:cd05959 305 GLDILDGIGSTEMLHIFLSNRPGRVRY-GTTGKPVPGYEVELRDEDGGDVADGEPGELYVRGPSSATMYWNNRDKT-RDT 382
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 459 MADGFFRTGDMGFMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVAAEHPGVLESAAVGIPNEHSGEVVKLYVVRR---- 534
Cdd:cd05959 383 FQGEWTRTGDKYVRDDDGFYTYAGRADDMLKVSGIWVSPFEVESALVQHPAVLEAAVVGVEDEDGLTKPKAFVVLRpgye 462
|
490 500 510 520
....*....|....*....|....*....|....*....|....*.
gi 479816261 535 DPELTEDEVKAFCAERLTNYKRPREVEFRESLPKSNVGKILRRELR 580
Cdd:cd05959 463 DSEALEEELKEFVKDRLAPYKYPRWIVFVDELPKTATGKIQRFKLR 508
|
|
| MCS |
cd05941 |
Malonyl-CoA synthetase (MCS); MCS catalyzes the formation of malonyl-CoA in a two-step ... |
65-581 |
3.11e-90 |
|
Malonyl-CoA synthetase (MCS); MCS catalyzes the formation of malonyl-CoA in a two-step reaction consisting of the adenylation of malonate with ATP, followed by malonyl transfer from malonyl-AMP to CoA. Malonic acid and its derivatives are the building blocks of polyketides and malonyl-CoA serves as the substrate of polyketide synthases. Malonyl-CoA synthetase has broad substrate tolerance and can activate a variety of malonyl acid derivatives. MCS may play an important role in biosynthesis of polyketides, the important secondary metabolites with therapeutic and agrochemical utility.
Pssm-ID: 341264 [Multi-domain] Cd Length: 442 Bit Score: 285.34 E-value: 3.11e-90
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 65 NSPAFTCMGKDISYKELDDHSRALA-AWLQSRGLVKGDRVAIMMPNILQYPITFTAVLRAGFVVVNVNPLYTPRELEHQL 143
Cdd:cd05941 1 DRIAIVDDGDSITYADLVARAARLAnRLLALGKDLRGDRVAFLAPPSAEYVVAQLAIWRAGGVAVPLNPSYPLAELEYVI 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 144 NDAGAKALVvlenfattvekalpsinvpnilvasmgdmlgfkghivnlvvrrvkkmvpawnipghvrfkdalaqgraksf 223
Cdd:cd05941 81 TDSEPSLVL----------------------------------------------------------------------- 89
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 224 npvpvqgcDIAFLQYTGGTTGISKGAMLTHSNILANVEQM-NLWmdvAFRNKGKpkalnFVCALPLYHIFALtVNAMIG- 301
Cdd:cd05941 90 --------DPALILYTSGTTGRPKGVVLTHANLAANVRALvDAW---RWTEDDV-----LLHVLPLHHVHGL-VNALLCp 152
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 302 MKLGARNILIPNPRDIPSFVKELKKyPVHIFPGLNTLFNGLMNNPDFQTLD--------FKPLILTLGGGMAVQRPVAER 373
Cdd:cd05941 153 LFAGASVEFLPKFDPKEVAISRLMP-SITVFMGVPTIYTRLLQYYEAHFTDpqfaraaaAERLRLMVSGSAALPVPTLEE 231
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 374 WQHMTGCHITEGYGLSETSpVACANALDATEFTGTIGLPMPSTDVVIRDDDGSDLLL-GKVGEICVRGPQVMKGYWNRPE 452
Cdd:cd05941 232 WEAITGHTLLERYGMTEIG-MALSNPLDGERRPGTVGMPLPGVQARIVDEETGEPLPrGEVGEIQVRGPSVFKEYWNKPE 310
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 453 ETARAIMADGFFRTGDMGFMDERGYTKIVDRKKDMILVS-GFNVYPNEIEEVAAEHPGVLESAAVGIPNEHSGEVVKLYV 531
Cdd:cd05941 311 ATKEEFTDDGWFKTGDLGVVDEDGYYWILGRSSVDIIKSgGYKVSALEIERVLLAHPGVSECAVIGVPDPDWGERVVAVV 390
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|..
gi 479816261 532 VRRD--PELTEDEVKAFCAERLTNYKRPREVEFRESLPKSNVGKILRRELRD 581
Cdd:cd05941 391 VLRAgaAALSLEELKEWAKQRLAPYKRPRRLILVDELPRNAMGKVNKKELRK 442
|
|
| FAA1 |
COG1022 |
Long-chain acyl-CoA synthetase (AMP-forming) [Lipid transport and metabolism]; |
46-517 |
1.33e-89 |
|
Long-chain acyl-CoA synthetase (AMP-forming) [Lipid transport and metabolism];
Pssm-ID: 440645 [Multi-domain] Cd Length: 603 Bit Score: 288.54 E-value: 1.33e-89
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 46 LSAATSISDMISASCRQFANSPAFTCMG----KDISYKELDDHSRALAAWLQSRGLVKGDRVAIMMPNILQYPITFTAVL 121
Cdd:COG1022 7 VPPADTLPDLLRRRAARFPDRVALREKEdgiwQSLTWAEFAERVRALAAGLLALGVKPGDRVAILSDNRPEWVIADLAIL 86
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 122 RAGFVVVnvnPLY---TPRELEHQLNDAGAKALVVlENfATTVEKALPSI-NVPNIlvasmgdmlgfkGHIVNLvvrrvk 197
Cdd:COG1022 87 AAGAVTV---PIYptsSAEEVAYILNDSGAKVLFV-ED-QEQLDKLLEVRdELPSL------------RHIVVL------ 143
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 198 KMVPAWNIPGHVRFKDALAQGRAKSFN------PVPVQGCDIAFLQYTGGTTGISKGAMLTHSNILANVEQMNLWMDVaf 271
Cdd:COG1022 144 DPRGLRDDPRLLSLDELLALGREVADPaelearRAAVKPDDLATIIYTSGTTGRPKGVMLTHRNLLSNARALLERLPL-- 221
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 272 rnKGKPKALNFvcaLPLYHIFALTVnAMIGMKLGARNILIPNPRDIPSFVKELKkyPvHIF------------------- 332
Cdd:COG1022 222 --GPGDRTLSF---LPLAHVFERTV-SYYALAAGATVAFAESPDTLAEDLREVK--P-TFMlavprvwekvyagiqakae 292
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 333 --PGL-NTLFN-------------------GLMNNPDFQTLD---FKPLILTLG--------GGMAVQRPVAeRWQHMTG 379
Cdd:COG1022 293 eaGGLkRKLFRwalavgrryararlagkspSLLLRLKHALADklvFSKLREALGgrlrfavsGGAALGPELA-RFFRALG 371
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 380 CHITEGYGLSETSPVACANALDATEFtGTIGLPMPSTDVVIRDDdgsdlllgkvGEICVRGPQVMKGYWNRPEETARAIM 459
Cdd:COG1022 372 IPVLEGYGLTETSPVITVNRPGDNRI-GTVGPPLPGVEVKIAED----------GEILVRGPNVMKGYYKNPEATAEAFD 440
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*....
gi 479816261 460 ADGFFRTGDMGFMDERGYTKIVDRKKDMI-LVSGFNVYPNEIEEVAAEHPGVLESAAVG 517
Cdd:COG1022 441 ADGWLHTGDIGELDEDGFLRITGRKKDLIvTSGGKNVAPQPIENALKASPLIEQAVVVG 499
|
|
| PRK12583 |
PRK12583 |
acyl-CoA synthetase; Provisional |
35-581 |
1.35e-89 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 237145 [Multi-domain] Cd Length: 558 Bit Score: 287.05 E-value: 1.35e-89
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 35 SYPAGVPhDINLSAATsISDMISASCRQFANSPA--FTCMGKDISYKELDDHSRALAAWLQSRGLVKGDRVAIMMPNILQ 112
Cdd:PRK12583 5 SYYQGGG-DKPLLTQT-IGDAFDATVARFPDREAlvVRHQALRYTWRQLADAVDRLARGLLALGVQPGDRVGIWAPNCAE 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 113 YPITFTAVLRAGFVVVNVNPLYTPRELEHQLNDAGAKALVVLENFATTVEKALPSINVPNILVASMGDMLGFKghIVNLv 192
Cdd:PRK12583 83 WLLTQFATARIGAILVNINPAYRASELEYALGQSGVRWVICADAFKTSDYHAMLQELLPGLAEGQPGALACER--LPEL- 159
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 193 vRRVKKMVPAwNIPGHVRFKDALAQGRAKSFNPV-----PVQGCDIAFLQYTGGTTGISKGAMLTHSNILANVeqmnlWM 267
Cdd:PRK12583 160 -RGVVSLAPA-PPPGFLAWHELQARGETVSREALaerqaSLDRDDPINIQYTSGTTGFPKGATLSHHNILNNG-----YF 232
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 268 dVAfRNKGKPKALNFVCALPLYHIFALTVNAMIGMKLGArNILIPN----PRDIPSFVKELKKYPVHifpGLNTLFNGLM 343
Cdd:PRK12583 233 -VA-ESLGLTEHDRLCVPVPLYHCFGMVLANLGCMTVGA-CLVYPNeafdPLATLQAVEEERCTALY---GVPTMFIAEL 306
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 344 NNPDFQTLDFKPLILTLGGG----MAVQRPVAERwQHMTgcHITEGYGLSETSPVA--CANALDATEFTGTIGLPMPSTD 417
Cdd:PRK12583 307 DHPQRGNFDLSSLRTGIMAGapcpIEVMRRVMDE-MHMA--EVQIAYGMTETSPVSlqTTAADDLERRVETVGRTQPHLE 383
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 418 VVIRDDDGSDLLLGKVGEICVRGPQVMKGYWNRPEETARAIMADGFFRTGDMGFMDERGYTKIVDRKKDMILVSGFNVYP 497
Cdd:PRK12583 384 VKVVDPDGATVPRGEIGELCTRGYSVMKGYWNNPEATAESIDEDGWMHTGDLATMDEQGYVRIVGRSKDMIIRGGENIYP 463
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 498 NEIEEVAAEHPGVLESAAVGIPNEHSGEVVKLYVV-RRDPELTEDEVKAFCAERLTNYKRPREVEFRESLPKSNVGKILR 576
Cdd:PRK12583 464 REIEEFLFTHPAVADVQVFGVPDEKYGEEIVAWVRlHPGHAASEEELREFCKARIAHFKVPRYFRFVDEFPMTVTGKVQK 543
|
....*
gi 479816261 577 RELRD 581
Cdd:PRK12583 544 FRMRE 548
|
|
| PRK07514 |
PRK07514 |
malonyl-CoA synthase; Validated |
73-581 |
3.47e-85 |
|
malonyl-CoA synthase; Validated
Pssm-ID: 181011 [Multi-domain] Cd Length: 504 Bit Score: 274.06 E-value: 3.47e-85
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 73 GKDISYKELDDHSRALAAWLQSRGLVKGDRVAIMMPNILQYPITFTAVLRAGFVVVNVNPLYTPRELEHQLNDAGAKALV 152
Cdd:PRK07514 26 GLRYTYGDLDAASARLANLLVALGVKPGDRVAVQVEKSPEALALYLATLRAGAVFLPLNTAYTLAELDYFIGDAEPALVV 105
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 153 VLENFATTVEKALPSINVPNILVasmgdmLGFKGHIVNLvvrrvkkmvpawnipghvrfkdALAQGRAKSFNPVPVQGCD 232
Cdd:PRK07514 106 CDPANFAWLSKIAAAAGAPHVET------LDADGTGSLL----------------------EAAAAAPDDFETVPRGADD 157
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 233 IAFLQYTGGTTGISKGAMLTHSNILANVEQM-NLWmdvAFRNKGKpkalnFVCALPLYHIFALTVNAMIGMKLGARNILI 311
Cdd:PRK07514 158 LAAILYTSGTTGRSKGAMLSHGNLLSNALTLvDYW---RFTPDDV-----LIHALPIFHTHGLFVATNVALLAGASMIFL 229
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 312 P--NPRDIpsfVKELKKYPVhiFPGLNTLFNGLMNNPDFQTLDFKPLILTLGGGMAVQRPVAERWQHMTGCHITEGYGLS 389
Cdd:PRK07514 230 PkfDPDAV---LALMPRATV--MMGVPTFYTRLLQEPRLTREAAAHMRLFISGSAPLLAETHREFQERTGHAILERYGMT 304
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 390 ETSpVACANALDATEFTGTIGLPMPSTDVVIRD-DDGSDLLLGKVGEICVRGPQVMKGYWNRPEETARAIMADGFFRTGD 468
Cdd:PRK07514 305 ETN-MNTSNPYDGERRAGTVGFPLPGVSLRVTDpETGAELPPGEIGMIEVKGPNVFKGYWRMPEKTAEEFRADGFFITGD 383
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 469 MGFMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVAAEHPGVLESAAVGIPNEHSGEVVKLYVVRR-DPELTEDEVKAFC 547
Cdd:PRK07514 384 LGKIDERGYVHIVGRGKDLIISGGYNVYPKEVEGEIDELPGVVESAVIGVPHPDFGEGVTAVVVPKpGAALDEAAILAAL 463
|
490 500 510
....*....|....*....|....*....|....
gi 479816261 548 AERLTNYKRPREVEFRESLPKSNVGKILRRELRD 581
Cdd:PRK07514 464 KGRLARFKQPKRVFFVDELPRNTMGKVQKNLLRE 497
|
|
| PRK06839 |
PRK06839 |
o-succinylbenzoate--CoA ligase; |
76-579 |
3.96e-84 |
|
o-succinylbenzoate--CoA ligase;
Pssm-ID: 168698 [Multi-domain] Cd Length: 496 Bit Score: 270.96 E-value: 3.96e-84
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 76 ISYKELDDHSRALAAWLQSR-GLVKGDRVAIMMPNILQYPITFTAVLRAGFVVVNVNPLYTPRELEHQLNDAGAKALVVL 154
Cdd:PRK06839 28 MTYKQLHEYVSKVAAYLIYElNVKKGERIAILSQNSLEYIVLLFAIAKVECIAVPLNIRLTENELIFQLKDSGTTVLFVE 107
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 155 ENFATTVEKALPSINVPNIlvasmgdmlgfkghivnlvvrrvkkmvpawnipghVRFKDALAQGRAKSFNPVPVQGCDIA 234
Cdd:PRK06839 108 KTFQNMALSMQKVSYVQRV-----------------------------------ISITSLKEIEDRKIDNFVEKNESASF 152
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 235 FLQYTGGTTGISKGAMLTHSNILANVEQMNLWMDVAFRNKGkpkalnfVCALPLYHIFALTVNAMIGMKLGARnILIPNP 314
Cdd:PRK06839 153 IICYTSGTTGKPKGAVLTQENMFWNALNNTFAIDLTMHDRS-------IVLLPLFHIGGIGLFAFPTLFAGGV-IIVPRK 224
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 315 RDIPSFVKELKKYPVHIFPGLNTLFNGLMNNPDFQTLDFKPLILTLGGGMAVQRPVAERWQHmTGCHITEGYGLSETSP- 393
Cdd:PRK06839 225 FEPTKALSMIEKHKVTVVMGVPTIHQALINCSKFETTNLQSVRWFYNGGAPCPEELMREFID-RGFLFGQGFGMTETSPt 303
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 394 VACANALDATEFTGTIGLPMPSTDVVIRDDDGSDLLLGKVGEICVRGPQVMKGYWNRPEETARAIMaDGFFRTGDMGFMD 473
Cdd:PRK06839 304 VFMLSEEDARRKVGSIGKPVLFCDYELIDENKNKVEVGEVGELLIRGPNVMKEYWNRPDATEETIQ-DGWLCTGDLARVD 382
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 474 ERGYTKIVDRKKDMILVSGFNVYPNEIEEVAAEHPGVLESAAVGIPNEHSGEVVKLYVVRRD-PELTEDEVKAFCAERLT 552
Cdd:PRK06839 383 EDGFVYIVGRKKEMIISGGENIYPLEVEQVINKLSDVYEVAVVGRQHVKWGEIPIAFIVKKSsSVLIEKDVIEHCRLFLA 462
|
490 500
....*....|....*....|....*..
gi 479816261 553 NYKRPREVEFRESLPKSNVGKILRREL 579
Cdd:PRK06839 463 KYKIPKEIVFLKELPKNATGKIQKAQL 489
|
|
| MACS_like |
cd05972 |
Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step activation of ... |
77-581 |
1.17e-83 |
|
Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. The acyl-CoA is a key intermediate in many important biosynthetic and catabolic processes.
Pssm-ID: 341276 [Multi-domain] Cd Length: 428 Bit Score: 267.67 E-value: 1.17e-83
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 77 SYKELDDHSRALAAWLQSRGLVKGDRVAIMMPNILQYPITFTAVLRAGFVVVNVNPLYTPRELEHQLNDAGAKALVVLEN 156
Cdd:cd05972 2 SFRELKRESAKAANVLAKLGLRKGDRVAVLLPRVPELWAVILAVIKLGAVYVPLTTLLGPKDIEYRLEAAGAKAIVTDAE 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 157 fattvekalpsinvpnilvasmgdmlgfkghivnlvvrrvkkmvpawnipghvrfkdalaqgraksfnpvpvqgcDIAFL 236
Cdd:cd05972 82 ---------------------------------------------------------------------------DPALI 86
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 237 QYTGGTTGISKGAMLTHSNILANVEQMNLWMDVafrnkgKPKALNFVCALPLYHIFALTvnAMIG-MKLGARNILIPNPR 315
Cdd:cd05972 87 YFTSGTTGLPKGVLHTHSYPLGHIPTAAYWLGL------RPDDIHWNIADPGWAKGAWS--SFFGpWLLGATVFVYEGPR 158
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 316 DIPS-FVKELKKYPVHIFPGLNTLFNgLMNNPDFQTLDFKPLILTLGGGMAVQRPVAERWQHMTGCHITEGYGLSETSpV 394
Cdd:cd05972 159 FDAErILELLERYGVTSFCGPPTAYR-MLIKQDLSSYKFSHLRLVVSAGEPLNPEVIEWWRAATGLPIRDGYGQTETG-L 236
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 395 ACANALDATEFTGTIGLPMPSTDVVIRDDDGSDLLLGKVGEICVR--GPQVMKGYWNRPEETARAImADGFFRTGDMGFM 472
Cdd:cd05972 237 TVGNFPDMPVKPGSMGRPTPGYDVAIIDDDGRELPPGEEGDIAIKlpPPGLFLGYVGDPEKTEASI-RGDYYLTGDRAYR 315
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 473 DERGYTKIVDRKKDMILVSGFNVYPNEIEEVAAEHPGVLESAAVGIPNEHSGEVVKLYVVRRD-----PELTEdEVKAFC 547
Cdd:cd05972 316 DEDGYFWFVGRADDIIKSSGYRIGPFEVESALLEHPAVAEAAVVGSPDPVRGEVVKAFVVLTSgyepsEELAE-ELQGHV 394
|
490 500 510
....*....|....*....|....*....|....
gi 479816261 548 AERLTNYKRPREVEFRESLPKSNVGKILRRELRD 581
Cdd:cd05972 395 KKVLAPYKYPREIEFVEELPKTISGKIRRVELRD 428
|
|
| PLN02246 |
PLN02246 |
4-coumarate--CoA ligase |
43-580 |
1.37e-82 |
|
4-coumarate--CoA ligase
Pssm-ID: 215137 [Multi-domain] Cd Length: 537 Bit Score: 268.39 E-value: 1.37e-82
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 43 DINLSAATSISDMISASCRQFANSPaftCM-----GKDISYKELDDHSRALAAWLQSRGLVKGDRVAIMMPNILQYPITF 117
Cdd:PLN02246 16 DIYIPNHLPLHDYCFERLSEFSDRP---CLidgatGRVYTYADVELLSRRVAAGLHKLGIRQGDVVMLLLPNCPEFVLAF 92
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 118 TAVLRAGFVVVNVNPLYTPRELEHQLNDAGAKaLVVLEnfATTVEKALPSINVPNILVASMGDmlgfkghivnlvvrrvk 197
Cdd:PLN02246 93 LGASRRGAVTTTANPFYTPAEIAKQAKASGAK-LIITQ--SCYVDKLKGLAEDDGVTVVTIDD----------------- 152
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 198 kmvpawNIPGHVRFKDaLAQGRAKSFNPVPVQGCDIAFLQYTGGTTGISKGAMLTHSNILANVEQM------NLWM---D 268
Cdd:PLN02246 153 ------PPEGCLHFSE-LTQADENELPEVEISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQvdgenpNLYFhsdD 225
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 269 VAfrnkgkpkalnfVCALPLYHIFALTVNAMIGMKLGArNILIPNPRDIPSFVKELKKYPVHIFPGLNTLFNGLMNNPDF 348
Cdd:PLN02246 226 VI------------LCVLPMFHIYSLNSVLLCGLRVGA-AILIMPKFEIGALLELIQRHKVTIAPFVPPIVLAIAKSPVV 292
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 349 QTLDFKPLILTLGG----GMAVQRPVAERwqhMTGCHITEGYGLSETSPV-ACANALDATEF---TGTIGLPMPSTDVVI 420
Cdd:PLN02246 293 EKYDLSSIRMVLSGaaplGKELEDAFRAK---LPNAVLGQGYGMTEAGPVlAMCLAFAKEPFpvkSGSCGTVVRNAELKI 369
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 421 RD-DDGSDLLLGKVGEICVRGPQVMKGYWNRPEETARAIMADGFFRTGDMGFMDERGYTKIVDRKKDMILVSGFNVYPNE 499
Cdd:PLN02246 370 VDpETGASLPRNQPGEICIRGPQIMKGYLNDPEATANTIDKDGWLHTGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAE 449
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 500 IEEVAAEHPGVLESAAVGIPNEHSGEVVKLYVVRR-DPELTEDEVKAFCAERLTNYKRPREVEFRESLPKSNVGKILRRE 578
Cdd:PLN02246 450 LEALLISHPSIADAAVVPMKDEVAGEVPVAFVVRSnGSEITEDEIKQFVAKQVVFYKRIHKVFFVDSIPKAPSGKILRKD 529
|
..
gi 479816261 579 LR 580
Cdd:PLN02246 530 LR 531
|
|
| EntE |
COG1021 |
EntE, 2,3-dihydroxybenzoate-AMP synthase component of non-ribosomal peptide synthetase ... |
52-580 |
3.26e-82 |
|
EntE, 2,3-dihydroxybenzoate-AMP synthase component of non-ribosomal peptide synthetase [Secondary metabolites biosynthesis, transport and catabolism];
Pssm-ID: 440644 [Multi-domain] Cd Length: 533 Bit Score: 267.01 E-value: 3.26e-82
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 52 ISDMISASCRQFANSPAFTCMGKDISYKELDDHSRALAAWLQSRGLVKGDRVAIMMPNILQYPITFTAVLRAGFVVVNVN 131
Cdd:COG1021 27 LGDLLRRRAERHPDRIAVVDGERRLSYAELDRRADRLAAGLLALGLRPGDRVVVQLPNVAEFVIVFFALFRAGAIPVFAL 106
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 132 PLYTPRELEHQLNDAGAKALVV--------LENFATTVEKALPSINVPnILVASMGDMLGFkghivnlvvrrvkkmvpaw 203
Cdd:COG1021 107 PAHRRAEISHFAEQSEAVAYIIpdrhrgfdYRALARELQAEVPSLRHV-LVVGDAGEFTSL------------------- 166
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 204 nipghvrfkDALAQGRAKSFNPVPvQGCDIAFLQYTGGTTGISKGAMLTHS----NILANVEQMNLWMDVAFrnkgkpka 279
Cdd:COG1021 167 ---------DALLAAPADLSEPRP-DPDDVAFFQLSGGTTGLPKLIPRTHDdylySVRASAEICGLDADTVY-------- 228
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 280 lnfVCALPLYHIFALTVNAMIG-MKLGARNILIPNPRdipsfvkelkkyPVHIFP-----GLN------TLFNGLMNNPD 347
Cdd:COG1021 229 ---LAALPAAHNFPLSSPGVLGvLYAGGTVVLAPDPS------------PDTAFPliereRVTvtalvpPLALLWLDAAE 293
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 348 FQTLDFKPLILTLGGGMAVQRPVAERWQHMTGCHITEGYGLSEtSPVaCANALDATEFT--GTIGLPMPSTD-VVIRDDD 424
Cdd:COG1021 294 RSRYDLSSLRVLQVGGAKLSPELARRVRPALGCTLQQVFGMAE-GLV-NYTRLDDPEEVilTTQGRPISPDDeVRIVDED 371
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 425 GSDLLLGKVGEICVRGPQVMKGYWNRPEETARAIMADGFFRTGDMGFMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVA 504
Cdd:COG1021 372 GNPVPPGEVGELLTRGPYTIRGYYRAPEHNARAFTPDGFYRTGDLVRRTPDGYLVVEGRAKDQINRGGEKIAAEEVENLL 451
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 479816261 505 AEHPGVLESAAVGIPNEHSGEVVKLYVVRRDPELTEDEVKAFCAER-LTNYKRPREVEFRESLPKSNVGKILRRELR 580
Cdd:COG1021 452 LAHPAVHDAAVVAMPDEYLGERSCAFVVPRGEPLTLAELRRFLRERgLAAFKLPDRLEFVDALPLTAVGKIDKKALR 528
|
|
| PRK06188 |
PRK06188 |
acyl-CoA synthetase; Validated |
46-580 |
3.63e-81 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 235731 [Multi-domain] Cd Length: 524 Bit Score: 264.16 E-value: 3.63e-81
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 46 LSAATSISDMISASCRQFANSPAFTCMGKDISYKELDDH-SRALAAWlQSRGLVKGDRVAIMMPNILQYPITFTAVLRAG 124
Cdd:PRK06188 8 LHSGATYGHLLVSALKRYPDRPALVLGDTRLTYGQLADRiSRYIQAF-EALGLGTGDAVALLSLNRPEVLMAIGAAQLAG 86
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 125 FVVVNVNPLYTPRELEHQLNDAGAKALVVleNFATTVEKALpsinvpnILVASmgdmlgfkghivnlvVRRVKKMVPAWN 204
Cdd:PRK06188 87 LRRTALHPLGSLDDHAYVLEDAGISTLIV--DPAPFVERAL-------ALLAR---------------VPSLKHVLTLGP 142
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 205 IPGHVrfkDALAQgrAKSFNPVPV----QGCDIAFLQYTGGTTGISKGAMLTH-SNILANVEQMNLWmdvafrnkGKPKA 279
Cdd:PRK06188 143 VPDGV---DLLAA--AAKFGPAPLvaaaLPPDIAGLAYTGGTTGKPKGVMGTHrSIATMAQIQLAEW--------EWPAD 209
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 280 LNFVCALPLYHIFALTVnaMIGMKLGARNILIP--NPRDIPSFVKELKKYPVHIFPglnTLFNGLMNNPDFQTLDFKPLI 357
Cdd:PRK06188 210 PRFLMCTPLSHAGGAFF--LPTLLRGGTVIVLAkfDPAEVLRAIEEQRITATFLVP---TMIYALLDHPDLRTRDLSSLE 284
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 358 LTLGGGMAvqrpvaerwqhMTGCHITEG-----------YGLSETSPVAC-----ANALDATEFTGTIGLPMPSTDVVIR 421
Cdd:PRK06188 285 TVYYGASP-----------MSPVRLAEAierfgpifaqyYGQTEAPMVITylrkrDHDPDDPKRLTSCGRPTPGLRVALL 353
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 422 DDDGSDLLLGKVGEICVRGPQVMKGYWNRPEETARAiMADGFFRTGDMGFMDERGYTKIVDRKKDMILVSGFNVYPNEIE 501
Cdd:PRK06188 354 DEDGREVAQGEVGEICVRGPLVMDGYWNRPEETAEA-FRDGWLHTGDVAREDEDGFYYIVDRKKDMIVTGGFNVFPREVE 432
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 502 EVAAEHPGVLESAAVGIPNEHSGEVVKLYVVRR-DPELTEDEVKAFCAERLTNYKRPREVEFRESLPKSNVGKILRRELR 580
Cdd:PRK06188 433 DVLAEHPAVAQVAVIGVPDEKWGEAVTAVVVLRpGAAVDAAELQAHVKERKGSVHAPKQVDFVDSLPLTALGKPDKKALR 512
|
|
| PRK07529 |
PRK07529 |
AMP-binding domain protein; Validated |
48-580 |
1.55e-80 |
|
AMP-binding domain protein; Validated
Pssm-ID: 236043 [Multi-domain] Cd Length: 632 Bit Score: 265.28 E-value: 1.55e-80
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 48 AATSISDMISASCRQFANSPAFTC--------MGKDISYKEL-DDHSRAlAAWLQSRGLVKGDRVAIMMPNILQYPITFT 118
Cdd:PRK07529 23 LPASTYELLSRAAARHPDAPALSFlldadpldRPETWTYAELlADVTRT-ANLLHSLGVGPGDVVAFLLPNLPETHFALW 101
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 119 AVLRAGfVVVNVNPLYTPRELEHQLNDAGAKALVVLENFATT-----VEKALPSinVPN----ILVASMGDMLGFKGHIV 189
Cdd:PRK07529 102 GGEAAG-IANPINPLLEPEQIAELLRAAGAKVLVTLGPFPGTdiwqkVAEVLAA--LPElrtvVEVDLARYLPGPKRLAV 178
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 190 NLVVRrvkkmvpawniPGHVR---FKDALAQGRA-KSFNPVPVQGCDIAFLQYTGGTTGISKGAMLTHSNILANVEQMNL 265
Cdd:PRK07529 179 PLIRR-----------KAHARildFDAELARQPGdRLFSGRPIGPDDVAAYFHTGGTTGMPKLAQHTHGNEVANAWLGAL 247
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 266 wmdVAFRNKGKpkalNFVCALPLYHIFALTVNAMIGMKLGArNILIPNP---RD---IPSFVKELKKYPVHIFPGLNTLF 339
Cdd:PRK07529 248 ---LLGLGPGD----TVFCGLPLFHVNALLVTGLAPLARGA-HVVLATPqgyRGpgvIANFWKIVERYRINFLSGVPTVY 319
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 340 NGLMNNP----DFQTLDFkplilTLGGGMAVQRPVAERWQHMTGCHITEGYGLSETSPVACANALDATEFTGTIGLPMPS 415
Cdd:PRK07529 320 AALLQVPvdghDISSLRY-----ALCGAAPLPVEVFRRFEAATGVRIVEGYGLTEATCVSSVNPPDGERRIGSVGLRLPY 394
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 416 TDV--VIRDDDG---SDLLLGKVGEICVRGPQVMKGYWNrPEETARAIMADGFFRTGDMGFMDERGYTKIVDRKKDMILV 490
Cdd:PRK07529 395 QRVrvVILDDAGrylRDCAVDEVGVLCIAGPNVFSGYLE-AAHNKGLWLEDGWLNTGDLGRIDADGYFWLTGRAKDLIIR 473
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 491 SGFNVYPNEIEEVAAEHPGVLESAAVGIPNEHSGEVVKLYV-VRRDPELTEDEVKAFCAERLTnyKR---PREVEFRESL 566
Cdd:PRK07529 474 GGHNIDPAAIEEALLRHPAVALAAAVGRPDAHAGELPVAYVqLKPGASATEAELLAFARDHIA--ERaavPKHVRILDAL 551
|
570
....*....|....
gi 479816261 567 PKSNVGKILRRELR 580
Cdd:PRK07529 552 PKTAVGKIFKPALR 565
|
|
| FACL_like_2 |
cd05917 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
236-580 |
2.66e-80 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341241 [Multi-domain] Cd Length: 349 Bit Score: 256.44 E-value: 2.66e-80
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 236 LQYTGGTTGISKGAMLTHSNILANVEQMNLWMDvaFRNKGKpkalnFVCALPLYHIFALTVNAMIGMKLGARNILIP--- 312
Cdd:cd05917 7 IQFTSGTTGSPKGATLTHHNIVNNGYFIGERLG--LTEQDR-----LCIPVPLFHCFGSVLGVLACLTHGATMVFPSpsf 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 313 NPRDIPSFVKELKKYPVHifpGLNTLFNGLMNNPDFQTLDFKPLILTLGGGMAVQRPVAERWQ---HMTGCHIteGYGLS 389
Cdd:cd05917 80 DPLAVLEAIEKEKCTALH---GVPTMFIAELEHPDFDKFDLSSLRTGIMAGAPCPPELMKRVIevmNMKDVTI--AYGMT 154
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 390 ETSPVACANALDATEFT--GTIGLPMPSTDVVIRD-DDGSDLLLGKVGEICVRGPQVMKGYWNRPEETARAIMADGFFRT 466
Cdd:cd05917 155 ETSPVSTQTRTDDSIEKrvNTVGRIMPHTEAKIVDpEGGIVPPVGVPGELCIRGYSVMKGYWNDPEKTAEAIDGDGWLHT 234
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 467 GDMGFMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVAAEHPGVLESAAVGIPNEHSGEVVKLYVVRRD-PELTEDEVKA 545
Cdd:cd05917 235 GDLAVMDEDGYCRIVGRIKDMIIRGGENIYPREIEEFLHTHPKVSDVQVVGVPDERYGEEVCAWIRLKEgAELTEEDIKA 314
|
330 340 350
....*....|....*....|....*....|....*
gi 479816261 546 FCAERLTNYKRPREVEFRESLPKSNVGKILRRELR 580
Cdd:cd05917 315 YCKGKIAHYKVPRYVFFVDEFPLTVSGKIQKFKLR 349
|
|
| PRK06087 |
PRK06087 |
medium-chain fatty-acid--CoA ligase; |
50-580 |
1.23e-79 |
|
medium-chain fatty-acid--CoA ligase;
Pssm-ID: 180393 [Multi-domain] Cd Length: 547 Bit Score: 260.84 E-value: 1.23e-79
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 50 TSISDMISASCRQFANSPAFT-CMGKDISYKELDDHSRALAAWLQSRGLVKGDRVAIMMPNILQYPITFTAVLRAGFVVV 128
Cdd:PRK06087 23 ASLADYWQQTARAMPDKIAVVdNHGASYTYSALDHAASRLANWLLAKGIEPGDRVAFQLPGWCEFTIIYLACLKVGAVSV 102
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 129 NVNPLYTPRELEHQLNDAGAKALvvlenFATTVEKalpSINvPNILVASMGDMLGFKGHIVNlvvrrVKKMVPAWNipgh 208
Cdd:PRK06087 103 PLLPSWREAELVWVLNKCQAKMF-----FAPTLFK---QTR-PVDLILPLQNQLPQLQQIVG-----VDKLAPATS---- 164
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 209 vrfKDALAQGRAKSF---NPVPVQGCDIAFLQYTGGTTGISKGAMLTHSNILAN----VEQMNL-WMDVAFRnkgkPKAL 280
Cdd:PRK06087 165 ---SLSLSQIIADYEpltTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASerayCARLNLtWQDVFMM----PAPL 237
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 281 NFvcALPLYHIFALTvnamigMKLGARNIL--IPNPRDIPSFVKELKKYPVHifpGLNTLFNGLMNNPDFQTLDFKPLIL 358
Cdd:PRK06087 238 GH--ATGFLHGVTAP------FLIGARSVLldIFTPDACLALLEQQRCTCML---GATPFIYDLLNLLEKQPADLSALRF 306
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 359 TLGGGMAVQRPVAER-WQHmtGCHITEGYGLSETSPVACANALDATEFTG-TIGLPMPSTDVVIRDDDGSDLLLGKVGEI 436
Cdd:PRK06087 307 FLCGGTTIPKKVAREcQQR--GIKLLSVYGSTESSPHAVVNLDDPLSRFMhTDGYAAAGVEIKVVDEARKTLPPGCEGEE 384
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 437 CVRGPQVMKGYWNRPEETARAIMADGFFRTGDMGFMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVAAEHPGVLESAAV 516
Cdd:PRK06087 385 ASRGPNVFMGYLDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVEDILLQHPKIHDACVV 464
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 479816261 517 GIPNEHSGEVVKLYVVRRDPELT---EDEVKAFCAERLTNYKRPREVEFRESLPKSNVGKILRRELR 580
Cdd:PRK06087 465 AMPDERLGERSCAYVVLKAPHHSltlEEVVAFFSRKRVAKYKYPEHIVVIDKLPRTASGKIQKFLLR 531
|
|
| PRK07786 |
PRK07786 |
long-chain-fatty-acid--CoA ligase; Validated |
65-580 |
3.66e-76 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 169098 [Multi-domain] Cd Length: 542 Bit Score: 251.62 E-value: 3.66e-76
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 65 NSPAFTCMGKDISYKELDDHSRALAAWLQSRGLVKGDRVAIMMPNILQYPITFTAVLRAGFVVVNVNPLYTPRELEHQLN 144
Cdd:PRK07786 32 DAPALRFLGNTTTWRELDDRVAALAGALSRRGVGFGDRVLILMLNRTEFVESVLAANMLGAIAVPVNFRLTPPEIAFLVS 111
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 145 DAGAKALV---VLENFATTVEKALPSIN-VPNILVASMGDMLGFKghivnlvvrrvkkmvpawnipghvrfkDALAQGRA 220
Cdd:PRK07786 112 DCGAHVVVteaALAPVATAVRDIVPLLStVVVAGGSSDDSVLGYE---------------------------DLLAEAGP 164
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 221 kSFNPVPVQGCDIAFLQYTGGTTGISKGAMLTHSNILANVeQMNLWMDVAFRNKgkpkALNFvCALPLYHIFALTvNAMI 300
Cdd:PRK07786 165 -AHAPVDIPNDSPALIMYTSGTTGRPKGAVLTHANLTGQA-MTCLRTNGADINS----DVGF-VGVPLFHIAGIG-SMLP 236
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 301 GMKLGARNILIP----NPRDIPSFVKELKKYPVHIFPglnTLFNGLMNNPDFQTLDFKPLILTLGGGMAVQRPVAERWQH 376
Cdd:PRK07786 237 GLLLGAPTVIYPlgafDPGQLLDVLEAEKVTGIFLVP---AQWQAVCAEQQARPRDLALRVLSWGAAPASDTLLRQMAAT 313
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 377 MTGCHITEGYGLSETSPVACA-NALDATEFTGTIGLPMPSTDVVIRDDDGSDLLLGKVGEICVRGPQVMKGYWNRPEETA 455
Cdd:PRK07786 314 FPEAQILAAFGQTEMSPVTCMlLGEDAIRKLGSVGKVIPTVAARVVDENMNDVPVGEVGEIVYRAPTLMSGYWNNPEATA 393
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 456 RAiMADGFFRTGDMGFMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVAAEHPGVLESAAVGIPNEHSGEVVKLYVVRRD 535
Cdd:PRK07786 394 EA-FAGGWFHSGDLVRQDEEGYVWVVDRKKDMIISGGENIYCAEVENVLASHPDIVEVAVIGRADEKWGEVPVAVAAVRN 472
|
490 500 510 520
....*....|....*....|....*....|....*....|....*..
gi 479816261 536 P--ELTEDEVKAFCAERLTNYKRPREVEFRESLPKSNVGKILRRELR 580
Cdd:PRK07786 473 DdaALTLEDLAEFLTDRLARYKHPKALEIVDALPRNPAGKVLKTELR 519
|
|
| OSB_CoA_lg |
cd05912 |
O-succinylbenzoate-CoA ligase (also known as O-succinylbenzoate-CoA synthase, OSB-CoA ... |
75-581 |
3.72e-76 |
|
O-succinylbenzoate-CoA ligase (also known as O-succinylbenzoate-CoA synthase, OSB-CoA synthetase, or MenE); O-succinylbenzoic acid-CoA synthase catalyzes the coenzyme A (CoA)- and ATP-dependent conversion of o-succinylbenzoic acid to o-succinylbenzoyl-CoA. The reaction is the fourth step of the biosynthesis pathway of menaquinone (vitamin K2). In certain bacteria, menaquinone is used during fumarate reduction in anaerobic respiration. In cyanobacteria, the product of the menaquinone pathway is phylloquinone (2-methyl-3-phytyl-1,4-naphthoquinone), a molecule used exclusively as an electron transfer cofactor in Photosystem 1. In green sulfur bacteria and heliobacteria, menaquinones are used as loosely bound secondary electron acceptors in the photosynthetic reaction center.
Pssm-ID: 341238 [Multi-domain] Cd Length: 411 Bit Score: 247.65 E-value: 3.72e-76
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 75 DISYKELDDHSRALAAWLQSRGLVKGDRVAIMMPNILQYPITFTAVLRAGFVVVNVNPLYTPRELEHQLNDAGAKalvvl 154
Cdd:cd05912 1 SYTFAELFEEVSRLAEHLAALGVRKGDRVALLSKNSIEMILLIHALWLLGAEAVLLNTRLTPNELAFQLKDSDVK----- 75
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 155 enfattvekalpsinvpnilvasmgdmlgfkghivnlvvrrvkkmvpawnipghvrfKDalaqgraksfnpvpvqgcDIA 234
Cdd:cd05912 76 ---------------------------------------------------------LD------------------DIA 80
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 235 FLQYTGGTTGISKGAMLTHSNILANV--EQMNLWMDvafRNKgkpkalNFVCALPLYHIFALTVnAMIGMKLGARNILIP 312
Cdd:cd05912 81 TIMYTSGTTGKPKGVQQTFGNHWWSAigSALNLGLT---EDD------NWLCALPLFHISGLSI-LMRSVIYGMTVYLVD 150
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 313 --NPRDIPSFVKELKkypVHIFPGLNTLFNGLMN------NPDFQTldfkplILtLGGGMAVQrPVAERWQHMtGCHITE 384
Cdd:cd05912 151 kfDAEQVLHLINSGK---VTIISVVPTMLQRLLEilgegyPNNLRC------IL-LGGGPAPK-PLLEQCKEK-GIPVYQ 218
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 385 GYGLSET-SPVACANALDATEFTGTIGLPMPSTDVVIRDDDGSdllLGKVGEICVRGPQVMKGYWNRPEETARAImADGF 463
Cdd:cd05912 219 SYGMTETcSQIVTLSPEDALNKIGSAGKPLFPVELKIEDDGQP---PYEVGEILLKGPNVTKGYLNRPDATEESF-ENGW 294
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 464 FRTGDMGFMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVAAEHPGVLESAAVGIPNEHSGEVVKLYVVrRDPELTEDEV 543
Cdd:cd05912 295 FKTGDIGYLDEEGFLYVLDRRSDLIISGGENIYPAEIEEVLLSHPAIKEAGVVGIPDDKWGQVPVAFVV-SERPISEEEL 373
|
490 500 510
....*....|....*....|....*....|....*...
gi 479816261 544 KAFCAERLTNYKRPREVEFRESLPKSNVGKILRRELRD 581
Cdd:cd05912 374 IAYCSEKLAKYKVPKKIYFVDELPRTASGKLLRHELKQ 411
|
|
| CHC_CoA_lg |
cd05903 |
Cyclohexanecarboxylate-CoA ligase (also called cyclohex-1-ene-1-carboxylate:CoA ligase); ... |
77-580 |
5.74e-76 |
|
Cyclohexanecarboxylate-CoA ligase (also called cyclohex-1-ene-1-carboxylate:CoA ligase); Cyclohexanecarboxylate-CoA ligase activates the aliphatic ring compound, cyclohexanecarboxylate, for degradation. It catalyzes the synthesis of cyclohexanecarboxylate-CoA thioesters in a two-step reaction involving the formation of cyclohexanecarboxylate-AMP anhydride, followed by the nucleophilic substitution of AMP by CoA.
Pssm-ID: 341229 [Multi-domain] Cd Length: 437 Bit Score: 247.68 E-value: 5.74e-76
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 77 SYKELDDHSRALAAWLQSRGLVKGDRVAIMMPNILQYPITFTAVLRAGFVVVNVNPLYTPRELEHQLNDAGAKALVVLEN 156
Cdd:cd05903 3 TYSELDTRADRLAAGLAALGVGPGDVVAFQLPNWWEFAVLYLACLRIGAVTNPILPFFREHELAFILRRAKAKVFVVPER 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 157 FattvekalpsinvpnilvasmgdmlgfkghivnlvvrrvkkmvpawnipghvrfkdalaqgraKSFNPVPvQGCDIAFL 236
Cdd:cd05903 83 F---------------------------------------------------------------RQFDPAA-MPDAVALL 98
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 237 QYTGGTTGISKGAMLTHSNILANVEQMnlwmdvaFRNKGKPKALNFVCALPLYHIFALTVNAMIGMKLGARNIL--IPNP 314
Cdd:cd05903 99 LFTSGTTGEPKGVMHSHNTLSASIRQY-------AERLGLGPGDVFLVASPMAHQTGFVYGFTLPLLLGAPVVLqdIWDP 171
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 315 RDIPSFVKELKkypVHIFPGLNTLFNGLMNNPDFQTLDFKPLILTLGGGMAVQRPVAERWQHMTGCHITEGYGLSET-SP 393
Cdd:cd05903 172 DKALALMREHG---VTFMMGATPFLTDLLNAVEEAGEPLSRLRTFVCGGATVPRSLARRAAELLGAKVCSAYGSTECpGA 248
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 394 VACANALDATEFTGTIGLPMPSTDVVIRDDDGSDLLLGKVGEICVRGPQVMKGYWNRPEETARAiMADGFFRTGDMGFMD 473
Cdd:cd05903 249 VTSITPAPEDRRLYTDGRPLPGVEIKVVDDTGATLAPGVEGELLSRGPSVFLGYLDRPDLTADA-APEGWFRTGDLARLD 327
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 474 ERGYTKIVDRKKDMILVSGFNVYPNEIEEVAAEHPGVLESAAVGIPNEHSGEVVKLYVVRRDP-ELTEDEVKAFC-AERL 551
Cdd:cd05903 328 EDGYLRITGRSKDIIIRGGENIPVLEVEDLLLGHPGVIEAAVVALPDERLGERACAVVVTKSGaLLTFDELVAYLdRQGV 407
|
490 500
....*....|....*....|....*....
gi 479816261 552 TNYKRPREVEFRESLPKSNVGKILRRELR 580
Cdd:cd05903 408 AKQYWPERLVHVDDLPRTPSGKVQKFRLR 436
|
|
| BCL_like |
cd05919 |
Benzoate CoA ligase (BCL) and similar adenylate forming enzymes; This family contains benzoate ... |
67-580 |
1.11e-75 |
|
Benzoate CoA ligase (BCL) and similar adenylate forming enzymes; This family contains benzoate CoA ligase (BCL) and related ligases that catalyze the acylation of benzoate derivatives, 2-aminobenzoate and 4-hydroxybenzoate. Aromatic compounds represent the second most abundant class of organic carbon compounds after carbohydrates. Xenobiotic aromatic compounds are also a major class of man-made pollutants. Some bacteria use benzoate as the sole source of carbon and energy through benzoate degradation. Benzoate degradation starts with its activation to benzoyl-CoA by benzoate CoA ligase. The reaction catalyzed by benzoate CoA ligase proceeds via a two-step process; the first ATP-dependent step forms an acyl-AMP intermediate, and the second step forms the acyl-CoA ester with release of the AMP.
Pssm-ID: 341243 [Multi-domain] Cd Length: 436 Bit Score: 246.99 E-value: 1.11e-75
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 67 PAFTCMGKDISYKELDDHSRALAAWLQSRGLVKGDRVAIMMPNILQYPITFTAVLRAGFVVVNVNPLYTPRELEHQLNDA 146
Cdd:cd05919 2 TAFYAADRSVTYGQLHDGANRLGSALRNLGVSSGDRVLLLMLDSPELVQLFLGCLARGAIAVVINPLLHPDDYAYIARDC 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 147 GAKALVVlenfattvekalpsinvpnilvasmgdmlgfkghivnlvvrrvkkmvpawnipghvrfkdalaqgraksfnpv 226
Cdd:cd05919 82 EARLVVT------------------------------------------------------------------------- 88
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 227 pvQGCDIAFLQYTGGTTGISKGAMLTHSNILANVEqmnLWMDVAFRNKGKPKALnfvCALPLYHIFALTVNAMIGMKLGA 306
Cdd:cd05919 89 --SADDIAYLLYSSGTTGPPKGVMHAHRDPLLFAD---AMAREALGLTPGDRVF---SSAKMFFGYGLGNSLWFPLAVGA 160
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 307 RNILIPNPRDIPSFVKELKKYPVHIFPGLNTLFNGLMNNPDFQTLDFKPLILTLGGGMAVQRPVAERWQHMTGCHITEGY 386
Cdd:cd05919 161 SAVLNPGWPTAERVLATLARFRPTVLYGVPTFYANLLDSCAGSPDALRSLRLCVSAGEALPRGLGERWMEHFGGPILDGI 240
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 387 GLSETSPVACANALDATEFtGTIGLPMPSTDVVIRDDDGSDLLLGKVGEICVRGPQVMKGYWNRPEETaRAIMADGFFRT 466
Cdd:cd05919 241 GATEVGHIFLSNRPGAWRL-GSTGRPVPGYEIRLVDEEGHTIPPGEEGDLLVRGPSAAVGYWNNPEKS-RATFNGGWYRT 318
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 467 GDMGFMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVAAEHPGVLESAAVGIPNEHSGEVVKLYVVRRDP----ELTEDE 542
Cdd:cd05919 319 GDKFCRDADGWYTHAGRADDMLKVGGQWVSPVEVESLIIQHPAVAEAAVVAVPESTGLSRLTAFVVLKSPaapqESLARD 398
|
490 500 510
....*....|....*....|....*....|....*...
gi 479816261 543 VKAFCAERLTNYKRPREVEFRESLPKSNVGKILRRELR 580
Cdd:cd05919 399 IHRHLLERLSAHKVPRRIAFVDELPRTATGKLQRFKLR 436
|
|
| ttLC_FACS_AlkK_like |
cd12119 |
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles; This family includes ... |
77-581 |
3.28e-75 |
|
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles; This family includes fatty acyl-CoA synthetases that can activate medium-chain to long-chain fatty acids. They catalyze the ATP-dependent acylation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. The fatty acyl-CoA synthetases are responsible for fatty acid degradation as well as physiological regulation of cellular functions via the production of fatty acyl-CoA esters. The fatty acyl-CoA synthetase from Thermus thermophiles in this family catalyzes the long-chain fatty acid, myristoyl acid, while another member in this family, the AlkK protein identified from Pseudomonas oleovorans, targets medium chain fatty acids. This family also includes uncharacterized FACS proteins.
Pssm-ID: 341284 [Multi-domain] Cd Length: 518 Bit Score: 248.31 E-value: 3.28e-75
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 77 SYKELDDHSRALAAWLQSRGLVKGDRVAIMMPNILQYPITFTAVLRAGFVVVNVNPLYTPRELEHQLNDAGAKALVVLEN 156
Cdd:cd12119 27 TYAEVAERARRLANALRRLGVKPGDRVATLAWNTHRHLELYYAVPGMGAVLHTINPRLFPEQIAYIINHAEDRVVFVDRD 106
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 157 FATTVEKALPSI-NVPNILVASMGDMLGFKGhivnlvvrrvkkmvpawnIPGHVRFKDALAQGRAKSFNPVpVQGCDIAF 235
Cdd:cd12119 107 FLPLLEAIAPRLpTVEHVVVMTDDAAMPEPA------------------GVGVLAYEELLAAESPEYDWPD-FDENTAAA 167
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 236 LQYTGGTTGISKGAMLTH-SNIL-----ANVEQMNLW-MDVafrnkgkpkalnFVCALPLYHifaltVNA--------MI 300
Cdd:cd12119 168 ICYTSGTTGNPKGVVYSHrSLVLhamaaLLTDGLGLSeSDV------------VLPVVPMFH-----VNAwglpyaaaMV 230
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 301 GMKLgarniLIPNPRDIPSFVKEL-KKYPVHIFPGLNTLFNGLMNNPDFQTLDFKPLILTLGGGMAVQRPVAERWQHMtG 379
Cdd:cd12119 231 GAKL-----VLPGPYLDPASLAELiEREGVTFAAGVPTVWQGLLDHLEANGRDLSSLRRVVIGGSAVPRSLIEAFEER-G 304
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 380 CHITEGYGLSETSPVACANALDA----------TEFTGTIGLPMPSTDVVIRDDDGSDLLL-GK-VGEICVRGPQVMKGY 447
Cdd:cd12119 305 VRVIHAWGMTETSPLGTVARPPSehsnlsedeqLALRAKQGRPVPGVELRIVDDDGRELPWdGKaVGELQVRGPWVTKSY 384
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 448 WNRPEETArAIMADGFFRTGDMGFMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVAAEHPGVLESAAVGIPNEHSGEVV 527
Cdd:cd12119 385 YKNDEESE-ALTEDGWLRTGDVATIDEDGYLTITDRSKDVIKSGGEWISSVELENAIMAHPAVAEAAVIGVPHPKWGERP 463
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*
gi 479816261 528 KLYVVRRDPE-LTEDEVKAFCAERLTNYKRPREVEFRESLPKSNVGKILRRELRD 581
Cdd:cd12119 464 LAVVVLKEGAtVTAEELLEFLADKVAKWWLPDDVVFVDEIPKTSTGKIDKKALRE 518
|
|
| FadD3 |
cd17638 |
acyl-CoA synthetase FadD3 and similar proteins; This family contains long chain fatty acid CoA ... |
232-576 |
8.28e-75 |
|
acyl-CoA synthetase FadD3 and similar proteins; This family contains long chain fatty acid CoA ligases, including FadD3 which is an acyl-CoA synthetase that initiates catabolism of cholesterol rings C and D in actinobacteria. The cholesterol catabolic pathway occurs in most mycolic acid-containing actinobacteria, such as Rhodococcus jostii RHA1, and is critical for Mycobacterium tuberculosis (Mtb) during infection. FadD3 catalyzes the ATP-dependent CoA thioesterification of 3a-alpha-H-4alpha(3'-propanoate)-7a-beta-methylhexahydro-1,5-indanedione (HIP) to yield HIP-CoA. Hydroxylated analogs of HIP, 5alpha-OH HIP and 1beta-OH HIP, can also be used.
Pssm-ID: 341293 [Multi-domain] Cd Length: 330 Bit Score: 241.25 E-value: 8.28e-75
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 232 DIAFLQYTGGTTGISKGAMLTHsniLANVEQMNLWMDVAFRNKGKpkalNFVCALPLYHIFALTVNAMIGMKLGArnILI 311
Cdd:cd17638 1 DVSDIMFTSGTTGRSKGVMCAH---RQTLRAAAAWADCADLTEDD----RYLIINPFFHTFGYKAGIVACLLTGA--TVV 71
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 312 PNPR-DIPSFVKELKKYPVHIFPGLNTLFNGLMNNPDFQTLDFKPLILTLGGGMAVQRPVAERWQHMTGCH-ITEGYGLS 389
Cdd:cd17638 72 PVAVfDVDAILEAIERERITVLPGPPTLFQSLLDHPGRKKFDLSSLRAAVTGAATVPVELVRRMRSELGFEtVLTAYGLT 151
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 390 ETSPVA-CANALDATEFTGTIGLPMPSTDVVIRDDdgsdlllgkvGEICVRGPQVMKGYWNRPEETARAIMADGFFRTGD 468
Cdd:cd17638 152 EAGVATmCRPGDDAETVATTCGRACPGFEVRIADD----------GEVLVRGYNVMQGYLDDPEATAEAIDADGWLHTGD 221
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 469 MGFMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVAAEHPGVLESAAVGIPNEHSGEVVKLYVVRRDPE-LTEDEVKAFC 547
Cdd:cd17638 222 VGELDERGYLRITDRLKDMYIVGGFNVYPAEVEGALAEHPGVAQVAVIGVPDERMGEVGKAFVVARPGVtLTEEDVIAWC 301
|
330 340
....*....|....*....|....*....
gi 479816261 548 AERLTNYKRPREVEFRESLPKSNVGKILR 576
Cdd:cd17638 302 RERLANYKVPRFVRFLDELPRNASGKVMK 330
|
|
| VL_LC_FACS_like |
cd05907 |
Long-chain fatty acid CoA synthetases and Bubblegum-like very long-chain fatty acid CoA ... |
74-517 |
6.11e-73 |
|
Long-chain fatty acid CoA synthetases and Bubblegum-like very long-chain fatty acid CoA synthetases; This family includes long-chain fatty acid (C12-C20) CoA synthetases and Bubblegum-like very long-chain (>C20) fatty acid CoA synthetases. FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Eukaryotes generally have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells. Drosophila melanogaster mutant bubblegum (BGM) have elevated levels of very-long-chain fatty acids (VLCFA) caused by a defective gene later named bubblegum. The human homolog (hsBG) of bubblegum has been characterized as a very long chain fatty acid CoA synthetase that functions specifically in the brain; hsBG may play a central role in brain VLCFA metabolism and myelinogenesis. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.
Pssm-ID: 341233 [Multi-domain] Cd Length: 452 Bit Score: 240.19 E-value: 6.11e-73
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 74 KDISYKELDDHSRALAAWLQSRGLVKGDRVAIMMPNILQYPITFTAVLRAGFVVVnvnPLY---TPRELEHQLNDAGAKA 150
Cdd:cd05907 4 QPITWAEFAEEVRALAKGLIALGVEPGDRVAILSRNRPEWTIADLAILAIGAVPV---PIYptsSAEQIAYILNDSEAKA 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 151 LVVlENfattvekalPSinvpnilvasmgdmlgfkghivnlvvrrvkkmvpawnipghvrfkdalaqgraksfnpvpvqg 230
Cdd:cd05907 81 LFV-ED---------PD--------------------------------------------------------------- 87
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 231 cDIAFLQYTGGTTGISKGAMLTHSNILANV----EQMNLWMDVAFrnkgkpkaLNFvcaLPLYHIFALTVNAMIGMKLGA 306
Cdd:cd05907 88 -DLATIIYTSGTTGRPKGVMLSHRNILSNAlalaERLPATEGDRH--------LSF---LPLAHVFERRAGLYVPLLAGA 155
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 307 RNILIPNPRDIPSFVKELKkyPvHIFPG----LNTLFNGLMNN--PDFQTLDFKPLIL-----TLGGGMAVQRPVAERWQ 375
Cdd:cd05907 156 RIYFASSAETLLDDLSEVR--P-TVFLAvprvWEKVYAAIKVKavPGLKRKLFDLAVGgrlrfAASGGAPLPAELLHFFR 232
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 376 HMtGCHITEGYGLSETSPVACANALDATEFtGTIGLPMPSTDVVIRDDdgsdlllgkvGEICVRGPQVMKGYWNRPEETA 455
Cdd:cd05907 233 AL-GIPVYEGYGLTETSAVVTLNPPGDNRI-GTVGKPLPGVEVRIADD----------GEILVRGPNVMLGYYKNPEATA 300
|
410 420 430 440 450 460
....*....|....*....|....*....|....*....|....*....|....*....|...
gi 479816261 456 RAIMADGFFRTGDMGFMDERGYTKIVDRKKDMILVS-GFNVYPNEIEEVAAEHPGVLESAAVG 517
Cdd:cd05907 301 EALDADGWLHTGDLGEIDEDGFLHITGRKKDLIITSgGKNISPEPIENALKASPLISQAVVIG 363
|
|
| PRK03640 |
PRK03640 |
o-succinylbenzoate--CoA ligase; |
65-581 |
2.62e-72 |
|
o-succinylbenzoate--CoA ligase;
Pssm-ID: 235146 [Multi-domain] Cd Length: 483 Bit Score: 239.48 E-value: 2.62e-72
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 65 NSPAFTCMGKDISYKELDDHSRALAAWLQSRGLVKGDRVAIMMPNILQYPITFTAVLRAGFVVVNVNPLYTPRELEHQLN 144
Cdd:PRK03640 17 DRTAIEFEEKKVTFMELHEAVVSVAGKLAALGVKKGDRVALLMKNGMEMILVIHALQQLGAVAVLLNTRLSREELLWQLD 96
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 145 DAGAKALVVLENFATTVEkalpsinvpnilvasmgdmlgfkGHIVNLVvrrvkkmvpawnipghvrfkDALAQGRAKSFN 224
Cdd:PRK03640 97 DAEVKCLITDDDFEAKLI-----------------------PGISVKF--------------------AELMNGPKEEAE 133
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 225 PV-PVQGCDIAFLQYTGGTTGISKGAMLTHSNilanveqmNLWMDVAfrnkgkpKALN--------FVCALPLYHIFALT 295
Cdd:PRK03640 134 IQeEFDLDEVATIMYTSGTTGKPKGVIQTYGN--------HWWSAVG-------SALNlglteddcWLAAVPIFHISGLS 198
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 296 VnAMIGMKLGARNILIP--NPRDI---------------PSFVKEL-KKYPVHIFPglntlfnglmnnPDFQTLdfkpli 357
Cdd:PRK03640 199 I-LMRSVIYGMRVVLVEkfDAEKInkllqtggvtiisvvSTMLQRLlERLGEGTYP------------SSFRCM------ 259
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 358 lTLGGGMAvQRPVAERWQHmTGCHITEGYGLSET-SPVACANALDATEFTGTIGLPMPSTDVVIRDDdGSDLLLGKVGEI 436
Cdd:PRK03640 260 -LLGGGPA-PKPLLEQCKE-KGIPVYQSYGMTETaSQIVTLSPEDALTKLGSAGKPLFPCELKIEKD-GVVVPPFEEGEI 335
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 437 CVRGPQVMKGYWNRPEETARAiMADGFFRTGDMGFMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVAAEHPGVLESAAV 516
Cdd:PRK03640 336 VVKGPNVTKGYLNREDATRET-FQDGWFKTGDIGYLDEEGFLYVLDRRSDLIISGGENIYPAEIEEVLLSHPGVAEAGVV 414
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 479816261 517 GIPNEHSGEVVKLYVVrRDPELTEDEVKAFCAERLTNYKRPREVEFRESLPKSNVGKILRRELRD 581
Cdd:PRK03640 415 GVPDDKWGQVPVAFVV-KSGEVTEEELRHFCEEKLAKYKVPKRFYFVEELPRNASGKLLRHELKQ 478
|
|
| PRK12406 |
PRK12406 |
long-chain-fatty-acid--CoA ligase; Provisional |
77-581 |
1.82e-71 |
|
long-chain-fatty-acid--CoA ligase; Provisional
Pssm-ID: 183506 [Multi-domain] Cd Length: 509 Bit Score: 238.06 E-value: 1.82e-71
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 77 SYKELDDHSRALAAWLQSRGLVKGDRVAIMMPNILQYPITFTAVLRAGFVVVNVNPLYTPRELEHQLNDAGAKALVVLEN 156
Cdd:PRK12406 13 SFDELAQRAARAAGGLAALGVRPGDCVALLMRNDFAFFEAAYAAMRLGAYAVPVNWHFKPEEIAYILEDSGARVLIAHAD 92
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 157 FATTVEKALPSiNVPNILVASMGDMLGfkghivNLVVRRVKKMVPawniPGHVRFKDALAQGRAKSFNPVPVQGCDIafl 236
Cdd:PRK12406 93 LLHGLASALPA-GVTVLSVPTPPEIAA------AYRISPALLTPP----AGAIDWEGWLAQQEPYDGPPVPQPQSMI--- 158
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 237 qYTGGTTGISKGAMLTHsnilANVEQMNLWMDVAFRNKGKPKALNFVCALPLYHIfALTVNAMIGMKLGARNILIP--NP 314
Cdd:PRK12406 159 -YTSGTTGHPKGVRRAA----PTPEQAAAAEQMRALIYGLKPGIRALLTGPLYHS-APNAYGLRAGRLGGVLVLQPrfDP 232
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 315 RDIPSFVKELKKYPVHIFPglnTLFNGLMNNP-------DFQTLDFkpliLTLGGGMA---VQRPVAERWqhmtGCHITE 384
Cdd:PRK12406 233 EELLQLIERHRITHMHMVP---TMFIRLLKLPeevrakyDVSSLRH----VIHAAAPCpadVKRAMIEWW----GPVIYE 301
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 385 GYGLSETSPVACANALDATEFTGTIGLPMPSTDVVIRDDDGSDLLLGKVGEICVRGPQVMK-GYWNRPEETArAIMADGF 463
Cdd:PRK12406 302 YYGSTESGAVTFATSEDALSHPGTVGKAAPGAELRFVDEDGRPLPQGEIGEIYSRIAGNPDfTYHNKPEKRA-EIDRGGF 380
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 464 FRTGDMGFMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVAAEHPGVLESAAVGIPNEHSGEVVKLyVVRRDP--ELTED 541
Cdd:PRK12406 381 ITSGDVGYLDADGYLFLCDRKRDMVISGGVNIYPAEIEAVLHAVPGVHDCAVFGIPDAEFGEALMA-VVEPQPgaTLDEA 459
|
490 500 510 520
....*....|....*....|....*....|....*....|
gi 479816261 542 EVKAFCAERLTNYKRPREVEFRESLPKSNVGKILRRELRD 581
Cdd:PRK12406 460 DIRAQLKARLAGYKVPKHIEIMAELPREDSGKIFKRRLRD 499
|
|
| PRK08276 |
PRK08276 |
long-chain-fatty-acid--CoA ligase; Validated |
73-581 |
1.96e-71 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 236215 [Multi-domain] Cd Length: 502 Bit Score: 237.88 E-value: 1.96e-71
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 73 GKDISYKELDDHSRALAAWLQSRGLVKGDRVAIMMPNILQYPITFTAVLRAGFVVVNVNPLYTPRELEHQLNDAGAKALV 152
Cdd:PRK08276 9 GEVVTYGELEARSNRLAHGLRALGLREGDVVAILLENNPEFFEVYWAARRSGLYYTPINWHLTAAEIAYIVDDSGAKVLI 88
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 153 VLENFATTVEKALPSINVPnilvasmgdmlgfkghivnLVVRrvkkMVPAWNIPGHVRFKDALAqgrAKSFNPVPVQ--G 230
Cdd:PRK08276 89 VSAALADTAAELAAELPAG-------------------VPLL----LVVAGPVPGFRSYEEALA---AQPDTPIADEtaG 142
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 231 CDiafLQYTGGTTGISKGAM--LTHSNILANVEQMnLWMdVAFRNKGKPKALNFVCAlPLYHIfALTVNAMIGMKLGARN 308
Cdd:PRK08276 143 AD---MLYSSGTTGRPKGIKrpLPGLDPDEAPGMM-LAL-LGFGMYGGPDSVYLSPA-PLYHT-APLRFGMSALALGGTV 215
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 309 ILIPNpRDIPSFVKELKKYPV---HIFPglnTLFNGLMNNPDF--QTLDFKPLILTLGGG----MAVQRPVAERWqhmtG 379
Cdd:PRK08276 216 VVMEK-FDAEEALALIERYRVthsQLVP---TMFVRMLKLPEEvrARYDVSSLRVAIHAAapcpVEVKRAMIDWW----G 287
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 380 CHITEGYGLSETSPVACANALDATEFTGTIGLPMPSTdVVIRDDDGSDLLLGKVGEICVRGPQVMKGYWNRPEETARAIM 459
Cdd:PRK08276 288 PIIHEYYASSEGGGVTVITSEDWLAHPGSVGKAVLGE-VRILDEDGNELPPGEIGTVYFEMDGYPFEYHNDPEKTAAARN 366
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 460 ADGFFRTGDMGFMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVAAEHPGVLESAAVGIPNEHSGEVVKLYV-----VRR 534
Cdd:PRK08276 367 PHGWVTVGDVGYLDEDGYLYLTDRKSDMIISGGVNIYPQEIENLLVTHPKVADVAVFGVPDEEMGERVKAVVqpadgADA 446
|
490 500 510 520
....*....|....*....|....*....|....*....|....*..
gi 479816261 535 DPELTEdEVKAFCAERLTNYKRPREVEFRESLPKSNVGKILRRELRD 581
Cdd:PRK08276 447 GDALAA-ELIAWLRGRLAHYKCPRSIDFEDELPRTPTGKLYKRRLRD 492
|
|
| PRK07470 |
PRK07470 |
acyl-CoA synthetase; Validated |
49-581 |
1.05e-70 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 180988 [Multi-domain] Cd Length: 528 Bit Score: 236.48 E-value: 1.05e-70
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 49 ATSISDMISASCRQFANSPAFTCMGKDISYKELDDHSRALAAWLQSRGLVKGDRVAIMMPNILQYPITFTAVLRAGFVVV 128
Cdd:PRK07470 6 VMNLAHFLRQAARRFPDRIALVWGDRSWTWREIDARVDALAAALAARGVRKGDRILVHSRNCNQMFESMFAAFRLGAVWV 85
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 129 NVNPLYTPRELEHQLNDAGAKALVVLENFATTVEkalpsinvpniLVASMGDMLGFKghivnlvvrrvkkmVPAWNIPGH 208
Cdd:PRK07470 86 PTNFRQTPDEVAYLAEASGARAMICHADFPEHAA-----------AVRAASPDLTHV--------------VAIGGARAG 140
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 209 VRFKDALAQGRAKSFNPVPVQGCDIAFLQYTGGTTGISKGAMLTH-------SNILANVEQMNLWMDVAFrnkgkpkaln 281
Cdd:PRK07470 141 LDYEALVARHLGARVANAAVDHDDPCWFFFTSGTTGRPKAAVLTHgqmafviTNHLADLMPGTTEQDASL---------- 210
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 282 fVCAlPLYH---IFALTVNAMigmklGARNILIPNPR-DIPSFVKELKKYPVHIFPGLNTLFNGLMNNPDFQTLDFKPLI 357
Cdd:PRK07470 211 -VVA-PLSHgagIHQLCQVAR-----GAATVLLPSERfDPAEVWALVERHRVTNLFTVPTILKMLVEHPAVDRYDHSSLR 283
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 358 LTLGGGMAVQRPVAERWQHMTGCHITEGYGLSETS------PVACANALDATEF-TGTIGLPMPSTDVVIRDDDGSDLLL 430
Cdd:PRK07470 284 YVIYAGAPMYRADQKRALAKLGKVLVQYFGLGEVTgnitvlPPALHDAEDGPDArIGTCGFERTGMEVQIQDDEGRELPP 363
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 431 GKVGEICVRGPQVMKGYWNRPEETARAiMADGFFRTGDMGFMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVAAEHPGV 510
Cdd:PRK07470 364 GETGEICVIGPAVFAGYYNNPEANAKA-FRDGWFRTGDLGHLDARGFLYITGRASDMYISGGSNVYPREIEEKLLTHPAV 442
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 479816261 511 LESAAVGIPNEHSGEVVKLYVVRRDP-ELTEDEVKAFCAERLTNYKRPREVEFRESLPKSNVGKILRRELRD 581
Cdd:PRK07470 443 SEVAVLGVPDPVWGEVGVAVCVARDGaPVDEAELLAWLDGKVARYKLPKRFFFWDALPKSGYGKITKKMVRE 514
|
|
| PLN02574 |
PLN02574 |
4-coumarate--CoA ligase-like |
37-581 |
5.84e-69 |
|
4-coumarate--CoA ligase-like
Pssm-ID: 215312 [Multi-domain] Cd Length: 560 Bit Score: 232.81 E-value: 5.84e-69
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 37 PAGVPHDINLSAatsISDMISASCRQFANSPAFTCMGKDISYKELDDHSRALAAWLQSR-GLVKGDRVAIMMPNILQYPI 115
Cdd:PLN02574 31 PVPLPSDPNLDA---VSFIFSHHNHNGDTALIDSSTGFSISYSELQPLVKSMAAGLYHVmGVRQGDVVLLLLPNSVYFPV 107
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 116 TFTAVLRAGFVVVNVNPLYTPRELEHQLNDAGAK-ALVVLENfattVEKaLPSINVPNILVASMGDMLGfkghivnlvvR 194
Cdd:PLN02574 108 IFLAVLSLGGIVTTMNPSSSLGEIKKRVVDCSVGlAFTSPEN----VEK-LSPLGVPVIGVPENYDFDS----------K 172
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 195 RVKKMVPAWNIPGhvrfkdalaqgrakSFNPVP---VQGCDIAFLQYTGGTTGISKGAMLTHSNILANVEQMNLWMDVAF 271
Cdd:PLN02574 173 RIEFPKFYELIKE--------------DFDFVPkpvIKQDDVAAIMYSSGTTGASKGVVLTHRNLIAMVELFVRFEASQY 238
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 272 RNKGKPKAlnFVCALPLYHIFALTVNAMIGMKLGArNILIPNPRDIPSFVKELKKYPVHIFPGLNTLFNGLMNNPD-FQT 350
Cdd:PLN02574 239 EYPGSDNV--YLAALPMFHIYGLSLFVVGLLSLGS-TIVVMRRFDASDMVKVIDRFKVTHFPVVPPILMALTKKAKgVCG 315
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 351 LDFKPLIL-TLGGGMAVQRPVAERWQHMTGCHITEGYGLSETSPVAcANALDATEFT--GTIGLPMPSTDVVIRD-DDGS 426
Cdd:PLN02574 316 EVLKSLKQvSCGAAPLSGKFIQDFVQTLPHVDFIQGYGMTESTAVG-TRGFNTEKLSkySSVGLLAPNMQAKVVDwSTGC 394
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 427 DLLLGKVGEICVRGPQVMKGYWNRPEETARAIMADGFFRTGDMGFMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVAAE 506
Cdd:PLN02574 395 LLPPGNCGELWIQGPGVMKGYLNNPKATQSTIDKDGWLRTGDIAYFDEDGYLYIVDRLKEIIKYKGFQIAPADLEAVLIS 474
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 479816261 507 HPGVLESAAVGIPNEHSGEVVKLYVVRR-DPELTEDEVKAFCAERLTNYKRPREVEFRESLPKSNVGKILRRELRD 581
Cdd:PLN02574 475 HPEIIDAAVTAVPDKECGEIPVAFVVRRqGSTLSQEAVINYVAKQVAPYKKVRKVVFVQSIPKSPAGKILRRELKR 550
|
|
| PLN02330 |
PLN02330 |
4-coumarate--CoA ligase-like 1 |
29-581 |
8.93e-69 |
|
4-coumarate--CoA ligase-like 1
Pssm-ID: 215189 [Multi-domain] Cd Length: 546 Bit Score: 232.18 E-value: 8.93e-69
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 29 EKIWLKSYPA-GVPHDInlsaatSISDMISASCRQFANSPAFT--CMGKDISYKELDDHSRALAAWLQSRGLVKGDRVAI 105
Cdd:PLN02330 12 EHIFRSRYPSvPVPDKL------TLPDFVLQDAELYADKVAFVeaVTGKAVTYGEVVRDTRRFAKALRSLGLRKGQVVVV 85
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 106 MMPNILQYPITFTAVLRAGFVVVNVNPLYTPRELEHQLNDAGAKALVVlenFATTVEKaLPSINVPNILVASMgdmlgfk 185
Cdd:PLN02330 86 VLPNVAEYGIVALGIMAAGGVFSGANPTALESEIKKQAEAAGAKLIVT---NDTNYGK-VKGLGLPVIVLGEE------- 154
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 186 ghivnlvvrrvkkmvpawNIPGHVRFKDAL-AQGRA--KSFNPVPVQgCDIAFLQYTGGTTGISKGAMLTHSNILANVeq 262
Cdd:PLN02330 155 ------------------KIEGAVNWKELLeAADRAgdTSDNEEILQ-TDLCALPFSSGTTGISKGVMLTHRNLVANL-- 213
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 263 MNLWMDVAFRNKGKPKALNFVcalPLYHIFALTVNAMIGMKLGARnILIPNPRDIPSFVKELKKYPVHIFPGLNTLFNGL 342
Cdd:PLN02330 214 CSSLFSVGPEMIGQVVTLGLI---PFFHIYGITGICCATLRNKGK-VVVMSRFELRTFLNALITQEVSFAPIVPPIILNL 289
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 343 MNNPDFQTLDFKPLilTLGGGMAVQRPVAERW-----QHMTGCHITEGYGLSETSPVACANAlDATEFTG-----TIGLP 412
Cdd:PLN02330 290 VKNPIVEEFDLSKL--KLQAIMTAAAPLAPELltafeAKFPGVQVQEAYGLTEHSCITLTHG-DPEKGHGiakknSVGFI 366
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 413 MPSTDV-VIRDDDGSDLLLGKVGEICVRGPQVMKGYWNRPEETARAIMADGFFRTGDMGFMDERGYTKIVDRKKDMILVS 491
Cdd:PLN02330 367 LPNLEVkFIDPDTGRSLPKNTPGELCVRSQCVMQGYYNNKEETDRTIDEDGWLHTGDIGYIDDDGDIFIVDRIKELIKYK 446
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 492 GFNVYPNEIEEVAAEHPGVLESAAVGIPNEHSGEV-VKLYVVRRDPELTEDEVKAFCAERLTNYKRPREVEFRESLPKSN 570
Cdd:PLN02330 447 GFQVAPAELEAILLTHPSVEDAAVVPLPDEEAGEIpAACVVINPKAKESEEDILNFVAANVAHYKKVRVVQFVDSIPKSL 526
|
570
....*....|.
gi 479816261 571 VGKILRRELRD 581
Cdd:PLN02330 527 SGKIMRRLLKE 537
|
|
| PRK06145 |
PRK06145 |
acyl-CoA synthetase; Validated |
51-581 |
3.38e-68 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 102207 [Multi-domain] Cd Length: 497 Bit Score: 229.00 E-value: 3.38e-68
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 51 SISDMISASCRQFANSPAFTCMGKDISYKELDDHSRALAAWLQSRGLVKGDRVAIMMPNILQYPITFTAVLRAGFVVVNV 130
Cdd:PRK06145 3 NLSASIAFHARRTPDRAALVYRDQEISYAEFHQRILQAAGMLHARGIGQGDVVALLMKNSAAFLELAFAASYLGAVFLPI 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 131 NPLYTPRELEHQLNDAGAKALVVLENFAttvekALPSINVPNILVasmgdmlgfkGHIVNLVVRRvkkmvpawnipghvr 210
Cdd:PRK06145 83 NYRLAADEVAYILGDAGAKLLLVDEEFD-----AIVALETPKIVI----------DAAAQADSRR--------------- 132
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 211 fkdaLAQGRAKSFNPVPVQGCDIAFLQYTGGTTGISKGAMLTHSNILanveqmnlWmdvafrnkgkpKALNFVCAL---- 286
Cdd:PRK06145 133 ----LAQGGLEIPPQAAVAPTDLVRLMYTSGTTDRPKGVMHSYGNLH--------W-----------KSIDHVIALglta 189
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 287 --------PLYHIFA-----LTVNAMIGMKLGARNIlipNPRDIPSFVKELKKYPVHIFPglnTLFNGLMNNPDFQTLDF 353
Cdd:PRK06145 190 serllvvgPLYHVGAfdlpgIAVLWVGGTLRIHREF---DPEAVLAAIERHRLTCAWMAP---VMLSRVLTVPDRDRFDL 263
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 354 KPLILTLGGGMAV-QRPVAERWQHMTGCHITEGYGLSET-SPVACANALDATEFTGTIGLPMPSTDVVIRDDDGSDLLLG 431
Cdd:PRK06145 264 DSLAWCIGGGEKTpESRIRDFTRVFTRARYIDAYGLTETcSGDTLMEAGREIEKIGSTGRALAHVEIRIADGAGRWLPPN 343
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 432 KVGEICVRGPQVMKGYWNRPEETARAIMaDGFFRTGDMGFMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVAAEHPGVL 511
Cdd:PRK06145 344 MKGEICMRGPKVTKGYWKDPEKTAEAFY-GDWFRSGDVGYLDEEGFLYLTDRKKDMIISGGENIASSEVERVIYELPEVA 422
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 479816261 512 ESAAVGIPNEHSGE-VVKLYVVRRDPELTEDEVKAFCAERLTNYKRPREVEFRESLPKSNVGKILRRELRD 581
Cdd:PRK06145 423 EAAVIGVHDDRWGErITAVVVLNPGATLTLEALDRHCRQRLASFKVPRQLKVRDELPRNPSGKVLKRVLRD 493
|
|
| ttLC_FACS_AEE21_like |
cd12118 |
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles and Arabidopsis; This ... |
77-581 |
5.08e-66 |
|
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles and Arabidopsis; This family includes fatty acyl-CoA synthetases that can activate medium to long-chain fatty acids. These enzymes catalyze the ATP-dependent acylation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. Fatty acyl-CoA synthetases are responsible for fatty acid degradation as well as physiological regulation of cellular functions via the production of fatty acyl-CoA esters. The fatty acyl-CoA synthetase from Thermus thermophiles in this family has been shown to catalyze the long-chain fatty acid, myristoyl acid. Also included in this family are acyl activating enzymes from Arabidopsis, which contains a large number of proteins from this family with up to 63 different genes, many of which are uncharacterized.
Pssm-ID: 341283 [Multi-domain] Cd Length: 486 Bit Score: 222.94 E-value: 5.08e-66
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 77 SYKELDDHSRALAAWLQSRGLVKGDRVAIMMPNILQYPITFTAVLRAGFVVVNVNPLYTPRELEHQLNDAGAKALVVLEN 156
Cdd:cd12118 31 TWRQTYDRCRRLASALAALGISRGDTVAVLAPNTPAMYELHFGVPMAGAVLNALNTRLDAEEIAFILRHSEAKVLFVDRE 110
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 157 FAttvekalpsinvpnilvasmgdmlgfkghivnlvvrrvkkmvpawnipghvrFKDALAQGRaKSFNPVPVQG-CDIAF 235
Cdd:cd12118 111 FE----------------------------------------------------YEDLLAEGD-PDFEWIPPADeWDPIA 137
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 236 LQYTGGTTGISKGAMLTHS----NILANVeqmnlwmdVAFRNKGKPKALnfvCALPLYHI----FALTVNAmigmkLGAR 307
Cdd:cd12118 138 LNYTSGTTGRPKGVVYHHRgaylNALANI--------LEWEMKQHPVYL---WTLPMFHCngwcFPWTVAA-----VGGT 201
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 308 NILIPNPrDIPSFVKELKKYPVHIFPGLNTLFNGLMNNPDFQTLDFKPLILTLGGGMAVQRPVAERWQHMtGCHITEGYG 387
Cdd:cd12118 202 NVCLRKV-DAKAIYDLIEKHKVTHFCGAPTVLNMLANAPPSDARPLPHRVHVMTAGAPPPAAVLAKMEEL-GFDVTHVYG 279
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 388 LSETSPVA--CA-----NALDATEFTGTI---GLPMPSTD-VVIRDDDGSDLLL--GK-VGEICVRGPQVMKGYWNRPEE 453
Cdd:cd12118 280 LTETYGPAtvCAwkpewDELPTEERARLKarqGVRYVGLEeVDVLDPETMKPVPrdGKtIGEIVFRGNIVMKGYLKNPEA 359
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 454 TARAiMADGFFRTGDMGFMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVAAEHPGVLESAAVGIPNEHSGEVVKLYVVR 533
Cdd:cd12118 360 TAEA-FRGGWFHSGDLAVIHPDGYIEIKDRSKDIIISGGENISSVEVEGVLYKHPAVLEAAVVARPDEKWGEVPCAFVEL 438
|
490 500 510 520
....*....|....*....|....*....|....*....|....*....
gi 479816261 534 RD-PELTEDEVKAFCAERLTNYKRPREVEFREsLPKSNVGKILRRELRD 581
Cdd:cd12118 439 KEgAKVTEEEIIAFCREHLAGFMVPKTVVFGE-LPKTSTGKIQKFVLRD 486
|
|
| PRK09088 |
PRK09088 |
acyl-CoA synthetase; Validated |
73-581 |
7.89e-65 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 181644 [Multi-domain] Cd Length: 488 Bit Score: 220.06 E-value: 7.89e-65
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 73 GKDISYKELDDHSRALAAWLQSRGLVKGDRVAIMMPNILQYPITFTAVLRAGFVVVNVNPLYTPRELEhqlndagakALV 152
Cdd:PRK09088 20 GRRWTYAELDALVGRLAAVLRRRGCVDGERLAVLARNSVWLVALHFACARVGAIYVPLNWRLSASELD---------ALL 90
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 153 vlenfattvEKALPSINVPNILVASMG-DMLGFKGHIVNLvvrrvkkmvpawnipghvrfkDALAQGRAKSFNPVPVqgc 231
Cdd:PRK09088 91 ---------QDAEPRLLLGDDAVAAGRtDVEDLAAFIASA---------------------DALEPADTPSIPPERV--- 137
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 232 diAFLQYTGGTTGISKGAMLTHSNILANVEQMNLWMDVAFRNKgkpkalnFVCALPLYHIFALTVNAMIGMKLGArNILI 311
Cdd:PRK09088 138 --SLILFTSGTSGQPKGVMLSERNLQQTAHNFGVLGRVDAHSS-------FLCDAPMFHIIGLITSVRPVLAVGG-SILV 207
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 312 PN---PRDIPSFVKELKKYPVHIFpGLNTLFNGLMNNPDFQTLDFKPLILTLGGGmaVQRPVAE-RWQHMTGCHITEGYG 387
Cdd:PRK09088 208 SNgfePKRTLGRLGDPALGITHYF-CVPQMAQAFRAQPGFDAAALRHLTALFTGG--APHAAEDiLGWLDDGIPMVDGFG 284
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 388 LSETSPVAcANALDATEF---TGTIGLPMPSTDVVIRDDDGSDLLLGKVGEICVRGPQVMKGYWNRPEETARAIMADGFF 464
Cdd:PRK09088 285 MSEAGTVF-GMSVDCDVIrakAGAAGIPTPTVQTRVVDDQGNDCPAGVPGELLLRGPNLSPGYWRRPQATARAFTGDGWF 363
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 465 RTGDMGFMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVAAEHPGVLESAAVGIPNEHSGEVVKLYVVRRDPELTE-DEV 543
Cdd:PRK09088 364 RTGDIARRDADGFFWVVDRKKDMFISGGENVYPAEIEAVLADHPGIRECAVVGMADAQWGEVGYLAIVPADGAPLDlERI 443
|
490 500 510
....*....|....*....|....*....|....*...
gi 479816261 544 KAFCAERLTNYKRPREVEFRESLPKSNVGKILRRELRD 581
Cdd:PRK09088 444 RSHLSTRLAKYKVPKHLRLVDALPRTASGKLQKARLRD 481
|
|
| PRK07787 |
PRK07787 |
acyl-CoA synthetase; Validated |
67-580 |
1.79e-64 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236096 [Multi-domain] Cd Length: 471 Bit Score: 218.32 E-value: 1.79e-64
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 67 PAFTCMGKDISYKELDDHSRALAAWLqsRGLvkgDRVAIMMPNILQYPITFTAVLRAGFVVVNVNPLYTPRELEHQLNDA 146
Cdd:PRK07787 17 DAVRIGGRVLSRSDLAGAATAVAERV--AGA---RRVAVLATPTLATVLAVVGALIAGVPVVPVPPDSGVAERRHILADS 91
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 147 GAKALvvlenfattvekalpsinvpniLVASMGDMLGFKGHIVNLVVRrvkkmvpAWNIPghvrfkdalaqgraksfnPV 226
Cdd:PRK07787 92 GAQAW----------------------LGPAPDDPAGLPHVPVRLHAR-------SWHRY------------------PE 124
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 227 PVQGCdIAFLQYTGGTTGISKGAMLTHSNILANVEQM-NLWM----DVafrnkgkpkalnFVCALPLYHIFALTVNAMIG 301
Cdd:PRK07787 125 PDPDA-PALIVYTSGTTGPPKGVVLSRRAIAADLDALaEAWQwtadDV------------LVHGLPLFHVHGLVLGVLGP 191
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 302 MKLGARniLIPNPRDIPSFVKELKKYPVHIFPGLNTLFNGLMNNPDfQTLDFKPLILTLGGGMAVQRPVAERWQHMTGCH 381
Cdd:PRK07787 192 LRIGNR--FVHTGRPTPEAYAQALSEGGTLYFGVPTVWSRIAADPE-AARALRGARLLVSGSAALPVPVFDRLAALTGHR 268
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 382 ITEGYGLSETSPVACANAlDATEFTGTIGLPMPSTDVVIRDDDGSDLLLG--KVGEICVRGPQVMKGYWNRPEETARAIM 459
Cdd:PRK07787 269 PVERYGMTETLITLSTRA-DGERRPGWVGLPLAGVETRLVDEDGGPVPHDgeTVGELQVRGPTLFDGYLNRPDATAAAFT 347
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 460 ADGFFRTGDMGFMDERGYTKIVDRKK-DMILVSGFNVYPNEIEEVAAEHPGVLESAAVGIPNEHSGEVVKLYVVRRDPeL 538
Cdd:PRK07787 348 ADGWFRTGDVAVVDPDGMHRIVGREStDLIKSGGYRIGAGEIETALLGHPGVREAAVVGVPDDDLGQRIVAYVVGADD-V 426
|
490 500 510 520
....*....|....*....|....*....|....*....|..
gi 479816261 539 TEDEVKAFCAERLTNYKRPREVEFRESLPKSNVGKILRRELR 580
Cdd:PRK07787 427 AADELIDFVAQQLSVHKRPREVRFVDALPRNAMGKVLKKQLL 468
|
|
| FAAL |
cd05931 |
Fatty acyl-AMP ligase (FAAL); FAAL belongs to the class I adenylate forming enzyme family and ... |
76-581 |
3.96e-64 |
|
Fatty acyl-AMP ligase (FAAL); FAAL belongs to the class I adenylate forming enzyme family and is homologous to fatty acyl-coenzyme A (CoA) ligases (FACLs). However, FAALs produce only the acyl adenylate and are unable to perform the thioester-forming reaction, while FACLs perform a two-step catalytic reaction; AMP ligation followed by CoA ligation using ATP and CoA as cofactors. FAALs have insertion motifs between the N-terminal and C-terminal subdomains that distinguish them from the FACLs. This insertion motif precludes the binding of CoA, thus preventing CoA ligation. It has been suggested that the acyl adenylates serve as substrates for multifunctional polyketide synthases to permit synthesis of complex lipids such as phthiocerol dimycocerosate, sulfolipids, mycolic acids, and mycobactin.
Pssm-ID: 341254 [Multi-domain] Cd Length: 547 Bit Score: 219.42 E-value: 3.96e-64
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 76 ISYKELDDHSRALAAWLQSRGLVkGDRVAIMMPNILQYPITFTAVLRAGFVVVnvnPLYTPRELEHQ------LNDAGAK 149
Cdd:cd05931 25 LTYAELDRRARAIAARLQAVGKP-GDRVLLLAPPGLDFVAAFLGCLYAGAIAV---PLPPPTPGRHAerlaaiLADAGPR 100
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 150 ALVVLENFATTVEKALPSinvpnilvasmgdmlgfkghivnlvvrrvkkmvPAWNIPGHVRFKDALAQGRAKSFNPVPVQ 229
Cdd:cd05931 101 VVLTTAAALAAVRAFAAS---------------------------------RPAAGTPRLLVVDLLPDTSAADWPPPSPD 147
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 230 GCDIAFLQYTGGTTGISKGAMLTHSNILANVEQMnlwmdvaFRNKGKPKALNFVCALPLYHIFALTVNAMIGMKLGARNI 309
Cdd:cd05931 148 PDDIAYLQYTSGSTGTPKGVVVTHRNLLANVRQI-------RRAYGLDPGDVVVSWLPLYHDMGLIGGLLTPLYSGGPSV 220
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 310 LIPnPRdipSFV-------KELKKYPVHIFPGlntlfnglmnnPDF--------------QTLDFKPLILTLGGGMAVQR 368
Cdd:cd05931 221 LMS-PA---AFLrrplrwlRLISRYRATISAA-----------PNFaydlcvrrvrdedlEGLDLSSWRVALNGAEPVRP 285
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 369 PVAERW-QHMTGCH-----ITEGYGLSE------TSPVA---CANALDATEFTGTI----------------GLPMPSTD 417
Cdd:cd05931 286 ATLRRFaEAFAPFGfrpeaFRPSYGLAEatlfvsGGPPGtgpVVLRVDRDALAGRAvavaaddpaarelvscGRPLPDQE 365
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 418 VVIRDDDGSDLLL-GKVGEICVRGPQVMKGYWNRPEETAR------AIMADGFFRTGDMGFMDErGYTKIVDRKKDMILV 490
Cdd:cd05931 366 VRIVDPETGRELPdGEVGEIWVRGPSVASGYWGRPEATAEtfgalaATDEGGWLRTGDLGFLHD-GELYITGRLKDLIIV 444
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 491 SGFNVYPNEIEEVAAEHPGVLES---AAVGIPNEHSGEVVKLYVVRRDP-ELTEDEVKAFCAERL-TNYK-RPREVEF-- 562
Cdd:cd05931 445 RGRNHYPQDIEATAEEAHPALRPgcvAAFSVPDDGEERLVVVAEVERGAdPADLAAIAAAIRAAVaREHGvAPADVVLvr 524
|
570
....*....|....*....
gi 479816261 563 RESLPKSNVGKILRRELRD 581
Cdd:cd05931 525 PGSIPRTSSGKIQRRACRA 543
|
|
| FACL_like_6 |
cd05922 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
84-580 |
4.10e-64 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341246 [Multi-domain] Cd Length: 457 Bit Score: 217.31 E-value: 4.10e-64
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 84 HSRALAAWlQSRGLVKGDRVAIMMPNILQYPITFTAVLRAG----FVVVNVNPLYTPRELEHQLNDAGAKalVVLenfat 159
Cdd:cd05922 3 VSAAASAL-LEAGGVRGERVVLILPNRFTYIELSFAVAYAGgrlgLVFVPLNPTLKESVLRYLVADAGGR--IVL----- 74
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 160 tvekalpsinvpniLVASMGDmlgfkghivnlvvrRVKKMVPAWNIPGHVRFKDALAqGRAKSFNPVPVQGCDIAFLQYT 239
Cdd:cd05922 75 --------------ADAGAAD--------------RLRDALPASPDPGTVLDADGIR-AARASAPAHEVSHEDLALLLYT 125
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 240 GGTTGISKGAMLTHSNILANVEQMNLWMDvafrNKGKPKALnfvCALPLYHIFALTVnAMIGMKLGARNILIPNPRDIPS 319
Cdd:cd05922 126 SGSTGSPKLVRLSHQNLLANARSIAEYLG----ITADDRAL---TVLPLSYDYGLSV-LNTHLLRGATLVLTNDGVLDDA 197
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 320 FVKELKKYPVHIFPGLNTLFNGLmnnpdfQTLDFKPL------ILTLGGGMAVQRPVAERWQHMTGCHITEGYGLSETSP 393
Cdd:cd05922 198 FWEDLREHGATGLAGVPSTYAML------TRLGFDPAklpslrYLTQAGGRLPQETIARLRELLPGAQVYVMYGQTEATR 271
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 394 -VACANALDATEFTGTIGLPMPSTDVVIRDDDGSDLLLGKVGEICVRGPQVMKGYWNRPEETARAIMADGFFRTGDMGFM 472
Cdd:cd05922 272 rMTYLPPERILEKPGSIGLAIPGGEFEILDDDGTPTPPGEPGEIVHRGPNVMKGYWNDPPYRRKEGRGGGVLHTGDLARR 351
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 473 DERGYTKIVDRKKDMILVSGFNVYPNEIEEVAAEHPGVLESAAVGIPNEhSGEVVKLYVVrRDPELTEDEVKAFCAERLT 552
Cdd:cd05922 352 DEDGFLFIVGRRDRMIKLFGNRISPTEIEAAARSIGLIIEAAAVGLPDP-LGEKLALFVT-APDKIDPKDVLRSLAERLP 429
|
490 500
....*....|....*....|....*...
gi 479816261 553 NYKRPREVEFRESLPKSNVGKILRRELR 580
Cdd:cd05922 430 PYKVPATVRVVDELPLTASGKVDYAALR 457
|
|
| MACS_euk |
cd05928 |
Eukaryotic Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step ... |
66-581 |
6.00e-64 |
|
Eukaryotic Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. The acyl-CoA is a key intermediate in many important biosynthetic and catabolic processes. MACS enzymes are localized to mitochondria. Two murine MACS family proteins are found in liver and kidney. In rodents, a MACS member is detected particularly in the olfactory epithelium and is called O-MACS. O-MACS demonstrates substrate preference for the fatty acid lengths of C6-C12.
Pssm-ID: 341251 [Multi-domain] Cd Length: 530 Bit Score: 218.87 E-value: 6.00e-64
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 66 SPAFTCM---GKDI--SYKELDDHSRALAAWL-QSRGLVKGDRVAIMMPNILQYPITFTAVLRAGFVVVNVNPLYTPREL 139
Cdd:cd05928 27 NPALWWVngkGDEVkwSFRELGSLSRKAANVLsGACGLQRGDRVAVILPRVPEWWLVNVACIRTGLVFIPGTIQLTAKDI 106
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 140 EHQLNDAGAKALVVLENFATTVEKAlpSINVPNIlvasmgdmlgfkghivnlvvrRVKKMVPAWNIPGHVRFKDaLAQGR 219
Cdd:cd05928 107 LYRLQASKAKCIVTSDELAPEVDSV--ASECPSL---------------------KTKLLVSEKSRDGWLNFKE-LLNEA 162
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 220 AKSFNPVPVQGCDIAFLQYTGGTTGISKGAMLTHSNI-LANVEQMNLWM-----DVAFRNKGKPKALNFVCALplyhiFA 293
Cdd:cd05928 163 STEHHCVETGSQEPMAIYFTSGTTGSPKMAEHSHSSLgLGLKVNGRYWLdltasDIMWNTSDTGWIKSAWSSL-----FE 237
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 294 LTVNamigmklGARNILIPNPR-DIPSFVKELKKYPVHIFPGLNTLFNGLMNNpDFQTLDFKPLILTLGGGMAVQRPVAE 372
Cdd:cd05928 238 PWIQ-------GACVFVHHLPRfDPLVILKTLSSYPITTFCGAPTVYRMLVQQ-DLSSYKFPSLQHCVTGGEPLNPEVLE 309
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 373 RWQHMTGCHITEGYGLSETSpVACANALDATEFTGTIGLPMPSTDVVIRDDDGSDLLLGKVGEICVR-GPQ----VMKGY 447
Cdd:cd05928 310 KWKAQTGLDIYEGYGQTETG-LICANFKGMKIKPGSMGKASPPYDVQIIDDNGNVLPPGTEGDIGIRvKPIrpfgLFSGY 388
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 448 WNRPEETARAIMADgFFRTGDMGFMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVAAEHPGVLESAAVGIPNEHSGEVV 527
Cdd:cd05928 389 VDNPEKTAATIRGD-FYLTGDRGIMDEDGYFWFMGRADDVINSSGYRIGPFEVESALIEHPAVVESAVVSSPDPIRGEVV 467
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....
gi 479816261 528 KLYVV------RRDPE-LT---EDEVKAFCAErltnYKRPREVEFRESLPKSNVGKILRRELRD 581
Cdd:cd05928 468 KAFVVlapqflSHDPEqLTkelQQHVKSVTAP----YKYPRKVEFVQELPKTVTGKIQRNELRD 527
|
|
| 23DHB-AMP_lg |
cd05920 |
2,3-dihydroxybenzoate-AMP ligase; 2,3-dihydroxybenzoate-AMP ligase activates 2, ... |
51-579 |
9.27e-64 |
|
2,3-dihydroxybenzoate-AMP ligase; 2,3-dihydroxybenzoate-AMP ligase activates 2,3-dihydroxybenzoate (DHB) by ligation of AMP from ATP with the release of pyrophosphate. However, it can also catalyze the ATP-PPi exchange for 2,3-DHB analogs, such as salicyclic acid (o-hydrobenzoate), as well as 2,4-DHB and 2,5-DHB, but with less efficiency. Proteins in this family are the stand-alone adenylation components of non-ribosomal peptide synthases (NRPSs) involved in the biosynthesis of siderophores, which are low molecular weight iron-chelating compounds synthesized by many bacteria to aid in the acquisition of this vital trace elements. In Escherichia coli, the 2,3-dihydroxybenzoate-AMP ligase is called EntE, the adenylation component of the enterobactin NRPS system.
Pssm-ID: 341244 [Multi-domain] Cd Length: 482 Bit Score: 216.81 E-value: 9.27e-64
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 51 SISDMISASCRQFANSPAFTCMGKDISYKELDDHSRALAAWLQSRGLVKGDRVAIMMPNILQYPITFTAVLRAGFVVVNV 130
Cdd:cd05920 16 PLGDLLARSAARHPDRIAVVDGDRRLTYRELDRRADRLAAGLRGLGIRPGDRVVVQLPNVAEFVVLFFALLRLGAVPVLA 95
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 131 NPLYTPRELEHQLNDAGAKALVVLENFAttvekalpsinvpnilvasmgdmlGFKGHivnlvvrrvkkmvpawnipghvr 210
Cdd:cd05920 96 LPSHRRSELSAFCAHAEAVAYIVPDRHA------------------------GFDHR----------------------- 128
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 211 fkdALAQGRAKSFNpvpvqgcDIAFLQYTGGTTGISKGAMLTHS----NILANVEQMNLWMDVAFrnkgkpkalnfVCAL 286
Cdd:cd05920 129 ---ALARELAESIP-------EVALFLLSGGTTGTPKLIPRTHNdyayNVRASAEVCGLDQDTVY-----------LAVL 187
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 287 PLYHIFALTVNAMIG-MKLGARNILIPNPRdipsfvkelkkyPVHIFP-------GLNTLFNGL----MNNPDFQTLDFK 354
Cdd:cd05920 188 PAAHNFPLACPGVLGtLLAGGRVVLAPDPS------------PDAAFPlieregvTVTALVPALvslwLDAAASRRADLS 255
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 355 PLILTLGGGMAVQRPVAERWQHMTGCHITEGYGLSETspVACANALDATEFT--GTIGLPM-PSTDVVIRDDDGSDLLLG 431
Cdd:cd05920 256 SLRLLQVGGARLSPALARRVPPVLGCTLQQVFGMAEG--LLNYTRLDDPDEViiHTQGRPMsPDDEIRVVDEEGNPVPPG 333
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 432 KVGEICVRGPQVMKGYWNRPEETARAIMADGFFRTGDMGFMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVAAEHPGVL 511
Cdd:cd05920 334 EEGELLTRGPYTIRGYYRAPEHNARAFTPDGFYRTGDLVRRTPDGYLVVEGRIKDQINRGGEKIAAEEVENLLLRHPAVH 413
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 479816261 512 ESAAVGIPNEHSGEVVKLYVVRRDPELTEDEVKAFCAER-LTNYKRPREVEFRESLPKSNVGKILRREL 579
Cdd:cd05920 414 DAAVVAMPDELLGERSCAFVVLRDPPPSAAQLRRFLRERgLAAYKLPDRIEFVDSLPLTAVGKIDKKAL 482
|
|
| PRK13391 |
PRK13391 |
acyl-CoA synthetase; Provisional |
73-581 |
7.66e-63 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 184022 [Multi-domain] Cd Length: 511 Bit Score: 215.33 E-value: 7.66e-63
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 73 GKDISYKELDDHSRALAAWLQSRGLVKGDRVAIMMPNILQYPITFTAVLRAGFVVVNVNPLYTPRELEHQLNDAGAKALV 152
Cdd:PRK13391 22 GEVVTYRELDERSNRLAHLFRSLGLKRGDHVAIFMENNLRYLEVCWAAERSGLYYTCVNSHLTPAEAAYIVDDSGARALI 101
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 153 VLENFATTVEKALPsiNVPNILVASMGDMLGfkghivnlvvrrvkkmvpawNIPGHVRFKDALAQGRAksfNPVP--VQG 230
Cdd:PRK13391 102 TSAAKLDVARALLK--QCPGVRHRLVLDGDG--------------------ELEGFVGYAEAVAGLPA---TPIAdeSLG 156
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 231 CDiafLQYTGGTTGISKG--AMLTHSNIlanVEQMNLWMdVAFRNKGKPKALNFVCALPLYHIFALTVNaMIGMKLGARN 308
Cdd:PRK13391 157 TD---MLYSSGTTGRPKGikRPLPEQPP---DTPLPLTA-FLQRLWGFRSDMVYLSPAPLYHSAPQRAV-MLVIRLGGTV 228
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 309 ILIPNpRDIPSFVKELKKYPV---HIFPglnTLFNGLMNNPDFQ--TLDFKPLILTLGGGMA----VQRPVAERWqhmtG 379
Cdd:PRK13391 229 IVMEH-FDAEQYLALIEEYGVthtQLVP---TMFSRMLKLPEEVrdKYDLSSLEVAIHAAAPcppqVKEQMIDWW----G 300
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 380 CHITEGYGLSETSPVACANALDATEFTGTIGLPMPStDVVIRDDDGSDLLLGKVGEICVRGPQVMKgYWNRPEETARAIM 459
Cdd:PRK13391 301 PIIHEYYAATEGLGFTACDSEEWLAHPGTVGRAMFG-DLHILDDDGAELPPGEPGTIWFEGGRPFE-YLNDPAKTAEARH 378
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 460 ADGFFRT-GDMGFMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVAAEHPGVLESAAVGIPNEHSGEVVKLYV-----VR 533
Cdd:PRK13391 379 PDGTWSTvGDIGYVDEDGYLYLTDRAAFMIISGGVNIYPQEAENLLITHPKVADAAVFGVPNEDLGEEVKAVVqpvdgVD 458
|
490 500 510 520
....*....|....*....|....*....|....*....|....*...
gi 479816261 534 RDPELTEdEVKAFCAERLTNYKRPREVEFRESLPKSNVGKILRRELRD 581
Cdd:PRK13391 459 PGPALAA-ELIAFCRQRLSRQKCPRSIDFEDELPRLPTGKLYKRLLRD 505
|
|
| ACLS-CaiC |
cd17637 |
acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II; This family contains fatty acid CoA ... |
238-576 |
8.55e-63 |
|
acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II; This family contains fatty acid CoA ligases, including acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II, most of which are yet to be characterized, but may be similar to Carnitine-CoA ligase (CaiC) which catalyzes the transfer of CoA to carnitine. Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341292 [Multi-domain] Cd Length: 333 Bit Score: 209.82 E-value: 8.55e-63
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 238 YTGGTTGISKGAMLTHSNILANVEQMNLWMDVAfrnkGKPKALNfvcALPLYHIFALTVnAMIGMKLGARNILIPNpRDI 317
Cdd:cd17637 7 HTAAVAGRPRGAVLSHGNLIAANLQLIHAMGLT----EADVYLN---MLPLFHIAGLNL-ALATFHAGGANVVMEK-FDP 77
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 318 PSFVKELKKYPVHIF----PGLNTLFNGLMNNPdfqtLDFKPLILTLGggmaVQRP-VAERWQHMTGCHITEGYGLSETS 392
Cdd:cd17637 78 AEALELIEEEKVTLMgsfpPILSNLLDAAEKSG----VDLSSLRHVLG----LDAPeTIQRFEETTGATFWSLYGQTETS 149
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 393 PVACANalDATEFTGTIGLPMPSTDVVIRDDDGSDLLLGKVGEICVRGPQVMKGYWNRPEETARAiMADGFFRTGDMGFM 472
Cdd:cd17637 150 GLVTLS--PYRERPGSAGRPGPLVRVRIVDDNDRPVPAGETGEIVVRGPLVFQGYWNLPELTAYT-FRNGWHHTGDLGRF 226
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 473 DERGYTKIVDRK--KDMILVSGFNVYPNEIEEVAAEHPGVLESAAVGIPNEHSGEVVKLYVVRRDPE-LTEDEVKAFCAE 549
Cdd:cd17637 227 DEDGYLWYAGRKpeKELIKPGGENVYPAEVEKVILEHPAIAEVCVIGVPDPKWGEGIKAVCVLKPGAtLTADELIEFVGS 306
|
330 340
....*....|....*....|....*..
gi 479816261 550 RLTNYKRPREVEFRESLPKSNVGKILR 576
Cdd:cd17637 307 RIARYKKPRYVVFVEALPKTADGSIDR 333
|
|
| MACS_like_3 |
cd05971 |
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the ... |
74-581 |
2.41e-62 |
|
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. MACS enzymes are localized to mitochondria.
Pssm-ID: 341275 [Multi-domain] Cd Length: 439 Bit Score: 211.91 E-value: 2.41e-62
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 74 KDISYKELDDHSRALAAWLQSRGLVKGDRVAIMMPNILQYPITFTAVLRAGFVVVNVNPLYTPRELEHQLNDAGAKALVV 153
Cdd:cd05971 5 EKVTFKELKTASNRFANVLKEIGLEKGDRVGVFLSQGPECAIAHIAILRSGAIAVPLFALFGPEALEYRLSNSGASALVT 84
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 154 lenfattvekalpsiNVPNilvasmgdmlgfkghivnlvvrrvkkmvpawnipghvrfkdalaqgraksfnpvpvqgcDI 233
Cdd:cd05971 85 ---------------DGSD-----------------------------------------------------------DP 90
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 234 AFLQYTGGTTGISKGAMLTHSNILANVEQMNLWMDVAFRNK---------GKPKALnFVCALPLYHiFALTV-------- 296
Cdd:cd05971 91 ALIIYTSGTTGPPKGALHAHRVLLGHLPGVQFPFNLFPRDGdlywtpadwAWIGGL-LDVLLPSLY-FGVPVlahrmtkf 168
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 297 ---NAMIGMK-LGARNILIPnprdiPSFVKELKKYPVHIFPGLNTLfnglmnnpdfqtldfkpLILTLGGGMAVQRPVAE 372
Cdd:cd05971 169 dpkAALDLMSrYGVTTAFLP-----PTALKMMRQQGEQLKHAQVKL-----------------RAIATGGESLGEELLGW 226
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 373 RWQHMtGCHITEGYGLSETSPVACANALDATEFTGTIGLPMPSTDVVIRDDDGSDLLLGKVGEICVRGPQ--VMKGYWNR 450
Cdd:cd05971 227 AREQF-GVEVNEFYGQTECNLVIGNCSALFPIKPGSMGKPIPGHRVAIVDDNGTPLPPGEVGEIAVELPDpvAFLGYWNN 305
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 451 PEETaRAIMADGFFRTGDMGFMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVAAEHPGVLESAAVGIPNEHSGEVVKLY 530
Cdd:cd05971 306 PSAT-EKKMAGDWLLTGDLGRKDSDGYFWYVGRDDDVITSSGYRIGPAEIEECLLKHPAVLMAAVVGIPDPIRGEIVKAF 384
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*
gi 479816261 531 VVRRDPELTED----EVKAFCAERLTNYKRPREVEFRESLPKSNVGKILRRELRD 581
Cdd:cd05971 385 VVLNPGETPSDalarEIQELVKTRLAAHEYPREIEFVNELPRTATGKIRRRELRA 439
|
|
| Firefly_Luc |
cd17642 |
insect luciferase, similar to plant 4-coumarate: CoA ligases; This family contains insect ... |
62-581 |
3.28e-61 |
|
insect luciferase, similar to plant 4-coumarate: CoA ligases; This family contains insect firefly luciferases that share significant sequence similarity to plant 4-coumarate:coenzyme A ligases, despite their functional diversity. Luciferase catalyzes the production of light in the presence of MgATP, molecular oxygen, and luciferin. In the first step, luciferin is activated by acylation of its carboxylate group with ATP, resulting in an enzyme-bound luciferyl adenylate. In the second step, luciferyl adenylate reacts with molecular oxygen, producing an enzyme-bound excited state product (Luc=O*) and releasing AMP. This excited-state product then decays to the ground state (Luc=O), emitting a quantum of visible light.
Pssm-ID: 341297 [Multi-domain] Cd Length: 532 Bit Score: 211.62 E-value: 3.28e-61
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 62 QFANSPAFT--CMGKDISYKELDDHSRALAAWLQSRGLVKGDRVAIMMPNILQYPITFTAVLRAGFVVVNVNPLYTPREL 139
Cdd:cd17642 29 SVPGTIAFTdaHTGVNYSYAEYLEMSVRLAEALKKYGLKQNDRIAVCSENSLQFFLPVIAGLFIGVGVAPTNDIYNEREL 108
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 140 EHQLNDAGAKALVVLENF---ATTVEKALPSINVPNILvASMGDMLGFKghivnlvvrRVKKMVPAWNIPGHvrfkdala 216
Cdd:cd17642 109 DHSLNISKPTIVFCSKKGlqkVLNVQKKLKIIKTIIIL-DSKEDYKGYQ---------CLYTFITQNLPPGF-------- 170
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 217 qgRAKSFNPVPV-QGCDIAFLQYTGGTTGISKGAMLTHSNILANVEQMnlwMDVAFRNKGKPKAlNFVCALPLYHIFALT 295
Cdd:cd17642 171 --NEYDFKPPSFdRDEQVALIMNSSGSTGLPKGVQLTHKNIVARFSHA---RDPIFGNQIIPDT-AILTVIPFHHGFGMF 244
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 296 vnAMIG-MKLGARNILIPNpRDIPSFVKELKKYPVHIFPGLNTLFNGLMNNPDFQTLDFKPLILTLGGGMAVQRP----V 370
Cdd:cd17642 245 --TTLGyLICGFRVVLMYK-FEEELFLRSLQDYKVQSALLVPTLFAFFAKSTLVDKYDLSNLHEIASGGAPLSKEvgeaV 321
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 371 AERWqHMTGchITEGYGLSE-TSPVACANalDATEFTGTIGLPMPSTDV-VIRDDDGSDLLLGKVGEICVRGPQVMKGYW 448
Cdd:cd17642 322 AKRF-KLPG--IRQGYGLTEtTSAILITP--EGDDKPGAVGKVVPFFYAkVVDLDTGKTLGPNERGELCVKGPMIMKGYV 396
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 449 NRPEETARAIMADGFFRTGDMGFMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVAAEHPGVLESAAVGIPNEHSGEVVK 528
Cdd:cd17642 397 NNPEATKALIDKDGWLHSGDIAYYDEDGHFFIVDRLKSLIKYKGYQVPPAELESILLQHPKIFDAGVAGIPDEDAGELPA 476
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*
gi 479816261 529 LYVVRR-DPELTEDEVKAFCAERLTNYKRPR-EVEFRESLPKSNVGKILRRELRD 581
Cdd:cd17642 477 AVVVLEaGKTMTEKEVMDYVASQVSTAKRLRgGVKFVDEVPKGLTGKIDRRKIRE 531
|
|
| LC_FACL_like |
cd05914 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); The members of this family are ... |
73-576 |
9.00e-61 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); The members of this family are bacterial long-chain fatty acid CoA synthetase, most of which are as yet uncharacterized. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.
Pssm-ID: 341240 [Multi-domain] Cd Length: 463 Bit Score: 208.45 E-value: 9.00e-61
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 73 GKDISYKELDDHSRALAAWLQSRGLVKGDRVAIMMPNILQYPITFTAVLRAGFVVVNVNPLYTPRELEHQLNDAGAKALV 152
Cdd:cd05914 5 GEPLTYKDLADNIAKFALLLKINGVGTGDRVALMGENRPEWGIAFFAIWTYGAIAVPILAEFTADEVHHILNHSEAKAIF 84
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 153 VLENFattvekalpsinvpnilvasmgdmlgfkghivnlvvrrvkkmvpawnipghvrfkdalaqgraksfnpvpvqgcD 232
Cdd:cd05914 85 VSDED--------------------------------------------------------------------------D 90
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 233 IAFLQYTGGTTGISKGAMLTHSNILANVEqmnlwmdvAFRNKGKPKALNFVCA-LPLYHIFALTVNAMIGMKLGARNIL- 310
Cdd:cd05914 91 VALINYTSGTTGNSKGVMLTYRNIVSNVD--------GVKEVVLLGKGDKILSiLPLHHIYPLTFTLLLPLLNGAHVVFl 162
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 311 --IPNPR-DIPSFVKE------------LKKYPVHIFPGLNT-----LFNGLMNNPDFQTLDFKPLILTLGG-------G 363
Cdd:cd05914 163 dkIPSAKiIALAFAQVtptlgvpvplviEKIFKMDIIPKLTLkkfkfKLAKKINNRKIRKLAFKKVHEAFGGnikefviG 242
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 364 MAVQRPVAERWQHMTGCHITEGYGLSETSPVACANALDATEFTGTiGLPMPSTDVVIRDDDGSdlllGKVGEICVRGPQV 443
Cdd:cd05914 243 GAKINPDVEEFLRTIGFPYTIGYGMTETAPIISYSPPNRIRLGSA-GKVIDGVEVRIDSPDPA----TGEGEIIVRGPNV 317
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 444 MKGYWNRPEETARAIMADGFFRTGDMGFMDERGYTKIVDRKKDMILV-SGFNVYPNEIEEVAAEHPGVLESAavgIPNEH 522
Cdd:cd05914 318 MKGYYKNPEATAEAFDKDGWFHTGDLGKIDAEGYLYIRGRKKEMIVLsSGKNIYPEEIEAKINNMPFVLESL---VVVQE 394
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 479816261 523 SGEVVKLYV-----------VRRDPELTEDEVKAFCAERLTNYKRPREV-EFRESLPKSNVGKILR 576
Cdd:cd05914 395 KKLVALAYIdpdfldvkalkQRNIIDAIKWEVRDKVNQKVPNYKKISKVkIVKEEFEKTPKGKIKR 460
|
|
| A_NRPS |
cd05930 |
The adenylation domain of nonribosomal peptide synthetases (NRPS); The adenylation (A) domain ... |
64-579 |
2.74e-60 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS); The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341253 [Multi-domain] Cd Length: 444 Bit Score: 206.61 E-value: 2.74e-60
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 64 ANSPAFTCMGKDISYKELDDHSRALAAWLQSRGLVKGDRVAIMMPNILQYPITFTAVLRAGFVVVNVNPLYtPRE-LEHQ 142
Cdd:cd05930 1 PDAVAVVDGDQSLTYAELDARANRLARYLRERGVGPGDLVAVLLERSLEMVVAILAVLKAGAAYVPLDPSY-PAErLAYI 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 143 LNDAGAKALVVlenfattvekalpsinvpnilvasmgdmlgfkghivnlvvrrvkkmvpawnipghvrfkdalaqgraks 222
Cdd:cd05930 80 LEDSGAKLVLT--------------------------------------------------------------------- 90
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 223 fnpvpvQGCDIAFLQYTGGTTGISKGAMLTHSNILANVeqmnLWMDVAFRNKGKPKALNFVcalPLyhIFALTVNAMIGM 302
Cdd:cd05930 91 ------DPDDLAYVIYTSGSTGKPKGVMVEHRGLVNLL----LWMQEAYPLTPGDRVLQFT---SF--SFDVSVWEIFGA 155
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 303 KL-GARNILIPN--PRDIPSFVKELKKYPV---HIFPglnTLFNGLMNNPDFQtlDFKPLILTLGGGMAVQRPVAERW-Q 375
Cdd:cd05930 156 LLaGATLVVLPEevRKDPEALADLLAEEGItvlHLTP---SLLRLLLQELELA--ALPSLRLVLVGGEALPPDLVRRWrE 230
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 376 HMTGCHITEGYGLSETSPVACANALDATEFTG---TIGLPMPSTDVVIRDDDGSDLLLGKVGEICVRGPQVMKGYWNRPE 452
Cdd:cd05930 231 LLPGARLVNLYGPTEATVDATYYRVPPDDEEDgrvPIGRPIPNTRVYVLDENLRPVPPGVPGELYIGGAGLARGYLNRPE 310
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 453 ETARAIMADGFF------RTGDMGFMDERG---YtkiVDRKKDMILVSGFNVYPNEIEEVAAEHPGVLESAAVGIPNEHS 523
Cdd:cd05930 311 LTAERFVPNPFGpgermyRTGDLVRWLPDGnleF---LGRIDDQVKIRGYRIELGEIEAALLAHPGVREAAVVAREDGDG 387
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*..
gi 479816261 524 GEVVKLYVV-RRDPELTEDEVKAFCAERLTNYKRPREVEFRESLPKSNVGKILRREL 579
Cdd:cd05930 388 EKRLVAYVVpDEGGELDEEELRAHLAERLPDYMVPSAFVVLDALPLTPNGKVDRKAL 444
|
|
| ABCL |
cd05958 |
2-aminobenzoate-CoA ligase (ABCL); ABCL catalyzes the initial step in the 2-aminobenzoate ... |
67-580 |
3.20e-60 |
|
2-aminobenzoate-CoA ligase (ABCL); ABCL catalyzes the initial step in the 2-aminobenzoate aerobic degradation pathway by activating 2-aminobenzoate to 2-aminobenzoyl-CoA. The reaction is carried out via a two-step process; the first step is ATP-dependent and forms a 2-aminobenzoyl-AMP intermediate, and the second step forms the 2-aminobenzoyl-CoA ester and releases the AMP. 2-Aminobenzoyl-CoA is further converted to 2-amino-5-oxo-cyclohex-1-ene-1-carbonyl-CoA catalyzed by 2-aminobenzoyl-CoA monooxygenase/reductase. ABCL has been purified from cells aerobically grown with 2-aminobenzoate as sole carbon, energy, and nitrogen source, and has been characterized as a monomer.
Pssm-ID: 341268 [Multi-domain] Cd Length: 439 Bit Score: 206.17 E-value: 3.20e-60
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 67 PAFTCMGKDISYKELDDHSRALAAWLQSR-GLVKGDRVAIMMPNILQYPITFTAVLRAGFVVVNVNPLYTPRELEHQLND 145
Cdd:cd05958 2 TCLRSPEREWTYRDLLALANRIANVLVGElGIVPGNRVLLRGSNSPELVACWFGIQKAGAIAVATMPLLRPKELAYILDK 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 146 AgAKALVVLENFATTVEkalpsinvpnilvasmgdmlgfkghivnlvvrrvkkmvpawnipghvrfkdalaqgraksfnp 225
Cdd:cd05958 82 A-RITVALCAHALTASD--------------------------------------------------------------- 97
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 226 vpvqgcDIAFLQYTGGTTGISKGAMLTHSNILANVEqmnLWMdvafRNKGKPKALNFVCALP-LYHIFALTVNAMIGMKL 304
Cdd:cd05958 98 ------DICILAFTSGTTGAPKATMHFHRDPLASAD---RYA----VNVLRLREDDRFVGSPpLAFTFGLGGVLLFPFGV 164
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 305 GARNILIPN--PRDIPSFVKElkkYPVHIFPGLNTLFNGLMNNPDFQTLDFKPLILTLGGGMAVQRPVAERWQHMTGCHI 382
Cdd:cd05958 165 GASGVLLEEatPDLLLSAIAR---YKPTVLFTAPTAYRAMLAHPDAAGPDLSSLRKCVSAGEALPAALHRAWKEATGIPI 241
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 383 TEGYGLSETSPVACANALDATEfTGTIGLPMPSTDVVIRDDDGSDLLLGKVGEICVRGPqvmKGYWNRPEETARAIMADG 462
Cdd:cd05958 242 IDGIGSTEMFHIFISARPGDAR-PGATGKPVPGYEAKVVDDEGNPVPDGTIGRLAVRGP---TGCRYLADKRQRTYVQGG 317
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 463 FFRTGDMGFMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVAAEHPGVLESAAVGIPNEHSGEVVKLYVVRR-----DPE 537
Cdd:cd05958 318 WNITGDTYSRDPDGYFRHQGRSDDMIVSGGYNIAPPEVEDVLLQHPAVAECAVVGHPDESRGVVVKAFVVLRpgvipGPV 397
|
490 500 510 520
....*....|....*....|....*....|....*....|...
gi 479816261 538 LTEdEVKAFCAERLTNYKRPREVEFRESLPKSNVGKILRRELR 580
Cdd:cd05958 398 LAR-ELQDHAKAHIAPYKYPRAIEFVTELPRTATGKLQRFALR 439
|
|
| PRK13295 |
PRK13295 |
cyclohexanecarboxylate-CoA ligase; Reviewed |
52-581 |
3.24e-59 |
|
cyclohexanecarboxylate-CoA ligase; Reviewed
Pssm-ID: 171961 [Multi-domain] Cd Length: 547 Bit Score: 206.44 E-value: 3.24e-59
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 52 ISDMISASCRQFANSPAFTCMGKD------ISYKELDDHSRALAAWLQSRGLVKGDRVAIMMPNILQYPITFTAVLRAGF 125
Cdd:PRK13295 26 INDDLDACVASCPDKTAVTAVRLGtgaprrFTYRELAALVDRVAVGLARLGVGRGDVVSCQLPNWWEFTVLYLACSRIGA 105
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 126 VVVNVNPLYTPRELEHQLNDAGAKALVVLENF--------ATTVEKALPSINvpNILVASMGDMLGFKGHIvnlvvrrvk 197
Cdd:PRK13295 106 VLNPLMPIFRERELSFMLKHAESKVLVVPKTFrgfdhaamARRLRPELPALR--HVVVVGGDGADSFEALL--------- 174
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 198 kMVPAWNipghvrfKDALAQGRAKSFNPVPVqgcDIAFLQYTGGTTGISKGAMLTH----SNILANVEQMNLWM-DVAFR 272
Cdd:PRK13295 175 -ITPAWE-------QEPDAPAILARLRPGPD---DVTQLIYTSGTTGEPKGVMHTAntlmANIVPYAERLGLGAdDVILM 243
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 273 nkgkpkalnfvcALPLYHIFALTVNAMIGMKLGARNIL--IPNPRdipSFVKELKKYPVHIFPGLNTLFNGLMNNPDFQT 350
Cdd:PRK13295 244 ------------ASPMAHQTGFMYGLMMPVMLGATAVLqdIWDPA---RAAELIRTEGVTFTMASTPFLTDLTRAVKESG 308
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 351 LDFKPLILTLGGGMAVQRPVAERWQHMTGCHITEGYGLSETSPVACANALDATE-FTGTIGLPMPSTDVVIRDDDGSDLL 429
Cdd:PRK13295 309 RPVSSLRTFLCAGAPIPGALVERARAALGAKIVSAWGMTENGAVTLTKLDDPDErASTTDGCPLPGVEVRVVDADGAPLP 388
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 430 LGKVGEICVRGPQVMKGYWNRPEETARAimADGFFRTGDMGFMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVAAEHPG 509
Cdd:PRK13295 389 AGQIGRLQVRGCSNFGGYLKRPQLNGTD--ADGWFDTGDLARIDADGYIRISGRSKDVIIRGGENIPVVEIEALLYRHPA 466
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 479816261 510 VLESAAVGIPNEHSGEVVKLYVVRRDPE-LTEDEVKAFC-AERLTNYKRPREVEFRESLPKSNVGKI----LRRELRD 581
Cdd:PRK13295 467 IAQVAIVAYPDERLGERACAFVVPRPGQsLDFEEMVEFLkAQKVAKQYIPERLVVRDALPRTPSGKIqkfrLREMLRG 544
|
|
| PRK07788 |
PRK07788 |
acyl-CoA synthetase; Validated |
76-581 |
1.14e-58 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236097 [Multi-domain] Cd Length: 549 Bit Score: 204.78 E-value: 1.14e-58
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 76 ISYKELDDHSRALAAWLQSRGLVKGDRVAIMMPNILQYPITFTAVLRAGFVVVNVNPLYTPRELEHQLNDAGAKALVVLE 155
Cdd:PRK07788 75 LTYAELDEQSNALARGLLALGVRAGDGVAVLARNHRGFVLALYAAGKVGARIILLNTGFSGPQLAEVAAREGVKALVYDD 154
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 156 NFATTVEKALPSinvpnilvasMGDMLGFKGHivnlvVRRVKKMVPAWnipghvRFKDALAQGRAKSFNPVPVQGCDIAF 235
Cdd:PRK07788 155 EFTDLLSALPPD----------LGRLRAWGGN-----PDDDEPSGSTD------ETLDDLIAGSSTAPLPKPPKPGGIVI 213
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 236 LqyTGGTTGISKGAMLTHSNILANVEQMNLWmdVAFRNKGkpkalNFVCALPLYHIFALtVNAMIGMKLGARNIL--IPN 313
Cdd:PRK07788 214 L--TSGTTGTPKGAPRPEPSPLAPLAGLLSR--VPFRAGE-----TTLLPAPMFHATGW-AHLTLAMALGSTVVLrrRFD 283
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 314 P----RDI-----------PSFVKELKKYPVHIfpglntlfnglMNNPDFQTLDFkplilTLGGGMAVQRPVAERWQHMT 378
Cdd:PRK07788 284 PeatlEDIakhkatalvvvPVMLSRILDLGPEV-----------LAKYDTSSLKI-----IFVSGSALSPELATRALEAF 347
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 379 GCHITEGYGLSETSPVACANALDATEFTGTIGLPMPSTDVVIRDDDGSDLLLGKVGEICVRGPQVMKGYWN-RPEETAra 457
Cdd:PRK07788 348 GPVLYNLYGSTEVAFATIATPEDLAEAPGTVGRPPKGVTVKILDENGNEVPRGVVGRIFVGNGFPFEGYTDgRDKQII-- 425
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 458 imaDGFFRTGDMGFMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVAAEHPGVLESAAVGIPNEHSGEVVKLYVVRRDPE 537
Cdd:PRK07788 426 ---DGLLSSGDVGYFDEDGLLFVDGRDDDMIVSGGENVFPAEVEDLLAGHPDVVEAAVIGVDDEEFGQRLRAFVVKAPGA 502
|
490 500 510 520
....*....|....*....|....*....|....*....|....*
gi 479816261 538 -LTEDEVKAFCAERLTNYKRPREVEFRESLPKSNVGKILRRELRD 581
Cdd:PRK07788 503 aLDEDAIKDYVRDNLARYKVPRDVVFLDELPRNPTGKVLKRELRE 547
|
|
| FACL_like_4 |
cd05944 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
232-580 |
4.62e-58 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341266 [Multi-domain] Cd Length: 359 Bit Score: 198.09 E-value: 4.62e-58
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 232 DIAFLQYTGGTTGISKGAMLTHSNILANVEQMNLWMDVafrnkgKPKAlNFVCALPLYHIFALTVNAMIGMKLGArNILI 311
Cdd:cd05944 3 DVAAYFHTGGTTGTPKLAQHTHSNEVYNAWMLALNSLF------DPDD-VLLCGLPLFHVNGSVVTLLTPLASGA-HVVL 74
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 312 PNP---RD---IPSFVKELKKYPVHIFPGLNTLFNGLMNNPDfqTLDFKPLILTLGGGMAVQRPVAERWQHMTGCHITEG 385
Cdd:cd05944 75 AGPagyRNpglFDNFWKLVERYRITSLSTVPTVYAALLQVPV--NADISSLRFAMSGAAPLPVELRARFEDATGLPVVEG 152
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 386 YGLSETSPVACANALDATEFTGTIGLPMPSTDVVIRDDDGSDLLL-----GKVGEICVRGPQVMKGYWNRpEETARAIMA 460
Cdd:cd05944 153 YGLTEATCLVAVNPPDGPKRPGSVGLRLPYARVRIKVLDGVGRLLrdcapDEVGEICVAGPGVFGGYLYT-EGNKNAFVA 231
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 461 DGFFRTGDMGFMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVAAEHPGVLESAAVGIPNEHSGEVVKLYV-VRRDPELT 539
Cdd:cd05944 232 DGWLNTGDLGRLDADGYLFITGRAKDLIIRGGHNIDPALIEEALLRHPAVAFAGAVGQPDAHAGELPVAYVqLKPGAVVE 311
|
330 340 350 360
....*....|....*....|....*....|....*....|..
gi 479816261 540 EDEVKAFCAERLTNYKR-PREVEFRESLPKSNVGKILRRELR 580
Cdd:cd05944 312 EEELLAWARDHVPERAAvPKHIEVLEELPVTAVGKVFKPALR 353
|
|
| caiC |
PRK08008 |
putative crotonobetaine/carnitine-CoA ligase; Validated |
77-580 |
5.93e-58 |
|
putative crotonobetaine/carnitine-CoA ligase; Validated
Pssm-ID: 181195 [Multi-domain] Cd Length: 517 Bit Score: 202.22 E-value: 5.93e-58
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 77 SYKELDDHSRALAAWLQSRGLVKGDRVAIMMPNILQYPITFTAVLRAGFVVVNVNPLYTPRELEHQLNDAGAKALVVLEN 156
Cdd:PRK08008 39 SYLELNEEINRTANLFYSLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESAWILQNSQASLLVTSAQ 118
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 157 FATTVEKALPSINVP--NILVASMGDmlgfkghivnlvvrrvkkmvPAwnIPGHVRFKDALAQGRAKSFNPVPVQGCDIA 234
Cdd:PRK08008 119 FYPMYRQIQQEDATPlrHICLTRVAL--------------------PA--DDGVSSFTQLKAQQPATLCYAPPLSTDDTA 176
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 235 FLQYTGGTTGISKGAMLTHSNIL-ANVeqMNLWmDVAFRNKGKpkalnFVCALPLYHI-FALTVnAMIGMKLGARNILip 312
Cdd:PRK08008 177 EILFTSGTTSRPKGVVITHYNLRfAGY--YSAW-QCALRDDDV-----YLTVMPAFHIdCQCTA-AMAAFSAGATFVL-- 245
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 313 nprdipsfvkeLKKYPVHIFPGlntlfnglmnnpdfQTLDFK-------PLIL-TLgggMaVQRPVAERWQH-----MTG 379
Cdd:PRK08008 246 -----------LEKYSARAFWG--------------QVCKYRatiteciPMMIrTL---M-VQPPSANDRQHclrevMFY 296
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 380 CHITE----------------GYGLSET-------SPVacanalDATEFTgTIGLPMPSTDVVIRDDDGSDLLLGKVGEI 436
Cdd:PRK08008 297 LNLSDqekdafeerfgvrlltSYGMTETivgiigdRPG------DKRRWP-SIGRPGFCYEAEIRDDHNRPLPAGEIGEI 369
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 437 CVRG---PQVMKGYWNRPEETARAIMADGFFRTGDMGFMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVAAEHPGVLES 513
Cdd:PRK08008 370 CIKGvpgKTIFKEYYLDPKATAKVLEADGWLHTGDTGYVDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDI 449
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 479816261 514 AAVGIPNEHSGEVVKLYVVRRDPE-LTEDEVKAFCAERLTNYKRPREVEFRESLPKSNVGKILRRELR 580
Cdd:PRK08008 450 VVVGIKDSIRDEAIKAFVVLNEGEtLSEEEFFAFCEQNMAKFKVPSYLEIRKDLPRNCSGKIIKKNLK 517
|
|
| DltA |
cd05945 |
D-alanine:D-alanyl carrier protein ligase (DltA) and similar proteins; This family includes ... |
61-579 |
1.61e-56 |
|
D-alanine:D-alanyl carrier protein ligase (DltA) and similar proteins; This family includes D-alanyl carrier protein ligase DltA and aliphatic beta-amino acid adenylation enzymes IdnL1 and CmiS6. DltA incorporates D-ala in techoic acids in gram-positive bacteria via a two-step process, starting with adenylation of D-alanine that transfers D-alanine to the D-alanyl carrier protein. IdnL1, a short-chain aliphatic beta-amino acid adenylation enzyme, recognizes 3-aminobutanoic acid, and is involved in the synthesis of the macrolactam antibiotic incednine. CmiS6 is a medium-chain beta-amino acid adenylation enzyme that recognizes 3-aminononanoic acid, and is involved in the synthesis of cremimycin, also a macrolactam antibiotic. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341267 [Multi-domain] Cd Length: 449 Bit Score: 196.70 E-value: 1.61e-56
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 61 RQFANSPAFTCMGKDISYKELDDHSRALAAWLQSRGLVKGDRVAIMMPNILQYPITFTAVLRAGFVVVNVNPLYTPRELE 140
Cdd:cd05945 2 AANPDRPAVVEGGRTLTYRELKERADALAAALASLGLDAGDPVVVYGHKSPDAIAAFLAALKAGHAYVPLDASSPAERIR 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 141 HQLNDAGAKALVVLENfattvekalpsinvpnilvasmgdmlgfkghivnlvvrrvkkmvpawnipghvrfkdalaqgra 220
Cdd:cd05945 82 EILDAAKPALLIADGD---------------------------------------------------------------- 97
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 221 ksfnpvpvqgcDIAFLQYTGGTTGISKGAMLTHSNILANVEqmnlWMDVAFrnkGKPKALNFVCALPlYHiFALTVNAmI 300
Cdd:cd05945 98 -----------DNAYIIFTSGSTGRPKGVQISHDNLVSFTN----WMLSDF---PLGPGDVFLNQAP-FS-FDLSVMD-L 156
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 301 GMKLGARNILIPNPRDIPSFVKEL----KKYPVHIFPGLNTLFNGLMNNPDFQTLDFKPLILTLGGGMAVQRPVAERWQ- 375
Cdd:cd05945 157 YPALASGATLVPVPRDATADPKQLfrflAEHGITVWVSTPSFAAMCLLSPTFTPESLPSLRHFLFCGEVLPHKTARALQq 236
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 376 HMTGCHITEGYGLSETSpVAC-ANALDATEFTG----TIGLPMPSTDVVIRDDDGSDLLLGKVGEICVRGPQVMKGYWNR 450
Cdd:cd05945 237 RFPDARIYNTYGPTEAT-VAVtYIEVTPEVLDGydrlPIGYAKPGAKLVILDEDGRPVPPGEKGELVISGPSVSKGYLNN 315
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 451 PEETARAI-MADGF--FRTGDMGFMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVAAEHPGVLESAAVGIPNEHSGEVV 527
Cdd:cd05945 316 PEKTAAAFfPDEGQraYRTGDLVRLEADGLLFYRGRLDFQVKLNGYRIELEEIEAALRQVPGVKEAVVVPKYKGEKVTEL 395
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....
gi 479816261 528 KLYVVRRDPELTED--EVKAFCAERLTNYKRPREVEFRESLPKSNVGKILRREL 579
Cdd:cd05945 396 IAFVVPKPGAEAGLtkAIKAELAERLPPYMIPRRFVYLDELPLNANGKIDRKAL 449
|
|
| PRK08162 |
PRK08162 |
acyl-CoA synthetase; Validated |
76-581 |
9.38e-56 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236169 [Multi-domain] Cd Length: 545 Bit Score: 197.09 E-value: 9.38e-56
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 76 ISYKELDDHSRALAAWLQSRGLVKGDRVAIMMPNIlqyPITFTA---VLRAGFVVVNVNPLYTPRELEHQLNDAGAKALV 152
Cdd:PRK08162 44 RTWAETYARCRRLASALARRGIGRGDTVAVLLPNI---PAMVEAhfgVPMAGAVLNTLNTRLDAASIAFMLRHGEAKVLI 120
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 153 VLENFATTVEKALPSINVPNILVASMGDMLGFKGHIVnlvvrrvkkmvpawnipGHVRFKDALAQGRAKSFNPVPVQGCD 232
Cdd:PRK08162 121 VDTEFAEVAREALALLPGPKPLVIDVDDPEYPGGRFI-----------------GALDYEAFLASGDPDFAWTLPADEWD 183
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 233 IAFLQYTGGTTGISKGAMLTH--------SNILAnveqmnlWmdvafrnkGKPKALNFVCALPLYHI----FALTVNAMI 300
Cdd:PRK08162 184 AIALNYTSGTTGNPKGVVYHHrgaylnalSNILA-------W--------GMPKHPVYLWTLPMFHCngwcFPWTVAARA 248
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 301 GMKLGARNIlipNPRDIPSFVKELKkypVHIFPGLNTLFNGLMNNPDFQTLDFKPLILTLGGGMAVQRPVAERWQHMtGC 380
Cdd:PRK08162 249 GTNVCLRKV---DPKLIFDLIREHG---VTHYCGAPIVLSALINAPAEWRAGIDHPVHAMVAGAAPPAAVIAKMEEI-GF 321
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 381 HITEGYGLSET-SPVA-CA-----NALDATE---FTGTIGLPMPSTD-VVIRD--------DDGSDLllgkvGEICVRGP 441
Cdd:PRK08162 322 DLTHVYGLTETyGPATvCAwqpewDALPLDEraqLKARQGVRYPLQEgVTVLDpdtmqpvpADGETI-----GEIMFRGN 396
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 442 QVMKGYWNRPEETARAiMADGFFRTGDMGFMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVAAEHPGVLESAAVGIPNE 521
Cdd:PRK08162 397 IVMKGYLKNPKATEEA-FAGGWFHTGDLAVLHPDGYIKIKDRSKDIIISGGENISSIEVEDVLYRHPAVLVAAVVAKPDP 475
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|.
gi 479816261 522 HSGEVVKLYV-VRRDPELTEDEVKAFCAERLTNYKRPREVEFREsLPKSNVGKILRRELRD 581
Cdd:PRK08162 476 KWGEVPCAFVeLKDGASATEEEIIAHCREHLAGFKVPKAVVFGE-LPKTSTGKIQKFVLRE 535
|
|
| MACS_AAE_MA_like |
cd05970 |
Medium-chain acyl-CoA synthetase (MACS) of AAE_MA like; MACS catalyzes the two-step activation ... |
74-581 |
1.01e-55 |
|
Medium-chain acyl-CoA synthetase (MACS) of AAE_MA like; MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This family of MACS enzymes is found in archaea and bacteria. It is represented by the acyl-adenylating enzyme from Methanosarcina acetivorans (AAE_MA). AAE_MA is most active with propionate, butyrate, and the branched analogs: 2-methyl-propionate, butyrate, and pentanoate. The specific activity is weaker for smaller or larger acids.
Pssm-ID: 341274 [Multi-domain] Cd Length: 537 Bit Score: 196.56 E-value: 1.01e-55
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 74 KDISYKELDDHSRALAAWLQSRGLVKGDRVAIMMPNILQYPITFTAVLRAGFVVVNVNPLYTPRELEHQLNDAGAKALVV 153
Cdd:cd05970 46 RIFTFAELADYSDKTANFFKAMGIGKGDTVMLTLKRRYEFWYSLLALHKLGAIAIPATHQLTAKDIVYRIESADIKMIVA 125
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 154 L--ENFATTVEKALPSINVPNILVASMGDML-GFkghiVNLVvRRVKKMVPAWNIPghvrfkdalaQGraksfnPVPVQG 230
Cdd:cd05970 126 IaeDNIPEEIEKAAPECPSKPKLVWVGDPVPeGW----IDFR-KLIKNASPDFERP----------TA------NSYPCG 184
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 231 CDIAFLQYTGGTTGISKgaMLTHSNI--LANVEQMNLWMDVafrnkgKPKALNFVCALPLYhifaltVNAMIGmKL---- 304
Cdd:cd05970 185 EDILLVYFSSGTTGMPK--MVEHDFTypLGHIVTAKYWQNV------REGGLHLTVADTGW------GKAVWG-KIygqw 249
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 305 --GARNILIPNPRDIP-SFVKELKKYPVHIFPGLNTLFNGLMNNpDFQTLDFKPLILTLGGGMAVQRPVAERWQHMTGCH 381
Cdd:cd05970 250 iaGAAVFVYDYDKFDPkALLEKLSKYGVTTFCAPPTIYRFLIRE-DLSRYDLSSLRYCTTAGEALNPEVFNTFKEKTGIK 328
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 382 ITEGYGLSETspVACANALDATEFT-GTIGLPMPSTDVVIRDDDGSDLLLGKVGEICVRGPQ-----VMKGYWNRPEETA 455
Cdd:cd05970 329 LMEGFGQTET--TLTIATFPWMEPKpGSMGKPAPGYEIDLIDREGRSCEAGEEGEIVIRTSKgkpvgLFGGYYKDAEKTA 406
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 456 RAiMADGFFRTGDMGFMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVAAEHPGVLESAAVGIPNEHSGEVVKLYVV-RR 534
Cdd:cd05970 407 EV-WHDGYYHTGDAAWMDEDGYLWFVGRTDDLIKSSGYRIGPFEVESALIQHPAVLECAVTGVPDPIRGQVVKATIVlAK 485
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|
gi 479816261 535 DPELTED---EVKAFCAERLTNYKRPREVEFRESLPKSNVGKILRRELRD 581
Cdd:cd05970 486 GYEPSEElkkELQDHVKKVTAPYKYPRIVEFVDELPKTISGKIRRVEIRE 535
|
|
| entE |
PRK10946 |
(2,3-dihydroxybenzoyl)adenylate synthase; |
52-580 |
6.52e-55 |
|
(2,3-dihydroxybenzoyl)adenylate synthase;
Pssm-ID: 236803 [Multi-domain] Cd Length: 536 Bit Score: 194.44 E-value: 6.52e-55
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 52 ISDMISASCRqfANSPAFTCMGKDISYKELDDHSRALAAWLQSRGLVKGDRVAIMMPNILQYPITFTAVLRAGfvVVNVN 131
Cdd:PRK10946 27 LTDILTRHAA--SDAIAVICGERQFSYRELNQASDNLACSLRRQGIKPGDTALVQLGNVAEFYITFFALLKLG--VAPVN 102
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 132 PLYTPRELEhqlndagakalvvLENFATTVEkalpsinvPNILVASMGDMLgFKGhivNLVVRRVKKMVPAWNI------ 205
Cdd:PRK10946 103 ALFSHQRSE-------------LNAYASQIE--------PALLIADRQHAL-FSD---DDFLNTLVAEHSSLRVvlllnd 157
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 206 PGHVRFKDALAQGrAKSFNPVPVQGCDIAFLQYTGGTTGISKGAMLTHSN----ILANVEQMNLwmDVAFRnkgkpkaln 281
Cdd:PRK10946 158 DGEHSLDDAINHP-AEDFTATPSPADEVAFFQLSGGSTGTPKLIPRTHNDyyysVRRSVEICGF--TPQTR--------- 225
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 282 FVCALPLYHIFALTVNAMIGMKL-GARNILIPNPRDIPSFvKELKKYPVHI---FPGLNTLFNGLMNNPDFQTlDFKPLI 357
Cdd:PRK10946 226 YLCALPAAHNYPMSSPGALGVFLaGGTVVLAPDPSATLCF-PLIEKHQVNVtalVPPAVSLWLQAIAEGGSRA-QLASLK 303
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 358 LTLGGGMAVQRPVAERWQHMTGCHITEGYGLSETspVACANALDATE---FTgTIGLPM-PSTDVVIRDDDGSDLLLGKV 433
Cdd:PRK10946 304 LLQVGGARLSETLARRIPAELGCQLQQVFGMAEG--LVNYTRLDDSDeriFT-TQGRPMsPDDEVWVADADGNPLPQGEV 380
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 434 GEICVRGPQVMKGYWNRPEETARAIMADGFFRTGDMGFMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVAAEHPGVLES 513
Cdd:PRK10946 381 GRLMTRGPYTFRGYYKSPQHNASAFDANGFYCSGDLVSIDPDGYITVVGREKDQINRGGEKIAAEEIENLLLRHPAVIHA 460
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 479816261 514 AAVGIPNEHSGEVVKLYVVRRDPeLTEDEVKAFCAER-LTNYKRPREVEFRESLPKSNVGKILRRELR 580
Cdd:PRK10946 461 ALVSMEDELMGEKSCAFLVVKEP-LKAVQLRRFLREQgIAEFKLPDRVECVDSLPLTAVGKVDKKQLR 527
|
|
| PRK07798 |
PRK07798 |
acyl-CoA synthetase; Validated |
51-573 |
7.40e-55 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236100 [Multi-domain] Cd Length: 533 Bit Score: 194.33 E-value: 7.40e-55
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 51 SISDMISASCRQFANSPAFTCMGKDISYKELDDHSRALAAWLQSRGLVKGDRVAIMMPNILQYPITFTAVLRAGFVVVNV 130
Cdd:PRK07798 4 NIADLFEAVADAVPDRVALVCGDRRLTYAELEERANRLAHYLIAQGLGPGDHVGIYARNRIEYVEAMLGAFKARAVPVNV 83
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 131 NPLYTPRELEHQLNDAGAKALVVLENFATTVEKALPSInvPNI-LVASMGDMLGfkghivnlvvrrvkkmvpAWNIPGHV 209
Cdd:PRK07798 84 NYRYVEDELRYLLDDSDAVALVYEREFAPRVAEVLPRL--PKLrTLVVVEDGSG------------------NDLLPGAV 143
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 210 RFKDALAQGRAksfNPVPVQGC--DIAFLqYTGGTTGISKGAMLTHSNI---------LANVEQMNLWMDVAFRNKGKPK 278
Cdd:PRK07798 144 DYEDALAAGSP---ERDFGERSpdDLYLL-YTGGTTGMPKGVMWRQEDIfrvllggrdFATGEPIEDEEELAKRAAAGPG 219
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 279 ALNFVCAlPLYHIFALTvNAMIGMKLGARNILIPNPR-DIPSFVKELKKYPVHIFpglnTLFNGLMNNPDFQTL------ 351
Cdd:PRK07798 220 MRRFPAP-PLMHGAGQW-AAFAALFSGQTVVLLPDVRfDADEVWRTIEREKVNVI----TIVGDAMARPLLDALeargpy 293
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 352 DFKPLILTLGGGMAVQRPVAERWQ-HMTGCHITEGYGLSET----SPVACANALDATEFTGTIGlpmPSTDVVirDDDGS 426
Cdd:PRK07798 294 DLSSLFAIASGGALFSPSVKEALLeLLPNVVLTDSIGSSETgfggSGTVAKGAVHTGGPRFTIG---PRTVVL--DEDGN 368
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 427 DLLLG--KVGEICVRGPqVMKGYWNRPEETARAimadgfFRT---------GDMGFMDERGYTKIVDRKKDMILVSGFNV 495
Cdd:PRK07798 369 PVEPGsgEIGWIARRGH-IPLGYYKDPEKTAET------FPTidgvryaipGDRARVEADGTITLLGRGSVCINTGGEKV 441
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 479816261 496 YPNEIEEVAAEHPGVLESAAVGIPNEHSG-EVVKLYVVRRDPELTEDEVKAFCAERLTNYKRPREVEFRESLPKSNVGK 573
Cdd:PRK07798 442 FPEEVEEALKAHPDVADALVVGVPDERWGqEVVAVVQLREGARPDLAELRAHCRSSLAGYKVPRAIWFVDEVQRSPAGK 520
|
|
| menE |
TIGR01923 |
O-succinylbenzoate-CoA ligase; This model represents an enzyme, O-succinylbenzoate-CoA ligase, ... |
77-579 |
1.29e-54 |
|
O-succinylbenzoate-CoA ligase; This model represents an enzyme, O-succinylbenzoate-CoA ligase, which is involved in the fourth step of the menaquinone biosynthesis pathway. O-succinylbenzoate-CoA ligase, together with menB - naphtoate synthase, take 2-succinylbenzoate and convert it into 1,4-di-hydroxy-2- naphtoate. [Biosynthesis of cofactors, prosthetic groups, and carriers, Menaquinone and ubiquinone]
Pssm-ID: 162605 [Multi-domain] Cd Length: 436 Bit Score: 191.12 E-value: 1.29e-54
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 77 SYKELDDHSRALAAWLQSRGLVKGDRVAIMMPNILQYPITFTAVLRAGFVVVNVNPLYTPRELEHQLNDAGAKALVVLEN 156
Cdd:TIGR01923 1 TWQDLDCEAAHLAKALKAQGIRSGSRVALVGQNSIEMVLLLHACLLLGAEIAMLNTRLTENERTNQLEDLDVQLLLTDSL 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 157 FAttvEKALPSINVPNILVASMGDmlgfkghiVNLvvrrvkkmvpawnipghvrfkdalaqgrAKSFNpvpvqGCDIAFL 236
Cdd:TIGR01923 81 LE---EKDFQADSLDRIEAAGRYE--------TSL----------------------------SASFN-----MDQIATL 116
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 237 QYTGGTTGISKGAMLTHSNILANVeqmnlwMDVAfRNKGKPKALNFVCALPLYHIFALTvnamIGMK--LGARNILIPNP 314
Cdd:TIGR01923 117 MFTSGTTGKPKAVPHTFRNHYASA------VGSK-ENLGFTEDDNWLLSLPLYHISGLS----ILFRwlIEGATLRIVDK 185
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 315 RDipSFVKELKKYPVHIFPGLNTLFNGLMNnpdfQTLDFKPLILTLGGGMAVQRPVAERWQHMtGCHITEGYGLSETSPV 394
Cdd:TIGR01923 186 FN--QLLEMIANERVTHISLVPTQLNRLLD----EGGHNENLRKILLGGSAIPAPLIEEAQQY-GLPIYLSYGMTETCSQ 258
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 395 ACANALDATEFTGTIGLPMPSTDVVIRDDDgsdllLGKVGEICVRGPQVMKGYWNrPEETARAIMADGFFRTGDMGFMDE 474
Cdd:TIGR01923 259 VTTATPEMLHARPDVGRPLAGREIKIKVDN-----KEGHGEIMVKGANLMKGYLY-QGELTPAFEQQGWFNTGDIGELDG 332
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 475 RGYTKIVDRKKDMILVSGFNVYPNEIEEVAAEHPGVLESAAVGIPNEHSGEVVKLYVVRRDPElTEDEVKAFCAERLTNY 554
Cdd:TIGR01923 333 EGFLYVLGRRDDLIISGGENIYPEEIETVLYQHPGIQEAVVVPKPDAEWGQVPVAYIVSESDI-SQAKLIAYLTEKLAKY 411
|
490 500
....*....|....*....|....*
gi 479816261 555 KRPREVEFRESLPKSNVGKILRREL 579
Cdd:TIGR01923 412 KVPIAFEKLDELPYNASGKILRNQL 436
|
|
| AA-adenyl-dom |
TIGR01733 |
amino acid adenylation domain; This model represents a domain responsible for the specific ... |
77-516 |
2.16e-54 |
|
amino acid adenylation domain; This model represents a domain responsible for the specific recognition of amino acids and activation as adenylyl amino acids. The reaction catalyzed is aa + ATP -> aa-AMP + PPi. These domains are usually found as components of multi-domain non-ribosomal peptide synthetases and are usually called "A-domains" in that context. A-domains are almost invariably followed by "T-domains" (thiolation domains, pfam00550) to which the amino acid adenylate is transferred as a thiol-ester to a bound pantetheine cofactor with the release of AMP (these are also called peptide carrier proteins, or PCPs. When the A-domain does not represent the first module (corresponding to the first amino acid in the product molecule) it is usually preceded by a "C-domain" (condensation domain, pfam00668) which catalyzes the ligation of two amino acid thiol-esters from neighboring modules. This domain is a subset of the AMP-binding domain found in Pfam (pfam00501) which also hits substrate--CoA ligases and luciferases. Sequences scoring in between trusted and noise for this model may be ambiguous as to whether they activate amino acids or other molecules lacking an alpha amino group.
Pssm-ID: 273779 [Multi-domain] Cd Length: 409 Bit Score: 189.78 E-value: 2.16e-54
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 77 SYKELDDHSRALAAWLQSRGLVK-GDRVAIMMPNILQYPITFTAVLRAGFVVVNVNPLYTPRELEHQLNDAGAKALVVLE 155
Cdd:TIGR01733 1 TYRELDERANRLARHLRAAGGVGpGDRVAVLLERSAELVVAILAVLKAGAAYVPLDPAYPAERLAFILEDAGARLLLTDS 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 156 NFATTVEKALPSINVPNILVASMGDmlgfkghivnlvvrrvkkmvpawnipghvrfkDALAQGRAksfnPVPVQGCDIAF 235
Cdd:TIGR01733 81 ALASRLAGLVLPVILLDPLELAALD--------------------------------DAPAPPPP----DAPSGPDDLAY 124
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 236 LQYTGGTTGISKGAMLTHSNILANVEqmnlWMDVAFRNKGKPKALNF---VCALPLYHIF-ALTVnamigmklGARNILI 311
Cdd:TIGR01733 125 VIYTSGSTGRPKGVVVTHRSLVNLLA----WLARRYGLDPDDRVLQFaslSFDASVEEIFgALLA--------GATLVVP 192
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 312 PNP--RDIPSFVKEL-KKYPVHIFPGLNTLFNGLMNNPDFQTLDFKPLILtlgGGMAVQRPVAERWQ-HMTGCHITEGYG 387
Cdd:TIGR01733 193 PEDeeRDDAALLAALiAEHPVTVLNLTPSLLALLAAALPPALASLRLVIL---GGEALTPALVDRWRaRGPGARLINLYG 269
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 388 LSETSPVACANALDATEFTGT----IGLPMPSTDVVIRDDDGSDLLLGKVGEICVRGPQVMKGYWNRPEETARAIMADGF 463
Cdd:TIGR01733 270 PTETTVWSTATLVDPDDAPREspvpIGRPLANTRLYVLDDDLRPVPVGVVGELYIGGPGVARGYLNRPELTAERFVPDPF 349
|
410 420 430 440 450 460
....*....|....*....|....*....|....*....|....*....|....*....|.
gi 479816261 464 --------FRTGDMGFMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVAAEHPGVlESAAV 516
Cdd:TIGR01733 350 aggdgarlYRTGDLVRYLPDGNLEFLGRIDDQVKIRGYRIELGEIEAALLRHPGV-REAVV 409
|
|
| AAS_C |
cd05909 |
C-terminal domain of the acyl-acyl carrier protein synthetase (also called ... |
73-581 |
3.68e-54 |
|
C-terminal domain of the acyl-acyl carrier protein synthetase (also called 2-acylglycerophosphoethanolamine acyltransferase, Aas); Acyl-acyl carrier protein synthase (Aas) is a membrane protein responsible for a minor pathway of incorporating exogenous fatty acids into membrane phospholipids. Its in vitro activity is characterized by the ligation of free fatty acids between 8 and 18 carbons in length to the acyl carrier protein sulfydryl group (ACP-SH) in the presence of ATP and Mg2+. However, its in vivo function is as a 2-acylglycerophosphoethanolamine (2-acyl-GPE) acyltransferase. The reaction occurs in two steps: the acyl chain is first esterified to acyl carrier protein (ACP) via a thioester bond, followed by a second step where the acyl chain is transferred to a 2-acyllysophospholipid, thus completing the transacylation reaction. This model represents the C-terminal domain of the enzyme, which belongs to the class I adenylate-forming enzyme family, including acyl-CoA synthetases.
Pssm-ID: 341235 [Multi-domain] Cd Length: 490 Bit Score: 191.39 E-value: 3.68e-54
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 73 GKDISYKELDDHSRALAAWLQsRGLVKGDRVAIMMPNILQYPITFTAVLRAGFVVVNVNPLYTPRELEHQLNDAGAKALV 152
Cdd:cd05909 5 GTSLTYRKLLTGAIALARKLA-KMTKEGENVGVMLPPSAGGALANFALALSGKVPVMLNYTAGLRELRACIKLAGIKTVL 83
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 153 VLENFattVEKAlpsiNVPNILVASMGdmlgfkghivnlvvrrvKKMVPAWNIPGHVRFKDALAQGRAKSFNP------- 225
Cdd:cd05909 84 TSKQF---IEKL----KLHHLFDVEYD-----------------ARIVYLEDLRAKISKADKCKAFLAGKFPPkwllrif 139
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 226 --VPVQGCDIAFLQYTGGTTGISKGAMLTHSNILANVEQMNLWMDvafrnkgkPKALNFVC-ALPLYHIFALTVNAMIGM 302
Cdd:cd05909 140 gvAPVQPDDPAVILFTSGSEGLPKGVVLSHKNLLANVEQITAIFD--------PNPEDVVFgALPFFHSFGLTGCLWLPL 211
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 303 KLGARNILIPNP---RDIPSFVKELKkypVHIFPGLNTLFNGLMN--NPDfqtlDFKPLILTLGGGMAVQRPVAERWQHM 377
Cdd:cd05909 212 LSGIKVVFHPNPldyKKIPELIYDKK---ATILLGTPTFLRGYARaaHPE----DFSSLRLVVAGAEKLKDTLRQEFQEK 284
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 378 TGCHITEGYGLSETSPVACANALDATEFTGTIGLPMPSTDVVIRD-DDGSDLLLGKVGEICVRGPQVMKGYWNRPEETAr 456
Cdd:cd05909 285 FGIRILEGYGTTECSPVISVNTPQSPNKEGTVGRPLPGMEVKIVSvETHEEVPIGEGGLLLVRGPNVMLGYLNEPELTS- 363
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 457 AIMADGFFRTGDMGFMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVAAEH-PGVLESAAVGIPNEHSGEVVKLYVVrrD 535
Cdd:cd05909 364 FAFGDGWYDTGDIGKIDGEGFLTITGRLSRFAKIAGEMVSLEAIEDILSEIlPEDNEVAVVSVPDGRKGEKIVLLTT--T 441
|
490 500 510 520
....*....|....*....|....*....|....*....|....*..
gi 479816261 536 PELTEDEVKAFCAE-RLTNYKRPREVEFRESLPKSNVGKILRRELRD 581
Cdd:cd05909 442 TDTDPSSLNDILKNaGISNLAKPSYIHQVEEIPLLGTGKPDYVTLKA 488
|
|
| A_NRPS_Srf_like |
cd12117 |
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Bacillus subtilis ... |
66-579 |
6.77e-54 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Bacillus subtilis termination module Surfactin (SrfA-C); The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and, in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the adenylation domain of the Bacillus subtilis termination module (Surfactin domain, SrfA-C) which recognizes a specific amino acid building block, which is then activated and transferred to the terminal thiol of the 4'-phosphopantetheine (Ppan) arm of the downstream peptidyl carrier protein (PCP) domain.
Pssm-ID: 341282 [Multi-domain] Cd Length: 483 Bit Score: 190.49 E-value: 6.77e-54
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 66 SPAFTCMGKDISYKELDDHSRALAAWLQSRGLVKGDRVAIMMPNILQYPITFTAVLRAGFVVVNVNPLYTPRELEHQLND 145
Cdd:cd12117 13 AVAVVYGDRSLTYAELNERANRLARRLRAAGVGPGDVVGVLAERSPELVVALLAVLKAGAAYVPLDPELPAERLAFMLAD 92
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 146 AGAKALVVLENFATTVEkalpsinvpnilvasmgdmlgfkGHIVNLVVRRVKKMVPAWNIPghvrfkdalaqgraksfnp 225
Cdd:cd12117 93 AGAKVLLTDRSLAGRAG-----------------------GLEVAVVIDEALDAGPAGNPA------------------- 130
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 226 VPVQGCDIAFLQYTGGTTGISKGAMLTHSNILANVEQMNlWMDVAFRNKgkpkalnFVCALPL------YHIFALTVNam 299
Cdd:cd12117 131 VPVSPDDLAYVMYTSGSTGRPKGVAVTHRGVVRLVKNTN-YVTLGPDDR-------VLQTSPLafdastFEIWGALLN-- 200
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 300 igmklGARNILIP--NPRDIPSFVKELKKYPVHIFPGLNTLFNGLMN-NPD-FQTLDFkplILTlgGGMAVQRPVAERW- 374
Cdd:cd12117 201 -----GARLVLAPkgTLLDPDALGALIAEEGVTVLWLTAALFNQLADeDPEcFAGLRE---LLT--GGEVVSPPHVRRVl 270
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 375 QHMTGCHITEGYGLSETSPVACANALDATEFTGT---IGLPMPSTDVVIRDDDGSDLLLGKVGEICVRGPQVMKGYWNRP 451
Cdd:cd12117 271 AACPGLRLVNGYGPTENTTFTTSHVVTELDEVAGsipIGRPIANTRVYVLDEDGRPVPPGVPGELYVGGDGLALGYLNRP 350
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 452 EETARAIMADGF------FRTGDMGFMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVAAEHPGVLESAAVGIPNEHSGE 525
Cdd:cd12117 351 ALTAERFVADPFgpgerlYRTGDLARWLPDGRLEFLGRIDDQVKIRGFRIELGEIEAALRAHPGVREAVVVVREDAGGDK 430
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....
gi 479816261 526 VVKLYVVrRDPELTEDEVKAFCAERLTNYKRPREVEFRESLPKSNVGKILRREL 579
Cdd:cd12117 431 RLVAYVV-AEGALDAAELRAFLRERLPAYMVPAAFVVLDELPLTANGKVDRRAL 483
|
|
| CBAL |
cd05923 |
4-Chlorobenzoate-CoA ligase (CBAL); CBAL catalyzes the conversion of 4-chlorobenzoate (4-CB) ... |
73-579 |
1.23e-53 |
|
4-Chlorobenzoate-CoA ligase (CBAL); CBAL catalyzes the conversion of 4-chlorobenzoate (4-CB) to 4-chlorobenzoyl-coenzyme A (4-CB-CoA) by the two-step adenylation and thioester-forming reactions. 4-Chlorobenzoate (4-CBA) is an environmental pollutant derived from microbial breakdown of aromatic pollutants, such as polychlorinated biphenyls (PCBs), DDT, and certain herbicides. The 4-CBA degrading pathway converts 4-CBA to the metabolite 4-hydroxybezoate (4-HBA), allowing some soil-dwelling microbes to utilize 4-CBA as an alternate carbon source. This pathway consists of three chemical steps catalyzed by 4-CBA-CoA ligase, 4-CBA-CoA dehalogenase, and 4HBA-CoA thioesterase in sequential reactions.
Pssm-ID: 341247 [Multi-domain] Cd Length: 493 Bit Score: 190.03 E-value: 1.23e-53
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 73 GKDISYKELDDHSRALAAWLQSRGLVKGDRVAIMMPNILQYPITFTAVLRAGFVVVNVNPLYTPRELEhQLNDAGAKALV 152
Cdd:cd05923 26 GLRLTYSELRARIEAVAARLHARGLRPGQRVAVVLPNSVEAVIALLALHRLGAVPALINPRLKAAELA-ELIERGEMTAA 104
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 153 VLENFATTVekalPSINVPNILVASMGDMLGfkghivnlvvrrvkkmvpawnipghvrfkDALAQGRAKSFNPVPVQGCD 232
Cdd:cd05923 105 VIAVDAQVM----DAIFQSGVRVLALSDLVG-----------------------------LGEPESAGPLIEDPPREPEQ 151
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 233 IAFLQYTGGTTGISKGAMLTH----SNILANVEQMNLwmdvafrnkgKPKALNFVCAL-PLYHI---FALTVNAMIGmkl 304
Cdd:cd05923 152 PAFVFYTSGTTGLPKGAVIPQraaeSRVLFMSTQAGL----------RHGRHNVVLGLmPLYHVigfFAVLVAALAL--- 218
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 305 GARNILIP--NPRDIPSFVKELKKYPVHIFPglnTLFNGLMNNPDFQTLDFKPLILTLGGGMAVQRPVAERW-QHMTGcH 381
Cdd:cd05923 219 DGTYVVVEefDPADALKLIEQERVTSLFATP---THLDALAAAAEFAGLKLSSLRHVTFAGATMPDAVLERVnQHLPG-E 294
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 382 ITEGYGLSETspvacANAL---DATEftGTIGLPMPSTDV-VIRDDDGSDLLL--GKVGEICVR--GPQVMKGYWNRPEE 453
Cdd:cd05923 295 KVNIYGTTEA-----MNSLymrDART--GTEMRPGFFSEVrIVRIGGSPDEALanGEEGELIVAaaADAAFTGYLNQPEA 367
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 454 TARAImADGFFRTGDMGFMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVAAEHPGVLESAAVGIPNEHSGEVVKLYVVR 533
Cdd:cd05923 368 TAKKL-QDGWYRTGDVGYVDPSGDVRILGRVDDMIISGGENIHPSEIERVLSRHPGVTEVVVIGVADERWGQSVTACVVP 446
|
490 500 510 520
....*....|....*....|....*....|....*....|....*..
gi 479816261 534 RDPELTEDEVKAFC-AERLTNYKRPREVEFRESLPKSNVGKILRREL 579
Cdd:cd05923 447 REGTLSADELDQFCrASELADFKRPRRYFFLDELPKNAMNKVLRRQL 493
|
|
| PRK06155 |
PRK06155 |
crotonobetaine/carnitine-CoA ligase; Provisional |
51-581 |
1.45e-51 |
|
crotonobetaine/carnitine-CoA ligase; Provisional
Pssm-ID: 235719 [Multi-domain] Cd Length: 542 Bit Score: 185.35 E-value: 1.45e-51
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 51 SISDMISASCRQFANSPAFTCMGKDISYKELDDHSRALAAWLQSRGLVKGDRVAIMMPNILQYPITFTAVLRAGFVVVNV 130
Cdd:PRK06155 22 TLPAMLARQAERYPDRPLLVFGGTRWTYAEAARAAAAAAHALAAAGVKRGDRVALMCGNRIEFLDVFLGCAWLGAIAVPI 101
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 131 NPLYTPRELEHQLNDAGAKALVVLENFATTVEKALPSinvpnilVASMGDMLGFKGhivnlvvrrvkkmVPAWNIPGHVR 210
Cdd:PRK06155 102 NTALRGPQLEHILRNSGARLLVVEAALLAALEAADPG-------DLPLPAVWLLDA-------------PASVSVPAGWS 161
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 211 FKD--ALAQGRAksfnPVPVQGCDIAFLQYTGGTTGISKGAMLTHSnilanveQMNLW--MDVAFRNKGKPKALnFVCaL 286
Cdd:PRK06155 162 TAPlpPLDAPAP----AAAVQPGDTAAILYTSGTTGPSKGVCCPHA-------QFYWWgrNSAEDLEIGADDVL-YTT-L 228
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 287 PLYHIFALtvNAMI-GMKLGARNILipNPRDIPS-FVKELKKYPVHIFPGLNTLFNGLMNNPDFQTLDFKPLILTLGGGM 364
Cdd:PRK06155 229 PLFHTNAL--NAFFqALLAGATYVL--EPRFSASgFWPAVRRHGATVTYLLGAMVSILLSQPARESDRAHRVRVALGPGV 304
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 365 --AVQRPVAERwqhmTGCHITEGYGLSETSpVACANALDATEfTGTIGLPMPSTDVVIRDDDGSDLLLGKVGEICVRGPQ 442
Cdd:PRK06155 305 paALHAAFRER----FGVDLLDGYGSTETN-FVIAVTHGSQR-PGSMGRLAPGFEARVVDEHDQELPDGEPGELLLRADE 378
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 443 ---VMKGYWNRPEETARAiMADGFFRTGDMGFMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVAAEHPGVLESAAVGIP 519
Cdd:PRK06155 379 pfaFATGYFGMPEKTVEA-WRNLWFHTGDRVVRDADGWFRFVDRIKDAIRRRGENISSFEVEQVLLSHPAVAAAAVFPVP 457
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|...
gi 479816261 520 NEHSGEVVKLYVVRRDPE-LTEDEVKAFCAERLTNYKRPREVEFRESLPKSNVGKILRRELRD 581
Cdd:PRK06155 458 SELGEDEVMAAVVLRDGTaLEPVALVRHCEPRLAYFAVPRYVEFVAALPKTENGKVQKFVLRE 520
|
|
| MACS_like_4 |
cd05969 |
Uncharacterized subfamily of Acetyl-CoA synthetase like family (ACS); This family is most ... |
77-580 |
2.97e-50 |
|
Uncharacterized subfamily of Acetyl-CoA synthetase like family (ACS); This family is most similar to acetyl-CoA synthetase. Acetyl-CoA synthetase (ACS) catalyzes the formation of acetyl-CoA from acetate, CoA, and ATP. Synthesis of acetyl-CoA is carried out in a two-step reaction. In the first step, the enzyme catalyzes the synthesis of acetyl-AMP intermediate from acetate and ATP. In the second step, acetyl-AMP reacts with CoA to produce acetyl-CoA. This enzyme is only present in bacteria.
Pssm-ID: 341273 [Multi-domain] Cd Length: 442 Bit Score: 179.62 E-value: 2.97e-50
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 77 SYKELDDHSRALAAWLQSRGLVKGDRVAIMMPNILQYPITFTAVLRAGFVVVnvnPLYT---PRELEHQLNDAGAKALVV 153
Cdd:cd05969 2 TFAQLKVLSARFANVLKSLGVGKGDRVFVLSPRSPELYFSMLGIGKIGAVIC---PLFSafgPEAIRDRLENSEAKVLIT 78
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 154 LENFAttvekalpsinvpnilvasmgdmlgfkghivnlvvrrvKKMVPAwnipghvrfkdalaqgraksfnpvpvqgcDI 233
Cdd:cd05969 79 TEELY--------------------------------------ERTDPE-----------------------------DP 91
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 234 AFLQYTGGTTGISKGAMLTHSNILANveqmnlWMDVAFRNKGKPKALNFVCALPLYhiFALTVNAMIGMKL-GARNILIP 312
Cdd:cd05969 92 TLLHYTSGTTGTPKGVLHVHDAMIFY------YFTGKYVLDLHPDDIYWCTADPGW--VTGTVYGIWAPWLnGVTNVVYE 163
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 313 NPRDIPSFVKELKKYPVHIFPGLNTLFNGLMNNPDF--QTLDFKPLILTLGGGMAVQrPVAERWQH-MTGCHITEGYGLS 389
Cdd:cd05969 164 GRFDAESWYGIIERVKVTVWYTAPTAIRMLMKEGDElaRKYDLSSLRFIHSVGEPLN-PEAIRWGMeVFGVPIHDTWWQT 242
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 390 ETSPVACANALDATEFTGTIGLPMPSTDVVIRDDDGSDLLLGKVGEICVRG--PQVMKGYWNRPEETARAIMaDGFFRTG 467
Cdd:cd05969 243 ETGSIMIANYPCMPIKPGSMGKPLPGVKAAVVDENGNELPPGTKGILALKPgwPSMFRGIWNDEERYKNSFI-DGWYLTG 321
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 468 DMGFMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVAAEHPGVLESAAVGIPNEHSGEVVKLYVV-RRDPELTE---DEV 543
Cdd:cd05969 322 DLAYRDEDGYFWFVGRADDIIKTSGHRVGPFEVESALMEHPAVAEAGVIGKPDPLRGEIIKAFISlKEGFEPSDelkEEI 401
|
490 500 510
....*....|....*....|....*....|....*..
gi 479816261 544 KAFCAERLTNYKRPREVEFRESLPKSNVGKILRRELR 580
Cdd:cd05969 402 INFVRQKLGAHVAPREIEFVDNLPKTRSGKIMRRVLK 438
|
|
| EntF |
COG1020 |
EntF, seryl-AMP synthase component of non-ribosomal peptide synthetase [Secondary metabolites ... |
48-577 |
7.97e-50 |
|
EntF, seryl-AMP synthase component of non-ribosomal peptide synthetase [Secondary metabolites biosynthesis, transport and catabolism];
Pssm-ID: 440643 [Multi-domain] Cd Length: 1329 Bit Score: 186.22 E-value: 7.97e-50
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 48 AATSISDMISASCRQFANSPAFTCMGKDISYKELDDHSRALAAWLQSRGLVKGDRVAIMMPNILQYPITFTAVLRAGFVV 127
Cdd:COG1020 474 ADATLHELFEAQAARTPDAVAVVFGDQSLTYAELNARANRLAHHLRALGVGPGDLVGVCLERSLEMVVALLAVLKAGAAY 553
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 128 VNVNPLYtPRE-LEHQLNDAGAKALVVLENFATtvekALPSINVPNILVasmgdmlgfkghivnlvvrrvkkmvpawnip 206
Cdd:COG1020 554 VPLDPAY-PAErLAYMLEDAGARLVLTQSALAA----RLPELGVPVLAL------------------------------- 597
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 207 ghvrfkDALAQGRAKSFNP-VPVQGCDIAFLQYTGGTTGISKGAMLTHSNILANVEQMNLWMDVAfrnkgkPK------- 278
Cdd:COG1020 598 ------DALALAAEPATNPpVPVTPDDLAYVIYTSGSTGRPKGVMVEHRALVNLLAWMQRRYGLG------PGdrvlqfa 665
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 279 ALNFVCALplYHIF-ALTVnamigmklGARNILIPN--PRDIPSFVKELKKYPVHIF---PglnTLFNGLMNNPDFQTLD 352
Cdd:COG1020 666 SLSFDASV--WEIFgALLS--------GATLVLAPPeaRRDPAALAELLARHRVTVLnltP---SLLRALLDAAPEALPS 732
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 353 FKPLILtlgGGMAVQRPVAERW-QHMTGCHITEGYGLSETSPVACANALDATEFTG---TIGLPMPSTDVVIRDDDGSDL 428
Cdd:COG1020 733 LRLVLV---GGEALPPELVRRWrARLPGARLVNLYGPTETTVDSTYYEVTPPDADGgsvPIGRPIANTRVYVLDAHLQPV 809
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 429 LLGKVGEICVRGPQVMKGYWNRPEETARAIMADGF-------FRTGDMGFMDERG---YtkiVDRKKDMILVSGFNVYPN 498
Cdd:COG1020 810 PVGVPGELYIGGAGLARGYLNRPELTAERFVADPFgfpgarlYRTGDLARWLPDGnleF---LGRADDQVKIRGFRIELG 886
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 479816261 499 EIEEVAAEHPGVLESAAVGIPNEHSGEVVKLYVVRRDPELTEDEVKAFCAERLTNYKRPREVEFRESLPKSNVGKILRR 577
Cdd:COG1020 887 EIEAALLQHPGVREAVVVAREDAPGDKRLVAYVVPEAGAAAAAALLRLALALLLPPYMVPAAVVLLLPLPLTGNGKLDR 965
|
|
| A_NRPS_TubE_like |
cd05906 |
The adenylation domain (A domain) of a family of nonribosomal peptide synthetases (NRPSs) ... |
76-581 |
1.03e-49 |
|
The adenylation domain (A domain) of a family of nonribosomal peptide synthetases (NRPSs) synthesizing toxins and antitumor agents; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino)-acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. This family includes NRPSs that synthesize toxins and antitumor agents; for example, TubE for Tubulysine, CrpA for cryptophycin, TdiA for terrequinone A, KtzG for kutzneride, and Vlm1/Vlm2 for Valinomycin. Nonribosomal peptide synthetases are large multifunctional enzymes which synthesize many therapeutically useful peptides. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and, in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341232 [Multi-domain] Cd Length: 540 Bit Score: 180.17 E-value: 1.03e-49
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 76 ISYKELDDHSRALAAWLQSRGLVKGDRVAIMMPNILQYPITFTAVLRAGFVVVNVNPLYTPRELEHQLNDagakalvvLE 155
Cdd:cd05906 40 QSYQDLLEDARRLAAGLRQLGLRPGDSVILQFDDNEDFIPAFWACVLAGFVPAPLTVPPTYDEPNARLRK--------LR 111
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 156 NFATTVEKalPSINVPNILVASMGDMLGFKGHIVNLVVRRVKKMVPAWNIPGHvrfkdalaqgraksfnpvPVQGCDIAF 235
Cdd:cd05906 112 HIWQLLGS--PVVLTDAELVAEFAGLETLSGLPGIRVLSIEELLDTAADHDLP------------------QSRPDDLAL 171
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 236 LQYTGGTTGISKGAMLTHSNILA---NVEQMNL---------WMdvafrnkgkpkALNFVCALPLYHIFAltvnamigMK 303
Cdd:cd05906 172 LMLTSGSTGFPKAVPLTHRNILArsaGKIQHNGltpqdvflnWV-----------PLDHVGGLVELHLRA--------VY 232
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 304 LGARNILIPNP---RDIPSFVKELKKYPVHIFPGLNTLFnGLMNN----PDFQTLDFKPLILTLGGGMAVQRPVAERWQH 376
Cdd:cd05906 233 LGCQQVHVPTEeilADPLRWLDLIDRYRVTITWAPNFAF-ALLNDlleeIEDGTWDLSSLRYLVNAGEAVVAKTIRRLLR 311
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 377 MTGCH------ITEGYGLSETSPVACANALDAT-------EFTgTIGLPMPSTDVVIRDDDGSDLLLGKVGEICVRGPQV 443
Cdd:cd05906 312 LLEPYglppdaIRPAFGMTETCSGVIYSRSFPTydhsqalEFV-SLGRPIPGVSMRIVDDEGQLLPEGEVGRLQVRGPVV 390
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 444 MKGYWNRPEETARAIMADGFFRTGDMGFMDERGYTkIVDRKKDMILVSGFNVYPNEIEEVAAEHPGVLES--AAVGI--P 519
Cdd:cd05906 391 TKGYYNNPEANAEAFTEDGWFRTGDLGFLDNGNLT-ITGRTKDTIIVNGVNYYSHEIEAAVEEVPGVEPSftAAFAVrdP 469
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 479816261 520 NEHSGEVVKLYVVRRDPEL----TEDEVKAFCAERLT---NYKRPREvefRESLPKSNVGKILRRELRD 581
Cdd:cd05906 470 GAETEELAIFFVPEYDLQDalseTLRAIRSVVSREVGvspAYLIPLP---KEEIPKTSLGKIQRSKLKA 535
|
|
| LC_FACS_like |
cd17640 |
Long-chain fatty acid CoA synthetase; This family includes long-chain fatty acid (C12-C20) CoA ... |
74-508 |
3.48e-49 |
|
Long-chain fatty acid CoA synthetase; This family includes long-chain fatty acid (C12-C20) CoA synthetases, including an Arabidopsis gene At4g14070 that plays a role in activation and elongation of exogenous fatty acids. FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Eukaryotes generally have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.
Pssm-ID: 341295 [Multi-domain] Cd Length: 468 Bit Score: 177.17 E-value: 3.48e-49
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 74 KDISYKELDDHSRALAAWLQSRGLVKGDRVAIMMPNILQYPITFTAVLRAGFVVVNVNPLYTPRELEHQLNDAGAKALVV 153
Cdd:cd17640 4 KRITYKDLYQEILDFAAGLRSLGVKAGEKVALFADNSPRWLIADQGIMALGAVDVVRGSDSSVEELLYILNHSESVALVV 83
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 154 lENfattvekalpsinvpnilvasmgdmlgfkghivnlvvrrvkkmvpawnipghvrfkdalaqgraksfnpvpvQGCDI 233
Cdd:cd17640 84 -EN------------------------------------------------------------------------DSDDL 90
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 234 AFLQYTGGTTGISKGAMLTHSNILANVEQMNLWMDVafrNKGKPkalnFVCALPLYHIFALTVNAMIGMKLGARNIlipn 313
Cdd:cd17640 91 ATIIYTSGTTGNPKGVMLTHANLLHQIRSLSDIVPP---QPGDR----FLSILPIWHSYERSAEYFIFACGCSQAY---- 159
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 314 pRDIPSFVKELKKYPVHIFPGLNTLFNGLMNN--------PDFQTLDFKPLIL--------TLGGGMAvqrPVAERWQHM 377
Cdd:cd17640 160 -TSIRTLKDDLKRVKPHYIVSVPRLWESLYSGiqkqvsksSPIKQFLFLFFLSggifkfgiSGGGALP---PHVDTFFEA 235
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 378 TGCHITEGYGLSETSPVACANALDATeFTGTIGLPMPSTDVVIRDDDGSDLL-LGKVGEICVRGPQVMKGYWNRPEETAR 456
Cdd:cd17640 236 IGIEVLNGYGLTETSPVVSARRLKCN-VRGSVGRPLPGTEIKIVDPEGNVVLpPGEKGIVWVRGPQVMKGYYKNPEATSK 314
|
410 420 430 440 450
....*....|....*....|....*....|....*....|....*....|...
gi 479816261 457 AIMADGFFRTGDMGFMDERGYTKIVDRKKDMILVS-GFNVYPNEIEEVAAEHP 508
Cdd:cd17640 315 VLDSDGWFNTGDLGWLTCGGELVLTGRAKDTIVLSnGENVEPQPIEEALMRSP 367
|
|
| A_NRPS_AB3403-like |
cd17646 |
Peptide Synthetase; The adenylation (A) domain of NRPS recognizes a specific amino acid or ... |
54-579 |
6.97e-49 |
|
Peptide Synthetase; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341301 [Multi-domain] Cd Length: 488 Bit Score: 177.08 E-value: 6.97e-49
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 54 DMISASCRQFANSPAFTCMGKDISYKELDDHSRALAAWLQSRGLVKGDRVAIMMPNILQYPITFTAVLRAGFVVVNVNPL 133
Cdd:cd17646 2 ALVAEQAARTPDAPAVVDEGRTLTYRELDERANRLAHLLRARGVGPEDRVAVLLPRSADLVVALLAVLKAGAAYLPLDPG 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 134 YTPRELEHQLNDAGAKalVVLenfATTVEKALPSinvpnilvasmgdmlgfKGHIVNLVvrrvkkmvpawnipGHVRFKD 213
Cdd:cd17646 82 YPADRLAYMLADAGPA--VVL---TTADLAARLP-----------------AGGDVALL--------------GDEALAA 125
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 214 ALAQGRAksfnpVPVQGCDIAFLQYTGGTTGISKGAMLTHSNIlanVEQMnLWMDVAFRNKGKPKALnfvCALPLYhiFA 293
Cdd:cd17646 126 PPATPPL-----VPPRPDNLAYVIYTSGSTGRPKGVMVTHAGI---VNRL-LWMQDEYPLGPGDRVL---QKTPLS--FD 191
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 294 LTVNAMIG-MKLGARnILIPNP---RDIPSFVKELKKYPV---HIFPGLNTLFNGLMNNPDFQTLDfkpliLTLGGGMAV 366
Cdd:cd17646 192 VSVWELFWpLVAGAR-LVVARPgghRDPAYLAALIREHGVttcHFVPSMLRVFLAEPAAGSCASLR-----RVFCSGEAL 265
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 367 QRPVAERWQHMTGCHITEGYGLSETSPVACANALDATEFTGT--IGLPMPSTDVVIRDDDGSDLLLGKVGEICVRGPQVM 444
Cdd:cd17646 266 PPELAARFLALPGAELHNLYGPTEAAIDVTHWPVRGPAETPSvpIGRPVPNTRLYVLDDALRPVPVGVPGELYLGGVQLA 345
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 445 KGYWNRPEETARAIMADGF------FRTGDMGFMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVAAEHPGVLESAAVGI 518
Cdd:cd17646 346 RGYLGRPALTAERFVPDPFgpgsrmYRTGDLARWRPDGALEFLGRSDDQVKIRGFRVEPGEIEAALAAHPAVTHAVVVAR 425
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|...
gi 479816261 519 PNEHSGEVVKLYVVRRD--PELTEDEVKAFCAERLTNYKRPREVEFRESLPKSNVGKILRREL 579
Cdd:cd17646 426 AAPAGAARLVGYVVPAAgaAGPDTAALRAHLAERLPEYMVPAAFVVLDALPLTANGKLDRAAL 488
|
|
| PtmA |
cd17636 |
long-chain fatty acid CoA ligase (FadD); This family contains fatty acid CoA ligases, ... |
238-568 |
7.15e-49 |
|
long-chain fatty acid CoA ligase (FadD); This family contains fatty acid CoA ligases, including acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II, most of which are yet to be characterized. Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341291 [Multi-domain] Cd Length: 331 Bit Score: 172.87 E-value: 7.15e-49
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 238 YTGGTTGISKGAMLTHSNILAnveqMNLwmdVAFRNKGKPKALNFVCALPLYHI----FALTVNAMigmklGARNILIP- 312
Cdd:cd17636 7 YTAAFSGRPNGALLSHQALLA----QAL---VLAVLQAIDEGTVFLNSGPLFHIgtlmFTLATFHA-----GGTNVFVRr 74
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 313 -NPRDI---------------PSFVKELKKypvhifpgLNTLFNglmnnPDFQTLDFKPLILTLGGGMAVQRPvaeRWQH 376
Cdd:cd17636 75 vDAEEVlelieaercthafllPPTIDQIVE--------LNADGL-----YDLSSLRSSPAAPEWNDMATVDTS---PWGR 138
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 377 MTGchiteGYGLSETSPVACANALDATEfTGTIGLPMPSTDVVIRDDDGSDLLLGKVGEICVRGPQVMKGYWNRPEETAR 456
Cdd:cd17636 139 KPG-----GYGQTEVMGLATFAALGGGA-IGGAGRPSPLVQVRILDEDGREVPDGEVGEIVARGPTVMAGYWNRPEVNAR 212
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 457 AiMADGFFRTGDMGFMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVAAEHPGVLESAAVGIPNEHSGEVVKLYVVRRD- 535
Cdd:cd17636 213 R-TRGGWHHTNDLGRREPDGSLSFVGPKTRMIKSGAENIYPAEVERCLRQHPAVADAAVIGVPDPRWAQSVKAIVVLKPg 291
|
330 340 350
....*....|....*....|....*....|...
gi 479816261 536 PELTEDEVKAFCAERLTNYKRPREVEFRESLPK 568
Cdd:cd17636 292 ASVTEAELIEHCRARIASYKKPKSVEFADALPR 324
|
|
| BACL_like |
cd05929 |
Bacterial Bile acid CoA ligases and similar proteins; Bile acid-Coenzyme A ligase catalyzes ... |
236-581 |
1.62e-48 |
|
Bacterial Bile acid CoA ligases and similar proteins; Bile acid-Coenzyme A ligase catalyzes the formation of bile acid-CoA conjugates in a two-step reaction: the formation of a bile acid-AMP molecule as an intermediate, followed by the formation of a bile acid-CoA. This ligase requires a bile acid with a free carboxyl group, ATP, Mg2+, and CoA for synthesis of the final bile acid-CoA conjugate. The bile acid-CoA ligation is believed to be the initial step in the bile acid 7alpha-dehydroxylation pathway in the intestinal bacterium Eubacterium sp.
Pssm-ID: 341252 [Multi-domain] Cd Length: 473 Bit Score: 175.64 E-value: 1.62e-48
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 236 LQYTGGTTGISKGAMLTHSNILANVEQMNLWMDVAFRNKGKpkalNFVCALPLYHIFALTVNaMIGMKLGARNILIPNpR 315
Cdd:cd05929 130 MLYSGGTTGRPKGIKRGLPGGPPDNDTLMAAALGFGPGADS----VYLSPAPLYHAAPFRWS-MTALFMGGTLVLMEK-F 203
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 316 DIPSFVKELKKYPVHIFPGLNTLFNGLMNNP-------DFQTLdfKPLILTlGGGMAVqrPVAERWQHMTGCHITEGYGL 388
Cdd:cd05929 204 DPEEFLRLIERYRVTFAQFVPTMFVRLLKLPeavrnayDLSSL--KRVIHA-AAPCPP--WVKEQWIDWGGPIIWEYYGG 278
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 389 SETSPVACANALDATEFTGTIGLPMPStDVVIRDDDGSDLLLGKVGEICVRGPQVmKGYWNRPEETARAIMADGFFRTGD 468
Cdd:cd05929 279 TEGQGLTIINGEEWLTHPGSVGRAVLG-KVHILDEDGNEVPPGEIGEVYFANGPG-FEYTNDPEKTAAARNEGGWSTLGD 356
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 469 MGFMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVAAEHPGVLESAAVGIPNEHSGEVVKLYV-----VRRDPELtEDEV 543
Cdd:cd05929 357 VGYLDEDGYLYLTDRRSDMIISGGVNIYPQEIENALIAHPKVLDAAVVGVPDEELGQRVHAVVqpapgADAGTAL-AEEL 435
|
330 340 350
....*....|....*....|....*....|....*...
gi 479816261 544 KAFCAERLTNYKRPREVEFRESLPKSNVGKILRRELRD 581
Cdd:cd05929 436 IAFLRDRLSRYKCPRSIEFVAELPRDDTGKLYRRLLRD 473
|
|
| PRK08633 |
PRK08633 |
2-acyl-glycerophospho-ethanolamine acyltransferase; Validated |
72-574 |
7.97e-48 |
|
2-acyl-glycerophospho-ethanolamine acyltransferase; Validated
Pssm-ID: 236315 [Multi-domain] Cd Length: 1146 Bit Score: 180.12 E-value: 7.97e-48
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 72 MGKDISYKELDDHSRALAAWLQsRGLVKGDRVAIMMPNILQYPITFTAVLRAGFVVVNVNplYTPRE--LEHQLNDAGAK 149
Cdd:PRK08633 638 TGGELSYGKALTGALALARLLK-RELKDEENVGILLPPSVAGALANLALLLAGKVPVNLN--YTASEaaLKSAIEQAQIK 714
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 150 ALVVLENFATTVEKALPSINVPN----ILVASMGDMLGFKGHIVNLVVRRVkkmVPAWNIpghvrfkdalaqgraKSFNP 225
Cdd:PRK08633 715 TVITSRKFLEKLKNKGFDLELPEnvkvIYLEDLKAKISKVDKLTALLAARL---LPARLL---------------KRLYG 776
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 226 VPVQGCDIAFLQYTGGTTGISKGAMLTHSNILANVEQMNlwmDVaFRNKGKPKALNfvcALPLYHIFALTVNAMIGMKLG 305
Cdd:PRK08633 777 PTFKPDDTATIIFSSGSEGEPKGVMLSHHNILSNIEQIS---DV-FNLRNDDVILS---SLPFFHSFGLTVTLWLPLLEG 849
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 306 ARNILIPNPRDIPSFVKELKKYPVHIFPGLNTLFNGLMNNPDFQTLDFKPLILTLGGGMAVQRPVAERWQHMTGCHITEG 385
Cdd:PRK08633 850 IKVVYHPDPTDALGIAKLVAKHRATILLGTPTFLRLYLRNKKLHPLMFASLRLVVAGAEKLKPEVADAFEEKFGIRILEG 929
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 386 YGLSETSPVACANALD--ATEFT-------GTIGLPMPSTDVVIRD-DDGSDLLLGKVGEICVRGPQVMKGYWNRPEETA 455
Cdd:PRK08633 930 YGATETSPVASVNLPDvlAADFKrqtgskeGSVGMPLPGVAVRIVDpETFEELPPGEDGLILIGGPQVMKGYLGDPEKTA 1009
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 456 ---RAIMADGFFRTGDMGFMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVAAE--HPGVLESAAVGIPNEHSGEvvKLY 530
Cdd:PRK08633 1010 eviKDIDGIGWYVTGDKGHLDEDGFLTITDRYSRFAKIGGEMVPLGAVEEELAKalGGEEVVFAVTAVPDEKKGE--KLV 1087
|
490 500 510 520
....*....|....*....|....*....|....*....|....*
gi 479816261 531 VVRRDPELTEDEVKAFCAE-RLTNYKRPREVEFRESLPKSNVGKI 574
Cdd:PRK08633 1088 VLHTCGAEDVEELKRAIKEsGLPNLWKPSRYFKVEALPLLGSGKL 1132
|
|
| LC-FACS_euk |
cd05927 |
Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS); The members of this family are ... |
76-523 |
5.34e-47 |
|
Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS); The members of this family are eukaryotic fatty acid CoA synthetases that activate fatty acids with chain lengths of 12 to 20. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Organisms tend to have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells.
Pssm-ID: 341250 [Multi-domain] Cd Length: 545 Bit Score: 172.79 E-value: 5.34e-47
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 76 ISYKELDDHSRALAAWLQSRGL--VKGDRVAIMMPNILQYPITFTAVLRAGFVVVnvnPLY---TPRELEHQLNDAGAKA 150
Cdd:cd05927 6 ISYKEVAERADNIGSALRSLGGkpAPASFVGIYSINRPEWIISELACYAYSLVTV---PLYdtlGPEAIEYILNHAEISI 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 151 LVVLENFATtvekalpsinvpnilvasmgdmlgfkghivnlvvrrvkkmvpawnipghVRFKDALAQGRAKSFNPVPVQG 230
Cdd:cd05927 83 VFCDAGVKV-------------------------------------------------YSLEEFEKLGKKNKVPPPPPKP 113
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 231 CDIAFLQYTGGTTGISKGAMLTHSNILANVEQMNLWMDVAFRnkgkpKALNFV--CALPLYHIF---ALTVNAMIGMKLG 305
Cdd:cd05927 114 EDLATICYTSGTTGNPKGVMLTHGNIVSNVAGVFKILEILNK-----INPTDVyiSYLPLAHIFervVEALFLYHGAKIG 188
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 306 arnILIPNPRDIPSFVKELKKYpvhIFPG----------------------LNTLFNGLMN-------------NPDFQT 350
Cdd:cd05927 189 ---FYSGDIRLLLDDIKALKPT---VFPGvprvlnriydkifnkvqakgplKRKLFNFALNyklaelrsgvvraSPFWDK 262
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 351 LDFKPLILTLG--------GGMAVQRPVAERWQHMTGCHITEGYGLSETSPVACANALDATEfTGTIGLPMPSTDVVIRD 422
Cdd:cd05927 263 LVFNKIKQALGgnvrlmltGSAPLSPEVLEFLRVALGCPVLEGYGQTECTAGATLTLPGDTS-VGHVGGPLPCAEVKLVD 341
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 423 --DDGSDLLLGK-VGEICVRGPQVMKGYWNRPEETARAIMADGFFRTGDMGFMDERGYTKIVDRKKDMI-LVSGFNVYPN 498
Cdd:cd05927 342 vpEMNYDAKDPNpRGEVCIRGPNVFSGYYKDPEKTAEALDEDGWLHTGDIGEWLPNGTLKIIDRKKNIFkLSQGEYVAPE 421
|
490 500 510
....*....|....*....|....*....|....*.
gi 479816261 499 EIEEVAAEHPGV---------LESAAVGI--PNEHS 523
Cdd:cd05927 422 KIENIYARSPFVaqifvygdsLKSFLVAIvvPDPDV 457
|
|
| PRK06164 |
PRK06164 |
acyl-CoA synthetase; Validated |
47-581 |
1.53e-46 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 235722 [Multi-domain] Cd Length: 540 Bit Score: 171.46 E-value: 1.53e-46
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 47 SAATSISDMISASCRQFANSPAFTCMGKDISYKELDDHSRALAAWLQSRGLVKGDRVAIMMPNILQYPITFTAVLRAGFV 126
Cdd:PRK06164 7 PRADTLASLLDAHARARPDAVALIDEDRPLSRAELRALVDRLAAWLAAQGVRRGDRVAVWLPNCIEWVVLFLACARLGAT 86
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 127 VVNVNPLYTPRELEHQLNDAGAKALVVLENFAttvekalpSINVPNILVASMGDML-GFKGHIVnlvvrrVKKMVPAwnI 205
Cdd:PRK06164 87 VIAVNTRYRSHEVAHILGRGRARWLVVWPGFK--------GIDFAAILAAVPPDALpPLRAIAV------VDDAADA--T 150
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 206 PGHVRFK-------DALAQGRAKSFNPVPVQGCDIAFLqyTGGTTGISKGAMLTHSNILANVeqmnlwmDVAFRNKGKPK 278
Cdd:PRK06164 151 PAPAPGArvqlfalPDPAPPAAAGERAADPDAGALLFT--TSGTTSGPKLVLHRQATLLRHA-------RAIARAYGYDP 221
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 279 ALNFVCALPLYHIFALtvNAMIGMKLGARNILIPNPRDIPSFVKELKKYPV-HIFPG---LNTLFNGLMNNPDFQTL--- 351
Cdd:PRK06164 222 GAVLLAALPFCGVFGF--STLLGALAGGAPLVCEPVFDAARTARALRRHRVtHTFGNdemLRRILDTAGERADFPSArlf 299
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 352 ---DFKPliltlgggmAVQRPVAERWQHmtGCHITEGYGLSET-SPVACANA-LDATEFTGTIGLPM-PSTDVVIRD-DD 424
Cdd:PRK06164 300 gfaSFAP---------ALGELAALARAR--GVPLTGLYGSSEVqALVALQPAtDPVSVRIEGGGRPAsPEARVRARDpQD 368
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 425 GSDLLLGKVGEICVRGPQVMKGYWNRPEETARAIMADGFFRTGDMGFM-DERGYTkIVDRKKDMILVSGFNVYPNEIEEV 503
Cdd:PRK06164 369 GALLPDGESGEIEIRAPSLMRGYLDNPDATARALTDDGYFRTGDLGYTrGDGQFV-YQTRMGDSLRLGGFLVNPAEIEHA 447
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 504 AAEHPGVLESAAVGIpnEHSGEVVKLYVVRRDP--ELTEDEVKAFCAERLTNYKRPREVEFRESLP---KSNVGKILRRE 578
Cdd:PRK06164 448 LEALPGVAAAQVVGA--TRDGKTVPVAFVIPTDgaSPDEAGLMAACREALAGFKVPARVQVVEAFPvteSANGAKIQKHR 525
|
...
gi 479816261 579 LRD 581
Cdd:PRK06164 526 LRE 528
|
|
| PRK13390 |
PRK13390 |
acyl-CoA synthetase; Provisional |
73-580 |
1.00e-45 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 139538 [Multi-domain] Cd Length: 501 Bit Score: 168.65 E-value: 1.00e-45
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 73 GKDISYKELDDHSRALAAWLQSRGLVKGDRVAIMMPNILQYPITFTAVLRAGFVVVNVNPLYTPRELEHQLNDAGAKALV 152
Cdd:PRK13390 22 GEQVSYRQLDDDSAALARVLYDAGLRTGDVVALLSDNSPEALVVLWAALRSGLYITAINHHLTAPEADYIVGDSGARVLV 101
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 153 V---LENFATTVEKALPSinvpnilvasmgdMLGFKGHIvnlvvrrvkkmvpawniPGHVRFKDALAQGRAksfnPVPVQ 229
Cdd:PRK13390 102 AsaaLDGLAAKVGADLPL-------------RLSFGGEI-----------------DGFGSFEAALAGAGP----RLTEQ 147
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 230 GCDiAFLQYTGGTTGISKGAM--LTHSNILANVEQMnlwMDVAFRNKGKPKALNFVCALPLYHIFALTVNAMIgMKLGAr 307
Cdd:PRK13390 148 PCG-AVMLYSSGTTGFPKGIQpdLPGRDVDAPGDPI---VAIARAFYDISESDIYYSSAPIYHAAPLRWCSMV-HALGG- 221
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 308 NILIPNPRDIPSFVKELKKYPVHIFPGLNTLFNGLMN-NPDFQT-LDFKPLILTLGGGMAVQRPVAERWQHMTGCHITEG 385
Cdd:PRK13390 222 TVVLAKRFDAQATLGHVERYRITVTQMVPTMFVRLLKlDADVRTrYDVSSLRAVIHAAAPCPVDVKHAMIDWLGPIVYEY 301
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 386 YGLSETSPVACANALDATEFTGTIGLPMPStDVVIRDDDGSDLLLGKVGEICVRGPQVMKGYWNRPEETARAIM-ADGFF 464
Cdd:PRK13390 302 YSSTEAHGMTFIDSPDWLAHPGSVGRSVLG-DLHICDDDGNELPAGRIGTVYFERDRLPFRYLNDPEKTAAAQHpAHPFW 380
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 465 RT-GDMGFMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVAAEHPGVLESAAVGIPNEHSGEVVKLYV-----VRRDPEL 538
Cdd:PRK13390 381 TTvGDLGSVDEDGYLYLADRKSFMIISGGVNIYPQETENALTMHPAVHDVAVIGVPDPEMGEQVKAVIqlvegIRGSDEL 460
|
490 500 510 520
....*....|....*....|....*....|....*....|..
gi 479816261 539 TEdEVKAFCAERLTNYKRPREVEFRESLPKSNVGKILRRELR 580
Cdd:PRK13390 461 AR-ELIDYTRSRIAHYKAPRSVEFVDELPRTPTGKLVKGLLR 501
|
|
| PRK04319 |
PRK04319 |
acetyl-CoA synthetase; Provisional |
75-580 |
1.01e-45 |
|
acetyl-CoA synthetase; Provisional
Pssm-ID: 235279 [Multi-domain] Cd Length: 570 Bit Score: 169.69 E-value: 1.01e-45
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 75 DISYKELDDHSRALAAWLQSRGLVKGDRVAIMMPNILQYPITFTAVLRAGFVVvnvNPLYT---PRELEHQLNDAGAKAL 151
Cdd:PRK04319 73 KYTYKELKELSNKFANVLKELGVEKGDRVFIFMPRIPELYFALLGALKNGAIV---GPLFEafmEEAVRDRLEDSEAKVL 149
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 152 VvlenfatTVEKALPSINVPnilvasmgDMLGFKgHIVnLVVRRVKkmvpawNIPGHVRFKDALAQGrAKSFNPVPVQGC 231
Cdd:PRK04319 150 I-------TTPALLERKPAD--------DLPSLK-HVL-LVGEDVE------EGPGTLDFNALMEQA-SDEFDIEWTDRE 205
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 232 DIAFLQYTGGTTGISKGAMLTHSNILANVEQMNLWMDVafrnkgKPKALNFVCALP------LYHIFALTVNamigmklG 305
Cdd:PRK04319 206 DGAILHYTSGSTGKPKGVLHVHNAMLQHYQTGKYVLDL------HEDDVYWCTADPgwvtgtSYGIFAPWLN-------G 272
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 306 ARNILIPNPRDIPSFVKELKKYPVHIFPGLNTLFNGLMNNPD--FQTLDFKPL--ILTLGG---------GMAV-QRPVA 371
Cdd:PRK04319 273 ATNVIDGGRFSPERWYRILEDYKVTVWYTAPTAIRMLMGAGDdlVKKYDLSSLrhILSVGEplnpevvrwGMKVfGLPIH 352
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 372 ER-WQHMTGCHITEGYGLSETSPvacanaldateftGTIGLPMPSTDVVIRDDDGSDLLLGKVGEICVRG--PQVMKGYW 448
Cdd:PRK04319 353 DNwWMTETGGIMIANYPAMDIKP-------------GSMGKPLPGIEAAIVDDQGNELPPNRMGNLAIKKgwPSMMRGIW 419
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 449 NRPEETaRAIMADGFFRTGDMGFMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVAAEHPGVLESAAVGIPNEHSGEVVK 528
Cdd:PRK04319 420 NNPEKY-ESYFAGDWYVSGDSAYMDEDGYFWFQGRVDDVIKTSGERVGPFEVESKLMEHPAVAEAGVIGKPDPVRGEIIK 498
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*.
gi 479816261 529 LYVV-RRDPELTED---EVKAFCAERLTNYKRPREVEFRESLPKSNVGKILRRELR 580
Cdd:PRK04319 499 AFVAlRPGYEPSEElkeEIRGFVKKGLGAHAAPREIEFKDKLPKTRSGKIMRRVLK 554
|
|
| PRK13382 |
PRK13382 |
bile acid CoA ligase; |
76-581 |
2.19e-45 |
|
bile acid CoA ligase;
Pssm-ID: 172019 [Multi-domain] Cd Length: 537 Bit Score: 168.40 E-value: 2.19e-45
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 76 ISYKELDDHSRALAAWLQSRGLVKGDRVAIMMPNILQYPITFTAVLRAGFVVVNVNPLYTPRELEHQLNDAGAKALVVLE 155
Cdd:PRK13382 69 LTWRELDERSDALAAALQALPIGEPRVVGIMCRNHRGFVEALLAANRIGADILLLNTSFAGPALAEVVTREGVDTVIYDE 148
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 156 NFATTVEKALPsiNVPNilvasmgdmlgfkghivnlVVRRVkkmvpAWNIPGHVRFKDALAQGRAKSFNPVPVQGCDIAF 235
Cdd:PRK13382 149 EFSATVDRALA--DCPQ-------------------ATRIV-----AWTDEDHDLTVEVLIAAHAGQRPEPTGRKGRVIL 202
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 236 LqyTGGTTGISKGAmlTHSNIlANVEQMNLWMDVAFRNKGKPkalnFVCALPLYH-------IFALTVNAMIGMKlgaRN 308
Cdd:PRK13382 203 L--TSGTTGTPKGA--RRSGP-GGIGTLKAILDRTPWRAEEP----TVIVAPMFHawgfsqlVLAASLACTIVTR---RR 270
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 309 IlipNPRDIPSFVKELKKYPVHIFPglnTLFNGLMNNPD--FQTLDFKPLILTLGGGMAVQRPVAERWQHMTGCHITEGY 386
Cdd:PRK13382 271 F---DPEATLDLIDRHRATGLAVVP---VMFDRIMDLPAevRNRYSGRSLRFAAASGSRMRPDVVIAFMDQFGDVIYNNY 344
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 387 GLSETSPVACANALDATEFTGTIGLPMPSTDVVIRDDDGSDLLLGKVGEICVRGPQVMKGYwnRPEETARAImaDGFFRT 466
Cdd:PRK13382 345 NATEAGMIATATPADLRAAPDTAGRPAEGTEIRILDQDFREVPTGEVGTIFVRNDTQFDGY--TSGSTKDFH--DGFMAS 420
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 467 GDMGFMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVAAEHPGVLESAAVGIPNEHSGEVVKLYVV-RRDPELTEDEVKA 545
Cdd:PRK13382 421 GDVGYLDENGRLFVVGRDDEMIVSGGENVYPIEVEKTLATHPDVAEAAVIGVDDEQYGQRLAAFVVlKPGASATPETLKQ 500
|
490 500 510
....*....|....*....|....*....|....*.
gi 479816261 546 FCAERLTNYKRPREVEFRESLPKSNVGKILRRELRD 581
Cdd:PRK13382 501 HVRDNLANYKVPRDIVVLDELPRGATGKILRRELQA 536
|
|
| A_NRPS_Cytc1-like |
cd17643 |
similar to adenylation domain of cytotrienin synthetase CytC1; This family of the adenylation ... |
67-579 |
2.43e-45 |
|
similar to adenylation domain of cytotrienin synthetase CytC1; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes Streptomyces sp. cytotrienin synthetase (CytC1), a relatively promiscuous adenylation enzyme that installs the aminoacyl moieties on the phosphopantetheinyl arm of the holo carrier protein CytC2. Also included are Streptomyces sp Thr1, involved in the biosynthesis of 4-chlorothreonine, Pseudomonas aeruginosa pyoverdine synthetase D (PvdD), involved in the biosynthesis of the siderophore pyoverdine and Pseudomonas syringae syringopeptin synthetase, where syringpeptin is a necrosis-inducing phytotoxin that functions as a virulence determinant in the plant-pathogen interaction. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341298 [Multi-domain] Cd Length: 450 Bit Score: 166.33 E-value: 2.43e-45
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 67 PAFTCMGKDISYKELDDHSRALAAWLQSRGLVKGDRVAIMMPNILQYPITFTAVLRAGFVVVNVNPLYTPRELEHQLNDA 146
Cdd:cd17643 4 VAVVDEDRRLTYGELDARANRLARTLRAEGVGPGDRVALALPRSAELIVALLAILKAGGAYVPIDPAYPVERIAFILADS 83
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 147 GAKALVVlenfattvekalpsinvpnilvasmgdmlgfkghivnlvvrrvkkmvpawnipghvrfkdalaqgraksfnpv 226
Cdd:cd17643 84 GPSLLLT------------------------------------------------------------------------- 90
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 227 pvQGCDIAFLQYTGGTTGISKGAMLTHSNILANVEQMNLWM-----DVAFrnkgkpkalnfvcalpLYHIFAL--TVNAM 299
Cdd:cd17643 91 --DPDDLAYVIYTSGSTGRPKGVVVSHANVLALFAATQRWFgfnedDVWT----------------LFHSYAFdfSVWEI 152
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 300 IGMKL-GARNILIPN-----PRDIPSFVKELKkypVHIFPGLNTLFNGLMNNPDFQTLDFKPLILTLGGGMAVQRPVAER 373
Cdd:cd17643 153 WGALLhGGRLVVVPYevarsPEDFARLLRDEG---VTVLNQTPSAFYQLVEAADRDGRDPLALRYVIFGGEALEAAMLRP 229
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 374 W-QHM--TGCHITEGYGLSETSPVACANALDATEFTG----TIGLPMPSTDVVIRDDDGSDLLLGKVGEICVRGPQVMKG 446
Cdd:cd17643 230 WaGRFglDRPQLVNMYGITETTVHVTFRPLDAADLPAaaasPIGRPLPGLRVYVLDADGRPVPPGVVGELYVSGAGVARG 309
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 447 YWNRPEETARAIMADGF-------FRTGDMGFMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVAAEHPGVLESAAVGIP 519
Cdd:cd17643 310 YLGRPELTAERFVANPFggpgsrmYRTGDLARRLPDGELEYLGRADEQVKIRGFRIELGEIEAALATHPSVRDAAVIVRE 389
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|.
gi 479816261 520 NEHSGEVVKLYVVRRD-PELTEDEVKAFCAERLTNYKRPREVEFRESLPKSNVGKILRREL 579
Cdd:cd17643 390 DEPGDTRLVAYVVADDgAAADIAELRALLKELLPDYMVPARYVPLDALPLTVNGKLDRAAL 450
|
|
| PRK08279 |
PRK08279 |
long-chain-acyl-CoA synthetase; Validated |
14-557 |
2.79e-45 |
|
long-chain-acyl-CoA synthetase; Validated
Pssm-ID: 236217 [Multi-domain] Cd Length: 600 Bit Score: 168.90 E-value: 2.79e-45
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 14 DSAKVAPtgeARPAVEKIWLKSYPAGVPhdINLSAATSISDMISASCRQFANSPAFTCMGKDISYKELDDHSRALAAWLQ 93
Cdd:PRK08279 6 DLAARLP---RRLPDLPGILRGLKRTAL--ITPDSKRSLGDVFEEAAARHPDRPALLFEDQSISYAELNARANRYAHWAA 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 94 SRGLVKGDRVAIMMPNILQYPITFTAVLRAGFVVVNVNPLYTPRELEHQLNDAGAKALVVLENFATTVEKALPSINVPNI 173
Cdd:PRK08279 81 ARGVGKGDVVALLMENRPEYLAAWLGLAKLGAVVALLNTQQRGAVLAHSLNLVDAKHLIVGEELVEAFEEARADLARPPR 160
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 174 LVASMGDMLGFKGHIVNLvvrrvkkmvpawnipghvrfkDALAQGRAkSFNPV---PVQGCDIAFLQYTGGTTGISKGAM 250
Cdd:PRK08279 161 LWVAGGDTLDDPEGYEDL---------------------AAAAAGAP-TTNPAsrsGVTAKDTAFYIYTSGTTGLPKAAV 218
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 251 LTHSNILanveqmnLWMDVaFrnkGKPKALN----FVCALPLYHIFALTV----------NAMIGMKLGARNilipnprd 316
Cdd:PRK08279 219 MSHMRWL-------KAMGG-F---GGLLRLTpddvLYCCLPLYHNTGGTVawssvlaagaTLALRRKFSASR-------- 279
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 317 ipsFVKELKKYPVHIFPGLNTLFNGLMNNPDfQTLDFK-PLILTLGGGMavqRP-VAERWQHMTGC-HITEGYGLSEtSP 393
Cdd:PRK08279 280 ---FWDDVRRYRATAFQYIGELCRYLLNQPP-KPTDRDhRLRLMIGNGL---RPdIWDEFQQRFGIpRILEFYAASE-GN 351
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 394 VACANALdatEFTGTIG-LPMPS------------TDVVIRDDDG---------SDLLLGKVGEicvRGPqvMKGYwNRP 451
Cdd:PRK08279 352 VGFINVF---NFDGTVGrVPLWLahpyaivkydvdTGEPVRDADGrcikvkpgeVGLLIGRITD---RGP--FDGY-TDP 422
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 452 EETARAIMADGF------FRTGDMGFMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVAAEHPGVLESAAVG--IPNeHS 523
Cdd:PRK08279 423 EASEKKILRDVFkkgdawFNTGDLMRDDGFGHAQFVDRLGDTFRWKGENVATTEVENALSGFPGVEEAVVYGveVPG-TD 501
|
570 580 590
....*....|....*....|....*....|....*.
gi 479816261 524 GE--VVKLyVVRRDPELTEDEVKAFCAERLTNYKRP 557
Cdd:PRK08279 502 GRagMAAI-VLADGAEFDLAALAAHLYERLPAYAVP 536
|
|
| A_NRPS_Ta1_like |
cd12116 |
The adenylation domain of nonribosomal peptide synthetases (NRPS), including salinosporamide A ... |
65-579 |
3.10e-45 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS), including salinosporamide A polyketide synthase; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the myxovirescin (TA) antibiotic biosynthetic gene in Myxococcus xanthus; TA production plays a role in predation. It also includes the salinosporamide A polyketide synthase which is involved in the biosynthesis of salinosporamide A, a marine microbial metabolite whose chlorine atom is crucial for potent proteasome inhibition and anticancer activity.
Pssm-ID: 341281 [Multi-domain] Cd Length: 470 Bit Score: 166.31 E-value: 3.10e-45
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 65 NSPAFTCMGKDISYKELDDHSRALAAWLQSRGLVKGDRVAIMMPNILQYPITFTAVLRAGFVVVNVNPLYtPRE-LEHQL 143
Cdd:cd12116 2 DATAVRDDDRSLSYAELDERANRLAARLRARGVGPGDRVAVYLPRSARLVAAMLAVLKAGAAYVPLDPDY-PADrLRYIL 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 144 NDAGAKALVVlenfattvEKALPsinvpnilvasmgdmlgfkgHIVNLVVRRVKKMVPAWNIPGHVRfkdalaqgraksf 223
Cdd:cd12116 81 EDAEPALVLT--------DDALP--------------------DRLPAGLPVLLLALAAAAAAPAAP------------- 119
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 224 nPVPVQGCDIAFLQYTGGTTGISKGAMLTHSNILANVEQMNlwMDVAFRNKGKPKALNFVC--------ALPLYH----I 291
Cdd:cd12116 120 -RTPVSPDDLAYVIYTSGSTGRPKGVVVSHRNLVNFLHSMR--ERLGLGPGDRLLAVTTYAfdisllelLLPLLAgarvV 196
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 292 FALTVNAMIGMKLGArniLIPNPR-----DIPSFVKELKKYPvhiFPGLNTLfnglmnnpdfqTLdfkpliltLGGGMAV 366
Cdd:cd12116 197 IAPRETQRDPEALAR---LIEAHSitvmqATPATWRMLLDAG---WQGRAGL-----------TA--------LCGGEAL 251
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 367 QRPVAERWQHMTGChITEGYGLSETSPVACANALDATEFTGTIGLPMPSTDVVIRDDDGSDLLLGKVGEICVRGPQVMKG 446
Cdd:cd12116 252 PPDLAARLLSRVGS-LWNLYGPTETTIWSTAARVTAAAGPIPIGRPLANTQVYVLDAALRPVPPGVPGELYIGGDGVAQG 330
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 447 YWNRPEETARAIMADGF-------FRTGDMGFMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVAAEHPGVLESAAVGIP 519
Cdd:cd12116 331 YLGRPALTAERFVPDPFagpgsrlYRTGDLVRRRADGRLEYLGRADGQVKIRGHRIELGEIEAALAAHPGVAQAAVVVRE 410
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 520 NEHSGEVVKLYVVRRDPELTEDEVKAFCAERLTNYKRPREVEFRESLPKSNVGKILRREL 579
Cdd:cd12116 411 DGGDRRLVAYVVLKAGAAPDAAALRAHLRATLPAYMVPSAFVRLDALPLTANGKLDRKAL 470
|
|
| PRK05852 |
PRK05852 |
fatty acid--CoA ligase family protein; |
48-577 |
1.00e-44 |
|
fatty acid--CoA ligase family protein;
Pssm-ID: 235625 [Multi-domain] Cd Length: 534 Bit Score: 166.22 E-value: 1.00e-44
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 48 AATSISDMISASCRQFANSPA--FTCMGKDISYKELDDHSRALAAWLQSRGLVKGDRVAIMMPNILQYPITFTAVLRAGF 125
Cdd:PRK05852 14 FGPRIADLVEVAATRLPEAPAlvVTADRIAISYRDLARLVDDLAGQLTRSGLLPGDRVALRMGSNAEFVVALLAASRADL 93
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 126 VVVNVNPLYTPRELEHQLNDAGAKalVVLENFATTVEKALPSINVPNILVaSMGDMLGFKGHIVNLVVRRVKKMVPAWNI 205
Cdd:PRK05852 94 VVVPLDPALPIAEQRVRSQAAGAR--VVLIDADGPHDRAEPTTRWWPLTV-NVGGDSGPSGGTLSVHLDAATEPTPATST 170
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 206 PGHVRFKDALaqgraksfnpvpvqgcdiafLQYTGGTTGISKGAMLTHSNILANVEQMnlwmdVAFRNKGKPKALnfVCA 285
Cdd:PRK05852 171 PEGLRPDDAM--------------------IMFTGGTTGLPKMVPWTHANIASSVRAI-----ITGYRLSPRDAT--VAV 223
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 286 LPLYHIFALtVNAMIGMKLGARNILIPNPRDIP--SFVKELKKYPVHIFPGLNTLFNGLMNNPDFQTLDFK--PLILTLG 361
Cdd:PRK05852 224 MPLYHGHGL-IAALLATLASGGAVLLPARGRFSahTFWDDIKAVGATWYTAVPTIHQILLERAATEPSGRKpaALRFIRS 302
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 362 GGMAVQRPVAERWQHMTGCHITEGYGLSETSPVACANALDATEFT----GTIGLPMPSTDVVIR--DDDGSDLLLGKVGE 435
Cdd:PRK05852 303 CSAPLTAETAQALQTEFAAPVVCAFGMTEATHQVTTTQIEGIGQTenpvVSTGLVGRSTGAQIRivGSDGLPLPAGAVGE 382
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 436 ICVRGPQVMKGYWNRPEETArAIMADGFFRTGDMGFMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVAAEHPGVLESAA 515
Cdd:PRK05852 383 VWLRGTTVVRGYLGDPTITA-ANFTDGWLRTGDLGSLSAAGDLSIRGRIKELINRGGEKISPERVEGVLASHPNVMEAAV 461
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|...
gi 479816261 516 VGIPNEHSGEVVKLYVVRRDP-ELTEDEVKAFCAERLTNYKRPREVEFRESLPKSNVGKILRR 577
Cdd:PRK05852 462 FGVPDQLYGEAVAAVIVPRESaPPTAEELVQFCRERLAAFEIPASFQEASGLPHTAKGSLDRR 524
|
|
| OSB_MenE-like |
cd17630 |
O-succinylbenzoic acid-CoA ligase; This family contains O-succinylbenzoyl-CoA (OSB-CoA) ... |
232-581 |
6.35e-44 |
|
O-succinylbenzoic acid-CoA ligase; This family contains O-succinylbenzoyl-CoA (OSB-CoA) synthetase (also known as O-succinylbenzoic acid CoA ligase) that belongs to the ANL superfamily and catalyzes the ligation of CoA to o-succinylbenzoate (OSB). It includes MenE in the bacterial menaquinone biosynthesis pathway which is a promising target for the development of novel antibacterial agents. MenE catalyzes CoA ligation via an acyl-adenylate intermediate; tight-binding inhibitors of MenE based on stable acyl-sulfonyladenosine analogs of this intermediate provide a pathway toward the development of optimized MenE inhibitors.
Pssm-ID: 341285 [Multi-domain] Cd Length: 325 Bit Score: 159.03 E-value: 6.35e-44
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 232 DIAFLQYTGGTTGISKGAMLTHSNILANVEQMNLWMdvAFRNKGkpkalNFVCALPLYHIFALTVnaMIGMKLGARNILI 311
Cdd:cd17630 1 RLATVILTSGSTGTPKAVVHTAANLLASAAGLHSRL--GFGGGD-----SWLLSLPLYHVGGLAI--LVRSLLAGAELVL 71
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 312 PNPRDiPSFVKELKKYPVHIF---PGLNTLFNGLMNNPDFQTLDfkplILTLGGGmAVQRPVAERWQHMtGCHITEGYGL 388
Cdd:cd17630 72 LERNQ-ALAEDLAPPGVTHVSlvpTQLQRLLDSGQGPAALKSLR----AVLLGGA-PIPPELLERAADR-GIPLYTTYGM 144
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 389 SETSPVACANALDATEfTGTIGLPMPSTDVVIRDDdgsdlllgkvGEICVRGPQVMKGYWNRPEETARAimADGFFRTGD 468
Cdd:cd17630 145 TETASQVATKRPDGFG-RGGVGVLLPGRELRIVED----------GEIWVGGASLAMGYLRGQLVPEFN--EDGWFTTKD 211
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 469 MGFMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVAAEHPGVLESAAVGIPNEHSGE-VVKLYVVRrdPELTEDEVKAFC 547
Cdd:cd17630 212 LGELHADGRLTVLGRADNMIISGGENIQPEEIEAALAAHPAVRDAFVVGVPDEELGQrPVAVIVGR--GPADPAELRAWL 289
|
330 340 350
....*....|....*....|....*....|....
gi 479816261 548 AERLTNYKRPREVEFRESLPKSNVGKILRRELRD 581
Cdd:cd17630 290 KDKLARFKLPKRIYPVPELPRTGGGKVDRRALRA 323
|
|
| MACS_like_2 |
cd05973 |
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the ... |
76-580 |
8.72e-44 |
|
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. MACS enzymes are localized to mitochondria.
Pssm-ID: 341277 [Multi-domain] Cd Length: 437 Bit Score: 161.92 E-value: 8.72e-44
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 76 ISYKELDDHSRALAAWLQSRGLVKGDRVAIMMPNILQYPITFTAVLRAGFVVVnvnPLYT---PRELEHQLNDAGAKALV 152
Cdd:cd05973 1 LTFGELRALSARFANALQELGVGPGDVVAGLLPRTPELVVTILGIWRLGAVYQ---PLFTafgPKAIEHRLRTSGARLVV 77
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 153 VlenfattvekalpsiNVPNilvasmgdmlgfkghivnlvvrrvkkmvpawnipghvrfKDALAQgraksfnpvpvqgcD 232
Cdd:cd05973 78 T---------------DAAN---------------------------------------RHKLDS--------------D 89
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 233 IAFLQYTGGTTGISKGAMLTHSNILANVEQMNLWMDV----AFRNKGKPKalnfvCALPLYHifaltvnAMIG-MKLGAR 307
Cdd:cd05973 90 PFVMMFTSGTTGLPKGVPVPLRALAAFGAYLRDAVDLrpedSFWNAADPG-----WAYGLYY-------AITGpLALGHP 157
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 308 NILIPNPRDIPSFVKELKKYPVHIFPGLNTLFNGLMNNPdfqtldfKPLILTLGGGMAVQRPVAE-------RWQHMT-G 379
Cdd:cd05973 158 TILLEGGFSVESTWRVIERLGVTNLAGSPTAYRLLMAAG-------AEVPARPKGRLRRVSSAGEpltpeviRWFDAAlG 230
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 380 CHITEGYGLSETS-PVACANALDATEFTGTIGLPMPSTDVVIRDDDGSDLLLGKVGEICV---RGPQV-MKGYWNRPEET 454
Cdd:cd05973 231 VPIHDHYGQTELGmVLANHHALEHPVHAGSAGRAMPGWRVAVLDDDGDELGPGEPGRLAIdiaNSPLMwFRGYQLPDTPA 310
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 455 AraimADGFFRTGDMGFMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVAAEHPGVLESAAVGIPNEHSGEVVKLYVVRR 534
Cdd:cd05973 311 I----DGGYYLTGDTVEFDPDGSFSFIGRADDVITMSGYRIGPFDVESALIEHPAVAEAAVIGVPDPERTEVVKAFVVLR 386
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|.
gi 479816261 535 D-----PELtEDEVKAFCAERLTNYKRPREVEFRESLPKSNVGKILRRELR 580
Cdd:cd05973 387 GghegtPAL-ADELQLHVKKRLSAHAYPRTIHFVDELPKTPSGKIQRFLLR 436
|
|
| ACS-like |
cd17634 |
acetate-CoA ligase; This family includes acyl- and aryl-CoA ligases, as well as the ... |
74-575 |
1.67e-43 |
|
acetate-CoA ligase; This family includes acyl- and aryl-CoA ligases, as well as the adenylation domain of nonribosomal peptide synthetases and firefly luciferases. The adenylate-forming enzymes catalyze an ATP-dependent two-step reaction to first activate a carboxylate substrate as an adenylate and then transfer the carboxylate to the pantetheine group of either coenzyme A or an acyl-carrier protein. The active site of the domain is located at the interface of a large N-terminal subdomain and a smaller C-terminal subdomain.
Pssm-ID: 341289 [Multi-domain] Cd Length: 587 Bit Score: 163.90 E-value: 1.67e-43
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 74 KDISYKELDDHSRALAAWLQSRGLVKGDRVAIMMPNILQYPITFTAVLRAGFVVVNVNPLYTPRELEHQLNDAGAKALVV 153
Cdd:cd17634 83 RTISYRELHREVCRFAGTLLDLGVKKGDRVAIYMPMIPEAAVAMLACARIGAVHSVIFGGFAPEAVAGRIIDSSSRLLIT 162
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 154 LENFATtvekalPSINVPniLVASMGDMLGFKGHIVNLVVRRVKKMVPAWNIPGHVRFKDALAQGRAKSFNPVPVQGCDI 233
Cdd:cd17634 163 ADGGVR------AGRSVP--LKKNVDDALNPNVTSVEHVIVLKRTGSDIDWQEGRDLWWRDLIAKASPEHQPEAMNAEDP 234
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 234 AFLQYTGGTTGISKGAMLTHSNILANVEQM----------NLWMDVAfrnkgkpkALNFVCALPlYHIFAltvnamiGMK 303
Cdd:cd17634 235 LFILYTSGTTGKPKGVLHTTGGYLVYAATTmkyvfdygpgDIYWCTA--------DVGWVTGHS-YLLYG-------PLA 298
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 304 LGARNIL---IPNPRDIPSFVKELKKYPVHIFPGLNTLFNGLM-NNPD-FQTLDFKPLiLTLGGGMAVQRPVAERW--QH 376
Cdd:cd17634 299 CGATTLLyegVPNWPTPARMWQVVDKHGVNILYTAPTAIRALMaAGDDaIEGTDRSSL-RILGSVGEPINPEAYEWywKK 377
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 377 MTG--CHITEGYGLSETSPVACANALDATEF-TGTIGLPMPSTDVVIRDDDGSDLLLGKVGEICVRG--PQVMKGYWNRP 451
Cdd:cd17634 378 IGKekCPVVDTWWQTETGGFMITPLPGAIELkAGSATRPVFGVQPAVVDNEGHPQPGGTEGNLVITDpwPGQTRTLFGDH 457
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 452 EETARAIMA--DGFFRTGDMGFMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVAAEHPGVLESAAVGIPNEHSGEVVKL 529
Cdd:cd17634 458 ERFEQTYFStfKGMYFSGDGARRDEDGYYWITGRSDDVINVAGHRLGTAEIESVLVAHPKVAEAAVVGIPHAIKGQAPYA 537
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|
gi 479816261 530 YVVRR----DPELTEDEVKAFCAERLTNYKRPREVEFRESLPKSNVGKIL 575
Cdd:cd17634 538 YVVLNhgvePSPELYAELRNWVRKEIGPLATPDVVHWVDSLPKTRSGKIM 587
|
|
| A_NRPS_GliP_like |
cd17653 |
nonribosomal peptide synthase GliP-like; This family includes the adenylation (A) domain of ... |
54-581 |
1.84e-43 |
|
nonribosomal peptide synthase GliP-like; This family includes the adenylation (A) domain of nonribosomal peptide synthases (NRPS) gliotoxin biosynthesis protein P (GliP), thioclapurine biosynthesis protein P (tcpP) and Sirodesmin biosynthesis protein P (SirP). In the filamentous fungus Aspergillus fumigatus, NRPS GliP is involved in the biosynthesis of gliotoxin, which is initiated by the condensation of serine and phenylalanine. Studies show that GliP is not required for invasive aspergillosis, suggesting that the principal targets of gliotoxin are neutrophils or other phagocytes. SirP is a phytotoxin produced by the fungus Leptosphaeria maculans, which causes blackleg disease of canola (Brassica napus). In the fungus Claviceps purpurea, NRPS tcpP catalyzes condensation of tyrosine and glycine, part of biosynthesis of an unusual class of epipolythiodioxopiperazines (ETPs) that lacks the reactive thiol group for toxicity. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341308 [Multi-domain] Cd Length: 433 Bit Score: 160.55 E-value: 1.84e-43
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 54 DMISASCRQFANSPAFTCMGKDISYKELDDHSRALAAWLQSRGLVKGDRVAIMMPNILQYPITFTAVLRAGFVVVNVNPL 133
Cdd:cd17653 1 DAFERIAAAHPDAVAVESLGGSLTYGELDAASNALANRLLQLGVVPGDVVPLLSDRSLEMLVAILAILKAGAAYVPLDAK 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 134 YTPRELEHQLNDAGAKALVvlenFATTVEkalpsinvpnilvasmgdmlgfkghivnlvvrrvkkmvpawnipghvrfkd 213
Cdd:cd17653 81 LPSARIQAILRTSGATLLL----TTDSPD--------------------------------------------------- 105
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 214 alaqgraksfnpvpvqgcDIAFLQYTGGTTGISKGAMLTHSNILANVEQMNLWMDVafrNKGKPKAlnfvcalplyHIFA 293
Cdd:cd17653 106 ------------------DLAYIIFTSGSTGIPKGVMVPHRGVLNYVSQPPARLDV---GPGSRVA----------QVLS 154
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 294 LTVNAMIGMKLGA---RNILIPnpRDIPSFVKELKKyPVHIFPGLNTLFNGLmnNP-DFQTLDfkpLILTlgGGMAVQRP 369
Cdd:cd17653 155 IAFDACIGEIFSTlcnGGTLVL--ADPSDPFAHVAR-TVDALMSTPSILSTL--SPqDFPNLK---TIFL--GGEAVPPS 224
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 370 VAERWQHmtGCHITEGYGLSETSPVACANALDATEFTgTIGLPMPSTDVVIRDDDGSDLLLGKVGEICVRGPQVMKGYWN 449
Cdd:cd17653 225 LLDRWSP--GRRLYNAYGPTECTISSTMTELLPGQPV-TIGKPIPNSTCYILDADLQPVPEGVVGEICISGVQVARGYLG 301
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 450 RPEETARAIMADGF------FRTGDMGFMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVAAEHPGVLESAAVGIpneHS 523
Cdd:cd17653 302 NPALTASKFVPDPFwpgsrmYRTGDYGRWTEDGGLEFLGREDNQVKVRGFRINLEEIEEVVLQSQPEVTQAAAIV---VN 378
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*....
gi 479816261 524 GEVVKLYVvrrdPE-LTEDEVKAFCAERLTNYKRPREVEFRESLPKSNVGKILRRELRD 581
Cdd:cd17653 379 GRLVAFVT----PEtVDVDGLRSELAKHLPSYAVPDRIIALDSFPLTANGKVDRKALRE 433
|
|
| PRK07638 |
PRK07638 |
acyl-CoA synthetase; Validated |
76-580 |
3.56e-43 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236071 [Multi-domain] Cd Length: 487 Bit Score: 161.10 E-value: 3.56e-43
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 76 ISYKELDDHSRALAAWLQSRGlVKGDRVAIMMPNILQYPITFTAVLRAGFVVVNVNPLYTPRELEHQLNDAGAkALVVLE 155
Cdd:PRK07638 27 LTYKDWFESVCKVANWLNEKE-SKNKTIAILLENRIEFLQLFAGAAMAGWTCVPLDIKWKQDELKERLAISNA-DMIVTE 104
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 156 NFattvekalpsinvpnilvaSMGDMLGFKGHIVNLvvRRVKKMVPAwnipghvrfkdalaqgraksFNPVPVQGCDIA- 234
Cdd:PRK07638 105 RY-------------------KLNDLPDEEGRVIEI--DEWKRMIEK--------------------YLPTYAPIENVQn 143
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 235 ---FLQYTGGTTGISKGAMLTHSNilanveqmnlWMDV------AFRNKGKPKAL---NFVCALPLYhifaltvNAMIGM 302
Cdd:PRK07638 144 apfYMGFTSGSTGKPKAFLRAQQS----------WLHSfdcnvhDFHMKREDSVLiagTLVHSLFLY-------GAISTL 206
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 303 KLGARNILIPNprDIPSFVKE-LKKYPVHIFPGLNTLFNGLMNNPDFqtLDFKPLILTLG---GGMAVQRpVAERWQHMT 378
Cdd:PRK07638 207 YVGQTVHLMRK--FIPNQVLDkLETENISVMYTVPTMLESLYKENRV--IENKMKIISSGakwEAEAKEK-IKNIFPYAK 281
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 379 gchITEGYGLSETSPVACANALDATEFTGTIGLPMPSTDVVIRDDDGSDLLLGKVGEICVRGPQVMKGYWNRpEETARAI 458
Cdd:PRK07638 282 ---LYEFYGASELSFVTALVDEESERRPNSVGRPFHNVQVRICNEAGEEVQKGEIGTVYVKSPQFFMGYIIG-GVLAREL 357
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 459 MADGFFRTGDMGFMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVAAEHPGVLESAAVGIPNEHSGEVVKLYVvrrDPEL 538
Cdd:PRK07638 358 NADGWMTVRDVGYEDEEGFIYIVGREKNMILFGGINIFPEEIESVLHEHPAVDEIVVIGVPDSYWGEKPVAII---KGSA 434
|
490 500 510 520
....*....|....*....|....*....|....*....|..
gi 479816261 539 TEDEVKAFCAERLTNYKRPREVEFRESLPKSNVGKILRRELR 580
Cdd:PRK07638 435 TKQQLKSFCLQRLSSFKIPKEWHFVDEIPYTNSGKIARMEAK 476
|
|
| AACS_like |
cd05968 |
Uncharacterized acyl-CoA synthetase subfamily similar to Acetoacetyl-CoA synthetase; This ... |
67-580 |
1.69e-42 |
|
Uncharacterized acyl-CoA synthetase subfamily similar to Acetoacetyl-CoA synthetase; This uncharacterized acyl-CoA synthetase family (EC 6.2.1.16, or acetoacetate#CoA ligase or acetoacetate:CoA ligase (AMP-forming)) is highly homologous to acetoacetyl-CoA synthetase. However, the proteins in this family exist in only bacteria and archaea. AACS is a cytosolic ligase that specifically activates acetoacetate to its coenzyme A ester by a two-step reaction. Acetoacetate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is the first step of the mevalonate pathway of isoprenoid biosynthesis via isopentenyl diphosphate. Isoprenoids are a large class of compounds found in all living organisms.
Pssm-ID: 341272 [Multi-domain] Cd Length: 610 Bit Score: 161.50 E-value: 1.69e-42
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 67 PAFTCMGKD-----ISYKELDDHSRALAAWLQSRGLVKGDRVAIMMPNILQYPITFTAVLRAGFVVVNVNPLYTPRELEH 141
Cdd:cd05968 78 PALRWEGEDgtsrtLTYGELLYEVKRLANGLRALGVGKGDRVGIYLPMIPEIVPAFLAVARIGGIVVPIFSGFGKEAAAT 157
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 142 QLNDAGAKALVVLENFAttvEKALPsINVPNILVASMGDMLGFKgHIVnlVVRRVKKMVPAwnIPGHVRFKDALAQGRAK 221
Cdd:cd05968 158 RLQDAEAKALITADGFT---RRGRE-VNLKEEADKACAQCPTVE-KVV--VVRHLGNDFTP--AKGRDLSYDEEKETAGD 228
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 222 SFNPVPVQgcDIAFLQYTGGTTGISKGAMLTHSNI-LANVEQMNLWMDVafrnkGKPKALNFVCAL-----PLYHIFALT 295
Cdd:cd05968 229 GAERTESE--DPLMIIYTSGTTGKPKGTVHVHAGFpLKAAQDMYFQFDL-----KPGDLLTWFTDLgwmmgPWLIFGGLI 301
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 296 VNAMIGMKLGARNIliPNPRDIPSFVKELKkyPVHIfpGLN-TLFNGLMNNPD--FQTLDFKPLILtLGGGMAVQRPVAE 372
Cdd:cd05968 302 LGATMVLYDGAPDH--PKADRLWRMVEDHE--ITHL--GLSpTLIRALKPRGDapVNAHDLSSLRV-LGSTGEPWNPEPW 374
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 373 RWQHMT----GCHITEGYGLSETSPVACANAL----DATEFTGtiglPMPSTDVVIRDDDGSDLLlGKVGEICVRGPQV- 443
Cdd:cd05968 375 NWLFETvgkgRNPIINYSGGTEISGGILGNVLikpiKPSSFNG----PVPGMKADVLDESGKPAR-PEVGELVLLAPWPg 449
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 444 -MKGYWNRPEETARAIMA--DGFFRTGDMGFMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVAAEHPGVLESAAVGIPN 520
Cdd:cd05968 450 mTRGFWRDEDRYLETYWSrfDNVWVHGDFAYYDEEGYFYILGRSDDTINVAGKRVGPAEIESVLNAHPAVLESAAIGVPH 529
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....
gi 479816261 521 EHSGEVVKLYVVRRD----PELTEDEVKAFCAERLTNYKRPREVEFRESLPKSNVGKILRRELR 580
Cdd:cd05968 530 PVKGEAIVCFVVLKPgvtpTEALAEELMERVADELGKPLSPERILFVKDLPKTRNAKVMRRVIR 593
|
|
| LC_FACS_bac |
cd05932 |
Bacterial long-chain fatty acid CoA synthetase (LC-FACS), including Marinobacter ... |
75-510 |
2.34e-42 |
|
Bacterial long-chain fatty acid CoA synthetase (LC-FACS), including Marinobacter hydrocarbonoclasticus isoprenoid Coenzyme A synthetase; The members of this family are bacterial long-chain fatty acid CoA synthetase. Marinobacter hydrocarbonoclasticus isoprenoid Coenzyme A synthetase in this family is involved in the synthesis of isoprenoid wax ester storage compounds when grown on phytol as the sole carbon source. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.
Pssm-ID: 341255 [Multi-domain] Cd Length: 508 Bit Score: 159.17 E-value: 2.34e-42
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 75 DISYKELDDHSRALAAWLQSRGLVKGDRVAIMMPNILQYPITFTAVLRAGFVVVNVNPLYTPRELEHQLNDAGAKALVV- 153
Cdd:cd05932 6 EFTWGEVADKARRLAAALRALGLEPGSKIALISKNCAEWFITDLAIWMAGHISVPLYPTLNPDTIRYVLEHSESKALFVg 85
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 154 ----LENFATTVEKALPSINVPnilvasmgdmlgfkghivnlvvrrvkkmvPAWNIPGHVRFKDALAQGRAKSFNPVPVQ 229
Cdd:cd05932 86 klddWKAMAPGVPEGLISISLP-----------------------------PPSAANCQYQWDDLIAQHPPLEERPTRFP 136
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 230 GcDIAFLQYTGGTTGISKGAMLTHSNILANVEQ-MNLwmdvaFRNKGKPKALNFvcaLPLYHIFALTVNAMIGMKLGARN 308
Cdd:cd05932 137 E-QLATLIYTSGTTGQPKGVMLTFGSFAWAAQAgIEH-----IGTEENDRMLSY---LPLAHVTERVFVEGGSLYGGVLV 207
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 309 ILipnPRDIPSFVKELKKYPVHIF---PGLNTLFN-GLMNNPDFQTLDFK---PLILTL-----------------GGGM 364
Cdd:cd05932 208 AF---AESLDTFVEDVQRARPTLFfsvPRLWTKFQqGVQDKIPQQKLNLLlkiPVVNSLvkrkvlkglgldqcrlaGCGS 284
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 365 AVQRPVAERWQHMTGCHITEGYGLSETSPVACANaLDATEFTGTIGLPMPSTDVVIRDDdgsdlllgkvGEICVRGPQVM 444
Cdd:cd05932 285 APVPPALLEWYRSLGLNILEAYGMTENFAYSHLN-YPGRDKIGTVGNAGPGVEVRISED----------GEILVRSPALM 353
|
410 420 430 440 450 460
....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 479816261 445 KGYWNRPEETARAIMADGFFRTGDMGFMDERGYTKIVDRKKDMILVS-GFNVYPNEIEEVAAEHPGV 510
Cdd:cd05932 354 MGYYKDPEATAEAFTADGFLRTGDKGELDADGNLTITGRVKDIFKTSkGKYVAPAPIENKLAEHDRV 420
|
|
| PrpE |
cd05967 |
Propionyl-CoA synthetase (PrpE); EC 6.2.1.17: propanoate:CoA ligase (AMP-forming) or ... |
76-581 |
2.39e-42 |
|
Propionyl-CoA synthetase (PrpE); EC 6.2.1.17: propanoate:CoA ligase (AMP-forming) or propionate#CoA ligase (PrpE) catalyzes the first step of the 2-methylcitric acid cycle for propionate catabolism. It activates propionate to propionyl-CoA in a two-step reaction, which proceeds through a propionyl-AMP intermediate and requires ATP and Mg2+. In Salmonella enterica, the PrpE protein is required for growth of Salmonella enterica on propionate and can substitute for the acetyl-CoA synthetase (Acs) enzyme during growth on acetate. PrpE can also activate acetate, 3HP, and butyrate to their corresponding CoA-thioesters, although with less efficiency.
Pssm-ID: 341271 [Multi-domain] Cd Length: 617 Bit Score: 160.94 E-value: 2.39e-42
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 76 ISYKELDDHSRALAAWLQSRGLVKGDRVAIMMPNILQYPITFTAVLRAGFVVVNVNPLYTPRELEHQLNDAGAKaLVVLE 155
Cdd:cd05967 83 YTYAELLDEVSRLAGVLRKLGVVKGDRVIIYMPMIPEAAIAMLACARIGAIHSVVFGGFAAKELASRIDDAKPK-LIVTA 161
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 156 NFATTVEKALPSINvpniLVASMGDMLGFKGHIVnLVVRRVKKMVPAWNIPGHVRFKDALAQgrAKSFNPVPVQGCDIAF 235
Cdd:cd05967 162 SCGIEPGKVVPYKP----LLDKALELSGHKPHHV-LVLNRPQVPADLTKPGRDLDWSELLAK--AEPVDCVPVAATDPLY 234
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 236 LQYTGGTTGISKG---------AMLTHS-NILANVEQMNLWM---DVafrnkGKPKALNFVCALPLYHifaltvnamigm 302
Cdd:cd05967 235 ILYTSGTTGKPKGvvrdngghaVALNWSmRNIYGIKPGDVWWaasDV-----GWVVGHSYIVYGPLLH------------ 297
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 303 klGARNIL-------IPNP----RDI-----------PSFVKELKKYPVHIFPG-------LNTLFngLMNNP-DFQTLD 352
Cdd:cd05967 298 --GATTVLyegkpvgTPDPgafwRVIekyqvnalftaPTAIRAIRKEDPDGKYIkkydlssLRTLF--LAGERlDPPTLE 373
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 353 FKPLILtlgggmavQRPVAER-WQHMTGCHITEGYGLSETSPVAcanaldatefTGTIGLPMPSTDVVIRDDDGSDLLLG 431
Cdd:cd05967 374 WAENTL--------GVPVIDHwWQTETGWPITANPVGLEPLPIK----------AGSPGKPVPGYQVQVLDEDGEPVGPN 435
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 432 KVGEICVRGP---QVMKGYWNRPEETARAIMAD--GFFRTGDMGFMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVAAE 506
Cdd:cd05967 436 ELGNIVIKLPlppGCLLTLWKNDERFKKLYLSKfpGYYDTGDAGYKDEDGYLFIMGRTDDVINVAGHRLSTGEMEESVLS 515
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 507 HPGVLESAAVGIPNEHSGEVVKLYVV-----RRDPELTEDEVKAFCAERLTNYKRPREVEFRESLPKSNVGKILRRELRD 581
Cdd:cd05967 516 HPAVAECAVVGVRDELKGQVPLGLVVlkegvKITAEELEKELVALVREQIGPVAAFRLVIFVKRLPKTRSGKILRRTLRK 595
|
|
| A_NRPS_TlmIV_like |
cd12114 |
The adenylation domain of nonribosomal peptide synthetases (NRPS), including ... |
65-579 |
4.37e-41 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Streptoalloteichus tallysomycin biosynthesis genes; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the TLM biosynthetic gene cluster from Streptoalloteichus that consists of nine NRPS genes; the N-terminal module of TlmVI (NRPS-5) and the starter module of BlmVI (NRPS-5) are comprised of the acyl CoA ligase (AL) and acyl carrier protein (ACP)-like domains, which are thought to be involved in the biosynthesis of the beta-aminoalaninamide moiety.
Pssm-ID: 341279 [Multi-domain] Cd Length: 477 Bit Score: 155.12 E-value: 4.37e-41
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 65 NSPAFTCMGKDISYKELDDHSRALAAWLQSRGLVKGDRVAIMMP-NILQYPITFtAVLRAGFVVVNVNPLYTPRELEHQL 143
Cdd:cd12114 2 DATAVICGDGTLTYGELAERARRVAGALKAAGVRPGDLVAVTLPkGPEQVVAVL-GILAAGAAYVPVDIDQPAARREAIL 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 144 NDAGAKALVVlenfattvEKALPSINVPNILVAsmgdmlgfkghivnlvvrrvkkmvpawnipghvrFKDALAQGRAKSF 223
Cdd:cd12114 81 ADAGARLVLT--------DGPDAQLDVAVFDVL----------------------------------ILDLDALAAPAPP 118
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 224 NPVPVQGCDIAFLQYTGGTTGISKGAMLTHSNILANVEQMN---------LWMDVAfrnkgkpkALNFvcALPLYHIFAL 294
Cdd:cd12114 119 PPVDVAPDDLAYVIFTSGSTGTPKGVMISHRAALNTILDINrrfavgpddRVLALS--------SLSF--DLSVYDIFGA 188
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 295 TVnamigmkLGArNILIPNP---RDIPSFVKELKKYPVHIFPGLNTLFNGLMNNPDFQTLDFKPLILTLGGG--MAVQRP 369
Cdd:cd12114 189 LS-------AGA-TLVLPDEarrRDPAHWAELIERHGVTLWNSVPALLEMLLDVLEAAQALLPSLRLVLLSGdwIPLDLP 260
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 370 vAERWQHMTGCHITEGYGLSETS------PVAcanalDATEFTGTI--GLPMPSTDVVIRDDDGSDLLLGKVGEICVRGP 441
Cdd:cd12114 261 -ARLRALAPDARLISLGGATEASiwsiyhPID-----EVPPDWRSIpyGRPLANQRYRVLDPRGRDCPDWVPGELWIGGR 334
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 442 QVMKGYWNRPEETARAIMADG----FFRTGDMGFMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVAAEHPGVLESAAVG 517
Cdd:cd12114 335 GVALGYLGDPELTAARFVTHPdgerLYRTGDLGRYRPDGTLEFLGRRDGQVKVRGYRIELGEIEAALQAHPGVARAVVVV 414
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|...
gi 479816261 518 IPNEHSGEVVKLYVVRRD-PELTEDEVKAFCAERLTNYKRPREVEFRESLPKSNVGKILRREL 579
Cdd:cd12114 415 LGDPGGKRLAAFVVPDNDgTPIAPDALRAFLAQTLPAYMIPSRVIALEALPLTANGKVDRAAL 477
|
|
| A_NRPS_VisG_like |
cd17651 |
similar to adenylation domain of virginiamycin S synthetase; This family of the adenylation (A) ... |
66-580 |
6.11e-41 |
|
similar to adenylation domain of virginiamycin S synthetase; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes virginiamycin S synthetase (VisG) in Streptomyces virginiae; VisG is involved in virginiamycin S (VS) biosynthesis as the provider of an L-pheGly molecule, a highly specific substrate for the last condensation step by VisF. This family also includes linear gramicidin synthetase B (LgrB) in Brevibacillus brevis. Substrate specificity analysis using residues of the substrate-binding pockets of all 16 adenylation domains has shown good agreement of the substrate amino acids predicted with the sequence of linear gramicidin. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341306 [Multi-domain] Cd Length: 491 Bit Score: 154.81 E-value: 6.11e-41
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 66 SPAFTCMGKDISYKELDDHSRALAAWLQSRGLVKGDRVAIMMPNILQYPITFTAVLRAGFVVVNVNPLYTPRELEHQLND 145
Cdd:cd17651 11 APALVAEGRRLTYAELDRRANRLAHRLRARGVGPGDLVALCARRSAELVVALLAILKAGAAYVPLDPAYPAERLAFMLAD 90
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 146 AGAkALVVLEN---FATTVEKALPsinvpnilvasmgdmlgfkghivnlvvrrvkkmvpawnipghVRFKDALAQGRAKS 222
Cdd:cd17651 91 AGP-VLVLTHPalaGELAVELVAV------------------------------------------TLLDQPGAAAGADA 127
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 223 FNPVPVQGCDIAFLQYTGGTTGISKGAMLTHSNiLANVEQmnlWMDVAFRNKGKPKALNFVcalPL------YHIF-ALT 295
Cdd:cd17651 128 EPDPALDADDLAYVIYTSGSTGRPKGVVMPHRS-LANLVA---WQARASSLGPGARTLQFA---GLgfdvsvQEIFsTLC 200
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 296 VnamigmklGARNILIPNP--RDIPSFVKELKKYPVHI----FPGLNTLFNgLMNNPDFQTLDFKPLILT---LGGGMAV 366
Cdd:cd17651 201 A--------GATLVLPPEEvrTDPPALAAWLDEQRISRvflpTVALRALAE-HGRPLGVRLAALRYLLTGgeqLVLTEDL 271
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 367 QRPVAerwqHMTGCHITEGYGLSETSpVACANALDATEF----TGTIGLPMPSTDVVIRDDDGSDLLLGKVGEICVRGPQ 442
Cdd:cd17651 272 REFCA----GLPGLRLHNHYGPTETH-VVTALSLPGDPAawpaPPPIGRPIDNTRVYVLDAALRPVPPGVPGELYIGGAG 346
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 443 VMKGYWNRPEETARAIMADGF------FRTGDMGFMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVAAEHPGVLESAAV 516
Cdd:cd17651 347 LARGYLNRPELTAERFVPDPFvpgarmYRTGDLARWLPDGELEFLGRADDQVKIRGFRIELGEIEAALARHPGVREAVVL 426
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 479816261 517 GIPN-EHSGEVVKLYVVRRDPELTEDEVKAFCAERLTNYKRPREVEFRESLPKSNVGKILRRELR 580
Cdd:cd17651 427 AREDrPGEKRLVAYVVGDPEAPVDAAELRAALATHLPEYMVPSAFVLLDALPLTPNGKLDRRALP 491
|
|
| AFD_YhfT-like |
cd17633 |
fatty acid-CoA ligase VraA; This family of acyl-CoA ligases includes Bacillus subtilis YhfT, ... |
382-576 |
1.06e-40 |
|
fatty acid-CoA ligase VraA; This family of acyl-CoA ligases includes Bacillus subtilis YhfT, as well as long-chain fatty acid-CoA ligase VraA, all of which are as yet to be characterized. These proteins belong to the adenylate-forming enzymes which catalyze an ATP-dependent two-step reaction to first activate a carboxylate substrate as an adenylate and then transfer the carboxylate to the pantetheine group of either coenzyme A or an acyl-carrier protein. The active site of the domain is located at the interface of a large N-terminal subdomain and a smaller C-terminal subdomain
Pssm-ID: 341288 [Multi-domain] Cd Length: 320 Bit Score: 150.25 E-value: 1.06e-40
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 382 ITEGYGLSETSPVAcANALDATEFTGTIGLPMPSTDVVIRDDDGsdlllGKVGEICVRGPQVMKGYWnrpeeTARAIMAD 461
Cdd:cd17633 139 LIEFYGTSELSFIT-YNFNQESRPPNSVGRPFPNVEIEIRNADG-----GEIGKIFVKSEMVFSGYV-----RGGFSNPD 207
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 462 GFFRTGDMGFMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVAAEHPGVLESAAVGIPNEHSGEVVKLYVVrrDPELTED 541
Cdd:cd17633 208 GWMSVGDIGYVDEEGYLYLVGRESDMIIIGGINIFPTEIESVLKAIPGIEEAIVVGIPDARFGEIAVALYS--GDKLTYK 285
|
170 180 190
....*....|....*....|....*....|....*
gi 479816261 542 EVKAFCAERLTNYKRPREVEFRESLPKSNVGKILR 576
Cdd:cd17633 286 QLKRFLKQKLSRYEIPKKIIFVDSLPYTSSGKIAR 320
|
|
| FADD10 |
cd17635 |
adenylate forming domain, fatty acid CoA ligase (FadD10); This family contains long chain ... |
232-576 |
5.13e-39 |
|
adenylate forming domain, fatty acid CoA ligase (FadD10); This family contains long chain fatty acid CoA ligases, including FadD10 which is involved in the synthesis of a virulence-related lipopeptide. FadD10 is a fatty acyl-AMP ligase (FAAL) that transfers fatty acids to an acyl carrier protein. Structures of FadD10 in apo- and complexed form with dodecanoyl-AMP, show a novel open conformation, facilitated by its unique inter-domain and intermolecular interactions, which is critical for the enzyme to carry out the acyl transfer onto the acyl carrier protein (Rv0100) rather than coenzyme A.
Pssm-ID: 341290 [Multi-domain] Cd Length: 340 Bit Score: 146.25 E-value: 5.13e-39
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 232 DIAFLQYTGGTTGISKGAMLTHSNILANVEqmNLWMDVAFRNKGKPKALnfvcALPLYHIFALTVNAMIGMKLGARNILI 311
Cdd:cd17635 2 DPLAVIFTSGTTGEPKAVLLANKTFFAVPD--ILQKEGLNWVVGDVTYL----PLPATHIGGLWWILTCLIHGGLCVTGG 75
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 312 PNpRDIPSFVKELKKYPVHIFPGLNTLFNGLMN-----NPDFQTLDFkpliLTLGGGMAVQRPVAE-RWQHMTgcHITEG 385
Cdd:cd17635 76 EN-TTYKSLFKILTTNAVTTTCLVPTLLSKLVSelksaNATVPSLRL----IGYGGSRAIAADVRFiEATGLT--NTAQV 148
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 386 YGLSETSPVACANALDATEFTGTIGLPMPSTDVVIRDDDGSDLLLGKVGEICVRGPQVMKGYWNRPEETArAIMADGFFR 465
Cdd:cd17635 149 YGLSETGTALCLPTDDDSIEINAVGRPYPGVDVYLAATDGIAGPSASFGTIWIKSPANMLGYWNNPERTA-EVLIDGWVN 227
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 466 TGDMGFMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVAAEHPGVLESAAVGIPNEHSGEVVKLYVVRRDpELTEDEVKA 545
Cdd:cd17635 228 TGDLGERREDGFLFITGRSSESINCGGVKIAPDEVERIAEGVSGVQECACYEISDEEFGELVGLAVVASA-ELDENAIRA 306
|
330 340 350
....*....|....*....|....*....|....
gi 479816261 546 F---CAERLTNYKRPREVEFRESLPKSNVGKILR 576
Cdd:cd17635 307 LkhtIRRELEPYARPSTIVIVTDIPRTQSGKVKR 340
|
|
| PRK06018 |
PRK06018 |
putative acyl-CoA synthetase; Provisional |
77-581 |
3.16e-38 |
|
putative acyl-CoA synthetase; Provisional
Pssm-ID: 235673 [Multi-domain] Cd Length: 542 Bit Score: 147.98 E-value: 3.16e-38
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 77 SYKELDDHSRALAAWLQSRGLVKGDRVAIMMPNILQYPITFTAVLRAGFVVVNVNPLYTPRELEHQLNDAGAKALVVLEN 156
Cdd:PRK06018 41 TYAQIHDRALKVSQALDRDGIKLGDRVATIAWNTWRHLEAWYGIMGIGAICHTVNPRLFPEQIAWIINHAEDRVVITDLT 120
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 157 FATTVEKALPSINvpnilvasmgdmlgfkgHIVNLVVRRVKKMVPAWNIPGHVRFKDALAQGRA----KSFNPVPVQGcd 232
Cdd:PRK06018 121 FVPILEKIADKLP-----------------SVERYVVLTDAAHMPQTTLKNAVAYEEWIAEADGdfawKTFDENTAAG-- 181
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 233 iafLQYTGGTTGISKGAMLTH-SNILANVEQMNlwmdvafrnkgkPKALNFVCA------LPLYHIFALTVnAMIGMKLG 305
Cdd:PRK06018 182 ---MCYTSGTTGDPKGVLYSHrSNVLHALMANN------------GDALGTSAAdtmlpvVPLFHANSWGI-AFSAPSMG 245
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 306 ArNILIPNPR-DIPSFVKELKKYPVHIFPGLNTLFNGLMNNPDFQTLDFKPLILTLGGGMAVQRPVAERWQHMtGCHITE 384
Cdd:PRK06018 246 T-KLVMPGAKlDGASVYELLDTEKVTFTAGVPTVWLMLLQYMEKEGLKLPHLKMVVCGGSAMPRSMIKAFEDM-GVEVRH 323
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 385 GYGLSETSPVACANAL---------DAT-EFTGTIGLPMPSTDVVIRDDDGSDLLL-GKV-GEICVRGPQVMKGYWnrpE 452
Cdd:PRK06018 324 AWGMTEMSPLGTLAALkppfsklpgDARlDVLQKQGYPPFGVEMKITDDAGKELPWdGKTfGRLKVRGPAVAAAYY---R 400
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 453 ETARAIMADGFFRTGDMGFMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVAAEHPGVLESAAVGIPNEHSGEVVKLYVV 532
Cdd:PRK06018 401 VDGEILDDDGFFDTGDVATIDAYGYMRITDRSKDVIKSGGEWISSIDLENLAVGHPKVAEAAVIGVYHPKWDERPLLIVQ 480
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|
gi 479816261 533 -RRDPELTEDEVKAFCAERLTNYKRPREVEFRESLPKSNVGKILRRELRD 581
Cdd:PRK06018 481 lKPGETATREEILKYMDGKIAKWWMPDDVAFVDAIPHTATGKILKTALRE 530
|
|
| PLN02479 |
PLN02479 |
acetate-CoA ligase |
86-581 |
7.42e-38 |
|
acetate-CoA ligase
Pssm-ID: 178097 [Multi-domain] Cd Length: 567 Bit Score: 147.30 E-value: 7.42e-38
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 86 RALAAWLQSRGLVKGDRVAIMMPNIlqyPITFTA---VLRAGFVVVNVNPLYTPRELEHQLNDAGAKALVVLENFATTVE 162
Cdd:PLN02479 56 RRLASALAKRSIGPGSTVAVIAPNI---PAMYEAhfgVPMAGAVVNCVNIRLNAPTIAFLLEHSKSEVVMVDQEFFTLAE 132
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 163 KALPSIN-------VPNILVASMGDMLGFKGhiVNLVVRRvkkmvpawnipGHVRFKDALAQGRAK-SFNPVPVQGCDIA 234
Cdd:PLN02479 133 EALKILAekkkssfKPPLLIVIGDPTCDPKS--LQYALGK-----------GAIEYEKFLETGDPEfAWKPPADEWQSIA 199
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 235 fLQYTGGTTGISKGAMLTHSNI-LANVEQMNLWmdvafrnkGKPKALNFVCALPLYHI----FALTVNAMIGMKLGARNI 309
Cdd:PLN02479 200 -LGYTSGTTASPKGVVLHHRGAyLMALSNALIW--------GMNEGAVYLWTLPMFHCngwcFTWTLAALCGTNICLRQV 270
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 310 lipNPRDIPSFVKelkKYPVHIFPGLNTLFNGLMNNPDFQTLDFKPLILTLGGGMAVQRPVAERWQHMTGCHITEGYGLS 389
Cdd:PLN02479 271 ---TAKAIYSAIA---NYGVTHFCAAPVVLNTIVNAPKSETILPLPRVVHVMTAGAAPPPSVLFAMSEKGFRVTHTYGLS 344
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 390 ET---SPVaCA----------------NALDATEFTGTIGLpmpstDVVIRDD------DGSDLllgkvGEICVRGPQVM 444
Cdd:PLN02479 345 ETygpSTV-CAwkpewdslppeeqarlNARQGVRYIGLEGL-----DVVDTKTmkpvpaDGKTM-----GEIVMRGNMVM 413
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 445 KGYWNRPEETARAiMADGFFRTGDMGFMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVAAEHPGVLESAAVGIPNEHSG 524
Cdd:PLN02479 414 KGYLKNPKANEEA-FANGWFHSGDLGVKHPDGYIEIKDRSKDIIISGGENISSLEVENVVYTHPAVLEASVVARPDERWG 492
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|...
gi 479816261 525 E------VVKLYVVRRDPELTEDEVKAFCAERLTNYKRPREVEFrESLPKSNVGKILRRELRD 581
Cdd:PLN02479 493 EspcafvTLKPGVDKSDEAALAEDIMKFCRERLPAYWVPKSVVF-GPLPKTATGKIQKHVLRA 554
|
|
| A_NRPS_Bac |
cd17655 |
bacitracin synthetase and related proteins; This family of the adenylation (A) domain of ... |
65-579 |
2.16e-37 |
|
bacitracin synthetase and related proteins; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes bacitracin synthetases 1, 2, and 3 (BA1, also known as ATP-dependent cysteine adenylase or cysteine activase, BA2, also known as ATP-dependent lysine adenylase or lysine activase, and BA3, also known as ATP-dependent isoleucine adenylase or isoleucine activase) in Bacilli. Bacitracin is a mixture of related cyclic peptides used as a polypeptide antibiotic. This family also includes gramicidin synthetase 1 involved in synthesis of the cyclic peptide antibiotic gramicidin S via activation of phenylalanine. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341310 [Multi-domain] Cd Length: 490 Bit Score: 144.78 E-value: 2.16e-37
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 65 NSPAFTCMGKDISYKELDDHSRALAAWLQSRGLVKGDRVAIMMPNILQYPITFTAVLRAGFVVVNVNPLYTPRELEHQLN 144
Cdd:cd17655 12 DHTAVVFEDQTLTYRELNERANQLARTLREKGVGPDTIVGIMAERSLEMIVGILGILKAGGAYLPIDPDYPEERIQYILE 91
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 145 DAGAKALVVlenfattvEKALPsinvPNILvasmgdmlgFKGHIVNLVvrrvkkmvpawnipghvrfKDALAQGRAKSFN 224
Cdd:cd17655 92 DSGADILLT--------QSHLQ----PPIA---------FIGLIDLLD-------------------EDTIYHEESENLE 131
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 225 PvPVQGCDIAFLQYTGGTTGISKGAMLTHSNILANVEqmnlwmdvAFRNKG-KPKALNFVCALPLYhiFALTVNAMI-GM 302
Cdd:cd17655 132 P-VSKSDDLAYVIYTSGSTGKPKGVMIEHRGVVNLVE--------WANKVIyQGEHLRVALFASIS--FDASVTEIFaSL 200
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 303 KLGARNILIPNPR--DIPSFVKELKKYPVHIFPGLNTLFNGLMNNPDFQTLDFKPLILtlgGGMAVQRPVAERWQHM--T 378
Cdd:cd17655 201 LSGNTLYIVRKETvlDGQALTQYIRQNRITIIDLTPAHLKLLDAADDSEGLSLKHLIV---GGEALSTELAKKIIELfgT 277
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 379 GCHITEGYGLSETspVACANALDATEFTGT-----IGLPMPSTDVVIRDDDGSDLLLGKVGEICVRGPQVMKGYWNRPEE 453
Cdd:cd17655 278 NPTITNAYGPTET--TVDASIYQYEPETDQqvsvpIGKPLGNTRIYILDQYGRPQPVGVAGELYIGGEGVARGYLNRPEL 355
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 454 TARAIMADGF------FRTGDMGFMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVAAEHPGVLESAAVGIPNEHSGEVV 527
Cdd:cd17655 356 TAEKFVDDPFvpgermYRTGDLARWLPDGNIEFLGRIDHQVKIRGYRIELGEIEARLLQHPDIKEAVVIARKDEQGQNYL 435
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|..
gi 479816261 528 KLYVVrRDPELTEDEVKAFCAERLTNYKRPREVEFRESLPKSNVGKILRREL 579
Cdd:cd17655 436 CAYIV-SEKELPVAQLREFLARELPDYMIPSYFIKLDEIPLTPNGKVDRKAL 486
|
|
| A_NRPS_ProA |
cd17656 |
gramicidin S synthase 2, also known as ATP-dependent proline adenylase; This family of the ... |
76-579 |
6.72e-36 |
|
gramicidin S synthase 2, also known as ATP-dependent proline adenylase; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) contains gramicidin S synthase 2 (also known as ATP-dependent proline adenylase or proline activase or ProA). ProA is a multifunctional enzyme involved in synthesis of the cyclic peptide antibiotic gramicidin S and able to activate and polymerize the amino acids proline, valine, ornithine and leucine. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341311 [Multi-domain] Cd Length: 479 Bit Score: 140.30 E-value: 6.72e-36
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 76 ISYKELDDHSRALAAWLQSRGLVKGDRVAIMMPNILQYPITFTAVLRAGFVVVNVNPLYTPRELEHQLNDAGAKALVVle 155
Cdd:cd17656 14 LTYRELNERSNQLARFLREKGVKKDSIVAIMMERSAEMIVGILGILKAGGAFVPIDPEYPEERRIYIMLDSGVRVVLT-- 91
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 156 nfattvekalpsinvpnilVASMGDMLGFKGHIVnlvvrrvkkmVPAWnipghvrfkDALAQGRAKSFNPVpVQGCDIAF 235
Cdd:cd17656 92 -------------------QRHLKSKLSFNKSTI----------LLED---------PSISQEDTSNIDYI-NNSDDLLY 132
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 236 LQYTGGTTGISKGAMLTHSNILANVEQMNLWMDVAFRNKgkpkALNF------VCalplYH-IFALTVNamigmklGARN 308
Cdd:cd17656 133 IIYTSGTTGKPKGVQLEHKNMVNLLHFEREKTNINFSDK----VLQFatcsfdVC----YQeIFSTLLS-------GGTL 197
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 309 ILIPNP--RDIPSFVKELKKYPVHI--FPG--LNTLFNGLMNNPDFQTlDFKPLIlTLGGGMAVQRPVAErWQHMTGCHI 382
Cdd:cd17656 198 YIIREEtkRDVEQLFDLVKRHNIEVvfLPVafLKFIFSEREFINRFPT-CVKHII-TAGEQLVITNEFKE-MLHEHNVHL 274
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 383 TEGYGLSETSPV-ACA-NALDATEFTGTIGLPMPSTDVVIRDDDGSDLLLGKVGEICVRGPQVMKGYWNRPEETARAIMA 460
Cdd:cd17656 275 HNHYGPSETHVVtTYTiNPEAEIPELPPIGKPISNTWIYILDQEQQLQPQGIVGELYISGASVARGYLNRQELTAEKFFP 354
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 461 DGF------FRTGDMGFMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVAAEHPGVLESAAVGIPNEHSGEVVKLYVVrR 534
Cdd:cd17656 355 DPFdpnermYRTGDLARYLPDGNIEFLGRADHQVKIRGYRIELGEIEAQLLNHPGVSEAVVLDKADDKGEKYLCAYFV-M 433
|
490 500 510 520
....*....|....*....|....*....|....*....|....*
gi 479816261 535 DPELTEDEVKAFCAERLTNYKRPREVEFRESLPKSNVGKILRREL 579
Cdd:cd17656 434 EQELNISQLREYLAKQLPEYMIPSFFVPLDQLPLTPNGKVDRKAL 478
|
|
| A_NRPS_SidN3_like |
cd05918 |
The adenylation (A) domain of siderophore-synthesizing nonribosomal peptide synthetases (NRPS); ... |
52-581 |
2.04e-35 |
|
The adenylation (A) domain of siderophore-synthesizing nonribosomal peptide synthetases (NRPS); The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. This family of siderophore-synthesizing NRPS includes the third adenylation domain of SidN from the endophytic fungus Neotyphodium lolii, ferrichrome siderophore synthetase, HC-toxin synthetase, and enniatin synthase. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341242 [Multi-domain] Cd Length: 481 Bit Score: 139.21 E-value: 2.04e-35
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 52 ISDMISASCRQFANSPAFTCMGKDISYKELDDHSRALAAWLQSRGLVKGDRVAIMMPNILQYPITFTAVLRAGFVVVNVN 131
Cdd:cd05918 1 VHDLIEERARSQPDAPAVCAWDGSLTYAELDRLSSRLAHHLRSLGVGPGVFVPLCFEKSKWAVVAMLAVLKAGGAFVPLD 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 132 PLYtPRE-LEHQLNDAGAKalvvlenfattvekalpsinvpnILVASMGDmlgfkghivnlvvrrvkkmvpawnipghvr 210
Cdd:cd05918 81 PSH-PLQrLQEILQDTGAK-----------------------VVLTSSPS------------------------------ 106
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 211 fkdalaqgraksfnpvpvqgcDIAFLQYTGGTTGISKGAMLTHSNILANVEQMNLwmdvAFRNKGKPKALNF------VC 284
Cdd:cd05918 107 ---------------------DAAYVIFTSGSTGKPKGVVIEHRALSTSALAHGR----ALGLTSESRVLQFasytfdVS 161
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 285 alpLYHIF-ALTVNAMIgmklgarniLIPNPR----DIPSFVKELKkyPVHIF--PglnTLFNgLMNNPDFQTLdfKPLI 357
Cdd:cd05918 162 ---ILEIFtTLAAGGCL---------CIPSEEdrlnDLAGFINRLR--VTWAFltP---SVAR-LLDPEDVPSL--RTLV 221
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 358 LtlgGGMAVQRPVAERWQHmtGCHITEGYGLSETSPVACANALDATEFTGTIGLPMPSTD-VVIRDDDGSDLLLGKVGEI 436
Cdd:cd05918 222 L---GGEALTQSDVDTWAD--RVRLINAYGPAECTIAATVSPVVPSTDPRNIGRPLGATCwVVDPDNHDRLVPIGAVGEL 296
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 437 CVRGPQVMKGYWNRPEETARA-------IMADG------FFRTGDMGFMDERGYTKIVDRKKDMILVSGFNVYPNEIEEV 503
Cdd:cd05918 297 LIEGPILARGYLNDPEKTAAAfiedpawLKQEGsgrgrrLYRTGDLVRYNPDGSLEYVGRKDTQVKIRGQRVELGEIEHH 376
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 504 AAEHPGVLESAAVGIPNEHSGEVVKLYV--VRRDPELTED-------------------EVKAFCAERLTNYKRPREVEF 562
Cdd:cd05918 377 LRQSLPGAKEVVVEVVKPKDGSSSPQLVafVVLDGSSSGSgdgdslflepsdefralvaELRSKLRQRLPSYMVPSVFLP 456
|
570
....*....|....*....
gi 479816261 563 RESLPKSNVGKILRRELRD 581
Cdd:cd05918 457 LSHLPLTASGKIDRRALRE 475
|
|
| LC_FACS_euk1 |
cd17639 |
Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS), including fungal proteins; The ... |
232-488 |
1.26e-34 |
|
Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS), including fungal proteins; The members of this family are eukaryotic fatty acid CoA synthetases (EC 6.2.1.3) that activate fatty acids with chain lengths of 12 to 20 and includes fungal proteins. They act on a wide range of long-chain saturated and unsaturated fatty acids, but the enzymes from different tissues show some variation in specificity. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Organisms tend to have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells. In Schizosaccharomyces pombe, lcf1 gene encodes a new fatty acyl-CoA synthetase that preferentially recognizes myristic acid as a substrate.
Pssm-ID: 341294 [Multi-domain] Cd Length: 507 Bit Score: 137.35 E-value: 1.26e-34
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 232 DIAFLQYTGGTTGISKGAMLTHSNILANVEQMNLWMDVAFRNKgkpkalNFVCA-LPLYHIFALTVNaMIGMKLGARnIL 310
Cdd:cd17639 89 DLACIMYTSGSTGNPKGVMLTHGNLVAGIAGLGDRVPELLGPD------DRYLAyLPLAHIFELAAE-NVCLYRGGT-IG 160
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 311 IPNPR------------DIPSF--------------VKE--LKKypVHIFPGL-NTLFNGLMN-----------NPDFQT 350
Cdd:cd17639 161 YGSPRtltdkskrgckgDLTEFkptlmvgvpaiwdtIRKgvLAK--LNPMGGLkRTLFWTAYQsklkalkegpgTPLLDE 238
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 351 LDFKPLILTLGG-------GMAVQRPVAERWQHMTGCHITEGYGLSETspVACANALDATEF-TGTIGLPMPSTDVVIRD 422
Cdd:cd17639 239 LVFKKVRAALGGrlrymlsGGAPLSADTQEFLNIVLCPVIQGYGLTET--CAGGTVQDPGDLeTGRVGPPLPCCEIKLVD 316
|
250 260 270 280 290 300 310
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 479816261 423 -DDGsdlllGKV-------GEICVRGPQVMKGYWNRPEETARAIMADGFFRTGDMGFMDERGYTKIVDRKKDMI 488
Cdd:cd17639 317 wEEG-----GYStdkppprGEILIRGPNVFKGYYKNPEKTKEAFDGDGWFHTGDIGEFHPDGTLKIIDRKKDLV 385
|
|
| PLN02860 |
PLN02860 |
o-succinylbenzoate-CoA ligase |
79-580 |
1.42e-34 |
|
o-succinylbenzoate-CoA ligase
Pssm-ID: 215464 [Multi-domain] Cd Length: 563 Bit Score: 138.01 E-value: 1.42e-34
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 79 KELDDHSRALAAWLQSRGLVKGDRVAIMMPNILQYPITFTAVLRAGFVVVNVNPLYTPRELEHQLNDAGAKALVVLENFA 158
Cdd:PLN02860 36 HEFVDGVLSLAAGLLRLGLRNGDVVAIAALNSDLYLEWLLAVACAGGIVAPLNYRWSFEEAKSAMLLVRPVMLVTDETCS 115
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 159 TTVEK----ALPSINvpniLVASMGDMLGFKGHIVNLVVRrvkkmvpawniPGHVRfKDALAQgraKSFNPVPVQGcDIA 234
Cdd:PLN02860 116 SWYEElqndRLPSLM----WQVFLESPSSSVFIFLNSFLT-----------TEMLK-QRALGT---TELDYAWAPD-DAV 175
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 235 FLQYTGGTTGISKGAMLTHSNI----LANV-----EQMNLWMDVAfrnkgkpkalnfvcalPLYHIFALTvNAMIGMKLG 305
Cdd:PLN02860 176 LICFTSGTTGRPKGVTISHSALivqsLAKIaivgyGEDDVYLHTA----------------PLCHIGGLS-SALAMLMVG 238
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 306 ARNILIPNpRDIPSFVKELKKYPVHIFPGLNTLFNGLMN-NPDFQTLDFKPLILTL--GGGMAVQRPVAERWQHMTGCHI 382
Cdd:PLN02860 239 ACHVLLPK-FDAKAALQAIKQHNVTSMITVPAMMADLISlTRKSMTWKVFPSVRKIlnGGGSLSSRLLPDAKKLFPNAKL 317
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 383 TEGYGLSET----------SPVACANALDATEFTGT------------IGLPMPSTDVVIRDDDGSdlllgKVGEICVRG 440
Cdd:PLN02860 318 FSAYGMTEAcssltfmtlhDPTLESPKQTLQTVNQTksssvhqpqgvcVGKPAPHVELKIGLDESS-----RVGRILTRG 392
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 441 PQVMKGYWNRPEETARAIMADGFFRTGDMGFMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVAAEHPGVLESAAVGIPN 520
Cdd:PLN02860 393 PHVMLGYWGQNSETASVLSNDGWLDTGDIGWIDKAGNLWLIGRSNDRIKTGGENVYPEEVEAVLSQHPGVASVVVVGVPD 472
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 479816261 521 EHSGEVVKLYVVRRDP---------------ELTEDEVKAFCAER-LTNYKRPRE-VEFRESLPKSNVGKILRRELR 580
Cdd:PLN02860 473 SRLTEMVVACVRLRDGwiwsdnekenakknlTLSSETLRHHCREKnLSRFKIPKLfVQWRKPFPLTTTGKIRRDEVR 549
|
|
| MACS_like_1 |
cd05974 |
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the ... |
76-580 |
2.62e-34 |
|
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. MACS enzymes are localized to mitochondria.
Pssm-ID: 341278 [Multi-domain] Cd Length: 432 Bit Score: 135.00 E-value: 2.62e-34
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 76 ISYKELDDHSRALAAWLQSRGLVKGDRVAIMMPNILQYPITFTAVLRAGFVVVNVNPLYTPRELEHQLNDAGAKALVVLE 155
Cdd:cd05974 1 VSFAEMSARSSRVANFLRSIGVGRGDRILLMLGNVVELWEAMLAAMKLGAVVIPATTLLTPDDLRDRVDRGGAVYAAVDE 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 156 NFATTvekalpsinvpnilvasmgdmlgfkghivnlvvrrvkkmvpawnipghvrfkdalaqgraksfnpvpvqgcDIAF 235
Cdd:cd05974 81 NTHAD-----------------------------------------------------------------------DPML 89
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 236 LQYTGGTTGISKGAMLTHSNILANVEQMNLWMDVafrnkgKPKALNFVCALPLYHIFALTvNAMIGMKLGARNILIPNPR 315
Cdd:cd05974 90 LYFTSGTTSKPKLVEHTHRSYPVGHLSTMYWIGL------KPGDVHWNISSPGWAKHAWS-CFFAPWNAGATVFLFNYAR 162
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 316 -DIPSFVKELKKYPVHIFPGLNTLFNGLMNNpDFQTLDFkPLILTLGGGMAVQRPVAERWQHMTGCHITEGYGLSETSpV 394
Cdd:cd05974 163 fDAKRVLAALVRYGVTTLCAPPTVWRMLIQQ-DLASFDV-KLREVVGAGEPLNPEVIEQVRRAWGLTIRDGYGQTETT-A 239
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 395 ACANALDATEFTGTIGLPMPSTDVVIRDDDGSDlllGKVGEICV-----RGPQVMKGYWNRPEETARAiMADGFFRTGDM 469
Cdd:cd05974 240 LVGNSPGQPVKAGSMGRPLPGYRVALLDPDGAP---ATEGEVALdlgdtRPVGLMKGYAGDPDKTAHA-MRGGYYRTGDI 315
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 470 GFMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVAAEHPGVLESAAVGIPNEHSGEVVKLYVV-----RRDPELTEDEVK 544
Cdd:cd05974 316 AMRDEDGYLTYVGRADDVFKSSDYRISPFELESVLIEHPAVAEAAVVPSPDPVRLSVPKAFIVlragyEPSPETALEIFR 395
|
490 500 510
....*....|....*....|....*....|....*.
gi 479816261 545 aFCAERLTNYKRPREVEFREsLPKSNVGKILRRELR 580
Cdd:cd05974 396 -FSRERLAPYKRIRRLEFAE-LPKTISGKIRRVELR 429
|
|
| A_NRPS_Sfm_like |
cd12115 |
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Saframycin A gene ... |
52-579 |
9.46e-34 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Saframycin A gene cluster from Streptomyces lavendulae; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the saframycin A gene cluster from Streptomyces lavendulae which implicates the NRPS system for assembling the unusual tetrapeptidyl skeleton in an iterative manner. It also includes saframycin Mx1 produced by Myxococcus xanthus NRPS.
Pssm-ID: 341280 [Multi-domain] Cd Length: 447 Bit Score: 133.60 E-value: 9.46e-34
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 52 ISDMISASCRQFANSPAFTCMGKDISYKELDDHSRALAAWLQSRGLVKGDRVAIMMPNILQYPITFTAVLRAGFVVVNVN 131
Cdd:cd12115 1 LHDLVEAQAARTPDAIALVCGDESLTYAELNRRANRLAARLRAAGVGPESRVGVCLERTPDLVVALLAVLKAGAAYVPLD 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 132 PLYTPRELEHQLNDAGAKALVVlenfattvekalpsinvpnilvasmgdmlgfkghivnlvvrrvkkmvpawnipghvrf 211
Cdd:cd12115 81 PAYPPERLRFILEDAQARLVLT---------------------------------------------------------- 102
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 212 kdalaqgraksfnpvpvQGCDIAFLQYTGGTTGISKGAMLTHSNILANVEqmnlWMDVAFrnkgKPKALNFVCA------ 285
Cdd:cd12115 103 -----------------DPDDLAYVIYTSGSTGRPKGVAIEHRNAAAFLQ----WAAAAF----SAEELAGVLAstsicf 157
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 286 -LPLYHIFA-LTVNAMIGMklgARNI--LIPNPR--------DIPSFVKELKKYPVhIFPGLNTLfnGLMNNPDFQTLdf 353
Cdd:cd12115 158 dLSVFELFGpLATGGKVVL---ADNVlaLPDLPAaaevtlinTVPSAAAELLRHDA-LPASVRVV--NLAGEPLPRDL-- 229
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 354 kpliltlgggmavqrpVAERWQHMTGCHITEGYGLSETSPVACANALDA-TEFTGTIGLPMPSTDVVIRDDDGSDLLLGK 432
Cdd:cd12115 230 ----------------VQRLYARLQVERVVNLYGPSEDTTYSTVAPVPPgASGEVSIGRPLANTQAYVLDRALQPVPLGV 293
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 433 VGEICVRGPQVMKGYWNRPEETARAIMADGF------FRTGDMGFMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVAAE 506
Cdd:cd12115 294 PGELYIGGAGVARGYLGRPGLTAERFLPDPFgpgarlYRTGDLVRWRPDGLLEFLGRADNQVKVRGFRIELGEIEAALRS 373
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 479816261 507 HPGVLESAAVGIPNEHSGEVVKLYVVRRDPE-LTEDEVKAFCAERLTNYKRPREVEFRESLPKSNVGKILRREL 579
Cdd:cd12115 374 IPGVREAVVVAIGDAAGERRLVAYIVAEPGAaGLVEDLRRHLGTRLPAYMVPSRFVRLDALPLTPNGKIDRSAL 447
|
|
| PRK09192 |
PRK09192 |
fatty acyl-AMP ligase; |
78-580 |
2.64e-33 |
|
fatty acyl-AMP ligase;
Pssm-ID: 236403 [Multi-domain] Cd Length: 579 Bit Score: 134.36 E-value: 2.64e-33
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 78 YKELDDHSRALAAWLQSRGLVKGDRVAIMMPNILQYPITFTAVLRAGFVVVnvnPLYTP-----RE-----LEHQLNDAG 147
Cdd:PRK09192 52 YQTLRARAEAGARRLLALGLKPGDRVALIAETDGDFVEAFFACQYAGLVPV---PLPLPmgfggREsyiaqLRGMLASAQ 128
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 148 AKALVVLENFATTVEKALPSinvpnilvasmgdmlgfkghivnlvvrrvkkmvpawnipghvrfKDALAQGRAKSFNPVP 227
Cdd:PRK09192 129 PAAIITPDELLPWVNEATHG--------------------------------------------NPLLHVLSHAWFKALP 164
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 228 VQGC--------DIAFLQYTGGTTGISKGAMLTHSNILANVeqmnlwmdVAFRNKGkpkaLNFV----CA--LPLYHI-- 291
Cdd:PRK09192 165 EADValprptpdDIAYLQYSSGSTRFPRGVIITHRALMANL--------RAISHDG----LKVRpgdrCVswLPFYHDmg 232
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 292 ---FALTVNA----------------------MI-------------GMKLGARNIlipNPRDIPSFvkELKKYPVhifP 333
Cdd:PRK09192 233 lvgFLLTPVAtqlsvdylptrdfarrplqwldLIsrnrgtisysppfGYELCARRV---NSKDLAEL--DLSCWRV---A 304
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 334 GLNtlfnGLMNNPD--------FQTLDFKP-------------LILT---LGGGMAVQRPVAERwqhmtgchiTEGYGLS 389
Cdd:PRK09192 305 GIG----ADMIRPDvlhqfaeaFAPAGFDDkafmpsyglaeatLAVSfspLGSGIVVEEVDRDR---------LEYQGKA 371
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 390 ETSPvacANALDATEFTgTIGLPMPSTDVVIRDDDGSDLLLGKVGEICVRGPQVMKGYWNRpEETARAIMADGFFRTGDM 469
Cdd:PRK09192 372 VAPG---AETRRVRTFV-NCGKALPGHEIEIRNEAGMPLPERVVGHICVRGPSLMSGYFRD-EESQDVLAADGWLDTGDL 446
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 470 GFMDErGYTKIVDRKKDMILVSGFNVYPNEIEEVAAEHPGVL--ESAAVGIPNEHSGEVVKLYVVRrdpeLTEDEVKAFC 547
Cdd:PRK09192 447 GYLLD-GYLYITGRAKDLIIINGRNIWPQDIEWIAEQEPELRsgDAAAFSIAQENGEKIVLLVQCR----ISDEERRGQL 521
|
570 580 590 600
....*....|....*....|....*....|....*....|
gi 479816261 548 AERLTNYKRPR-----EVEF--RESLPKSNVGKILRRELR 580
Cdd:PRK09192 522 IHALAALVRSEfgveaAVELvpPHSLPRTSSGKLSRAKAK 561
|
|
| PLN03102 |
PLN03102 |
acyl-activating enzyme; Provisional |
88-581 |
3.14e-33 |
|
acyl-activating enzyme; Provisional
Pssm-ID: 215576 [Multi-domain] Cd Length: 579 Bit Score: 133.99 E-value: 3.14e-33
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 88 LAAWLQSRGLVKGDRVAIMMPNI-LQYPITFtAVLRAGFVVVNVNPLYTPRELEHQLNDAGAKALVVLENFATTVEKALP 166
Cdd:PLN03102 52 LAASLISLNITKNDVVSVLAPNTpAMYEMHF-AVPMAGAVLNPINTRLDATSIAAILRHAKPKILFVDRSFEPLAREVLH 130
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 167 sinvpniLVASMGDMLGFKGHIVNLVvrrvkkmvpawNIPGHVRFKDALAQGRAKSFNPVPVQGC---------DIAFLQ 237
Cdd:PLN03102 131 -------LLSSEDSNLNLPVIFIHEI-----------DFPKRPSSEELDYECLIQRGEPTPSLVArmfriqdehDPISLN 192
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 238 YTGGTTGISKGAMLTHSNI-LANVEQMNLWMDVAFrnkgkPKalnFVCALPLYH----IFALTVNAMIGMKLGARNIlip 312
Cdd:PLN03102 193 YTSGTTADPKGVVISHRGAyLSTLSAIIGWEMGTC-----PV---YLWTLPMFHcngwTFTWGTAARGGTSVCMRHV--- 261
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 313 nprDIPSFVKELKKYPVHIFPGLNTLFNGLMNNPDFQTLDFKPLILTLGGGMAVQRPVAERWQHMtGCHITEGYGLSE-T 391
Cdd:PLN03102 262 ---TAPEIYKNIEMHNVTHMCCVPTVFNILLKGNSLDLSPRSGPVHVLTGGSPPPAALVKKVQRL-GFQVMHAYGLTEaT 337
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 392 SPVA-CA-----------NALDATEFTGTIGLPMPSTDVVIRDDDGSDLLLGK-VGEICVRGPQVMKGYWNRPEETARAi 458
Cdd:PLN03102 338 GPVLfCEwqdewnrlpenQQMELKARQGVSILGLADVDVKNKETQESVPRDGKtMGEIVIKGSSIMKGYLKNPKATSEA- 416
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 459 MADGFFRTGDMGFMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVAAEHPGVLESAAVGIPNEHSGEVVKLYVVRRDPEL 538
Cdd:PLN03102 417 FKHGWLNTGDVGVIHPDGHVEIKDRSKDIIISGGENISSVEVENVLYKYPKVLETAVVAMPHPTWGETPCAFVVLEKGET 496
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....
gi 479816261 539 T-----------EDEVKAFCAERLTNYKRPREVEFRESLPKSNVGKILRRELRD 581
Cdd:PLN03102 497 TkedrvdklvtrERDLIEYCRENLPHFMCPRKVVFLQELPKNGNGKILKPKLRD 550
|
|
| PRK12316 |
PRK12316 |
peptide synthase; Provisional |
43-579 |
4.62e-33 |
|
peptide synthase; Provisional
Pssm-ID: 237054 [Multi-domain] Cd Length: 5163 Bit Score: 135.86 E-value: 4.62e-33
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 43 DINLSAATSISDMISASCRQFANSPAFTCMGKDISYKELDDHSRALAAWLQSRGLVKGDRVAIMMPNILQYPITFTAVLR 122
Cdd:PRK12316 4544 DAGYPATRCVHQLVAERARMTPDAVAVVFDEEKLTYAELNRRANRLAHALIARGVGPEVLVGIAMERSAEMMVGLLAVLK 4623
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 123 AGFVVVNVNPLYtPRE-LEHQLNDAGAKALVvlenfatTVEKALPSINVPNILVAsmgdmlgfkghivnLVVRRVKKmvp 201
Cdd:PRK12316 4624 AGGAYVPLDPEY-PRErLAYMMEDSGAALLL-------TQSHLLQRLPIPDGLAS--------------LALDRDED--- 4678
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 202 aWnipghvrfkdalaQGRAKSFNPVPVQGCDIAFLQYTGGTTGISKGAMLTHSNILANVeqmnLWMDVAFRNKGKPKALN 281
Cdd:PRK12316 4679 -W-------------EGFPAHDPAVRLHPDNLAYVIYTSGSTGRPKGVAVSHGSLVNHL----HATGERYELTPDDRVLQ 4740
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 282 FVCalplyHIFALTV-NAMIGMKLGARnILIPNPR--DIPSFVKELKKYPVHIFPGLNTLFNGLMNNPDFQTlDFKPLIL 358
Cdd:PRK12316 4741 FMS-----FSFDGSHeGLYHPLINGAS-VVIRDDSlwDPERLYAEIHEHRVTVLVFPPVYLQQLAEHAERDG-EPPSLRV 4813
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 359 TLGGGMAVQRPVAER-WQHMTGCHITEGYGLSETSPV----ACANALDATEFTGTIGLPMPSTDVVIRDDDGSDLLLGKV 433
Cdd:PRK12316 4814 YCFGGEAVAQASYDLaWRALKPVYLFNGYGPTETTVTvllwKARDGDACGAAYMPIGTPLGNRSGYVLDGQLNPLPVGVA 4893
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 434 GEICVRGPQVMKGYWNRPEETARAIMADGF-------FRTGDMGFMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVAAE 506
Cdd:PRK12316 4894 GELYLGGEGVARGYLERPALTAERFVPDPFgapggrlYRTGDLARYRADGVIDYLGRVDHQVKIRGFRIELGEIEARLRE 4973
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 507 HPGVLESAAVGIPNEHSGEVVKlYVVRRDPELTE---------DEVKAFCAERLTNYKRPREVEFRESLPKSNVGKILRR 577
Cdd:PRK12316 4974 HPAVREAVVIAQEGAVGKQLVG-YVVPQDPALADadeaqaelrDELKAALRERLPEYMVPAHLVFLARMPLTPNGKLDRK 5052
|
..
gi 479816261 578 EL 579
Cdd:PRK12316 5053 AL 5054
|
|
| PRK09274 |
PRK09274 |
peptide synthase; Provisional |
76-539 |
1.01e-32 |
|
peptide synthase; Provisional
Pssm-ID: 236443 [Multi-domain] Cd Length: 552 Bit Score: 132.33 E-value: 1.01e-32
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 76 ISYKELDDHSRALAAWLQSRGLVKGDRVAIMM-PNILQYPITFtAVLRAGFVVVNVNPLYTPRELEHQLNDAGAKALvvl 154
Cdd:PRK09274 42 LSFAELDARSDAIAHGLNAAGIGRGMRAVLMVtPSLEFFALTF-ALFKAGAVPVLVDPGMGIKNLKQCLAEAQPDAF--- 117
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 155 enfattvekalpsINVPnilvasmgdmlgfKGHIVNLVVRR----VKKMV---PAWnIPGHVRFKDALAQGRAKSFNPVP 227
Cdd:PRK09274 118 -------------IGIP-------------KAHLARRLFGWgkpsVRRLVtvgGRL-LWGGTTLATLLRDGAAAPFPMAD 170
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 228 VQGCDIAFLQYTGGTTGISKGAMLTHSNILANVEQM-NLW-MDVAFRNkgkpkalnfvcaLPLYHIFALtvnamIGMKLG 305
Cdd:PRK09274 171 LAPDDMAAILFTSGSTGTPKGVVYTHGMFEAQIEALrEDYgIEPGEID------------LPTFPLFAL-----FGPALG 233
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 306 ARNIlIP----------NPRDIpsfVKELKKYPV-HIF--PGL-NTLFN-GLMNNPDFQTLDfkpliLTLGGGMAVQRPV 370
Cdd:PRK09274 234 MTSV-IPdmdptrpatvDPAKL---FAAIERYGVtNLFgsPALlERLGRyGEANGIKLPSLR-----RVISAGAPVPIAV 304
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 371 AERWQHM--TGCHITEGYGLSETSPVACANA---LDAT-EFT----GT-IGLPMPSTDVVIRD---------DDGSDLLL 430
Cdd:PRK09274 305 IERFRAMlpPDAEILTPYGATEALPISSIESreiLFATrAATdngaGIcVGRPVDGVEVRIIAisdapipewDDALRLAT 384
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 431 GKVGEICVRGPQVMKGYWNRPEETARAIMADG----FFRTGDMGFMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVAAE 506
Cdd:PRK09274 385 GEIGEIVVAGPMVTRSYYNRPEATRLAKIPDGqgdvWHRMGDLGYLDAQGRLWFCGRKAHRVETAGGTLYTIPCERIFNT 464
|
490 500 510
....*....|....*....|....*....|...
gi 479816261 507 HPGVLESAAVGIPNehSGEVVKLYVVRRDPELT 539
Cdd:PRK09274 465 HPGVKRSALVGVGV--PGAQRPVLCVELEPGVA 495
|
|
| ttLC_FACS_like |
cd05915 |
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles; This family includes ... |
75-580 |
1.85e-32 |
|
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles; This family includes fatty acyl-CoA synthetases that can activate medium-chain to long-chain fatty acids. They catalyze the ATP-dependent acylation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. Fatty acyl-CoA synthetases are responsible for fatty acid degradation as well as physiological regulation of cellular functions via the production of fatty acyl-CoA esters. The fatty acyl-CoA synthetase from Thermus thermophiles in this family has been shown to catalyze the long-chain fatty acid, myristoyl acid, while another member in this family, the AlkK protein identified in Pseudomonas oleovorans, targets medium chain fatty acids. This family also includes an uncharacterized subgroup of FACS.
Pssm-ID: 213283 [Multi-domain] Cd Length: 509 Bit Score: 131.01 E-value: 1.85e-32
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 75 DISYK--ELDDHSRALAAWLQSRGLVKGDRVAIMMPNILQYPITFTAVLRAGFVVVNVNPLYTPRELEHQLNDAGAKALV 152
Cdd:cd05915 22 VHRTTyaEVYQRARRLMGGLRALGVGVGDRVATLGFNHFRHLEAYFAVPGMGAVLHTANPRLSPKEIAYILNHAEDKVLL 101
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 153 VLENFATTVEKALPSINvpnilvasmgdmlgfkgHIVNLVVRRVKkmvpawnipgHVRFKDALAQGRAkSFNPV-PVQGC 231
Cdd:cd05915 102 FDPNLLPLVEAIRGELK-----------------TVQHFVVMDEK----------APEGYLAYEEALG-EEADPvRVPER 153
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 232 DIAFLQYTGGTTGISKGAMLTHSNILANVEQMNLWMDVAfrnkGKPKALnFVCALPLYHI----FALTVNAMIGMKLGAR 307
Cdd:cd05915 154 AACGMAYTTGTTGLPKGVVYSHRALVLHSLAASLVDGTA----LSEKDV-VLPVVPMFHVnawcLPYAATLVGAKQVLPG 228
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 308 NIliPNPRDIpsfVKELKKYPVHIFPGLNTLFNGLMNNPDFQTLDFKPLILTLGGGMAVQRPVAE-----RWQHMTGCHI 382
Cdd:cd05915 229 PR--LDPASL---VELFDGEGVTFTAGVPTVWLALADYLESTGHRLKTLRRLVVGGSAAPRSLIArfermGVEVRQGYGL 303
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 383 TEGYGLS---------ETSPVACANALDATE----FTGTIGLPMPSTDVVIRDddgsdlllGK-VGEICVRGPQVMKGYW 448
Cdd:cd05915 304 TETSPVVvqnfvkshlESLSEEEKLTLKAKTglpiPLVRLRVADEEGRPVPKD--------GKaLGEVQLKGPWITGGYY 375
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 449 NRPEETARAIMADGFFRTGDMGFMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVAAEHPGVLESAAVGIPNEHSGEVVK 528
Cdd:cd05915 376 GNEEATRSALTPDGFFRTGDIAVWDEEGYVEIKDRLKDLIKSGGEWISSVDLENALMGHPKVKEAAVVAIPHPKWQERPL 455
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|...
gi 479816261 529 LYVVRRDPELTEDEVKAFCAERLTNYKR-PREVEFRESLPKSNVGKILRRELR 580
Cdd:cd05915 456 AVVVPRGEKPTPEELNEHLLKAGFAKWQlPDAYVFAEEIPRTSAGKFLKRALR 508
|
|
| PRK07008 |
PRK07008 |
long-chain-fatty-acid--CoA ligase; Validated |
77-581 |
1.88e-32 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 235908 [Multi-domain] Cd Length: 539 Bit Score: 131.37 E-value: 1.88e-32
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 77 SYKELDDHSRALAAWLQSRGLVKGDRVAIMMPNILQYPITFTAVLRAGFVVVNVNPLYTPRELEHQLNDAGAKALVVLEN 156
Cdd:PRK07008 41 TYRDCERRAKQLAQALAALGVEPGDRVGTLAWNGYRHLEAYYGVSGSGAVCHTINPRLFPEQIAYIVNHAEDRYVLFDLT 120
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 157 FATTVEKALPsiNVPNI--LVAsMGDmlgfKGHIvnlvvrrvkkmvPAWNIPghVRFKDALAQGRAKSFNPVPVQGCDIA 234
Cdd:PRK07008 121 FLPLVDALAP--QCPNVkgWVA-MTD----AAHL------------PAGSTP--LLCYETLVGAQDGDYDWPRFDENQAS 179
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 235 FLQYTGGTTGISKGAMLTH-SNIL-ANVEQMNLWMDVAFRNKGKPkalnfvcALPLYHIFALTV---NAMIGMKLgarni 309
Cdd:PRK07008 180 SLCYTSGTTGNPKGALYSHrSTVLhAYGAALPDAMGLSARDAVLP-------VVPMFHVNAWGLpysAPLTGAKL----- 247
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 310 LIPNPR-DIPSFVKELKKYPVHIFPGLNTLFNGLMNNPDFQTLDFKPLILTLGGGMAVQRPVAERWQHMTGCHITEGYGL 388
Cdd:PRK07008 248 VLPGPDlDGKSLYELIEAERVTFSAGVPTVWLGLLNHMREAGLRFSTLRRTVIGGSACPPAMIRTFEDEYGVEVIHAWGM 327
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 389 SETSPVACANALDATEFTGTI----------GLPMPSTDVVIRDDDGSDLLL-GKV-GEICVRGPQVMKGYWNRPEETar 456
Cdd:PRK07008 328 TEMSPLGTLCKLKWKHSQLPLdeqrkllekqGRVIYGVDMKIVGDDGRELPWdGKAfGDLQVRGPWVIDRYFRGDASP-- 405
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 457 aiMADGFFRTGDMGFMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVAAEHPGVLESAAVGIPNEHSGEVVKLYVVRR-D 535
Cdd:PRK07008 406 --LVDGWFPTGDVATIDADGFMQITDRSKDVIKSGGEWISSIDIENVAVAHPAVAEAACIACAHPKWDERPLLVVVKRpG 483
|
490 500 510 520
....*....|....*....|....*....|....*....|....*.
gi 479816261 536 PELTEDEVKAFCAERLTNYKRPREVEFRESLPKSNVGKILRRELRD 581
Cdd:PRK07008 484 AEVTREELLAFYEGKVAKWWIPDDVVFVDAIPHTATGKLQKLKLRE 529
|
|
| ACS |
cd05966 |
Acetyl-CoA synthetase (also known as acetate-CoA ligase and acetyl-activating enzyme); ... |
72-581 |
2.90e-32 |
|
Acetyl-CoA synthetase (also known as acetate-CoA ligase and acetyl-activating enzyme); Acetyl-CoA synthetase (ACS, EC 6.2.1.1, acetate#CoA ligase or acetate:CoA ligase (AMP-forming)) catalyzes the formation of acetyl-CoA from acetate, CoA, and ATP. Synthesis of acetyl-CoA is carried out in a two-step reaction. In the first step, the enzyme catalyzes the synthesis of acetyl-AMP intermediate from acetate and ATP. In the second step, acetyl-AMP reacts with CoA to produce acetyl-CoA. This enzyme is widely present in all living organisms. The activity of this enzyme is crucial for maintaining the required levels of acetyl-CoA, a key intermediate in many important biosynthetic and catabolic processes. Acetyl-CoA is used in the biosynthesis of glucose, fatty acids, and cholesterol. It can also be used in the production of energy in the citric acid cycle. Eukaryotes typically have two isoforms of acetyl-CoA synthetase, a cytosolic form involved in biosynthetic processes and a mitochondrial form primarily involved in energy generation.
Pssm-ID: 341270 [Multi-domain] Cd Length: 608 Bit Score: 131.53 E-value: 2.90e-32
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 72 MGKDISYKELDDHSRALAAWLQSRGLVKGDRVAIMMPNILQYPITFTAVLRAGFVVVNVNPLYTPRELEHQLNDAGAKAL 151
Cdd:cd05966 81 QSRTITYRELLREVCRFANVLKSLGVKKGDRVAIYMPMIPELVIAMLACARIGAVHSVVFAGFSAESLADRINDAQCKLV 160
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 152 VVLE---------NFATTVEKAL---PSI-NVpnilvasmgdmlgfkghivnLVVRRVKKMVPaWNIPGHVRFKDALAQG 218
Cdd:cd05966 161 ITADggyrggkviPLKEIVDEALekcPSVeKV--------------------LVVKRTGGEVP-MTEGRDLWWHDLMAKQ 219
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 219 RAKSfNPVPVQGCDIAFLQYTGGTTGISKGAMLTHSNILanveqmnLWMDVAFRNkgkpkALNfvcalplYH---IFALT 295
Cdd:cd05966 220 SPEC-EPEWMDSEDPLFILYTSGSTGKPKGVVHTTGGYL-------LYAATTFKY-----VFD-------YHpddIYWCT 279
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 296 vnAMIG------------MKLGARNIL---IPNPRDIPSFVKELKKYPVHIFPGLNTLFNGLM-------NNPDFQTLDf 353
Cdd:cd05966 280 --ADIGwitghsyivygpLANGATTVMfegTPTYPDPGRYWDIVEKHKVTIFYTAPTAIRALMkfgdewvKKHDLSSLR- 356
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 354 kpLILTLGGGMAvqrPVAERWQHMTG----CHITEGYGLSET-----SPVACANALDAteftGTIGLPMPSTDVVIRDDD 424
Cdd:cd05966 357 --VLGSVGEPIN---PEAWMWYYEVIgkerCPIVDTWWQTETggimiTPLPGATPLKP----GSATRPFFGIEPAILDEE 427
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 425 GSDLLLGKVGEICVRG--PQVMKGYWNRPEETARAIMAD--GFFRTGDMGFMDERGYTKIVDRKKDMILVSGFNVYPNEI 500
Cdd:cd05966 428 GNEVEGEVEGYLVIKRpwPGMARTIYGDHERYEDTYFSKfpGYYFTGDGARRDEDGYYWITGRVDDVINVSGHRLGTAEV 507
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 501 EEVAAEHPGVLESAAVGIPNEHSGEVVKLYVVRRD-----PELtEDEVKAFCAERLTNYKRPREVEFRESLPKSNVGKIL 575
Cdd:cd05966 508 ESALVAHPAVAEAAVVGRPHDIKGEAIYAFVTLKDgeepsDEL-RKELRKHVRKEIGPIATPDKIQFVPGLPKTRSGKIM 586
|
....*.
gi 479816261 576 RRELRD 581
Cdd:cd05966 587 RRILRK 592
|
|
| PRK06060 |
PRK06060 |
p-hydroxybenzoic acid--AMP ligase FadD22; |
76-580 |
2.96e-32 |
|
p-hydroxybenzoic acid--AMP ligase FadD22;
Pssm-ID: 180374 [Multi-domain] Cd Length: 705 Bit Score: 132.08 E-value: 2.96e-32
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 76 ISYKELDDHSRALAAWLQSRGLVKGDRVAIMMPNILQYPITFTAVLRAGFVVVNVNPlytprELEHQlndagakalvvle 155
Cdd:PRK06060 31 VTHGQIHDGAARLGEVLRNRGLSSGDRVLLCLPDSPDLVQLLLACLARGVMAFLANP-----ELHRD------------- 92
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 156 nfattvEKALPSINVPNILVASMGDMlgfkghivnlvvrrVKKMVPAWNIPGHVRFKDAlaqGRAKSFNPVPVQGCDIAF 235
Cdd:PRK06060 93 ------DHALAARNTEPALVVTSDAL--------------RDRFQPSRVAEAAELMSEA---ARVAPGGYEPMGGDALAY 149
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 236 LQYTGGTTGISKGAMLTHSNILANVEQMnlwmdvaFRN--KGKPKALNfVCALPLYHIFALTVNAMIGMKLGARNILIPN 313
Cdd:PRK06060 150 ATYTSGTTGPPKAAIHRHADPLTFVDAM-------CRKalRLTPEDTG-LCSARMYFAYGLGNSVWFPLATGGSAVINSA 221
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 314 PRDIPSFVKELKKYPVHIFPGLNTLFNGLMN--NPDfqtlDFKPLILTLGGGMAVQRPVAERW-QHMTGCHITEGYGLSE 390
Cdd:PRK06060 222 PVTPEAAAILSARFGPSVLYGVPNFFARVIDscSPD----SFRSLRCVVSAGEALELGLAERLmEFFGGIPILDGIGSTE 297
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 391 TSPVACANALDATEfTGTIGLPMPSTDVVIRDDDGSDLLLGKVGEICVRGPQVMKGYWNRPEETaraIMADGFFRTGDMG 470
Cdd:PRK06060 298 VGQTFVSNRVDEWR-LGTLGRVLPPYEIRVVAPDGTTAGPGVEGDLWVRGPAIAKGYWNRPDSP---VANEGWLDTRDRV 373
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 471 FMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVAAEHPGVLESAAVGIPNEHSGEVVKLYVVRRDPELTEDEVKAFCAER 550
Cdd:PRK06060 374 CIDSDGWVTYRCRADDTEVIGGVNVDPREVERLIIEDEAVAEAAVVAVRESTGASTLQAFLVATSGATIDGSVMRDLHRG 453
|
490 500 510
....*....|....*....|....*....|....
gi 479816261 551 ----LTNYKRPREVEFRESLPKSNVGKILRRELR 580
Cdd:PRK06060 454 llnrLSAFKVPHRFAVVDRLPRTPNGKLVRGALR 487
|
|
| A_NRPS_MycA_like |
cd05908 |
The adenylation domain of nonribosomal peptide synthetases (NRPS) similar to mycosubtilin ... |
233-580 |
6.72e-32 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS) similar to mycosubtilin synthase subunit A (MycA); The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as (amino)-acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. This family includes NRPS similar to mycosubtilin synthase subunit A (MycA). Mycosubtilin, which is characterized by a beta-amino fatty acid moiety linked to the circular heptapeptide Asn-Tyr-Asn-Gln-Pro-Ser-Asn, belongs to the iturin family of lipopeptide antibiotics. The mycosubtilin synthase subunit A (MycA) combines functional domains derived from peptide synthetases, amino transferases, and fatty acid synthases. Nonribosomal peptide synthetases are large multifunction enzymes that synthesize many therapeutically useful peptides. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and, in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341234 [Multi-domain] Cd Length: 499 Bit Score: 129.15 E-value: 6.72e-32
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 233 IAFLQYTGGTTGISKGAMLTHSNILANVEQMNLWMDVAFRNKgkpkalnFVCALPLYHIFALTVNAMIGMKLGARNILIP 312
Cdd:cd05908 108 LAFIQFSSGSTGDPKGVMLTHENLVHNMFAILNSTEWKTKDR-------ILSWMPLTHDMGLIAFHLAPLIAGMNQYLMP 180
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 313 NPRDI--PS-FVKELKKYPVHIFPGLN---TLFNGLMNNPDFQTLDFKPLILTLGGGMAVQRPVAERW-QHMTGCHITEG 385
Cdd:cd05908 181 TRLFIrrPIlWLKKASEHKATIVSSPNfgyKYFLKTLKPEKANDWDLSSIRMILNGAEPIDYELCHEFlDHMSKYGLKRN 260
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 386 -----YGLSETSPVACANALDATEFTGTI---------------------------GLPMPSTDVVIRDDDGSDLLLGKV 433
Cdd:cd05908 261 ailpvYGLAEASVGASLPKAQSPFKTITLgrrhvthgepepevdkkdsecltfvevGKPIDETDIRICDEDNKILPDGYI 340
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 434 GEICVRGPQVMKGYWNRPEETARAIMADGFFRTGDMGFM-DERGYtkIVDRKKDMILVSGFNVYPNEIEEVAAEHPGVL- 511
Cdd:cd05908 341 GHIQIRGKNVTPGYYNNPEATAKVFTDDGWLKTGDLGFIrNGRLV--ITGREKDIIFVNGQNVYPHDIERIAEELEGVEl 418
|
330 340 350 360 370 380 390
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 479816261 512 -ESAAVGIPNEHS-GEVVKLYVVRRDpelTEDEVKAFCAE--RLTNYK---RPREVEFRESLPKSNVGKILRRELR 580
Cdd:cd05908 419 gRVVACGVNNSNTrNEEIFCFIEHRK---SEDDFYPLGKKikKHLNKRggwQINEVLPIRRIPKTTSGKVKRYELA 491
|
|
| PRK12316 |
PRK12316 |
peptide synthase; Provisional |
52-579 |
1.14e-31 |
|
peptide synthase; Provisional
Pssm-ID: 237054 [Multi-domain] Cd Length: 5163 Bit Score: 131.62 E-value: 1.14e-31
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 52 ISDMISASCRQFANSPAFTCMGKDISYKELDDHSRALAAWLQSRGLVKGDRVAIMMPNILQYPITFTAVLRAGFVVVNVN 131
Cdd:PRK12316 2005 VHQRIAEQAARAPEAIAVVFGDQHLSYAELDSRANRLAHRLRARGVGPEVRVAIAAERSFELVVALLAVLKAGGAYVPLD 2084
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 132 PLYtPRE-LEHQLNDAGAKALVVLENFAttvEKALPSINVPNILVASMGDMLGFKGHivnlvvrrvkkmvpawnipghvr 210
Cdd:PRK12316 2085 PNY-PAErLAYMLEDSGAALLLTQRHLL---ERLPLPAGVARLPLDRDAEWADYPDT----------------------- 2137
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 211 fkdalaqgraksfNP-VPVQGCDIAFLQYTGGTTGISKGAMLTHSNILANVEqmnlWMDVAFRNKGKPKALNFVCalply 289
Cdd:PRK12316 2138 -------------APaVQLAGENLAYVIYTSGSTGLPKGVAVSHGALVAHCQ----AAGERYELSPADCELQFMS----- 2195
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 290 hiFALTVNA---MIGMKLGARNILIPNP-RDIPSFVKELKKYPVHI--FPG--LNTLFNGLM---NNPDFQTLDFkplil 358
Cdd:PRK12316 2196 --FSFDGAHeqwFHPLLNGARVLIRDDElWDPEQLYDEMERHGVTIldFPPvyLQQLAEHAErdgRPPAVRVYCF----- 2268
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 359 tlgGGMAVQRPVAER-WQHMTGCHITEGYGLSETSPVACANALDATEFTGT----IGLPMPSTDVVIRDDDGSDLLLGKV 433
Cdd:PRK12316 2269 ---GGEAVPAASLRLaWEALRPVYLFNGYGPTEAVVTPLLWKCRPQDPCGAayvpIGRALGNRRAYILDADLNLLAPGMA 2345
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 434 GEICVRGPQVMKGYWNRPEETARAIMADGF-------FRTGDMGFMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVAAE 506
Cdd:PRK12316 2346 GELYLGGEGLARGYLNRPGLTAERFVPDPFsasgerlYRTGDLARYRADGVVEYLGRIDHQVKIRGFRIELGEIEARLQA 2425
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 479816261 507 HPGVLESAAVGIPNEhSGEVVKLYVVRRDP-ELTEDEVKAFCAERLTNYKRPREVEFRESLPKSNVGKILRREL 579
Cdd:PRK12316 2426 HPAVREAVVVAQDGA-SGKQLVAYVVPDDAaEDLLAELRAWLAARLPAYMVPAHWVVLERLPLNPNGKLDRKAL 2498
|
|
| PRK08308 |
PRK08308 |
acyl-CoA synthetase; Validated |
236-579 |
2.82e-31 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236231 [Multi-domain] Cd Length: 414 Bit Score: 125.92 E-value: 2.82e-31
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 236 LQYTGGTTGISKGAMLTHSNILANVEQMNlwmdVAFRnkgKPKALNFVCALPLYHIFALTVNAMIGMKLGARNILI--PN 313
Cdd:PRK08308 106 LQYSSGTTGEPKLIRRSWTEIDREIEAYN----EALN---CEQDETPIVACPVTHSYGLICGVLAALTRGSKPVIItnKN 178
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 314 PRDIpsfVKELKKYPVHIFPGLNTLFNGLMNnpdfqtldFKPLILTLGGGMAVQRPVAERW----QHMTGcHITEGYGLS 389
Cdd:PRK08308 179 PKFA---LNILRNTPQHILYAVPLMLHILGR--------LLPGTFQFHAVMTSGTPLPEAWfyklRERTT-YMMQQYGCS 246
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 390 ETSPVA-CANALDATEftgtIGLPMPSTDVVIrdddGSDLllGKVGEICVRgpqvmkgywnrpeetaraiMADGFFRTGD 468
Cdd:PRK08308 247 EAGCVSiCPDMKSHLD----LGNPLPHVSVSA----GSDE--NAPEEIVVK-------------------MGDKEIFTKD 297
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 469 MGFMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVAAEHPGVLESAAVGIPNEHSGEVVKLYVVRRDpELTEDEVKAFCA 548
Cdd:PRK08308 298 LGYKSERGTLHFMGRMDDVINVSGLNVYPIEVEDVMLRLPGVQEAVVYRGKDPVAGERVKAKVISHE-EIDPVQLREWCI 376
|
330 340 350
....*....|....*....|....*....|.
gi 479816261 549 ERLTNYKRPREVEFRESLPKSNVGKILRREL 579
Cdd:PRK08308 377 QHLAPYQVPHEIESVTEIPKNANGKVSRKLL 407
|
|
| PRK05850 |
PRK05850 |
acyl-CoA synthetase; Validated |
51-556 |
4.18e-31 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 235624 [Multi-domain] Cd Length: 578 Bit Score: 127.75 E-value: 4.18e-31
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 51 SISDMISASCRQFANSPAFTCMGKDISYKELDD-------HSRA--LAAWLQSRGlVKGDRVAIMMPNILQYPITFTAVL 121
Cdd:PRK05850 2 SVPSLLRERASLQPDDAAFTFIDYEQDPAGVAEtltwsqlYRRTlnVAEELRRHG-STGDRAVILAPQGLEYIVAFLGAL 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 122 RAGFVVVnvnPLYTPRELEHQlndagakalvvlENFATTVEKALPSInvpnILVASMgdmlgfkghivnlVVRRVKKMV- 200
Cdd:PRK05850 81 QAGLIAV---PLSVPQGGAHD------------ERVSAVLRDTSPSV----VLTTSA-------------VVDDVTEYVa 128
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 201 --PAWNIPGHVRFkDALAQGRAKSFNPVPVQGCDIAFLQYTGGTTGISKGAMLTHSNILANVEQMnlwMDVAFRNKGK-- 276
Cdd:PRK05850 129 pqPGQSAPPVIEV-DLLDLDSPRGSDARPRDLPSTAYLQYTSGSTRTPAGVMVSHRNVIANFEQL---MSDYFGDTGGvp 204
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 277 PKALNFVCALPLYHIFALTVNAMIGMKLGARNILI--------P---------NPRDI---PSFVKEL--KKypvhifpg 334
Cdd:PRK05850 205 PPDTTVVSWLPFYHDMGLVLGVCAPILGGCPAVLTspvaflqrParwmqllasNPHAFsaaPNFAFELavRK-------- 276
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 335 lntlfnglMNNPDFQTLDFKPLILTLGGGMAVQ----RPVAERWQHMtGCH---ITEGYGLSE-TSPVACANA---LDAT 403
Cdd:PRK05850 277 --------TSDDDMAGLDLGGVLGIISGSERVHpatlKRFADRFAPF-NLRetaIRPSYGLAEaTVYVATREPgqpPESV 347
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 404 EF----------------TGT----IGLPMPSTdVVIRD-DDGSDLLLGKVGEICVRGPQVMKGYWNRPEETARAIMA-- 460
Cdd:PRK05850 348 RFdyeklsaghakrcetgGGTplvsYGSPRSPT-VRIVDpDTCIECPAGTVGEIWVHGDNVAAGYWQKPEETERTFGAtl 426
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 461 --------DG-FFRTGDMGFMDErGYTKIVDRKKDMILVSGFNVYPNEIEEVAAEHPG--VlesAAVGIPNEHSGEVVKL 529
Cdd:PRK05850 427 vdpspgtpEGpWLRTGDLGFISE-GELFIVGRIKDLLIVDGRNHYPDDIEATIQEITGgrV---AAISVPDDGTEKLVAI 502
|
570 580
....*....|....*....|....*..
gi 479816261 530 YVVRRDPELTEDEvkafcAERLTNYKR 556
Cdd:PRK05850 503 IELKKRGDSDEEA-----MDRLRTVKR 524
|
|
| PRK06814 |
PRK06814 |
acyl-[ACP]--phospholipid O-acyltransferase; |
73-574 |
6.27e-31 |
|
acyl-[ACP]--phospholipid O-acyltransferase;
Pssm-ID: 235865 [Multi-domain] Cd Length: 1140 Bit Score: 128.93 E-value: 6.27e-31
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 73 GKDISYKELDDHSRALAAWLQsRGLVKGDRVAIMMPNILQYPITFTAVLRAGFVVVNVNPLYTPRELEHQLNDAGAKALV 152
Cdd:PRK06814 656 NGPLTYRKLLTGAFVLGRKLK-KNTPPGENVGVMLPNANGAAVTFFALQSAGRVPAMINFSAGIANILSACKAAQVKTVL 734
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 153 VLENFattVEKAlpsinvpnilvaSMGDM---LGFKGHIVNLvvRRVKKMVPAWNipghvrfK-DALAQGRAKSFNPVPV 228
Cdd:PRK06814 735 TSRAF---IEKA------------RLGPLieaLEFGIRIIYL--EDVRAQIGLAD-------KiKGLLAGRFPLVYFCNR 790
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 229 QGCDIAFLQYTGGTTGISKGAMLTHSNILANVEQmnlwmdVAFRNKGKPKALNFvCALPLYHIFALTVNAMIGMKLGARN 308
Cdd:PRK06814 791 DPDDPAVILFTSGSEGTPKGVVLSHRNLLANRAQ------VAARIDFSPEDKVF-NALPVFHSFGLTGGLVLPLLSGVKV 863
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 309 ILIPNP---RDIPSFVkelkkYPVH--IFPGLNTLFNGL--MNNPdfqtLDFKPLILTLGGGMAVQRPVAERWQHMTGCH 381
Cdd:PRK06814 864 FLYPSPlhyRIIPELI-----YDTNatILFGTDTFLNGYarYAHP----YDFRSLRYVFAGAEKVKEETRQTWMEKFGIR 934
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 382 ITEGYGLSETSPVACANALDATEFtGTIGLPMPstdvvirdddGSDLLLGKV------GEICVRGPQVMKGYWNrpEETA 455
Cdd:PRK06814 935 ILEGYGVTETAPVIALNTPMHNKA-GTVGRLLP----------GIEYRLEPVpgidegGRLFVRGPNVMLGYLR--AENP 1001
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 456 RAI--MADGFFRTGDMGFMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVAAE-HPGVLeSAAVGIPNEHSGEVVKLYVV 532
Cdd:PRK06814 1002 GVLepPADGWYDTGDIVTIDEEGFITIKGRAKRFAKIAGEMISLAAVEELAAElWPDAL-HAAVSIPDARKGERIILLTT 1080
|
490 500 510 520
....*....|....*....|....*....|....*....|...
gi 479816261 533 RRDpeLTEDEVKAFCAER-LTNYKRPREVEFRESLPKSNVGKI 574
Cdd:PRK06814 1081 ASD--ATRAAFLAHAKAAgASELMVPAEIITIDEIPLLGTGKI 1121
|
|
| PRK07768 |
PRK07768 |
long-chain-fatty-acid--CoA ligase; Validated |
84-578 |
6.87e-31 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 236091 [Multi-domain] Cd Length: 545 Bit Score: 126.65 E-value: 6.87e-31
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 84 HSRAL--AAWLQSRGLVKGDRVAIM--MPnILQYPITFTAVLRAGFVVVNVNPlyTPR-ELEHQLNDA-------GAKAL 151
Cdd:PRK07768 36 HERARriAGGLAAAGVGPGDAVAVLagAP-VEIAPTAQGLWMRGASLTMLHQP--TPRtDLAVWAEDTlrvigmiGAKAV 112
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 152 VVLENFattvEKALPSINVPNILVASMGDMLGfkghivnlvvrrvkkmvpawnipghvrfkdalaqgrAKSFNPVPVQGC 231
Cdd:PRK07768 113 VVGEPF----LAAAPVLEEKGIRVLTVADLLA------------------------------------ADPIDPVETGED 152
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 232 DIAFLQYTGGTTGISKGAMLTHSNILANVEQMNLwmdvafRNKGKPKALNFVCALPLYHIFALTVNAMIGMKLGARNILI 311
Cdd:PRK07768 153 DLALMQLTSGSTGSPKAVQITHGNLYANAEAMFV------AAEFDVETDVMVSWLPLFHDMGMVGFLTVPMYFGAELVKV 226
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 312 pNP----RDIPSFVKELKKYPVHIFPGLNTLFN----GLMNNPDFQTLDFKPLILTLGGGMAVQRPVAERWQHMTGCH-- 381
Cdd:PRK07768 227 -TPmdflRDPLLWAELISKYRGTMTAAPNFAYAllarRLRRQAKPGAFDLSSLRFALNGAEPIDPADVEDLLDAGARFgl 305
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 382 ----ITEGYGLSET------SPV--------ACANALDATEFT-----------GTIGLPMPSTDVVIRDDDGSDLLLGK 432
Cdd:PRK07768 306 rpeaILPAYGMAEAtlavsfSPCgaglvvdeVDADLLAALRRAvpatkgntrrlATLGPPLPGLEVRVVDEDGQVLPPRG 385
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 433 VGEICVRGPQVMKGYWNrPEETARAIMADGFFRTGDMGFMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVAAEHPGVLE 512
Cdd:PRK07768 386 VGVIELRGESVTPGYLT-MDGFIPAQDADGWLDTGDLGYLTEEGEVVVCGRVKDVIIMAGRNIYPTDIERAAARVEGVRP 464
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 479816261 513 --SAAVGIPNEHSGE--VVKLYVVRRDPELTEDEVKAFCAERLTNY--KRPREVEFRE--SLPKSNVGKiLRRE 578
Cdd:PRK07768 465 gnAVAVRLDAGHSREgfAVAVESNAFEDPAEVRRIRHQVAHEVVAEvgVRPRNVVVLGpgSIPKTPSGK-LRRA 537
|
|
| PRK12476 |
PRK12476 |
putative fatty-acid--CoA ligase; Provisional |
75-580 |
9.58e-31 |
|
putative fatty-acid--CoA ligase; Provisional
Pssm-ID: 171527 [Multi-domain] Cd Length: 612 Bit Score: 126.78 E-value: 9.58e-31
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 75 DISYKELDDHSRALAAWLQsRGLVKGDRVAIMMPNILQYPITFTAVLRAGFVVVnvnPLYTPR------ELEHQLNDAga 148
Cdd:PRK12476 68 ELTWTQLGVRLRAVGARLQ-QVAGPGDRVAILAPQGIDYVAGFFAAIKAGTIAV---PLFAPElpghaeRLDTALRDA-- 141
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 149 KALVVLENFAT--TVEK---ALPSINVPNILVAsmgdmlgfkghivnlvvrrvkkmvpawnipghvrfkDALAQGRAKSF 223
Cdd:PRK12476 142 EPTVVLTTTAAaeAVEGflrNLPRLRRPRVIAI------------------------------------DAIPDSAGESF 185
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 224 NPVPVQGCDIAFLQYTGGTTGISKGAMLTHSNILANVEQMNLWMDVAFRNkgkpkaLNFVCALPLYHIFALTvnaMIGMK 303
Cdd:PRK12476 186 VPVELDTDDVSHLQYTSGSTRPPVGVEITHRAVGTNLVQMILSIDLLDRN------THGVSWLPLYHDMGLS---MIGFP 256
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 304 L--GARNILIpnprDIPSFVKELKKYPVHIFPGLNT--LFNGLMN-----------NPDFQTLDFKPLILTLGG---GMA 365
Cdd:PRK12476 257 AvyGGHSTLM----SPTAFVRRPQRWIKALSEGSRTgrVVTAAPNfayewaaqrglPAEGDDIDLSNVVLIIGSepvSID 332
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 366 VQRPVAERWQ--HMTGCHITEGYGLSE-------TSPVACANA--LDATEFTG-----------------TIGLPMPSTD 417
Cdd:PRK12476 333 AVTTFNKAFApyGLPRTAFKPSYGIAEatlfvatIAPDAEPSVvyLDREQLGAgravrvaadapnavahvSCGQVARSQW 412
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 418 VVIRDDD-GSDLLLGKVGEICVRGPQVMKGYWNRPEET------------ARAIMADG------FFRTGDMGF-MDERGY 477
Cdd:PRK12476 413 AVIVDPDtGAELPDGEVGEIWLHGDNIGRGYWGRPEETertfgaklqsrlAEGSHADGaaddgtWLRTGDLGVyLDGELY 492
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 478 tkIVDRKKDMILVSGFNVYPNEIEEVAAEHPGVLES---AAVGIPNEHSGEVVklyVV--------RRDPELTEDEVKAF 546
Cdd:PRK12476 493 --ITGRIADLIVIDGRNHYPQDIEATVAEASPMVRRgyvTAFTVPAEDNERLV---IVaeraagtsRADPAPAIDAIRAA 567
|
570 580 590
....*....|....*....|....*....|....*.
gi 479816261 547 CAERLTnyKRPREVEFRES--LPKSNVGKILRRELR 580
Cdd:PRK12476 568 VSRRHG--LAVADVRLVPAgaIPRTTSGKLARRACR 601
|
|
| PRK04813 |
PRK04813 |
D-alanine--poly(phosphoribitol) ligase subunit DltA; |
52-579 |
1.18e-30 |
|
D-alanine--poly(phosphoribitol) ligase subunit DltA;
Pssm-ID: 235313 [Multi-domain] Cd Length: 503 Bit Score: 125.39 E-value: 1.18e-30
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 52 ISDMISAsCRQFANS----PAFTCMGKDISYKELDDHSRALAAWLQSRGLVKGDRVAI---MMPNILqypITFTAVLRAG 124
Cdd:PRK04813 1 IMDIIET-IEEFAQTqpdfPAYDYLGEKLTYGQLKEDSDALAAFIDSLKLPDKSPIIVfghMSPEML---ATFLGAVKAG 76
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 125 FVVVNVNpLYTPRE-LEHQLNDAGAKALVVLENFATTVEKaLPSINVPNIlvasmgdmlgfkghivnlvvrrvkkmvpaw 203
Cdd:PRK04813 77 HAYIPVD-VSSPAErIEMIIEVAKPSLIIATEELPLEILG-IPVITLDEL------------------------------ 124
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 204 nipghvrfKDALAQGRAKSFNPvPVQGCDIAFLQYTGGTTGISKGAMLTHSNILANVEqmnlWMDVAFRNKGKPKALNfv 283
Cdd:PRK04813 125 --------KDIFATGNPYDFDH-AVKGDDNYYIIFTSGTTGKPKGVQISHDNLVSFTN----WMLEDFALPEGPQFLN-- 189
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 284 caLPLYHiFALTVNAmIGMKLGARNILIPNPRDIPSFVKEL----KKYPVHIF---PG------LNTLFNGlMNNPDFQT 350
Cdd:PRK04813 190 --QAPYS-FDLSVMD-LYPTLASGGTLVALPKDMTANFKQLfetlPQLPINVWvstPSfadmclLDPSFNE-EHLPNLTH 264
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 351 LDFKPLILTlgggMAVQRPVAERWQHmtgCHITEGYGLSETSpVAcanaLDATEFTGT---------IGLPMPSTDVVIR 421
Cdd:PRK04813 265 FLFCGEELP----HKTAKKLLERFPS---ATIYNTYGPTEAT-VA----VTSIEITDEmldqykrlpIGYAKPDSPLLII 332
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 422 DDDGSDLLLGKVGEICVRGPQVMKGYWNRPEETARA-IMADG--FFRTGDMGFMDErgytkivdrkkDMILVSG------ 492
Cdd:PRK04813 333 DEEGTKLPDGEQGEIVISGPSVSKGYLNNPEKTAEAfFTFDGqpAYHTGDAGYLED-----------GLLFYQGridfqi 401
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 493 -FNVYPNEIEEVaaEH-----PGVleSAAVGIPNEHSGEVVKL--YVV------RRDPELTEdEVKAFCAERLTNYKRPR 558
Cdd:PRK04813 402 kLNGYRIELEEI--EQnlrqsSYV--ESAVVVPYNKDHKVQYLiaYVVpkeedfEREFELTK-AIKKELKERLMEYMIPR 476
|
570 580
....*....|....*....|.
gi 479816261 559 EVEFRESLPKSNVGKILRREL 579
Cdd:PRK04813 477 KFIYRDSLPLTPNGKIDRKAL 497
|
|
| A_NRPS_PvdJ-like |
cd17649 |
non-ribosomal peptide synthetase; This family of the adenylation (A) domain of nonribosomal ... |
67-580 |
3.07e-30 |
|
non-ribosomal peptide synthetase; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes pyoverdine biosynthesis protein PvdJ involved in the synthesis of pyoverdine, which consists of a chromophore group attached to a variable peptide chain and comprises around 6-12 amino acids that are specific for each Pseudomonas species, and for which the peptide might be first synthesized before the chromophore assembly. Also included is ornibactin biosynthesis protein OrbI; ornibactin is a tetrapeptide siderophore with an l-ornithine-d-hydroxyaspartate-l-serine-l-ornithine backbone. The adenylation domain at the N-terminal of OrbI possibly initiates the ornibactin with the binding of N5-hydroxyornithine. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341304 [Multi-domain] Cd Length: 450 Bit Score: 123.63 E-value: 3.07e-30
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 67 PAFTCMGKDISYKELDDHSRALAAWLQSRGLVKGDRVAIMMPNILQYPITFTAVLRAGFVVVNVNPLYTPRELEHQLNDA 146
Cdd:cd17649 4 VALVFGDQSLSYAELDARANRLAHRLRALGVGPEVRVGIALERSLEMVVALLAILKAGGAYVPLDPEYPAERLRYMLEDS 83
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 147 GAKalvvlenfattvekalpsinvpnILVASMGDMLgfkghivnlvvrrvkkmvpawnipghvrfkdalaqgraksfnpv 226
Cdd:cd17649 84 GAG-----------------------LLLTHHPRQL-------------------------------------------- 96
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 227 pvqgcdiAFLQYTGGTTGISKGAMLTHSNILANVEQMNLWMDVAFRNKGKPKA-LNFVCALP-LYHifALTVNAMIGM-- 302
Cdd:cd17649 97 -------AYVIYTSGSTGTPKGVAVSHGPLAAHCQATAERYGLTPGDRELQFAsFNFDGAHEqLLP--PLICGACVVLrp 167
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 303 ---------------KLGARNILIPnprdiPSFVKELKKYPVHIFPGlntlfnglmnnpdfqtlDFKPLILTLGGGMAVQ 367
Cdd:cd17649 168 delwasadelaemvrELGVTVLDLP-----PAYLQQLAEEADRTGDG-----------------RPPSLRLYIFGGEALS 225
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 368 RPVAERWQhMTGCHITEGYGLSET--SPVACANALDATEFTGT--IGLPMPSTDVVIRDDDGSDLLLGKVGEICVRGPQV 443
Cdd:cd17649 226 PELLRRWL-KAPVRLFNAYGPTEAtvTPLVWKCEAGAARAGASmpIGRPLGGRSAYILDADLNPVPVGVTGELYIGGEGL 304
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 444 MKGYWNRPEETARAIMADGFF-------RTGDMGFMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVAAEHPGVLESAAV 516
Cdd:cd17649 305 ARGYLGRPELTAERFVPDPFGapgsrlyRTGDLARWRDDGVIEYLGRVDHQVKIRGFRIELGEIEAALLEHPGVREAAVV 384
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 479816261 517 GIPNEHSGEVVKLYVVRRDPELTED--EVKAFCAERLTNYKRPREVEFRESLPKSNVGKILRRELR 580
Cdd:cd17649 385 ALDGAGGKQLVAYVVLRAAAAQPELraQLRTALRASLPDYMVPAHLVFLARLPLTPNGKLDRKALP 450
|
|
| PRK12467 |
PRK12467 |
peptide synthase; Provisional |
41-579 |
3.60e-30 |
|
peptide synthase; Provisional
Pssm-ID: 237108 [Multi-domain] Cd Length: 3956 Bit Score: 126.81 E-value: 3.60e-30
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 41 PHDINLSAATSISDMISASCRQFANSPAFTCMGKDISYKELDDHSRALAAWLQSRGLVKGDRVAIMMPNILQYPITFTAV 120
Cdd:PRK12467 1565 ATHTGYPLARLVHQLIEDQAAATPEAVALVFGEQELTYGELNRRANRLAHRLIALGVGPEVLVGIAVERSLEMVVGLLAI 1644
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 121 LRAGFVVVNVNPLYTPRELEHQLNDAGAKALvvLENFAttVEKALPSINVPNILVASMGDmlgfkghivnlvvrrvkkmv 200
Cdd:PRK12467 1645 LKAGGAYVPLDPEYPRERLAYMIEDSGIELL--LTQSH--LQARLPLPDGLRSLVLDQED-------------------- 1700
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 201 pAWnipghvrfkdalAQGRAKSfNP-VPVQGCDIAFLQYTGGTTGISKGAMLTHSNILANVEqmnlWMDVAFRNKGKPKA 279
Cdd:PRK12467 1701 -DW------------LEGYSDS-NPaVNLAPQNLAYVIYTSGSTGRPKGAGNRHGALVNRLC----ATQEAYQLSAADVV 1762
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 280 LNFvcalplyHIFALTVNA---MIGMKLGARNILIPN--PRDIPSFVKELKKYPVHIFPGLNTLFNGLMNNPDFQTLDFK 354
Cdd:PRK12467 1763 LQF-------TSFAFDVSVwelFWPLINGARLVIAPPgaHRDPEQLIQLIERQQVTTLHFVPSMLQQLLQMDEQVEHPLS 1835
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 355 PLILTLGG---GMAVQRPVAERwqhMTGCHITEGYGLSETspvacanALDATEFTGT-----------IGLPMPSTDVVI 420
Cdd:PRK12467 1836 LRRVVCGGealEVEALRPWLER---LPDTGLFNLYGPTET-------AVDVTHWTCRrkdlegrdsvpIGQPIANLSTYI 1905
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 421 RDDDGSDLLLGKVGEICVRGPQVMKGYWNRPEETARAIMADGF-------FRTGDMGFMDERGYTKIVDRKKDMILVSGF 493
Cdd:PRK12467 1906 LDASLNPVPIGVAGELYLGGVGLARGYLNRPALTAERFVADPFgtvgsrlYRTGDLARYRADGVIEYLGRIDHQVKIRGF 1985
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 494 NVYPNEIEEVAAEHPGVLESAAVGIPNEHSGEVVKlYVVRRDPELTEDEV---------KAFCAERLTNYKRPREVEFRE 564
Cdd:PRK12467 1986 RIELGEIEARLREQGGVREAVVIAQDGANGKQLVA-YVVPTDPGLVDDDEaqvalrailKNHLKASLPEYMVPAHLVFLA 2064
|
570
....*....|....*
gi 479816261 565 SLPKSNVGKILRREL 579
Cdd:PRK12467 2065 RMPLTPNGKLDRKAL 2079
|
|
| PLN02736 |
PLN02736 |
long-chain acyl-CoA synthetase |
54-513 |
4.07e-30 |
|
long-chain acyl-CoA synthetase
Pssm-ID: 178337 [Multi-domain] Cd Length: 651 Bit Score: 125.21 E-value: 4.07e-30
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 54 DMISASCRQFANSPaftCMGKDI------------SYKELDDHSRALAAWLQSRGLVKGDRVAIMMPNILQYPITFTAVL 121
Cdd:PLN02736 48 DNFVYAVETFRDYK---YLGTRIrvdgtvgeykwmTYGEAGTARTAIGSGLVQHGIPKGACVGLYFINRPEWLIVDHACS 124
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 122 RAGFVVVnvnPLYtprelehqlNDAGAKALVVLENFATtVEKALPSINVPNILVASMGDMLGFKgHIVnlVVRRVKKMVP 201
Cdd:PLN02736 125 AYSYVSV---PLY---------DTLGPDAVKFIVNHAE-VAAIFCVPQTLNTLLSCLSEIPSVR-LIV--VVGGADEPLP 188
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 202 AwnIPGH-----VRFKDALAQGRAKSFNPVPVQGCDIAFLQYTGGTTGISKGAMLTHSNILANVEQMNLWM-----DVaf 271
Cdd:PLN02736 189 S--LPSGtgveiVTYSKLLAQGRSSPQPFRPPKPEDVATICYTSGTTGTPKGVVLTHGNLIANVAGSSLSTkfypsDV-- 264
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 272 rnkgkpkalnFVCALPLYHIFAlTVNAMIGMKLGAR--------------------NILIPNPR-------DIPSFVKE- 323
Cdd:PLN02736 265 ----------HISYLPLAHIYE-RVNQIVMLHYGVAvgfyqgdnlklmddlaalrpTIFCSVPRlynriydGITNAVKEs 333
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 324 --LKKYPVHIfpGLNT----LFNGLMNNPDFQTLDFKPLILTLGG-------GMAVQRP-VAERWQHMTGCHITEGYGLS 389
Cdd:PLN02736 334 ggLKERLFNA--AYNAkkqaLENGKNPSPMWDRLVFNKIKAKLGGrvrfmssGASPLSPdVMEFLRICFGGRVLEGYGMT 411
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 390 ETSPVAcaNALDATEFT-GTIGLPMPSTDVVIRD----DDGSDLLLGKVGEICVRGPQVMKGYWNRPEETARAIMADGFF 464
Cdd:PLN02736 412 ETSCVI--SGMDEGDNLsGHVGSPNPACEVKLVDvpemNYTSEDQPYPRGEICVRGPIIFKGYYKDEVQTREVIDEDGWL 489
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|
gi 479816261 465 RTGDMGFMDERGYTKIVDRKKDMI-LVSGFNVYPNEIEEVAAEHPGVLES 513
Cdd:PLN02736 490 HTGDIGLWLPGGRLKIIDRKKNIFkLAQGEYIAPEKIENVYAKCKFVAQC 539
|
|
| PRK12467 |
PRK12467 |
peptide synthase; Provisional |
43-579 |
5.43e-30 |
|
peptide synthase; Provisional
Pssm-ID: 237108 [Multi-domain] Cd Length: 3956 Bit Score: 126.43 E-value: 5.43e-30
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 43 DINLSAATSISD----MISASCRQFANSPAFTCMGKDISYKELDDHSRALAAWLQSRGLVKGDRVAIMMPNILQYPITFT 118
Cdd:PRK12467 501 RWNAPATEYAPDcvhqLIEAQARQHPERPALVFGEQVLSYAELNRQANRLAHVLIAAGVGPDVLVGIAVERSIEMVVGLL 580
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 119 AVLRAGFVVVNVNPLYtPRE-LEHQLNDAGAKALVvlenfatTVEKALPSINVPNILVASMGDMLgfkghivnlvvrrvk 197
Cdd:PRK12467 581 AVLKAGGAYVPLDPEY-PQDrLAYMLDDSGVRLLL-------TQSHLLAQLPVPAGLRSLCLDEP--------------- 637
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 198 kmvpawnipghvrfkDALAQGRAKSFNPVPVQGCDIAFLQYTGGTTGISKGAMLTHSNILANVEQMNLWMDVAFRNKgkp 277
Cdd:PRK12467 638 ---------------ADLLCGYSGHNPEVALDPDNLAYVIYTSGSTGQPKGVAISHGALANYVCVIAERLQLAADDS--- 699
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 278 kalnfvcaLPLYHIFA--LTVNAMIG-MKLGARNILIP--NPRDIPSFVKELKKYPVHIFPGLNTLFNGLMNNPdfQTLD 352
Cdd:PRK12467 700 --------MLMVSTFAfdLGVTELFGaLASGATLHLLPpdCARDAEAFAALMADQGVTVLKIVPSHLQALLQAS--RVAL 769
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 353 FKPLILTLGGGMAVQRPVAERW-QHMTGCHITEGYGLSETSPVACANAL--DATEFTGT-IGLPMPSTDVVIRDDDGSDL 428
Cdd:PRK12467 770 PRPQRALVCGGEALQVDLLARVrALGPGARLINHYGPTETTVGVSTYELsdEERDFGNVpIGQPLANLGLYILDHYLNPV 849
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 429 LLGKVGEICVRGPQVMKGYWNRPEETARAIMADGF-------FRTGDMGFMDERGYTKIVDRKKDMILVSGFNVYPNEIE 501
Cdd:PRK12467 850 PVGVVGELYIGGAGLARGYHRRPALTAERFVPDPFgadggrlYRTGDLARYRADGVIEYLGRMDHQVKIRGFRIELGEIE 929
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 502 EVAAEHPGVLESAAVGIPNEHSGEVVKLYVVRRDPELTE-----DEVKAFCAERLTNYKRPREVEFRESLPKSNVGKILR 576
Cdd:PRK12467 930 ARLLAQPGVREAVVLAQPGDAGLQLVAYLVPAAVADGAEhqatrDELKAQLRQVLPDYMVPAHLLLLDSLPLTPNGKLDR 1009
|
...
gi 479816261 577 REL 579
Cdd:PRK12467 1010 KAL 1012
|
|
| PRK07769 |
PRK07769 |
long-chain-fatty-acid--CoA ligase; Validated |
75-580 |
1.48e-29 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 181109 [Multi-domain] Cd Length: 631 Bit Score: 123.30 E-value: 1.48e-29
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 75 DISYKELDDHSRALAAWLQSRGlVKGDRVAIMMPNILQYPITFTAVLRAGFVVVnvnPLYTPRELEHQ------LNDAGA 148
Cdd:PRK07769 55 DLTWSQFGARNRAVGARLQQVT-KPGDRVAILAPQNLDYLIAFFGALYAGRIAV---PLFDPAEPGHVgrlhavLDDCTP 130
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 149 KALVvlenfaTTVEKAlpsinvpnilvasmgdmlgfKGhivnlvVRRVKKMVPAWNIPgHVRFKDALAQGRAKSFNPVPV 228
Cdd:PRK07769 131 SAIL------TTTDSA--------------------EG------VRKFFRARPAKERP-RVIAVDAVPDEVGATWVPPEA 177
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 229 QGCDIAFLQYTGGTTGISKGAMLTHSNILANVEQMNLWMDVAFRNKGkpkalnfVCALPLYHIFAL-TVnamIGMKLGAR 307
Cdd:PRK07769 178 NEDTIAYLQYTSGSTRIPAGVQITHLNLPTNVLQVIDALEGQEGDRG-------VSWLPFFHDMGLiTV---LLPALLGH 247
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 308 NILIPNPRdipSFV-------KELKKYPvhifPGLNTLFNGLMN------------NPDFQTLDFKPLILTLGGGMAVQR 368
Cdd:PRK07769 248 YITFMSPA---AFVrrpgrwiRELARKP----GGTGGTFSAAPNfafehaaarglpKDGEPPLDLSNVKGLLNGSEPVSP 320
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 369 PVAERWqhmtgCHITEGYGLSETSPVACANALDATEFTGTIG------------------------------LPMPSTDV 418
Cdd:PRK07769 321 ASMRKF-----NEAFAPYGLPPTAIKPSYGMAEATLFVSTTPmdeeptviyvdrdelnagrfvevpadapnaVAQVSAGK 395
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 419 VIRD--------DDGSDLLLGKVGEICVRGPQVMKGYWNRPEETARAIM-----------ADG------FFRTGDMG-FM 472
Cdd:PRK07769 396 VGVSewavivdpETASELPDGQIGEIWLHGNNIGTGYWGKPEETAATFQnilksrlseshAEGapddalWVRTGDYGvYF 475
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 473 DERGYtkIVDRKKDMILVSGFNVYPNEIEEVAAEHPGVLES---AAVGIP----------NEHSGEVV-------KLYVV 532
Cdd:PRK07769 476 DGELY--ITGRVKDLVIIDGRNHYPQDLEYTAQEATKALRTgyvAAFSVPanqlpqvvfdDSHAGLKFdpedtseQLVIV 553
|
570 580 590 600 610 620
....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 533 --------RRDPELTEDEVKAFCAER--LTnykrPREVEFRE--SLPKSNVGKILRRELR 580
Cdd:PRK07769 554 aerapgahKLDPQPIADDIRAAIAVRhgVT----VRDVLLVPagSIPRTSSGKIARRACR 609
|
|
| PRK07867 |
PRK07867 |
acyl-CoA synthetase; Validated |
67-581 |
1.95e-29 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236120 [Multi-domain] Cd Length: 529 Bit Score: 122.10 E-value: 1.95e-29
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 67 PAFTCMGKDISYKELDDHSRALAAWLqsRGLVKGDR---VAIMMPNILQYPITFTAVLRAGFVVVNVNPLYTPRELEHQL 143
Cdd:PRK07867 20 RGLYFEDSFTSWREHIRGSAARAAAL--RARLDPTRpphVGVLLDNTPEFSLLLGAAALSGIVPVGLNPTRRGAALARDI 97
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 144 NDAGAKALVVLENFATTVEKALPsiNVPNILVASmgdmlgfkghivnlvvrrvkkmvPAWnipghvrfKDALAQGRAKSF 223
Cdd:PRK07867 98 AHADCQLVLTESAHAELLDGLDP--GVRVINVDS-----------------------PAW--------ADELAAHRDAEP 144
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 224 NPVPVQGCDIAFLQYTGGTTGISKGAMLTHSNI----LANVEQMNLWM-DVAFrnkgkpkalnfvCALPLYHIFALTVNA 298
Cdd:PRK07867 145 PFRVADPDDLFMLIFTSGTSGDPKAVRCTHRKVasagVMLAQRFGLGPdDVCY------------VSMPLFHSNAVMAGW 212
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 299 MIGMKLGArNILIPNPRDIPSFVKELKKYPVhifpglnTLFN--GlmnnpdfqtldfKPL--IL-----------TL--- 360
Cdd:PRK07867 213 AVALAAGA-SIALRRKFSASGFLPDVRRYGA-------TYANyvG------------KPLsyVLatperpddadnPLriv 272
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 361 ----GGGMAVQRpVAERWqhmtGCHITEGYGLSETSpVACANALDATEftGTIGLPMPSTDVV-----------IRDDDG 425
Cdd:PRK07867 273 ygneGAPGDIAR-FARRF----GCVVVDGFGSTEGG-VAITRTPDTPP--GALGPLPPGVAIVdpdtgtecppaEDADGR 344
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 426 SDLLLGKVGEIC-VRGPQVMKGYWNRPEETARAiMADGFFRTGDMGFMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVA 504
Cdd:PRK07867 345 LLNADEAIGELVnTAGPGGFEGYYNDPEADAER-MRGGVYWSGDLAYRDADGYAYFAGRLGDWMRVDGENLGTAPIERIL 423
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 505 AEHPGVLESAAVGIPNEHSGEVVKLYVV-RRDPELTEDEVKAFCAER--LTNYKRPREVEFRESLPKSNVGKILRRELRD 581
Cdd:PRK07867 424 LRYPDATEVAVYAVPDPVVGDQVMAALVlAPGAKFDPDAFAEFLAAQpdLGPKQWPSYVRVCAELPRTATFKVLKRQLSA 503
|
|
| PRK08043 |
PRK08043 |
bifunctional acyl-ACP--phospholipid O-acyltransferase/long-chain-fatty-acid--ACP ligase; |
226-573 |
2.06e-29 |
|
bifunctional acyl-ACP--phospholipid O-acyltransferase/long-chain-fatty-acid--ACP ligase;
Pssm-ID: 181207 [Multi-domain] Cd Length: 718 Bit Score: 123.28 E-value: 2.06e-29
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 226 VPVQGCDIAFLQYTGGTTGISKGAMLTHSNILANVEQMNLWMDVAFRNKgkpkalnFVCALPLYHIFALTVNAMIGMKLG 305
Cdd:PRK08043 360 VKQQPEDAALILFTSGSEGHPKGVVHSHKSLLANVEQIKTIADFTPNDR-------FMSALPLFHSFGLTVGLFTPLLTG 432
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 306 ARNILIPNP---RDIPSFVKElKKYPVhIFpGLNTLfngLMNNPDF-QTLDFKPLILTLGGGMAVQRPVAERWQHMTGCH 381
Cdd:PRK08043 433 AEVFLYPSPlhyRIVPELVYD-RNCTV-LF-GTSTF---LGNYARFaNPYDFARLRYVVAGAEKLQESTKQLWQDKFGLR 506
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 382 ITEGYGLSETSPVACANALDATEfTGTIGLPMPSTDV----VIRDDDGSDLLLgkvgeicvRGPQVMKGYWnRPE----- 452
Cdd:PRK08043 507 ILEGYGVTECAPVVSINVPMAAK-PGTVGRILPGMDArllsVPGIEQGGRLQL--------KGPNIMNGYL-RVEkpgvl 576
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 453 -----ETARAIMADGFFRTGDMGFMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVAAEHPGVLESAAVGIPNEHSGEVV 527
Cdd:PRK08043 577 evptaENARGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMVSLEMVEQLALGVSPDKQHATAIKSDASKGEAL 656
|
330 340 350 360
....*....|....*....|....*....|....*....|....*..
gi 479816261 528 KLYVVrrDPELTEDEVKAFCAER-LTNYKRPREVEFRESLPKSNVGK 573
Cdd:PRK08043 657 VLFTT--DSELTREKLQQYAREHgVPELAVPRDIRYLKQLPLLGSGK 701
|
|
| A_NRPS_CmdD_like |
cd17652 |
similar to adenylation domain of chondramide synthase cmdD; This family of the adenylation (A) ... |
66-579 |
2.15e-29 |
|
similar to adenylation domain of chondramide synthase cmdD; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes phosphinothricin tripeptide (PTT, phosphinothricylalanylalanine) synthetase, where PTT is a natural-product antibiotic and potent herbicide that is produced by Streptomyces hygroscopicus. This adenylation domain has been confirmed to directly activate beta-tyrosine, and fluorinated chondramides are produced through precursor-directed biosynthesis. Also included in this family is chondramide synthase D (also known as ATP-dependent phenylalanine adenylase or phenylalanine activase or tyrosine activase). Chondramides A-D are depsipeptide antitumor and antifungal antibiotics produced by C. crocatus, are a class of mixed peptide/polyketide depsipeptides comprised of three amino acids (alanine, N-methyltryptophan, plus the unusual amino acid beta-tyrosine or alpha-methoxy-beta-tyrosine) and a polyketide chain ([E]-7-hydroxy-2,4,6-trimethyloct-4-enoic acid).
Pssm-ID: 341307 [Multi-domain] Cd Length: 436 Bit Score: 120.82 E-value: 2.15e-29
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 66 SPAFTCMGKDISYKELDDHSRALAAWLQSRGLVKGDRVAIMMPNILQYPITFTAVLRAGFVVVNVNPLYTPRELEHQLND 145
Cdd:cd17652 3 APAVVFGDETLTYAELNARANRLARLLAARGVGPERLVALALPRSAELVVAILAVLKAGAAYLPLDPAYPAERIAYMLAD 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 146 AGAKALVvlenfaTTVEkalpsinvpnilvasmgdmlgfkghivnlvvrrvkkmvpawnipghvrfkdalaqgraksfnp 225
Cdd:cd17652 83 ARPALLL------TTPD--------------------------------------------------------------- 93
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 226 vpvqgcDIAFLQYTGGTTGISKGAMLTHSNI--LANVEQMnlwmdvAFRNKGKPKALNFvcALPLYHIFALTvnamIGMK 303
Cdd:cd17652 94 ------NLAYVIYTSGSTGRPKGVVVTHRGLanLAAAQIA------AFDVGPGSRVLQF--ASPSFDASVWE----LLMA 155
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 304 L--GARNILIPNPRDIPS--FVKELKKYPV-HIF--PG-LNTLFNGlmNNPDFQTLdfkpliltLGGGMAVQRPVAERWQ 375
Cdd:cd17652 156 LlaGATLVLAPAEELLPGepLADLLREHRItHVTlpPAaLAALPPD--DLPDLRTL--------VVAGEACPAELVDRWA 225
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 376 hmTGCHITEGYGLSETSPVACANALDATEFTGTIGLPMPSTDVVIRDDDGSDLLLGKVGEICVRGPQVMKGYWNRPEETA 455
Cdd:cd17652 226 --PGRRMINAYGPTETTVCATMAGPLPGGGVPPIGRPVPGTRVYVLDARLRPVPPGVPGELYIAGAGLARGYLNRPGLTA 303
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 456 RAIMADGF-------FRTGDMGFMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVAAEHPGVLESAAVGIPNEHSGEVVK 528
Cdd:cd17652 304 ERFVADPFgapgsrmYRTGDLARWRADGQLEFLGRADDQVKIRGFRIELGEVEAALTEHPGVAEAVVVVRDDRPGDKRLV 383
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|..
gi 479816261 529 LYVVRRDPE-LTEDEVKAFCAERLTNYKRPREVEFRESLPKSNVGKILRREL 579
Cdd:cd17652 384 AYVVPAPGAaPTAAELRAHLAERLPGYMVPAAFVVLDALPLTPNGKLDRRAL 435
|
|
| PRK13383 |
PRK13383 |
acyl-CoA synthetase; Provisional |
76-579 |
2.74e-29 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 139531 [Multi-domain] Cd Length: 516 Bit Score: 121.64 E-value: 2.74e-29
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 76 ISYKELDDHSRALAAWLQSRGLVKGDRVAIMMPNILQYPITFTAVLRAGFVVVNVNPLYTPRELEHQLNDAGAKALVVLE 155
Cdd:PRK13383 61 LSYRELQRATESLARRLTRDGVAPGRAVGVMCRNGRGFVTAVFAVGLLGADVVPISTEFRSDALAAALRAHHISTVVADN 140
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 156 NFATTVEKALPSINVPNILVASMGDMLGfkghivnlvvrrvkkmVPAWNIPGHVrfkdalaqgraksfnpvpvqgcdiaf 235
Cdd:PRK13383 141 EFAERIAGADDAVAVIDPATAGAEESGG----------------RPAVAAPGRI-------------------------- 178
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 236 LQYTGGTTGISKG---AMLTHSNIlanveqmNLWMDVAFRNKGKPKAlNFVCALPLYHIFALTVnAMIGMKLGArNILIP 312
Cdd:PRK13383 179 VLLTSGTTGKPKGvprAPQLRSAV-------GVWVTILDRTRLRTGS-RISVAMPMFHGLGLGM-LMLTIALGG-TVLTH 248
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 313 NPRDIPSFVKELKKYPVHIFPGLNTLFNGLMNNPDfQTLDFKPLI---LTLGGGMAVQRPVAERWQHMTGCHITEGYGLS 389
Cdd:PRK13383 249 RHFDAEAALAQASLHRADAFTAVPVVLARILELPP-RVRARNPLPqlrVVMSSGDRLDPTLGQRFMDTYGDILYNGYGST 327
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 390 ETSPVACANALDATEFTGTIGLPMPSTDVVIRDDDGSDLLLGKVGEICVRGPQVMKGYwnrPEETARAIMaDGFFRTGDM 469
Cdd:PRK13383 328 EVGIGALATPADLRDAPETVGKPVAGCPVRILDRNNRPVGPRVTGRIFVGGELAGTRY---TDGGGKAVV-DGMTSTGDM 403
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 470 GFMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVAAEHPGVLESAAVGIPNEHSGEVVKLYVVRR-DPELTEDEVKAFCA 548
Cdd:PRK13383 404 GYLDNAGRLFIVGREDDMIISGGENVYPRAVENALAAHPAVADNAVIGVPDERFGHRLAAFVVLHpGSGVDAAQLRDYLK 483
|
490 500 510
....*....|....*....|....*....|.
gi 479816261 549 ERLTNYKRPREVEFRESLPKSNVGKILRREL 579
Cdd:PRK13383 484 DRVSRFEQPRDINIVSSIPRNPTGKVLRKEL 514
|
|
| LC_FACS_bac1 |
cd17641 |
bacterial long-chain fatty acid CoA synthetase; The members of this family are bacterial ... |
74-517 |
4.11e-29 |
|
bacterial long-chain fatty acid CoA synthetase; The members of this family are bacterial long-chain fatty acid CoA synthetase, most of which are as yet uncharacterized. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.
Pssm-ID: 341296 [Multi-domain] Cd Length: 569 Bit Score: 121.76 E-value: 4.11e-29
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 74 KDISYKELDDHSRALAAWLQSRGLVKGDRVAIMMPNILQYPITFTAVLRAGFVVVNVNPLYTPRELEHQLNDAGAKALVV 153
Cdd:cd17641 10 QEFTWADYADRVRAFALGLLALGVGRGDVVAILGDNRPEWVWAELAAQAIGALSLGIYQDSMAEEVAYLLNYTGARVVIA 89
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 154 LENfaTTVEKALPsinvpnilvasmgdmLGFKGHIVNLVVRRVKKMVPAWNIPGHVRFKDALAQGRA-KSFNPVPVQ--- 229
Cdd:cd17641 90 EDE--EQVDKLLE---------------IADRIPSVRYVIYCDPRGMRKYDDPRLISFEDVVALGRAlDRRDPGLYErev 152
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 230 ----GCDIAFLQYTGGTTGISKGAMLTHSNILanvEQMNLWMDVAFRNKGKpkalNFVCALPLYHI--FALTVnamiGMK 303
Cdd:cd17641 153 aagkGEDVAVLCTTSGTTGKPKLAMLSHGNFL---GHCAAYLAADPLGPGD----EYVSVLPLPWIgeQMYSV----GQA 221
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 304 LGARNI---------LIPNPRDI-PSFV----------------KELKKYPVHIF----------PGLNTLFNGLMNNPD 347
Cdd:cd17641 222 LVCGFIvnfpeepetMMEDLREIgPTFVllpprvwegiaadvraRMMDATPFKRFmfelgmklglRALDRGKRGRPVSLW 301
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 348 -------FQTLDFKPLILTLG---------GGMAVQrPVAERWQHMTGCHITEGYGLSETSPVACANALDATEFTgTIGL 411
Cdd:cd17641 302 lrlaswlADALLFRPLRDRLGfsrlrsaatGGAALG-PDTFRFFHAIGVPLKQLYGQTELAGAYTVHRDGDVDPD-TVGV 379
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 412 PMPSTDVVIRDddgsdlllgkVGEICVRGPQVMKGYWNRPEETARAIMADGFFRTGDMGFMDERGYTKIVDRKKD-MILV 490
Cdd:cd17641 380 PFPGTEVRIDE----------VGEILVRSPGVFVGYYKNPEATAEDFDEDGWLHTGDAGYFKENGHLVVIDRAKDvGTTS 449
|
490 500
....*....|....*....|....*..
gi 479816261 491 SGFNVYPNEIEEVAAEHPGVLESAAVG 517
Cdd:cd17641 450 DGTRFSPQFIENKLKFSPYIAEAVVLG 476
|
|
| A_NRPS_PpsD_like |
cd17650 |
similar to adenylation domain of plipastatin synthase (PpsD); This family of the adenylation ... |
65-579 |
7.22e-29 |
|
similar to adenylation domain of plipastatin synthase (PpsD); This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes bacitracin synthetase 1 (BacA) in Bacillus licheniformis, tyrocidine synthetase in Brevibacillus brevis, plipastatin synthase (PpsD, an important antifungal protein) in Bacillus subtilis and mannopeptimycin peptide synthetase (MppB) in Streptomyces hygroscopicus. Plipastatin has strong fungitoxic activity and is involved in inhibition of phospholipase A2 and biofilm formation. Bacitracin, a mixture of related cyclic peptides, is used as a polypeptide antibiotic while function of tyrocidine is thought to be regulation of sporulation. MppB is involved in biosynthetic pathway of mannopeptimycin, a novel class of mannosylated lipoglycopeptides. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341305 [Multi-domain] Cd Length: 447 Bit Score: 119.49 E-value: 7.22e-29
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 65 NSPAFTCMGKDISYKELDDHSRALAAWLQSRGLVKGDRVAIMMPNILQYPITFTAVLRAGFVVVNVNPLYTPRELEHQLN 144
Cdd:cd17650 2 DAIAVSDATRQLTYRELNERANQLARTLRGLGVAPGSVVGVCADRSLDAIVGLLAVLKAGGAYVPIDPDYPAERLQYMLE 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 145 DAGAKALVVLenfattvekalpsinvpnilvasmgdmlgfkghivnlvvrrvkkmvpawniPGhvrfkdalaqgraksfn 224
Cdd:cd17650 82 DSGAKLLLTQ---------------------------------------------------PE----------------- 93
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 225 pvpvqgcDIAFLQYTGGTTGISKGAMLTHSNILAnveqmnlwMDVAFRNKgkpkalnfvcalplYHIFALTVNAM----- 299
Cdd:cd17650 94 -------DLAYVIYTSGTTGKPKGVMVEHRNVAH--------AAHAWRRE--------------YELDSFPVRLLqmasf 144
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 300 --------IGMKLGARNILIPNPRDI----PSFVKELKKYPVHIFPGLNTLFNGLMNNPDFQTLDFKPL-ILTLGGGMAV 366
Cdd:cd17650 145 sfdvfagdFARSLLNGGTLVICPDEVkldpAALYDLILKSRITLMESTPALIRPVMAYVYRNGLDLSAMrLLIVGSDGCK 224
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 367 QR----PVAERWQHMtgcHITEGYGLSETSpvacanaLDATEFTGT-----------IGLPMPSTDVVIRDDDGSDLLLG 431
Cdd:cd17650 225 AQdfktLAARFGQGM---RIINSYGVTEAT-------IDSTYYEEGrdplgdsanvpIGRPLPNTAMYVLDERLQPQPVG 294
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 432 KVGEICVRGPQVMKGYWNRPEETARAIMADGF------FRTGDMGFMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVAA 505
Cdd:cd17650 295 VAGELYIGGAGVARGYLNRPELTAERFVENPFapgermYRTGDLARWRADGNVELLGRVDHQVKIRGFRIELGEIESQLA 374
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 479816261 506 EHPGVLEsAAVGIPNEHSGEV-VKLYVVRRDpELTEDEVKAFCAERLTNYKRPREVEFRESLPKSNVGKILRREL 579
Cdd:cd17650 375 RHPAIDE-AVVAVREDKGGEArLCAYVVAAA-TLNTAELRAFLAKELPSYMIPSYYVQLDALPLTPNGKVDRRAL 447
|
|
| PRK05620 |
PRK05620 |
long-chain fatty-acid--CoA ligase; |
76-580 |
1.07e-28 |
|
long-chain fatty-acid--CoA ligase;
Pssm-ID: 180167 [Multi-domain] Cd Length: 576 Bit Score: 120.66 E-value: 1.07e-28
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 76 ISYKELDDHSRALAAWLQSR-GLVKGDRVAIMMPNILQYPITFTAVLRAGFVVVNVNPLYTPRELEHQLNDAGAKALVVL 154
Cdd:PRK05620 39 TTFAAIGARAAALAHALHDElGITGDQRVGSMMYNCAEHLEVLFAVACMGAVFNPLNKQLMNDQIVHIINHAEDEVIVAD 118
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 155 ENFATTVEKALPSI-NVPNILVASMGDMlgfkghivnlvvrrvkkMVPAWNIPGHVRFKD--ALAQGRAKSFNPVPVQGC 231
Cdd:PRK05620 119 PRLAEQLGEILKECpCVRAVVFIGPSDA-----------------DSAAAHMPEGIKVYSyeALLDGRSTVYDWPELDET 181
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 232 DIAFLQYTGGTTGISKGAMLTHSNILANVEQMNLWMDVAFRNkGKPkalnFVCALPLYHIFALTVNAMIGMKlGARNILI 311
Cdd:PRK05620 182 TAAAICYSTGTTGAPKGVVYSHRSLYLQSLSLRTTDSLAVTH-GES----FLCCVPIYHVLSWGVPLAAFMS-GTPLVFP 255
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 312 PNPRDIPSFVKELKKYPVHIFPGLNTLFNGLM-----NNPDFQTLDfkpliLTLGGGMAVQRPVAERWQHMTGCHITEGY 386
Cdd:PRK05620 256 GPDLSAPTLAKIIATAMPRVAHGVPTLWIQLMvhylkNPPERMSLQ-----EIYVGGSAVPPILIKAWEERYGVDVVHVW 330
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 387 GLSETSPVacanaldateftGTIGLPmPS-----TDVVIRDDDG-----------SDlllGKV--------GEICVRGPQ 442
Cdd:PRK05620 331 GMTETSPV------------GTVARP-PSgvsgeARWAYRVSQGrfpasleyrivND---GQVmestdrneGEIQVRGNW 394
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 443 VMKGYWNRP----------------EETARAIMADGFFRTGDMGFMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVAAE 506
Cdd:PRK05620 395 VTASYYHSPteegggaastfrgedvEDANDRFTADGWLRTGDVGSVTRDGFLTIHDRARDVIRSGGEWIYSAQLENYIMA 474
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 479816261 507 HPGVLESAAVGIPNEHSGE----VVKLYVVRRDPELTEDEVKAFCAERLTNYKRPREVEFRESLPKSNVGKILRRELR 580
Cdd:PRK05620 475 APEVVECAVIGYPDDKWGErplaVTVLAPGIEPTRETAERLRDQLRDRLPNWMLPEYWTFVDEIDKTSVGKFDKKDLR 552
|
|
| FATP_FACS |
cd05940 |
Fatty acid transport proteins (FATP) play dual roles as fatty acid transporters and its ... |
73-580 |
1.36e-28 |
|
Fatty acid transport proteins (FATP) play dual roles as fatty acid transporters and its activation enzymes; Fatty acid transport protein (FATP) transports long-chain or very-long-chain fatty acids across the plasma membrane. FATPs also have fatty acid CoA synthetase activity, thus playing dual roles as fatty acid transporters and its activation enzymes. At least five copies of FATPs are identified in mammalian cells. This family also includes prokaryotic FATPs. FATPs are the key players in the trafficking of exogenous fatty acids into the cell and in intracellular fatty acid homeostasis.
Pssm-ID: 341263 [Multi-domain] Cd Length: 449 Bit Score: 118.61 E-value: 1.36e-28
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 73 GKDISYKELDDHSRALAAWLQSRGLVKGDRVAIMMPNILQYPITFTAVLRAGFVVVNVNPLYTPRELEHQLNDAGAKALV 152
Cdd:cd05940 1 DEALTYAELDAMANRYARWLKSLGLKPGDVVALFMENRPEYVLLWLGLVKIGAVAALINYNLRGESLAHCLNVSSAKHLV 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 153 VlenfattvekalpsinvpnilvasmgdmlgfkghivnlvvrrvkkmvpawnipghvrfkdalaqgraksfnpvpvqgcD 232
Cdd:cd05940 81 V------------------------------------------------------------------------------D 82
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 233 IAFLQYTGGTTGISKGAMLTHSNILanveQMNLWmdVAFRNKGKPKALNFVCaLPLYHIFALTVnAMIGMKLGARNILIP 312
Cdd:cd05940 83 AALYIYTSGTTGLPKAAIISHRRAW----RGGAF--FAGSGGALPSDVLYTC-LPLYHSTALIV-GWSACLASGATLVIR 154
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 313 NPRDIPSFVKELKKYPVHIFPGLNTLFNGLMNNPDFQTLDFKPLILTLGGGMavqRPvaERWQHMTG----CHITEGYGL 388
Cdd:cd05940 155 KKFSASNFWDDIRKYQATIFQYIGELCRYLLNQPPKPTERKHKVRMIFGNGL---RP--DIWEEFKErfgvPRIAEFYAA 229
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 389 SE--TSPVACANaldateFTGTIG-----LPMPSTDVVIRDDDGSDLLL------------GKVGE-ICVRGP-QVMKGY 447
Cdd:cd05940 230 TEgnSGFINFFG------KPGAIGrnpslLRKVAPLALVKYDLESGEPIrdaegrcikvprGEPGLlISRINPlEPFDGY 303
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 448 WNrPEETARAIMADGF------FRTGDMGFMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVAAEHPGVLESAAVG--IP 519
Cdd:cd05940 304 TD-PAATEKKILRDVFkkgdawFNTGDLMRLDGEGFWYFVDRLGDTFRWKGENVSTTEVAAVLGAFPGVEEANVYGvqVP 382
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....
gi 479816261 520 NeHSGEVVKLYVVRRDPEltEDEVKAFCA---ERLTNYKRPREVEFRESLPKSNVGKILRRELR 580
Cdd:cd05940 383 G-TDGRAGMAAIVLQPNE--EFDLSALAAhleKNLPGYARPLFLRLQPEMEITGTFKQQKVDLR 443
|
|
| PRK12316 |
PRK12316 |
peptide synthase; Provisional |
66-579 |
1.40e-28 |
|
peptide synthase; Provisional
Pssm-ID: 237054 [Multi-domain] Cd Length: 5163 Bit Score: 121.99 E-value: 1.40e-28
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 66 SPAFTCMGKDISYKELDDHSRALAAWLQSRGLVKGDRVAIMMPNILQYPITFTAVLRAGFVVVNVNPLYTPRELEHQLND 145
Cdd:PRK12316 527 APALAFGEETLDYAELNRRANRLAHALIERGVGPDVLVGVAMERSIEMVVALLAILKAGGAYVPLDPEYPAERLAYMLED 606
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 146 AGAkALVVLENFattvekalpsinvpnilvasMGDMLGFKGHIVNLVVRRvkkmvPAWNIPGHvrfkdalaqgraKSFNP 225
Cdd:PRK12316 607 SGV-QLLLSQSH--------------------LGRKLPLAAGVQVLDLDR-----PAAWLEGY------------SEENP 648
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 226 -VPVQGCDIAFLQYTGGTTGISKGAMLTHSNILANVEqmnlWMDVAFrnkGKPKALNFVCALPLYhiFALTVNAMIG-MK 303
Cdd:PRK12316 649 gTELNPENLAYVIYTSGSTGKPKGAGNRHRALSNRLC----WMQQAY---GLGVGDTVLQKTPFS--FDVSVWEFFWpLM 719
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 304 LGARNILIP--NPRDIPSFVKELKKYPVHIFPGLNTLFNGLMNNPDFQTLDfkPL-ILTLGG----GMAVQRPVAERWQh 376
Cdd:PRK12316 720 SGARLVVAApgDHRDPAKLVELINREGVDTLHFVPSMLQAFLQDEDVASCT--SLrRIVCSGealpADAQEQVFAKLPQ- 796
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 377 mtgCHITEGYGLSETS-PVACANALDATEFTGTIGLPMPSTDVVIRDDDGSDLLLGKVGEICVRGPQVMKGYWNRPEETA 455
Cdd:PRK12316 797 ---AGLYNLYGPTEAAiDVTHWTCVEEGGDSVPIGRPIANLACYILDANLEPVPVGVLGELYLAGRGLARGYHGRPGLTA 873
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 456 RAIMADGF------FRTGDMGFMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVAAEHPGVLESAAVGIpnehSGEVVKL 529
Cdd:PRK12316 874 ERFVPSPFvagermYRTGDLARYRADGVIEYAGRIDHQVKLRGLRIELGEIEARLLEHPWVREAAVLAV----DGKQLVG 949
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|.
gi 479816261 530 YVVRRDPELTEDEV-KAFCAERLTNYKRPREVEFRESLPKSNVGKILRREL 579
Cdd:PRK12316 950 YVVLESEGGDWREAlKAHLAASLPEYMVPAQWLALERLPLTPNGKLDRKAL 1000
|
|
| A_NRPS_LgrA-like |
cd17645 |
adenylation (A) domain of linear gramicidin synthetase (LgrA) and similar proteins; This ... |
67-579 |
2.25e-28 |
|
adenylation (A) domain of linear gramicidin synthetase (LgrA) and similar proteins; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes linear gramicidin synthetase (LgrA) in Brevibacillus brevis. LgrA has a formylation domain fused to the N-terminal end that formylates its substrate for linear gramicidin synthesis to proceed. This formyl group is essential for the clinically important antibacterial activity of gramicidin by enabling head-to-head gramicidin dimers to make a beta-helical pore in gram-positive bacterial membranes, allowing free passage of monovalent cations, destroying the ion gradient and killing bacteria. This family also includes bacitracin synthetase 1 (known as ATP-dependent cysteine adenylase or BA1); it activates cysteine, incorporates two D-amino acids, releases and cyclizes the mature bacitracin, an antibiotic that is a mixture of related cyclic peptides that disrupt gram positive bacteria by interfering with cell wall and peptidoglycan synthesis. Also included is surfactin synthetase which activates and polymerizes the amino acids Leu, Glu, Asp, and Val to form the antibiotic surfactin.
Pssm-ID: 341300 [Multi-domain] Cd Length: 440 Bit Score: 118.04 E-value: 2.25e-28
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 67 PAFTCMGKDISYKELDDHSRALAAWLQSRGLVKGDRVAIMMPNILQYPITFTAVLRAGFVVVNVNPLYTPRELEHQLNDA 146
Cdd:cd17645 15 VAVVDRGQSLTYKQLNEKANQLARHLRGKGVKPDDQVGIMLDKSLDMIAAILGVLKAGGAYVPIDPDYPGERIAYMLADS 94
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 147 GAKALVVlenfattvekalpsinvpnilvasmgdmlgfkghivnlvvrrvkkmvpawnipghvrfkdalaqgraksfnpv 226
Cdd:cd17645 95 SAKILLT------------------------------------------------------------------------- 101
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 227 pvQGCDIAFLQYTGGTTGISKGAMLTHSNILanveqmNL--WMDVAFRNKGKPKALNFvcALPLYHIFALTVNAMIgmKL 304
Cdd:cd17645 102 --NPDDLAYVIYTSGSTGLPKGVMIEHHNLV------NLceWHRPYFGVTPADKSLVY--ASFSFDASAWEIFPHL--TA 169
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 305 GARNILIPNPRDIPsfVKELKKYPVHIFPGLNTLFNGLMNNpdFQTLDFKPLILTLGGGMAVQRPVAERWQhmtgchITE 384
Cdd:cd17645 170 GAALHVVPSERRLD--LDALNDYFNQEGITISFLPTGAAEQ--FMQLDNQSLRVLLTGGDKLKKIERKGYK------LVN 239
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 385 GYGLSETSPVACANALDATEFTGTIGLPMPSTDVVIRDDDGSDLLLGKVGEICVRGPQVMKGYWNRPEETARAIMADGF- 463
Cdd:cd17645 240 NYGPTENTVVATSFEIDKPYANIPIGKPIDNTRVYILDEALQLQPIGVAGELCIAGEGLARGYLNRPELTAEKFIVHPFv 319
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 464 -----FRTGDMGFMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVAAEHPGVLESAAVGIPNEHSGEVVKLYVVRRDpEL 538
Cdd:cd17645 320 pgermYRTGDLAKFLPDGNIEFLGRLDQQVKIRGYRIEPGEIEPFLMNHPLIELAAVLAKEDADGRKYLVAYVTAPE-EI 398
|
490 500 510 520
....*....|....*....|....*....|....*....|.
gi 479816261 539 TEDEVKAFCAERLTNYKRPREVEFRESLPKSNVGKILRREL 579
Cdd:cd17645 399 PHEELREWLKNDLPDYMIPTYFVHLKALPLTANGKVDRKAL 439
|
|
| PRK05691 |
PRK05691 |
peptide synthase; Validated |
76-580 |
2.88e-28 |
|
peptide synthase; Validated
Pssm-ID: 235564 [Multi-domain] Cd Length: 4334 Bit Score: 121.04 E-value: 2.88e-28
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 76 ISYKELDDHSRALAAWLQSRGlVKGDRVAIMMPNILQYPITFTAVLRAGFVVVnvnPLYTPREL--EHQlndagakalvv 153
Cdd:PRK05691 41 LSYRDLDLRARTIAAALQARA-SFGDRAVLLFPSGPDYVAAFFGCLYAGVIAV---PAYPPESArrHHQ----------- 105
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 154 lENFATTVEKALPSInvpnIL-VASMGDMLGFKGHIVnlvvrrvkkmvpAWNIPGHVRFkDALAQGRAKSFNPVPVQGCD 232
Cdd:PRK05691 106 -ERLLSIIADAEPRL----LLtVADLRDSLLQMEELA------------AANAPELLCV-DTLDPALAEAWQEPALQPDD 167
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 233 IAFLQYTGGTTGISKGAMLTHSNILANvEQMnlwMDVAFRNKGKPKALnFVCALPLYHIFALTVNAMIGMKLGARNILIP 312
Cdd:PRK05691 168 IAFLQYTSGSTALPKGVQVSHGNLVAN-EQL---IRHGFGIDLNPDDV-IVSWLPLYHDMGLIGGLLQPIFSGVPCVLMS 242
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 313 nprdiPSFVKElkkYPVHIFPGLNTLFNGLMNNPDF--------------QTLDFKPLILTLGGGMAVQRPVAERW-QHM 377
Cdd:PRK05691 243 -----PAYFLE---RPLRWLEAISEYGGTISGGPDFayrlcservsesalERLDLSRWRVAYSGSEPIRQDSLERFaEKF 314
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 378 TGCHITE-----GYGLSE-TSPVA--------CANALDATEF--------TGTI----GLPMPSTDVVIRDDDGSDLLL- 430
Cdd:PRK05691 315 AACGFDPdsffaSYGLAEaTLFVSggrrgqgiPALELDAEALarnraepgTGSVlmscGRSQPGHAVLIVDPQSLEVLGd 394
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 431 GKVGEICVRGPQVMKGYWNRPEETARA-IMADG--FFRTGDMGFMDErGYTKIVDRKKDMILVSGFNVYPNEIE-----E 502
Cdd:PRK05691 395 NRVGEIWASGPSIAHGYWRNPEASAKTfVEHDGrtWLRTGDLGFLRD-GELFVTGRLKDMLIVRGHNLYPQDIEktverE 473
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 503 VAAEHPGVLESAAV--------GIPNEHSGEVVKLYvvrrDPELTEDEVKAFCAErlTNYKRPREVEFRE--SLPKSNVG 572
Cdd:PRK05691 474 VEVVRKGRVAAFAVnhqgeegiGIAAEISRSVQKIL----PPQALIKSIRQAVAE--ACQEAPSVVLLLNpgALPKTSSG 547
|
....*...
gi 479816261 573 KILRRELR 580
Cdd:PRK05691 548 KLQRSACR 555
|
|
| PRK07824 |
PRK07824 |
o-succinylbenzoate--CoA ligase; |
218-580 |
1.32e-27 |
|
o-succinylbenzoate--CoA ligase;
Pssm-ID: 236108 [Multi-domain] Cd Length: 358 Bit Score: 114.37 E-value: 1.32e-27
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 218 GRAKSFNPVPVQGC------------------DIAFLQYTGGTTGISKGAMLTHSNILANVeqmnlwmDVAFRNKGKPKa 279
Cdd:PRK07824 4 GRAPALLPVPAQDErraallrdalrvgepiddDVALVVATSGTTGTPKGAMLTAAALTASA-------DATHDRLGGPG- 75
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 280 lNFVCALPLYHIfaltvnamIGMKLGARNIL---------IP---NPRDIPSFVKELK---KY----PVHIFPGLNTLFN 340
Cdd:PRK07824 76 -QWLLALPAHHI--------AGLQVLVRSVIagsepveldVSagfDPTALPRAVAELGggrRYtslvPMQLAKALDDPAA 146
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 341 glmnnpdfqTLDFKPLILTLGGGMAVQRPVAERWQHMtGCHITEGYGLSETSpvacanaldatefTGTI--GLPMPSTDV 418
Cdd:PRK07824 147 ---------TAALAELDAVLVGGGPAPAPVLDAAAAA-GINVVRTYGMSETS-------------GGCVydGVPLDGVRV 203
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 419 VIRDddgsdlllgkvGEICVRGPQVMKGYWNRPEETARAimADGFFRTGDMGFMDErGYTKIVDRKKDMILVSGFNVYPN 498
Cdd:PRK07824 204 RVED-----------GRIALGGPTLAKGYRNPVDPDPFA--EPGWFRTDDLGALDD-GVLTVLGRADDAISTGGLTVLPQ 269
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 499 EIEEVAAEHPGVLESAAVGIPNEHSGE-VVKLYVVRRDPELTEDEVKAFCAERLTNYKRPREVEFRESLPKSNVGKILRR 577
Cdd:PRK07824 270 VVEAALATHPAVADCAVFGLPDDRLGQrVVAAVVGDGGPAPTLEALRAHVARTLDRTAAPRELHVVDELPRRGIGKVDRR 349
|
...
gi 479816261 578 ELR 580
Cdd:PRK07824 350 ALV 352
|
|
| PRK12467 |
PRK12467 |
peptide synthase; Provisional |
34-581 |
2.53e-27 |
|
peptide synthase; Provisional
Pssm-ID: 237108 [Multi-domain] Cd Length: 3956 Bit Score: 117.95 E-value: 2.53e-27
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 34 KSYPAGVPHDINLSAATSISD-----MISASCRQFANSPAFTCMGKDISYKELDDHSRALAAWLQSRGlVKGDR-VAIMM 107
Cdd:PRK12467 3074 AHERRQVLHAWNATAAAYPSErlvhqLIEAQVARTPEAPALVFGDQQLSYAELNRRANRLAHRLIAIG-VGPDVlVGVAV 3152
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 108 PNILQYPITFTAVLRAGFVVVNVNPLYtPRE-LEHQLNDAGAKALV----VLENfattvekaLPSINVPNILVASMGDML 182
Cdd:PRK12467 3153 ERSVEMIVALLAVLKAGGAYVPLDPEY-PRErLAYMIEDSGVKLLLtqahLLEQ--------LPAPAGDTALTLDRLDLN 3223
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 183 GFKGHivnlvvrrvkkmvpawnipghvrfkdalaqgraksfNPVP-VQGCDIAFLQYTGGTTGISKGAMLTHSNiLANVE 261
Cdd:PRK12467 3224 GYSEN------------------------------------NPSTrVMGENLAYVIYTSGSTGKPKGVGVRHGA-LANHL 3266
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 262 QmnlWMDVAFRNKGKPKALNFVCalplyHIFALTVNAMIGMKL-GARNILIPNP-RDIPSFVKELKKYPVHIFPGLNTLF 339
Cdd:PRK12467 3267 C---WIAEAYELDANDRVLLFMS-----FSFDGAQERFLWTLIcGGCLVVRDNDlWDPEELWQAIHAHRISIACFPPAYL 3338
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 340 NGLMNNPDFQtlDFKPLILTLGGGMAVQRPVAERWQ-HMTGCHITEGYGLSET--SPVACANALDATEFTGT--IGLPMP 414
Cdd:PRK12467 3339 QQFAEDAGGA--DCASLDIYVFGGEAVPPAAFEQVKrKLKPRGLTNGYGPTEAvvTVTLWKCGGDAVCEAPYapIGRPVA 3416
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 415 STDVVIRDDDGSDLLLGKVGEICVRGPQVMKGYWNRPEETARAIMADGF-------FRTGDMGFMDERGYTKIVDRKKDM 487
Cdd:PRK12467 3417 GRSIYVLDGQLNPVPVGVAGELYIGGVGLARGYHQRPSLTAERFVADPFsgsggrlYRTGDLARYRADGVIEYLGRIDHQ 3496
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 488 ILVSGFNVYPNEIEEVAAEHPGVLESAAVGIPNEHSGEVVKlYVVRRDP-ELTEDEVKAFCAERLTNYKRPREVEFRESL 566
Cdd:PRK12467 3497 VKIRGFRIELGEIEARLLQHPSVREAVVLARDGAGGKQLVA-YVVPADPqGDWRETLRDHLAASLPDYMVPAQLLVLAAM 3575
|
570
....*....|....*
gi 479816261 567 PKSNVGKILRRELRD 581
Cdd:PRK12467 3576 PLGPNGKVDRKALPD 3590
|
|
| FACL_like_5 |
cd05924 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
232-573 |
2.69e-27 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341248 [Multi-domain] Cd Length: 364 Bit Score: 113.63 E-value: 2.69e-27
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 232 DIAFLQYTGGTTGISKGAMLTHSNI---------LANVEQmnLWMDVAFRNKGKPKALNFVCALPLYHiFALTVNAMIGM 302
Cdd:cd05924 4 DDLYILYTGGTTGMPKGVMWRQEDIfrmlmggadFGTGEF--TPSEDAHKAAAAAAGTVMFPAPPLMH-GTGSWTAFGGL 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 303 kLGARNILIPNPR-DIPSFVKELKKYPVhifpGLNTLFNGLMNNP------DFQTLDFKPLILTLGGGMAVQRPVAERWQ 375
Cdd:cd05924 81 -LGGQTVVLPDDRfDPEEVWRTIEKHKV----TSMTIVGDAMARPlidalrDAGPYDLSSLFAISSGGALLSPEVKQGLL 155
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 376 -HMTGCHITEGYGLSETSPVACANALDATEFTGTIGLPMPSTDVVirDDDGSDLLLGK--VGEICVRGpQVMKGYWNRPE 452
Cdd:cd05924 156 eLVPNITLVDAFGSSETGFTGSGHSAGSGPETGPFTRANPDTVVL--DDDGRVVPPGSggVGWIARRG-HIPLGYYGDEA 232
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 453 ETARAIMADGFFR---TGDMGFMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVAAEHPGVLESAAVGIPNEHSG-EVVK 528
Cdd:cd05924 233 KTAETFPEVDGVRyavPGDRATVEADGTVTLLGRGSVCINTGGEKVFPEEVEEALKSHPAVYDVLVVGRPDERWGqEVVA 312
|
330 340 350 360
....*....|....*....|....*....|....*....|....*
gi 479816261 529 LYVVRRDPELTEDEVKAFCAERLTNYKRPREVEFRESLPKSNVGK 573
Cdd:cd05924 313 VVQLREGAGVDLEELREHCRTRIARYKLPKQVVFVDEIERSPAGK 357
|
|
| PRK05857 |
PRK05857 |
fatty acid--CoA ligase; |
337-579 |
3.66e-27 |
|
fatty acid--CoA ligase;
Pssm-ID: 180293 [Multi-domain] Cd Length: 540 Bit Score: 115.49 E-value: 3.66e-27
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 337 TLFNGLMNNPDFQTLDFKPL-ILTLGGGMAVQRPVaeRWQHMTGCHITEGYGLSETSPVACANALDATEFT----GTIGL 411
Cdd:PRK05857 269 TLLSKLVSELKSANATVPSLrLVGYGGSRAIAADV--RFIEATGVRTAQVYGLSETGCTALCLPTDDGSIVkieaGAVGR 346
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 412 PMPSTDVVIRDDDGSDLLLGKVGE------ICVRGPQVMKGYWNRPEETaRAIMADGFFRTGDMGFMDERGYTKIVDRKK 485
Cdd:PRK05857 347 PYPGVDVYLAATDGIGPTAPGAGPsasfgtLWIKSPANMLGYWNNPERT-AEVLIDGWVNTGDLLERREDGFFYIKGRSS 425
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 486 DMILVSGFNVYPNEIEEVAAEHPGVLESAAVGIPNEHSGEVVKLYVVrRDPELTEDEVKAFCAERLTNYKRPREVEFRES 565
Cdd:PRK05857 426 EMIICGGVNIAPDEVDRIAEGVSGVREAACYEIPDEEFGALVGLAVV-ASAELDESAARALKHTIAARFRRESEPMARPS 504
|
250 260
....*....|....*....|.
gi 479816261 566 -------LPKSNVGKILRREL 579
Cdd:PRK05857 505 tivivtdIPRTQSGKVMRASL 525
|
|
| PRK13388 |
PRK13388 |
acyl-CoA synthetase; Provisional |
49-581 |
4.12e-27 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 237374 [Multi-domain] Cd Length: 540 Bit Score: 115.51 E-value: 4.12e-27
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 49 ATSISDMISAscRQFANSPAFTCMGKDISYKELDDHSRALAAWLqsRGLVKGDR---VAIMMPNILQYPITFTAVLRAGF 125
Cdd:PRK13388 2 RDTIAQLLRD--RAGDDTIAVRYGDRTWTWREVLAEAAARAAAL--IALADPDRplhVGVLLGNTPEMLFWLAAAALGGY 77
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 126 VVVNVNPLYTPRELEHQLNDAGAKALVVlenfATTVEKALPSINVPNILVASMGDmlgfkghivnlvvrrvkkmvPAWni 205
Cdd:PRK13388 78 VLVGLNTTRRGAALAADIRRADCQLLVT----DAEHRPLLDGLDLPGVRVLDVDT--------------------PAY-- 131
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 206 pghvrfKDALAqgRAKSFNPVPVQGCDIAFLQY-TGGTTGISKGAMLTHSNIL----ANVEQMNLWM-DVAFrnkgkpka 279
Cdd:PRK13388 132 ------AELVA--AAGALTPHREVDAMDPFMLIfTSGTTGAPKAVRCSHGRLAfagrALTERFGLTRdDVCY-------- 195
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 280 lnfvCALPLYHIFAltVNAMIGMKLGARNILIPNPRDIPS-FVKELKKYPVhifpglnTLFN--G-----LMNNPDFQTL 351
Cdd:PRK13388 196 ----VSMPLFHSNA--VMAGWAPAVASGAAVALPAKFSASgFLDDVRRYGA-------TYFNyvGkplayILATPERPDD 262
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 352 DFKPLILTLGGgMAVQRPVAErWQHMTGCHITEGYGLSETspvACANALDATEFTGTIGLPMPstDVVIRD--------- 422
Cdd:PRK13388 263 ADNPLRVAFGN-EASPRDIAE-FSRRFGCQVEDGYGSSEG---AVIVVREPGTPPGSIGRGAP--GVAIYNpetltecav 335
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 423 ---DDGSDLLLGK--VGEICVR-GPQVMKGYWNRPEETARAiMADGFFRTGDMGFMDERGYTKIVDRKKDMILVSGFNVY 496
Cdd:PRK13388 336 arfDAHGALLNADeaIGELVNTaGAGFFEGYYNNPEATAER-MRHGMYWSGDLAYRDADGWIYFAGRTADWMRVDGENLS 414
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 497 PNEIEEVAAEHPGVLESAAVGIPNEHSGEVVKLYVVRRDPE-LTEDEVKAFCAER--LTNYKRPREVEFRESLPKSNVGK 573
Cdd:PRK13388 415 AAPIERILLRHPAINRVAVYAVPDERVGDQVMAALVLRDGAtFDPDAFAAFLAAQpdLGTKAWPRYVRIAADLPSTATNK 494
|
....*...
gi 479816261 574 ILRRELRD 581
Cdd:PRK13388 495 VLKRELIA 502
|
|
| PRK12582 |
PRK12582 |
acyl-CoA synthetase; Provisional |
76-475 |
4.30e-27 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 237144 [Multi-domain] Cd Length: 624 Bit Score: 115.91 E-value: 4.30e-27
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 76 ISYKELDDHSRALAAWLQSRGLVKGDRVAIMMPNILQYPITFTAVLRAGFVVVNVNPLYTPRELEHqlndagAKalvvLE 155
Cdd:PRK12582 81 VTYGEAKRAVDALAQALLDLGLDPGRPVMILSGNSIEHALMTLAAMQAGVPAAPVSPAYSLMSHDH------AK----LK 150
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 156 NFATTVEkalpsinvPNILVASMGDMLGFKGHIVNLV-VRRVKKMVPAWNIPGhVRFKDALA----QGRAKSFNPVpvqG 230
Cdd:PRK12582 151 HLFDLVK--------PRVVFAQSGAPFARALAALDLLdVTVVHVTGPGEGIAS-IAFADLAAtpptAAVAAAIAAI---T 218
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 231 CD-IAFLQYTGGTTGISKGAMLTHSNILANVEQMNLWMDVAfRNKGKPKALNFvcaLPLYHIFALTVNAMIGMKLGArNI 309
Cdd:PRK12582 219 PDtVAKYLFTSGSTGMPKAVINTQRMMCANIAMQEQLRPRE-PDPPPPVSLDW---MPWNHTMGGNANFNGLLWGGG-TL 293
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 310 LIPNPRDIPSFVKELKKYPVHIFP--------GLNTLFNGLMNNPDFQTLDFKPLILTLGGGMAVQRPVAERWQHM---- 377
Cdd:PRK12582 294 YIDDGKPLPGMFEETIRNLREISPtvygnvpaGYAMLAEAMEKDDALRRSFFKNLRLMAYGGATLSDDLYERMQALavrt 373
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 378 TGCHI--TEGYGLSETSPVACANALDaTEFTGTIGLPMPstdvvirdddGSDLLLGKVG---EICVRGPQVMKGYWNRPE 452
Cdd:PRK12582 374 TGHRIpfYTGYGATETAPTTTGTHWD-TERVGLIGLPLP----------GVELKLAPVGdkyEVRVKGPNVTPGYHKDPE 442
|
410 420
....*....|....*....|....
gi 479816261 453 ETARAIMADGFFRTGDMG-FMDER 475
Cdd:PRK12582 443 LTAAAFDEEGFYRLGDAArFVDPD 466
|
|
| PRK12316 |
PRK12316 |
peptide synthase; Provisional |
66-579 |
6.78e-27 |
|
peptide synthase; Provisional
Pssm-ID: 237054 [Multi-domain] Cd Length: 5163 Bit Score: 116.60 E-value: 6.78e-27
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 66 SPAFTCMGKDISYKELDDHSRALAAWLQSRGLVKGDRVAIMMPNILQYPITFTAVLRAGFVVVNVNPLYTPRELEHQLND 145
Cdd:PRK12316 3073 AVALAFGEQRLSYAELNRRANRLAHRLIERGVGPDVLVGVAVERSLEMVVGLLAILKAGGAYVPLDPEYPEERLAYMLED 3152
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 146 AGAKALVVLENFAttvekaLPSIN-VPNILVasmgdmlgfkghivnlvvrrvkkmvpawnipghvrfkDALAQGRAKSFN 224
Cdd:PRK12316 3153 SGAQLLLSQSHLR------LPLAQgVQVLDL-------------------------------------DRGDENYAEANP 3189
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 225 PVPVQGCDIAFLQYTGGTTGISKGAMLTHSNILANVEQMNLWMDVAFRNKGKPKALNFVCALPLYHIFALTVNAMIgmKL 304
Cdd:PRK12316 3190 AIRTMPENLAYVIYTSGSTGKPKGVGIRHSALSNHLCWMQQAYGLGVGDRVLQFTTFSFDVFVEELFWPLMSGARV--VL 3267
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 305 GARNIlipnPRDIPSFVKELKKYPVHIFPGLNTLFNGLMNNPDFQtlDFKPLILTLGGGMAVQRPVAERWqhMTGCHITE 384
Cdd:PRK12316 3268 AGPED----WRDPALLVELINSEGVDVLHAYPSMLQAFLEEEDAH--RCTSLKRIVCGGEALPADLQQQV--FAGLPLYN 3339
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 385 GYGLSETSPVACANAL-DATEFTGTIGLPMPSTDVVIRDDDGSDLLLGKVGEICVRGPQVMKGYWNRPEETARAIMADGF 463
Cdd:PRK12316 3340 LYGPTEATITVTHWQCvEEGKDAVPIGRPIANRACYILDGSLEPVPVGALGELYLGGEGLARGYHNRPGLTAERFVPDPF 3419
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 464 ------FRTGDMGFMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVAAEHPGVLESAAVGIpnehSGEVVKLYVVRRDPE 537
Cdd:PRK12316 3420 vpgerlYRTGDLARYRADGVIEYIGRVDHQVKIRGFRIELGEIEARLLEHPWVREAVVLAV----DGRQLVAYVVPEDEA 3495
|
490 500 510 520
....*....|....*....|....*....|....*....|...
gi 479816261 538 LT-EDEVKAFCAERLTNYKRPREVEFRESLPKSNVGKILRREL 579
Cdd:PRK12316 3496 GDlREALKAHLKASLPEYMVPAHLLFLERMPLTPNGKLDRKAL 3538
|
|
| PLN02430 |
PLN02430 |
long-chain-fatty-acid-CoA ligase |
211-507 |
3.18e-26 |
|
long-chain-fatty-acid-CoA ligase
Pssm-ID: 178049 [Multi-domain] Cd Length: 660 Bit Score: 113.37 E-value: 3.18e-26
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 211 FKDALAQGRAKSFNPVPVQGCDIAFLQYTGGTTGISKGAMLTHSNILANVEQMNLWMDvAFRNKGKPKALnFVCALPLYH 290
Cdd:PLN02430 200 WIDFLHMGKENPSETNPPKPLDICTIMYTSGTSGDPKGVVLTHEAVATFVRGVDLFME-QFEDKMTHDDV-YLSFLPLAH 277
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 291 I-------FALTVNAMIGMKLGARNILIPN------------PRDIPSFVKELKKYPVHIFPGLNTLFNGL-------MN 344
Cdd:PLN02430 278 IldrmieeYFFRKGASVGYYHGDLNALRDDlmelkptllagvPRVFERIHEGIQKALQELNPRRRLIFNALykyklawMN 357
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 345 --------NPDFQTLDFKPLILTLGG-------GMAVQRPVAERWQHMTGC-HITEGYGLSETSPVACANALDATEFTGT 408
Cdd:PLN02430 358 rgyshkkaSPMADFLAFRKVKAKLGGrlrllisGGAPLSTEIEEFLRVTSCaFVVQGYGLTETLGPTTLGFPDEMCMLGT 437
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 409 IGLPMPSTDVVIRD--DDGSDLLlGK--VGEICVRGPQVMKGYWNRPEETaRAIMADGFFRTGDMGFMDERGYTKIVDRK 484
Cdd:PLN02430 438 VGAPAVYNELRLEEvpEMGYDPL-GEppRGEICVRGKCLFSGYYKNPELT-EEVMKDGWFHTGDIGEILPNGVLKIIDRK 515
|
330 340
....*....|....*....|...
gi 479816261 485 KDMILVSgfnvypnEIEEVAAEH 507
Cdd:PLN02430 516 KNLIKLS-------QGEYVALEY 531
|
|
| PRK00174 |
PRK00174 |
acetyl-CoA synthetase; Provisional |
74-581 |
8.29e-25 |
|
acetyl-CoA synthetase; Provisional
Pssm-ID: 234677 [Multi-domain] Cd Length: 637 Bit Score: 109.07 E-value: 8.29e-25
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 74 KDISYKELDDHSRALAAWLQSRGLVKGDRVAIMMPNILQYPITFTAVLR---------AGFvvvnvnplyTPRELEHQLN 144
Cdd:PRK00174 97 RKITYRELHREVCRFANALKSLGVKKGDRVAIYMPMIPEAAVAMLACARigavhsvvfGGF---------SAEALADRII 167
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 145 DAGAKALVVLE---------NFATTVEKAL---PSI-NVpnilvasmgdmlgfkghivnLVVRRVKKMVPaWNiPGHVRF 211
Cdd:PRK00174 168 DAGAKLVITADegvrggkpiPLKANVDEALancPSVeKV--------------------IVVRRTGGDVD-WV-EGRDLW 225
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 212 KDALAQGRAKSFNPVPVQGCDIAFLQYTGGTTGISKG-----------AMLTHSNILanveqmnlwmDvafrnkgkpkal 280
Cdd:PRK00174 226 WHELVAGASDECEPEPMDAEDPLFILYTSGSTGKPKGvlhttggylvyAAMTMKYVF----------D------------ 283
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 281 nfvcalplYH---IFALTvnAMIG------------MKLGARNIL---IPNPRDIPSFVKELKKYPVHIF---PglnTLF 339
Cdd:PRK00174 284 --------YKdgdVYWCT--ADVGwvtghsyivygpLANGATTLMfegVPNYPDPGRFWEVIDKHKVTIFytaP---TAI 350
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 340 NGLMNNPD--FQTLDFKPLILtLGggmAVQRPV-------------AER-------WQHMTGCH-ITEGYGLSETSPvac 396
Cdd:PRK00174 351 RALMKEGDehPKKYDLSSLRL-LG---SVGEPInpeawewyykvvgGERcpivdtwWQTETGGImITPLPGATPLKP--- 423
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 397 analdateftGTIGLPMPSTDVVIRDDDGSDLLLGKVGEICVRG--PQVMKGYWNRPE---ETARAIMADGFFrTGDMGF 471
Cdd:PRK00174 424 ----------GSATRPLPGIQPAVVDEEGNPLEGGEGGNLVIKDpwPGMMRTIYGDHErfvKTYFSTFKGMYF-TGDGAR 492
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 472 MDERGYTKIVDRKKDMILVSGFNVYPNEIEEVAAEHPGVLESAAVGIPNEHSGEVVKLYVVRRDPELTED----EVKAFC 547
Cdd:PRK00174 493 RDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVAHPKVAEAAVVGRPDDIKGQGIYAFVTLKGGEEPSDelrkELRNWV 572
|
570 580 590
....*....|....*....|....*....|....
gi 479816261 548 AERLTNYKRPREVEFRESLPKSNVGKILRRELRD 581
Cdd:PRK00174 573 RKEIGPIAKPDVIQFAPGLPKTRSGKIMRRILRK 606
|
|
| ACSBG_like |
cd05933 |
Bubblegum-like very long-chain fatty acid CoA synthetase (VL-FACS); This family of very ... |
76-502 |
8.56e-25 |
|
Bubblegum-like very long-chain fatty acid CoA synthetase (VL-FACS); This family of very long-chain fatty acid CoA synthetase is named bubblegum because Drosophila melanogaster mutant bubblegum (BGM) has elevated levels of very-long-chain fatty acids (VLCFA) caused by a defective gene of this family. The human homolog (hsBG) has been characterized as a very long chain fatty acid CoA synthetase that functions specifically in the brain; hsBG may play a central role in brain VLCFA metabolism and myelinogenesis. VL-FACS is involved in the first reaction step of very long chain fatty acid degradation. It catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.
Pssm-ID: 341256 [Multi-domain] Cd Length: 596 Bit Score: 108.60 E-value: 8.56e-25
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 76 ISYKELDDHSRALAAWLQSRGLVKGDRVAIMMPNILQYPITFTAVLRAGFVVVNVNPLYTPRELEHQLNDAGAKALVVlE 155
Cdd:cd05933 9 LTYKEYYEACRQAAKAFLKLGLERFHGVGILGFNSPEWFIAAVGAIFAGGIAVGIYTTNSPEACQYVAETSEANILVV-E 87
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 156 NfattvEKALPSINVPNILVASMGDMLGFKGHIVNlvvrrvKKmvpawniPGHVRFKDALAQGRAKSFNPV--------P 227
Cdd:cd05933 88 N-----QKQLQKILQIQDKLPHLKAIIQYKEPLKE------KE-------PNLYSWDEFMELGRSIPDEQLdaiissqkP 149
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 228 VQGCdiaFLQYTGGTTGISKGAMLTHSNILANVEQMNLWMDVAFRNKGKPkalNFVCALPLYHIFALTVNAMIGMKLGAR 307
Cdd:cd05933 150 NQCC---TLIYTSGTTGMPKGVMLSHDNITWTAKAASQHMDLRPATVGQE---SVVSYLPLSHIAAQILDIWLPIKVGGQ 223
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 308 nILIPNPRDIP-SFVKELKKYPVHIFPGLNTLFNGL---MNNPDFQTLDFKPLILT---------------------LGG 362
Cdd:cd05933 224 -VYFAQPDALKgTLVKTLREVRPTAFMGVPRVWEKIqekMKAVGAKSGTLKRKIASwakgvgletnlklmggespspLFY 302
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 363 GMAVQRpVAERWQHMTG---CH-----------------------ITEGYGLSETSPVACANALDATEFTgTIGLPMPST 416
Cdd:cd05933 303 RLAKKL-VFKKVRKALGldrCQkfftgaapisretlefflslnipIMELYGMSETSGPHTISNPQAYRLL-SCGKALPGC 380
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 417 DVVI--RDDDGSdlllgkvGEICVRGPQVMKGYWNRPEETARAIMADGFFRTGDMGFMDERGYTKIVDRKKDMILVS-GF 493
Cdd:cd05933 381 KTKIhnPDADGI-------GEICFWGRHVFMGYLNMEDKTEEAIDEDGWLHSGDLGKLDEDGFLYITGRIKELIITAgGE 453
|
....*....
gi 479816261 494 NVYPNEIEE 502
Cdd:cd05933 454 NVPPVPIED 462
|
|
| PTZ00216 |
PTZ00216 |
acyl-CoA synthetase; Provisional |
74-485 |
1.41e-24 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 240316 [Multi-domain] Cd Length: 700 Bit Score: 108.53 E-value: 1.41e-24
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 74 KDISYKELDDHSRALAAWLQSRGLVKGDRVAIMMPNILQYPITFTAVLRAGFVVVNVNPLYTPRELEHQLNDAGAKALVV 153
Cdd:PTZ00216 120 RYITYAELWERIVNFGRGLAELGLTKGSNVAIYEETRWEWLASIYGIWSQSMVAATVYANLGEDALAYALRETECKAIVC 199
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 154 lenfattvekalPSINVPNILvasmgDMLGfKGHIVNLVVRRVKKMVPAWNIPGH--VRFKDALAQGR-AKSFNPVPVQG 230
Cdd:PTZ00216 200 ------------NGKNVPNLL-----RLMK-SGGMPNTTIIYLDSLPASVDTEGCrlVAWTDVVAKGHsAGSHHPLNIPE 261
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 231 -CDI-AFLQYTGGTTGISKGAMLTHSNILANVEQMNLWM-DVAfrnkGKPKALNFVCA-LPLYHIFALTV-NAMIgmklg 305
Cdd:PTZ00216 262 nNDDlALIMYTSGTTGDPKGVMHTHGSLTAGILALEDRLnDLI----GPPEEDETYCSyLPLAHIMEFGVtNIFL----- 332
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 306 ARNILI--PNPRDIPS-FVK---ELKKY-PV------HIF-------------PG--------------LNTLFNGlMNN 345
Cdd:PTZ00216 333 ARGALIgfGSPRTLTDtFARphgDLTEFrPVfligvpRIFdtikkaveaklppVGslkrrvfdhayqsrLRALKEG-KDT 411
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 346 PDFQTLDFKPLILTLGGGMAVQ----RPVAERWQHMT----GChITEGYGLSETspVACAnaldATEFTGTI-----GLP 412
Cdd:PTZ00216 412 PYWNEKVFSAPRAVLGGRVRAMlsggGPLSAATQEFVnvvfGM-VIQGWGLTET--VCCG----GIQRTGDLepnavGQL 484
|
410 420 430 440 450 460 470
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 479816261 413 MPSTDVVIRDDDG---SDLLLGKvGEICVRGPQVMKGYWNRPEETARAIMADGFFRTGDMGFMDERGYTKIVDRKK 485
Cdd:PTZ00216 485 LKGVEMKLLDTEEykhTDTPEPR-GEILLRGPFLFKGYYKQEELTREVLDEDGWFHTGDVGSIAANGTLRIIGRVK 559
|
|
| PLN02614 |
PLN02614 |
long-chain acyl-CoA synthetase |
13-525 |
5.64e-24 |
|
long-chain acyl-CoA synthetase
Pssm-ID: 166255 [Multi-domain] Cd Length: 666 Bit Score: 106.64 E-value: 5.64e-24
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 13 VDSAKVAPTGeaRPAVEKIWLKSYPA-GVPHDINlsAATSISDMISASCRQFANSPAFT-------CMGKDI--SYKELD 82
Cdd:PLN02614 11 VEEGKEGSDG--RPSVGPVYRSIFAKdGFPNPIE--GMDSCWDVFRMSVEKYPNNPMLGrreivdgKPGKYVwqTYQEVY 86
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 83 DHSRALAAWLQSRGLVKGDRVAIMMPNILQYPITFTAVLRAGFVVVnvnPLYTPRelehqlnDAGAKALVVLENFATTV- 161
Cdd:PLN02614 87 DIVIKLGNSLRSVGVKDEAKCGIYGANSPEWIISMEACNAHGLYCV---PLYDTL-------GAGAVEFIISHSEVSIVf 156
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 162 --EKALPSI--NVPNIlVASMGDMLGFKGhivnlVVRRVKKMVPAWNIPGHVrFKDALAQGRAKSFNPVPVQGCDIAFLQ 237
Cdd:PLN02614 157 veEKKISELfkTCPNS-TEYMKTVVSFGG-----VSREQKEEAETFGLVIYA-WDEFLKLGEGKQYDLPIKKKSDICTIM 229
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 238 YTGGTTGISKGAMLTHSNILANVEQMNLWMDVAfrNKGKPKALNFVCALPLYHIF-------ALTVNAMIGMKLGARNIL 310
Cdd:PLN02614 230 YTSGTTGDPKGVMISNESIVTLIAGVIRLLKSA--NAALTVKDVYLSYLPLAHIFdrvieecFIQHGAAIGFWRGDVKLL 307
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 311 IPNPRDI-PSFVKELKKYPVHIFPGLNTLFNG--------------------------LMNNPDFQTLDFKPLILTLGGG 363
Cdd:PLN02614 308 IEDLGELkPTIFCAVPRVLDRVYSGLQKKLSDggflkkfvfdsafsykfgnmkkgqshVEASPLCDKLVFNKVKQGLGGN 387
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 364 MAV----QRPVAERWQH----MTGCHITEGYGLSETSPVACANALDATEFTGTIGLPMPSTDVVIRD--DDGSDLLLGKV 433
Cdd:PLN02614 388 VRIilsgAAPLASHVESflrvVACCHVLQGYGLTESCAGTFVSLPDELDMLGTVGPPVPNVDIRLESvpEMEYDALASTP 467
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 434 -GEICVRGPQVMKGYWNRpEETARAIMADGFFRTGDMGFMDERGYTKIVDRKKDMI-LVSGFNVYPNEIEEVAAEHPGV- 510
Cdd:PLN02614 468 rGEICIRGKTLFSGYYKR-EDLTKEVLIDGWLHTGDVGEWQPNGSMKIIDRKKNIFkLSQGEYVAVENIENIYGEVQAVd 546
|
570 580
....*....|....*....|....*
gi 479816261 511 --------LESAAVGI--PNEHSGE 525
Cdd:PLN02614 547 svwvygnsFESFLVAIanPNQQILE 571
|
|
| FCS |
cd05921 |
Feruloyl-CoA synthetase (FCS); Feruloyl-CoA synthetase is an essential enzyme in the feruloyl ... |
76-556 |
2.29e-23 |
|
Feruloyl-CoA synthetase (FCS); Feruloyl-CoA synthetase is an essential enzyme in the feruloyl acid degradation pathway and enables some proteobacteria to grow on media containing feruloyl acid as the sole carbon source. It catalyzes the transfer of CoA to the carboxyl group of ferulic acid, which then forms feruloyl-CoA in the presence of ATP and Mg2. The resulting feruloyl-CoA is further degraded to vanillin and acetyl-CoA. Feruloyl-CoA synthetase (FCS) is a subfamily of the adenylate-forming enzymes superfamily.
Pssm-ID: 341245 [Multi-domain] Cd Length: 561 Bit Score: 104.05 E-value: 2.29e-23
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 76 ISYKELDDHSRALAAWLQSRGLVKGDRVAIMMPNILQYPITFTAVLRAGFVVVNVNPLYTPRELEH-QLNDAGAK---AL 151
Cdd:cd05921 26 VTYAEALRQVRAIAQGLLDLGLSAERPLLILSGNSIEHALMALAAMYAGVPAAPVSPAYSLMSQDLaKLKHLFELlkpGL 105
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 152 VVLENfATTVEKALPSINVPNI-LVASMGDMLGfkghivnlvvrrvkkmvpawniPGHVRFKDALAQ-GRAKSFNPVPVQ 229
Cdd:cd05921 106 VFAQD-AAPFARALAAIFPLGTpLVVSRNAVAG----------------------RGAISFAELAATpPTAAVDAAFAAV 162
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 230 GCD-IAFLQYTGGTTGISKGAMLTHSNILANVEQMnlwMDVAFRNKGKPKALnfVCALPLYHIFAltVNAMIGMKL-GAR 307
Cdd:cd05921 163 GPDtVAKFLFTSGSTGLPKAVINTQRMLCANQAML---EQTYPFFGEEPPVL--VDWLPWNHTFG--GNHNFNLVLyNGG 235
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 308 NILI----PNPRDIPSFVKELKKY-PVHIF---PGLNTLFNGLMNNPDFQTLDFKPLILTLGGGMAVQRPVAERWQHMTG 379
Cdd:cd05921 236 TLYIddgkPMPGGFEETLRNLREIsPTVYFnvpAGWEMLVAALEKDEALRRRFFKRLKLMFYAGAGLSQDVWDRLQALAV 315
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 380 CHITE------GYGLSETSPVACaNALDATEFTGTIGLPMPSTDVVIRDDDGsdlllgKVgEICVRGPQVMKGYWNRPEE 453
Cdd:cd05921 316 ATVGEripmmaGLGATETAPTAT-FTHWPTERSGLIGLPAPGTELKLVPSGG------KY-EVRVKGPNVTPGYWRQPEL 387
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 454 TARAIMADGFFRTGDMG-FMD----ERGYtkIVDRK--KDMILVSG--FNVYPNEIEEVAAEHPGVLESAAVGIPNEHSG 524
Cdd:cd05921 388 TAQAFDEEGFYCLGDAAkLADpddpAKGL--VFDGRvaEDFKLASGtwVSVGPLRARAVAACAPLVHDAVVAGEDRAEVG 465
|
490 500 510 520
....*....|....*....|....*....|....*....|...
gi 479816261 525 EVV--KLYVVRR--------DPE-LTEDEVKAFCAERLTNYKR 556
Cdd:cd05921 466 ALVfpDLLACRRlvglqeasDAEvLRHAKVRAAFRDRLAALNG 508
|
|
| hsFATP4_like |
cd05939 |
Fatty acid transport proteins (FATP), including FATP4 and FATP1, and similar proteins; Fatty ... |
77-580 |
3.15e-23 |
|
Fatty acid transport proteins (FATP), including FATP4 and FATP1, and similar proteins; Fatty acid transport protein (FATP) transports long-chain or very-long-chain fatty acids across the plasma membrane. At least five copies of FATPs are identified in mammalian cells. This family includes FATP4, FATP1, and homologous proteins. Each FATP has unique patterns of tissue distribution. FATP4 is mainly expressed in the brain, testis, colon and kidney. FATPs also have fatty acid CoA synthetase activity, thus playing dual roles as fatty acid transporters and its activation enzymes. FATPs are the key players in the trafficking of exogenous fatty acids into the cell and in intracellular fatty acid homeostasis.
Pssm-ID: 341262 [Multi-domain] Cd Length: 474 Bit Score: 102.89 E-value: 3.15e-23
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 77 SYKELDDHSRALAAWLQSRGLVKGDRVAIMMPNILQYPITFTAVLRAGFVVVNVNPLYTPRELEHQLNDAGAKALVV--L 154
Cdd:cd05939 5 TFRELNEYSNKVANFFQAQGYRSGDVVALFMENRLEFVALWLGLAKIGVETALINSNLRLESLLHCITVSKAKALIFnlL 84
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 155 ENFATTVEKALPSInvpnilvasmgDMLGFKghivnlvvrrvkkmvpawnipghvrfkdalaqgraksfnpvpvqgcDIA 234
Cdd:cd05939 85 DPLLTQSSTEPPSQ-----------DDVNFR----------------------------------------------DKL 107
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 235 FLQYTGGTTGISKGAMLTHSNILAnveqmnLWMDVAFRNKGKPKALNFVCaLPLYH------------IFALTVnaMIGM 302
Cdd:cd05939 108 FYIYTSGTTGLPKAAVIVHSRYYR------IAAGAYYAFGMRPEDVVYDC-LPLYHsaggimgvgqalLHGSTV--VIRK 178
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 303 KLGARNilipnprdipsFVKELKKYPVHIFPGLNTLFNGLMNNPDFQTLDFKPLILTLGGGMavqRPvaERWQHMTG--- 379
Cdd:cd05939 179 KFSASN-----------FWDDCVKYNCTIVQYIGEICRYLLAQPPSEEEQKHNVRLAVGNGL---RP--QIWEQFVRrfg 242
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 380 -CHITEGYGLSE--TSPVACANALDATEFTGTIGLPMPSTDVVIRDDDGSDLLLGKVGeICVR------GPQVMK----- 445
Cdd:cd05939 243 iPQIGEFYGATEgnSSLVNIDNHVGACGFNSRILPSVYPIRLIKVDEDTGELIRDSDG-LCIPcqpgepGLLVGKiiqnd 321
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 446 ------GYWNRpEETARAIMADGF------FRTGDMGFMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVAAEHPGVLES 513
Cdd:cd05939 322 plrrfdGYVNE-GATNKKIARDVFkkgdsaFLSGDVLVMDELGYLYFKDRTGDTFRWKGENVSTTEVEGILSNVLGLEDV 400
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 479816261 514 AAVGIPNEHS-GEVVKLYVVRRDPELTEDEVKAFCAERLTNYKRPREVEFRESLPKSNVGKILRRELR 580
Cdd:cd05939 401 VVYGVEVPGVeGRAGMAAIVDPERKVDLDRFSAVLAKSLPPYARPQFIRLLPEVDKTGTFKLQKTDLQ 468
|
|
| A_NRPS_ApnA-like |
cd17644 |
similar to adenylation domain of anabaenopeptin synthetase (ApnA); This family of the ... |
73-579 |
4.73e-23 |
|
similar to adenylation domain of anabaenopeptin synthetase (ApnA); This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes Planktothrix agardhii anabaenopeptin synthetase (ApnA A1), which is capable of activating two chemically distinct amino acids (Arg and Tyr). Structural studies show that the architecture of the active site forces Arg to adopt a Tyr-like conformation, thus explaining the bispecificity. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341299 [Multi-domain] Cd Length: 465 Bit Score: 102.51 E-value: 4.73e-23
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 73 GKDISYKELDDHSRALAAWLQSRGLVKGDRVAIMMPNILQYPITFTAVLRAGFVVVNVNPLYTPRELEHQLNDAGAKALV 152
Cdd:cd17644 23 DQQLTYEELNTKANQLAHYLQSLGVKSESLVGICVERSLEMIIGLLAILKAGGAYVPLDPNYPQERLTYILEDAQISVLL 102
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 153 VlenfattvekalpsinvpnilvasmgdmlgfkghivnlvvrrvkkmvpawnipghvrfkdalaqgraksfnpvpvQGCD 232
Cdd:cd17644 103 T---------------------------------------------------------------------------QPEN 107
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 233 IAFLQYTGGTTGISKGAMLTHSNILanveQMNLWMDVAFRNKGKPKALNFVCalplyhiFALTVNA-MIGMKL--GARNI 309
Cdd:cd17644 108 LAYVIYTSGSTGKPKGVMIEHQSLV----NLSHGLIKEYGITSSDRVLQFAS-------IAFDVAAeEIYVTLlsGATLV 176
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 310 LIPNP--RDIPSFVKELKKYPVHIFPGLNTLFNGLMNN--PDFQTLDFKP-LILTlgGGMAVQRPVAERWQHMTGCHIT- 383
Cdd:cd17644 177 LRPEEmrSSLEDFVQYIQQWQLTVLSLPPAYWHLLVLEllLSTIDLPSSLrLVIV--GGEAVQPELVRQWQKNVGNFIQl 254
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 384 -EGYGLSE-TSPVACANALDATEFTGT---IGLPMPSTDVVIRDDDGSDLLLGKVGEICVRGPQVMKGYWNRPEETARAI 458
Cdd:cd17644 255 iNVYGPTEaTIAATVCRLTQLTERNITsvpIGRPIANTQVYILDENLQPVPVGVPGELHIGGVGLARGYLNRPELTAEKF 334
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 459 MADGF--------FRTGDMGFMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVAAEHPGVLESAAVGIPNEHSGEVVKLY 530
Cdd:cd17644 335 ISHPFnsseserlYKTGDLARYLPDGNIEYLGRIDNQVKIRGFRIELGEIEAVLSQHNDVKTAVVIVREDQPGNKRLVAY 414
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|
gi 479816261 531 VVRRDPE-LTEDEVKAFCAERLTNYKRPREVEFRESLPKSNVGKILRREL 579
Cdd:cd17644 415 IVPHYEEsPSTVELRQFLKAKLPDYMIPSAFVVLEELPLTPNGKIDRRAL 464
|
|
| PRK08180 |
PRK08180 |
feruloyl-CoA synthase; Reviewed |
76-475 |
8.96e-23 |
|
feruloyl-CoA synthase; Reviewed
Pssm-ID: 236175 [Multi-domain] Cd Length: 614 Bit Score: 102.65 E-value: 8.96e-23
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 76 ISYKELDDHSRALAAWLQSRGLVKGDRVAIMMPNILQYPITFTAVLRAGFVVVNVNPLYT-----PRELEHQLnDAGAKA 150
Cdd:PRK08180 70 LTYAEALERVRAIAQALLDRGLSAERPLMILSGNSIEHALLALAAMYAGVPYAPVSPAYSlvsqdFGKLRHVL-ELLTPG 148
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 151 LVVLENfATTVEKALPSINVPNILVasmgdmlgfkghivnlVVRRvkkmvPAWNIPGHVRFKDALAQGRAKSFNPV--PV 228
Cdd:PRK08180 149 LVFADD-GAAFARALAAVVPADVEV----------------VAVR-----GAVPGRAATPFAALLATPPTAAVDAAhaAV 206
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 229 QGCDIAFLQYTGGTTGISKGAMLTHSNILANVEQMNLWMdvAFRNKGKPKalnFVCALPLYHIFAltVNAMIGMKLgaRN 308
Cdd:PRK08180 207 GPDTIAKFLFTSGSTGLPKAVINTHRMLCANQQMLAQTF--PFLAEEPPV---LVDWLPWNHTFG--GNHNLGIVL--YN 277
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 309 ---ILI----PNPRDIPSFVKELKKYPVHIF---P-GLNTLFNGLMNNPDFQTLDFKPLILTLGGGMAVQRPVAERWQHM 377
Cdd:PRK08180 278 ggtLYIddgkPTPGGFDETLRNLREISPTVYfnvPkGWEMLVPALERDAALRRRFFSRLKLLFYAGAALSQDVWDRLDRV 357
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 378 TGCHITE------GYGLSETSPvACANALDATEFTGTIGLPMPSTDVVIRDDDGsdlllgKVgEICVRGPQVMKGYWNRP 451
Cdd:PRK08180 358 AEATCGErirmmtGLGMTETAP-SATFTTGPLSRAGNIGLPAPGCEVKLVPVGG------KL-EVRVKGPNVTPGYWRAP 429
|
410 420
....*....|....*....|....*
gi 479816261 452 EETARAIMADGFFRTGDMG-FMDER 475
Cdd:PRK08180 430 ELTAEAFDEEGYYRSGDAVrFVDPA 454
|
|
| PRK05691 |
PRK05691 |
peptide synthase; Validated |
52-579 |
1.74e-22 |
|
peptide synthase; Validated
Pssm-ID: 235564 [Multi-domain] Cd Length: 4334 Bit Score: 102.94 E-value: 1.74e-22
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 52 ISDMISASCRQFANSPAFTCMGKDISYKELDDHSRALAAWLQSRGLVKGDRVAIMMPNILQYPITFTAVLRAGFVVVNVN 131
Cdd:PRK05691 2190 LHGLFAAQAARTPQAPALTFAGQTLSYAELDARANRLARALRERGVGPQVRVGLALERSLEMVVGLLAILKAGGAYVPLD 2269
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 132 PLYTPRELEHQLNDAGAKALvvLENFAttvekalpsinvpniLVASMGDMlgfkghivnlvvrrvKKMVPAWNIpghvrf 211
Cdd:PRK05691 2270 PEYPLERLHYMIEDSGIGLL--LSDRA---------------LFEALGEL---------------PAGVARWCL------ 2311
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 212 KDALAQGRAKSFNPVP--VQGCDIAFLQYTGGTTGISKGAMLTHSNIL----ANVEQMNLWMDVafrnkgkpkalnfvCA 285
Cdd:PRK05691 2312 EDDAAALAAYSDAPLPflSLPQHQAYLIYTSGSTGKPKGVVVSHGEIAmhcqAVIERFGMRADD--------------CE 2377
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 286 LPLYHI-F-ALTVNAMIGMKLGARNILIP----NPRDIPSFVKELKKYPVHIFPGLNTLFNGLMNNPDfQTLdfkPLILT 359
Cdd:PRK05691 2378 LHFYSInFdAASERLLVPLLCGARVVLRAqgqwGAEEICQLIREQQVSILGFTPSYGSQLAQWLAGQG-EQL---PVRMC 2453
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 360 LGGGMAVqrpVAERWQHMTGCHITE----GYGLSET--SPVAC--ANALDATEFTGTIGLPMPSTDVVIRDDDGSDLLLG 431
Cdd:PRK05691 2454 ITGGEAL---TGEHLQRIRQAFAPQlffnAYGPTETvvMPLAClaPEQLEEGAASVPIGRVVGARVAYILDADLALVPQG 2530
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 432 KVGEICVRGPQVMKGYWNRPEETARAIMADGF-------FRTGDMGFMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVA 504
Cdd:PRK05691 2531 ATGELYVGGAGLAQGYHDRPGLTAERFVADPFaadggrlYRTGDLVRLRADGLVEYVGRIDHQVKIRGFRIELGEIESRL 2610
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 505 AEHPGVLESAAVGIPNEhSGEVVKLYVVRRDPELTEDE-------VKAFCAERLTNYKRPREVEFRESLPKSNVGKILRR 577
Cdd:PRK05691 2611 LEHPAVREAVVLALDTP-SGKQLAGYLVSAVAGQDDEAqaalreaLKAHLKQQLPDYMVPAHLILLDSLPLTANGKLDRR 2689
|
..
gi 479816261 578 EL 579
Cdd:PRK05691 2690 AL 2691
|
|
| hsFATP2a_ACSVL_like |
cd05938 |
Fatty acid transport proteins (FATP) including hsFATP2, hsFATP5, and hsFATP6, and similar ... |
73-566 |
2.98e-22 |
|
Fatty acid transport proteins (FATP) including hsFATP2, hsFATP5, and hsFATP6, and similar proteins; Fatty acid transport proteins (FATP) of this family transport long-chain or very-long-chain fatty acids across the plasma membrane. At least five copies of FATPs are identified in mammalian cells. This family includes hsFATP2, hsFATP5, and hsFATP6, and similar proteins. Each FATP has unique patterns of tissue distribution. These FATPs also have fatty acid CoA synthetase activity, thus playing dual roles as fatty acid transporters and its activation enzymes. The hsFATP proteins exist in two splice variants; the b variant, lacking exon 3, has no acyl-CoA synthetase activity. FATPs are key players in the trafficking of exogenous fatty acids into the cell and in intracellular fatty acid homeostasis.
Pssm-ID: 341261 [Multi-domain] Cd Length: 537 Bit Score: 100.44 E-value: 2.98e-22
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 73 GKDISYKELDDHSRALA-AWLQSRGLVKGDRVAIMMPNILQYPITFTAVLRAGFVVVNVNPLYTPRELEHQLNDAGAKAL 151
Cdd:cd05938 3 GETYTYRDVDRRSNQAArALLAHAGLRPGDTVALLLGNEPAFLWIWLGLAKLGCPVAFLNTNIRSKSLLHCFRCCGAKVL 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 152 VVLENFATTVEKALPSINVPNILVASMGdmlgfkghivnlvvrrvkkmvPAWNIPGHVRFKDALAqgrAKSFNPVP---- 227
Cdd:cd05938 83 VVAPELQEAVEEVLPALRADGVSVWYLS---------------------HTSNTEGVISLLDKVD---AASDEPVPaslr 138
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 228 --VQGCDIAFLQYTGGTTGISKGAMLTHsnilanveqMNLWMDVAFRNKGKPKALNFV-CALPLYHIFALTVNAMIGMKL 304
Cdd:cd05938 139 ahVTIKSPALYIYTSGTTGLPKAARISH---------LRVLQCSGFLSLCGVTADDVIyITLPLYHSSGFLLGIGGCIEL 209
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 305 GARNILIPNpRDIPSFVKELKKYPVHIFPGLNTLFNGLMNNPdfQTLDFK--PLILTLGGGMavqRP-VAERWQHMTG-C 380
Cdd:cd05938 210 GATCVLKPK-FSASQFWDDCRKHNVTVIQYIGELLRYLCNQP--QSPNDRdhKVRLAIGNGL---RAdVWREFLRRFGpI 283
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 381 HITEGYGLSETSpVACANaldateFTGTIG------------LPM------PSTDVVIRDDDG---------SDLLLGKv 433
Cdd:cd05938 284 RIREFYGSTEGN-IGFFN------YTGKIGavgrvsylykllFPFelikfdVEKEEPVRDAQGfcipvakgePGLLVAK- 355
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 434 geicVRGPQVMKGYWNRPEETARAIMADGF------FRTGDMGFMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVAAEH 507
Cdd:cd05938 356 ----ITQQSPFLGYAGDKEQTEKKLLRDVFkkgdvyFNTGDLLVQDQQNFLYFHDRVGDTFRWKGENVATTEVADVLGLL 431
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|.
gi 479816261 508 PGVLESAAVGIPNE-HSGEVVKLYVVRRDP-ELTEDEVKAFCAERLTNYKRPREVEFRESL 566
Cdd:cd05938 432 DFLQEVNVYGVTVPgHEGRIGMAAVKLKPGhEFDGKKLYQHVREYLPAYARPRFLRIQDSL 492
|
|
| entF |
PRK10252 |
enterobactin non-ribosomal peptide synthetase EntF; |
48-579 |
3.10e-22 |
|
enterobactin non-ribosomal peptide synthetase EntF;
Pssm-ID: 236668 [Multi-domain] Cd Length: 1296 Bit Score: 101.66 E-value: 3.10e-22
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 48 AATSISDMISASCRQFANSPAFTCMGKDISYKELDDHSRALAAWLQSRGLVKGDRVAIMMPNILQYPITFTAVLRAGFVV 127
Cdd:PRK10252 456 PETTLSALVAQQAAKTPDAPALADARYQFSYREMREQVVALANLLRERGVKPGDSVAVALPRSVFLTLALHAIVEAGAAW 535
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 128 VNVNPLYTPRELEHQLNDAGAKALVvlenfaTTVEKALPSINVPNILVASmgdmlgfkghivnlvvrrvkkmVPAWnipg 207
Cdd:PRK10252 536 LPLDTGYPDDRLKMMLEDARPSLLI------TTADQLPRFADVPDLTSLC----------------------YNAP---- 583
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 208 hvrfkDALAQGRAKsfnpVPVQGCDIAFLQYTGGTTGISKGAMLTHSNIlanVEQMnLWMDVAFRNKGKPKALNFVCAlp 287
Cdd:PRK10252 584 -----LAPQGAAPL----QLSQPHHTAYIIFTSGSTGRPKGVMVGQTAI---VNRL-LWMQNHYPLTADDVVLQKTPC-- 648
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 288 lyhIFALTV-NAMIGMKLGARNILIP-----NPRDIPSFVKELKKYPVHIFPGLNTLFnglMNNPDFQTLDFKPLILT-- 359
Cdd:PRK10252 649 ---SFDVSVwEFFWPFIAGAKLVMAEpeahrDPLAMQQFFAEYGVTTTHFVPSMLAAF---VASLTPEGARQSCASLRqv 722
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 360 LGGGMAVQRPVAERWQHMTGCHITEGYGLSETS------PvACANALDATefTGT---IGLPMPSTDVVIRDDDGSDLLL 430
Cdd:PRK10252 723 FCSGEALPADLCREWQQLTGAPLHNLYGPTEAAvdvswyP-AFGEELAAV--RGSsvpIGYPVWNTGLRILDARMRPVPP 799
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 431 GKVGEICVRGPQVMKGYWNRPEETARAIMADGF------FRTGDMGFMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVA 504
Cdd:PRK10252 800 GVAGDLYLTGIQLAQGYLGRPDLTASRFIADPFapgermYRTGDVARWLDDGAVEYLGRSDDQLKIRGQRIELGEIDRAM 879
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 505 AEHPGVLESAAVGI----PNEHSGEVVKL--YVVRRDPE-LTEDEVKAFCAERLTNYKRPREVEFRESLPKSNVGKILRR 577
Cdd:PRK10252 880 QALPDVEQAVTHACvinqAAATGGDARQLvgYLVSQSGLpLDTSALQAQLRERLPPHMVPVVLLQLDQLPLSANGKLDRK 959
|
..
gi 479816261 578 EL 579
Cdd:PRK10252 960 AL 961
|
|
| PRK07445 |
PRK07445 |
O-succinylbenzoic acid--CoA ligase; Reviewed |
239-581 |
1.03e-21 |
|
O-succinylbenzoic acid--CoA ligase; Reviewed
Pssm-ID: 236019 [Multi-domain] Cd Length: 452 Bit Score: 98.14 E-value: 1.03e-21
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 239 TGGTTGISKGAMLTHSNILANVEqmnlwmdvAFRNKGKPKALNFVCALPLYHIFALTVnAMIGMKLGARNILIPnprdip 318
Cdd:PRK07445 128 TGGSSGQIRFAIHTWETLTASVQ--------GFQRYFQLQQVNSFCVLPLYHVSGLMQ-FMRSFLTGGKLVILP------ 192
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 319 sfVKELKKYPVhifpgLNTlfnglmNNPDF---------QTL---------DFKpLILtLGGGMAVQRPVAERWQHmtGC 380
Cdd:PRK07445 193 --YKRLKSGQE-----LPP------NPSDFflslvptqlQRLlqlrpqwlaQFR-TIL-LGGAPAWPSLLEQARQL--QL 255
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 381 HITEGYGLSET-SPVAcanALDATEF-TGTIGL--PMPSTDVVIRDddgsdlllGKVGEICVRGPQVMKGYWnrPEetar 456
Cdd:PRK07445 256 RLAPTYGMTETaSQIA---TLKPDDFlAGNNSSgqVLPHAQITIPA--------NQTGNITIQAQSLALGYY--PQ---- 318
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 457 AIMADGFFRTGDMGFMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVAAEHPGVLESAAVGIPNEHSGEVVKLYVVRRDP 536
Cdd:PRK07445 319 ILDSQGIFETDDLGYLDAQGYLHILGRNSQKIITGGENVYPAEVEAAILATGLVQDVCVLGLPDPHWGEVVTAIYVPKDP 398
|
330 340 350 360
....*....|....*....|....*....|....*....|....*
gi 479816261 537 ELTEDEVKAFCAERLTNYKRPREVEFRESLPKSNVGKILRRELRD 581
Cdd:PRK07445 399 SISLEELKTAIKDQLSPFKQPKHWIPVPQLPRNPQGKINRQQLQQ 443
|
|
| prpE |
PRK10524 |
propionyl-CoA synthetase; Provisional |
74-580 |
1.03e-21 |
|
propionyl-CoA synthetase; Provisional
Pssm-ID: 182517 [Multi-domain] Cd Length: 629 Bit Score: 99.25 E-value: 1.03e-21
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 74 KDISYKELDDHSRALAAWLQSRGLVKGDRVAIMMPNILQYPITFTAVLRAGFVVVNVNPLYTPRELEHQLNDAGAKaLVV 153
Cdd:PRK10524 83 RTYTFRQLHDEVNRMAAMLRSLGVQRGDRVLIYMPMIAEAAFAMLACARIGAIHSVVFGGFASHSLAARIDDAKPV-LIV 161
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 154 LENFATTVEKALPSINvpniLVASMGDMLGFKGHIVNLVVRRVKKMvpAWNIPGHVRFkdalAQGRAKSFNP-VPVQ--- 229
Cdd:PRK10524 162 SADAGSRGGKVVPYKP----LLDEAIALAQHKPRHVLLVDRGLAPM--ARVAGRDVDY----ATLRAQHLGArVPVEwle 231
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 230 GCDIAFLQYTGGTTGISKGAM---------LTHSnilanveqmnlwMDVAFrnKGKPKALNFvCALPL-------YHIFA 293
Cdd:PRK10524 232 SNEPSYILYTSGTTGKPKGVQrdtggyavaLATS------------MDTIF--GGKAGETFF-CASDIgwvvghsYIVYA 296
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 294 LTVNAM---------------IGMKLGAR---NILIpnprDIPSFVKELKKYPV-----HIFPGLNTLFngLMNNPdfqt 350
Cdd:PRK10524 297 PLLAGMatimyeglptrpdagIWWRIVEKykvNRMF----SAPTAIRVLKKQDPallrkHDLSSLRALF--LAGEP---- 366
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 351 LDfKPLILTLGGGMAVqrPVAER-WQHMTGCHItegyglsetspVACANALDA--TEFtGTIGLPMPSTDVVIRDD-DGS 426
Cdd:PRK10524 367 LD-EPTASWISEALGV--PVIDNyWQTETGWPI-----------LAIARGVEDrpTRL-GSPGVPMYGYNVKLLNEvTGE 431
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 427 DLLLGKVGEICVRGP--------------QVMKGYWNRPEETAraimadgfFRTGDMGFMDERGYTKIVDRKKDMILVSG 492
Cdd:PRK10524 432 PCGPNEKGVLVIEGPlppgcmqtvwgdddRFVKTYWSLFGRQV--------YSTFDWGIRDADGYYFILGRTDDVINVAG 503
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 493 FNVYPNEIEEVAAEHPGVLESAAVGIPNEHSGEVVKLYVVRRDPELTEDEVKAFCAE---------RLTNYKRPREVEFR 563
Cdd:PRK10524 504 HRLGTREIEESISSHPAVAEVAVVGVKDALKGQVAVAFVVPKDSDSLADREARLALEkeimalvdsQLGAVARPARVWFV 583
|
570
....*....|....*..
gi 479816261 564 ESLPKSNVGKILRRELR 580
Cdd:PRK10524 584 SALPKTRSGKLLRRAIQ 600
|
|
| FACL_like_1 |
cd05910 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
77-518 |
6.40e-21 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341236 [Multi-domain] Cd Length: 457 Bit Score: 95.99 E-value: 6.40e-21
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 77 SYKELDDHSRALAAWLQSRGLVKGDRVAIMMPNILQY-PITFtAVLRAGFVVVNVNPLYTPRELEHQLNDAGAKALvvle 155
Cdd:cd05910 4 SFRELDERSDRIAQGLTAYGIRRGMRAVLMVPPGPDFfALTF-ALFKAGAVPVLIDPGMGRKNLKQCLQEAEPDAF---- 78
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 156 nfattvekalpsINVPnilvasmgdmlgfkghivnlvvrrvkkmvpawnipghvrfkdaLAQgraksfnpvpvqgcDIAF 235
Cdd:cd05910 79 ------------IGIP-------------------------------------------KAD--------------EPAA 89
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 236 LQYTGGTTGISKGAMLTHSNILANVEQMNLWMDVAfrnkgkPKALNfvcaLPLYHIFALtVNAMIGMK--LGARNILIP- 312
Cdd:cd05910 90 ILFTSGSTGTPKGVVYRHGTFAAQIDALRQLYGIR------PGEVD----LATFPLFAL-FGPALGLTsvIPDMDPTRPa 158
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 313 --NPRDIpsfVKELKKYPVHIFPGLNTLFNGLMNNPDFQTLDFKPLILTLGGGMAVQRPVAERWQHMT--GCHITEGYGL 388
Cdd:cd05910 159 raDPQKL---VGAIRQYGVSIVFGSPALLERVARYCAQHGITLPSLRRVLSAGAPVPIALAARLRKMLsdEAEILTPYGA 235
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 389 SETSPVAC--ANALDATEFTGT-------IGLPMPSTDVVI---------RDDDGSDLLLGKVGEICVRGPQVMKGYWNR 450
Cdd:cd05910 236 TEALPVSSigSRELLATTTAATsggagtcVGRPIPGVRVRIieiddepiaEWDDTLELPRGEIGEITVTGPTVTPTYVNR 315
|
410 420 430 440 450 460 470
....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 479816261 451 PEETARAIMADG----FFRTGDMGFMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVAAEHPGVLESAAVGI 518
Cdd:cd05910 316 PVATALAKIDDNsegfWHRMGDLGYLDDEGRLWFCGRKAHRVITTGGTLYTEPVERVFNTHPGVRRSALVGV 387
|
|
| AMP-binding_C |
pfam13193 |
AMP-binding enzyme C-terminal domain; This is a small domain that is found C terminal to ... |
499-573 |
9.18e-21 |
|
AMP-binding enzyme C-terminal domain; This is a small domain that is found C terminal to pfam00501. It has a central beta sheet core that is flanked by alpha helices.
Pssm-ID: 463804 [Multi-domain] Cd Length: 76 Bit Score: 86.44 E-value: 9.18e-21
10 20 30 40 50 60 70
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 479816261 499 EIEEVAAEHPGVLESAAVGIPNEHSGEVVKLYVVRRD-PELTEDEVKAFCAERLTNYKRPREVEFRESLPKSNVGK 573
Cdd:pfam13193 1 EVESALVSHPAVAEAAVVGVPDELKGEAPVAFVVLKPgVELLEEELVAHVREELGPYAVPKEVVFVDELPKTRSGK 76
|
|
| PRK05851 |
PRK05851 |
long-chain-fatty acid--ACP ligase MbtM; |
218-580 |
1.25e-20 |
|
long-chain-fatty acid--ACP ligase MbtM;
Pssm-ID: 180289 [Multi-domain] Cd Length: 525 Bit Score: 95.60 E-value: 1.25e-20
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 218 GRAKSFNPVPVQGcdIAFLQYTGGTTGISKGAMLTHSNILANVEQMNLWMDVAfrnkgkpKALNFVCA-LPLYH----IF 292
Cdd:PRK05851 141 NRSASLTPPDSGG--PAVLQGTAGSTGTPRTAILSPGAVLSNLRGLNARVGLD-------AATDVGCSwLPLYHdmglAF 211
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 293 ALTvNAMIGMKLGarniLIPNPrdipSFVKElkkyPVHIFPGLNTLFNGLMNNPDF------------QTLDFKPLILTL 360
Cdd:PRK05851 212 LLT-AALAGAPLW----LAPTT----AFSAS----PFRWLSWLSDSRATLTAAPNFaynligkyarrvSDVDLGALRVAL 278
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 361 GGGMAVQRPVAERWQHMTG------CHITEGYGLSE-----TSPVACANAL--DATEFTGT-------IGLPMPSTDVVI 420
Cdd:PRK05851 279 NGGEPVDCDGFERFATAMApfgfdaGAAAPSYGLAEstcavTVPVPGIGLRvdEVTTDDGSgarrhavLGNPIPGMEVRI 358
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 421 RDDDGSDLLLGK-VGEICVRGPQVMKGYWNRPeetarAIMADGFFRTGDMGFMDERGYTkIVDRKKDMILVSGFNVYPNE 499
Cdd:PRK05851 359 SPGDGAAGVAGReIGEIEIRGASMMSGYLGQA-----PIDPDDWFPTGDLGYLVDGGLV-VCGRAKELITVAGRNIFPTE 432
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 500 IEEVAAEHPGVLESA--AVGIpNEHS---GEVVKLYVVRRDPELTEDE----VKAFCAerltnyKRPREVEFRE--SLPK 568
Cdd:PRK05851 433 IERVAAQVRGVREGAvvAVGT-GEGSarpGLVIAAEFRGPDEAGARSEvvqrVASECG------VVPSDVVFVApgSLPR 505
|
410
....*....|..
gi 479816261 569 SNVGKILRRELR 580
Cdd:PRK05851 506 TSSGKLRRLAVK 517
|
|
| PLN02654 |
PLN02654 |
acetate-CoA ligase |
64-580 |
4.14e-20 |
|
acetate-CoA ligase
Pssm-ID: 215353 [Multi-domain] Cd Length: 666 Bit Score: 94.19 E-value: 4.14e-20
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 64 ANSPAFTcmgKDISYKELDDHSRALAAWLQSRGLVKGDRVAIMMPNILQYPITFTAVLRAGFVVVNVNPLYTPRELEHQL 143
Cdd:PLN02654 112 GNEPGFD---ASLTYSELLDRVCQLANYLKDVGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSAESLAQRI 188
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 144 NDAGAKALVVlenfATTVEKALPSINVPNILVASMGDMLGfKGHIVNLVV-----RRVKKMVPAWNIPGHVRFKDALAQG 218
Cdd:PLN02654 189 VDCKPKVVIT----CNAVKRGPKTINLKDIVDAALDESAK-NGVSVGICLtyenqLAMKREDTKWQEGRDVWWQDVVPNY 263
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 219 RAKSfnPVP-VQGCDIAFLQYTGGTTGISKGAMLTHSNILanveqmnLWMDVAFRN--KGKPKALNFV---CALPLYHIF 292
Cdd:PLN02654 264 PTKC--EVEwVDAEDPLFLLYTSGSTGKPKGVLHTTGGYM-------VYTATTFKYafDYKPTDVYWCtadCGWITGHSY 334
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 293 aLTVNAMIGmklGARNIL---IPNPRDIPSFVKELKKYPVHIFPGLNTLFNGLMNNPD-FQTLDFKPLILTLGGGMAVQR 368
Cdd:PLN02654 335 -VTYGPMLN---GATVLVfegAPNYPDSGRCWDIVDKYKVTIFYTAPTLVRSLMRDGDeYVTRHSRKSLRVLGSVGEPIN 410
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 369 PVAERW-QHMTG---CHITEGYGLSET-----SPVACANALDAteftGTIGLPMPSTDVVIRDDDGSDLLLGKVGEICVR 439
Cdd:PLN02654 411 PSAWRWfFNVVGdsrCPISDTWWQTETggfmiTPLPGAWPQKP----GSATFPFFGVQPVIVDEKGKEIEGECSGYLCVK 486
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 440 GP-----QVMKGYWNRPEETARAIMAdGFFRTGDMGFMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVAAEHPGVLESA 514
Cdd:PLN02654 487 KSwpgafRTLYGDHERYETTYFKPFA-GYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAA 565
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 515 AVGIPNEHSGEVVKLYVVRRDPELTEDEVKAFCAERLTN----YKRPREVEFRESLPKSNVGKILRRELR 580
Cdd:PLN02654 566 VVGIEHEVKGQGIYAFVTLVEGVPYSEELRKSLILTVRNqigaFAAPDKIHWAPGLPKTRSGKIMRRILR 635
|
|
| PRK05691 |
PRK05691 |
peptide synthase; Validated |
54-579 |
1.12e-19 |
|
peptide synthase; Validated
Pssm-ID: 235564 [Multi-domain] Cd Length: 4334 Bit Score: 94.08 E-value: 1.12e-19
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 54 DMISASCRQFANSPAFTCMGKDISYKELDDHSRALAAWLQSRGLVKGDRVAIMMPNILQYPITFTAVLRAGFVVVNVNPL 133
Cdd:PRK05691 1135 ELLNEQARQTPERIALVWDGGSLDYAELHAQANRLAHYLRDKGVGPDVCVAIAAERSPQLLVGLLAILKAGGAYVPLDPD 1214
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 134 YTPRELEHQLNDAGAKALVVlenfATTVEKALPSinVPNILVASMgDMLgfkgHIVNLVVRrvkkmVPAWNIPGHvrfkd 213
Cdd:PRK05691 1215 YPAERLAYMLADSGVELLLT----QSHLLERLPQ--AEGVSAIAL-DSL----HLDSWPSQ-----APGLHLHGD----- 1273
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 214 alaqgraksfnpvpvqgcDIAFLQYTGGTTGISKGAMLTHSnilANVEQMNlWMDVAFRNKGK----PKA-LNF-----V 283
Cdd:PRK05691 1274 ------------------NLAYVIYTSGSTGQPKGVGNTHA---ALAERLQ-WMQATYALDDSdvlmQKApISFdvsvwE 1331
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 284 CALPLyhifaLTvnamigmklGARNILIP-----NPRDIPSFVKELKKYPVHIFPGLNTLFnglMNNPDFQtlDFKPLIL 358
Cdd:PRK05691 1332 CFWPL-----IT---------GCRLVLAGpgehrDPQRIAELVQQYGVTTLHFVPPLLQLF---IDEPLAA--ACTSLRR 1392
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 359 TLGGGMAVQRPVAER-WQHMTGCHITEGYGLSETspvacanALDATEFT--------GTIGLPMPSTDVVIRDDDGSDLL 429
Cdd:PRK05691 1393 LFSGGEALPAELRNRvLQRLPQVQLHNRYGPTET-------AINVTHWQcqaedgerSPIGRPLGNVLCRVLDAELNLLP 1465
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 430 LGKVGEICVRGPQVMKGYWNRPEETARAIMADGF-------FRTGDMGFMDERGYTKIVDRKKDMILVSGFNVYPNEIEE 502
Cdd:PRK05691 1466 PGVAGELCIGGAGLARGYLGRPALTAERFVPDPLgedgarlYRTGDRARWNADGALEYLGRLDQQVKLRGFRVEPEEIQA 1545
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 479816261 503 VAAEHPGVlESAAVGIPNEHSG-EVVKLYVVRRDPELTEDEVKAFCAERLTNYKRPREVEFRESLPKSNVGKILRREL 579
Cdd:PRK05691 1546 RLLAQPGV-AQAAVLVREGAAGaQLVGYYTGEAGQEAEAERLKAALAAELPEYMVPAQLIRLDQMPLGPSGKLDRRAL 1622
|
|
| A_NRPS_ACVS-like |
cd17648 |
N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase; This family contains ACV ... |
68-579 |
1.37e-19 |
|
N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase; This family contains ACV synthetase (ACVS, EC 6.3.2.26; also known as N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase or delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine synthetase) is involved in medically important antibiotic biosynthesis. ACV synthetase is active in an early step in the penicillin G biosynthesis pathway which involves the formation of the tripeptide 6-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine (ACV); each of the constituent amino acids of the tripeptide ACV are activated as aminoacyl-adenylates with peptide bonds formed through the participation of amino acid thioester intermediates. ACV is then cyclized by the action of isopenicillin N synthase.
Pssm-ID: 341303 [Multi-domain] Cd Length: 453 Bit Score: 91.69 E-value: 1.37e-19
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 68 AFTCMGKDISYKELDDHSRALAAWLQSRGLVKGD-RVAIMMPNILQYPITFTAVLRAGFVVVNVNPLYTPRELEHQLNDA 146
Cdd:cd17648 5 AVVYGDKRLTYRELNERANRLAHYLLSVAEIRPDdLVGLVLDKSELMIIAILAVWKAGAAYVPIDPSYPDERIQFILEDT 84
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 147 GAKALVvlenfaTTVEkalpsinvpnilvasmgdmlgfkghivnlvvrrvkkmvpawnipghvrfkdalaqgraksfnpv 226
Cdd:cd17648 85 GARVVI------TNST---------------------------------------------------------------- 94
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 227 pvqgcDIAFLQYTGGTTGISKGAMLTHSNILanveqmNLWMDVAFRNKGKPKALNFVCALPLYhIFALTVNAMIGMKLGA 306
Cdd:cd17648 95 -----DLAYAIYTSGTTGKPKGVLVEHGSVV------NLRTSLSERYFGRDNGDEAVLFFSNY-VFDFFVEQMTLALLNG 162
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 307 RNILIPNPR---DIPSFVKELKKYPVHIFPGLNTLFNGLmnnpDFQTLDfkPLILTLGGGMAVQRPVAERWQHMTGCHIT 383
Cdd:cd17648 163 QKLVVPPDEmrfDPDRFYAYINREKVTYLSGTPSVLQQY----DLARLP--HLKRVDAAGEEFTAPVFEKLRSRFAGLII 236
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 384 EGYGLSETSPVACANALDATE-FTGTIGLPMPSTDVVIRDDDGSDLLLGKVGEICVRGPQVMKGYWNRPEETARAIMADG 462
Cdd:cd17648 237 NAYGPTETTVTNHKRFFPGDQrFDKSLGRPVRNTKCYVLNDAMKRVPVGAVGELYLGGDGVARGYLNRPELTAERFLPNP 316
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 463 F--------------FRTGDMGFMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVAAEHPGVLESAAVgiPNEHSGE--- 525
Cdd:cd17648 317 FqteqerargrnarlYKTGDLVRWLPSGELEYLGRNDFQVKIRGQRIEPGEVEAALASYPGVRECAVV--AKEDASQaqs 394
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 526 -----VVKLYVVrrDPE-LTEDEVKAFCAERLTNYKRPREVEFRESLPKSNVGKILRREL 579
Cdd:cd17648 395 riqkyLVGYYLP--EPGhVPESDLLSFLRAKLPRYMVPARLVRLEGIPVTINGKLDVRAL 452
|
|
| PLN02861 |
PLN02861 |
long-chain-fatty-acid-CoA ligase |
76-519 |
1.07e-18 |
|
long-chain-fatty-acid-CoA ligase
Pssm-ID: 178452 [Multi-domain] Cd Length: 660 Bit Score: 89.90 E-value: 1.07e-18
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 76 ISYKELDDHSRALAAWLQSRGLVKGDRVAIMMPNILQYPITFTAVLRAGFVVVnvnPLYT---PRELEHQLNDAGAKALV 152
Cdd:PLN02861 78 LTYKEVYDAAIRIGSAIRSRGVNPGDRCGIYGSNCPEWIIAMEACNSQGITYV---PLYDtlgANAVEFIINHAEVSIAF 154
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 153 VLENFATTVEKALPSINVPNILVASMGDmlgfkghIVNLVVRRVKKM---VPAWNipghvrfkdALAQGRAKSFNPVPVQ 229
Cdd:PLN02861 155 VQESKISSILSCLPKCSSNLKTIVSFGD-------VSSEQKEEAEELgvsCFSWE---------EFSLMGSLDCELPPKQ 218
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 230 GCDIAFLQYTGGTTGISKGAMLTHSNILANV---EQMNLWMDvafrnKGKPKALNFVCALPLYHIF-------ALTVNAM 299
Cdd:PLN02861 219 KTDICTIMYTSGTTGEPKGVILTNRAIIAEVlstDHLLKVTD-----RVATEEDSYFSYLPLAHVYdqvietyCISKGAS 293
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 300 IGMKLGARNILIPNPRDI-PSFVKELKKYPVHIFPGLN-----------TLFNGLMN---------------NPDFQTLD 352
Cdd:PLN02861 294 IGFWQGDIRYLMEDVQALkPTIFCGVPRVYDRIYTGIMqkissggmlrkKLFDFAYNyklgnlrkglkqeeaSPRLDRLV 373
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 353 FKPLILTLGG-------GMAVQRPVAERWQHMTGCH-ITEGYGLSETSPVACANALDATEFTGTIGLPMPSTDVVIRD-- 422
Cdd:PLN02861 374 FDKIKEGLGGrvrlllsGAAPLPRHVEEFLRVTSCSvLSQGYGLTESCGGCFTSIANVFSMVGTVGVPMTTIEARLESvp 453
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 423 DDGSDLLLG-KVGEICVRGPQVMKGYWNRPEETaRAIMADGFFRTGDMGFMDERGYTKIVDRKKDMILVSgfnvypnEIE 501
Cdd:PLN02861 454 EMGYDALSDvPRGEICLRGNTLFSGYHKRQDLT-EEVLIDGWFHTGDIGEWQPNGAMKIIDRKKNIFKLS-------QGE 525
|
490
....*....|....*...
gi 479816261 502 EVAAEhpgVLESAAVGIP 519
Cdd:PLN02861 526 YVAVE---NLENTYSRCP 540
|
|
| PRK05691 |
PRK05691 |
peptide synthase; Validated |
205-579 |
1.39e-18 |
|
peptide synthase; Validated
Pssm-ID: 235564 [Multi-domain] Cd Length: 4334 Bit Score: 90.61 E-value: 1.39e-18
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 205 IPGHVRFK----DALAQGRAKSFNPVPVQGCD-IAFLQYTGGTTGISKGAMLTHSNILANVEQMNLWMDVAFRNkgkpka 279
Cdd:PRK05691 3838 LGCANRPRllvwEEVQAGEVASHNPGIYSGPDnLAYVIYTSGSTGLPKGVMVEQRGMLNNQLSKVPYLALSEAD------ 3911
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 280 lnfVCALPLYHIFALTVNAMIGMKL-GARNILIPN--PRDIPSFVKELKKYPVHIFPGLNTLFNGLMNNpDFQTLDFKPL 356
Cdd:PRK05691 3912 ---VIAQTASQSFDISVWQFLAAPLfGARVEIVPNaiAHDPQGLLAHVQAQGITVLESVPSLIQGMLAE-DRQALDGLRW 3987
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 357 ILTLGGGMAVQrpVAERW-QHMTGCHITEGYGLSETSPVACANALDATEFTGT---IGLPMPSTDVVIRDDDGSDLLLGK 432
Cdd:PRK05691 3988 MLPTGEAMPPE--LARQWlQRYPQIGLVNAYGPAECSDDVAFFRVDLASTRGSylpIGSPTDNNRLYLLDEALELVPLGA 4065
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 433 VGEICVRGPQVMKGYWNRPEETARAIMADGF-------FRTGDMGFMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVAA 505
Cdd:PRK05691 4066 VGELCVAGTGVGRGYVGDPLRTALAFVPHPFgapgerlYRTGDLARRRSDGVLEYVGRIDHQVKIRGYRIELGEIEARLH 4145
|
330 340 350 360 370 380 390
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 479816261 506 EHPGVLEsAAVGIPNEHSGEVVKLYVVRRDPELTEDEVKAFCAERLT----NYKRPREVEFRESLPKSNVGKILRREL 579
Cdd:PRK05691 4146 EQAEVRE-AAVAVQEGVNGKHLVGYLVPHQTVLAQGALLERIKQRLRaelpDYMVPLHWLWLDRLPLNANGKLDRKAL 4222
|
|
| PLN02387 |
PLN02387 |
long-chain-fatty-acid-CoA ligase family protein |
76-488 |
1.29e-17 |
|
long-chain-fatty-acid-CoA ligase family protein
Pssm-ID: 215217 [Multi-domain] Cd Length: 696 Bit Score: 86.71 E-value: 1.29e-17
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 76 ISYKELDDHSRALAAWLQSRGLVKGDRVAIMMPNILQYPITFTAVLRAGFVVVNVNPLYTPRELEHQLNdagakalvvlE 155
Cdd:PLN02387 107 ITYGQVFERVCNFASGLVALGHNKEERVAIFADTRAEWLIALQGCFRQNITVVTIYASLGEEALCHSLN----------E 176
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 156 NFATTV---EKALPSInvpnilvASMGDMLGfkghivnlVVRRVKKM-------------VPAWNIpghVRFKDALAQGR 219
Cdd:PLN02387 177 TEVTTVicdSKQLKKL-------IDISSQLE--------TVKRVIYMddegvdsdsslsgSSNWTV---SSFSEVEKLGK 238
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 220 AKSFNPVPVQGCDIAFLQYTGGTTGISKGAMLTHSNILANVEQMnlwMDVAfrnkgkPKaLN----FVCALPLYHIFAL- 294
Cdd:PLN02387 239 ENPVDPDLPSPNDIAVIMYTSGSTGLPKGVMMTHGNIVATVAGV---MTVV------PK-LGkndvYLAYLPLAHILELa 308
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 295 ------TVNAMIG-------------MKLGAR---NILIPN-----PRdIPSFVKE--LKKypVHIFPGL-NTLFN---- 340
Cdd:PLN02387 309 aesvmaAVGAAIGygspltltdtsnkIKKGTKgdaSALKPTlmtavPA-ILDRVRDgvRKK--VDAKGGLaKKLFDiayk 385
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 341 ---GLMNNPDF----------QTLDFKPLILTLGG-------GMAVQRPVAERWQHMT-GCHITEGYGLSETspvaCANA 399
Cdd:PLN02387 386 rrlAAIEGSWFgawglekllwDALVFKKIRAVLGGrirfmlsGGAPLSGDTQRFINIClGAPIGQGYGLTET----CAGA 461
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 400 lDATEF----TGTIGLPMPSTDVVIRD-DDGSDLLLGKV---GEICVRGPQVMKGYWNRPEETARAIMADG----FFRTG 467
Cdd:PLN02387 462 -TFSEWddtsVGRVGPPLPCCYVKLVSwEEGGYLISDKPmprGEIVIGGPSVTLGYFKNQEKTDEVYKVDErgmrWFYTG 540
|
490 500
....*....|....*....|.
gi 479816261 468 DMGFMDERGYTKIVDRKKDMI 488
Cdd:PLN02387 541 DIGQFHPDGCLEIIDRKKDIV 561
|
|
| Dip2 |
cd05905 |
Disco-interacting protein 2 (Dip2); Dip2 proteins show sequence similarity to other members of ... |
68-542 |
3.58e-17 |
|
Disco-interacting protein 2 (Dip2); Dip2 proteins show sequence similarity to other members of the adenylate forming enzyme family, including insect luciferase, acetyl CoA ligases and the adenylation domain of nonribosomal peptide synthetases (NRPS). However, its function may have diverged from other members of the superfamily. In mouse embryo, Dip2 homolog A plays an important role in the development of both vertebrate and invertebrate nervous systems. Dip2A appears to regulate cell growth and the arrangement of cells in organs. Biochemically, Dip2A functions as a receptor of FSTL1, an extracellular glycoprotein, and may play a role as a cardiovascular protective agent.
Pssm-ID: 341231 [Multi-domain] Cd Length: 571 Bit Score: 84.71 E-value: 3.58e-17
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 68 AFTCM------GKDISYKELddHSRA--LAAWLQSRGLVK-GDRVAIMMPNILQYPITFTAVLRAGFVVVNVNPLYTPRE 138
Cdd:cd05905 1 AYTLLdskgkeATTLTWGKL--LSRAekIAAVLQKKVGLKpGDRVALMYPDPLDFVAAFYGCLYAGVVPIPIEPPDISQQ 78
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 139 LEHQLNDAGAKalVVLENFATTveKALPSINvpnilvasmgDMLGFKGHIVNLVVrrVKKMVPAWNIPGHVRFKDALAQG 218
Cdd:cd05905 79 LGFLLGTCKVR--VALTVEACL--KGLPKKL----------LKSKTAAEIAKKKG--WPKILDFVKIPKSKRSKLKKWGP 142
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 219 RAKSFNPvpvqgcDIAFLQYTGGTTG------ISKGAMLTHSNILAnvEQMNLwmdvafrNKGKPkalnFVCALPLYHIF 292
Cdd:cd05905 143 HPPTRDG------DTAYIEYSFSSDGslsgvaVSHSSLLAHCRALK--EACEL-------YESRP----LVTVLDFKSGL 203
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 293 ALTVNAMIGMKLGARNILIP------NPrdiPSFVKELKKYPVH-IFPGLNTLFNGLMN-----------NPDFQTL--- 351
Cdd:cd05905 204 GLWHGCLLSVYSGHHTILIPpelmktNP---LLWLQTLSQYKVRdAYVKLRTLHWCLKDlsstlaslknrDVNLSSLrmc 280
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 352 ------------------DFKPL------ILTLGGGMAVQRPVaerWQHMTGCHITEGY----GLSETSP-VACANALDA 402
Cdd:cd05905 281 mvpcenrprisscdsflkLFQTLglspraVSTEFGTRVNPFIC---WQGTSGPEPSRVYldmrALRHGVVrLDERDKPNS 357
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 403 TeftgTI---GLPMPSTDVVIRDDDGSDLLL-GKVGEICVRGPQVMKGYWNRPEET------------ARAIMADGFFRT 466
Cdd:cd05905 358 L----PLqdsGKVLPGAQVAIVNPETKGLCKdGEIGEIWVNSPANASGYFLLDGETndtfkvfpstrlSTGITNNSYART 433
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 467 GDMGF----------MDERGYTKIVDRKKDMILVSGFNVYPNEIEE-VAAEHPGVLESAAVGIpnehSGEVVklyVVRRD 535
Cdd:cd05905 434 GLLGFlrptkctdlnVEEHDLLFVVGSIDETLEVRGLRHHPSDIEAtVMRVHPYRGRCAVFSI----TGLVV---VVAEQ 506
|
....*..
gi 479816261 536 PELTEDE 542
Cdd:cd05905 507 PPGSEEE 513
|
|
| PTZ00237 |
PTZ00237 |
acetyl-CoA synthetase; Provisional |
226-576 |
1.01e-16 |
|
acetyl-CoA synthetase; Provisional
Pssm-ID: 240325 [Multi-domain] Cd Length: 647 Bit Score: 83.64 E-value: 1.01e-16
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 226 VPVQGCDIAFLQYTGGTTGISKGAMLTHSNILANVeqmnLWMDVAFRNKGKPKAL--NFVCALPLYHIF---ALTVNAMI 300
Cdd:PTZ00237 249 VPVESSHPLYILYTSGTTGNSKAVVRSNGPHLVGL----KYYWRSIIEKDIPTVVfsHSSIGWVSFHGFlygSLSLGNTF 324
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 301 GMKLGArnilIPNPRDIPS-FVKELKKYPV-HIFPGLNTLFNGLMNNPDFQTL----DFKPLILTLGGGMAVQRPVAERW 374
Cdd:PTZ00237 325 VMFEGG----IIKNKHIEDdLWNTIEKHKVtHTLTLPKTIRYLIKTDPEATIIrskyDLSNLKEIWCGGEVIEESIPEYI 400
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 375 QHMTGCHITEGYGLSETspvACANALDATEFT---GTIGLPMPSTDVVIRDDDGSDLLLGKVGEICVRGPQ----VMKGY 447
Cdd:PTZ00237 401 ENKLKIKSSRGYGQTEI---GITYLYCYGHINipyNATGVPSIFIKPSILSEDGKELNVNEIGEVAFKLPMppsfATTFY 477
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 448 WNrpEETARAIMAD--GFFRTGDMGFMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVAAEHPGVLESAAVGIPNEHSGE 525
Cdd:PTZ00237 478 KN--DEKFKQLFSKfpGYYNSGDLGFKDENGYYTIVSRSDDQIKISGNKVQLNTIETSILKHPLVLECCSIGIYDPDCYN 555
|
330 340 350 360 370
....*....|....*....|....*....|....*....|....*....|....*....
gi 479816261 526 V-VKLYVVRRDPELT-------EDEVKAFCAERLTNYKRPREVEFRESLPKSNVGKILR 576
Cdd:PTZ00237 556 VpIGLLVLKQDQSNQsidlnklKNEINNIITQDIESLAVLRKIIIVNQLPKTKTGKIPR 614
|
|
| FATP_chFAT1_like |
cd05937 |
Uncharacterized subfamily of bifunctional fatty acid transporter/very-long-chain acyl-CoA ... |
232-581 |
4.91e-15 |
|
Uncharacterized subfamily of bifunctional fatty acid transporter/very-long-chain acyl-CoA synthetase in fungi; Fatty acid transport protein (FATP) transports long-chain or very-long-chain fatty acids across the plasma membrane. FATPs also have fatty acid CoA synthetase activity, thus playing dual roles as fatty acid transporters and its activation enzymes. FATPs are the key players in the trafficking of exogenous fatty acids into the cell and in intracellular fatty acid homeostasis. Members of this family are fungal FATPs, including FAT1 from Cochliobolus heterostrophus.
Pssm-ID: 341260 [Multi-domain] Cd Length: 468 Bit Score: 77.86 E-value: 4.91e-15
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 232 DIAFLQYTGGTTGISKGAMLTHSNILANVEQMNLWMDVAFRNKgkpkalnFVCALPLYHIFALTVNAM----------IG 301
Cdd:cd05937 88 DPAILIYTSGTTGLPKAAAISWRRTLVTSNLLSHDLNLKNGDR-------TYTCMPLYHGTAAFLGACnclmsggtlaLS 160
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 302 MKLGARNI---LIPNPRDIPSFVKELKKYpvhifpglntlfngLMNNPDFQTLDFKPLILTLGGGMavqRP-VAERWQHM 377
Cdd:cd05937 161 RKFSASQFwkdVRDSGATIIQYVGELCRY--------------LLSTPPSPYDRDHKVRVAWGNGL---RPdIWERFRER 223
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 378 TGC-HITEGYglSETSPVACANALDATEFT-GTIGL--------------PM---PSTDVVIRDDDGS---DLLLGKVGE 435
Cdd:cd05937 224 FNVpEIGEFY--AATEGVFALTNHNVGDFGaGAIGHhglirrwkfenqvvLVkmdPETDDPIRDPKTGfcvRAPVGEPGE 301
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 436 ICVRGPQVMK----GYWNRPEETARAIMADGF------FRTGDMGFMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVAA 505
Cdd:cd05937 302 MLGRVPFKNReafqGYLHNEDATESKLVRDVFrkgdiyFRTGDLLRQDADGRWYFLDRLGDTFRWKSENVSTTEVADVLG 381
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 506 EHPGVLESAAVGI--PNeHSGEV----VKLYVVRRDP-ELTEDEVKAFCAERLTNYKRPREVEFRESLPKSNVGKILRRE 578
Cdd:cd05937 382 AHPDIAEANVYGVkvPG-HDGRAgcaaITLEESSAVPtEFTKSLLASLARKNLPSYAVPLFLRLTEEVATTDNHKQQKGV 460
|
...
gi 479816261 579 LRD 581
Cdd:cd05937 461 LRD 463
|
|
| PRK06334 |
PRK06334 |
long chain fatty acid--[acyl-carrier-protein] ligase; Validated |
76-529 |
3.64e-14 |
|
long chain fatty acid--[acyl-carrier-protein] ligase; Validated
Pssm-ID: 180533 [Multi-domain] Cd Length: 539 Bit Score: 75.24 E-value: 3.64e-14
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 76 ISYKELDDHSRALAAWLQSrglVKGDRVAIMMPNILQYPITFTAVLRAGFVVVNVNPLYTPRELEHQLNDAGAKALVVLE 155
Cdd:PRK06334 46 LSYNQVRKAVIALATKVSK---YPDQHIGIMMPASAGAYIAYFATLLSGKIPVMINWSQGLREVTACANLVGVTHVLTSK 122
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 156 NFattVEKalpsinvpniLVASMGDMLGFKGHIVNLvvRRVKKMVPAWNiPGHVRFKDALA-QGRAKSFNPVPVQGCDIA 234
Cdd:PRK06334 123 QL---MQH----------LAQTHGEDAEYPFSLIYM--EEVRKELSFWE-KCRIGIYMSIPfEWLMRWFGVSDKDPEDVA 186
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 235 FLQYTGGTTGISKGAMLTHSNILANVEQMNLWMDvafrnkgkPKALNFVCA-LPLYHIFALTVNAMIGMKLGARNILIPN 313
Cdd:PRK06334 187 VILFTSGTEKLPKGVPLTHANLLANQRACLKFFS--------PKEDDVMMSfLPPFHAYGFNSCTLFPLLSGVPVVFAYN 258
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 314 PRDIPSFVKELKKYPVHIFPGLNTLFNGLMNNPDFQTLDFKPLILTLGGGMAVQRPVAERWQHmTGCHIT--EGYGLSET 391
Cdd:PRK06334 259 PLYPKKIVEMIDEAKVTFLGSTPVFFDYILKTAKKQESCLPSLRFVVIGGDAFKDSLYQEALK-TFPHIQlrQGYGTTEC 337
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 392 SPVACANALDATEFTGTIGLPMPSTDV-VIRDDDGSDLLLGKVGEICVRGPQVMKGYW-NRPEETARAIMADGFFRTGDM 469
Cdd:PRK06334 338 SPVITINTVNSPKHESCVGMPIRGMDVlIVSEETKVPVSSGETGLVLTRGTSLFSGYLgEDFGQGFVELGGETWYVTGDL 417
|
410 420 430 440 450 460
....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 479816261 470 GFMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVAAEHPGVLES------AAVGIPnehsGEVVKL 529
Cdd:PRK06334 418 GYVDRHGELFLKGRLSRFVKIGAEMVSLEALESILMEGFGQNAAdhagplVVCGLP----GEKVRL 479
|
|
| PTZ00342 |
PTZ00342 |
acyl-CoA synthetase; Provisional |
233-491 |
2.71e-13 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 240370 [Multi-domain] Cd Length: 746 Bit Score: 72.83 E-value: 2.71e-13
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 233 IAFLQYTGGTTGISKGAMLTHSNILANVEQMNLWmdvAFRNKGKPKA-LNFvcaLPLYHIFALTVNAMIGMKLGARNILi 311
Cdd:PTZ00342 306 ITSIVYTSGTSGKPKGVMLSNKNLYNTVVPLCKH---SIFKKYNPKThLSY---LPISHIYERVIAYLSFMLGGTINIW- 378
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 312 pnPRDIPSFVKELKKYPVHIFPGLNTLFNGLMNN--------PDFQTLDFKPLI-------------------------- 357
Cdd:PTZ00342 379 --SKDINYFSKDIYNSKGNILAGVPKVFNRIYTNimteinnlPPLKRFLVKKILslrksnnnggfskflegithisskik 456
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 358 --------LTLGGGMAVQRPVAERWQHMTGCHITEGYGLSETS-PVACANALDATefTGTIGLPM-PSTDVVIRD---DD 424
Cdd:PTZ00342 457 dkvnpnleVILNGGGKLSPKIAEELSVLLNVNYYQGYGLTETTgPIFVQHADDNN--TESIGGPIsPNTKYKVRTwetYK 534
|
250 260 270 280 290 300
....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 479816261 425 GSDLLlgKVGEICVRGPQVMKGYWNRPEETARAIMADGFFRTGDMGFMDERGYTKIVDRKKDMILVS 491
Cdd:PTZ00342 535 ATDTL--PKGELLIKSDSIFSGYFLEKEQTKNAFTEDGYFKTGDIVQINKNGSLTFLDRSKGLVKLS 599
|
|
| AFD_CAR-like |
cd17632 |
adenylation domain of carboxylic acid reductase (CAR); This family contains the adenylation ... |
76-491 |
5.22e-12 |
|
adenylation domain of carboxylic acid reductase (CAR); This family contains the adenylation domain of carboxylic acid reductase enzymes (CARs), and performs an equivalent function to that of the ANL superfamily of adenylating enzymes. It takes a carboxylic acid substrate and ATP, and produces an AMP-acyl phosphoester intermediate, releasing pyrophosphate. Kinetic analysis using various substrates shows that this enzyme has a broad but similar substrate specificity, preferring electron-rich acids. This suggests that attack by the carboxylate on the alpha-phosphate of adenosine triphosphate (ATP) is the step that determines the substrate specificity and reaction kinetics. CAR is an important enzyme for use as a biocatalyst providing regiospecific route to aldehydes from their respective carboxylic acids.
Pssm-ID: 341287 [Multi-domain] Cd Length: 588 Bit Score: 68.64 E-value: 5.22e-12
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 76 ISYKELDDHSRALAAWLQSRGLVK-GDRVAIMMPNILQYPITFTAVLRAGFVVVnvnPLYT---PRELEHQLNDAGAKAL 151
Cdd:cd17632 68 ITYAELWERVGAVAAAHDPEQPVRpGDFVAVLGFTSPDYATVDLALTRLGAVSV---PLQAgasAAQLAPILAETEPRLL 144
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 152 VV-LENFATTVEKALPSINVPNILVasmgdmlgFKGH----IVNLVVRRVKKMVPAWNIPGHVrfkDALAQGRAKSFNPV 226
Cdd:cd17632 145 AVsAEHLDLAVEAVLEGGTPPRLVV--------FDHRpevdAHRAALESARERLAAVGIPVTT---LTLIAVRGRDLPPA 213
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 227 PVQGCD-----IAFLQYTGGTTGISKGAMLTHSNIlanveqMNLWMDVAFRNKGKPKA---LNFvcaLPLYHIfaltvna 298
Cdd:cd17632 214 PLFRPEpdddpLALLIYTSGSTGTPKGAMYTERLV------ATFWLKVSSIQDIRPPAsitLNF---MPMSHI------- 277
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 299 migmklGARNILIPNprdipsfvkeLKKYPVHIF---PGLNTLFNGL----------------MNNPDFQTLDFKPLIL- 358
Cdd:cd17632 278 ------AGRISLYGT----------LARGGTAYFaaaSDMSTLFDDLalvrptelflvprvcdMLFQRYQAELDRRSVAg 341
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 359 -----------------TLGG----GMAVQRPVAERWQ----HMTGCHITEGYGLSETSPVAcanaLDateftGTIGLPm 413
Cdd:cd17632 342 adaetlaervkaelrerVLGGrllaAVCGSAPLSAEMKafmeSLLDLDLHDGYGSTEAGAVI----LD-----GVIVRP- 411
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 414 PSTDVVIRDDDGsdllLGKV--------GEICVRGPQVMKGYWNRPEETARAIMADGFFRTGDMgfMDERGYTKI--VDR 483
Cdd:cd17632 412 PVLDYKLVDVPE----LGYFrtdrphprGELLVKTDTLFPGYYKRPEVTAEVFDEDGFYRTGDV--MAELGPDRLvyVDR 485
|
....*...
gi 479816261 484 KKDMILVS 491
Cdd:cd17632 486 RNNVLKLS 493
|
|
| PRK09029 |
PRK09029 |
O-succinylbenzoic acid--CoA ligase; Provisional |
62-581 |
3.49e-11 |
|
O-succinylbenzoic acid--CoA ligase; Provisional
Pssm-ID: 236363 [Multi-domain] Cd Length: 458 Bit Score: 65.66 E-value: 3.49e-11
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 62 QFANSPAFTCMGKDISYKELDDHSRALAAWLQSRGLVKGDRVAIMMPNILQYPITFTAVLRAGFVVVNVNPlYTPRELEH 141
Cdd:PRK09029 15 VRPQAIALRLNDEVLTWQQLCARIDQLAAGFAQQGVVEGSGVALRGKNSPETLLAYLALLQCGARVLPLNP-QLPQPLLE 93
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 142 QLndagakalvvlenfattvekaLPSINVPNILVASmgdmlGFKgHIVNLVVRRVKKMVPAwnipghvrfkdalaqgrak 221
Cdd:PRK09029 94 EL---------------------LPSLTLDFALVLE-----GEN-TFSALTSLHLQLVEGA------------------- 127
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 222 sfNPVPVQGCDIAFLQYTGGTTGISKGAMLTHSNILANVEQMNLWMDvafrnkgkpkalnFVCA------LPLYHIFA-- 293
Cdd:PRK09029 128 --HAVAWQPQRLATMTLTSGSTGLPKAAVHTAQAHLASAEGVLSLMP-------------FTAQdswllsLPLFHVSGqg 192
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 294 -----LTVNAMigMKLGARNILipnPRDI-----PSFV-KELKKypvhifpglntlfngLMNNPdfqtldFKPLILT--- 359
Cdd:PRK09029 193 ivwrwLYAGAT--LVVRDKQPL---EQALagcthASLVpTQLWR---------------LLDNR------SEPLSLKavl 246
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 360 LGGGMAvqrPV-----AERWQHMTGChiteGYGLSETSPVACANALDATeftGTIGLPMPSTDVVIRDDdgsdlllgkvg 434
Cdd:PRK09029 247 LGGAAI---PVelteqAEQQGIRCWC----GYGLTEMASTVCAKRADGL---AGVGSPLPGREVKLVDG----------- 305
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 435 EICVRGPQVMKGYWnRPEETARAIMADGFFRTGDMGFMDErGYTKIVDRKKDMILVSGFNVYPNEIEEVAAEHPGVLESA 514
Cdd:PRK09029 306 EIWLRGASLALGYW-RQGQLVPLVNDEGWFATRDRGEWQN-GELTILGRLDNLFFSGGEGIQPEEIERVINQHPLVQQVF 383
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 479816261 515 AVGIPNEHSGE--VVklyVVRRDPELTEDEVKAFCAERLTNYKRPreVEFrESLPKS--NVG-KILRRELRD 581
Cdd:PRK09029 384 VVPVADAEFGQrpVA---VVESDSEAAVVNLAEWLQDKLARFQQP--VAY-YLLPPElkNGGiKISRQALKE 449
|
|
| PaaK |
COG1541 |
Phenylacetate-coenzyme A ligase PaaK, adenylate-forming domain family [Coenzyme transport and ... |
370-576 |
9.05e-09 |
|
Phenylacetate-coenzyme A ligase PaaK, adenylate-forming domain family [Coenzyme transport and metabolism];
Pssm-ID: 441150 [Multi-domain] Cd Length: 423 Bit Score: 57.85 E-value: 9.05e-09
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 370 VAERWqhmtGCHITEGYGLSETSPVAcanaldATEFTGTIGLPMPSTDV---VIRDDDGSDLLLGKVGEICVrgpqvmkg 446
Cdd:COG1541 223 IEERW----GIKAYDIYGLTEVGPGV------AYECEAQDGLHIWEDHFlveIIDPETGEPVPEGEEGELVV-------- 284
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 447 ywnrpeeTAraIMADGF----FRTGDMG-FMDER---GYT-----KIVDRKKDMILVSGFNVYPNEIEEVAAEHPGVLES 513
Cdd:COG1541 285 -------TT--LTKEAMplirYRTGDLTrLLPEPcpcGRThprigRILGRADDMLIIRGVNVFPSQIEEVLLRIPEVGPE 355
|
170 180 190 200 210 220
....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 479816261 514 AAVGIPNEHSGEVVKLyVVRRDPELTEDEVKAFCAERLTNYKRPR-EVEFRE--SLPKSnVGKILR 576
Cdd:COG1541 356 YQIVVDREGGLDELTV-RVELAPGASLEALAEAIAAALKAVLGLRaEVELVEpgSLPRS-EGKAKR 419
|
|
| PRK03584 |
PRK03584 |
acetoacetate--CoA ligase; |
74-257 |
7.50e-08 |
|
acetoacetate--CoA ligase;
Pssm-ID: 235134 [Multi-domain] Cd Length: 655 Bit Score: 55.19 E-value: 7.50e-08
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 74 KDISYKELDDHSRALAAWLQSRGLVKGDRVAIMMPNILQYPITFTAVLRAGFVVVNVNPlytprelehqlnDAGAKAlvV 153
Cdd:PRK03584 113 RELSWAELRRQVAALAAALRALGVGPGDRVAAYLPNIPETVVAMLATASLGAIWSSCSP------------DFGVQG--V 178
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 154 LENFAtTVEkalpsinvPNILVASMG--------DMLGFKGHIVN--------LVVRRVKKMVPAWNIPGHVRFKDALAQ 217
Cdd:PRK03584 179 LDRFG-QIE--------PKVLIAVDGyryggkafDRRAKVAELRAalpslehvVVVPYLGPAAAAAALPGALLWEDFLAP 249
|
170 180 190 200
....*....|....*....|....*....|....*....|
gi 479816261 218 GRAKSFNPVPVQGCDIAFLQYTGGTTGISKGAMLTHSNIL 257
Cdd:PRK03584 250 AEAAELEFEPVPFDHPLWILYSSGTTGLPKCIVHGHGGIL 289
|
|
| A_NRPS_acs4 |
cd17654 |
acyl-CoA synthetase family member 4; This family of the adenylation (A) domain of nonribosomal ... |
227-579 |
8.45e-08 |
|
acyl-CoA synthetase family member 4; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) contains acyl-CoA synthethase family member 4, also known as 2-aminoadipic 6-semialdehyde dehydrogenase or aminoadipate-semialdehyde dehydrogenase, most of which are uncharacterized. Acyl-CoA synthetase catalyzes the initial reaction in fatty acid metabolism, by forming a thioester with CoA. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341309 [Multi-domain] Cd Length: 449 Bit Score: 54.79 E-value: 8.45e-08
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 227 PVQGCdIAFLQYTGGTTGISKGAMLTHSNILANVEQmnlwmdvaFRNKGKPKALNFVCALPLYHIFALTVNAMIGMKLGA 306
Cdd:cd17654 115 RTDEC-LAYVIHTSGTTGTPKIVAVPHKCILPNIQH--------FRSLFNITSEDILFLTSPLTFDPSVVEIFLSLSSGA 185
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 307 RNILIPN-----PRDIPSFVKELKKYPV-HIFPglnTLFNGLMNNPDFQTL--DFKPL-ILTLGGGMAVQRPVAERWQHM 377
Cdd:cd17654 186 TLLIVPTsvkvlPSKLADILFKRHRITVlQATP---TLFRRFGSQSIKSTVlsATSSLrVLALGGEPFPSLVILSSWRGK 262
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 378 -TGCHITEGYGLSETSPVACANALDATEFTGTIGLPMPSTDVVIRDDDGSD----LLLGKVGEICvrgpqVMKGYWNRPE 452
Cdd:cd17654 263 gNRTRIFNIYGITEVSCWALAYKVPEEDSPVQLGSPLLGTVIEVRDQNGSEgtgqVFLGGLNRVC-----ILDDEVTVPK 337
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 453 ETARAimadgffrTGDmgFMD-ERGYTKIVDRKKDMILVSGFNVYPNEIEEVAAEHPGVlESAAVGIPNEHsgevvKLYV 531
Cdd:cd17654 338 GTMRA--------TGD--FVTvKDGELFFLGRKDSQIKRRGKRINLDLIQQVIESCLGV-ESCAVTLSDQQ-----RLIA 401
|
330 340 350 360
....*....|....*....|....*....|....*....|....*...
gi 479816261 532 VRRDPELTEDEVKAFCAERLTNYKRPREVEFRESLPKSNVGKILRREL 579
Cdd:cd17654 402 FIVGESSSSRIHKELQLTLLSSHAIPDTFVQIDKLPLTSHGKVDKSEL 449
|
|
| AACS |
cd05943 |
Acetoacetyl-CoA synthetase (acetoacetate-CoA ligase, AACS); AACS is a cytosolic ligase that ... |
75-257 |
5.76e-07 |
|
Acetoacetyl-CoA synthetase (acetoacetate-CoA ligase, AACS); AACS is a cytosolic ligase that specifically activates acetoacetate to its coenzyme A ester by a two-step reaction. Acetoacetate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is the first step of the mevalonate pathway of isoprenoid biosynthesis via isopentenyl diphosphate. Isoprenoids are a large class of compounds found in all living organisms. AACS is widely distributed in bacteria, archaea and eukaryotes. In bacteria, AACS is known to exhibit an important role in the metabolism of poly-b-hydroxybutyrate, an intracellular reserve of organic carbon and chemical energy by some microorganisms. In mammals, AACS influences the rate of ketone body utilization for the formation of physiologically important fatty acids and cholesterol.
Pssm-ID: 341265 [Multi-domain] Cd Length: 629 Bit Score: 52.27 E-value: 5.76e-07
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 75 DISYKELDDHSRALAAWLQSRGLVKGDRVAIMMPNILQYPITFTAVLRAGFVVVNVNPlytprelehqlnDAGAKAlvVL 154
Cdd:cd05943 98 EVTWAELRRRVARLAAALRALGVKPGDRVAGYLPNIPEAVVAMLATASIGAIWSSCSP------------DFGVPG--VL 163
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 155 ENFAtTVEkalpsinvPNILVASMGDMLGFKGHIVNLVVRRVKKMVP------------------AWNIPGHVRFKDALA 216
Cdd:cd05943 164 DRFG-QIE--------PKVLFAVDAYTYNGKRHDVREKVAELVKGLPsllavvvvpytvaagqpdLSKIAKALTLEDFLA 234
|
170 180 190 200
....*....|....*....|....*....|....*....|.
gi 479816261 217 QGRAKSFNPVPVQGCDIAFLQYTGGTTGISKGAMLTHSNIL 257
Cdd:cd05943 235 TGAAGELEFEPLPFDHPLYILYSSGTTGLPKCIVHGAGGTL 275
|
|
| PLN03052 |
PLN03052 |
acetate--CoA ligase; Provisional |
76-151 |
2.88e-06 |
|
acetate--CoA ligase; Provisional
Pssm-ID: 215553 [Multi-domain] Cd Length: 728 Bit Score: 50.46 E-value: 2.88e-06
10 20 30 40 50 60 70
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 479816261 76 ISYKELDDHSRALAAWLQSRGLVKGDRVAIMMPNILQYPITFTAVLRAGFVVVNVNPLYTPRELEHQLNDAGAKAL 151
Cdd:PLN03052 209 MTLSELRSQVSRVANALDALGFEKGDAIAIDMPMNVHAVIIYLAIILAGCVVVSIADSFAPSEIATRLKISKAKAI 284
|
|
| ac_ac_CoA_syn |
TIGR01217 |
acetoacetyl-CoA synthase; This enzyme catalyzes the first step of the mevalonate pathway of ... |
61-581 |
4.86e-04 |
|
acetoacetyl-CoA synthase; This enzyme catalyzes the first step of the mevalonate pathway of IPP biosynthesis. Most bacteria do not use this pathway, but rather the deoxyxylulose pathway. [Central intermediary metabolism, Other]
Pssm-ID: 273507 [Multi-domain] Cd Length: 652 Bit Score: 42.95 E-value: 4.86e-04
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 61 RQFANSPAFTCMGKD-----ISYKELDDHSRALAAWLQSRGLVKGDRVAIMMPNILQYPITFTAVLRAGFVVVNVNPlyt 135
Cdd:TIGR01217 95 RAAGTEPALLYVDEThepapVTWAELRRQVASLAAALRALGVRPGDRVSGYLPNIPQAVVAMLATASVGAIWSSCSP--- 171
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 136 prelehqlnDAGAKAlvVLENFATTvekalpsinVPNILVASMGDMLGFKGHIVNLVVRRVKKMVP-------------- 201
Cdd:TIGR01217 172 ---------DFGARG--VLDRFQQI---------EPKLLFTVDGYRYNGKEHDRRDKVAEVRKELPtlravvhipylgpr 231
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 202 ---AWNIPGHVRFKDALAQGRAKS--FNPVPVQgcDIAFLQYTGGTTGISKGAMLTHSNILanVEQMnlwmdvafrnkgk 276
Cdd:TIGR01217 232 eteAPKIDGALDLEDFTAAAQAAElvFEQLPFD--HPLWILFSSGTTGLPKCIVHSAGGTL--VQHL------------- 294
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 277 pKALNFVCALPLYHIF--ALTVNAMI------GMKLGARNIL------IPNPRDIPSFVKELKkypVHIFpGLNTLFNGL 342
Cdd:TIGR01217 295 -KEHGLHCDLGPGDRLfyYTTTGWMMwnwlvsGLATGATLVLydgspgFPATNVLWDIAERTG---ATLF-GTSAKYVMA 369
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 343 MNNPDF---QTLDFKPLILTLGGGMAVqRPVAERWQH------MTGCHITEGyglseTSPVAC---ANALDATeFTGTIG 410
Cdd:TIGR01217 370 CRKAGVhpaRTHDLSALQCVASTGSPL-PPDGFRWVYdeikadVWLASISGG-----TDICSCfagANPTLPV-HIGEIQ 442
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 411 LPMPSTDVVIRDDDGSDlLLGKVGE-ICVRG-PQVMKGYWNRPE----ETARAIMADGFFRTGDMGFMDERGYTKIVDRK 484
Cdd:TIGR01217 443 APGLGTAVQSWDPEGKP-VTGEVGElVCTNPmPSMPIRFWNDPDgskyRDAYFDTYPGVWRHGDWITLTPRGGIVIHGRS 521
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 485 KDMILVSGFNVYPNEIEEVAAEHPGVLESAAVGIPNEHSGEVVKLYV-----VRRDPELTeDEVKAFCAERLTNYKRPRE 559
Cdd:TIGR01217 522 DSTLNPQGVRMGSAEIYNAVERLDEVRESLCIGQEQPDGGYRVVLFVhlapgATLDDALL-DRIKRTIRAGLSPRHVPDE 600
|
570 580
....*....|....*....|..
gi 479816261 560 VEFRESLPKSNVGKILRRELRD 581
Cdd:TIGR01217 601 IIEVPGIPHTLTGKRVEVAVKR 622
|
|
| PaaK |
cd05913 |
Phenylacetate-CoA ligase (also known as PaaK); PaaK catalyzes the first step in the aromatic ... |
463-510 |
6.15e-03 |
|
Phenylacetate-CoA ligase (also known as PaaK); PaaK catalyzes the first step in the aromatic degradation pathway, by converting phenylacetic acid (PA) into phenylacetyl-CoA (PA-CoA). Phenylacetate-CoA ligase has been found in proteobacteria as well as gram positive prokaryotes. The enzyme is specifically induced after aerobic growth in a chemically defined medium containing PA or phenylalanine (Phe) as the sole carbon source. PaaKs are members of the adenylate-forming enzyme (AFE) family. However, sequence comparison reveals divergent features of PaaK with respect to the superfamily, including a novel N-terminal sequence.
Pssm-ID: 341239 [Multi-domain] Cd Length: 425 Bit Score: 39.14 E-value: 6.15e-03
10 20 30 40 50 60
....*....|....*....|....*....|....*....|....*....|....*....|..
gi 479816261 463 FFRTGD--------------MGFMDergytKIVDRKKDMILVSGFNVYPNEIEEVAAEHPGV 510
Cdd:cd05913 292 RYRTRDitrllpgpcpcgrtHRRID-----RITGRSDDMLIIRGVNVFPSQIEDVLLKIPGL 348
|
|
| A_NRPS_alphaAR |
cd17647 |
Alpha-aminoadipate reductase; This family contains L-2-aminoadipate reductase, also known as ... |
74-544 |
6.30e-03 |
|
Alpha-aminoadipate reductase; This family contains L-2-aminoadipate reductase, also known as alpha-aminoadipate reductase (EC 1.2.1.95) or alpha-AR or L-aminoadipate-semialdehyde dehydrogenase (EC 1.2.1.31), which catalyzes the activation of alpha-aminoadipate by ATP-dependent adenylation and the reduction of activated alpha-aminoadipate by NADPH. The activated alpha-aminoadipate is bound to the phosphopantheinyl group of the enzyme itself before it is reduced to (S)-2-amino-6-oxohexanoate.
Pssm-ID: 341302 [Multi-domain] Cd Length: 520 Bit Score: 39.42 E-value: 6.30e-03
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 74 KDISYKELDDHSRALAAWLQSRGLVKGDRVAIMMPNILQYPITFTAVLRAGFVVVNVNPLYTPRELEHQLNDAGAKALVV 153
Cdd:cd17647 19 RSFTYRDINEASNIVAHYLIKTGIKRGDVVMIYSYRGVDLMVAVMGVLKAGATFSVIDPAYPPARQNIYLGVAKPRGLIV 98
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 154 LENFATTVekalpsinvpnilvasmgdmlgfkghivnlvvrrvkkmvpawnipghvrfkdalaqgrAKSFNPVpvqgcdi 233
Cdd:cd17647 99 IRAAGVVV----------------------------------------------------------GPDSNPT------- 113
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 234 afLQYTGGTTGISKGAMLTHSNILANVEqmnlWMDVAFR--NKGKPKALNFVCALPLYH-IFAltvnamiGMKLGARnIL 310
Cdd:cd17647 114 --LSFTSGSEGIPKGVLGRHFSLAYYFP----WMAKRFNlsENDKFTMLSGIAHDPIQRdMFT-------PLFLGAQ-LL 179
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 311 IPNPRDI--PSFVKE-LKKYPV---HIFPGLNTLFNGLMNNPdfqtldFKPLILTLGGGMAVQRPVAERWQHMT-GCHIT 383
Cdd:cd17647 180 VPTQDDIgtPGRLAEwMAKYGAtvtHLTPAMGQLLTAQATTP------FPKLHHAFFVGDILTKRDCLRLQTLAeNVRIV 253
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 384 EGYGLSET----------SPVACANALDATEFTGTIGLPMPSTD--VVIRDDDGSDLLLGKVGEICVRGPQVMKGYWNRP 451
Cdd:cd17647 254 NMYGTTETqravsyfevpSRSSDPTFLKNLKDVMPAGRGMLNVQllVVNRNDRTQICGIGEVGEIYVRAGGLAEGYRGLP 333
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 452 EETARAIMADGF----------------------------FRTGDMGFMDERGYTKIVDRKKDMILVSGFNVYPNEIEEV 503
Cdd:cd17647 334 ELNKEKFVNNWFvepdhwnyldkdnnepwrqfwlgprdrlYRTGDLGRYLPNGDCECCGRADDQVKIRGFRIELGEIDTH 413
|
490 500 510 520
....*....|....*....|....*....|....*....|...
gi 479816261 504 AAEHPGVLESAAVgIPNEHSGE--VVKLYVVRRDPELTEDEVK 544
Cdd:cd17647 414 ISQHPLVRENITL-VRRDKDEEptLVSYIVPRFDKPDDESFAQ 455
|
|
| PLN03051 |
PLN03051 |
acyl-activating enzyme; Provisional |
476-581 |
7.88e-03 |
|
acyl-activating enzyme; Provisional
Pssm-ID: 215552 [Multi-domain] Cd Length: 499 Bit Score: 39.03 E-value: 7.88e-03
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 479816261 476 GYTKIVDRKKDMILVSGFNVYPNEIEEVAAE-HPGVLESAAVGIPNEHSG-EVVKLYVVRRDPELT-----EDEVKAFCA 548
Cdd:PLN03051 371 GYFCVQGRADDTMNLGGIKTSSVEIERACDRaVAGIAETAAVGVAPPDGGpELLVIFLVLGEEKKGfdqarPEALQKKFQ 450
|
90 100 110
....*....|....*....|....*....|....*..
gi 479816261 549 ERLTNYKRP----REVEFRESLPKSNVGKILRRELRD 581
Cdd:PLN03051 451 EAIQTNLNPlfkvSRVKIVPELPRNASNKLLRRVLRD 487
|
|
| |
